AnatomyDiseasesChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesDisciplines and OccupationsTechnology, Industry, AgricultureNamed GroupsHealth Care
Corneal TransplantationHydrogelsEye BanksDescemet Stripping Endothelial KeratoplastyFetal Tissue TransplantationTissue DonorsEpikeratophakiaBrain Tissue TransplantationTissue PreservationCorneaHydrogelCorneal DiseasesEndothelium, CornealGraft SurvivalBiocompatible MaterialsPolyethylene GlycolsBlood DonorsGraft RejectionTransplantation, HomologousLiving DonorsTissue EngineeringTransplantation, HeterologousCells, ImmobilizedMechanical PhenomenaElastic ModulusMaterials TestingTissue ScaffoldsPolyhydroxyethyl MethacrylateBiomimetic MaterialsRheologyHyaluronic AcidAlginatesIridoid GlycosidesCross-Linking ReagentsHexuronic AcidsAcrylamidesGlucuronic AcidGelatinMethacrylatesPorosityPolymersPhotochemical ProcessesClick ChemistryNorbornanesPolymerizationCompressive StrengthDelayed-Action Preparations
RegenerationTransplantationEpitheliumInflammationConstructsCollagenWoundTransparencyMicroenvironmentCorneasEndothelial KeratoplastyDefectsDiseasesOrgansRegenerative MedicineMethodsStem cellsBiomedicalBiologicalCompositeCellularFragmentsClinicalCardiacFibersNanoparticlesConventionalVitroFungalSuitableOxygenCellsContact lensMimicHumanNaturalCultureInjuriesSkinModelsImproveHealthyEffectReduceVolumeDelivery
Regeneration1
  • She was awarded with a postdoc fellow from the Ministry of Science at the Technical University of Catalonia in Biomaterials for bone tissue regeneration and since 2010 has been professor at this university. (ibecbarcelona.eu)
Transplantation4
  • Currently, 12.7 million patients are waiting for corneal transplantation, one of the major treatments for severe Glaucoma, while only 1 in 70 of the needs are covered because of the limited corneal donor. (ncsu.edu)
  • Development of a suitable carrier for engineering the CE to address a major clinical requirement of healthy donor tissues for transplantation. (arvojournals.org)
  • Company Description CorneaGen provides corneal endothelial cells for transplantation, as well as related supplies and devices. (ncbiotech.org)
  • 3D bioprinting techniques have brought considerable innovation in biomedicine, especially in the field of tissue engineering, allowing the production of 3D organ and tissue models for in vivo transplantation purposes or for in-depth and precise in vitro analyses. (preprints.org)
Epithelium2
  • [ 42 ] and UV-CXL corneal epithelium intact (epi-on) or removed (epi-off) techniques. (medscape.com)
  • Other recent successes include the approval of using patient-derived limbal stem cells to repair damaged corneal epithelium and using adult stem cells to treat fistulas associated with Crohn's disease. (medicaltrend.org)
Inflammation4
  • Inflammatory diseases are often characterised by excessive neutrophil infiltration from the blood stream to the site of inflammation, which damages healthy tissue and prevents resolution of inflammation. (nature.com)
  • However, excessive or unresolved inflammation can damage healthy tissue and result in chronic inflammatory conditions such as rheumatoid arthritis, ulcerative colitis, cardiomyopathies, cardiovascular disease, and asthma. (nature.com)
  • These events must be tightly regulated to prevent excessive inflammation and tissue damage. (nature.com)
  • The wound healing rate, epithelialization, and formation of granulation tissue were examined, and biomarkers reflecting regulation of proliferation and inflammation were quantified by immunostaining and Western blotting. (bmj.com)
Constructs2
  • Professors Yang Zhang and Jessica M. Gluck are working together to develop a rapid nanoscopic imaging platform to study and validate the nanoscopic functions of biomedical textiles-incorporated tissue-engineered constructs to serve as artificial cornea. (ncsu.edu)
  • μResins can be sculpted within seconds with tomographic light projections into centimetre-scale, granular hydrogel-based, convoluted constructs. (regenhu.com)
Collagen3
  • Ultraviolet corneal collagen cross-linking (UV-CXL) is the only procedure that is believed to slow the progression of keratoconus. (medscape.com)
  • It is used to increase the rigidity of the cornea by inducing additional cross-links within or between collagen fibers using UVA light and a photomediator, riboflavin, with the goal of slowing, possibly stabilizing, and even perhaps reversing, the progression of corneal ectasia in patients with keratoconus. (medscape.com)
