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ImmunoblottingBlotting, WesternMolecular Sequence DataElectrophoresis, Polyacrylamide GelAmino Acid SequenceCross ReactionsMolecular WeightEnzyme-Linked Immunosorbent AssayAntibodies, BacterialAntibody SpecificityAntigens, BacterialBase SequenceAntibodies, MonoclonalRNA, MessengerFluorescent Antibody Technique, IndirectFluorescent Antibody TechniqueCloning, MolecularRabbitsEpitopesRecombinant ProteinsSerologic TestsCells, CulturedBacterial ProteinsImmunohistochemistryImmunoglobulin GImmunosorbent TechniquesCell LineIsoenzymesBacterial Outer Membrane ProteinsPhosphorylationMembrane ProteinsImmunoassayImmune SeraAntibodies, HelminthAntibodiesMicroscopy, ImmunoelectronBorrelia burgdorferi GroupEvaluation Studies as TopicReverse Transcriptase Polymerase Chain ReactionGene ExpressionSequence Homology, Amino AcidLyme DiseaseAntigens, HelminthSensitivity and SpecificityBlotting, NorthernImmunologic TechniquesRats, Sprague-DawleyImmunoglobulin MImmunoenzyme TechniquesCarrier ProteinsCattleEscherichia coliCell MembranePolymerase Chain ReactionTumor Cells, CulturedHepatitis AntibodiesPrecipitin TestsChromatography, AffinityTransfectionSignal TransductionDNA, ComplementarySubcellular FractionsElectrophoresis, Gel, Two-DimensionalGenes, BacterialHepatitis C AntibodiesRecombinant Fusion ProteinsImmunologic TestsAntibodies, ViralSpecies SpecificityMutationDNA PrimersAntigens, SurfaceRadioimmunoprecipitation AssayMice, Inbred BALB CKineticsLiverAutoantibodiesEnzyme ActivationTaeniaAntigens, FungalGnathostomaCysticercosisRestriction MappingTime FactorsGene Expression RegulationApoptosisAntigens, ProtozoanPlasmidsPeptide FragmentsCell Line, TumorCytosolTranscription, GeneticTissue DistributionKidneyAntigens, ViralLiberiaHepatitis CMicroscopy, ElectronSwineCell Nucleus

AssayAntibodySerumELISAAntibodiesLymePresenceDiagnosticsSensitivityTestSeraTestsAnalysisSamplesAbsenceUniqueMethodsAntibodyAntibodiesRecombinantEnzyme-immunoassayProteinPolyvalentFractionsPatternsWesternSamplesSpecificCellUniquePatients

Assay7

• The LINE Immunoblot assay is a strip-based method using recombinant proteins applied in well-defined positions to nitrocellulose strip by a micro-dispensing method. (fujirebio.com)
• RIBATM Recombinant immunoblot assay. (cdc.gov)
• Positive samples were confirmed by immunoblot assay. (who.int)
• 95% CI: 19.9-29.7): 80 patients tested positive and 74 were confirmed positive by immunoblot assay. (who.int)
• Positive samples were able to HCV infection than others because retested for confirmation using a commer- of history of blood transfusion, frequent cial immunoblot assay kit (INNOGENET- injections, partial immunosuppression and ICS® Ghent, Belgium). (who.int)
• An immunoblot assay has a sensitivity of 9 nM HDI adduct. (cdc.gov)
• So the aims of tory immunoblot assay (LiaTek-III) test. (who.int)

Antibody9

• Access to Echinococcus Antibody, Immunoblot is restricted. (medicaldatabase.com)
• The Lyme Disease Antibody (IgG), Immunoblot test contains 1 test with 11 biomarkers. (ultalabtests.com)
• The Lyme Disease IgG Antibody Immunoblot test, often referred to as the Lyme Western Blot, is a laboratory test used to detect antibodies against the bacterium Borrelia burgdorferi , the causative agent of Lyme disease. (ultalabtests.com)
• The Lyme Disease IgG Antibody Immunoblot test is often ordered as a confirmatory test when a preliminary screening test for Lyme disease, like the enzyme immunoassay (EIA), yields positive or equivocal results. (ultalabtests.com)
• Why is the Lyme Disease IgG Antibody Immunoblot test performed? (ultalabtests.com)
• The Lyme Disease IgG Antibody Immunoblot test is used to detect antibodies to Borrelia burgdorferi, the bacterium responsible for Lyme disease. (ultalabtests.com)
• How does the Lyme Disease IgG Antibody Immunoblot test differ from the initial EIA test for Lyme disease? (ultalabtests.com)
• The Lyme Disease IgG Antibody Immunoblot test, on the other hand, checks for specific patterns of antibody response to the bacteria, making it a more definitive confirmatory test. (ultalabtests.com)
• ELISA, immunofluorescence, immunoblot, etc.) that have been developed for screening persons carrying the viral antibody from patients with overt symptoms of AIDS or AIDS-RELATED COMPLEX. (bvsalud.org)

