• ALS (Acid Labile Subunit) is a liver-derived protein that exists in a ternary complex with Insulin-like Growth Factor (IGF)-binding Protein-3 (IGFBP-3) or IGFBP-5, and either IGF-I or IGF-II. (rndsystems.com)
  • We determined the presence of IGF-I, -II, and binding protein 5 (IGFBP-5) gene expression in human skeletal muscle and that mRNA abundance is not altered by nutrients and insulin. (diva-portal.org)
  • The Eagle Bioscience's Rat Insulin-like Growth Factor Binding Protein-5 (IGFBP-5) is for research use only and not to be used for diagnostic procedures. (eaglebio.com)
  • Rat insulin-like growth factor binding protein-5 (IGFBP-5) is a 252 amino acid protein which preferentially binds IGF-II compared to IGF-I. It is highly conserved with a single amino acid difference [Asp187] compared to mouse IGFBP-5 [Glu187] and approximately 97% sequence homology to human IGFBP-5. (eaglebio.com)
  • The present study evaluated the ability of IGF-I complexed with IGF-binding protein (IGFBP)-3 to modulate the alcohol-induced inhibition of muscle protein synthesis in gastrocnemius. (psu.edu)
  • We have previously shown that insulin-like growth factor-binding protein-5 (IGFBP-5) is overexpressed in fibrotic lung tissues and that it induces production of extracellular matrix components such as collagen and fibronectin both in vitro and in vivo. (elsevierpure.com)
  • Background: Previous studies have reported inconsistent results regarding the implications of deranged insulin-like growth factor 1 (IGF-1)/insulin-like growth factor-binding protein 1 (IGFBP-1) axis in patients with heart failure (HF). (kent.ac.uk)
  • We conducted a comprehensive analysis, utilizing a resequencing and tagging single-nucleotide polymorphism (SNP) approach, between common genetic variation in the IGF1, IGF binding protein (BP) 1, and IGFBP3 genes with IGF-I and IGFBP-3 blood levels, and prostate cancer (PCa) risk, among Caucasians in the NCI Breast and Prostate Cancer Cohort Consortium. (ox.ac.uk)
  • The growth hormone/insulin-like growth factor (IGF) axis can be manipulated in animal models to promote longevity, and IGF-related proteins including IGF-I and IGF-binding protein-3 (IGFBP-3) have also been implicated in risk of human diseases including cardiovascular diseases, diabetes, and cancer. (ox.ac.uk)
  • Proteolysis of insulin-like growth factor binding protein-1 (IGFBP 1) in pregnancy.J Perinat Med. (mdx.ac.uk)
  • Once IGF1 is bound to Insulin-like Growth Binding Protein (IGFBP), IGF-1 cannot be linked to IGF1R any longer. (proteopedia.org)
  • The major serum IGF-binding protein is insulinlike growth factor binding protein-3 (IGFBP-3). (medscape.com)
  • Insulin growth factor binding protein 5 (IGFBP-5), insulin growth factor (IGF-1) and transforming growth factor (TGF-beta1) in the type II alveolar epithelial cells (AECs) play a key role in lung injury caused by bleomycin and pioglitazone attenuates the lung injury/fibrosis by restoring IGFBP-5 and IGF-1 and decreasing TGF-beta1 expressions in the type II AECs. (genscript.com)
  • Relationship between growth velocity and change of levels of insulin-like growth factor-1, insulin-like growth factor binding protein-3 and, IGFBP-3 promoter polymorphism during GnRH agonist treatment. (cdc.gov)
  • The superfamily of insulin-like growth factor (IGF) binding proteins include the six high-affinity IGF binding proteins (IGFBP) and at least four additional low-affinity binding proteins referred to as IGFBP related proteins (IGFBP-rP). (rndsystems.com)
  • All IGFBP superfamily members are cysteine-rich proteins with conserved cysteine residues, which are clustered in the amino- and carboxy-terminal thirds of the molecule. (rndsystems.com)
  • Post-translational modifications of IGFBP, including glycosylation, phosphorylation and proteolysis, have been shown to modify the affinities of the binding proteins to IGF. (rndsystems.com)
