• Clone 10.1 recognizes the EC3 epitope of CD64. (biolegend.com)
  • While both contain the EC3 domain, in-house testing suggests that clone 10.1 preferentially binds to CD64A (FcγRIA), but not CD64B (FcγRIB). (biolegend.com)
  • Human peripheral blood monocytes were stained with CD64 (clone 10.1) Brilliant Violet 711™ (filled histogram) or mouse IgG1, κ Brilliant Violet 711™ isotype control (open histogram). (biolegend.com)
  • These methods and results can help us to better understand immune reconstitution following hematopoietic cell transplantation, leading to future studies on the clinical application of adoptive T-cell therapies. (nature.com)
  • Cytomegalovirus reactivation is still a critical concern following allogeneic hematopoietic cell transplantation, and cellular immune reconstitution of cytomegalovirus-specific cytotoxic T-cells is necessary for the long-term control of cytomegalovirus reactivation after allogeneic hematopoietic cell transplantation. (nature.com)
  • However, cellular immune reconstitution of CMV-specific cytotoxic T-cells (CMV-CTL) is generally considered to be necessary for the long-term control of CMV reactivation after allo-HCT. (nature.com)
  • The recent development of next-generation sequence (NGS) technology has contributed to the high-throughput quantitative assessment of immune reconstitution following allo-HCT. (nature.com)
  • A better and deeper understanding of immune reconstitution following allo-HCT is required for further clinical application of these adoptive T-cell therapies. (nature.com)
  • Thus, we conducted immune- and RNA-sequencing of HLA-A24-restricted CMVpp65-specific CTLs to better understand the immune reconstitution of CMV-CTLs after allo-HCT. (nature.com)
  • We previously established a direct single-cell analysis to simultaneously identify and quantify in vivo CMV-CTL clones after allo-HCT 9 , 10 . (nature.com)
  • Anti-Siglec-8 antibody administration in vivo to humanized and transgenic mice selectively expressing Siglec-8 on mouse eosinophils and mast cells confirmed the in vitro findings, and identified additional anti-inflammatory effects. (mdpi.com)
  • The concept of enhancing cellular immunity through the transfer of ex-vivo expanded T cells was pioneered by Greenberg et al. (biomedcentral.com)
  • Here we show the features of repertoire diversity and the gene expression profile of HLA-A24 cytomegalovirus-specific cytotoxic T-cells in actual recipients according to the cytomegalovirus reactivation pattern. (nature.com)
  • Single-cell RNA-sequence analyses demonstrate the homogenous distribution of the gene expression profile in individual cytomegalovirus-specific cytotoxic T-cells within each recipient. (nature.com)
  • CD64 is a 72 kD single chain type I glycoprotein also known as FcγRI and FcR I. CD64 is a member of the immunoglobulin superfamily and is expressed on monocytes/macrophages, dendritic cells, and activated granulocytes. (biolegend.com)
  • CD64 binds IgG immune complex. (biolegend.com)
  • Human rheumatoid synovial fluid cells and fibronectin-purified monocytes. (biolegend.com)
  • Very low frequencies of collagen/matrix-specific CD4 + cells could be isolated from the PBMC or synovial fluid (SF) 4 of patients with rheumatoid arthritis (RA), using class II tetramers that contained two joint-specific Ags ( 5 ). (aai.org)
  • Bovine leukemia virus (BLV) infects cattle, integrates into host DNA as a provirus, and induces malignant B-cell lymphoma. (biomedcentral.com)
  • Also, the term "primary cutaneous CD4 + small/medium T-cell lymphoma" was changed to "primary cutaneous CD4 + small/medium T-cell lymphoproliferative disorder" because of its indolent clinical behavior and uncertain malignant potential. (medscape.com)
  • Siglecs (sialic acid-binding immunoglobulin-like lectins) are single-pass cell surface receptors that have inhibitory activities on immune cells. (mdpi.com)
  • Since its discovery in 2000, initial in vitro studies have found that the engagement of Siglec-8 with a monoclonal antibody or with selective polyvalent sialoglycan ligands induced the cell death of eosinophils and inhibited mast cell degranulation. (mdpi.com)
  • The bovine leukemia virus (BLV) belongs to the family Retroviridae (genus Deltaretrovirus ) and is closely related to human T-cell leukemia viruses (HTLV-1 and II) [ 1 ]. (biomedcentral.com)
  • Among these, Siglec-8 is a CD33-related family member selectively expressed on human mast cells and eosinophils, and at low levels on basophils. (mdpi.com)
  • Here we used an affinity-purification mass spectrometry-based (AP-MS) approach to identify novel and particularly intracellular sGAG-interacting proteins in human bone marrow stromal cells (hBMSC). (degruyter.com)
  • Furthermore, cross-reaction between the bacterial OspA and human LFA-1α L at the T cell level and the inability to detect Bb in the joint implicate an autoimmune mechanism. (aai.org)
