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AmidohydrolasesN-Acetylmuramoyl-L-alanine AmidaseHydrolasesPenicillin AmidasePeptidoglycanGlycoside HydrolasesGlutaratesSubstrate SpecificityEscherichia coliAmino Acid SequenceMolecular Sequence DataEpoxide HydrolasesEscherichia coli ProteinsMutationCarboxylic Ester HydrolasesLysosomesKineticsAcid PhosphataseGlucuronidaseHexosaminidasesMannosephosphatesPeptide HydrolasesMucolipidosesThiolester HydrolasesReceptor, IGF Type 2Acetylglucosaminidasealpha-L-FucosidaseHydrolysisXylosidasesCellulaseCellvibriobeta-GlucosidaseGalactosidasesbeta-MannosidaseEndo-1,4-beta XylanasesXylansAcid Anhydride HydrolasesArylsulfatasesbeta-N-AcetylhexosaminidasesDisaccharidasesSequence Homology, Amino AcidPyrophosphatasesCathepsinsGlucosidasesMannosidasesN-Glycosyl HydrolasesCathepsin DCell WallMonocrotophosCloning, MolecularTransferases (Other Substituted Phosphate Groups)CellulasesCatalytic DomainEsterasesVacuolesSequence AlignmentCatalysisCelluloseHydrogen-Ion ConcentrationBacterial ProteinsSaposinsAcetylesteraseCellulose 1,4-beta-CellobiosidaseSulfatasesModels, MolecularSucraseSterol EsterasePhosphoric Monoester HydrolasesGlucansalpha-Mannosidasealpha-GlucosidasesEnzyme StabilityCathepsin AOligosaccharidesCrystallography, X-RayPalmitoyl-CoA HydrolasePhylogenyNeocallimastigalesAlteromonasAspartylglucosylaminaseFibrobacterGlucan Endo-1,3-beta-D-GlucosidasePhosphoric Diester HydrolasesEpoxy CompoundsRecombinant ProteinsSequence Analysis, DNABacteriolysisCarboxylesteraseBinding SitesGlucan 1,4-beta-GlucosidasePiromycesPolysaccharidesChitinaseAspergillus nigerGlycosyltransferasesCarboxypeptidasesUbiquitin ThiolesteraseBase SequenceLeucyl AminopeptidaseEndopeptidases
FAAHPeptidoglycan hydrolasesEnzymesAcidAmideMurein hydrolasesNAAAEnzymeProteinNucleophileAutolysinBacterial cell wallPeptideActivityEndopeptidasesMembraneFunctionalTotalProductMurein hydrolaseEsteraseAliphaticCarbon-nitrogenLipasePeptidoglycanCholoylglycine hydrolaseUrethaneMechanismInterproscanReactionDomainCellFunctions
FAAH7
  • While it was discovered within the last decade, its structural similarity to the more familiar acid ceramidase (AC) and functional similarity to fatty acid amide hydrolase (FAAH) allow it to be studied extensively. (wikipedia.org)
  • While NAAA operates much like fatty acid amide hydrolase (HUGO gene symbol: FAAH), the two enzymes are not homologous. (wikipedia.org)
  • The actions of these lipid messengers are terminated by enzyme-mediated hydrolysis, which is catalyzed by 2 intracellular lipid amidases: N-acylethanolamine acid amidase (NAAA) and fatty acid amide hydrolase (FAAH). (scienzainrete.it)
  • Fatty acid amide hydrolase (FAAH), belonging to an amidase-signature (AS) protein family, is a membrane-bound protein predominantly in microsomal and mitochondrial fractions that catalyzes the hydrolysis of several bioactive lipids, including anandamide, 2-arachidonoylglycerol (2-AG) and oleamide. (apexbt.com)
  • TNFα treatment of DU145 cells increased mRNA levels of PTSG2 (gene of COX-2) and decreased the mRNA of the AEA synthetic enzyme N -acyl-phosphatidylethanolamine selective phospholipase D. mRNA levels of the AEA hydrolytic enzymes fatty acid amide hydrolase (FAAH) and N -acylethanolamine-hydrolyzing acid amidase were not changed. (researcher-app.com)
  • Fatty acid amide hydrolase , (FAAH, Oleamide hydrolase, Anandamide amidohydrolase), is an integral membrane protein that hydrolyzes bioactive amides, including anandamide , to free fatty acid and ethanolamine. (tocris.com)
  • FAAH belongs to the serine hydrolase enzyme family. (tocris.com)
Peptidoglycan hydrolases3
Enzymes7
  • Currently, there is little information about NAAA's tertiary structure, due to its low homology to any of the other enzymes in the choloylglycine hydrolase family for which there is significant 3-dimensional structural information. (wikipedia.org)
  • This gene encodes a fatty acid amide hydrolase that shares a conserved protein motif with the amidase signature family of enzymes. (antikoerper-online.de)
  • These enzymes isolate the sugar backbone from the PGN stem peptide and are members of N -acetylmuramyl-L-alanine amidase. (creative-biolabs.com)
  • These enzymes in S. aureus include N-acetyl muramidase, N-acetyl glucosaminidase, N-acetylmuramyl-l-alanine amidase and endopeptidase (Ramadurai et al. (jnk-receptor.com)
