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Chromatography, AffinityChromatographyChromatography, AgaroseChromatography, Ion ExchangeChromatography, GelHydrophobic and Hydrophilic InteractionsChromatography, High Pressure LiquidAmmonium SulfateChromatography, LiquidMolecular WeightElectrophoresis, Polyacrylamide GelPolymerase Chain ReactionMolecular Sequence DataAmino Acid SequenceHydrogen-Ion ConcentrationKineticsClinical Laboratory TechniquesIsoelectric PointSubstrate SpecificityEnzyme StabilityIsoelectric FocusingSolubilityAmino AcidsAcetonitrilesTemperatureEscherichia coliSurface PropertiesChromatography, GasGlycomicsChromatography, Thin LayerGelsSepharoseMass SpectrometryTandem Mass SpectrometryProtein BindingLaboratoriesCattleSpectrometry, Mass, Electrospray IonizationBinding SitesModels, MolecularPolysaccharidesChromatography, DEAE-CelluloseProtein ConformationRecombinant ProteinsThermodynamicsProtein Structure, SecondaryBase SequenceReproducibility of ResultsCircular DichroismPeptide FragmentsPeptidesStructure-Activity RelationshipChemistryChemical PhenomenaProtein Structure, TertiaryMacromolecular SubstancesCarbohydratesLectinsSequence Homology, Amino AcidMagnetic Resonance SpectroscopyRabbitsSpectrophotometry, UltravioletGas Chromatography-Mass SpectrometryHydrogen BondingPhysicochemical PhenomenaCloning, MolecularBinding, CompetitiveCrystallography, X-RayChemistry, PhysicalMolecular StructureBacterial ProteinsChromatography, Reverse-PhaseCarrier ProteinsTrypsinAdsorptionAffinity LabelsLigandsSolventsHot TemperatureChromatography, PaperHydrolysisProtein DenaturationDetergentsProtein FoldingSpectrometry, FluorescenceCell MembraneGlycoproteinsCell LineLiverCarbohydrate SequenceStatic ElectricityOligosaccharidesMutationDrug StabilityProteinsMutagenesis, Site-DirectedWaterImmunodiffusionRecombinant Fusion ProteinsDimerization
ResinsHPLCProteinsChromatographic techniqueMultimodalPreparativeAgaroseSeparationColumnsPurificationSuitableLigandsPolymersAnalyticalStationary phaseSilica gelEluteConsumablesHydrophobicityCellulosePhosphorylationMolecularTarget moleculesChemicalResinMolecules basedColumnMechanismPostdoctoral positionBehaviorCompoundsFluorescenceScientificLiquidMethodsAnalysisSizeOutlookCompoundSpecificSystemProcessPropertiesEnzymeApplicationsDistinctHighSeparatePhasesWash
Resins3
  • This offer lets you purchase filter plates and columns pre-packed with a range of process chromatography resins. (excedr.com)
  • Another factor driving the adoption rate of protein purification techniques is the availability of an extensive range of innovative resins for various applications. (radiantinsights.com)
  • Separation technique in which the stationary phase consists of ion exchange resins. (lookformedical.com)
HPLC1
Proteins8
  • This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins. (sigmaaldrich.com)
  • This page describes efficient column packing and preparation for affinity chromatography of tagged proteins using Cytiva products. (sigmaaldrich.com)
  • This page shows how to separate proteins and peptides with affinity for metal ions by immobilized metal chelate affinity chromatography using HiTrap Chelating HP, Chelating Sepharose Fast Flow,His MicroSpin Purification Module or HisTrap Kit from Cytiva. (sigmaaldrich.com)
  • Recombinant proteins commonly incorporate N- and C-terminal tags, which are small sequences that are used for immunohistochemical detection and purification, or protein affinity chromatography, depending on the specific N- and C-terminal tag and intended downstream application. (sigmaaldrich.com)
  • however, unwanted proteins are likely to also bind to the affinity resin column and will elute in the final wash along with the desired protein of interest. (sigmaaldrich.com)
  • Plant proteomics currently has moved from traditional gel-based strategies in which many low abundant, extreme pI and hydrophobic proteins were underrepresented, to gel-free shotgun workflows. (frontiersin.org)
  • One common technique for plasmid purification is the alkaline lysis method, which breaks open bacteria with an alkaline solution, proteins are removed by precipitation and the plasmid DNA is recovered with alcohol precipitation. (gbiosciences.com)
