Immobilized cells for uptake. Medical search. Frequent questions

Immobilized metal affinity chromatography (IMAC) is a commonly used protein enrichment technique that employs Nickel-Nitrilotriacetic acid (Ni-NTA) matrices. (researchsquare.com)
Sitka spruce) was partially purified by a combination of lectin affinity and immobilized metal ion affinity chromatography. (who.int)

Ion channels are membrane protein complexes that translocate ions across cell or organelle membranes, underlying a broad range of the most basic physiological processes from nerve and muscle excitability, to membrane potential setting, pH/cell volume regulation, secretion and absorption. (mdanderson.org)
mechanical force itself can deform cells to open sodium channels, allowing ions to enter and leading to action potentials. (justia.com)
The examination of the performance of raw and immobilized S. (Saccharomyces) cerevisiae in the simultaneous abatement of metal ions from wastewater effluent is the focal point of this article. (aucegypt.edu)
1987. The effect of electrically generated silver ions on human cells. (cdc.gov)
A portion of the total oxidase activity had high affinity for immobilized zinc ions and this feature allowed it to be separated from the bulk of oxidase activity. (who.int)

This higher cytotoxic effect can be correlated to the high cellular uptake of porphyrin immobilized, as observed by confocal images. (figshare.com)
Chitosan nanoparticles having application to improve bioavailability, controlled released of loaded drug also increases cellular uptake and targeting to cancer cells, stabilization of proteins and enhanced the affectivity of anti-microbial agents. (sysrevpharm.org)
To investigate the occurrence of the regulatory CSC in microalgae, we considered as organism of study (strain 211/8 k), a single cell, fresh water green algae (reproduces faster (about 6 h) [15,26], with respect to the algal model organism Igf2 [26], giving the opportunity to study cellular metabolic processes in a short span of time. (biopaqc.com)

Binding and uptake into TM cells was receptor-mediated and suppressible with free peptide. (uni-regensburg.de)
This depends, in turn, upon whether specific active peptide sequences in specific proteins are accessible to arriving cells such as neutrophils and macrophages. (nanomedicine.com)

L-glutamate oxidase (L-GLOD) is immobilized over the measuring junctions of the thermopile prior to the immobilization of the cells using Layer-by-layer self-assembly. (latech.edu)
NADPH oxidase 1 (NOX1) gene-mediated production of reactive oxygen species (ROS) could induce migration and invasion of cervical cancer cells. (biomedcentral.com)
and gymnosperms (Ginkgo biloba L.), oxidase activity was associated with cell walls of developing xylem and was enriched in extracts of cell wall-associated glycoproteins. (who.int)
In all species where oxidase activity was detected histochemically, it was expressed in cell walls of lignifying, differentiating xylem cells and was absent from old wood, cambium and phloem, suggesting that oxidases have a conservative role in lignification of tree xylem. (who.int)

Interestingly, however, this immobilization facilitated uptake and clearance of sperm by macrophages, which are known to infiltrate the female reproductive tract (FRT) following semen exposure. (bvsalud.org)
Interestingly, when encapsulated cells were co-cultured with THP-1 macrophages, co-cultures exhibited TNF-α production enhancement. (springer.com)
Upregulation of NOX1 may be associated with infiltration of M2-type macrophages in cervical cancer tissues, and NOX1 promotes malignant features of cervical cancer cells by stimulating ROS production. (biomedcentral.com)

The extracellular matrix glycosaminoglycan hyaluronan (HA) is an abundant component of skin and mesenchymal tissues where it facilitates cell migration during wound healing, inflammation, and embryonic morphogenesis. (ox.ac.uk)
Exosomes, a type of extracellular vesicles, are released by almost all eukaryotic cells, including tumor cells. (biomedcentral.com)
Singh R, Bose A (2020) Understanding the role of phototrophic extracellular electron uptake in terrestrial carbon cycling at the systems level. (wustl.edu)