  • Riboflavin 5´-phosphate topical ophthalmic (Photrexa, Photrexa Viscous) was approved by the US Food and Drug Administration (FDA) in April 2016 for use in corneal collagen cross-linking (CXL) in combination with the KXL System for the treatment of progressive keratoconus. (medscape.com)
Wound5
  • During this training, she invented several natural biopolymers based in situ forming hydrogels to promote corneal wound healing. (stanford.edu)
  • Objective The present study focused on the development of a poloxamer 407 thermosensitive hydrogel loaded with keratinocyte growth factor-2 (KGF-2) and fibroblast growth factor-21 (FGF-21) as a therapeutic biomaterial in a scald-wound model of type-2 diabetes in Goto-Kakizaki (GK) rats. (bmj.com)
  • The repairing effects of this hydrogel were investigated in a scald-wound model of type-2 diabetes in GK rats. (bmj.com)
  • Conclusions A KGF-2/FGF-21 poloxamer hydrogel accelerated wound healing of scalded skin in GK rats, which was attributed to a synergistic effect of KGF-2-mediated cellular proliferation and FGF-21-mediated inhibition of inflammatory responses. (bmj.com)
  • The keratin is derived from the chicken was the primary material on the hydrogels due to attractively for the potential wound healing application. (preprints.org)
Transparency3
  • In a rabbit model of DMEK surgery, SF artificial endothelial graft restored the corneal transparency and thickness at 6 week of follow-up. (arvojournals.org)
  • Given that nearly a half of all the transplantations performed in a year are for replacing only the dysfunctional endothelium, 2 the monolayer of cells that maintain corneal transparency, research has focussed on engineering this layer in the laboratory. (arvojournals.org)
  • 8 nm).Ppy NPs effectively augment the conductivity, surface roughness, andthickness of BC composites despite reducing scaffolds' transparency.BC-Ppy composites were flexible (up to 10 mM Ppy), maintained theirintricate 3D extracellular matrix-like mesh structure in all Ppy concentrationstested, and displayed electrical conductivities in the range of nativecardiac tissue. (ibecbarcelona.eu)
Microenvironment3
  • The tissue and ECM will provide a natural microenvironment for the cells. (newmediabusinessblog.org)
  • Hydrogel antibacterial agent is an ideal antibacterial material because of it could diffuses antibacterial molecules into the decayed area by providing a suitable microenvironment and the hydrogel acts as a protective barrier on the decay interface. (preprints.org)
  • Abstract In living tissues, cells express their functions following complex signals from their surrounding microenvironment. (regenhu.com)
Corneas2
  • She also established two corneal disease models in rodents and large animals with surgical tool-inner stopper trephines, which she invented to create consistent corneal defects in animal corneas. (stanford.edu)
  • UV-CXL generally does not improve visual acuity (or at most improves 1-2 lines), although UV-CXL improves corneal clarity over untreated keratoconic corneas. (medscape.com)
Endothelial Keratoplasty1
  • Replacing the affected cornea in part (lamellar or endothelial keratoplasty) or total (penetrating keratoplasty) with a healthy donor tissue is the current accepted standard of care. (arvojournals.org)
Defects1
  • Full-thickness skin defects are mainly represented by acute carcinologic excisions, donor-site scarring of reconstructive procedures, burn injuries, and traumatic and chronic wounds. (frontiersin.org)
Diseases4
  • Her ongoing projects are developing nanoparticles (funded by the NIH K99/R00 award) and hydrogels to treat various eye diseases. (stanford.edu)
  • The low endogenous regenerative capacity of the heart,added tothe prevalence of cardiovascular diseases, triggered the advent ofcardiac tissue engineering in the last decades. (ibecbarcelona.eu)
  • Extracellular vesicles (EVs) -- nanometer sized messengers that travel between cells to deliver cues and cargo -- are promising tools for the next generation of therapies for everything from autoimmune and neurodegenerative diseases to cancer and tissue injury. (regenerativemedicine.net)
  • In this study, the Chitosan-Ag (CS-Ag) complex hydrogels were prepared using the physical crosslinking method, which used for controlling postharvest diseases in grape. (preprints.org)
Organs2
  • For instance, cells can naturally migrate, localize, and even proliferate in specific tissues or organs. (medicaltrend.org)