  • Size Exclusion Chromatography or gel filtration is a widely used technique for separation of proteins and other molecules based on their sizes. (gbiosciences.com)
Chromatographic technique2
  • For a full assessment of the purity of a sample, different chromatographic separation procedures are used so that any components not resolved by one procedure are likely to be resolved based on another chromatographic technique. (biopharmaspec.com)
  • A chromatographic technique that utilizes the ability of biological molecules to bind to certain ligands specifically and reversibly. (lookformedical.com)
Multimodal1
Preparative12
Agarose3
  • Agarose bound Ricinus communis agglutinin I is prepared using our affinity-purified lectins. (vectorlabs.com)
  • 1) Draw (pipet) the desired amount of settled agarose-lectin (gel) from the stock bottle into the prepared column and let the buffer drain by gravity. (vectorlabs.com)
  • A method of gel filtration chromatography using agarose, the non-ionic component of agar, for the separation of compounds with molecular weights up to several million. (lookformedical.com)
Separation2
  • In the downstream separation and purification process of biopharmaceuticals, chromatography is one of the indispensable separation methods. (biolink.com)
  • When double-stranded DNA (dsDNA) is heated, interaction forces that contribute to the formation of double strand such as hydrogen bonding between the bases pairs is weakened, resulting in the separation of dsDNA into two single-stranded DNA (ssDNA). (jascoinc.com)
Columns5
  • In addition, Bio-Link also produces chromatography columns and prepacked columns from laboratory to industrial grade. (biolink.com)
  • TSKgel Butyl-NPR hydrophobic interaction columns for analysis of antibody-drug conjugates (ADCs) and as an orthogonal method to analyze protein aggregation, oxidation and deamidations. (selectscience.net)
  • TSKgel Protein A-5PW affinity columns for direct analysis of mAb titer. (selectscience.net)
  • TSKgel FcR-IIIA-NPR affinity columns for direct analysis of mAb activity. (selectscience.net)
  • Chromatography on thin layers of adsorbents rather than in columns. (lookformedical.com)
Purification3
  • If additional purification is necessary, supplementary purification strategies are employed including, size-exclusion chromatography or ion exchange chromatography. (sigmaaldrich.com)
  • Hydrophobic chromatography is a chromatographic mode for purification based on the difference in hydrophobicity between the target product and impurities. (biolink.com)
  • About a 40-fold purification of the cyclase supernatant protein was achieved by a combination of (NH4)2SO4 fractionation and phenyl-Sepharose chromatography. (docksci.com)
Suitable2
Ligands3
  • Since the hydrophobic characteristics of the target product are difficult to obtain directly, when designing a hydrophobic chromatography process, pre-experiments should be carried out with fillers containing different hydrophobic ligands. (biolink.com)
  • Apomyoglobin (apoMb), a model protein in biochemistry , exhibits a strong propensity to bind various ligands, which makes it a good candidate as a carrier of bioactive hydrophobic drugs. (cea.fr)
  • The cysteine engineered antibodies may be conjugated with chemotherapeutic drugs, toxins, affinity ligands such as biotin, and detection labels such as fluorophores. (justia.com)
Polymers3
Analytical2
  • In analytical chromatography, the goal is to separate and uniquely identify each of the compounds in a substance. (wikipedia.org)
  • An analytical technique for resolution of a chemical mixture into its component compounds. (lookformedical.com)
Stationary phase4
  • This technique separates molecules using the interaction of the protein, glycoprotein or peptide with a hydrocarbon coated stationary phase. (biopharmaspec.com)
  • This is achieved through the association of exposed hydrophobic domains within the protein with the stationary phase as a high salt buffer. (biopharmaspec.com)
  • Two types are gas-solid chromatography, where the fixed phase is a solid, and gas-liquid, in which the stationary phase is a nonvolatile liquid supported on an inert solid matrix. (lookformedical.com)
