Identify mirnas regulated novel candidate mirnas micro rna that regulates. Medical search. Frequent questions

MicroRNAs constitute a pervasive post-transcriptional filter on protein or mRNA expression levels that are likely to control developmental timing, cellular differentiation, stress responses, metabolism and proliferation. (mdpi.com)
m6A mRNA methylation regulates human β-cell biology in physiological states and in type 2 diabetes. (dasmaninstitute.org)
The mature miRNA is incorporated into a RNA-induced silencing complex (RISC), which recognizes target mRNAs through imperfect base pairing with the miRNA and most commonly results in translational inhibition or destabilization of the target mRNA. (cancerindex.org)
Recently, miRNA-messenger RNA (mRNA) regulatory relationships have been confirmed during biological processes, including osteogenic differentiation. (frontiersin.org)
This study aimed to find out more candidate miRNA-mRNA pairs involved in the osteogenic differentiation of MC3T3-E1 cells. (frontiersin.org)
Multiple miRNA-mRNA prediction databases were searched by differentially expressed genes (DEGs) to obtain pairs of a miRNA-DEG regulatory network. (frontiersin.org)
The miRNA-mRNA interactions were verified by dual-luciferase reporter gene assays and experiments using mimics miRNA or their inhibitors. (frontiersin.org)
There were 7 overlapped miRNA-mRNA pairs identified during osteogenic differentiation of MC3T3-E1 cells, including mmu-miR-204-5p-Arhgap11a, mmu-miR-211-5p-Arhgap11a, mmu-miR-24-3p-H2afx, mmu-miR-3470b-Chek2, mmu-miR-3470b-Dlgap5, mmu-miR-466b-3p-Chek1, and mmu-miR-466c-3p-Chek1. (frontiersin.org)
MicroRNAs (miRNAs) are small noncoding RNAs regulating gene expression by mRNA targeting. (biomedcentral.com)
Incorporation of miRNAs in RNA-induced silencing complexes (RISCs) then leads to decay or to translational repression of complementary mRNA targets. (biomedcentral.com)
Integrated mRNA and miRNA profiling data provided comprehensive gene expression information on the wild-type and multi-tepal mutant at the transcriptional level that could facilitate our understanding of the molecular mechanisms of floral patterning of C. goeringii . (biomedcentral.com)
In particular, we have identified that promoter CpG island hypermethylation of the genes TUSC3 (tumor suppressor candidate 3), POMT1 (protein O-mannosyltransferase 1), ATRNL1 (attractin-like 1) and SAMD4A (sterile alpha motif domain containing 4A) is linked to the transcriptional downregulation of both linear mRNA and the hosted circRNA. (oncotarget.com)
Although some circRNAs regulate the linear transcript, we did not observe changes in TUSC3 mRNA levels upon TUSC3 circ104557 overexpression. (oncotarget.com)
miRNAs predominantly interact with mRNA targets through imperfect binding to motifs in target mRNA 3′-untranslated regions (3′UTRs) ( Bartel, 2009 ). (elifesciences.org)
The nature of imperfect binding specificity means that a single miRNA can regulate a large number of mRNA targets involved in complex cellular processes, thereby tightly controlling genetic networks during development and in response to stress ( Pocock, 2011 ). (elifesciences.org)
Pansensitive and panresistant genes to 21 NCCN-recommended drugs with concordant mRNA and protein expression were identified. (cdc.gov)

Furthermore, GSEA analysis revealed a significant up-regulation of the cell cycle, DNA replication initiation, ATP-dependent RNA helicase activity, and down-regulation of the TNF signaling pathway. (bvsalud.org)