Like CD44, the LYVE-1 molecule binds both soluble and immobilized HA. (ox.ac.uk)

However, the molecular mechanism of cofilin recruitment to actin networks during dynamic actin-mediated processes in living cells and cofilin's precise in vivo functions have not been determined. (rupress.org)
This chapter compares the physiological and molecular mechanisms of Fe, Zn, Cd, and Pb uptake, mobilization, and accumulation in rice and discusses the progress and strategies for not only increasing Fe/Zn but also decreasing Cd/Zn accumulation in rice. (intechopen.com)
Ion channels have long been key therapeutic targets in disease intervention and pharmaceutical drug development because of their direct involvement in diverse diseases, vulnerability to small molecular modulation, and accessibility for direct activity measurement on cell membranes by patch-clamp recording from whole cell to single molecule levels. (mdanderson.org)
Photodynamic therapy (PDT) is a potential non-invasive approach for application in oncological diseases, based on the activation of a photosensitizer (PS) by light at a specific wavelength in the presence of molecular oxygen to produce reactive oxygen species (ROS) that trigger the death tumor cells. (figshare.com)
He is lecturing Molecular Cell Biology, Biomaterials and Tissue Engineering subjects to the 1st and 2nd cycles of Biomedical Sciences, Biochemistry, Bioengineering, Biotechnology as well as to the Pharmaceutical Sciences MSc. (cienciavitae.pt)
In-depth exploration of landmark and current papers in genetics, molecular and cell biology, with an emphasis on prokaryotes and eukaryotic microbes. (wustl.edu)

J Cell Biol (2007) 178 (7): 1251-1264. (rupress.org)

These small highly conserved actin-binding proteins are essential regulators of actin dynamics in living cells. (rupress.org)
Because of the low abundance of most native ion channel proteins in mammalian cells, the purified native ion channel complexes are commonly contaminated by an overwhelming number and/or amount of non-specifically co-purified proteins, which makes the downstream mass-spectrometric and functional analyses very difficult. (mdanderson.org)
The non-specific adsorption of blood proteins on nanorobot surfaces could lead to clinical difficulties such as thrombosis and unwanted protein-mediated recognition interactions such as cell-nanorobot and nanorobot-nanorobot adhesion (aggregation). (nanomedicine.com)
Cell adhesion, thrombogenicity, foreign body response and other reactions to implanted materials are related to the amount of adsorbed proteins, hence as an implanted object shrinks to smaller sizes (i.e., to micron-scale) the biological signal to local cell populations can increase enormously because the total amount of protein adsorbed on the implant mass is much greater. (nanomedicine.com)
One early strategy to try to accomplish this in implants was to coat the artificial surface with an adsorbed protein, usually bovine serum albumin (BSA) or high-density lipoproteins, to serve as cell adhesion inhibiting proteins that would resist the adsorption of other proteins. (nanomedicine.com)
Biofilms are made up of a community of bacteria immobilized and embedded in an organic polymer matrix composed of polysaccharides and proteins of bacterial origin ( 14 - 16 ). (cdc.gov)

The outcomes revealed that as the initial metal ion concentrations increased, the adsorption capacity improved, while the removal efficiency of S. cerevisiae yeast cells decreased, with the highest uptake obtained at the optimal conditions: pH = 5.0, 2.0 g S. cerevisiae/L, 25 °C, and a contact time of 25 min. (aucegypt.edu)

Various hydrophilic adsorbed coatings have been attached to artificial surfaces to make them more protein-resistant, in effect "passivating" them against protein adsorption and greatly reducing or preventing cell adhesion to biomedical implants [ 754 ]. (nanomedicine.com)
The tumor suppressor protein, TP53 (p53), is critical for cell health and is often deregulated in cancer. (researchsquare.com)
We can exploit the phosphorylation-rich nature of p53 to extract it from the nuclear material of human cancer cells and enrich the native protein through Ni-NTA chromatography. (researchsquare.com)
Here, we provide detailed information for the extraction, purification and enrichment steps of the protein from glioblastoma multiforme (GBM) cells (U87MG line), suitable for downstream EM sample preparation ( Figure 1 ). (researchsquare.com)

