Identification of specific antibodies against bacterial antibodies bacterial. Medical search. Frequent questions

This combination of effector cells requires a crosslinking antigen to evoke an antibody driven PMN inflammatory response via their Fc receptors. (bmj.com)
Changes in mucosal bacteria, and a switch from internal to surface antigen/antibody reactivity of a predominantly IgG1 type, leads to greater opsonisation of the respiratory burst in PMN, providing a mechanism for maintaining the inflammatory state in UC. (bmj.com)
Identification of a specific target antigen is a crucial step in mRNA vaccine development. (sigmaaldrich.com)
We offer tools for genomics analysis, protein analysis, and antibody screening techniques to provide a comprehensive approach to antigen discovery. (sigmaaldrich.com)
Among the bacterial serotypes, O antigen specificity is determined by the sugar sequence of the polysaccharide chains. (differencebetween.com)
O antigen and H antigen are two types of antigens used to determine the different serotypes of bacterial species. (differencebetween.com)
Identification of such tumor-associated cell surface antigen polypeptides, i.e. tumor associated antigens (TAA), allows specific targeting of cancer cells for destruction via antibody-based therapies. (justia.com)
Here we describe the characterization of the Aspergillus-specific mAb AP3 (IgG1κ), including the precise identification of its corresponding antigen. (olmdiagnostics.com)
An antibody is a protein produced by B lymphocyte cells that recognizes a particular foreign antigen, and thus triggers the immune response. (uhthoff.com.mx)
Recognition requires the antibody to bind to a small region or structure on the antigen. (uhthoff.com.mx)
All antibody applications, especially those that use monoclonal antibodies (mAbs), depend on antibodies' ability to form specific and selective bonds with a given epitope on the surface of an antigen. (uhthoff.com.mx)
As discussed above, the area of specific antigen identification remains relatively open, providing encouragement for the development of more directed targeting approaches. (johnshopkins.edu)

Monoclonal Antibody AP3 Binds Galactomannan Antigens Displayed by the Pathogens Aspergillus flavus, A. fumigatus, and A. parasiticus. (olmdiagnostics.com)
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. (bdbiosciences.com)
However, a monoclonal antibody (mAb) may be patented if, for example, it proves useful for producing a therapeutic response, provided the effect produced is not obvious (for the purposes of patentability). (uhthoff.com.mx)
Outcome Comparison of High-Risk Native American Patients Who Did or Did Not Receive Monoclonal Antibody Treatment for COVID-19. (upenn.edu)
TLR4/MD-2 monoclonal antibody therapy affords protection in experimental models of septic shock. (tum.de)

Application Instructions These antibodies have been qualified for use in ELISA to detect Group A Streptococci. (qedbio.com)
An enzyme-linked immunosorbent assay (ELISA) has been developed to quantitate levels of antibody specific for Erysipelothrix rhusiopathiae in the cetacean species Tursiops truncatus , Lagenorhynchus obliquidens , and Delphinapterus leucas . (vin.com)
1995. A biotin-avidin amplified ELISA for the detection of antibodies against Erysipelothrix rhusiopathiae in captive dolphins. (vin.com)
In May 2020, ERBA Diagnostics Mannheim GmbH (Germany) launched its immunoassay-based kit-ErbaLisa COVID-19 ELISA kits-to detect IgG and IgM antibodies against SARS-CoV-2. (thinkingcapsrc.com)
aCL antibody ELISA: IgG and IgM aCL antibodies were detected with an in-house previously explained ELISA. (oaxacalibre.net)
a2GP1 antibody ELISA: IgG and IgM anti-2GP1 antibodies were detected by using a commercially available ELISA (Orgentec Diagnostika GmbH, Mainz, Germany). (oaxacalibre.net)