MicroRNAs (miRNAs) are well-recognized for their abilities to regulate gene expression post-transcriptionally in plants and animals. (frontiersin.org)
MicroRNAs regulate target gene expression post-transcriptionally in a myriad of cell types and play critical roles in diverse physiological and pathological processes, including cardiomyocyte development, differentiation, and regeneration. (biomedcentral.com)
MicroRNAs (miRNAs) are small noncoding RNAs (~22 nucleotides in length) that regulate gene expression post-transcriptionally by imperfect binding to the 3′ untranslated region of target mRNAs in a wide variety of cell types. (biomedcentral.com)

Numbers shown below represent number of publications held in OncomiRDB database for Oncogenic and Tumor-Suppressive MicroRNAs. (cancerindex.org)
This may include oncomir (oncogenic miRNA) inhibition, or tumor suppressor-miRNA replacement therapies [ 6 , 9 ]. (hindawi.com)
Based on the consistent expression patterns associated with patient survival outcomes and in tumors vs. normal lung tissues, 10 miRNAs were considered to be putatively tumor suppressive and 4 miRNAs were deemed as oncogenic in lung cancer. (cdc.gov)

An aberrant miRNA expression could contribute to cancer development and progression [ 6 , 7 ] and could affect their target genes that are involved in many biological processes, such as cell differentiation, proliferation, apoptosis, metabolism, and development [ 8 ]. (hindawi.com)
Gain and loss of function studies have indicated that miRNAs play a critical role in the regulation of all key biological functions such as development, cell proliferation, cell differentiation, and apoptosis [ 3 , 4 ]. (hindawi.com)
TRIM13 inhibited cell proliferation and induced cell apoptosis by regulating NF-κB pathway in non-small-cell lung carcinoma cells. (cancerindex.org)

This Special Issue of "Genes" seeks reviews and original papers covering a wide range of topics related to microRNA biology, such as regulation of expression in various disorders (cancer, metabolism, autoimmunity to mention but a few), genetics of microRNAs and their target sites, functional analysis of microRNA function and studies of interactions between microRNAs and target genes. (mdpi.com)
microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. (cancerindex.org)
With respect to the reported regulation of this miRNA in Scrapie-infected mice, we propose that upregulation of hsa-miR-342-3p may be a general phenomenon in late stage prion disease and might be used as a novel marker for animal and human TSEs. (biomedcentral.com)
Thus we hypothesized that miRNA regulation may also play a role in human prion diseases. (biomedcentral.com)
The discovery of miRNAs has revolutionized our comprehension of the regulation of gene expression. (hindawi.com)
Interestingly, we found circRNA-mediated regulation of target miRNAs and an in vivo growth inhibitory effect upon TUSC3 circ104557 transduction. (oncotarget.com)
The day-to-day fluctuations that most investigators focus upon are not found in this latest group, suggesting that short-term and long-term miR regulation are differentially regulated. (ca.gov)
Given the notion that a single miRNA may have multiple cellular targets and given the existence of vast numbers of miRNAs (~1,500 in humans), we can expect to witness the discovery of novel miRNA-dependent regulation in the modulation of versatile biological functions [ 4 ]. (biomedcentral.com)

MicroRNAs (miRNAs) is a large family of endogenous, small noncoding RNAs with 20-25 nucleotides that have emerged as key posttranscriptional regulators of gene expression in mammals, bacteria, and plants [ 1 - 4 ]. (hindawi.com)

There were 1024 differentially expressed genes (DEGs) identified by transcriptome analysis between the LBW and NBW placentas, of which 218 and 806 genes were up- and down-regulated in the LBW placentas, respectively. (bvsalud.org)
Among them, 18,489 were identified as differentially expressed genes between the two libraries according to comparative transcript profiling. (biomedcentral.com)

