• For the researcher who wishes to envision a more accurate picture of the underlying molecular and physical sizes contained in an immobilized affinity resin, it is instructive to calculate how the ligand loading values and binding capacities relate to the number and size of individual molecules. (thermofisher.com)
  • ZFPs custom-designed to detect Shiga toxin in E. coli O157:H7 were immobilized on a cyclic olefin copolymer (COC) chip, which can function as a non-PCR based molecular diagnostic device. (rsc.org)
  • Cell-free expression of OmpA and GFP proteins using beads-immobilized DNA yielded protein bands with the expected molecular sizes of 27 and 30 kDa, respectively. (intimal.edu.my)
  • Interestingly, we discovered that irreversible and multivalent immobilizations that make microbeads stiffer also stabilize the immobilized proteins against the temperature. (rsc.org)
  • This report investigates the mechanism and specificity by which the protein coating enhances gene transfer, and determines if the protein coating targets the vector for internalization by a specific pathway. (pubmedcentralcanada.ca)
  • Pathogen-specific double-stranded DNA was directly detected by using engineered ZFPs immobilized on the COC chip with high specificity, providing a detection limit of 10 fmol of target DNA in a colorimetric assay. (rsc.org)
  • These discoveries provided precious information regarding both the sensitivity and specificity of protein hormones found within blood-based fluids. (wikipedia.org)
  • Cytoskeleton-associated plectin: in situ localization, in vitro reconstitution, and binding to immobilized intermediate filament proteins. (rupress.org)
  • Background Protein microarrays have enormous potential as in vitro diagnostic tools stemming from the ability to miniaturize whilst generating maximum evaluation of diagnostically relevant information from minute amounts of sample. (intimal.edu.my)
  • We observed that the binding of Grb2 to a bivalent ligand, containing two Sos-derived polyproline-sequences immobilized on a SPR sensor, shows unusual kinetic behavior. (gla.ac.uk)
  • Often, the modification of reactive residues necessary for the interaction between the immobilized protein and the matrix compromise the interaction between the protein and its target. (conicet.gov.ar)
  • Conversion to the infectious conformer is particularly associated with major structural rearrangement in the central portion of the protein (residues 90-120), which has an extended flexible structure in the PrP(C) isoform. (sissa.it)
  • The Grb2 adapter protein is involved in the activation of the Ras signaling pathway. (gla.ac.uk)
  • AFS demonstrates that the binding of proteins to the solid matrix of an agarose microbead alters its stiffness. (rsc.org)
  • As a proof of concept, a sequence-enabled reassembly of a TEM-1 β-lactamase system (SEER-LAC) was previously demonstrated to develop zinc finger protein (ZFP) arrays for the detection of a double-stranded bacterial DNA sequence. (rsc.org)
  • The method allows detection of proteins by autoradiography and is simpler than conventional procedures. (pnas.org)
  • In contrast, regions more towards the termini of the protein remain relatively unaltered. (sissa.it)
  • Solid-phase binding to proteolytically degraded vimentin fragments suggested that plectin interacts with the helical rod domain of vimentin, a highly conserved structural element of all intermediate filament proteins. (rupress.org)
  • The protein release kinetics was influenced by charge at physiological pH 7.4. (hindawi.com)
  • By measuring the increase in protein volume of the formed protein-ligand complexes with AFM, the amount of protein having an orientation that allows binding can be estimated. (diva-portal.org)
  • The mechanism of Fc-mediated interaction of eosinophils with immobilized immune complexes. (biologists.org)
  • For example, K48-linked chains target substrates to the 26S proteasome for rapid degradation, while K63-linked chains can control the assembly of various protein complexes. (springer.com)
  • Immobilized Proteins-Advances in Research and Application: 2012 Edition: ScholarlyBrief. (wikipedia.org)
  • The possibility is discussed that this protein is either linked to the receptor for the Fc portion of IgG, or that it is itself the receptor. (biologists.org)
  • It is hypothesized that infectious prions are generated as the cellular form of the prion protein (PrP(C)) undergoes pronounced conformational change under the direction of an infectious PrP(Sc) template. (sissa.it)
  • The method includes cross-linking and hardening the protein mixture and using a mold to form a protein into a desired shape and size. (google.com)