In the case of immunosensors, this specificity is imparted by antibodies. (usda.gov)
Antibody phage display is a molecular method for the selection of antibody fragments of desired specificity and is an alternative to traditional means for the generation of polyclonal or monoclonal antibodies. (usda.gov)
Single chain antibody fragments (scFvs) of desired specificity are selected from a large collection of bacteriophage expressing unique scFvs through the binding of the phage displayed scFv with the target of interest. (usda.gov)
Specificity These antibodies recognize Group A Streptococci. (qedbio.com)
This review presents recent advancements in serodiagnostics and prevention of leishmaniasis -an important tropical parasitic disease- achieved using phage display for the identification of novel antigens with improved sensitivity and specificity. (scielo.org)
This selective specificity is also key in determining the patentability of an invention involving antibodies. (uhthoff.com.mx)
Specificity This antibody recognizes human and mouse CXCR4 (40 kD). (qedbio.com)
Culture testing is the criterion standard for B pertussis infection, owing to its high specificity (100%) for identification. (medscape.com)

The severely immune-compromised patient is prone to fungal as well as bacterial blood stream infections. (hindawi.com)
[ 2 ] Therefore, specific infections in the oral cavity may create foci of infection that may affect systemic health. (medscape.com)
One of the most common chronic bacterial oral infections, periodontitis, affects the supporting structures of the teeth. (medscape.com)
Specific medications used to treat infections include antibiotics , antivirals , antifungals , antiprotozoals , [3] and antihelminthics . (wikipedia.org)
As bacterial and viral infections can both cause the same kinds of symptoms, it can be difficult to distinguish which is the cause of a specific infection. (wikipedia.org)
[12] Distinguishing the two is important, since viral infections cannot be cured by antibiotics whereas bacterial infections can. (wikipedia.org)
Infections due to certain strains of S. pyogenes can be associated with the release of bacterial toxins. (qedbio.com)
Bacterial infections, such as salmonellosis, can also be traced back to these rodents. (insecta-inspecta.com)
Phage display has provided unmatched tools for controlling viral, bacterial, fungal, and parasitic infections, and allowed identification of new therapeutic targets to treat cancer, metabolic diseases, and other chronic conditions. (scielo.org)
The ISU-VDL has recently developed a novel M. hyorhinis indirect enzyme-linked immunosorbent assay based on a recombinant chimeric made of variable lipoproteins specific (unique) to M. hyorhinis that allow for early and differential detection of M. hyorhinis infections with no cross-reactivity with other swine mycoplasmas. (nationalhogfarmer.com)
These findings will contribute to fish immunotherapy for the prevention and treatment of bacterial infections through the design of more specific and effective immune stimulants, adjuvants, and vaccines. (frontiersin.org)
Felix d'Herelle first coined the term "bacteriophage" ( 4 ), literally meaning "bacterium eater," and began using phages to treat bacterial infections in human patients ( Fig. 1 ). (asm.org)
The patients have experienced recurring infections, including bacterial infections of the respiratory system and chronic viral infections with EBV, since childhood, and some have developed EBV-associated lymphoma. (nih.gov)
ABSTRACT Neisseria meningitidis, a leading cause of bacterial meningitis and other serious infections, is responsible for approximately one-third of cases of bacterial meningitis in the Children's Hospital of Tunis. (who.int)
Patients with antibody deficiencies are especially susceptible to encapsulated bacteria, which cause pyogenic infections. (lu.se)

Immunosensor-based detection methods are available for a number of food-borne pathogens, but, until recently, their application to the detection of L. monocytogenes has been hampered by the lack of species-specific polyclonal serum or monoclonal antibodies. (usda.gov)
Moreover, unlike a polyclonal antibody, if the mAb normally exhibits a therapeutic effect that is most reproducible in a particular type of patient, this characteristic of the mAb may contribute to the patentability of the invention. (uhthoff.com.mx)
and / or polyclonal antibodies specific to a particular target for capture and / or quantitative detection. (basepairbio.com)

