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Adoptive TransferMice, Inbred C57BLImmunization, PassiveSpleenMice, KnockoutMice, Inbred BALB CCD4-Positive T-LymphocytesT-LymphocytesImmunity, CellularCD8-Positive T-LymphocytesImmunotherapy, AdoptiveMice, TransgenicLymphocyte ActivationImmune ToleranceT-Lymphocyte SubsetsT-Lymphocytes, RegulatoryImmunologic MemoryDendritic CellsB-LymphocytesImmunityFlow CytometryMice, Inbred NODOvalbuminImmunity, InnateLymph NodesMice, SCIDInterferon-gammaMice, Inbred StrainsT-Lymphocytes, CytotoxicEpitopes, T-LymphocyteDisease Models, AnimalCytokinesCells, CulturedGene Transfer TechniquesCell MovementCell DifferentiationDiabetes Mellitus, Type 1Fluorescence Resonance Energy TransferHypersensitivity, DelayedAdaptive ImmunityEmbryo TransferAntibody FormationMolecular Sequence DataImmunity, MucosalTh1 CellsImmunity, Maternally-AcquiredAutoimmune DiseasesAntigensGene Transfer, HorizontalCytotoxicity, ImmunologicGenetic VectorsLymphocyte TransfusionTh2 CellsImmunoglobulin GImmunity, HumoralCell LineRats, Inbred LewDose-Response Relationship, ImmunologicLymphocyte DepletionAmino Acid SequenceTime FactorsImmunosuppressionKiller Cells, NaturalThymus GlandMice, Inbred C3HMice, Inbred CBAReceptors, Antigen, T-CellEnergy TransferImmunophenotypingConjugation, GeneticInterleukin-10Dermatitis, ContactMacrophagesRadiation ChimeraImmunizationT-Lymphocytes, Helper-InducerBase SequenceAntigen PresentationPlasmidsAntibodies, ViralMelanoma, ExperimentalSignal TransductionPlant ImmunityMice, Mutant StrainsMice, Inbred DBAInterleukin-4KineticsLungMembrane GlycoproteinsCell ProliferationAntigens, CDInterleukin-12Bone Marrow CellsTransfer (Psychology)Antibodies, MonoclonalInflammationEpitopesEncephalomyelitis, Autoimmune, ExperimentalAntigens, CD4B-Lymphocyte Subsets
ImmunoTherapyAdoptively transferredAnti-tumor immunityTumor-specificTransgenic miceAntitumor activityMiceAdaptiveSuppressesVaccineEffectorMediateMelanomaInducesMurineIntracellularCellularAbstractHumoralResponsesSuppressSystemicInduceLymphodepletionCancerCivil liabilityVivoLargelyMechanismsClinical applicationDominantAntibodyTherapeuticLeukemiaTherapyGrowthStudiesSpecificFunctionImprove
ImmunoTherapy4
  • To apply allogeneic Vγ9Vδ2 T cells in adoptive immunotherapy, the methodology used to obtain adequate cell numbers with optimal effector function in vitro needs to be optimized, and clinical safety and efficacy also need to be proven. (nature.com)
  • T cell-based immunotherapy, such as immune checkpoint blockade or adoptive T cell transfer, is limited by the ability of T cells to detect major histocompatibility complex (MHC)-presented antigen by tumor cells. (elifesciences.org)
  • Immunotherapy of cancer envisions the adoptive transfer of T-cells genetically engineered with tumor-specific heterodimeric α/β T-cell receptors (TCRα/β). (oncotarget.com)
  • Lymphodepletion augments adoptive cell transfer during antitumor immunotherapy, producing dramatic clinical replies in sufferers with malignant most cancers. (irjs.info)
Adoptively transferred5
  • Results Herein we reveal a new way to reverse the tolerant state of adoptively transferred CD8 + T cells against tumors using TLR-activated B cells. (bmj.com)
  • Here we report that while exogenous administration of LPS was able to enhance adoptively transferred CD8 + T cells' tumor destruction, LPS treatment alone did not replace individual components of the tripartite ACT regimen, or obviate TBI. (biomedcentral.com)
  • furthermore, the expanded γδ T cells were adoptively transferred into late-stage liver and lung cancer patients. (nature.com)
  • Magnetic targeting of adoptively transferred tumour-specific nanoparticle-loaded CD8+ T cells does not improve their tumour infiltration in a mouse model of cancer but promotes the retention of these cells in tumour-draining lymph nodes. (csic.es)