  • OATs are Ntn (N-terminal nucleophile) enzymes, but are distinct from the better-characterized Ntn hydrolase enzymes as they catalyse acetyl transfer rather than a hydrolysis reaction. (ox.ac.uk)
  • The larger domain of the structure consists of an alphabetabetaalpha sandwich as in the structures of Ntn hydrolase enzymes. (ox.ac.uk)
  • However, differences in the connectivity reveal that OATs belong to a structural family different from that of other structurally characterized Ntn enzymes, with one exception: unexpectedly, the alphabetabetaalpha sandwich of ORF6 (where ORF stands for open reading frame) displays the same fold as an DmpA (L-aminopeptidase D-ala-esterase/amidase from Ochrobactrum anthropi), and so the OATs and DmpA form a new structural subfamily of Ntn enzymes. (ox.ac.uk)
Acid10
  • N-acylethanolamine acid amide hydrolase (NAAA) EC 3.5.1. (wikipedia.org)
  • Giang DK, Cravatt BF: Molecular characterization of human and mouse fatty acid amide hydrolases. (t3db.ca)
  • Wan M, Cravatt BF, Ring HZ, Zhang X, Francke U: Conserved chromosomal location and genomic structure of human and mouse fatty-acid amide hydrolase genes and evaluation of clasper as a candidate neurological mutation. (t3db.ca)
  • 1. Discovery of a novel mechanistic approach to control inflammation and pain response: N-Acylethanolamine Acid Amidase (NAAA) inhibition. (scienzainrete.it)
  • 2019 ) Design and synthesis of cyanamides as potent and selective N-acylethanolamine acid amidase inhibitors. (academictree.org)
  • 2019 ) Piperidine and piperazine inhibitors of fatty acid amide hydrolase targeting excitotoxic pathology. (academictree.org)
  • Quantitative Proteome Analysis of Mouse Liver Lysosomes Provides Evidence for Mannose 6-phosphate-independent Targeting Mechanisms of Acid Hydrolases in Mucolipidosis II. (uni-bielefeld.de)
  • N-carbamoyl-L-amino-acid hydrolase. (edu.pl)
  • This caused changes in the transferase/hydrolase ratios, ranging from a 40-fold decrease for alphaF146Y and alphaF146W to a threefold increase for alphaF146L and betaF24A, using 6-aminopenicillanic acid as the nucleophile. (hanze.nl)
  • Tocris offers the following scientific literature for Fatty Acid Amide Hydrolase to showcase our products. (tocris.com)
Amide1
  • non-peptide linear amide C-N hydrolase) inhibitor that interferes with the activity of a glutaminase (EC 3.5.1.2) and thus the generation of glutamate from glutamine. (mcw.edu)
Murein hydrolases1
NAAA1
  • NAAA is an N-terminal nucleophile cysteine amidase that displays a strong preference for saturated FAEs such as PEA. (scienzainrete.it)
Enzyme3
  • Our results indicate that peptidoglycan regulators and adaptors are part of PG biosynthetic multi-enzyme complexes, regulating and potentially coordinating the spatiotemporal action of PG synthases and hydrolases. (uu.nl)
  • The metabolic serine hydrolase family is, arguably, one of the largest functional enzyme classes in mammals, including humans, comprising 1-2% of the total proteome. (inrae.fr)
  • This enzyme family uses a conserved nucleophilic serine residue in the active site to perform diverse hydrolytic reactions and consists of proteases, lipases, esterases, amidases, and transacylases, which are prototypical members of this family. (inrae.fr)
Protein3
  • A Salmonella enterica flagellar protein FlgJ is believed to consist of two functional domains, the N-terminal half acting as a scaffold or cap essential for rod assembly and the C-terminal half acting as a PG hydrolase (PGase) that makes a hole in the PG layer to facilitate rod penetration. (elsevierpure.com)
  • Here, we identify the OM lipoprotein NlpI as a general adaptor protein for PG hydrolases. (uu.nl)
  • It is a bifunctional protein consisting of a propeptide region, an amidase, and an endo- β - N -acetylglucosaminase domain. (creative-biolabs.com)
Nucleophile1
  • is a member of the choloylglycine hydrolase family, a subset of the N-terminal nucleophile hydrolase superfamily. (wikipedia.org)
Autolysin2
Bacterial cell wall1
Peptide2
  • The influence of salts of heavy metals on trypsin-like peptide hydrolase of drosophila larvae partly refined by methods of salting-out, gel chromatography and electrophoresis has been researched. (ukrbiochemjournal.org)
