For in vivo test: In vitro lifestyle and embryo freezing After IVF, oocytes encircled with cumulus cells had been placed in fresh new TCM-199 moderate, as well as the embryos had been co-cultured with cumulus cells, as previously defined [21]. (bioskinrevive.com)
Embryos had been moved into freezing moderate straight, and each embryo was packed right into a 0.25 ml plastic straw (Fujihira, Tokyo, Japan). (bioskinrevive.com)
Grade I and II embryos were divided into three groups: slow freezing (n = 22), vitrification (n = 24) and control (n = 33). (gsk-3inhibitors.com)
Embryos were exposed to 10% EG and 10% DMSO for 1 min and 30 s, and then to 20% EG, 20% DMSO and 0.5 M sucrose for 30 s, always at room temperature. (gsk-3inhibitors.com)
Room temperature2
Spike RBD was detected in immersion fixed paraffin-embedded sections of SARS-CoV-2 infected human lung tissue using Mouse Anti-SARS-CoV-2 Spike RBD Monoclonal Antibody (Catalog # MAB105802) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody ( VC001 ). (rndsystems.com)
AFM/STM measurements on Si(111)-(7×7), Ge(111)-c(2×8), and TiO 2 (110) surfaces are presented at room temperature. (go.jp)
Vitro1
To understand the intrinsic capsid disassembly pathway and how it can be modulated, we have developed a single-particle fluorescence microscopy method to follow the real-time uncoating kinetics of authentic HIV capsids in vitro immediately after permeabilizing the viral membrane. (elifesciences.org)
The method is general and can be extended to prepare GUVs of high and low melting lipids and cholesterol with reconstituted transmembrane proteins at physiological temperature or below avoiding potential destruction of the protein. (researchsquare.com)
Cannot survive1
Humans cannot survive prolonged exposure to a wet-bulb temperature above 35 °C (95 °F). Such a temperature used to be thought not to occur on Earth's surface but has been recorded in some parts of the Indus Valley and Persian Gulf. (wikipedia.org)
Environments3
We also introduce currently debated and evaluated freezing parameterizations for application in model environments. (nature.com)
However, effective thermoregulation is reduced in hot, humid environments such as the Red Sea and Persian Gulf (where moderately hot summer temperatures are accompanied by unusually high vapor pressures), tropical environments, and deep mines where the atmosphere can be water-saturated. (wikipedia.org)
Once temperature becomes the dominant method of entrainment used to yoke the circadian rhythms to leptin, some very unique things happen to our biochemistry that normally do not occur in other environments. (jackkruse.com)
Humidity2
Ice nucleates in various ways from aerosol particles, termed ice-nucleating particles, over an extensive temperature and humidity range. (nature.com)
Fig. 1: Homogeneous and heterogeneous ice nucleation pathways superimposed on the ice-water phase diagram as a function of temperature, relative humidity and water activity. (nature.com)
Particles2
Pore condensation freezing in nanometre-sized pores can explain ice formation by particles in the absence of a coverage of liquid water. (nature.com)
The ability of aerosol particles to act as ice nucleating particles (INPs) depends on meteorological factors such as temperature and ice supersaturation (as a function of vertical velocity), as well as on the aerosol type in the layer in which cirrus developed (Kanji et al. (copernicus.org)
Human2
High temperatures pose serious stress for the human body, placing it in great danger of injury or even death. (wikipedia.org)
The core temperature of a human is regulated and stabilized primarily by the hypothalamus, a region of the brain linking the endocrine system to the nervous system, and more specifically by the anterior hypothalamic nucleus and the adjacent preoptic area regions of the hypothalamus. (wikipedia.org)
Heat3
If skin temperature is greater than that of the surroundings, the body can lose heat by radiation and conduction. (wikipedia.org)
This prevents blood from losing heat to the surroundings and also prevents the core temperature dropping further. (wikipedia.org)
The piston is exposed to heat through an incorporated heating coil, which relies on voltage adjustment to control temperature. (khanacademy.org)
Cycles2
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. (rndsystems.com)
When temperature becomes the dominant environmental trigger and not light cycles, leptin induces endothelial nitric oxide synthetase (eNOS) that shuts down the photic effects of VIP on the SCN. (jackkruse.com)
Water5
Advances include the role of interfacial free energy and pressure on ice nucleation rates, mobility regions of water that generate the critical ice nucleus, classical and non-classical pathways of nucleation, the type of ice polymorph that forms, the impact of solutes on freezing and the role of nanopores as surface features promoting ice nucleation. (nature.com)
It can be applied to describe immersionfreezing and reveals a direct relationship to the water structure at the ice-nucleating particle surface or the contact angle, allowing for interpretation of the interaction between the ice-nucleating particle and the critical ice nucleus. (nature.com)
Insemination was performed with an individual straw after thawing by immersion within a 35C38C water-bath. (bioskinrevive.com)
The ratio of microspheres/nanoparticles, the structure of sacrificial scaffolds, freeze-drying and sintering conditions will be optimized for optimal mechanical properties, as well as efficiency of angiogenesis and cell penetration. (funglass.eu)
The results are important for quantitative analysis of chamber experiments conducted at low temperatures, simulating wintertime or under upper tropospheric conditions. (copernicus.org)
Control2