  • Once adoptively transferred into CD19+ tumor-bearing mice, Akti treated CD19CAR T cells exhibited more antitumor activity than did untreated CD19CAR T cells. (biomedcentral.com)
Anti-tumor immunity14
  • CD4+ T cells promote anti-tumor immunity through numerous mechanisms, including enhancing antigen presentation, co-stimulation, T cell homing, T cell activation, and effector function. (wikipedia.org)
  • citation needed] Recent studies show the crucial role of proliferating activated effector memory Th1 CD4+ T cells in effective anti-tumor immunity and reveal that CD4+ T cells induce more durable immune-mediated tumor control than CD8+ T cells. (wikipedia.org)
  • furthermore, recent reports suggest that immunization (using either adjuvant or dendritic cells with pure tumor peptides) can result in productive anti-tumor immunity that is restricted by MHC class I. Finally, elimination of CD8+ T cells from mice at least partially abrogates anti-tumor immunity induced by most cancer vaccines. (wikipedia.org)
  • Similarly, a critical role for CD4+ T cells in induced anti-tumor immunity has been consistently demonstrated in vaccine/challenge experiments employing antibody-mediated depletion of CD4+ T cells or using CD4-knockout mice. (wikipedia.org)
  • Abrogation of anti-tumor immunity in CD4-knockout mice or mice depleted of CD4+ T cells has been demonstrated in cases of cell-based vaccines, recombinant viral vaccines and recombinant bacterial vaccines. (wikipedia.org)
  • While most adoptive transfer experiments have been performed with tumor-specific CD8+ T cells, activated CD4+ T cell clones specific for the murine leukemia virus have been demonstrated to confer systemic anti-tumor immunity upon transfer into tumor-bearing hosts. (wikipedia.org)
  • The effect of MTM analogues on hematological cancers and anti-tumor immunity: The LLS grant was focused on one analogue of mithramycin (MTM), designated MTM23. (uky.edu)
  • The LLS application was based our novel finding that MTM23 suppressed IL-10 production by leukemia cells and in preliminary studies enhanced anti-tumor immunity. (uky.edu)
  • The analogues developed in Aims 1 and 2 will be tested for their ability to inhibit secretion of IL-10, a cytokine that suppresses T-cell responses required for anti-tumor immunity. (uky.edu)
  • 15-color flow cytometry will be used to define the changes in specific T-cell subsets, with an emphasis on effector or memory cell status to understand the effects of the analogues on anti-tumor immunity. (uky.edu)
  • Determine selectivity, cytotoxicity, pharmacology and efficacy (Leggas) of MTM analogues in cells, mice and tumor models of Ewing sarcoma Assess if MTM analogues affect non-Ewing cancer cells with an emphasis on hematological cancers and if they enhance anti-tumor immunity. (uky.edu)
  • We will test if other MTM analogues have dual role in cancer by directly inhibiting cancer cells and enhancing anti-tumor immunity. (uky.edu)
  • Chronic lymphocytic leukemia derived interleukin-10 suppresses anti-tumor immunity. (uky.edu)
  • All FcgammaRs can crosslink anti-41BB antibodies to strengthen co-stimulation, but activating FcgammaR-induced antibody-dependent cell-mediated cytotoxicity compromises anti-tumor immunity by deleting 4-1BB(+) cells. (bioxcell.com)
Tumor-specific1
  • In contrast, administering LPS after ACT potentiated the antitumor effectiveness of the regimen, thereby supporting the expansion of transferred tumor-specific CD8 + T cells over host CD4 + T cells. (biomedcentral.com)
Transgenic mice4
  • Cure of mammary carcinomas in Her-2 transgenic mice through sequential stimulation of innate (neoadjuvant interleukin-12) and adaptive (DNA vaccine electroporation) immunity. (unich.it)
  • Pathologic findings, in vitro tests, and the results from immunization of both IFN-gamma and immunoglobulin gene knockout transgenic mice and of adoptive transfer experiments all show that IL-12 augments the B- and T-cell response elicited by vaccination and slightly decreases the number of regulatory T cells. (unich.it)
