Imaging microscopy ultraviolet. Medical search. Frequent questions

Microscopy with UV Surface Excitation (MUSE) is a novel microscopy method that utilizes the shallow penetration of UV photons (230-300 nm) excitation. (wikipedia.org)
With 280 nm deep UV excitation and simple but robust hardware design, MUSE system can collect fluorescence signals without the need for fluorescence filtering techniques or complex mathematical image reconstruction. (wikipedia.org)
Farzad Fereidouni, Ananya Datta Mitra, Stavros Demos, Richard Levenson, "Microscopy with UV Surface Excitation (MUSE) for slide-free histology and pathology imaging," Proc. (wikipedia.org)
We compared the potential of UV-sensitive low-light-level wide-field (UV-WF) imaging with that of multiphoton (MP) excitation microscopy to monitor DHE in living cells. (epfl.ch)
Additionally, when employed with the higher signal levels of conventional fluorescence imaging techniques, the extreme sensitivity of the EMCCD system allows the use of lower fluorophore concentrations and/or lower power levels from the excitation source, thereby reducing both the potential toxicity to living cells and photobleaching of the fluorescent probe. (fsu.edu)
Illustrated in Figure 1 is a self mode-locked Ti:sapphire pulsed laser, which is currently one of the preferred laser excitation sources in a majority of multiphoton fluorescence microscopy investigations. (fsu.edu)
Microscopy with Ultraviolet Surface Excitation, or MUSE, as the novel technology is called, permits the creation of diagnostic-quality images of tissue samples that are generated in minutes using LED light, and eliminates the need for the traditional time-consuming preparation of samples and glass slides. (ucdavis.edu)
In structured illumination microscopy (SIM), the sample is illuminated with a series of highly structured excitation light ( e.g ., a pattern of parallel lines). (edu.hk)
The microscope has been modified at ORNL for advanced imaging techniques including piezoresponse force microscopy (PFM) and band excitation methods. (ornl.gov)
Kratos developed innovations such as the delay-line detector for spectroscopy and imaging modes and high energy, motorised, X-ray excitation sources ensure the AXIS Supra is capable of performing in the most demanding research and development environments. (vacutec.co.za)
We demonstrate the combination of TR-PEEM with optical time-domain spectroscopy to enable time- and excitation frequency-resolved PEEM imaging. (lu.se)
Ultraviolet fluorescence microscopy (UV excitation filter set at 330-365 nm or 450-490 nm) is a sensitive technique for rapidly examining stool sediments for Cyclospora oocysts, which stand out because they autofluoresce ( Cryptosporidium parvum/hominis oocysts do not). (cdc.gov)