  • It is commonly used as a desiccating agent and as a stationary phase for CHROMATOGRAPHY. (lookformedical.com)
Silica gel3
  • YMC-Pack Diol is a size exclusion chromatography column utilizing a silica gel base. (thomassci.com)
  • LiChrosorb® RP-8 is a reliable irregular shaped silica gel carriers with reversed phase properties. (thomassci.com)
  • The fully hydrated form of silica gel has distinct properties and is referred to as SILICIC ACID. (lookformedical.com)
Elute1
  • A decreasing salt gradient is used to elute components, with more hydrophobic species eluting with lower salt concentrations. (biopharmaspec.com)
Consumables2
  • Bio-Rad Laboratories, a developer and manufacturer in the life sciences industry, provides instruments, apparatus, software, consumables, and other materials necessary to assist the life science research and clinical diagnostics markets. (excedr.com)
  • Besides equipment, Bio-Rad also provides kits, reagents, and consumables often needed by life science laboratories , such as flow cytometry antibodies, ELISA reagents, and even SARS-CoV-2 ddPCR kits . (excedr.com)
Hydrophobicity2
  • The hydrophobicity of the hydrophobic ligand butyl-octyl-phenyl increases sequentially, and the hydrophobicity of the target product can be predicted through the combined adsorption test with different hydrophobic fillers. (biolink.com)
  • The binding affinity of OBP2a to fatty aldehydes including hexanal, heptanal, 2-octenal, decanal, and β-cyclocitral increased with the increase of hydrophobicity of aldehydes. (springeropen.com)
Cellulose2
  • western transfer and immunoenzymatic staining with affinity-purified monospecific antiserum was used to detect a 38-kd protein that bound to native and denatured dna cellulose. (liverpool.ac.uk)
  • A type of ion exchange chromatography using diethylaminoethyl cellulose (DEAE-CELLULOSE) as a positively charged resin. (lookformedical.com)
Phosphorylation3
  • An in vitro binding kinetic analysis revealed that these phosphorylation-dependent events were attributable to a decrease in the affinity of phosphorylated Homer3 for its ligand. (jneurosci.org)
  • In this study, we revealed that Homer3 was phosphorylated by calcium/calmodulin-dependent protein kinase II (CaMKII) in Purkinje cells, and the phosphorylation reduces the affinity for Homer target molecules, resulting in change of the solubility. (jneurosci.org)
  • FLIM-FRET analysis of protein-protein interactions showed that PLIN5 S155 phosphorylation regulates PLIN5 interaction with adipose triglyceride lipase at the lipid droplet, but not with α-β hydrolase domain-containing 5. (uci.edu)
Molecular6
  • The main motivation here is to make advances in our understanding of molecular mechanisms of T cell function and its interaction with the tumor. (vacancyedu.com)
  • In a molecular interaction and complex formation, it is considered to be enthalpy-driven when the effect of negative ∆H° on ∆G° is larger than that of negative ∆S° , on the other hand, when the effect of positive ∆S° on ∆G° overcomes that of positive ∆H° , it is considered to be entropy-driven (Fig. 3). (jascoinc.com)
  • Molecular dynamics (MD) simulations showed a preferred interacting crystallographic plane for both crystalline allomorphs that exposes the CH groups (100 and 010) and is therefore considered hydrophobic. (cea.fr)
  • In this study, the binding affinity and mechanism of human odor-binding protein OBP2a to 14 typical odorants in water were first assessed using fluorescent competitive binding assays and molecular docking techniques. (springeropen.com)
  • The test derivatives were additionally docked for the B-subunit of enzyme DNA gyrase from E. coli at the ATPase binding site to study the molecular interactions using Schrodinger maestro v11.5 software. (biomedcentral.com)
  • Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. (lookformedical.com)
Target molecules2
  • Oligonucleotide therapeutics are mainly based on artificial oligonucleotides that are chemically modified DNA or RNA, which improves their structural stability in the human body and affinity to their target molecules 1 ). (jascoinc.com)
