Indirect immunofluorescenceWestern BlotMitosisActinCleavageSegregationAssaysMidbodyAnalysesCellsMitotic spindleHeLaCellLocalizationMyosinMembraneRegionFailureFoundAppearsMitosis and cytokinesisMidbody in cytokinesisSpindleAssayMicroscopyActomyosinMicrotubuleExperimentsAntibodyGeneEssentialAnalysisImages
Indirect immunofluorescence7
- A comparison of the distribution of actin and tubulin in the mammalian mitotic spindle as seen by indirect immunofluorescence. (escholarship.org)
- Rabbit antibodies against actin and tubulin were used in an indirect immunofluorescence study of the structure of the mitotic spindle of PtK1 cells after lysis under conditions that preserve anaphase chromosome movement. (escholarship.org)
- Calmodulin was localized in Saccharomyces cerevisiae by indirect immunofluorescence using affinity-purified polyclonal antibodies. (rupress.org)
- a) Representative images of HEK 293 cells stained for endogenous HIPK2 (green) and PARP1 (red) by indirect immunofluorescence (endo, endogenous). (scienza-under-18.org)
- HEK 293 cells were transfected with expression vectors encoding GFP-HIPK2 and analyzed for HIPK2 (green) and endogenous PARP1 (red) by indirect immunofluorescence (lower panels). (scienza-under-18.org)
- Interaction inferred from two proteins that co-localize in the cell by indirect immunofluorescence only when in addition, if one gene is deleted, the other protein becomes mis-localized. (thebiogrid.org)
- HeLa cells were processed for indirect immunofluorescence with antibodies to two nuclear pore components, ELYS (embryonic large protein derived from yolk sac) and mAb414 against the FG nucleoporins. (ucsd.edu)
Western Blot3
- 33-9100 has been successfully used in ELISA, Immunofluorescence and Western Blot analysis of ZO-1. (thermofisher.com)
- Suitable for use in ELISA, Immunofluorescence and Western Blot. (antibodyguide.com)
- Rabbit polyclonal antibody anti-Alpha-Tubulin is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence. (stjohnslabs.com)
Mitosis4
- Immunofluorescence analysis revealed that SOGA2/MTCL1 co-localizes with mitotic spindle microtubules and spindle poles throughout mitosis and both SOGA proteins are enriched at the midbody during mitotic exit/cytokinesis. (abberior.rocks)
- Cytokinesis begins after chromosome separation in mitosis and ends when the cell divides. (jove.com)
- Cytoskeleton morphogenesis is normally central to cell routine events such as for example organelle setting, segregation, cytokinesis and mitosis [3-5]. (isme-la2019.org)
- Methylation of alpha chains at Lys-40 is found in mitotic microtubules and is required for normal mitosis and cytokinesis contributing to genomic stability. (stjohnslabs.com)
Actin7
- Citron-K or its fly orthologue Sticky has been suggested to interact with several molecules in cytokinesis such as Kinesin-3 (KIF14), actin, myosin light chain, and anillin. (wikipedia.org)
- Cells fixed during cytokinesis show actin in the region of the cleavage furrow, while antitubulin reveals the fibrous spindle remnant that runs between daughter cells. (escholarship.org)
- The small GTP-binding proteins of the Rho family, consisting of the Rho, Rac, and Cdc42 subfamilies, are implicated in various cell functions, such as cell shape change, cell motility and cytokinesis, through reorganization of actin cytoskeleton. (yeastgenome.org)
- ROCK II regulates the formation of actin stress fibers and focal adhesions, cytokinesis, smooth muscle contraction, and the activation of c-fos serum response element. (antibodyguide.com)
- Among them, CD2AP encodes CD2-associated protein, a scaffold protein implicated in dynamic actin remodeling and membrane trafficking during endocytosis and cytokinesis. (gencat.cat)
- Actin is usually involved in cytokinesis, muscle contraction, and cell movement. (aatbio.com)
- Actin microfilaments constitute one of the 3 major cytoskeletal structures, and they participate in many important cellular processes including muscle contraction, cell motility, cytokinesis, vesicle and organelle movement, cell signaling, the establishment and maintenance of cell junctions and cell shape. (scientificlabs.co.uk)
Cleavage4
- Cytokinesis failure of Citron-K-depleted cells occurred after full ingression of the cleavage furrow, at the abscission stage. (wikipedia.org)
- A central question in understanding cytokinesis is how the cleavage plane is positioned. (biologists.com)
- Of these hubs, KIAA0133, SEPT1, KIAA1377, and CRMP-1 were localized to the midbody, whereas HTR3A and ICAM2 were associated with the cleavage furrow as examined by immunofluorescence. (nycu.edu.tw)
- The beginning of cytokinesis is marked by the appearance of a crease, called the cleavage furrow. (jove.com)
Segregation3
- Previously, we have shown that depletion of centrin1 in Trypanosoma brucei (T. brucei) displayed arrested organelle segregation resulting in loss of cytokinesis. (elsevierpure.com)