  • Circulating IGF-I and -II are bound to specific insulin-like growth factor (IGF)-binding proteins (IGFBPs), of which IGFBP-3 binds the majority of the IGFs. (nih.gov)
  • Effects of IGFBP-2 on the organism can be compensated through the modified expression of other IGF Binding proteins. (eaglebio.com)
  • Circulating concentrations of insulin-like growth factor I (IGF-I) and IGF binding proteins (IGFBP) have been associated with the risk of several types of cancer. (ox.ac.uk)
  • This review summarizes evidence for the role of proteolytic enzymes that degrade and inactivate insulin-like growth factor binding proteins (IGFBP) during follicular development in mammals. (bioone.org)
  • This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human IGFBP-4. (rndsystems.com)
  • Furthermore, monocytes treated with recombinant IGFBP-5 expressed the mesenchymal markers a-smoothmuscle actin and fibronectin in vitro and in vivo, suggesting that IGFBP-5 can induce the transformation of monocytes into mesenchymal cells. (elsevierpure.com)
  • This study evaluates the roles of IGF1/IGFBP-1 axis in patients with HF with reduced ejection fraction (HFrEF), mid-range ejection fraction (HFmrEF), or preserved ejection fraction (HFpEF). (kent.ac.uk)
  • A comprehensive analysis of common IGF1, IGFBP1 and IGFBP3 genetic variation with prospective IGF-I and IGFBP-3 blood levels and prostate cancer risk among Caucasians. (ox.ac.uk)
  • Therefore, increases in serum levels of this IGFBP result in a decrease of IGF1 activity thus inhibiting the cellular pathways. (proteopedia.org)
  • Comparison of IGFBP-3 with IGF-1 concentrations revealed that they did not exhibit the same developmental pattern in puberty. (nih.gov)
  • The association between diet and serum concentrations of IGF-I, IGFBP-1, IGFBP-2, and IGFBP-3 in the European Prospective Investigation into Cancer and Nutrition. (ox.ac.uk)
  • The objective of this study was to assess the association between dietary intake and serum concentrations of IGF-I, IGFBP-1, IGFBP-2, and IGFBP-3 in a cross-sectional analysis of 4,731 men and women taking part in the European Prospective Investigation into Cancer and Nutrition. (ox.ac.uk)
  • Serum concentrations of IGF-I, IGFBP-1, IGFBP-2 and IGFBP-3 were measured, and the associations between diet and IGF-I and IGFBPs were assessed using multiple linear regression adjusting for sex, age, body mass index, smoking status, and alcohol and energy intake. (ox.ac.uk)
  • The concentrations of IGF-1, IGFBP-1, and IGFBP-1/IGF-1 ratio were similar among HFpEF, HFmrEF, and HFrEF patients. (kent.ac.uk)
  • Conclusion: The concentrations of plasma IGFBP-1 and IGFBP-1/IGF-1 ratio can distinguish patients with and without HF. (kent.ac.uk)
  • Significant sex interactions, which were characterized by different genotype-phenotype associations between men and women, were found only for associations of IGFBP-3 concentrations with SNPs at the loci IGFBP3 and SORCS2. (ox.ac.uk)
  • Analyses of SNPs, gene expression, and protein levels suggested that interplay between IGFBP3 and genes within the NUBP2 locus (IGFALS and HAGH) may affect circulating IGF-I and IGFBP-3 concentrations. (ox.ac.uk)
  • In conclusion, this study identified several loci associated with circulating IGF-I and IGFBP-3 concentrations and provides clues to the potential role of the IGF axis in mediating effects of known (FOXO3) and novel (ASXL2) longevity-associated loci. (ox.ac.uk)
  • This IGFBP-6 antibody was prepared by immunising rabbits with a 15 amino acid peptide derived from the central domain of human IGFBP-6, linked to diphtheria toxoid. (gropep.com)
  • Although the sequence is derived from human IGFBP-6, the antibody does recognise IGFBP-6 from several species, although this should be experimentally determined. (gropep.com)
  • The IGFBP-2 in the samples binds quantitatively to the immobilized antibody. (eaglebio.com)
  • In the following step, the biotinylated antibody in turn binds IGFBP-2. (eaglebio.com)