see Figure 1), spinning disk confocal in live-cell imaging, flux determinations of calcium or other ions, and time-resolved three-dimensional microscopy (4-D techniques), the electron multiplying CCD offers significant advantages over other sensors designed for low signal levels. (fsu.edu)
These systems are sometimes referred to as proximity-focused image intensifiers and utilize a photocathode closely coupled to a micro-channel plate ( MCP ) electron multiplier. (fsu.edu)
Visual and photographic microscopy in which electron beams with wavelengths thousands of times shorter than visible light are used in place of light, thereby allowing much greater magnification. (genomicglossaries.com)
In high-resolution electron microscopy one can begin to do `` crystallography without crystals'', averaging thousands of images of single molecules or other assemblies to reveal near atomic level structure. (genomicglossaries.com)
Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana- Champaign Molecular Biomedicine in the Era of Teraflop Computing - DDDAS.org Narrower term: transmission electron microscopy (TEM). (genomicglossaries.com)
Microscopy in which the samples are first stained immunocytochemically and then examined using an electron microscope. (genomicglossaries.com)
Electron microscopy studies favor origin from the causative agents, while others have favored elaboration from apocrine sweat. (medscape.com)
In the years that followed, Peter Paul Biro trained in optical and electron microscopy and crystallography, all of which enabled him to study and identify pigments at the molecular level. (peterpaulbiro.net)
The morphologies of the F-GQDs were characterized by transmission electron microscopy (TEM), and X-ray photoelectron spectroscopy (XPS) measurements were performed on ESCALAB using an AI Ka radiation. (hindawi.com)
The second chamber of the system houses equipment for surface analysis including electron spectroscopies (X-ray photoemission, Auger electron, and Ultraviolet photoemission spectroscopies (XPS, AES, UPS)), and low energy electron diffraction (LEED). (ornl.gov)
The major spectroscopic techniques used in industrial hygiene include mass spectrometry (MS), scanning electron microscopy (SEM), X-ray microanalysis (XM), atomic spectrometry (AS), ultraviolet/visible (UV/VIS) photometry, fluorescent spectrometry (FS), Fourier transform infrared (FTIR) spectroscopy, and Raman spectroscopy (RS). (cdc.gov)
While various techniques such as cryogenic-electron microscopy, nuclear magnetic resonance, and x-ray crystallography are capable of providing high-resolution structures of MMAs, they come with some limitations. (maastrichtuniversity.nl)
The patented AXIS technology ensures high electron collection efficiency in spectroscopy mode and low aberrations at high magnifications in parallel imaging mode. (vacutec.co.za)
Schottky field emission scanning Auger microscopy (SAM) and secondary electron microscopy (SEM) and ion scattering spectroscopy (ISS). (vacutec.co.za)
The synthesized nanoparticles were characterized by X-ray diffraction (XRD), ultraviolet (UV), photoluminescence (PL), and scanning electron microscopy (SEM) techniques. (iisc.ac.in)
Time-resolved photoemission electron microscopy (TR-PEEM) belongs to a class of experimental techniques combining the spatial resolution of electron-based microscopy with the time resolution of ultrafast optical spectroscopy. (lu.se)
The changes were quantified through rugosimetric analysis and Scanning Electron Microscopy. (bvsalud.org)
The Scanning Electron Microscopy analyses were performed under magnifications of 250x, 500x, 1000x and 2000x. (bvsalud.org)

Your research will involve lensless imaging experiments with a high-harmonic generation source, and image reconstruction from diffraction data using advanced concepts such as ptychography. (academictransfer.com)
While lens-based imaging is challenging at EUV wavelengths, lensless imaging methods based on coherent diffraction form a versatile and powerful solution. (academictransfer.com)
The best resolution of conventional light microscopy is limited to ~200 nm due to the diffraction of light. (edu.hk)
Super-resolution microscopy overcomes this diffraction limit and allows scientists to observe and study subcellular or even molecular processes in greater detail. (edu.hk)
This structured illumination is able to produce images that carry high frequency information that lies beyond the diffraction limit. (edu.hk)
Despite its revolutionary impact on biology, all traditional forms of fluorescence microscopy (including widefield, confocal, and multiphoton) face a resolution limit imposed by the diffraction of light through lenses and circular apertures. (fsu.edu)
Due to the fact that most subcellular structures (such as actin fibers, intermediate filaments, microtubules, ribosomes, and transport vesicles) exhibit features much smaller than this size, a mechanism for breaking through the diffraction barrier, and imaging beneath the size limitation that it defines, has been the holy grail of optical microscopy for centuries. (fsu.edu)
The high-resolution TEM (HRTEM) images (Figures 1(b) and S2) indicate the high crystallinity of both GQDs and F-GQDs with a lattice of 0.209 nm, which is consistent with the (102) diffraction plane of sp 2 graphitic carbon [ 21 , 22 ]. (hindawi.com)

A type of scanning probe microscopy in which a probe systematically rides across the surface of a sample being scanned in a raster pattern. (genomicglossaries.com)

In addition, lasers are also the most common light source for scanning confocal fluorescence microscopy, and have been utilized, although less frequently, in conventional widefield fluorescence investigations. (fsu.edu)
Widefield and confocal fluorescence microscopy are capable of magnifying and imaging light-emitting fluorophores with a resolution that approaches a quarter of a micrometer, thus enabling the study of dynamic events and the fine structural details of cellular architecture. (fsu.edu)

We present a setup for generating tunable broadband ultraviolet (UV) laser pulses via achromatic second harmonic generation. (lu.se)
Difference frequency generation (DFG) x-correlation and DFG x-FROG: used to characterize deep ultraviolet (UV) pulses (200-300 nm central wavelength) of the duration longer than ~12 fs. (lu.se)

