• Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used to identify the targets recognized by circulating AAbs seen on 2D-GE, followed by ELISAs with recombinant proteins to confirm LC-MS/MS results, and quantify autoreactivities. (duke.edu)
  • Identified targets may be mechanistically linked to AMD pathogenesis because the identified proteins are implicated in autophagy, immunomodulation, and protection from oxidative stress and apoptosis. (duke.edu)
  • Based on immunological and mass spectrometric analysis, SALSA was estimated to constitute up to 4-10% of the total protein amount in meconium, making it one of the most abundant proteins. (researchgate.net)
  • The matching process allows the researcher to identify the proteins. (studykraken.com)
  • Residual host proteins may identify sources of infection for ticks. (cdc.gov)
  • Proteins of pooled ticks were separated by 1-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and gel slices were digested in situ with trypsin before using LC-MS/MS. Upwards of ≈7,000 spectra were submitted to a protein identification algorithm for each pooled sample. (cdc.gov)
  • The SEQUEST search algorithm ( http://fields.scripps.edu/sequest ) with data-filtering criteria was used to identify sequence matches of output against databases of rabbit, sheep, deer, goat, mouse ( Mus musculus ), and tick proteins. (cdc.gov)
  • MudPIT analysis identified 205 proteins in cervical-vaginal fluid (CVF), 28 of which exhibited significant differences in pairwise and progressive comparisons. (elsevierpure.com)
  • 2D-DIGE identified 17 proteins that were significantly differentially present in PTL and SPTB. (elsevierpure.com)
  • We developed an efficient protein extraction method and identified some of the seed proteins. (usda.gov)
  • We investigated whether isopropanol extraction could facilitate resolution of the less abundant proteins from soybean seeds in one dimensional polyacrylamide gel electrophoresis (1D-PAGE) and two-dimensional polyacrylamide gel electrophoresis (2D-PAGE). (usda.gov)
  • We have identified a total of 107 protein spots using Matrix-assisted laser desorption/ionization time of flight mass spectrophotometry (MALDI-TOF-MS) and liquid chromatography mass spectrometry (LC-MS/MS). Our results suggest that extraction of soybean seed powder with 40% isopropanol enriches for lower abundance proteins and is a suitable method for 2D-PAGE separation and identification. (usda.gov)
  • From this, we identified 47 proteins that differed in abundance between cognitively normal: Clinical Dementia Rating [CDR] 0) and mildly demented: CDR 1) subjects. (wustl.edu)
  • Canonical pathway analysis revealed the identified host proteins are mainly involved in the coagulation cascade, complement pathway or acute phase response signaling pathway. (gabriel-network.org)
  • ELISAs were used to validate differential expression of a selection of identified host proteins in individual plasma samples of patients with dengue fever compared to patients with severe dengue. (gabriel-network.org)
  • A novel technique of virion-enrichment from plasma has allowed to identify two host proteins that have prognostic value for classifying patients with acute dengue who are more likely to develop a severe dengue. (gabriel-network.org)
  • In the current short review we focused on the proteins of the spermatozoa identified by MS proteomics technology. (jri.ir)
  • Techniques used in human sperm proteome mapping: There are several initial reports using MS proteomics technology to identify a limited number of proteins from the human sperm using twodimensional gel electrophoresis (2-DE) coupled to MALDI-TOF-MS analysis (16 - 23). (jri.ir)
  • An extensive human sperm proteome analysis using 1D-SDS-PAGE combined with electrospray liquid chromatography tandem mass spectrometry (GeLC-MS/ MS) approach identified 1,760 proteins (24). (jri.ir)
  • Among the identified candidate proteins, a downregulated 14-3-3ζ protein was chosen and confirmed by Western immunoblotting. (tmu.edu.tw)
  • Unknown interaction proteins can be identified. (mitochondriasci.com)