  • ELISA: Rat IGFBP-5 ELISA Kit (Colorimetric) - These standard curves are provided for demonstration only. (assaysolution.com)
  • IGF-I levels increased to relatively higher levels than IGFBP-3, leading to an increasing molar ratio between IGF-I and IGFBP-3 in puberty, when growth velocity is high. (nih.gov)
  • We hypothesised that higher IGF-I, IGF-II, IGFBP-3 and IGF molar ratio would be associated with better cross-sectional and longitudinal lung function. (ox.ac.uk)
  • We examined cross-sectional (n=843) and prospective associations (n=717) between IGF-I, IGF-II, IGFBP-3 and IGF molar ratio with lung function in the Caerphilly Prospective Study (CaPS) from blood samples obtained around 1986, with spirometry (forced expiratory volume in one second (FEV1) and forced vital capacity (FVC)) performed in the same year and around 2003. (ox.ac.uk)
  • IGF-I, IGF-II and IGFBP-5 mRNA are present in adult human skeletal muscle, but no significant changes between meal groups were observed for IGF-I, IGF-II or IGFBP-5 mRNA levels, indicating that the expression of these genes are not altered acutely by nutrients and insulin. (diva-portal.org)
  • Increases in the amount of PAPP-A mRNA in granulosa cells during follicular development occurs in some but not all species, indicating that other proteases or protease inhibitors may be involved in IGFBP degradation. (bioone.org)
  • In some species (e.g., bovine), evidence indicates that decreases in IGFBP-4 and -5 levels in estrogen-dominant preovulatory follicles are likely due, in part, to increased protease activity, whereas lower levels of IGFBP-2 are not due to increased proteolysis. (bioone.org)
  • During the GH-therapy, e.g. in short stature and in GH-abuse (doping) the IGFBP-2 level decreases. (eaglebio.com)
  • These protease-induced decreases in IGFBP-4 and -5 likely cause increased levels of bioavailable (or free) IGFs that stimulate steroidogenesis and mitogenesis in developing dominant follicles, which ultimately prepare the follicle(s) and oocyte(s) for successful ovulation and fertilization. (bioone.org)
  • IGFBP-5-induced migration was blocked by the MEK1/2 inhibitor U0126, suggesting that IGFBP-5-induced migration occurs via MAPK activation. (elsevierpure.com)
  • Up to 5 freeze-thaw cycles did not change the measured IGFBP-2 concentration significantly. (eaglebio.com)
  • Moreover, the CD4/CD8 ratio of migrating cells was significantly higher in vitro and in vivo in response to IGFBP-5. (elsevierpure.com)
  • IGFBP-2 influences tumor cells as it induces catalase activity in adrenocortical cells (19). (eaglebio.com)
  • Collectively, our results suggest that IGFBP-5 induces cell migration via MAPK-dependent and IGF-I-independent mechanisms. (elsevierpure.com)
  • Yasuoka, H, Yamaguchi, Y & Feghali-Bostwick, CA 2009, ' The pro-fibrotic factor IGFBP-5 induces lung fibroblast and mononuclear cell migration ', American journal of respiratory cell and molecular biology , vol. 41, no. 2, pp. 179-188. (elsevierpure.com)
  • This Rodent IGFBP-2 ELISA Assay is suited for measuring IGFBP-2 in mouse and rat serum for scientific purposes. (eaglebio.com)
  • The serum concentration of IGFBP-2 increases in fasting, after major surgery and after trauma, but the increase of the concentration is most intensive in malignant diseases. (eaglebio.com)
  • The correlation of the IGFBP-2 level to the degree of progression is a striking feature in various tumor types as is the normalization of the IGFBP-serum levels after remission (5-10). (eaglebio.com)
  • Overexpression of the IGFBP-2 gene in mice results in a weight reduction of 30% in spleen and moderately reduced weight in other organs (18). (eaglebio.com)
  • IGFBP-3 levels are regulated by GH and have been suggested to provide additional information on GH secretory capacity compared to IGF-I. However, the diagnostic value of IGFBP-3 is still controversial, perhaps because the quality of the available normative data for IGFBP-3 varies. (nih.gov)
