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  • residue
  • A) clustalw nongapped multiple sequence alignment of ankyrin repeat sequences containing a target histidine residue at the conserved hydroxylation position. (nih.gov)
  • To investigate whether FIH-catalysed His hydroxylation can occur in other AR sequences, we searched for naturally occurring AR sequences containing an 'LxxxxxDVH' motif with the His residue located at the conserved hydroxylation position, and tested the corresponding peptides as FIH substrates (Fig. 6). (nih.gov)
  • vitro
  • Hydroxylation-deficient AKT mutants induced enhanced anchorage-independent growth in vitro and tumor formation in vivo compared with wild-type AKT, demonstrating that hydroxylation of AKT modulates oncogenic signaling. (aacrjournals.org)
  • Corresponding peptides spanning the potential histidinyl hydroxylation sites were tested as FIH substrates in vitro, among which peptides derived from TNKS1, GABPB2 and TRPV4 demonstrate FIH-dependent hydroxylation. (nih.gov)
  • As a first step in substantiating this hypothesis, we have developed and validated an in vitro propofol hydroxylation system, which incorporates a novel and highly sensitive 4-hydroxypropofol assay using HPLC with fluorescence detection. (aspetjournals.org)
  • pathway
  • Taken together, these results indicate that hypoxia and defects in the VHL-EGLN pathway lead to hydroxylation-dependent hyperactivation of AKT, and suggest that targeting the AKT pathway may be effective in patients with VHL-deficient tumors as well as more generally in hypoxic tumors. (aacrjournals.org)
  • Whereas 4-hydroxylation constitutes the minor pathway in the liver, the greater proportion of CYP1B1 expression in extrahepatic tissues shifts the balance in favor of 4-OH-E2 formation. (wikipedia.org)
  • prediction
  • In this paper, we propose pool-based active learning with support vector machine (SVM) classifiers for the prediction of asparagine/aspartate (N/D) hydroxylation sites on proteins. (actapress.com)
  • Using a dataset of N/D sites with known hydroxylation state, we here demonstrate through simulations that active learning query strategies can achieve higher classification performance with fewer labelled training instances for hydroxylation site prediction, compared to traditional passive learning. (actapress.com)
  • minor
  • This graph shows the total number of publications written about "Hydroxylation" by people in Harvard Catalyst Profiles by year, and whether "Hydroxylation" was a major or minor topic of these publication. (harvard.edu)
  • sites
  • The active learning procedure could therefore be used to choose which putative hydroxylation sites should be selected for future wet-lab experimental validation and verification in order to gain maximal information. (actapress.com)
  • MS/MS analyses assigned sites of hydroxylation to His 711 in the tankyrase-1 sequence (Fig. S3) and His 265 in the TRPV4 sequence (Fig. S4), both of which are located within the β-hairpin loop at the structurally conserved hydroxylation position. (nih.gov)
  • The present study could shed some light on the essence and function of active sites on carbon for the benzene hydroxylation to phenol. (rsc.org)
  • site
  • Site-directed mutagenesis combined with structural and functional analyses indicated that hydrophobic interactions and shielding effects against water are important factors in the hydroxylation of biphenyl by the o-xylene dioxygenase. (nih.gov)