Finally, we identified 76 therapeutic agents, 152 miRNAs targets, and 91 TFs regulatory networks. (nature.com)
and the miR319/TCP4-miR396/GRF regulatory cascade probably regulating cell proliferation of the multi-tepal development. (biomedcentral.com)
Genes controlling floral organ identity have been identified through the genetic analysis of homeotic mutants, leading to the ABCDE model, in which five classes of regulatory genes (A, B, C, D and E) work in a combinatorial manner to confer the organ identities of the four whorls [ 10 ]. (biomedcentral.com)
In addition, due to their rapid and reversible regulatory capacity, miRNAs are prime candidate facilitators of responses to proteotoxic stress. (elifesciences.org)
miRNAs are noncoding, regulatory RNAs expressed dynamically during differentiation of hESC. (ca.gov)

Investigation of hub genes for the development of potential therapeutic targets and candidate biomarkers is warranted. (nature.com)
The findings may help to provide promising candidate biomarkers and therapeutic targets for AD. (nature.com)
Recent studies reported microRNAs as promising biomarkers for early cancer detection, accurate prognosis, and molecular targets for future treatment. (hindawi.com)

This class of noncoding RNAs is small, single stranded, and 19-25 nucleotide long that act as negative regulators involved in posttranscriptional silencing of the gene expression [ 5 ]. (hindawi.com)

miRNAs are transcribed by RNA polymerase II as part of capped and polyadenylated primary transcripts (pri-miRNAs) that can be either protein-coding or non-coding. (cancerindex.org)
MiRNAs are derived from primary transcripts (pri-miRNA) that are sequentially processed into their mature form by the RNase III type nucleases DROSHA and DICER [ 5 ]. (biomedcentral.com)

Specifically, some members of the family (ANGPTL3, 4 and 8) were shown to regulate LPL enzymatic activity. (dasmaninstitute.org)

Herein, we wondered whether circular RNAs (circRNAs), a type of RNA transcripts lacking 5′-3′ ends and forming closed loops that are gaining relevance in cancer biology, are also a target of epigenetic inactivation in tumors. (oncotarget.com)
Approximately just 2% of the genome is transcribed into protein-coding RNAs [ 1 ], so the majority of transcripts are noncoding RNAs (ncRNAs), that can be categorized according to their structural properties and length [ 2 ]. (oncotarget.com)

Our study identified 9 key genes associated with oxidative stress and immune reaction in AD pathogenesis. (nature.com)

The immune-related DEOSGs and hub genes were identified by weighted gene co-expression network analysis (WGCNA) and protein-protein interaction (PPI) analysis, respectively. (nature.com)
We also studied the microRNAs that affect NEK2 expression. (oncotarget.com)
Studies are also showing differential expression levels of miRNAs and ANGPTLs in obese versus non-obese individuals. (dasmaninstitute.org)
However, understanding how glutamine affects gene expression in the intestinal epithelium is limited, and identifying the essential genes and signals involved in regulating intestinal epithelial cell growth is particularly challenging. (bvsalud.org)
It regulates Sp7 protein expression and induces expression of major bone matrix protein genes, such as Col1a1, Spp1, Ibsp, Bglap2, and Fn1 ( 7 , 8 ). (frontiersin.org)
The aim of our study was to analyze the differential expression of miRNAs in the brains of BSE-infected cynomolgus macaques as a model for Creutzfeldt-Jakob disease (CJD). (biomedcentral.com)
To investigate whether prion-induced neurodegeneration is linked to deregulation of miRNA in the brain of affected individuals, we analyzed differential miRNA expression in brains of BSE-infected non-human primates ( Macaca fascicularis ) as a model for Creutzfeldt-Jakob disease in humans. (biomedcentral.com)
For the identification of deregulated miRNAs we applied miRNA microarrays which have been widely used to analyze miRNA expression patterns. (biomedcentral.com)
miRNAs are transcribed as ~70 nucleotide stem-loop precursors and subsequently processed by the cytoplasmic RNase-III type enzyme Dicer to generate ~22 nucleotide mature products which can target and modulate gene expression by inhibiting translation and/or inducing degradation of target mRNAs [ 4 , 6 , 7 ]. (hindawi.com)
Mapping developmental expression of miRNAs during transition from pluripotency to pancreatic progenitors will help clarify the mechanisms underlying lineage specification and ultimately enhance differentiation protocols. (ca.gov)
Aim 1 - Generate miR expression profiles using deep sequencing for defined stages of development from pluripotent to endocrine cells and select candidate miRs for manipulations involving silencing and overexpression. (ca.gov)
A) Paired microRNA expression and development of a reporter system for lineage fate. (ca.gov)
B) Deep sequence purified hESC populations from selected time points during hESC differentiation and develop algorithms to analyze change in miRNA expression in complex systems. (ca.gov)
By employing microRNA expression profiling and functional knockdown studies on human embryonic stem cell-derived cardiomyocytes, the authors identified miR-363 as an upstream negative regulator of left ventricular specification transcription factor HAND1 . (biomedcentral.com)
To this end, the authors performed miRNA expression profiling in undifferentiated hESCs and CMs at day 8 and day 14 after differentiation. (biomedcentral.com)
DEFB1 expression after transfection with two micro RNAs (miRNAs), hsa-miR-186-5p and hsa-miR-340-5p, provided evidence that DEFB1 expression could be modulated by these miRNAs and hsa-miR-186-5p had a higher binding efficiency with DEFB1. (cdc.gov)