Serum2

• Helicobacter pylori LINE Immunoblot is for the qualitative detection of Helicobacter pylori specific IgA-/IgG- antibodies respectively in human serum. (fujirebio.com)
• HCV-specific antibodies were detected in 376 (67.3%) serum samples using third-gen- eration enzyme immunoassay and confirmatory immunoblot assays. (who.int)

ELISA2

• By providing the immunoblot in a microplate format, screening and confirmatory tests can be processed on one automated ELISA system and free capacities on the instrument can be used optimally. (euroimmun.de)
• An immunoblot test is done to confirm ELISA results. (medlineplus.gov)

Antibodies2

• In the immunoblot, antigens coated on membranes are used as a solid phase in order to detect specific antibodies in patient samples. (euroimmun.de)
• The immunoblot can identify antibodies to specific protein bands, which adds to the specificity of the diagnosis. (ultalabtests.com)

Lyme1

• Babesia ImmunoBlots join ImmunoBlots already available for Lyme disease, Tick-Borne Relapsing Fever, and Bartonella. (lu.ma)

Presence1

• The role of SIRT1 in the activation of HIF-2α and nuclear factor-κB (RelA/p65) during hypoxia in the presence or absence of SIRT1 was assessed using immunoblot analysis. (molvis.org)

Diagnostics1

• BioVendor Group (Brno, Czech Republic) is demonstrating its IVD solutions in the form of a CLIA platform, a new generation of a unique immunoblot test for effective multiplex diagnostics, Microblot-Array, or its comprehensive sequencing solution using the NGS method at MEDICA 2023. (labmedica.com)

Sensitivity1

• While both immunoblots yielded reasonable sensitivity and high specificity, we recommend the use of the sonicated immunoblot, which has a higher sensitivity when used to detect acute disease and produces fewer cross-reactions. (edu.pe)

Test1

• If this test yields positive or equivocal results, then the immunoblot test (both IgG and IgM) will typically be ordered for confirmation. (ultalabtests.com)

Sera1

• Well-defined control sera were utilized in the development of these diagnostic immunoblots, and possible cross-reactions were thoroughly examined. (edu.pe)

Tests2

• Join Dr. Joseph Burrascano, Jr. MD for an in-depth look at ImmunoBlot testing from IGeneX, and why this method of testing has proven superior to other tests such as Western blot and IFA. (lu.ma)
• immunoblot tests. (ampkpathway.com)

Analysis2

• immunoblot analysis. (rosalindfranklin.edu)
• Immunoblot analysis identified two plasminogen binding proteins of 57 and 42 kDa. (lu.se)

Samples1

• Samples can be used fresh for dosage and immunoblot/immunoprecipitation or stored at -80°C for up to 6 months. (bessererlab.org)

Absence1

• We developed a procedure for rapid screening of clonal cell lines for the deletion of a protein of interest following CRISPR/Cas9 targeting in the absence of selective pressure based on dot immunoblots. (biomedcentral.com)

Unique1

• BioVendor is also highlighting Microblot-Array (MBA), a new generation of unique, immunoblot arrays in a microtiter plate format. (labmedica.com)

Methods1

• Two simple Bartonella bacilliformis immunoblot preparation methods were developed. (edu.pe)

Antibody2

• A negative result indicates that the immunoblot evaluation for B. burgdorferi antibody demonstrates no antibodies unique to B. burgdorferi and is therefore not supportive of Lyme disease. (clevelandcliniclabs.com)
• A positive result indicates that the immunoblot evaluation for Lyme antibody is consistent with the presence of antibody produced by patients in response to infection by B. burgdorferi and suggests the presence of Lyme disease. (clevelandcliniclabs.com)