  • The evaluation of their interaction characteristics with target molecules and use as drugs, optimization of their number of the bases and sequence are critical. (jascoinc.com)
Chemical2
  • It is well known that there is a correlation between enthalpy change ( ∆H° ) and entropy change ( ∆S °) in most chemical and biological interactions. (jascoinc.com)
  • Since plastics are known to sorb hydrophobic organic contaminants (HOCs) there is a question of what risk of chemical exposure is posed to aquatic biota from microplastic-associated contaminants. (researchgate.net)
Resin2
Molecules based1
  • This technique separates molecules based on their overall charge. (biopharmaspec.com)
Column4
  • This page discusses column packing and preparation techniques for reverse phase chromatography. (sigmaaldrich.com)
  • During SEC, molecules are separated based on size as they pass through a gel column composed of beads with a defined pore size. (biopharmaspec.com)
  • After use, wash the gel with several column volumes of buffered saline, then resuspend gel in buffered saline containing 0.08% sodium azide for storage. (vectorlabs.com)
  • 2) Wash the gel with 10 column volumes of buffer, such as HBS (10 mM HEPES, 0.15 M NaCl, pH 7.5) and discard the flow through. (vectorlabs.com)
Mechanism2
  • Chromatography on non-ionic gels without regard to the mechanism of solute discrimination. (lookformedical.com)
  • Laboratory tests for evaluating the individual's clotting mechanism. (lookformedical.com)
Postdoctoral position2
  • I am applying for the postdoctoral position in your Laboratory. (vacancyedu.com)
  • I subsequently took up a postdoctoral position in the Laboratory of Biophysics and Surface Analysis (LBSA), School of Pharmacy, University of Nottingham under the direction of Martyn Davies, Saul Tendler and David Jackson. (nottingham.ac.uk)
Behavior1
Compounds5
  • The stability of its hydrophobic pocket determines its potential as a carrier of bioactive compounds. (cea.fr)
  • Compounds 1-5 showed -12.53 to -10.11 kcal/mol potential binding affinity with glucocorticoid receptor (GR) and good ADME profile. (hindawi.com)
  • we designed new compounds with better hydrophobic properties facing Phe357. (ommegaonline.org)
  • All designed compounds showed comparable affinity to the catalytic site of the enzyme to that of the standard drug, however, the activity as inhibitors, as reflected by the IC 50 range of 0.3 μM - 1.3 μM, was lower than that of Sitagliptin (IC 50 22 nM). (ommegaonline.org)
  • A microanalytical technique combining mass spectrometry and gas chromatography for the qualitative as well as quantitative determinations of compounds. (lookformedical.com)
Fluorescence2
  • By combining HP-fluorescence and HP-visible absorption spectroscopy , our strategy highlights the crucial role of tetrapyrrole-metal complexes in stabilising apoMb hydrophobic pocket. (cea.fr)
  • Articles, abstracts, and book chapters citing Globals Software (Globals Unlimited, Globals for Spectroscopy, and Globals for Images aka SimFCS) by authors not affiliated with the Laboratory for Fluorescence Dynamics (LFD). (uci.edu)
Scientific1
  • Bio-Rad aims to support scientific education, research, and discovery for laboratories across every industry. (excedr.com)
Liquid6
  • Reduce plastic waste and eliminate hazardous liquid waste for more sustainable laboratories with GenElute™-E Single Spin DNA and RNA prep kits. (sigmaaldrich.com)
  • Liquid chromatographic profiling is the use of chromatographic techniques to separate molecules by different physicochemical properties. (biopharmaspec.com)
  • Resistant to most solutions used in liquid chromatography except hydrocarbons, aromatic solvents and chlorinated hydrocarbons. (govsci.com)
  • Using ultrahigh-performance liquid chromatography-mass spectrometry, size exclusion chromatography is applied, and a virtual database of glycans in enoxaparin is established for the initial searching. (bvsalud.org)
  • Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed. (lookformedical.com)
  • Chromatographic techniques in which the mobile phase is a liquid. (lookformedical.com)
Methods1
  • However, we now have a number of methods that allow identification of genes critical for survival in a host as well as methods that allow direct measurement of gene expression during interaction with a host. (cdc.gov)