Identifying molecular regulators and critical mediators of cardiac cell type development, proliferation, and differentiation is of great clinical importance, and unraveling such molecular horizons could lead to the development of therapeutic strategies for successful regeneration of the human adult heart. (biomedcentral.com)
Experimentally confirmed target genes were identified for the 73 diagnostic miRNAs, from which proliferation genes were selected from CRISPR-Cas9/RNA interference (RNAi) screening assays. (cdc.gov)

We have applied miRNA-microarrays to identify deregulated miRNA candidates in brains of BSE-infected macaques. (biomedcentral.com)

Since Sirt2 plays a critical role in multiple important cellular functions, our data imply that acupuncture may act through epigenetic changes and subsequent action on their targets, such as miRNA-339/Sirt2/NF- B/FOXO1 axis. (hindawi.com)
However, the targets of acupuncture-regulated miRNAs are not fully identified and validated and it is unknown whether this is involved in acupuncture's effects. (hindawi.com)
We are working under the postulate that miRNAs are logical targets for in vitro experimentation because of their role in mediating pancreatic cell development. (ca.gov)
Aim 2 - Identify miRs targets through deep sequencing of RNA induced silencing complexes (RISC) in defined cell populations and assessment of their roles in differentiation in vitro and after experimental transplantation. (ca.gov)
miRNAs could thus be the best targets for understanding cardiac specialization during differentiation of human embryonic stem cells (hESCs). (biomedcentral.com)

MicroRNA-34a gene (MIR-34A) that is located on chromosome 1p36 belongs to one of evolutionary-conserved miRNA families (MIR-34 family) that consists of three members: MIR-34A, MIR-34B, and MIR-34C [ 10 ]. (hindawi.com)

However, the role of microRNA in mammalian tissue development and differentiation is still quite uncharacterized. (mdpi.com)
The long term goal of our research is to understand the biochemical processes that regulate differentiation of human embryonic stem cells (hESCs) into pancreatic progenitor cells, and ultimately, glucose-responsive, insulin producing (beta) β cells. (ca.gov)
Of the several genetic factors that contribute to stem cells differentiation, miRNAs (microRNAs) are emerging as important determinants. (ca.gov)

The expressions of candidate miRNAs and mRNAs were determined by real-time quantitative PCR(RT-qPCR) in MC3T3-E1 cells. (frontiersin.org)
The mature miRNA is incorporated into a RNA-induced silencing complex (RISC), which recognizes target mRNAs through imperfect base pairing with the miRNA. (hindawi.com)
miRNAs act as adaptors that employ a silencing complex to target mRNAs by selective base pairing, primarily in the 3′-untranslated region (3′-UTR). (hindawi.com)