The progressions of M. hyorhinis clinical disease (lameness and loss of condition) were evaluated in relation to the kinetics of the isotype-specific (IgG, IgA) antibody response in serum and bacterial shedding (PCR) in oral fluids using a cesarean-derived, colostrum-deprived pig model. (nationalhogfarmer.com)
IgA antibody was first detected at DPI 7 concomitantly with the onset of clinical signs, with both IgA and IgG detected in all serum samples collected after DPI 14 through the end of the study. (nationalhogfarmer.com)
and 11) D. leucas serum with anti- E. rhusiopathiae antibodies removed. (vin.com)

Antibodies to CXCR4 may block HIV-1 and HIV-2 infection of human target cells. (qedbio.com)
Overactivation of the immune system upon acute bacterial infection leads to septic shock. (tum.de)
These results demonstrate that neutralizing TLR4/MD-2 is highly efficacious in protecting against bacterial infection-induced toxemia and offers TLR4/MD-2 mAb treatment as a potential therapy for numerous clinical indications. (tum.de)
Prior to developing the condition, most people with Guillain-Barré syndrome have a bacterial or viral infection. (medlineplus.gov)
Using the complement fixation test as the gold standard for confirmatory tests, the Rivanol test was found to be more sensitive but less specific than tube agglutination in detecting brucellosis infection. (who.int)

Relative to traditional microbiological approaches, biosensors are a rapid method for foodborne bacterial pathogen detection. (usda.gov)
The anti-L. monocytogenes IMB capture was combined with microbiological and PCR methods for specific detection of L. monocytogenes. (usda.gov)
This study demonstrates the applicability of antibody fragments selected via phage display for the development of immunoreagents and the detection of foodborne pathogens. (usda.gov)
The cysteine engineered antibodies may be conjugated with chemotherapeutic drugs, toxins, affinity ligands such as biotin, and detection labels such as fluorophores. (justia.com)
Tests that have generated interest include combined detection of mannan and anti-mannan antibodies, β -1, 3, D-glucan detection, and a number of molecular techniques. (hindawi.com)
Detection of abrin holotoxin using novel monoclonal antibodies //Toxins. (medline.ru)
The target protein is then bound by a labeled antibody for fluorescent or colorimetric detection. (basepairbio.com)
Unfortunately, antibody-based dot blot assays are unstable at high temperatures and are optimized for detection in biological samples. (basepairbio.com)

Cysteine engineered anti-MUC16 antibodies (Ab) are conjugated with one or more drug moieties (D) through a linker (L) to form cysteine engineered anti-MUC16 antibody-drug conjugates having Formula I: Ab-(L-D)p I where p is 1 to 4. (justia.com)
The use of antibody-drug conjugates (ADC), i.e. immunoconjugates, for the local delivery of cytotoxic or cytostatic agents, i.e. drugs to kill or inhibit tumor cells in the treatment of cancer (Lambert, J. (2005) Curr. (justia.com)
Drug moieties used in antibody drug conjugates include bacterial protein toxins such as diphtheria toxin, plant protein toxins such as ricin, small molecules such as auristatins, geldanamycin (Mandler et al (2000) J. of the Nat. (justia.com)
The guaranteed shelf life from date of receipt for antibodies and conjugates is listed on the product information sheet. (biotium.com)
Antibodies and other conjugates often are functional for significantly longer than the guaranteed shelf life. (biotium.com)
For lyophilized antibodies, we recommend reconstituting the antibody with glycerol and antimicrobial preservative like sodium azide for the longest shelf life (note that sodium azide is not compatible with HRP-conjugates). (biotium.com)

The ANCA assay (INOVA) detects the presence of IgG antibodies that bind to human neutrophil antigens using direct immunofluorescence.¹,² Screening all samples with ethanol fixed slides allows ANCA reactivity to be separated into two diagnostically useful categories. (nemours.org)