Analysis1
Size1
Outlook1
  • A Feature Paper should be a substantial original Article that involves several techniques or approaches, provides an outlook for future research directions and describes possible research applications. (mdpi.com)
Compound4
  • Computational studies were enrolled to predict the interaction of the synthesized compound with sarco-endoplasmic reticulum (SR) Ca 2+ transport ATPase (SERCA), a target of relevance for developing new therapeutics against arthritis. (mdpi.com)
  • The forces of interaction of the most active compound with the enzyme catalytic site were determined from modeling studies, binding can be optimized by further structural modifications. (ommegaonline.org)
  • Any compound containing one or more monosaccharide residues bound by a glycosidic linkage to a hydrophobic moiety such as an acylglycerol (see GLYCERIDES), a sphingoid, a ceramide (CERAMIDES) (N-acylsphingoid) or a prenyl phosphate. (lookformedical.com)
  • To know how inflammation is set up in reaction to bacteria it is essential to give attention to the primary common compound library interactions between the host cells and these, that will be performed by the innate immunity. (ilreceptor.com)
Specific1
  • High pressure (HP) is a potent tool for studying protein stability, revealing the specific role of hydrophobic cavities in unfolding. (cea.fr)
System4
  • Generally speaking, the target product will be combined with a hydrophobic filler in a high-salt system and eluted under a low-salt condition. (biolink.com)
  • X-ray structure of Sitagliptin with DPP-IV indicated hydrophobic interaction of the triazolo-piperazine ring system with the S2 pocket through the amino acid Phe357. (ommegaonline.org)
  • The current research aimed to design molecules maintaining the β-amino amide pharmacophore of Sitagliptin, but with the triazolo-piperazine ring system replaced by more lipophilic entities for better hydrophobic interactions with Phe 357 in the S2 pocket. (ommegaonline.org)
  • Given that these series of evaluations focus on host microbe interactions and based on the essential role played by the innate immune system in these events, we made a decision to emphasize the role of p38 MAPK signaling pathway in the innate immune response in the initiation of periodontal disease. (ilreceptor.com)
Process1
  • The process of the interaction of BLOOD COAGULATION FACTORS that results in an insoluble FIBRIN clot. (lookformedical.com)
Properties1
  • BioPharmaSpec has extensive experience in employing a comprehensive range of chromatographic techniques to separate molecules by different physicochemical properties. (biopharmaspec.com)
Enzyme1
  • In addition, we used XRD to determine the CNC crystallinity and observed no considerable difference between CNC-S and CNC-H. FT-IR has been used to characterize the functional groups on CNC-H and CNC-S. Zeta potential has been compared iii between CNC-S and CNC-H, which has a potential effect on electrostatic interaction between enzyme and CNC. (auburn.edu)
Applications1
  • Their systems, instruments, and assays are fitted to the needs of life science laboratories that are focused on a range of varying applications. (excedr.com)
Distinct1
  • Using in vitro and cellular techniques, we present a mVP40 assembly model highlighting two distinct oligomerization interfaces: the (N-terminal domain (NTD) and C-terminal domain (CTD)) in mVP40. (uci.edu)
High1
  • Affibody molecules hold great promise as carriers of cytotoxic drugs for cancer therapy due to their typically high affinity, easy production, and inherent control of the drug molecules' loading and spatial arrangement. (mdpi.com)
Separate1
  • Techniques used to separate mixtures of substances based on differences in the relative affinities of the substances for mobile and stationary phases. (lookformedical.com)
Phases1
  • In recent years we have seen a revival of the science aspects of the area driven by advances in the understanding of intermolecular interactions, cooperative phenomena and condensed phases. (lu.se)
Wash1
  • Wash gel thoroughly with buffer before use to remove sugar added to stabilize the lectin. (vectorlabs.com)