• Here, using a peptide phage display library of enhanced size and diverse composition, we examined the binding specificity of a recombinant monoclonal antibody (mAb) constructed with the heavy chain and light chain variable domains of a CLL BCR that does not exhibit somatic mutations. (biomedcentral.com)
  • Moreover, the mAb-peptide interactions were of lower affinities (micromolar K D ), as measured by surface plasmon resonance, than those observed with a CLL mAb containing somatic mutations (nanomolar K D ) and with immunoglobulin heavy chain variable ( IGHV )-mutated antibodies selected by environmental antigens. (biomedcentral.com)
  • 1 In this manuscript, the authors describe the observation that immunoglobulin variable (V) region heavy and light chain transcripts from anti-citrullinated protein antibody (ACPA) IgG-expressing B cells in patients with rheumatic arthritis (RA) contain N-glycosylation sites (Nglycs) acquired by somatic hypermutation, whereas these acquired Nglycs (ac-Nglycs) were absent in tetanus toxoid (TT) specific B cells of healthy individuals. (bmj.com)
  • Molecular characterization of the Ig variable region from 320 sequences expressed by germ cell tumor-infiltrating B cells revealed clear evidence of Ag experience, in that the cardinal features of an Ag-driven B cell response were present: significant somatic mutation, isotype switching, and codon insertion/deletion. (jimmunol.org)
  • 26 27 In contrast, the chromosomal breakpoint in sBL and HIV-associated BL occurs most commonly in the class switch region, 28 but since both somatic hypermutation and class switching are events that are normally confined to GC B cells and GC centroblast markers are expressed on BL cells, the BL progenitor cells most likely arise from B cells subjected to chromosomal rearrangements in the GC. (bmj.com)
  • Interestingly, at distinct stages of T lineage development, both the increased and decreased rearrangement of particular Vδ gene segments is highly sensitive to the dosage of the E2A gene products, suggesting that the concentration of the E2A proteins is rate limiting for the recombination reaction involving these Vδ regions. (rupress.org)
  • There was more nucleotide changes occurred in complementary determining regions ( CDR) than those in the framework regions (FR) in the V-H and V-L of B cell clones. (bjmu.edu.cn)
  • The transfection of hammerhead ribozyme or the addition of chemically modified ribozyme against the flanking region of V3-7 caused a potent and selective inhibition of anti-DNA production in V3-7-using B cell clones, but not in irrelevant V gene-using clones in vitro. (jimmunol.org)
  • 19 ] Immunological aberrations, including Coombs-positive hemolytic anemia, immune thrombocytopenia, and depressed immunoglobulin levels may all complicate the management of CLL. (cancer.gov)
  • 3 A number of immunohistochemical studies have demonstrated that the immune cell infiltrate of many germ cell tumors includes substantial yet variable numbers of T cells, macrophages, and B cells ( 3 , 4 , 5 ). (jimmunol.org)
  • Analysis of the rearranged immunoglobulin variable region gene hypermutation has provided important information concerning the clonal history and ontogenetic origin of various B-cell lymphoproliferative disorders. (ovid.com)
  • The accuracy of the analysis is being tested and refined by the prediction of immunoglobulin structures prior to their experimental determination. (nih.gov)