  • Therefore, we measured IGFBP-3, IGF-I, IGF-II, IGFBP-1, and IGFBP-2 levels by RIA in 907 healthy children to establish well characterized normative data on IGFBP-3 according to age, sex, and pubertal stage and to study the complex relationship between IGFs and their BPs in puberty. (nih.gov)
  • Age, sex, height, BMI, and pubertal maturation were all important factors in determining the circulating levels of IGFBP-3, whereas IGF-I levels were unaffected by BMI. (nih.gov)
  • Concomitantly, IGF-II and IGFBP-2 levels were unchanged throughout puberty, whereas IGFBP-1 levels declined with age in prepubertal children, with lowest values in puberty. (nih.gov)
  • Fetal cord blood IGF-I, IGFBP-1 and IGFBP-3 levels of appropriate-for-gestational-age (AGA) and intrauterine growth retardation (IUGR) babies are studied and followed up for 6-9 months, reevaluated for anthropometric measures and the effects of IGF-I, IGFBP-1 and IGFBP-3 on fetal growth and early catch-up growth is investigated. (deu.edu.tr)
  • IGF-I and IGFBP-3 levels were found to be high in AGAs with respect to IUGR babies and IGFBP-1 is found to be high in IUGR with respect to AGAs. (deu.edu.tr)
  • A good correlation was found between birth weight, postnatal weight and postnatal height and IGF-I and IGFBP-3 levels (p (deu.edu.tr)
  • In HF, IGFBP-1 and IGFBP-1/IGF-1 ratio positively correlated with NT-proBNP levels. (kent.ac.uk)
  • IGFBP-3 blood levels were 6.3% higher for each minor allele. (ox.ac.uk)
  • Genomewide meta-analysis identifies loci associated with IGF-I and IGFBP-3 levels with impact on age-related traits. (ox.ac.uk)
  • We recently observed mononuclear cell infiltration in lung tissues of mice expressing IGFBP-5. (elsevierpure.com)
  • Especially in tumor biology, the mouse and rat systems enable investigation of the systemic relevance of IGFBP-2. (eaglebio.com)
  • It is functionally similar to human IGFBP-5 and inhibits the effects of IGF peptides. (eaglebio.com)
  • Interestingly, IGFBP-5 also induced migration of primary human lung fibroblasts. (elsevierpure.com)
  • ALS increases the half-life of IGF/IGFBP complexes in circulation. (rndsystems.com)
  • Migration assays demonstrated that IGFBP-5 induced migration of peripheral blood mononuclear cells (PBMCs) in a dose-dependent manner. (elsevierpure.com)
  • IGFBP-5 resulted in preferential migration of activated CD4 + T cells and monocytes. (elsevierpure.com)
  • MiR-137 inhibited cell proliferation and migration of vascular smooth muscle cells via targeting IGFBP-5 and modulating the mTOR/STAT3 signaling. (genscript.com)
  • With the exception of IGFBP-3 and FEV1/FVC in the simple model (0.009, 95% CI 0.001-0.018, P=0.04) all prospective associations between IGF and spirometric measures were consistent with chance. (ox.ac.uk)
  • Insulin-like growth factors (IGFs) regulate the proliferation, differentation, apoptosis, cell adhesion and metabolism in various tissues and cell types IGFBP-2 is an unglycosylated polypetide of 31.3 kDa, which forms binary IGF-complexes and shows no circadian rhythm in the circulation. (eaglebio.com)
  • Associations of IGF-I, IGFBP-1 and IGFBP. (deu.edu.tr)
  • Role of IGF-I, IGF-II and IGFBP-3 in lung function of males: the Caerphilly Prospective Study. (ox.ac.uk)
  • The peptide sequence is unique to IGFBP-6. (gropep.com)
  • The intensity of the blue color depends on the IGFBP-2 content of the sample. (eaglebio.com)
  • The Mouse/Rat IGFBP-2 ELISA Assay Kit is for Research Use Only. (eaglebio.com)
  • The Mouse/Rat IGFBP-2 ELISA, is a so-called sandwich-assay. (eaglebio.com)
  • The mouse or rat model is a well-suited system for investigation of the relevance of IGFBP-2 in physiological and pathological processes. (eaglebio.com)
  • Increased IGFBP-4 and -5 protease along with lower amounts of IGFBP-4 binding activity and greater amounts of free IGF-I are some of the earliest developmental changes documented in bovine growing antral follicles. (bioone.org)