In living cells, cofilin is not necessary for actin assembly on endocytic membranes but is recruited to molecularly aged adenosine diphosphate actin filaments and is necessary for their rapid disassembly. (rupress.org)
Furthermore, permeability was found to be highly temperature-dependent in Chinese hamster ovary (CHO) control cells and artificial membranes (parallel artificial membrane permeability assay). (aspetjournals.org)

The bio-sensor is used for real time detection of the L-glutamate (L-glu) dynamics and metabolism for brain tumor cells immobilized in a microfluidic device. (latech.edu)
The brain tumor cells are immobilized over the thermopile covering measuring and reference junctions of the thermopile using a poly-l-lysine coating layer. (latech.edu)
The thermoelectric L-glu sensor measures the heat produced due to the reaction of L-glu released from immobilized brain tumor cells in the presence of L-GLOD. (latech.edu)
The immobilized brain tumor cells are stimulated using potassium chloride (kcl) and ionomycin to study the dynamics of L-glu release from the cells. (latech.edu)
The thermoelectric sensor is also used to measure the heat produced by normal metabolic activity of the brain tumor cells. (latech.edu)
The L-glu sensor detected the release of L-glu from the cultured brain tumor cells after stimulating with 50 mM potassium chloride. (latech.edu)
The heat production of the brain tumor cells metabolism in anaerobic conditions detected by the thermoelectric metabolism sensor is 54 pW per cell. (latech.edu)
Tumor cell-derived exosomes mediate signal transduction between the tumor and surrounding non-tumor cells. (biomedcentral.com)

The fact that our bodies have way more microbial cells than human cells might hit home the most. (wustl.edu)

Phosphoinositide 3-kinases (PI3Ks) are a family of lipid-modifying enzymes that phosphorylate the 3′-OH group of inositol phospholipids and play key roles in physiology ranging from cell growth and metabolism to organismal development. (nature.com)
This two-compartmental model was parameterized to account for nonspecific binding, bidirectional passive diffusion, and active uptake processes based on the physiology of the cells. (aspetjournals.org)

Such coatings typically may include self-assembled monolayers containing surface-immobilized ethylene glycol groups, commonly known as poly(ethylene glycol) (PEG). (nanomedicine.com)

Specific targeting of TM cells could play a critical clinical role by increasing the therapeutic efficacy of nanoparticles, e.g. for nonviral gene delivery. (uni-regensburg.de)
Compared to the integrin-overexpressing U87-MG cell line, the association of RGD-modified nanoparticles with the TM cells was significantly higher. (uni-regensburg.de)
Therefore, the physical and chemical properties of nanoparticles may be relevant to improve the porphyrin photodynamic activity in BC cells. (figshare.com)

Both during normal tissue homeostasis and particularly after tissue injury, HA is mobilized from these sites through lymphatic vessels to the lymph nodes where it is degraded before entering the circulation for rapid uptake by the liver. (ox.ac.uk)
In the liver, the interplay between uptake and export processes influences the drug intracellular concentration and thereby the amount of drug available for metabolic elimination and/or biliary excretion. (aspetjournals.org)

Unlike other mycorrhizal relationships, such as arbuscular mycorrhiza and ericoid mycorrhiza, ectomycorrhizal fungi do not penetrate their host's cell walls. (wikipedia.org)
In the cell walls of fungi, in the exoskeletons of arthropods mostly in crustaceans (e.g,. crabs, lobsters and shrimps), insects, radulae of molluscs and in beaks and internal shells of cephalopods (e.g. octopus and squid). (sysrevpharm.org)
They live on the surface of the epidermal cells of the plant roots forming dense hyphae covering and branch out to the rhizosphere, but never enter the cell walls. (rodaleinstitute.org)