Systemic antibody responses were measured against total antigens and surface antigens of these organisms in UC and Crohn's disease (CD) patients, together with healthy controls. (bmj.com)
Antigens are generally used in serotype identification system for the bacteria. (differencebetween.com)
Widal test measures antibodies against H and O antigens. (differencebetween.com)
Rabbit anti Treponema pallidum antibody recognizes all Treponema pallidum antigens. (bio-rad-antibodies.com)
Many commercially available tests for detecting SARS-CoV-2-specific antigens have recently been developed. (thinkingcapsrc.com)
The STANDARD F COVID-19 Ag fluorescence immunoassay (FIA) kit is also available, which uses a fluorescent immunoassay to detect specific nucleoprotein antigens to SARS-CoV-2 in the human nasopharynx. (thinkingcapsrc.com)
The immunofluorescence ANA assay (Antibodies, Inc) utilizes the method considered the gold standard for ANA testing by the American College of Rheumatology: detecting circulating autoantibodies reactive with nuclear antigens of HEp-2 cells. (nemours.org)

Antibodies from UC patients greatly enhanced the respiratory burst in PMN, in response to individual bacterial species. (bmj.com)
A species-specific quantitative PCR was designed, and detected the organism within the liver and colon of the index case. (21docs.com)
The antibody was generated using A. parasiticus cell wall fragments and was shown to bind several Aspergillus species. (olmdiagnostics.com)
The prevalence of specific diseases can also vary among species. (insecta-inspecta.com)
The DEGs in different fish species were also representative of specific immune-related pathways. (frontiersin.org)

While the project achieved many of its original objectives as outline above, despite several attempts we were not able to produce reliable recombinant antibodies [using phage display technology] for our selected biomarker panels. (europa.eu)

21:183-87) and monoclonal antibodies (mAbs) as well as drug-linking and drug-releasing properties (Lambert, J. (2005) Curr. (justia.com)
However, the use of monoclonal antibodies (mAbs) for the diagnosis of IA is often associated with false positives due to cross-reaction with bacterial polysaccharides. (olmdiagnostics.com)
An antibody may also be eligible for patent protection if, during the research phase, there was no reasonable expectation of success in obtaining mAbs with the desired specific characteristics pre¬sented by that antibody. (uhthoff.com.mx)

Antibody phage display involves the cloning of nucleotide sequences encoding the antibody variable regions and display of the antibody fragments as fusion proteins on the surface of bacteriophage. (usda.gov)
We isolated a L. monocytogenes-specific scFv from a phage display library. (usda.gov)
It is based on the selection of phage-fused surface-exposed peptides that recognize specific ligands and demonstrate desired functionality for diagnostic and therapeutic purposes. (scielo.org)
Temperate phages are capable of protecting their host from phage reinfection and may change the bacterial phenotype through the expression of viral genes, a process known as lysogenic conversion ( 1 ). (asm.org)

Monitoring of laying capacity, immunoglobulin Y concentration, and antibody titer development in chickens immunized with ricin and botulinum toxins over a two-year period // Poult. (medline.ru)
1) Antibody deficiency disorders are defects in immunoglobulin-producing B cells . (lu.se)
Antibody deficiencies are treated with intravenous immunoglobulin substitution therapy. (lu.se)

GAS' M protein constitutes a primary target of the immune system due to its spatial configuration and dominance on the bacterial surface. (lu.se)
Antibody responses targeting the M protein have been shown to favor the conserved C region. (lu.se)
Some cytotoxic drugs tend to be inactive or less active when conjugated to large antibodies or protein receptor ligands. (justia.com)
Immunohistochemistry using an antibody directed against a Neorickettsia surface protein showed bacterial clusters within the renal granulomas. (21docs.com)
The DCN46 antibody specifically binds to dendritic cell-specific ICAM-3 grabbing nonintegrin (DC-SIGN or CD209), a type-II membrane protein of approximately 44 kDa with a mannose-binding C-type lectin domain. (bdbiosciences.com)
In view of the fact that sequencing of genomes is usually considered straightforward, patent offices around the world in many cases consider the identification of a new protein as obvious and non-patentable. (uhthoff.com.mx)
Only in exceptional cases where the identification of the protein has faced considerable difficulties may it still be possible to obtain patent protection for a naturally occurring protein (or at least for a specific epitope). (uhthoff.com.mx)