These findings suggest miR-486-5p negatively regulates NEK2, which is a critical prognostic indicator of HCC patient survival after liver transplantation. (oncotarget.com)

A total of 1160 DEGs (259 up-regulated and 901 down-regulated) were screened in GSE48350. (nature.com)
We identified 715 upregulated DEGs and 603 downregulated DEGs between MC3T3-E1 cells with and without osteoblast induction by analyzing the raw data of the GSE46400 dataset. (frontiersin.org)

In addition, Ingenuity Pathway Analysis (IPA) integrated with PCR array data showed that the JAK1/STAT3 pathway was significantly altered in cells overexpressing DEFB1, suggesting this to be one of the pathways by which defensin regulates IAV replication in HBEpCs. (cdc.gov)

The microRNA processor DROSHA is a candidate gene for a severe progressive neurological disorder. (mdanderson.org)

Recently, the potential therapeutic use of miRNAs has been evaluated due to their dynamic and reversible properties. (hindawi.com)
Because miRNAs are easy to synthesize and deliver, miR-7 could be a potential therapeutic means to macrocephaly caused by Gli3-deficiency. (frontiersin.org)

The primary transcript is cleaved by the Drosha ribonuclease III enzyme to produce an approximately 70-nt stem-loop precursor miRNA (pre-miRNA), which is further cleaved by the cytoplasmic Dicer ribonuclease to generate the mature miRNA and antisense miRNA star (miRNA*) products. (cancerindex.org)

Minogue AL, Arur S*. In Situ Hybridization for Detecting Mature MicroRNAs In Vivo at Single-Cell Resolution. (mdanderson.org)

Recent studies have revealed that a group of miRNAs are involved in the neural mechanism of acupuncture treatment in hypertensive rats [ 30 ]. (hindawi.com)

Based on miRBase version 21 released in June 2014 ( http://www.mirbase.org/ ), there are 1,881 miRNA precursors and 2,588 mature miRNAs in humans. (hindawi.com)

In conclusion, the reduction in IAV copy number in DEFB1 overexpressing cells suggests that beta-defensin-1 plays a key role in regulating IAV survival through STAT3 and is a potential target for antiviral drug development. (cdc.gov)

We have found that master regulators of miRs exist and/or are being regulated by another regulator but that regulator is biasing them over a large time scale (weeks). (ca.gov)

Since the first microRNA was identified in 1993 by Victor Ambros and colleagues (the lin-4 heterochronic gene in C. Elegans ), more than 24,000 microRNAs have now been identified. (mdpi.com)
Here, we report that the microRNA miR-1 regulates the autophagy pathway through conserved targeting of the orthologous T re-2/ B ub2/ C DC16 (TBC) Rab GTPase-activating proteins TBC-7 and TBC1D15 in Caenorhabditis elegans and mammalian cells, respectively. (elifesciences.org)
Furuta, T, Arur S . sart -3 functions to regulate germline sex determination in C. elegans . (mdanderson.org)

RNA sequencing indicated diverse transcriptome changes between the control and glutamine supplementation groups, identifying 925 up-regulated and 1152 down-regulated genes. (bvsalud.org)

The role of epigenetic factors such as microRNAs in regulating many biological processes associated with obesity and diabetes including lipid metabolism has been recently highlighted. (dasmaninstitute.org)

Simple Summary: This study identified a set of 73 microRNAs (miRNAs) that can accurately detect lung cancer tumors from normal lung tissues. (cdc.gov)
This study identified a set of 73 microRNAs (miRNAs) that classified lung cancer tumors from normal lung tissues with an overall accuracy of 96.3% in the training patient cohort (n = 109) and 91.7% in unsupervised classification and 92.3% in supervised classification in the validation set (n = 375). (cdc.gov)