Endomycorrhizas are the only type of mycorrhiza that associate with herbaceous roots (including vegetables), living inside the host's plant root cells, forming densely branched structures called arbuscules (See Photo 1), and thus are known as arbuscular mycorrhizal ( AM ) fungi. (rodaleinstitute.org)
On the other hand, ECM fungi can acquire and immobilize significant amounts of organically bound N and P in the hyphae, representing up to 80% of plant N and P. (rodaleinstitute.org)

Furthermore, the immobilization process maintained the P photoactivity and improved the photophysical properties of PS, minimizing its aggregation in the cell culture medium. (figshare.com)

iv) Production and Characterization of 3D cell culture models aimed for drug screening. (cienciavitae.pt)

The metabolism from the cells is measured by detecting the decrease in the metabolic rate followed by stopping the glucose supply. (latech.edu)
In general, cancer cells are selectively damaged because of their high metabolic and proliferative rates. (msdmanuals.com)

Furthermore, the utilization of unicellular algae as a model system to study enzymes involved in plant nutrition is generally advantageous because the metabolism responds uniformly to nutrient supply that each cell uptakes from the medium. (biopaqc.com)
Experiments were also performed to detect the heat from brain tumor cell metabolism. (latech.edu)

A team led by U.K.-based University of Cambridge has achieved a breakthrough, discovering that simply adding a potassium iodide solution to the perovskite ink can have the dual effects of healing defects that limit the material's efficiency, and immobilizing ion movement, which increases stability. (pv-magazine.com)

The release and uptake of 1.22 mg dL-1 L-glu concentration is detected. (latech.edu)
he biosorption capacity of immobilized biosorbents for Cr (VI) was found to depend on pH, contact time, biosorbent dose and initial concentration of Cr (VI). (omicsonline.org)

To help address specimen-related challenges, here we present a highly effective protocol to isolate native p53 complexes from human cancer cells suitable for cryo-EM analysis. (researchsquare.com)
This simple, but effective protocol may serve as a new means to investigate authentic p53 structures from many different cancer cells. (researchsquare.com)
Radiation cannot destroy cancer cells without destroying some adjacent normal cells. (msdmanuals.com)

Instead they form an entirely intercellular interface known as the Hartig net, consisting of highly branched hyphae forming a latticework between epidermal and cortical root cells. (wikipedia.org)

Jurkat T cells encapsulated with a double layer of PSS-GNR/PAH (PSS-GNR/PAH@Jurkat) showed the highest rate of cell proliferation when compared to 24-h encapsulated cells. (springer.com)

Quantum dots with cyclo(RGDfC) covalently immobilized to their surface effectively targeted cultured TM cells and were rapidly and efficiently endocytosed by binding to alpha(v)beta(3) and alpha(v)beta(5) integrins. (uni-regensburg.de)
Because of the excellent optical properties of GNRs, the combination of surface-modified GNRs, with LBL cell encapsulation, should provide substantial benefits. (springer.com)

The transplantation of cells or tissues has been applied for treating various human diseases (Bhatia et al. (springer.com)

Scientists led by Cambridge University have discovered that adding a simple solution of potassium to the ink solution has the effect of 'healing' defects in metal-halide perovskite films, and immobilizing ion movement. (pv-magazine.com)
Heavy metal uptake in cells is a grave danger to phosphate-rich genetic material and is the cause of acute poisoning, environmental toxicity, and even cancer 9 . (researchsquare.com)

The biological effect of this system was evaluated against bladder cancer (BC) cells with or without light exposition. (figshare.com)
The effect of multiple layers of immobilized L-GLOD layers is studied. (latech.edu)

We have demonstrated encapsulation of human Jurkat T cells with poly(sodium 4-styrenesulfonate) (PSS)-coated gold nanorods (PSS-GNRs). (springer.com)

Class discussions will center on such key discoveries as the chemical nature of genetic material, the genetic code, oxygen producing light-spectrum, cell-cell signaling, transcriptional regulation, the random nature of mutation, and cell cycle regulation. (wustl.edu)