  • CONCLUSIONS: In Her-2 transgenic mice, IL-12 impairs tumor progression and triggers innate immunity so markedly that DNA vaccination becomes effective at late points in time when it is ineffective on its own. (unich.it)
  • Moreover, lymphocytes collected from recipients transferred by lymphocytes from transgenic mice revealed less proliferative potential than lymphocytes obtained from control recipients. (diabetesjournals.org)
Antitumor activity1
  • We now investigate whether inhibition of Akt signaling during ex vivo expansion of CAR T cells can promote the generation of CAR T cells with enhanced antitumor activity following adoptive therapy in a murine leukemia xenograft model. (biomedcentral.com)
Mice9
  • Calderon and Hay (l984 a,b) induced acute and chronic infection in BALB/c mice with Tricophyton quinckeanum and investigated immune responses by lymphocyte transformation and cell transfer experiments. (ivoryresearch.com)
  • The transfer of lymphocytes or ethylene di-isocyanate (HDI), which are used in a serum from sensitized mice can cause clinical disease in naive variety of industries including polyurethane foam mice. (cdc.gov)
  • B16F10-bearing mice were preconditioned with 5Gy TBI and given a tripartite ACT therapy (consisting of transferred pmel-1 CD8 + T cells, vaccination with fowlpox encoding gp100, and IL-2) along with TLR4 agonist LPS. (biomedcentral.com)
  • The experiments will consist of injecting the selected MTA analogue and antibody to PD-1 or PD-L1 or CTLA-4 into mice bearing the tumor and tumor primed T cells or CAR T-cells. (uky.edu)
  • Two additional sets of animals were used to examine the involvement of gut microbiota on alcohol-induced MAIT cell changes: (1) Cecal microbiota from alcohol-fed (AF) mice were adoptive transferred into antibiotic-pretreated mice and (2) AF mice were treated with antibiotics during the experiment. (lsuhsc.edu)
  • Mice that received OVA-specific OT-I TCR transgenic cells by adoptive transfer showed increased CD8+ T cell proliferation, cytokine production, and cytotoxic activity upon inclusion of SMIP2.1 Santacruzamate A in the Santacruzamate A OVA vaccine formulation. (cckn-ia.org)
  • Anti-tumor activity was evaluated after adoptive transfer of the CD19CAR T cells into CD19+ tumor-bearing immunodeficient mice. (biomedcentral.com)
  • However, our previous study in mice showed that linked nonimmunogenic Ags (OVA and GFP) containing immunodominant peptides mount immunity irrespective of the MHC-matched allogeneic cell's immunogenicity. (aai.org)
  • Consequently, we further explored 1) under what circumstances does the congenic marker CD45.1 provoke immunity in CD45.2 mice, and 2) whether linking two dominant or subdominant Ags can instigate an immune response. (aai.org)
Adaptive2
  • A main focus of immuno-oncology research is investigating immune checkpoint pathway functionality in effector killing cells, such as CD8+ T cell and natural killer (NK) cells, which regulate both adaptive and innate immunity. (thermofisher.com)
  • MAIT cell dysfunction may contribute to alcohol-induced innate and adaptive immunity and consequently end-organ pathophysiology. (lsuhsc.edu)
Suppresses1
Vaccine1
  • Thus, monocyte depletion by gemcitabine administration reduced the generation of these DC and increased vaccine-induced immunity, which rejected about 20% of LLC-OVA and B16-OVA tumors, which are non-responders to anti-PD-1. (unav.edu)
Effector4
  • Although numerous secreted effector proteins that reprogram innate immunity to promote virulence have been identified in pathogenic bacteria, so far, there are no examples of analogous secreted effector proteins produced by human fungal pathogens. (bvsalud.org)
  • This work identifies a circuit whereby a secreted effector protein produced by a human fungal pathogen reprograms innate immunity, revealing an unexpected role for Toll-like receptor 4 in promoting the pathogenesis of infectious disease. (bvsalud.org)