As core temperature varies from the set point, endocrine production initiates control mechanisms to increase or decrease energy production/dissipation as needed to return the temperature toward the set point (see figure). (wikipedia.org)
It monitors this dance between darkness and light, and the seasonal cold and hot temperatures in our environment to help control and monitor our own growth and development. (jackkruse.com)
High1
Our enzyme kinetics data demonstrates high potency and selectivity of the ppFhCL3 for its cognate FhCL3 enzyme, information that could be utilised to design inhibitors of parasite cathepsin L peptidases. (biomedcentral.com)
We experimentally investigated vapor wall loss of n -alkanes in a Teflon chamber across a wide temperature range. (copernicus.org)
Loss1
Increased wall loss was observed at lower temperatures. (copernicus.org)
Onset1
Based on an extensive set of homogeneous freezing experiments at the AIDA chamber with aqueous sulfuric acid aerosol, we provide a new fit line for homogeneous freezing onset conditions of sulfuric acid aerosol focusing on cirrus temperatures. (copernicus.org)
Gases2
Other simple hydride molecules-methane, hydrogen sulfide, ammonia, hydrogen chloride- are all gases at room temperature and pressure-but not "oxygen hydride" H 2 O. There seems to be some extra stickiness binding water molecules together. (nautil.us)
Our equipment is capable of measuring electronic and IR absorption, fluorescence, and phosphorescence in the temperature range of 4 to 500 K for various types of samples: solutions, glasses, polymer films, crystals, powders, gases, cryogenic matrices made of frozen gases. (photoscience.pl)
Apparatus1
The test material did not show any sign of melting below 360 °C, which was the upper temperature limit for the apparatus used. (europa.eu)
Measurements2
Derived MBL IMF kinetics agree with previous ACE-ENA ground site INP measurements. (copernicus.org)
Measurements of transient absorption and emission make it possible to detect short-lived transients and to characterize the kinetics of their formation and decay with time resolution ranging from picoseconds to hours. (photoscience.pl)
Polymer1
Release kinetics may be controlled by varying polymer composition, different types of stimuli, such as electrical, and varying of implant structure, e.g. as coating. (nih.gov)
Liquid3
These living cells can be maintained for long periods of time by freezing them in liquid nitrogen. (nih.gov)
The test was carried out at 20.5 °C using hexane as the immersion liquid. (europa.eu)
In one of these Bridgewater Treatises, published in 1834, the English chemist William Prout asserted that liquid water's expansion close to its freezing point was an example of this divine providence. (nautil.us)
Room2
After thawing to room temperature, this solution is intended for intravenous use only. (nih.gov)
Stability of the test suspensions was confirmed at Bozo Research Center and showed that 50 and 200 mg/mL suspensions of the test substance (vehicle: olive oil) remained stable for 24 hours at room temperature after storage in light-protected containers in a cold dark place (refrigerator) for 7 days. (europa.eu)
Formation2
Homogeneous freezing is a relevant mechanism for the formation of cirrus clouds in the upper troposphere. (copernicus.org)
At low temperatures in the upper troposphere, both inorganic and organic constituents can induce the formation of ice crystals and thereby impact cloud properties and climate. (copernicus.org)
Time1
Time to detection experiments (TTD) based on turbidometry using an automatic Bioscreen C is a useful and straightforward method for estimating microbial growth parameters (lag time (), growth rate () and work to be done (h0)) at constant temperature. (hocl.com)
Important1
In the atmosphere, homogeneous freezing thresholds have important implications on the cirrus cloud occurrence and related cloud radiative effects. (copernicus.org)
Study3
Supporting information is provided in the form of a second study in which the melting temperature of the test material was assessed in accordance with GLP using a method designed to comply with the standardised guideline EU Method A.1. (europa.eu)
In this pilot study, a wrap containing frozen ice packs targeting the carotid arteries at the front of the neck is used to attempt to evaluate the benefits of targeted carotid cooling in the migraine patient. (chillcryo.net)
In addition, the established secondary models in this study have good performances on predicting the effect of recovery temperature on bacterial growth parameters. (hocl.com)
Cold2
A team of astronomers announced this year that they had found no fewer than eight planets orbiting the so-called Goldilocks zone around their parent stars, inside which the temperatures are neither too hot nor too cold to support life as we know it on Earth. (nautil.us)
The test suspensions were prepared at a frequency of at least once every 7 days and stored in a cold dark place (refrigerator, observed temperature: 3°C-5°C) in light-protected containers (brown glass bottles) until use. (europa.eu)
Upper1
The test material was heated to the upper temperature limit for the test, 400 °C, without self-ignition. (europa.eu)
System1
The core temperature of a human is regulated and stabilized primarily by the hypothalamus, a region of the brain linking the endocrine system to the nervous system, and more specifically by the anterior hypothalamic nucleus and the adjacent preoptic area regions of the hypothalamus. (wikipedia.org)
Change1
The use of polarized spectroscopy, both in absorption and emission, provides information about the transition moment directions, about the orientation of molecules in the investigated sample, and about the kinetics of the processes that are accompanied by a change in the transition moment direction. (photoscience.pl)