A new satellite called XRISM (X-ray Imaging and Spectroscopy Mission, pronounced "crism") aims to pry apart high-energy light into the equivalent of an X-ray rainbow. (phys.org)
This reconstruction is based on studies of the extensive color preserved on a replica found in Delos, which has been examined in ultraviolet, infrared, and raking light as well as with X-ray fluorescence spectroscopy and ultraviolet-visible absorption spectroscopy. (metmuseum.org)
Diffusion coefficients of 0.25-μm-diameter endocytic vesicles containing DHE were determined by MP spatiotemporal image correlation spectroscopy. (epfl.ch)
New compact extreme ultraviolet (EUV) radiation sources with wavelengths from roughly 10-100nm are producing numerous emerging EUV applications including high resolution imaging with resolutions down to 0.5nm, molecular and solid-state dynamics research, optical and photoelectron spectroscopy, and nano-machining for nanotechnology. (edmundoptics.co.uk)
Ultraviolet-visible spectroscopy was characterized with an UV/VIS/NIR spectrometer. (hindawi.com)
Disturbances in energy-producing pathways in heart muscle contribute to heart conditions as Hyperpolarised magnetic resonance spectroscopy, a novel imaging technology available only in a few centres worldwide, allows the measurement of these metabolic pathways, potentially leading to new treatments for heart disease. (opengovasia.com)
With market leading performance in both imaging and spectroscopy modes the AXIS Supra combines the highest level of automated sample handing with flexibility to incorporate complimentary analytical techniques in the analysis chamber. (vacutec.co.za)

Peter Paul Biro is also an accomplished optical microscopist with over 30 years of experience in identifying painting materials, principally through polarized light microscopy. (peterpaulbiro.net)

Label-free and slide-free histology via ultraviolet photoacoustic microscopy may allow for rapid diagnoses of bone-tissue pathologies and aid the intraoperative determination of tumour margins. (thefatimafoundation.com)

The lasers commonly employed in optical microscopy are high-intensity monochromatic light sources, which are useful as tools for a variety of techniques including optical trapping, lifetime imaging studies, photobleaching recovery, and total internal reflection fluorescence. (fsu.edu)
Presented in Figure 1 is an example of superresolution imaging using total internal reflection fluorescence ( TIRF ) microscopy. (fsu.edu)

The intensified CCD ( ICCD ) camera system is presently among the most commonly employed imaging methods for low-light techniques such as time-resolved fluorescence experiments, ratio imaging of ion-sensitive fluorochromes, single molecule fluorescence, and other dynamic studies in living cells. (fsu.edu)
Stochastic optical reconstruction microscopy (STORM) is a widely-used single-molecule localisation microscopy technique. (edu.hk)

Techniques based on this strategy are often referred to as probe-based superresolution, and are commonly referred to by names photoactivated localization microscopy ( PALM ), fluorescence photoactivation localization microscopy ( FPALM ), and stochastic optical reconstruction microscopy ( STORM ). (fsu.edu)

Near-ultraviolet light of certain wavelengths can damage layers of the eye near the surface, and can contribute to cataract formation in the lens, especially in younger persons, whose eye tissues have greater transparency in this wavelength range. (microscopyu.com)
Fluorescence microscopy, a fundamental tool of modern biology, achieves this by applying dyes that react with the targeted molecules to give off specific wavelengths of light when exposed to ultraviolet radiation. (morgan.edu)

The following discussion of laser beam characteristics is presented as a general overview of the subject that may prove useful in employing lasers in microscope imaging, laser trapping, and other applications. (fsu.edu)
Lasers have become integral components of many current optical microscopy techniques, and when combined with complex optical systems, they can constitute a significant hazard if safe procedures are not strictly followed. (microscopyu.com)
You have a PhD-degree in Physics or Technical/Applied Physics (or a similar subject), with experience in any (or multiple) of the following subjects: high-harmonic generation, ptychography, coherent imaging, ultrafast lasers,, and experiments involving EUV radiation. (academictransfer.com)
Lasers operate in the infrared, visible, ultraviolet, or X-ray regions of the spectrum. (genomicglossaries.com)