  • Insufficient persistence and effector function of chimeric antigen receptor (CAR)-redirected T cells have been challenging issues for adoptive T cell therapy. (biomedcentral.com)
  • Trained immunity of natural killer (NK) cells has shown great potential in the treatment of cancers by eliciting enhanced effector responses to restimulation by cytokines or cancer cells for long time periods after preactivation. (cancerbiomed.org)
Mediate1
Melanoma4
  • To determine the mechanisms of how innate immune activation via lymphodepletion potentiated antitumor T cell immunity, we utilized the pmel-1 melanoma mouse model. (biomedcentral.com)
  • These findings suggested that Notch1 signaling in B16 melanoma cells might inhibit antitumor immunity by upregulation of TGF-β1. (biomedcentral.com)
  • There are compelling evidences to show that melanoma cells escape the host's immunity by actively developing multiple suppressive mechanisms within the cancer microenvironment [ 1 ]. (biomedcentral.com)
  • Adoptive cell therapy using engineered T-cell receptors (TCRs) targeting cancer-testis antigens, such as Melanoma-associated antigen 3 (MAGE-A3), is a potential approach for the treatment of NSCLC. (thno.org)
Induces1
  • Intracellular Helicobacter pylori induces temporal waves in macrophage immunity. (nimml.org)
Murine2
  • The development of cell-mediated immunity during experimental murine dermatophytosis has been studied. (ivoryresearch.com)
  • High-avidity CTL are thought to be ideal for adoptive CTL transfer therapy as they have a proven ability to control tumour growth and Wooldridge with murine models. (colinsbraincancer.com)
Intracellular1
Cellular2
  • The identification of CMV-CTL clones is one method used to assess cellular immunity against CMV. (nature.com)
  • However, these reports have focused on the entire non-specific immune diversity, and not directly on the cellular immunity specific to virus. (nature.com)
Abstract1
  • pii: tpc.00974.2018 Peñuelas M, Monte I, Schweizer F, Vallat A, Reymond P, García-Casado G, Franco-Zorrilla JM, Solano R. Abstract Jasmonoyl-isoleucine regulates plant immunity, growth and development in vascular plants by. (csic.es)
Humoral1
  • Although many experimental adjuvants have been evaluated in animal models, until 10 y ago only squalene-based oil in water emulsions and aluminum-based salt adjuvants had been licensed for inclusion in human vaccines.1 These adjuvants are effective at eliciting humoral responses, but fail to stimulate CD8+ T cell immunity. (cckn-ia.org)
Responses1
  • None of the iMC from the different tumor models suppressed CTL responses in adoptive cell transfer experiments, unless GM-CSF was provided in vivo. (mdc-berlin.de)
Suppress1
Systemic1
  • 3. Tag-activation through Cre recombinase-encoding viruses in the liver and HCC development with systemic anti-Tag CTL immunity. (mdc-berlin.de)
Induce3
  • Using and experiments we showed that SMIP2.1 can activate the innate immune system via a TLR2-dependent mechanism, induce the maturation of APCs, and elicit a strong antibody response against influenza and tetanus toxoid antigens. (cckn-ia.org)
  • Agonistic anti-4-1BB antibodies have been reported to induce T cell mediated antitumor immunity. (bioxcell.com)
  • Next we investigated how innate pattern recognition receptors (PRRs) may facilitate the recognition of H. pylori to subsequently induce classic molecular cascades that efficiently modulate immunity. (nimml.org)
Lymphodepletion1
  • Lymphodepletion enhances adoptive T cell transfer (ACT) therapy by activating the innate immune system via microbes released from the radiation-injured gut. (biomedcentral.com)
Cancer3
  • This approach involves collecting, manipulating, activating, and transferring a subject's immune cells to treat cancer. (thermofisher.com)