Optimal dilution for a specific application should be determined. (biotium.com)
mouse liver tissue were subjected to SDS PAGE followed by western blot with 19811-1-AP (TLR4 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. (ptglab.com)
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 19811-1-AP (TLR4 Antibody) at dilution of 1:200 (under 10x lens). (ptglab.com)
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 19811-1-AP (TLR4 Antibody) at dilution of 1:200 (under 40x lens). (ptglab.com)

Plasmids purified from genomic DNA, proteins, ribosomes, and the bacterial cell wall are used in molecular biology research. (sigmaaldrich.com)
PI3K proteins are essential for directing the growth and activity of many types of immune cells, and p110 delta is specifically involved in B and T cells, which make antibodies and recognize and eliminate infected cells, respectively. (nih.gov)

Determination of specific antibodies to viral and bacterial pathogens and parasites facilitates the correct therapeutic measures. (thinkingcapsrc.com)

Aptamers as a replacement for antibodies in enzyme-linked immunosorbent assay. (basepairbio.com)

The aim of this investigation was to compare the principal culturable bacterial populations on the rectal mucosa of UC patients, and to determine whether specific antibodies towards these bacteria can activate infiltrating PMN through opsonisation. (bmj.com)

While antibody-based immunoassays have proven to be very sensitive and specific, there are some limitations which can be overcome with the ELASA, or Enzyme-Linked Aptamer Sorbent Assay. (basepairbio.com)

We examined hepatocellular carcinoma (HCC) development in alcohol Western diet-fed hepatitis C virus NS5A Tg mice with hepatocyte-specific TBC1D15 deficiency or expression of non-phosphorylatable NUMB mutations. (nature.com)
Liver-specific TBC1D15 deficiency or non-p-NUMB expression reduced TIC numbers and HCC development. (nature.com)

Group A streptococcus (GAS) is a highly adapted, human-specific pathogen that is known to manipulate the immune system through various mechanisms. (lu.se)

In order to elucidate the mechanisms by which these compounds antagonize Salmonella biofilms, in Aim 2 we will identify the specific bacterial target(s) of each compound by enriching for mutants exhibiting compound resistance and by performing direct pull-downs of targets from bacterial lysates. (hhs.gov)

Binds bacterial lipopolysaccharide (LPS) et mediates LPS-induced inflammatory response, including TNF secretion by monocytes (PubMed:11276205). (qedbio.com)

Transmembrane or otherwise tumor-associated polypeptides specifically expressed on the surface of cancer cells as compared to normal, non-cancerous cell(s) have been identified as cellular targets for cancer diagnosis and therapy with antibodies. (justia.com)
This antibody has not been absorbed and may react with related targets. (bio-rad-antibodies.com)
DESCRIPTION (provided by applicant): Identification and Validation of Targets of Phenotypic High Throughput Screening Hits for Chagas Disease Project Summary Nearly 10 million people in Latin America are infected with the eukaryotic parasite Trypanosoma cruzi, the causative agent of Chagas disease. (sbir.gov)
Like monoclonal antibodies, aptamers offer both selectivity and affinity for specific targets. (basepairbio.com)

Follow specific protocols that apply (e.g. (fpnotebook.com)

A mixed sample is passed over the support and the target is separated through specific interactions with the affinity ligand. (basepairbio.com)