A new purpose-built microscope has been developed that uses the complex properties of UV light to improve image resolution and contrast. (phys.org)
It has potential for generate high quality images containing more information than microscope slides in terms of its 2.5 dimensional features. (wikipedia.org)
The most common methods include temporally scrambling laser light by rapidly varying the optical path length between the light source and the microscope, or scanning the specimen point by point as is the case in confocal microscopy systems. (fsu.edu)
Four particular top trends highlighted in 2018 were open-source DIY microscopy, miniaturized microscope objectives, liquid lenses in imaging, and extreme ultraviolet (EUV) optics. (edmundoptics.co.uk)
Miniaturized, compact microscope objectives reduce the size and weight of microscopy systems, making them more portable. (edmundoptics.co.uk)
In addition to serving as a standalone confocal or super-resolution microscope, the Nikon N-SIM/N-STORM/A1 system also allows researchers to perform correlative confocal-super-resolution imaging. (edu.hk)
The third chamber adds an Omicron variable temperature scanning tunneling/atomic force microscope (STM/AFM) for atomically resolved images of surfaces. (ornl.gov)
In this work, we design three simple yet useful filters based on the combinations of mathematical functions, which are proved to be effective in strengthening the images acquired from the fluorescence microscope. (bvsalud.org)
The sin of the angle µ , therefore, has a maximum value of 1.0 (sin(90°) = 1), which is the theoretical maximum numerical aperture of a lens operating with air as the imaging medium (using 'dry' microscope objectives). (pooher.cn)
Microscope objectives are now available that allow imaging in alternative media such as water (refractive index = 1.33), glycerin (refractive index = 1.47), and immersion oil (refractive index = 1.51). (pooher.cn)
A microscope is an optical device used to image an object onto the human eye or a video device. (edmundoptics.com)
Bruker's Multi-View Selective-Plane Illumination Microscope (SPIM) is not only the fastest light-sheet system on the market, but also incorporates years of Luxendo imaging experience and innovation. (bruker.com)
We validate the technique with gold-standard haematoxylin-and-eosin histology images acquired via a traditional optical microscope, and also show that an unsupervised generative adversarial network can virtually stain the ultraviolet-photoacoustic-microscopy images, allowing pathologists to readily identify cancerous features. (thefatimafoundation.com)
Cyclospora oocysts also are autofluorescent, meaning that when stool containing the parasite is viewed under an ultraviolet (UV) fluorescence microscope the oocysts appear blue or green (see image above) against a black background. (cdc.gov)

The coherence of monochromatic light produced by most laser systems introduces problems in the application of these light sources for classical widefield microscopy. (fsu.edu)

Dehydroergosterol (DHE) is an intrinsically fluorescent sterol with absorption/emission in the ultraviolet (UV) region and biophysical properties similar to those of cholesterol. (epfl.ch)
The requirement of extremely high laser power for visualization of DHE by MP microscopy made this method less potent for multicolor applications with organelle markers like green fluorescent protein-tagged proteins. (epfl.ch)
The project aims to develop nano-scale fluorescent probes for rapid imaging of cells at the molecular level. (morgan.edu)
Dr. Abebe's project will develop new fluorescent dyes or "probes" that can be used to rapidly produce precise images of the targeted metal ions making it possible to identify, visualize and quantify various metal ions in living cells. (morgan.edu)
Microscopy of specimens stained with fluorescent dye (usually fluorescein isothiocyanate) or of naturally fluorescent materials, which emit light when exposed to ultraviolet or blue light. (genomicglossaries.com)
Immunofluorescence microscopy utilizes antibodies that are labeled with fluorescent dye. (genomicglossaries.com)
This is particularly useful when one needs to perform multi-colour imaging, and when not all fluorescent labels are compatible with super-resolution microscopy. (edu.hk)
Central to the recent dramatic rise in the use of fluorescence microscopy in cell biology has been the development of genetically-encoded fluorescent proteins that act as endogenous labels to enable virtually any protein or peptide to become a fluorescent homing beacon for imaging and analysis. (fsu.edu)
Figure 1(a) is the pixelated image of fluorescent protein single molecules attached to a coverslip. (fsu.edu)