  • Objectives: To identify differentially expressed serum proteins amongst 3 primary stages of HCV infection and wholesome folks, and their comparisons with sera from patients using the very same stage of HBV infection. (glucagon-receptor.com)
  • P 0.05), of which 42 spots that corresponded to 15 proteins had been identified by liquid chromatography-tandem mass spectrometry. (glucagon-receptor.com)
  • There is a possibility to lose the analyte while extraction, electrospray is not always available, the sensitivity may depend on a number of factors, the problems may appear with chromatography if the stages of the procedure are confused, and the matrix is added to the mobile phase before the column (Faust 2005). (studykraken.com)
  • Mass spectrometry-based proteomics of individual ticks demonstrated persistence of mammalian host blood components, including α- and β-globin chains, histones, and mitochondrial enzymes, in Ixodes scapularis and Amblyomma americanum ticks for months after molting. (cdc.gov)
  • We used mass spectrometry (MS)-based proteomics, as described by Breci et al. (cdc.gov)
  • Utilizing an alternative, targeted proteomics approach to identify novel biomarkers, 333 CSF samples were evaluated for levels of 190 analytes using a multiplexed Luminex platform. (wustl.edu)
  • New advances in mass spectrometry-based proteomics technology are having amajor impact on our understanding of how human spermatozoa acquire their capacity for fertilization. (jri.ir)
  • Recently, use of mass spectrumetrybased (MS) proteomics technology has further contributed to the identification of the proteome that make up spermatozoa (6 - 12). (jri.ir)
  • Two common solid phase extractants, Tenax GC and octadecylsilane bonded silica gel (C18 Silica), were compared by Caton et al. (cdc.gov)
  • The extract was then fractionated in a silica gel column using previously determined solvent combinations as eluent. (ufh.ac.za)
  • This is based on thin-layer chromatography (TLC) performed on silica gel [ 13 ] . (encyclopedia.pub)
  • Active compounds are identified on the basis of their Rf values, while nonidentifiable active volatile compounds can be cut out from the silica gel and analyzed using the headspace solid-phase microextraction method coupled to gas chromatography-mass spectrometry (HS-SPME/GC-MS) [ 14 ] . (encyclopedia.pub)
  • With this, the purity or percentage composition of antimicrobial active compounds from silica gel can be determined. (encyclopedia.pub)
  • We subsequently compared IsDAED approach and traditional approach, and demonstrated identifying SI peptides and excluding NSI peptides supports efficient diagnostic kit development, specifically using a protein-peptide hybrid microarray (PPHM). (bvsalud.org)
  • To identify the potential ACE-inhibitory peptide, the most active subfraction was analysed using LC-MS/MS and peptides were identified using PEAKS Studio software. (bioinfor.com)
  • Active fractions obtained from column chromatography separations were further fractionated and the compounds identified by gas chromatography/mass spectrometry (GC/MS) analysis. (ufh.ac.za)
  • To identify active compounds responsible for the antilisterial activity of an EO, bioautography is a proper method. (encyclopedia.pub)
  • This technique is not only suitable for mass spectrometry analysis of protein mixtures after SDS-PAGE separation and in gel enzymolysis (in-gel digestion), but also suitable for mass spectrometry analysis of direct enzymolysis (in-solution digestion) in protein mixtures. (mitochondriasci.com)
  • An alternative approach to measure diisopropyl methylphosphonate in meat or milk is based upon thermal desorption into an ion trap mass spectrometer (TDITMS) (Buchanan et al. (cdc.gov)
  • Rather, the intention is to identify well-established methods that are used as the standard methods of analysis. (cdc.gov)
  • This review summarizes the combination of mass spectrometry and surface plasmon resonance (SPR) biosensor analysis for identification and affinity determination of protein interactions with antibodies and DNA-aptamers. (bvsalud.org)
  • The gels were stained with Sypro, and a comparison between CFS subject and the healthy one was performed by the software Progenesis Same Spot including the Analysis of variance (ANOVA test). (biomedcentral.com)