- They impact cytokinesis and chromatid segregation during the G2/M phase of cell division. (rndsystems.com)
- It mediates proper CHROMOSOME SEGREGATION and contractile ring function during CYTOKINESIS. (lookformedical.com)
Assays4
- Upon further investigation, an association between the mutated motif and KLC1 and KLC2 was no longer detected using both immunofluorescence and pull-down assays. (bhdsyndrome.org)
- Immunofluorescence and pull-down assays also demonstrated that a number of these proteins could bind KLC1 and KLC2 to differing extents. (bhdsyndrome.org)
- Colony formation, β-gal staining, immunofluorescence, comet and homologous recombination (HR) assays and RNA-Seq analysis were used to determine the effects of AP4 inactivation. (biomedcentral.com)
- Immunofluorescence, chromosome and micronuclei enumeration, MTT and colony formation assays were used to determine the effects of AP4 inactivation and target gene regulation on chromosomal instability (CIN) and drug sensitivity. (biomedcentral.com)
Midbody2
- Midbody, a transient organelle-like structure, is known as central for abscission and is indispensable for termination of cytokinesis. (nycu.edu.tw)
- This subproteome network construction not only sheds light on the intimate interactions of the midbody proteomes, but also prioritizes novel hubs or protein complexes that may govern the process of cytokinesis. (nycu.edu.tw)
Analyses3
- Immunofluorescence analyses determined the localization of citron-K and its behavior during cytokinesis. (wikipedia.org)
- Rho GDI was found in the cytosol by immunoblot and immunofluorescence microscopic analyses. (yeastgenome.org)
- Functional proteomics and immunofluorescence analyses of H3.1 and H3.3 revealed a highly conserved role for Nrp1 and Asf1 histone chaperones in nuclear influx of histones. (biomedcentral.com)
Cells10
- Microtubule disassembly was not seen in any of Citron-K depleted cells with cytokinesis failure. (wikipedia.org)
- Knockdown of SEPT1 and KIAA1377 resulted in increasing numbers of cytokinesis defect cells, suggesting these newly identified hubs play critical roles in cytokinesis progression. (nycu.edu.tw)
- Both the immunofluorescence assay and electron microscopy showed that TbCen2 and 3-deficient cells were enlarged in size with duplicated basal bodies, multinuclei and new flagella that are detached along the length of the cell body. (elsevierpure.com)
- The abnormal segregations ultimately led to aborted cytokinesis and hence affected growth in these cells. (elsevierpure.com)
- The final step of the cell cycle that divides a cell into two daughter cells is called cytokinesis. (jove.com)
- Cell death also occurred to a similar extent as MiTMAB treatment in those cells that had failed cytokinesis inside the presence from the cytokinesis inhi bitor, cytochalasin B. So, failure of cytokinesis appears for being toxic to cells. (parp1inhibitor.com)
- We upcoming sought to determine when following cytokinesis failure the cells have been committed to apoptosis by utilizing movement cytometry. (parp1inhibitor.com)
- HeLa cells undergo caspase mediated apoptosis exclusively following cytokinesis failure Apoptosis is characterized by activation of the caspase dependent pathway. (parp1inhibitor.com)
- Immunofluorescence analysis of C6 cells using Alpha-Tubulin rabbit polyclonal antibody (STJ11103687) at dilution of 1:100 (40x lens). (stjohnslabs.com)
- Immunofluorescence analysis of NIH/3T3 cells using Alpha-Tubulin rabbit polyclonal antibody (STJ11103687) at dilution of 1:100 (40x lens). (stjohnslabs.com)
Mitotic spindle2
- Protein Regulator of Cytokinesis 1 assists Kinesin-12 in a Kinesin-5 independent pathway of mitotic spindle assembly. (umich.edu)
- Salazar, B.M., Ohi, R. "Protein Regulator of Cytokinesis 1 assists Kinesin-12 in building a bipolar mitotic spindle in the absence of Kinesin-5. (umich.edu)
HeLa1
- From this it could be seen that mutating the WE/WD motif led to a significant reduction in viruses reaching the host HeLa cell membrane (as measured by two independent immunofluorescence experiments). (bhdsyndrome.org)
Cell2
- The GAP activity of Tum is required for cytokinesis: in its absence cytokinesis fails early even though Tum is present on microtubules at the cell equator where the furrow should form. (biologists.com)
- Cell multi-nucleation was assessed by immunofluorescence. (biomedcentral.com)
Localization1
- This suggests that the regulation of the interaction between KIF14 and Citron-K is important for Citron-K localization to exert its function but this interaction alone cannot accomplish cytokinesis fully. (wikipedia.org)
Myosin1
- Fission yeast Rng3p: an UCS-domain protein that mediates myosin II assembly during cytokinesis. (thebiogrid.org)
Membrane1
- 2012), which suggested that FLCN could be involved in membrane trafficking and cytokinesis. (bhdsyndrome.org)
Region1