The main significance of MUSE system comes from its capacity to produce high-resolution microscopic image with subcellular features in a time-efficient manner with less costs and less lab-expertises requirements. (wikipedia.org)
Our understanding of the biological processes of living cells and how they interact with their environment depends in many ways on our ability to produce detailed and precise images of them at microscopic scales. (morgan.edu)
A method to provide microscopic images of living tissue by analyzing the reflection data of high-frequency ultrasound. (bvsalud.org)

As a postdoc you will develop new methods for high-resolution of nanoscale objects using extreme ultraviolet light. (academictransfer.com)

By integrating microscopy and fresh tissue fluorescence staining into an automated optical system, the overall acquiring time needed for getting digital images with diagnostic values can be much shortened into the scale of minutes comparing with conventional pathology, where general procedure can take from hours to days. (wikipedia.org)
The inherent advantages of scientific charge-coupled device ( CCD ) sensors for digital imaging in optical microscopy have made them ubiquitous in a wide variety of applications. (fsu.edu)
Here, we report a transparent, flexible neural electrode technology based on graphene, which enables simultaneous optical imaging and electrophysiological recording. (nature.com)
Simultaneous electrophysiology and optical imaging could leverage the temporal and spatial resolution advantages of both techniques. (nature.com)
Avantier produces high quality optical components (e.g. precision optical filters, prisms, beamsplitters, high performance camera lenses) that deliver high image quality and improve the accuracy and reliability of the tracking systems used in AR/MR/VR applications. (laserfocusworld.com)
A polymer-only ultraviolet imprint process potentially saves costs, simplifies the process, and increases the reliability of the optical element. (laserfocusworld.com)

Plants that glow under ultraviolet (UV) light aren't just inventions of science fiction TV and movies. (phys.org)
In ultraviolet light, bands of wave and ray patterns can be seen on the mantle. (metmuseum.org)
The entire signal collected is the desired light, and all photons collected contribute to the image formation. (wikipedia.org)
The LED's light is focused using a ball lens with a short focal length onto the sample surface in an oblique-angle cis-illumination scheme since standard microscopy optics do not transmit UV light efficiently. (wikipedia.org)
One of the few significant shortcomings of conventional high-performance CCD cameras is that very low signal levels typically fall beneath the read noise floor of the sensor, limiting the imaging capabilities in a number of currently productive research areas that demand rapid frame-rate capture at extremely low light levels. (fsu.edu)
By elevating photon-generated charge above the read noise of the device, even at high frame rates, the EMCCD has the capability of meeting the needs of ultra-low-light imaging applications without the use of external image intensifiers. (fsu.edu)
The performance of all CCD-based detectors has improved dramatically in recent years, and increased sensitivity has lowered detection limits in high-performance low-light imaging systems significantly. (fsu.edu)
Furthermore, when imaging is performed at video frame rates and faster, the read noise increases to an unacceptable level, relative to the signal, in low-light conditions. (fsu.edu)
A successful technique employed by some investigators to improve differential interference contrast ( DIC ) images produced with an argon ion laser light source is to position a circular glass wedge, spinning at 2500 revolutions per minute, in the light path. (fsu.edu)
We demonstrate that hippocampal slices can be imaged through transparent graphene electrodes by both confocal and two-photon microscopy without causing any light-induced artefacts in the electrical recordings. (nature.com)
Light in the near-infrared can produce surface damage as well, although at a higher damage threshold than for ultraviolet light. (microscopyu.com)
Ultraviolet (UV) light is one of the most harmful environmental factors that contribute to skin damage. (spandidos-publications.com)
Exposure to ultraviolet (UV) light, particularly UV-B (290-320 nm), is a major etiological factor that contributes to skin damage ( 1 ). (spandidos-publications.com)
Leonardo da Vinci: A Closer Look reexamines those works in the light of the latest analytical technologies, including microscopy, ultraviolet imaging, infrared reflectography and X-ray fluorescence (XRF). (thehistoryblog.com)
PCO's high-speed cameras offer best light sensitivity and furthermore real 10 to 12 bit dynamic range with leading image and color quality for your applications. (vision-systems.com)
In addition, these filters can be used to enhance the low-light image, which provide satisfactory visual information and marginal profile for the blurred objects in the image. (bvsalud.org)
Image-forming light waves pass through the specimen and enter the objective in an inverted cone as illustrated in Figure 1. (pooher.cn)
As the light cones change, the angle µ increases from 7° in Figure 2(a) to 60° in Figure 2(c), with a resulting increase in the numerical aperture from 0.12 to 0.87, nearing the limit when air is the imaging medium. (pooher.cn)
This is important because it ensures the user is not imaging the filament of the light bulb. (edmundoptics.com)
The end result is an image of relative contrast between parts of the object and the light source. (edmundoptics.com)
When imaging, obstacles like pigments or cell nuclei can interfere with light, leading to images with shadows and striping artifacts. (bruker.com)
When imaging thicker samples or opaque specimens, light scattering can lead to reduced contrast and image blur. (bruker.com)
The key is the use of solutions of water and a special type of oil that polymerizes solidifies when exposed to ultraviolet light. (scitizen.com)

