The Primus instrument is a fully automated glycohemoglobin analyzer, which utilizes the principle of boronate affinity high performance liquid chromatography (HPLC)P 11dP. (cdc.gov)
Pulsed field gel elect1
In 3 households sampled, M. avium sharing rep -PCR and pulsed field gel electrophoresis fingerprints identified M. avium isolates clonally related to the patients' isolates. (cdc.gov)
Size-exclusion chro2
Each protein was purified by affinity and size-exclusion chromatography, using His-bind Ni2+-NTA resin (Invitrogen) and Superdex 200 resin (AKTA purifier), respectively. (mundial-brasil2014.net)
Carotenoid-protein complex (CPC) was isolated from chromoplast-enriched suspensions of cassava storage root (CSR) using size exclusion chromatography and characterized. (openbiochemistryjournal.com)
Liquid Chromatography3
In order to fill this technologic gap, we have used a functional proteomics approach using a liquid chromatography tandem mass spectrometric (LC-MS/MS) analysis coupled with a hydrazide biotin-streptavidin methodology in order to identify protein carbonylation in aged mice. (johnshopkins.edu)
DLS offers, again, free-- three free-- e-learning courses related to genetic testing, good laboratory practices for molecular genetic testing, good laboratory practice recommendations for biochemical, genetic testing, pre-analytic phase, and establishment and validation of new tests using liquid chromatography, and biochemical genetic laboratories. (cdc.gov)
Affinity1
These diol groups provide the basis for separation of glycated and non-glycated components by boronate affinity chromatography 11a,11b,11c. (cdc.gov)
Peptide1
Protein and peptide chromatography prior to MS analysis can partly address this issue. (hindawi.com)
Fractions2
Active fractions obtained from column chromatography separations were further fractionated and the compounds identified by gas chromatography/mass spectrometry (GC/MS) analysis. (ufh.ac.za)
Proteins identified within the fraction with monocyte promigratory activity and not in other inactive fractions included vimentin, PEDF, and TIMP-1. (hindawi.com)
Silica gel2
For low concentrations of methyl parathion, further cleanup procedures, such as column chromatography on silica gel or Florisil are required. (cdc.gov)
The extract was then fractionated in a silica gel column using previously determined solvent combinations as eluent. (ufh.ac.za)
Biochemical2
Over 300 proteins were identified in the releasate, with a wide range of annotated biophysical and biochemical properties, in particular platelet activation, adhesion, and wound healing. (hindawi.com)
Identifying and understanding the biochemical changes that sensitize the ageing brain to neurodegeneration will provide new opportunities for dementia prevention and treatment. (biomedcentral.com)
Proteins identified2
Plasma has a significant dynamic range, with more than 10 orders of magnitude separating albumin concentration and the rarest measurable proteins identified to date [ 10 ]. (hindawi.com)
Additionally, analytical methods are included that modify previously used methods to obtain lower detection limits and/or to improve accuracy and precision. (cdc.gov)
The analytical column contains aminophenylboronic acid bonded to a porous polymer support (gel). (cdc.gov)
Hexane1
hexane/diethylether (HDE), ethyl acetate/methanol/water (EMW) and chloroform/ethyl acetate/formic acid (CEF) in order to determine the most suitable combination for column chromatography (CC) and subsequent testing by indirect bioautography. (ufh.ac.za)
Degradation4
That's why the plant professional needs to understand the role of oil analysis in root cause analysis and in particular, how to use oil analysis to identify causes of accelerated oil degradation. (plantservices.com)
The test identifies "soft" contaminants (those directly associated with oil degradation) but isn't strongly influenced by larger, "hard" contaminants unrelated to oil degradation. (plantservices.com)
As a primary test that specifically identifies fluid degradation, QSASM is considered to be highly sensitive and reliable for detecting subtle changes in levels of insolubles. (plantservices.com)
It measures chemistry changes in the fluid base stock in addition to identifying degradation mechanisms. (plantservices.com)
Biological3
The primary method for detecting methyl parathion and metabolites in biological tissues is gas chromatography (GC) coupled with electron capture (ECD), flame photometric (FPD), or flame ionization detection (FID). (cdc.gov)
Proteomic approaches have proven powerful at identifying large numbers of proteins, but there are fewer reports of functional characterization of proteins in biological tissues. (hindawi.com)
Recent pharmacological studies have confirmed the known traditional uses of these species, elucidated previously unknown mechanisms of biological action, and found evidence of new biological activities, such as anticancer, cytotoxic, antiallergic, and neuroprotective activities. (researchgate.net)
Mass3
Historically, ROS targets have been identified by conventional immunological two-dimensional (2-D) gel electrophoresis and mass spectrometric analyses. (johnshopkins.edu)
In addition to high-abundance cytosolic proteins that have been previously identified by 2-D gel electrophoresis and mass spectrometric analyses, we are able to identify several low-abundance receptor proteins, mitochondrial proteins involved in glucose and energy metabolism, as well as a series of receptors and tyrosine phosphatases known to be associated with insulin and insulin-like growth factor metabolism and cell-signaling pathways. (johnshopkins.edu)
Mass spectrometry-based proteomic and lipidomic analysis of CA1 hippocampus samples from 74 neurologically normal human donors, aged 66-104, was used in combination with multiple regression models and gene set enrichment analysis to identify age-dependent changes in the proteome and lipidome. (biomedcentral.com)
Addition1
In addition, we were able to identify seven long tR inhibitors (100-1500 min) and to accurately determine their tR values. (mundial-brasil2014.net)
Approach2
A previous study from our laboratory using a MuDPIT (multidimensional protein identification technology) approach identified over 300 proteins secreted by platelets upon thrombin activation [ 1 ]. (hindawi.com)
The method was then used to measure tR as a function of temperature, an analysis not previously possible using the standard kinetic approach due to decreased NAD(P)H stability at elevated temperatures. (mundial-brasil2014.net)
Specifically1
Four P450s were recognized in immunoblot analyses of human liver microsome samples developed with an antibody previously demonstrated to specifically recognize rat 2B1/2. (nih.gov)
Root1
Lack of knowledge doesn't lead to a failure to identify a root cause correctly. (plantservices.com)
Methods1
Rather, the intention is to identify well-established methods that are used as the standard methods of analysis. (cdc.gov)
Order to identify1
In order to identify P450UK, it was purified and subjected to amino-terminal amino acid analysis. (nih.gov)
Western1
The presence of PEDF and involucrin, two proteins not previously reported in platelet releasate, was confirmed by western blotting. (hindawi.com)
Studies2
Previous proteomic studies of intact platelets have collectively identified hundreds of proteins using a variety of fractionation strategies including 2-dimensional electrophoresis (2DE), multidimensional chromatographic separations, membrane prefractionation techniques, and adsorption to combinatorial hexapeptide ligand libraries [ 1 - 6 ]. (hindawi.com)
Our initial studies suggest an ability to identify at least 100 carbonylated proteins in a single LC-MS/MS experiment. (johnshopkins.edu)
Results1
Results from qRT-PCR helped identify an isoform of HSP21 possessing four single point mutations in the intense yellow CSR that may be responsible for increased sequestration of b-carotene. (openbiochemistryjournal.com)
Samples1
In an effort to identify and characterize minor forms of human liver cytochrome P450, immunoblot analyses of microsome samples were developed with antibodies to various P450s that recognized multiple human P450s. (nih.gov)