• Design Expression of IL-33 and ST2 was determined by qRT-PCR, ELISA, immunohistochemistry and western-blot analysis. (bmj.com)
  • Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. (prosci-inc.com)
  • The high quality and purity of these antibodies make them valuable tools for applications including ELISA, immunohistochemistry, immunoblotting (Western blot) and immunoprecipitation techniques (including immunodiffusion and rocket immunoelectrophoresis). (cedarlanelabs.com)
  • See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more. (lsbio.com)
  • The changes of MMP-9 were examined by RT-PCR assay and Western blot. (scielo.br)
  • Results were compared with those of an immunofluorescence assay (IFA) for FCoV-specific antibody and correlated with health status. (avma.org)
  • The current HIV diagnostic algorithm consists of a repeatedly reactive immunoassay (IA), followed by a supplemental test, such as the Western blot (WB) or indirect immunofluorescence assay (IFA). (cdc.gov)
  • The biotinylated MP6-XT22 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified 6B8 antibody (Cat. (biolegend.com)
  • Al- direct demonstration or cultivation of the though a number of papers have reported causative organism, remain the definitive the existence of circulating antigens and laboratory diagnostic method for VL, sero- immune complexes in VL [ 16-19 ], none of diagnostic methods are needed to over- them has led to a useful antigen detection come the diagnostic difficulties assay for VL. (who.int)
  • Serologic tests include complement fixation (CF), indirect immunofluorescence (IFAT) and enzyme-linked immunosorbent assay (ELISA). (medscape.com)
  • Testing of IgG autoantibodies to human cellular antigens was performed by the HEp-2 cell immunofluorescence assay using slides from INOVA Diagnostics, San Diego, CA (Cat # 508100) following the manufacturer's instructions and evaluated primarily using their NOVA View system. (cdc.gov)
  • These cells were characterised by PCR, immunocytochemistry, electron microscopy, and Western and Northern blot analyses. (ersjournals.com)
  • Western blotting, immunohistochemical staining of paraformaldehyde-fixed, saponin-treated frozen tissue sections 7-9 in vivo detection 5 , immunofluorescence, and immunocytochemistry. (biolegend.com)
  • Use in Immunocytochemistry/immunofluorescence reported in multiple pieces of scientific literature. (novusbio.com)
  • These prob- mania antibodies have been described, lems could, theoretically, be avoided by de- including indirect immunofluorescence tecting antigen in the urine [ 20-22 ]. (who.int)
  • Immunofluorescence and Western blot analyses revealed that T. pyogenes infection also results in the damage of tight junction proteins in BEECs. (preprints.org)
  • Both confocal immunofluorescence staining and Western blot were used to detect the changes of LMP1 expression. (scielo.br)
  • In SK-N-SH cells, carbachol induced a rapid and transient translocation of tubulin to the plasma membrane, microtubule reorganization, and a change in cell shape as demonstrated by confocal immunofluorescence microscopy. (jneurosci.org)
  • ELISA(peptide): For ELISA detection of antigenic peptide. (affbiotech.com)
  • The resultant recombinant protein was produced and used in antibody detection assays via western blot analysis of serum samples. (avma.org)
  • Western blot and permeability assays were used to explore the role and mechanism of the antitumor effects of PCNs in vivo. (biomedcentral.com)
  • IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. (affbiotech.com)
  • EB09516 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. (everestbiotech.com)
  • antibody detection limit dilution 1:32000. (everestbiotech.com)
  • PA1-980) which works nicely in Western blots (ECL detection) at 1:2,000 and higher dilution. (bio.net)
  • The determination of autoantibody titers from 1:80 to 1:1280 was performed by serial dilution on samples that showed a 3+ or greater nuclear and/or cytoplasmic immunofluorescence pattern. (cdc.gov)
  • These antibodies are available in a variety of formats and can be used for flow cytometry, imaging, ELISA and western blot. (bdbiosciences.com)
  • Conclusions and Clinical Relevance -Expression of the 7b protein, as indicated by detection of antibodies against the protein, was found in most FCoV-infected cats. (avma.org)
  • The highly infectious phase of acute human immunodeficiency virus (HIV) infection, defined as the interval between the appearance of HIV RNA in plasma and the detection of HIV-1-specific antibodies, contributes disproportionately to HIV transmission ( 1 ). (cdc.gov)
  • Antibodies is the highest revenue contributor of this segment, increasing adoption of antibodies in detection, identification, and alteration/modification of proteins followed by their role in monitoring cell regulation & expression, driving the segment growth. (marketsandmarkets.com)
  • Broad applications of antibodies in epigenetics research across different techniques such as, immunofluorescence, western blotting, and ChIP has propelled various market players to expand their product offerings around traditional as well as recombinant antibodies. (marketsandmarkets.com)
  • These antibodies have been tested for suitability for use in Western Blotting and ELISA. (cedarlanelabs.com)
  • In the past, tion, the detection of antigens in patient se- many patients in Sudan died untreated in rum is complicated by the presence of high hospital, with the diagnosis of VL being levels of antibodies, circulating immune made later at autopsy [ 6 ]. (who.int)
  • A variety of sero- complexes, serum amyloid, rheumatoid logical tests for the detection of anti-leish- factors and autoantibodies. (who.int)
  • No. 516003) as the detection antibody and recombinant mouse TNF-α (Cat. (biolegend.com)