The optics and imaging industries are constantly evolving because of new developments driven by markets such as advanced medical diagnostics, machine vision, and semiconductor processing. (edmundoptics.co.uk)
Edmund Optics ® highlights key optics and imaging trends in this monthly Trending in Optics series to keep the community informed of the innovative technologies that are making the largest impact. (edmundoptics.co.uk)
We offer borescopes, fiberoscopes, fiber optic cables, LED illuminators for endoscopy, quartz and polymer imaging guides, video-endoscopes, fiber optics elements made of multi-component glasses, high-purity polymers, quartz and transparent materials in the infrared and ultraviolet range. (endoskopy-techniczne.pl)

The ability to perform high-resolution imaging with extreme ultraviolet (EUV) radiation provides exciting possibilities in science and technology. (academictransfer.com)
In this project we use coherent EUV radiation from a high-harmonic generation (HHG) source, combined with coherent imaging techniques such as ptychography, to perform high-resolution imaging on semiconductor nanostructures. (academictransfer.com)
This glass exhibits excellent transmission in the ultraviolet and visible regions while efficiently blocking infrared radiation. (uqgoptics.com)

The system is capable to produce images from various tissue type in different sizes, either fresh or fixed. (wikipedia.org)
Here, we show that real-time three-dimensional contour-scanning of tissue via ultraviolet photoacoustic microscopy in reflection mode can be used to intraoperatively evaluate undecalcified and decalcified thick bone specimens, without the need for tissue sectioning. (thefatimafoundation.com)

Levenson and his team have created an innovative new method for spectral imaging that drastically reduces the amount of data needed for analysis. (ucdavis.edu)
The VUV regions are surrounded by "above" the ultraviolet (UV) spectral region (from 200 nm) and the long wavelength side of the Hard X-Ray region below (around 1 nm). (horiba.com)

This approach relies on the stochastic activation of fluorescence to intermittently photoswitch individual photoactivatable molecules to a bright state, which are then imaged and photobleached. (fsu.edu)

The color-mapping techniques that correlated fluorescence staining to traditional H&E staining provide the same visual representation to pathologists based on existing knowledge with no need for additional training on image recognition. (wikipedia.org)
Although microscopy techniques used for calcium imaging have generally a small field of view, complete transparency over larger areas is critical to use them with wide-field voltage imaging or in serial calcium imaging experiments to correlate small-scale and large-scale dynamic networks. (nature.com)
As of yet, neural recordings with completely transparent, flexible electrode arrays, compatible with in vitro and in vivo imaging techniques, have not been demonstrated, to our knowledge. (nature.com)
As imaging technologies have revolutionized research in many areas of biology and medicine, neuroscientists have often pioneered the use of new visualization techniques. (cshlpress.com)
Imaging in Neuroscience: A Laboratory Manual is an essential guide to discovering and implementing these techniques. (cshlpress.com)
Addressing topics such as chromatin immunoprecipitation, topological studies, photocrosslinking, FRET and imaging techniques, the volume fully updates and expands upon the successful previous editions.Written in the convenient and informative 'Methods in Molecular Biology' series format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. (nhbs.com)
For that, aspects of soft-ionizing techniques (nano-electrospray ionization and matrix-assisted laser desorption/ionization), axial/orthogonal reflectron time-of-flight and high-resolution Orbitrap MS, top-down proteomics (ultraviolet photodissociation and higher-energy collisional dissociation), and mass-to-charge ratio/time-resolved imaging using pixelated Timepix and Timepix3 application-specific integrated circuit based detection assemblies were brought together. (maastrichtuniversity.nl)
Thebook will teach you about:Neural networks, a beautiful biologically-inspired programmingparadigm which enables a computer to learn from observational dataDeep learning, a powerful set of techniques for learning in neuralnetworksNeural networks and deep learning currently provide the best solutionsto many problems in image recognition, speech recognition, and naturallanguage processing. (thefatimafoundation.com)