Due to diminished ATP production, the parasite is immobilized and eventually dies. (pharmaxlifesciences.com)
Cell experiment and animal tumor formation experiment were used to evaluate whether exosomal NOX1 stimulating ROS production to promote M2 polarization of TAM in cervical cancer. (biomedcentral.com)
Recently, overexpression of DEAD-box helicase 19A (DDX19A) was reported to increase NOX-1-mediated production of reactive oxygen species (ROS) to induce migration and invasion of cervical squamous cell carcinoma cells. (biomedcentral.com)

After Jurkat T cells were encapsulated with poly(allylamine hydrochloride) (PAH) and/or PSS-GNRs or PSS, most cells survived and could proliferate. (springer.com)

The experiment of storage demonstrated that the immobilized S. cerevisiae was more stable for 8 months than the raw yeast. (aucegypt.edu)

In addition, cell encapsulation has become an attractive approach in biomedical applications. (springer.com)
These data provide promising results, demonstrating the potential use of encapsulated PSS-GNR/PAH@Jurkat to provide a more inert T cell population for immunotherapy application and other biomedical applications. (springer.com)

Equally important, our protocol may be used to study native p53 structures from many different cancer cell lines. (researchsquare.com)

The stimulators increase the release of L-glu from the brain cells, which mimics pathological conditions. (latech.edu)
IMPACT: Our results suggest a need for planning and resource allocation on a local level to increase mammography uptake. (cdc.gov)

BK channels play a variety of physiologically important roles, such as neuronal firing and neurotransmitter release, frequency tuning of auditory hair cells, hormone secretion, and contractile tone of smooth muscles. (mdanderson.org)
In addition to these catalytic roles, PI3KC2α is also required for genome stability by acting as a scaffold at the mitotic spindle during cell division 26 . (nature.com)

It was subsequently patented as an herbicide and later as a general biocide whereby it immobilizes mineral co-factors (Co, Cu, Fe, Mn, Ni, Zn, etc.) essential for enzyme activity and cell function. (biologix.co.nz)

Complexes of Vps34, the sole class III PI3K member, produce PI 3-phosphate (PI(3)P) in the endolysosomal system and during autophagy to regulate vesicle-mediated sorting en route to lysosomes 1 . (nature.com)

Glyphosate chelates and immobilizes essential mineral nutrients that are essential for plant and animal physiological processes. (biologix.co.nz)
Additional processes, such as nonspecific binding and bidirectional passive diffusion between medium and cells need to be assessed separately from active transport to enable proper mechanistic scaling. (aspetjournals.org)

The results of L-glu released from the cells were also compared with the fluorescence-based assay tests. (latech.edu)

Currently, human T cell therapy is of considerable scientific interest. (springer.com)
Here, we propose an innovative technique of single-cell encapsulation of human T cells using polyelectrolytes combined with gold nanorods. (springer.com)

Anatomy30

Organisms10

Chemicals and Drugs153

Analytical, Diagnostic and Therapeutic Techniques and Equipment22

Phenomena and Processes38

Disciplines and Occupations1

Technology, Industry, Agriculture1

Information Science3

Health Care1

Affinity chromatography2

Ions5

Cellular3

Peptide2

Oxidase4

Macrophages3

Extracellular3

Soluble1

Molecular6

Biol1

Proteins6

Yeast cells1

Protein4

Membranes2

Tumor cells8

Microbial cells1

Physiology2

Commonly1

Nanoparticles3

Liver2

Walls3

Fungi2

Aggregation1

Characterization1

Metabolic2

Metabolism2

Increases1

Concentration2

Cancer Cells3

Epidermal1

Proliferation1

Surface2

Tissues1

Metal2

Effect2

Sodium1

Regulation1

Production3

Poly1

Experiment1

Biomedical2

Study1

Increase2

Roles2

Activity1

Complexes1

Processes2

Assay1

Human2