  • Detection limit for recombinant GST tagged TAF6L is 0.1 ng/ml as a capture antibody. (abnova.com)
  • BD Biosciences tools use multiple methodologies including flow cytometry , bioimaging and microscopy (for live and fixed cell analysis) as well as ELISA , IHC , western blot and spectrofluorometry. (bdbiosciences.com)
  • Fluorescence activated cell sorting was used to isolate FSCs from CNT-treated normal human lung fibroblasts (NHLFs).The expression of stem cell markers and fibrogenic markers was examined using western blotting, immunofluorescence staining and confocal microscopy. (cdc.gov)
  • Active caspases can then cleave many proteins, including poly-ADP ribose polymerase (PARP), other caspases and other protein substrates en masse. (bdbiosciences.com)
  • Moreover, the detection of the anti-apoptotic protein Bcl-2 and apoptotic proteins BAX, cytochrome c, as well as the activating effector caspase-3 revealed that T. pyogenes induced apoptosis of BEECs, which was also confirmed by DAPI staining to observe the morphological changes of the nuclei of cell apoptosis and by TUNEL staining to locate the cells undergoing apoptosis. (preprints.org)
  • The expression of microtubule-associated protein 1 light chain 3 (LC3) in gastric antrum and ileum was detected by Immunofluorescence staining. (bvsalud.org)
  • The levels of LC3II, P62/SQSTM1, PTEN-induced putative protein kinases (PINK1), E3 ubiquitin ligase (Parkin), AMP-dependent protein kinases (AMPK), phosphorylated AMPK (p-AMPK), nuclear factor erythroid 2-related factor (Nrf2) and kelch like ECH Associated Protein 1 (Keap1) in gastric antrum and ileum were assayed by western blotting. (bvsalud.org)
  • WB: For western blot detection of denatured protein samples. (affbiotech.com)
  • The use of an antigen detection test states [ 1-5 ]. (who.int)
  • Tests for the detection of antigen of crucial importance, as a successful out- would, in principle, provide a better means come depends on the initiation of prompt of diagnosis since the presence of antigen treatment. (who.int)
  • In addition, hippocampal dystrophin levels were estimated by Western blot analysis in biopsies from TLE patients, post-mortem controls, amygdala kindled (AK)-, and control rats. (frontiersin.org)
  • dc.title: PCR based detection techniques and DNA finger printing by restriction fragment analysis of mycobacterium tuberculosis dc.contributor.author: MAGANAARACHCHI, DN dc.description.abstract: In Sri Lanka definitive diagnosis of tuberculosis depends on the culture of mycobacteria, but the slow growth of the organism delays the diagnosis. (who.int)
  • Examination of direct smears for acid fast bacilli is the widely used method for the detection of mycobacteria, but lacks the desired specificity and relatively large number of bacteria is needed for detection. (who.int)
  • In conclution, DNA amplification is a rapid, reliable and accurate method with a high degree of sensitivity and specificity for the detection of Mycobacterium tuberculosis DNA sequences and it can replace the conventional culture method in the diagnosis of extra pulmonary tuberculosis and tuberculosis meningitis except in the situation when antibiotic sensitivity results are required. (who.int)
  • which would detect EGFP even in a denatured form (for westerns for example)? (bio.net)
  • Although the molecular detection of viral nucleic acid by reverse transcription PCR (RT-PCR) is the standard for MERS-CoV diagnosis, serologic detection remains necessary. (cdc.gov)
  • A Western blot confirmatory test has been described, to address the concern for serologic cross-reactivity. (medscape.com)
  • NEW YORK - Beverly, Massachusetts-based startup TellBio aims to use its circulating tumor cell, or CTC, collection technology for early detection of tumor progression and to develop therapeutics based on CTC biomarkers. (genomeweb.com)
  • and immunofluorescence and cell imaging in prostate cancer. (genomeweb.com)
  • Western Blot: ITPR2 Antibody [NB100-2466] - 2 sec exposure using NB100-2466 on 80 ug total cell lysates listed above. (novusbio.com)
  • IF/ICC: For immunofluorescence detection of cell samples. (affbiotech.com)
  • To support this spectrum of requirements, BD Biosciences offers a full range of apoptosis detection tools and technologies for measuring indicators at different stages across the apoptotic process. (bdbiosciences.com)
  • 20% sensitive, even using immunofluorescence techniques. (msdmanuals.com)
  • 2 IgM Western Blot (WB) is considered positive when at least two of the following three bands are present: 24 kDa (OspC)*, 39 kDa (BmpA), and 41 kDa (Fla). Low incidence states should disregard IgM results for specimens collected >30 days after symptom onset. (cdc.gov)
  • KAtex est un simple supplément pour le diagnostic de la leishmaniose viscérale, en particulier sur le terrain, et un test complémentaire pour le diagnostic de la leishmaniose viscérale dans les cas à frottis négatif ayant des résultats positifs au test d'agglutination directe. (who.int)
  • TellDx, which Gandhi noted requires 10 mL of a patient's blood, can isolate CTCs within three hours prior to downstream detection methods such as PCR and sequencing. (genomeweb.com)
  • This novel finding provides a new insight into the mechanisms of fibrosis and may aid in the development of early detection biomarkers and treatment strategies for the disease. (cdc.gov)
  • For skin regenerative effects, skin wound mice model was exploited to analyze wound-healing rate in this study, as well as immunofluorescence and histological staining evaluates. (biomedcentral.com)
  • Blood banks in areas of endemic disease use either the IHA or LA tests for disease detection. (medscape.com)
  • Score>80(red) has high confidence and is suggested to be used for WB detection. (affbiotech.com)