  • To analyze the nature of response to OspA and LFA-1α L , we used OspA-specific T cell hybrids from DR4 transgenic mice, as well as cloned human cells specific for OspA 165-184 , the immunodominant epitope, from five DRB1*0401 + patients, using OspA-MHC class II tetramers. (aai.org)
  • Although OspA 165-184 stimulated nearly all OspA-specific human T cell clones tested to proliferate and secrete IFN-γ and IL-13, LFA-1α L326-345 stimulated ∼10% of these clones to proliferate and a greater percentage to secrete IL-13. (aai.org)
  • A variety of human autoimmune diseases are thought to be mediated by CD4 T cells ( 1 , 2 ). (aai.org)
  • Here, we collected blood samples from a single, naturally infected Holstein cow at three disease progression stages (Stage I: polyclonal stage, Stage II: polyclonal toward oligoclonal stage, Stage III: oligoclonal stage) and successfully visualized the kinetics of clonal expansion of cells carrying BLV integration sites using our BLV proviral DNA-capture sequencing method. (biomedcentral.com)
  • Our results are the first to demonstrate clonal expansion after the massive depletion of cells carrying BLV integration sites in a naturally infected cow. (biomedcentral.com)
  • Between clonal expansion and cell proliferation, the survival of infected cells requires the silencing of viral expression before the immune response. (biomedcentral.com)
  • Consistent with this, iNKT cells from different organs show distinct basal activation, proliferation and clonal expansion. (elifesciences.org)
  • Less than 5% of cattle develop B-cell leukemia/lymphoma after a prolonged latency period [ 1 ]. (biomedcentral.com)
  • Cutaneous T-cell lymphoma (CTCL) (see the image below) is a heterogeneous group of lymphoproliferative disorders characterized by localization of neoplastic T lymphocytes to the skin, with no evidence of extracutaneous disease at the time of diagnosis. (medscape.com)
  • Early patch-stage cutaneous T-cell lymphoma. (medscape.com)
  • Related articles include Cutaneous B-Cell Lymphoma and Cutaneous Pseudolymphoma . (medscape.com)
  • Among the changes to CTCL classification were the addition of primary cutaneous acral CD8 + T-cell lymphoma as a new provisional entity. (medscape.com)
  • TotalSeq™-C antibodies are compatible with 10x Genomics Chromium Single Cell Immune Profiling Solution . (biolegend.com)
  • The model experimentally infected by BLV showed that early infection is characterized by a massive depletion of proviral clones and polyclonal propagation. (biomedcentral.com)
  • Most anatomical compartments, including mucosal surfaces and solid organs, host large populations of tissue-resident lymphocytes which are uniquely placed to provide local networks for immune surveillance and defence against infection ( Fan and Rudensky, 2016 ). (elifesciences.org)
  • The identification of CMV-CTL clones is one method used to assess cellular immunity against CMV. (nature.com)
  • However, these reports have focused on the entire non-specific immune diversity, and not directly on the cellular immunity specific to virus. (nature.com)
  • In the case of the infectious cycle, viruses reach target lymphocytes by cell-to-cell transfer and establish by entry of viral single-stranded RNA, reverse transcription of the viral RNA, integration as a provirus into the host genome, expression of viral proteins, and budding of new virions. (biomedcentral.com)
  • Tissue homeostasis is critically dependent on the function of tissue-resident lymphocytes, including lipid-reactive invariant natural killer T (iNKT) cells. (elifesciences.org)
  • In an alternate model, influenza vaccine peptide-bearing tetramers were able to detect influenza-specific T cells only after PBMC from influenza-vaccinated individuals were expanded in vitro by a 7-day culture with Ag ( 6 ). (aai.org)
  • These cells can participate in inflammatory responses by releasing mediators that attract or activate other cells, contributing to the pathogenesis of allergic and non-allergic diseases. (mdpi.com)
  • This suggests that unique tissue-specific factors (including local lipid antigens, cytokines and/or hormones) may shape the population of iNKT cells resident in those tissues, ultimately regulating local immune responses. (elifesciences.org)
  • For Proteogenomics analysis, the suggested starting amount of this reagent for titration is ≤ 1.0 µg per million cells in 100 µL volume. (biolegend.com)
  • For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. (biolegend.com)
  • Yet, if and how the tissue environment shapes the antigen specificity of iNKT cells remains unknown. (elifesciences.org)
  • By analysing iNKT cells from lymphoid tissues of mice and humans we demonstrate that their T cell receptor (TCR) repertoire is highly diverse and is distinct for cells from various tissues resulting in differential lipid-antigen recognition. (elifesciences.org)
