• A European multicenter study of immunoblotting for the serodiagnosis of Lyme borreliosis showed considerable variation in results obtained from tests with a panel of 227 serum samples. (asm.org)
  • Immunoblotting is both sensitive and specific, has been in wide use in diagnostic laboratories, and in the United States has been recommended as a confirmatory test for the serodiagnosis of Lyme disease ( 5 ). (asm.org)
  • By using immunoblotting techniques, it is possible to determine the extent of aggrecan degradation and to identify the degradation products that have accumulated in the tissue, and immunohistochemistry allows the location of the aggrecan degradation to be established. (springer.com)
  • The authors would like to thank Lisa Lamplugh for technical assistance with the immunoblotting and immunohistochemistry and Guylaine Bédard for the artwork. (springer.com)
  • Methods and Results- Human cell cultures were treated with various cytokines, and PHD expression was examined using qRT-PCR and immunoblotting. (ahajournals.org)
  • Throughout this work, I systematically altered several experimental parameters that are often neglected or overlooked when immunoblotting. (sciencemag.org)
  • The premise of immunoblotting is simple, but execution is tricky, and there are many variations in the method that can affect the outcome ( 1 ). (sciencemag.org)
  • Immunoblotting can be tricky, that's my experience. (biology-online.org)
  • Sera specific for the 52 kDa peptide of the SS-A antigen (anti-52 kDa SS-A) were better detected by immunoblotting. (hku.hk)
  • The present study was intended to detect SIRT1 content and activity, nicotinamide (NAM) and nicotinamide adenine dinucleotide (NAD) in the spinal cord using immunoblotting or mass spectroscopy over time in mice following chronic constriction injury (CCI) or sham surgery. (plos.org)
  • Placental tissues (n=45) obtained from pregnant women with preterm and term preeclampsia, spontaneous preterm delivery, and normal term delivery were analyzed for potential GPBB expression by immunoblotting. (ahajournals.org)
  • Using reverse transcription-polymerase chain reaction and immunoblotting we report that human keratinocytes and the human keratinocyte cell line HaCaT express VR1. (aspetjournals.org)