MuVi SPIM allows for high-speed volumetric acquisition of dynamic processes in live specimens, as well as cleared sample imaging with compatibility with any clearing method. (bruker.com)
Ultraviolet fluorescence microscopy is one method of examining stool specimens for Cyclospora oocysts. (cdc.gov)

You will join an active research team, working on advancing HHG source technology, optimizing HHG-based imaging, developing new ptychography algorithms, and applications towards imaging of nanostructures. (academictransfer.com)

Examples of this type of superresolution imaging are stimulated emission depletion ( STED ), ground state depletion ( GSD ), and saturated structured illumination ( SSIM ) microscopy. (fsu.edu)

In addition, because the charge packet from a single pixel is often only a few electrons in challenging microscopy investigations, the signal is too frequently lost in the read noise even at slow readout rates. (fsu.edu)
One proven solution to the read noise limitation when higher frame rates are required has traditionally been to employ an image intensifier to multiply the number of emitted specimen photons prior to detection and readout by a conventional CCD. (fsu.edu)
During image acquisition, the activation laser stochastically activates a sparse set of probes, which are then imaged with the readout laser. (edu.hk)

We found that the lateral resolution of MP microscopy is ∼1.5-fold higher than that of UV-WF deconvolution microscopy, allowing for improved spatiotemporal analysis of plasma membrane sterol distribution. (epfl.ch)
The encoded information can subsequently be reconstructed using special algorithms as super-resolution images with a lateral resolution of up to 115 nm. (edu.hk)
This process is repeated for thousands of times to obtain enough localisation information to reconstruct a super-resolution image with a lateral resolution of up to 20 nm. (edu.hk)

His expertise in digital imaging, hyperspectral imaging and digital image processing has helped resolve numerous attribution issues. (peterpaulbiro.net)

A specific type of backlight illumination used in microscopy applications is Koehler illumination. (edmundoptics.com)
It is designed to provide bright and even illumination on the object plane and on the image plane where the image produced from the objective is then reimaged through the eyepiece. (edmundoptics.com)

Following in the footsteps of the Neptune image released in 2022, the NASA/ESA/CSA James Webb Space Telescope has taken a stunning image of the solar system's other ice giant, the planet Uranus. (phys.org)

Sections focus on imaging at the molecular level, axons and nerve terminals, spines and dendrites, neurons and circuits in vitro, neurons and circuits in vivo, glia, brain dynamics and behavior, and brain pathology. (cshlpress.com)
The development of new probe technologies, such as quantum dots and high-resolution laser fluorescence microscopy, allow real- time observations of molecular interactions and trafficking within living cells. (genomicglossaries.com)

It finds use in applications such as fluorescence microscopy, photobiology, and UV imaging. (uqgoptics.com)
Liquid lenses can be integrated into imaging systems to quickly adjust the focus to accommodate objects located at different heights or working distances in high-speed applications. (edmundoptics.co.uk)
Traditional imaging lenses in applications that require a quick refocus struggle to capture sharp and accurate images because they require mechanical adjustment. (edmundoptics.co.uk)
High sensitivity, excellent energy resolution and fast, high resolution imaging meet the analysis needs of the most demanding applications. (vacutec.co.za)