- As the bud grows, calmodulin concentrates at the tip, then disperses, and finally concentrates in the neck region before cytokinesis. (rupress.org)
Failure2
- The results demonstrate that MiTMAB induced apoptosis occurs largely following cytokinesis failure. (parp1inhibitor.com)
- This again sug gests the induction of apoptosis is related with cytokinesis failure rather than as a result of generalised toxicity on the MiTMABs. (parp1inhibitor.com)
Found1
- The glide was previously HLCL-61 covered with poly-L-Lysine for 10 min as found in regular immunofluorescence methods C find [10]. (isme-la2019.org)
Appears1
- These oils, again unthinkable, have that the quarter of the S immunofluorescence in plants appears more unique to that Installing in standard cookies than in turn megagames. (renault.ua)
Mitosis and cytokinesis4
- Our results indicate that at least 12 kinesins are involved in mitosis and cytokinesis. (nih.gov)
- Immunofluorescence assay of PfCENH3 strongly suggests that P. falciparum centromeres cluster to a single nuclear location prior to and during mitosis and cytokinesis but dissociate soon after invasion. (unibas.ch)
- Introduction Temporal and spatial coordination of the process of mitosis and cytokinesis is a prerequisite for accurate and equal segregation of genomic and cytosolic material into two daughter cells. (forumbcn2004.org)
- Component of a chromosome passage protein complex (CPC) which is essential for chromosome alignment and segregation during mitosis and cytokinesis. (affbiotech.com)
Midbody in cytokinesis2
- Not surprisingly, in CEM cells Aurora B is maximally expressed in mitotic cells and localises to the midbody in cytokinesis, to the spindle midzone in anaphase/telophase and to centromeres in metaphase. (others-signal.com)
- pT182-Polo stainings of the centrosomes and the midbody in cytokinesis were strongly diminished, but never completely abolished, probably because cells that could complete mitosis were those for which Polo depletion was only partial. (innovation-ecosystems-agora.com)
Spindle1
- directs CPC movement to different locations from the inner centromere during prometaphase to midbody during cytokinesis and participates in the organization of the center spindle by associating with polymerized microtubules. (affbiotech.com)
Assay2
- Experience with de-condensation assays of chromosomes and competency in the cytokinesis block micronuclei assay are required. (nih.gov)
- A,C) Immunofluorescence assay of HFDPCs with DENV-1 (MOI = 10) and DENV-2 (MOI = 10 and 50) infections for 4 times. (mindunwindart.com)
Microscopy4
- Using immunofluorescence analysis, time-lapse microscopy, and rescue experiments, we investigate the roles of these 12 kinesins in detail. (nih.gov)
- Additionally, experience in Western blotting, Immunofluorescence, and the use of Light and Fluorescent Microscopy is needed. (nih.gov)
- Anti-tubulin antibodies and confocal immunofluorescence microscopy were used to examine the organization and regulation of cytoplasmic and cortical microtubules during the first cell cycle of fertilized Xenopus eggs. (biologists.com)
- Cellular morphology and cytoskeleton integrity of peptide-treated cells were observed by immunofluorescence microscopy. (mdpi.com)
Actomyosin1
- Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration. (arigobio.cn)
Microtubule3
- Microtubule disassembly was not seen in any of Citron-K depleted cells with cytokinesis failure. (wikipedia.org)
- Here, we show that Leishmania coronin preferentially distributes to the distal tip during cytokinesis, and interacts with microtubules through a microtubule-based motor, kinesin K39. (silverchair.com)
- These results indicate that coronin regulates microtubule remodeling during Leishmania cytokinesis and is essentially required for survival of these parasites in culture. (silverchair.com)
Experiments2
- These findings have been confirmed by immunofluorescence experiments exhibiting a significant maximize of multinucleate cells just after MK 0457 remedy, from 8. (srcpathway.com)
- In immunofluorescence experiments, GAP and Tid-1 were shown to colocalize at perinuclear mitochondrial membranes in response to epidermal growth factor stimulation. (embl.de)
Antibody1
- E) Specificity SM-130686 of the antibody against PoloT182Ph in immunofluorescence. (innovation-ecosystems-agora.com)
Gene1
- This defect in cytokinesis, however, disappears upon episomal gene complementation. (silverchair.com)
Essential1
- and Kif4A, Kif4B, MKLP1, and MKLP2 (members of the kinesin-6 family) are essential for cytokinesis. (nih.gov)
Analysis1
- Immunofluorescence (IF) analysis is a common clinical procedure used in studying the development and behavior of several chronic diseases. (biomedcentral.com)
Images1
- Staining was observed in a Zeiss epifluorescent microscope and immunofluorescence images were recorded by a CCD Spot Camera (Diagnostic Instruments Inc. USA). (biomedcentral.com)