Antibodies4

• Immunoblot testing qualitatively examines with high specificity antibodies in a patient's specimen. (clevelandheartlab.com)
• An immunoblot identifies the specific proteins to which the patient's antibodies bind. (testcatalog.org)
• Per Centers for Disease Control and Prevention criteria, the Lyme IgG Immunoblot is interpreted as positive if IgG-class antibodies are detected to greater than or equal to 5 Borrelia burgdo rferi proteins, and the Lyme IgM Immunoblot is interpreted as positive if IgM-class antibodies are detected to greater than or equal to 2 B burgdorferi proteins. (testcatalog.org)
• Epitopes from certain B burgdorferi proteins (eg, p41) are conserved across other bacteria, which may lead to the detection of IgM- and/or IgG-class antibodies on the Lyme disease immunoblots in patients without Lyme disease. (testcatalog.org)

Recombinant1

• We evaluated the performance of an HTLV-I Western blot (immunoblot) spiked with recombinant p21e protein (p21e WB) as an alternative to current confirmatory methods. (nih.gov)

Enzyme-immunoassay1

• The ViraChip ® is an immunoblot based on an enzyme-immunoassay in a microarray format, carrying highly purified specific antigens bound to nitrocellulose at defined positions. (atrida.nl)

Protein3

• Holoprotein/total CYP2D6 protein ratio was markedly higher for purified CYP2D6.1 (71.0%) than that calculated for CYP2D6.1 Supersomes (35.5%), resulting in distinct linear calibration range (0.05-0.50 versus 0.025-0.25 pmol) that was determined by densitometric analysis of immunoblot bands. (aspetjournals.org)
• Likewise, purified CYP2D6.2 and CYP2D6.10 and the CYP2D6.10 Supersomes all showed different holoprotein/total CYP2D6 protein ratios and distinct immunoblot linear calibration ranges. (aspetjournals.org)
• Because immunoblot and nano-LC/MS analyses measure total P450 protein (holoprotein and apoprotein) in a sample, complete understanding of holoprotein and apoprotein contents in P450 standards is desired toward reliable quantification. (aspetjournals.org)

Polyvalent1

• Confirmatory test: FTA-ABS-test (polyvalent) or Immunoblot. (fujirebio.com)

Fractions1

• Western immunoblots demonstrated decreased free ubiquitin in airway-enriched fractions. (cdc.gov)

Patterns1

• Immunoblot patterns not meeting these criteria should not be interpreted as positive. (testcatalog.org)

Western3

• Evaluation of a p21e-spiked western blot (immunoblot) in confirming human T-cell lymphotropic virus type I or II infection in volunteer blood donors. (nih.gov)
• Kleinman SH, Kaplan JE, Khabbaz RF, Calabro MA, Thomson R, Busch M. Evaluation of a p21e-spiked western blot (immunoblot) in confirming human T-cell lymphotropic virus type I or II infection in volunteer blood donors. (nih.gov)
• Western immunoblots identified shifts in ubiquitin pools. (cdc.gov)

Samples2

• Both IgM and IgG immunoblots should be performed on samples obtained less than 4 weeks after appearance of erythema migrans. (clevelandcliniclabs.com)
• Only IgG immunoblot is to be performed on samples greater than 4 weeks after disease onset. (clevelandcliniclabs.com)

Specific1

• In contrast to immunoblot, nano-LC/MS readily distinguished CYP2D6.2 (R296C and S486T) from CYP2D6.1 by isoform-specific proteolytic peptides that contain the altered amino acid residues. (aspetjournals.org)

Cell1

• We will characterize the new CYP3A5*6 cell lines by RT-PCR and immunoblot. (nih.gov)

Unique2

• BioVendor Group (Brno, Czech Republic) is demonstrating its IVD solutions in the form of a CLIA platform, a new generation of a unique immunoblot test for effective multiplex diagnostics, Microblot-Array, or its comprehensive sequencing solution using the NGS method at MEDICA 2023. (labmedica.com)
• BioVendor is also highlighting Microblot-Array (MBA), a new generation of unique, immunoblot arrays in a microtiter plate format. (labmedica.com)

Patients1

• Le contenu de ce site web est en partie réservé aux professionnels de santé qui sont directement impliqués dans la fourniture de services de soins de santé aux patients. (fujirebio.com)