If suspect Cyclospora oocysts are found, bright-field, phase contrast, or differential interference contrast microscopy can then be used to confirm that the structures have the characteristic morphologic features of Cyclospora oocysts (i.e., are nonrefractile spheres that contain undifferentiated cytoplasm or refractile globules). (cdc.gov)

Alternatively, researchers can choose to use confocal to scan a larger FOV to provide an overview of the sample, and use super-resolution to image a selected area of interest to examine the area in greater detail. (edu.hk)

Imaging in Neuroscience: A Laboratory Manual provides the definitive collection of methods in use in this groundbreaking field. (cshlpress.com)

Based on special CCD technology the pco.ultraviolet provides highest sensitivity in the UV range of the spectrum and a dynamic range of 14 Bit. (vision-systems.com)

MUSE images have been validated with diagnostic values. (wikipedia.org)
Renal artery with MUSE microscopy. (ucdavis.edu)

Calcium imaging is a versatile experimental approach capable of resolving single neurons with single-cell spatial resolution in the brain. (nature.com)

If the path length or coherence state of the laser beam fluctuates at a faster interval than the detector integration time (in effect, the video frame rate), the speckle and scattering artifacts disappear from the image. (fsu.edu)
Left: Image collected without the destriping module showing striping artifacts. (bruker.com)
Center: Image collected with the destriping module showing successful removal of striping artifacts. (bruker.com)

It is caused by gadolinium exposure used in imaging in patients who have renal insufficiency. (medscape.com)
Nephrogenic systemic fibrosis almost always occurs in patients with renal insufficiency who have had imaging studies (eg, magnetic resonance angiography) with gadolinium, a contrast agent used in imaging studies. (medscape.com)
The pathophysiology of nephrogenic systemic fibrosis is related to the exposure of patients with renal insufficiency to gadolinium in association with imaging studies. (medscape.com)
[ 14 ] As such, gadoversetamide (OptiMARK) and gadopentetate dimeglumine (Magnevist) should not be used for imaging in patients with renal impairment. (medscape.com)

The signal-to-noise ratio obtainable by UV-WF imaging could be significantly improved by pixelwise bleach rate fitting and calculation of an amplitude image from the decay model and by frame averaging after pixelwise bleaching correction of the image stacks. (epfl.ch)
The pco.flim camera system is the first luminescence decay time microscopy camera using a two tap CMOS image sensor. (vision-systems.com)

Significantly reduced photobleaching in MP microscopy of DHE enabled us to acquire three-dimensional z -stacks of DHE-stained cells and to obtain high-resolution maps of DHE in surface ruffles, nanotubes, and the apical membrane of epithelial cells. (epfl.ch)
As a postdoc, your research will be aimed towards developing efficient high-resolution imaging with broadband HHG sources. (academictransfer.com)
Built on Bruker's history of innovation, our next-generation MuVi SPIM incorporates several new features to enhance image quality, shorten acquisition speed, and allow high-resolution and artifact-free imaging of a broader range of samples. (bruker.com)

Anatomy30

Organisms14

Diseases18

Chemicals and Drugs55

Analytical, Diagnostic and Therapeutic Techniques and Equipment75

Psychiatry and Psychology1

Phenomena and Processes60

Disciplines and Occupations10

Anthropology, Education, Sociology and Social Phenomena1

Technology, Industry, Agriculture9

Information Science7

Health Care12

Excitation12

Electron18

Diffraction8

Type of scanning1

Confocal fluorescence microscopy2

Pulses2

Spectroscopy7

Polarized light microscopy1

Slide-free histology1

Total internal ref2

Single-molecule2

Localization Microscopy1

Wavelengths2

Lasers4

Microscope14

Widefield1

Fluorescent9

Microscopic3

Extreme ultraviolet light1

Optical6

Light23

Optics3

Radiation3

Tissue2

Spectral2

Stochastic1

Techniques8

Specimens2

Ptychography1

STED1

Readout3

Lateral resolution3

Digital Imaging1

Illumination2

20221

Molecular2

Applications4

Differential1

Researchers1

Methods1

Sensitivity1

MUSE2

Spatial resolution1

Artifacts3

Renal4

Decay2

High-resolution3