  • Gas chromatography (GC), combined with detection by mass spectrometry (MS), and proton nuclear magnetic resonance are then used to identify the fractions (Weiss et al. (cdc.gov)
  • These methods include separation by GC coupled with FID and flame photometric detection (FPD), determination by infrared and Raman spectroscopy, separation by ionization mass spectrometry, determination utilizing piezoelectric crystals, and determination by gas-sensitive microsensors. (cdc.gov)
  • hexane/diethylether (HDE), ethyl acetate/methanol/water (EMW) and chloroform/ethyl acetate/formic acid (CEF) in order to determine the most suitable combination for column chromatography (CC) and subsequent testing by indirect bioautography. (ufh.ac.za)
  • The relative molecular mass (M r ) of authentic exogenous Thap1 was previously established by in vitro translation of recombinant c-Myc-tagged human Thap1 protein from a human THAP1 cDNA. (biomedcentral.com)
  • 0 conversion) as well as the best CSF biomarkers identified to date, tau/A├ó42 and p-tau181/A├ó42. (wustl.edu)
  • CONCLUSIONS: Consistent with other evidence supporting the role of inflammation and the immune system in AMD pathogenesis, AAbs were identified in AMD sera, including early-stage disease. (duke.edu)
  • A proteolytic peptide mixture is then applied to the chip, and following removal of unbound material by washing, the epitope(s) peptide(s) are eluted and identified by MALDI-MS. The SPR-MS combination was applied to a wide range of affinity pairs. (bvsalud.org)
  • RRM1 , a ubiquitously expressed subunit of ribonucleoside-diphosphate reductase, and CCAR1 (cell cycle and apoptosis regulator 1) have been identified as direct targets for regulation by Thap1 in endothelial or Jurkat cells. (biomedcentral.com)
  • A clear genotype/phenotype relationship has not been established, nor is it definitively known that alterations in transcription of Thap1 downstream targets are responsible for DYT6, as Thap1 may have other, yet to be identified functions. (biomedcentral.com)
  • Our results have thus identified mucosal body compartments, where SALSA is particularly abundant, and suggest that SALSA exhibits varying functions in the different mucosal locations. (researchgate.net)
  • Use of PCR to identify DNA of vertebrate mitochondria in ticks has been reported ( 5 , 6 ), but results lacked full sensitivity ( 7 , 8 ). (cdc.gov)
  • Thus, in addition to the predicted 25 kDa apparent M r , we identified Thap1 species with greater apparent M r that we speculate may be a result of posttranslational modifications. (biomedcentral.com)
  • Exploration and exploitation of available -omics datasets, and the current trend to generate new ones will increase the opportunities to identify new target species, new bioactivities and new marine natural products from microalgae. (mdpi.com)
  • This study identified a para-chlorobenzyl analog, termed ML398 [2], with excellent potency, though it lacked the ability to be a PET tracer [2]. (vanderbilt.edu)
  • At the same time, mass spectrometry can fail to identify the components of a mixture if the analyte of interest is considered to be part of the mixture. (studykraken.com)
  • Methods used for analyzing diisopropyl methylphospbonate in air include real-time analyses utilizing interdigitated gate electrode-field effect transistors (IGEFET), coated and uncoated piezoelectric crystal detectors, gas chromatographs, and mass spectrometers. (cdc.gov)
  • But from time to time it can also be frustrating because of lack of critical mass in non-core but technically interesting areas. (lu.se)
  • The sample preparation stage usually identifies the outcome of the research. (studykraken.com)
  • This comparison will allow scientists to identify unintended effects that may occur when new transgenic soybean cultivars are developed and will help to improve consumer acceptance of GM soybeans. (usda.gov)
  • Most classification systems included primary (ie, in the sense of idiopathic) amyloidosis, in which no associated clinical condition was identified, and secondary amyloidosis, which is associated with chronic inflammatory conditions. (medscape.com)