  • iNKT cells have been traditionally defined by the expression of an invariant TCR α-chain (Vα14-Jα18 in mice or Vα24-Jα18 in humans) and their capacity to recognise the glycolipid antigen α-galactosylceramide (αGalCer) presented on CD1d. (elifesciences.org)
  • Despite this prototypical TCR repertoire gene usage, in recent years it has become apparent that there are variations within the iNKT cell repertoire that ultimately impact the antigen recognition capacity and consequently the functional outcomes during an immune response. (elifesciences.org)
  • Within peripheral tissues iNKT cell recent thymic emigrants exhibit a different TCR repertoire than mature cells, suggesting that the iNKT population is shaped after arrival to the periphery. (elifesciences.org)
  • Moreover, the iNKT cell TCR repertoire changes following immunisation and is shaped by age and environmental changes. (elifesciences.org)
  • Since iNKT cell activation can prevent or promote immunopathology in diverse disease contexts, the strict control of peripheral iNKT cell homeostasis is vital to regulate local immunity. (elifesciences.org)
  • To maximize performance, it is strongly recommended that the reagent be titrated for each application, and that you centrifuge the antibody dilution before adding to the cells at 14,000xg at 2 - 8°C for 10 minutes. (biolegend.com)
  • On the other hand, bulk RNA-sequence analyses reveal that gene expression profiles among patients are different according to the cytomegalovirus reactivation pattern, and are associated with cytokine production or cell division. (nature.com)
  • TotalSeq™ reagents are designed to profile protein levels at a single cell level following an optimized protocol similar to the CITE-seq workflow. (biolegend.com)
  • Enrichment analysis for protein localization showed that mainly intracellular and cell-associated interacting proteins were identified. (degruyter.com)
  • A marked Ab and T cell response to Bb outer surface protein A (OspA) often develops during prolonged episodes of arthritis. (aai.org)
  • We have analyzed the Ag-specific CD4 + T cells in treatment-resistant Lyme arthritis, using class II tetramers, and have shown that arthritis-associated Borrelia burgdorferi ( Bb) sensu stricto outer surface protein A (OspA)-specific T cells can be identified with this technology. (aai.org)
  • An individual T-cell has a specific complementarity-determining region 3 (CDR3) of T cell receptor (TCR)-α and -β, which is a result of the recombination of somatic TCR V-(D)-J genes and junction diversity. (nature.com)
  • Respecting the preferentially intracellular localization of sGAG in vesicle-like structures, also the interaction data indicate sGAG-specific modulation of vesicle-based transport processes. (degruyter.com)
  • Recently, MHC tetramer technology has been developed that allows the direct enumeration of Ag-specific T cells ( 3 , 4 ). (aai.org)
  • Although class I tetramers identified large populations of virus-specific CD8 + cells, a different picture has emerged from the use of class II tetramers. (aai.org)
  • However, little information is available on the gene expression profile (GEP) of CMV-CTL clones according to CMV reactivation patterns (and donor CMV serostatus) after allo-HCT. (nature.com)
  • T cell therapy represents an emerging and promising modality for the treatment of both infectious disease and cancer. (biomedcentral.com)
  • In the context of cell therapy trials, the definition of biomarkers can be extended to include a description of parameters of the cell product that are important for product bioactivity. (biomedcentral.com)
  • This review will focus on biomarker studies as they relate to T cell therapy trials, and more specifically: i. (biomedcentral.com)
  • An overview and description of categories and classes of biomarkers that are specifically relevant to T cell therapy trials, and ii. (biomedcentral.com)
  • Insights into future directions and challenges for the appropriate development of biomarkers to evaluate both product bioactivity and treatment efficacy of T cell therapy trials. (biomedcentral.com)
  • Since virions such as BLV, HTLV-1, and HTLV-2 are particularly unstable, viruses are primarily transmitted by the transfer of a cell carrying an integrated provirus. (biomedcentral.com)
  • Beyond the common features shared by all iNKT cells (including their CD1-restriction and innate-like properties), cells found in discrete tissues have distinct phenotypes and functions that critically modulate the outcome of immunity ( Crosby and Kronenberg, 2018 ). (elifesciences.org)
  • Nonetheless, how signals from the tissue environment shape the iNKT cell population to best fit their function in their tissues of residency remains unclear. (elifesciences.org)