The invention also relates to methods of using antibodies and antibody-drug conjugate compounds for in vitro, in situ, and in vivo diagnosis or treatment of mammalian cells, or associated pathological conditions. (justia.com)
An ideal platform must offer quick, specific diagnosis, be economical, and have minimum hands-on time. (hindawi.com)
A diagnosis of M. hyorhinis -associated disease is made based on history, clinical signs, typical gross and microscopic lesions, and isolation of M. hyorhinis by culture in specific media (i.e. (nationalhogfarmer.com)
For a more rapid diagnosis, direct immunofluorescent antibody (DFA) testing on nasopharyngeal smears may be an option. (medscape.com)

Diagnostic and therapeutic uses for cysteine engineered antibody drug compounds and compositions are disclosed. (justia.com)
Unlike antibodies, aptamers can be selected for specific binding to poorly immunogenic and toxic compounds. (basepairbio.com)

Using random hexamers priming, reverse transcriptase (RT) convert all mRNA into cDNA and cloned into bacterial vectors. (wikipedia.org)

Adaptive immune mechanisms recognize and neutralize foreign molecules or microorganisms in a specific manner. (lu.se)

The invention relates generally to antibodies engineered with reactive cysteine residues and more specifically to antibodies with therapeutic or diagnostic applications. (justia.com)

Acute termination of viral hepatitis serologies are detectable by fish, - mb bacterial artificial chromosome arrays that can be achieved with the episode. (albionfoundation.org)

Technology making the transfusion of allogeneic blood products feasible includes Karl Landsteiner's landmark identification of the human blood groups A, B, and O in 1901. (medscape.com)

ML could be the next step of personalized medicine in chronic obstructive pulmonary disease (COPD) by giving the exact risk (risk for exacerbation, death, etc.) of every patient (based on his/her parameters like lung function, clinical data, demographics, previous exacerbations, etc.), thus providing a prognosis/risk for the specific patient based on individual characteristics (individu-al approach). (imperial.ac.uk)

Pan-bacterial 16S rRNA gene PCR and amplicon sequencing of affected tissue indicated the presence of a Neorickettsia . (21docs.com)

Salmonella is a bacterial genus that has many serotypes. (differencebetween.com)
Towards this end, our group has identified four small molecules and two antibodies capable of inhibiting and/or disrupting Salmonella biofilms in vitro. (hhs.gov)

Such antibodies (Abs) circumvent antigenic escape and efficiently bind to various M types. (lu.se)

Methods of design, preparation, screening, and selection of the cysteine engineered anti-MUC16 antibodies are provided. (justia.com)
Uhlenbeck( OU) download and its matrices, is to estimate otherwise F2 in specific and Average authors expressing review and variability sites in site to isolation methods of outcomes. (scoutconnection.com)

Distinct differences were observed in some bacterial populations in UC biopsies, which were generally reflected in antibody responses towards these organisms. (bmj.com)

Future immunotherapy approaches for prostate cancer will almost certainly combine active immunotherapy with either antitumor monoclonal antibodies or with coinhibitor blockade to augment efficacy. (johnshopkins.edu)

Anatomy22

Organisms22

Diseases5

Chemicals and Drugs112

Analytical, Diagnostic and Therapeutic Techniques and Equipment54

Phenomena and Processes46

Disciplines and Occupations2

Information Science3

Health Care5

Antigen12

Monoclonal Antibody5

ELISA6

Specificity8

Infections15

Polyclonal3

Serum3

Infection5

Detection8

Conjugates6

Reactivity1

Antigens7

Species5

Recombinant1

MAbs3

Phage4

Immunoglobulin3

Protein7

Dilution4

Proteins2

Pathogens1

Enzyme-linke1

Bacteria1

Immunoassays1

Deficiency2

Immune system1

Lysates1

Binds1

Targets4

Protocols1

Affinity1

Diagnosis4

Compounds2

Vectors1

Recognize1

INVENTION1

Acute1

Human1

Chronic1

RRNA1

Salmonella2

Bind1

Methods2

Responses1

Immunotherapy1