In human SH-SY5Y cells, the luciferase assay implied that Sirt2 was likely a target of miRNA-339. (hindawi.com)
Small RNA sequencing revealed 132 conserved miRNA families expressed in flowers of C. goeringii , and 11 miRNAs corresponding to 455 putative target genes were considered to be responsible for multi-tepal development. (biomedcentral.com)

The RefSeq represents the predicted microRNA stem-loop. (cancerindex.org)

Dynamic transcriptomic m(6)A decoration: writers, erasers, readers and functions in RNA metabolism. (dasmaninstitute.org)

Recently, we have found that a number of microRNAs (miRNAs) and proteins are involved in the response to acupuncture therapy in hypertensive rats. (hindawi.com)
Our bioinformatics study suggests an association between these miRNAs and proteins, which include miR-339 and sirtuin 2 (Sirt2). (hindawi.com)

Our findings demonstrate that miR-339 regulates Sirt2 in human and rat neurons. (hindawi.com)

Noncoding RNAs (ncRNAs), such as microRNAs and long noncoding RNAs (lncRNAs), participate in cellular transformation. (oncotarget.com)
We believe that the results from this project have the potential to create a paradigm shift in understanding the cellular ontogeny of the pancreas and help identify which cell types can be used for transplantation therapy in T1D. (ca.gov)

From the list of genes that were targeted by the 73 diagnostic miRNAs, DGKE and WDR47 had significant associations with responses to both systemic therapies and radiotherapy in lung cancer. (cdc.gov)

Specifically, the objectives of this CIRM grant are to elucidate the role miRNAs play in the development of hESC into cells of endocrine lineage and to provide crucial details on the molecular architecture of endocrine precursor populations, lineage specification, and β-cell maturation. (ca.gov)
p53 has a crucial but complex role in regulating ferroptosis. (nature.com)
Further exploring the role of miRNAs in cardiac cells during development and disease may therefore hold great promise for cardiac therapy applications. (biomedcentral.com)

Finally, we constructed the drug, transcription factors (TFs), and microRNA network of the diagnostic genes. (nature.com)

The central hypothesis driving the research is that miRNAs are essential regulators of endocrine cell development. (ca.gov)
Other labs have shown that miR-375 is also expressed in pancreatic during development, and specifically in beta cells in mature islets, where it regulates insulin secretion. (ca.gov)
Recently, miRNAs have been recognized as important players in cardiac development, pathology, and regeneration. (biomedcentral.com)

miRNAs are predicted to control the activity of more than 30% of human genes [ 5 ]. (hindawi.com)
miRNAs are also associated with many human pathologies such as cancer, heart diseases, diabetes, inflammatory diseases, autoimmune diseases, and Alzheimer's disease [ 1 , 8 - 17 ]. (hindawi.com)

Conversely, miR-122 is the most highly expressed miRNA in liver, which arises in the region of endoderm that is closest to the pancreatic buds. (ca.gov)

Thereafter, we identified significant difference of infiltrated immune cells (effector memory CD8 T cell, activated B cell, memory B cell, natural killer cell, CD56 bright natural killer cell, natural killer T cell, plasmacytoid dendritic cell, and neutrophil) between AD and control samples. (nature.com)

Anatomy2

Organisms2

Diseases1

Chemicals and Drugs16

Analytical, Diagnostic and Therapeutic Techniques and Equipment9

Phenomena and Processes16

Disciplines and Occupations1

Information Science2

Health Care2

MRNA16

Signaling pathway1

Gene expression post-transcriptionally3

Oncogenic3

Apoptosis3

Regulation8

Nucleotides1

Differentially expressed genes2

Regulatory5

Biomarkers3

Single stranded1

Primary transcripts2

Shown to regulate1

Transcripts2

Oxidative stress1

Expression16

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Targets5

Chromosome1

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MRNAs3

Negatively1

DEGs2

Pathways1

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Potential therapeutic2

Dicer1

Vivo1

Neural1

Humans1

Survival1

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Elegans3

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