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  • Alum
  • a , Macrophages were stimulated with 50 ng ml −1 LPS for 18 h and then 500 µg ml −1 Imject alum ('Alum1') or aluminium hydroxide gel ('Alum2') for 8 h. (nih.gov)
  • b , LPS-primed macrophages were stimulated with the indicated amount of Imject alum for 8 h and analysed as in a . c , Unprimed or LPS-primed WT, ASC-deficient, Nalp3-deficient, caspase-1-deficient (Casp1) and Ipaf-deficient macrophages were stimulated with Imject alum (500 µg ml −1 ) for 8 h, and the IL-1β released into the culture supernatants was measured by ELISA. (nih.gov)
  • e , WT or Nalp3-deficient LPS-primed macrophages were stimulated with either Imject alum, CFA (120 µg ml −1 ) or IFA (30 µl ml −1 ) for 8 h and analysed as in a . (nih.gov)
  • antibody
  • Purified and activated forms of ovalbumin (OVA) from egg whites for use as a carrier protein to prepare immunogens for antibody production or secondary target for screening. (thermofisher.com)
  • Furthermore, in vivo, mice deficient in Nalp3, ASC (apoptosis-associated speck-like protein containing a caspase recruitment domain) or caspase-1 failed to mount a significant antibody response to an antigen administered with aluminium adjuvants, whereas the response to complete Freund's adjuvant remained intact. (nih.gov)
  • polypeptide
  • The CCH protein is composed of two very large polypeptide subunits (404/351 kDa) that form an extremely stable heterodidecameric structure even in the absence of divalent cations. (thermofisher.com)
  • molecules
  • Carrier proteins are large, complex molecules capable of stimulating an immune response upon injection. (thermofisher.com)
  • SR proteins carry domains rich in Ser and Arg that are substrates for cellular protein kinases and phosphatases, which in turn may be linked to numerous stimuli through canonical protein kinase cascades or via regulatory molecules such as calmodulin. (plantphysiol.org)
  • After immunizing mice with each of the proteins adsorbed onto aluminum hydroxide as adjuvant, we measured serum IgG responses as a physiological measure of the antigen's ability to be presented on major histocompatibility complex class II molecules and to prime CD4 + T cells in vivo. (rupress.org)
  • surfaces
  • therefore, they occur predominantly on the outside surfaces of native protein tertiary structures where they are readily accessible to conjugation reagents introduced into the aqueous medium. (thermofisher.com)
  • popular
  • However, NHS esters and imidoesters are the most popular amine-specific functional groups that are incorporated into reagents for protein crosslinking and labeling. (thermofisher.com)
  • products
  • The "mc" designation denotes the fact that that all Imject KLH products are purified from KLH that is obtained from sustainable maricultures of limpets rather than from wild-harvested limpets. (thermofisher.com)
  • extent
  • In this article, we report on the use of the Pierce Primary Neuron Isolation Kit method for generating high-yield, high-viability, high-quality neuronal cultures as manifested by their morphological characteristics, cell-specific protein expression, and the extent of synaptic scaling. (thermofisher.com)
  • Expression
  • In this paper the authors have used a gene gun to administer DNA vaccine and evaluate the protective immune responses generated Methods Plasmids T7 expression vector pRSET B was used to express recombinant Bm-ALT-2 as histidine tagged protein. (prezi.com)
  • stable
  • We began by comparing bovine pancreatic ribonuclease (RNase-A), a compact stable protein, with its variant RNase-S, in which a single peptide bond is cleaved (between Ala20 and Ser21) ( 16 , 17 ). (rupress.org)
  • important
  • Similar SR domain-containing proteins have been implicated in alternative splicing in plants ( Reddy, 2004 ), and it is probable that phosphorylation of these proteins is an important aspect of the regulation of the activities of these proteins. (plantphysiol.org)
  • alum
  • Ten Balb/c mice were injected subcutaneously with 4 doses of 15 ug of rBmALT-2 in Imject® alum and at 2 weeks interval and serum was collected for antibodies. (prezi.com)
  • a , Macrophages were stimulated with 50 ng ml −1 LPS for 18 h and then 500 µg ml −1 Imject alum ('Alum1') or aluminium hydroxide gel ('Alum2') for 8 h. (nih.gov)
  • b , LPS-primed macrophages were stimulated with the indicated amount of Imject alum for 8 h and analysed as in a . c , Unprimed or LPS-primed WT, ASC-deficient, Nalp3-deficient, caspase-1-deficient (Casp1) and Ipaf-deficient macrophages were stimulated with Imject alum (500 µg ml −1 ) for 8 h, and the IL-1β released into the culture supernatants was measured by ELISA. (nih.gov)
  • e , WT or Nalp3-deficient LPS-primed macrophages were stimulated with either Imject alum, CFA (120 µg ml −1 ) or IFA (30 µl ml −1 ) for 8 h and analysed as in a . (nih.gov)
  • a, b , LPS-primed or unprimed macrophages from WT mice ( a, b ), Nalp3-deficient mice and caspase-1-deficient (Casp1) mice ( a ) and from ASC-deficient mice ( b ) were stimulated with Imject alum (500 µg ml −1 ) for the indicated durations, and cell lysates were immunoblotted with antibodies against the p10 subunit of caspase-1. (nih.gov)
  • bovine
  • We began by comparing bovine pancreatic ribonuclease (RNase-A), a compact stable protein, with its variant RNase-S, in which a single peptide bond is cleaved (between Ala20 and Ser21) ( 16 , 17 ). (rupress.org)
  • biological
  • Finally, we discuss the potential mechanisms underlying the immunomodulating effects of nanomaterials by describing the involvement of the protein corona in the interaction of nanomaterials with biological components and by presenting recent data about the adjuvant effects of well-characterized particle adjuvant, aluminum salt, as an example of immunomodulatory particulate. (frontiersin.org)
  • contains
  • The guide also contains a discussion on various applications where crosslinking has been applied, including the powerful label-transfer technique for identifying or confirming protein interactions. (thermofisher.com)
  • sites
  • Enhancing lysosomal proteolysis by the presence of protease-specific cleavage sites ( 12 ) or by destabilizing proteins also favored presentation to T cell hybridomas ( 13 - 15 ). (rupress.org)
  • groups
  • However, NHS esters and imidoesters are the most popular amine-specific functional groups that are incorporated into reagents for protein crosslinking and labeling. (thermofisher.com)