  • Since metal chlorides were used in all cases, it is the differentiated effect of metal ions on manifestations of amidase activity of trypsin-like peptide hydrolase of drosophila larvae, which rather may be considered as proved than the effect of chlorine ions. (ukrbiochemjournal.org)
Activity3
  • Enlargement of the mesh-like sacculus requires the combined activity of peptidoglycan synthases and hydrolases. (uu.nl)
  • Mg 2+ -АТР-hydrolase activity in all cases is much more resistant to studied agents. (ukrbiochemjournal.org)
  • A low amidase activity was also observed for the semisynthetic penicillins amoxicillin and ampicillin and the cephalosporins cefadroxil and cephalexin, for which the kcat values were fivefold to 10-fold lower than the wild-type values. (hanze.nl)
Endopeptidases1
Membrane1
  • Salvage of polymeric PGN presumably requires the removal of peptides from PGN by an unknown amidase, concomitantly with the translocation of the polymer across the outer membrane. (karger.com)
Functional1
  • The specific features of functional lability of the rat colon smooth muscle (CSM) АТР-hydrolases were studied. (ukrbiochemjournal.org)
Total1
  • This graph shows the total number of publications written about "Penicillin Amidase" by people in this website by year, and whether "Penicillin Amidase" was a major or minor topic of these publications. (musc.edu)
Product1
  • The reduced specificity for the product and the high initial transferase/hydrolase ratio of betaF24A resulted in high yields in acyl transfer reactions. (hanze.nl)
Murein hydrolase1
  • One such YSIRK-containing protein is the murein hydrolase LytN. (nih.gov)
Esterase2
  • Screenings based on model molecules were performed leading to the selection of an efficient amidase (E4143) able to hydrolyze the urethane bond of a low molar mass molecule and an esterase (E3576) able to hydrolyze a waterborne polyester polyurethane dispersion. (inra.fr)
  • It was characterized as a novel amidase/esterase, differing in some properties from other known amidases/esterases. (inrae.fr)
Aliphatic4
  • Aliphatic amidases ( EC 3.5.1.4 ), which enable prokaryotes to use acetamides as both carbon and nitrogen source. (embl.de)
  • The family includes: Nitrilase EC:3.5.5.1, Aliphatic amidase EC:3.5.1.4, Biotidinase EC:3.5.1.12, Beta-ureidopropionase EC:3.5.1.6. (umbc.edu)
  • This walkthrough uses a deep mutational scanning dataset of an aliphatic amide hydrolase from Pseudomonas aeruginosa generated by Wrenbeck et al. . (openprotein.ai)
  • This dataset has activity measurements for single substitution mutants of aliphatic amidase from Pseudomonas aeruginosa on three substrates (acetamide, isobutyramide, and propionamide). (openprotein.ai)
Carbon-nitrogen3
  • The carbon-nitrogen hydrolase domain is characterised by several conserved motifs, one of which contains a cysteines that is part of the catalytic site in nitrilases. (embl.de)
  • Carbon-nitrogen hydrolase. (umbc.edu)
  • This family contains hydrolases that break carbon-nitrogen bonds. (umbc.edu)
Lipase1
  • We identified three PLA-degrading genes encoding lipase or hydrolase. (inrae.fr)
Peptidoglycan1
Choloylglycine hydrolase1
  • is a member of the choloylglycine hydrolase family, a subset of the N-terminal nucleophile hydrolase superfamily. (wikipedia.org)
Urethane1
  • The purified enzyme (urethane hydrolase) was found to be homogeneous on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. (inrae.fr)
Mechanism1
  • Mechanism of microsomal epoxide hydrolase. (nih.gov)
Interproscan1
Reaction2
  • Microsomal epoxide hydrolase (MEH) catalyzes the addition of water to epoxides in a two-step reaction involving initial attack of an active site carboxylate on the oxirane to give an ester intermediate followed by hydrolysis of the ester. (nih.gov)
  • The D226N mutant undergoes hydrolytic autoactivation with a half-life of 9.3 days at 37 degreesC, suggesting that the mutant is still capable of catalyzing the hydrolytic half-reaction (in this instance an amidase reaction) and confirming that D226 is in the active site. (nih.gov)
Domain1
  • the E-value for the CN_hydrolase domain shown below is 6.9e-19. (embl.de)
Cell1
  • Amidase Activity of AmiC Controls Cell Separation and Stem Peptide Release and Is Enhanced by NlpD in Neisseria gonorrhoeae. (musc.edu)
Functions1