  • The in vivo models are designed to test the ability of the analogues to enhance T-cell immunity to the cancer cells. (uky.edu)
  • IL-10 inhibits anti-cancer immunity. (uky.edu)
Civil liability1
  • immunity from civil liability. (nv.us)
Vivo1
  • Manufacturing CAR T cells is a process that involves activation and ex vivo culture with IL-2 to facilitate CAR gene transfer and to achieve a therapeutic number of T cells. (biomedcentral.com)
Largely2
  • Recent evidence suggests mononuclear phagocytes are largely involved in shaping dominant immunity during infection mediating the balance between host tolerance and succumbing to overt disease. (nimml.org)
  • However, the human NK cells responsible for the generation and maintenance of trained immunity are largely unknown. (cancerbiomed.org)
Mechanisms1
Clinical application2
  • These methods and results can help us to better understand immune reconstitution following hematopoietic cell transplantation, leading to future studies on the clinical application of adoptive T-cell therapies. (nature.com)
  • A better and deeper understanding of immune reconstitution following allo-HCT is required for further clinical application of these adoptive T-cell therapies. (nature.com)
Dominant2
  • Conceptually, immunity is believed to mainly target dominant Ags when subdominant or nondominant Ags are linked within the same cell due to T cell interference. (aai.org)
  • These findings challenge the existing perception that immunity is predominantly elicited against dominant Ags when linked to subdominant or nondominant Ags. (aai.org)
Antibody1
  • Many investigators have failed to correlate antibody titre with the clinical picture or the development of immunity. (ivoryresearch.com)
Therapeutic1
  • Our study indicates that IL-27 or adoptive transfer of IL-27 loaded tDCs may be a new therapeutic approach in atherosclerosis. (hindawi.com)
Leukemia1
  • All the adoptive transfer conditions for leukemia cell growth and monitoring have already been established in the laboratory (Alhakeem et al 2018). (uky.edu)
Therapy3
  • Background Adoptive T cell transfer (ACT) therapy improves outcomes in patients with advanced malignancies, yet many individuals relapse due to the infusion of T cells with poor function or persistence. (bmj.com)
  • Comprehensive solutions to support CAR T-cell therapy research from T-cell engineering to sequencing Adoptive cell transfer is a rapidly emerging area of immuno-oncology research. (thermofisher.com)
  • Adoptive cell therapy (ACT) has emerged as an effective approach in immuno-oncology research. (thermofisher.com)
Growth3
  • This finding provides a powerful adoptive immunization assay to follow the generation of concomitant antitumor immunity to progressive tumor growth. (grantome.com)
  • Experiments with the SA1 sarcoma, Meth A fibrosarcoma, P815 mastocytoma, P388 lymphoma, and L5178Y lymphoma revealed that progressive tumor growth evoked the generation of tumor-sensitized T cells that, on passive transfer, could cause the regression of an established tumor in a gamma-irradiated (500 rad) recipient. (grantome.com)
  • The generation and loss of T cells capable of adoptively immunizing against an established tumor corresponded temporally to the acquisition and loss of concomitant immunity to growth of a tumor cell implant. (grantome.com)
Studies1
Specific1
  • Transgenic animals have identified a role for specific manufacturing, auto body painting and repair and immunity, including the involvement of T-helper type 1/2 plastics manufacturing. (cdc.gov)
Function2
  • Additional experiments have revealed that suppressor T cells function in the adoptive immunization assay by inhibiting the ability of passively transferred helper or memory T cells to give rise to an adoptive cytolytic T-cell response in the tumor-bearing recipient. (grantome.com)
  • These electron transfer reactions are the main function of NAD. (bontac.com)
Improve1