  • In this review, we focus on the bioactive and mechanical characteristics that natural hydrogels may possess to allow efficient production of organs and tissues for biomedical applications, emphasizing the reinforcement techniques to improve their biomechanical properties. (preprints.org)
Regenerative Medicine2
  • The field of regenerative medicine holds the promise of engineering damaged or diseased tissues previously thought to be irreparable. (nih.gov)
  • This meeting featured in-person presentations by leading public and private companies, highlighting technical and clinical achievements over the past 12 months in the areas of cell therapy, gene therapy, gene editing, tissue engineering and broader regenerative medicine technologies. (meetingonthemesa.com)
Methods3
  • Research design and methods In this study, a poloxamer 407 thermosensitive hydrogel loaded with KGF-2 and/or FGF-21 was prepared and its physical and biological properties were characterized. (bmj.com)
  • The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture . (wikipedia.org)
  • Since Haberlandt's original assertions, methods for tissue and cell culture have been realized, leading to significant discoveries in biology and medicine. (wikipedia.org)
Stem cells2
  • The heart was made from the fatty tissue of the patient and was converted into stem cells. (newmediabusinessblog.org)
  • Extracellular vesicles (EVs) and their mimics from mesenchymal stem cells (MSCs) are promising drug carriers to improve cancer treatment, but their application is hindered by donor variations and expansion limitations of conventional tissue-derived MSCs. (biomedcentral.com)
Biomedical2
  • For biomedical 3D printing, the filament is called bio-ink and contains three key components, biological materials, hydrogels and biochemical factors [10] . (newmediabusinessblog.org)
  • The aim of this research study was to develop hydrogels samples for using in potential biomedical applications. (preprints.org)
Biological2
  • The first component of biological material is made of two properties, living cells and tissue/extracellular matrix (ECM). (newmediabusinessblog.org)
  • Natural-derived hydrogels, especially those obtained from the decellularization of biological tissues, are promising bioinks for 3D printing purposes, as they present the best biocompatibility characteristics. (preprints.org)
Composite2
  • This work proposes a microfibers-hydrogel assembled composite as delivery vehicle able to combine into a single system both burst and prolonged release of lactate. (ibecbarcelona.eu)
  • The structure and surface morphology of CS-Ag composite hydrogels were characterized by UV-Vis, FTIR, SEM, and XRD. (preprints.org)
Cellular3
  • Histologic analysis showed that the SF artificial endothelial graft was attached and integrated on the surface of the corneal stroma without a significant inflammatory reaction, and rabbit CECs consisted in a monolayer that showed their characteristic markers ZO-1 and Na + /K + ATPase, suggesting proper intercellular junctions and cellular pump function. (arvojournals.org)
  • Company Description Caeregen Therapeutics develops therapies which repair, restore, and protect damaged and diseased tissues by reactivating the pathways and signaling which accompany normal cellular and tissue development. (ncbiotech.org)
  • A KGF-2/FGF-21 poloxamer hydrogel promoted cellular migration without apoptosis. (bmj.com)
Fragments2
  • In 1913, E. Steinhardt, C. Israeli, and R. A. Lambert grew vaccinia virus in fragments of guinea pig corneal tissue. (wikipedia.org)
  • Part of the innate immune system that is the body s first line defense against infection, dendritic cells sense the presence of pathogens in the blood or other tissues, ingest infecting organisms, and then display fragments of the invaders on their surface, which alerts and activates both killer and helper T cells (CD4 and CD8 cells), essentially teaching them to target the specific pathogen. (regenerativemedicine.net)
Clinical1
  • Her current research is focused on a UGA collaboration to establish a new approach of corneal organoid generation for a new strategy of future clinical therapies, such as aniridia. (nih.gov)
Cardiac4
  • ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, and unlike ZO-1, which is also expressed at cell junctions of cardiac myocytes, ZO-2 is not expressed in nonepithelial tissue. (thermofisher.com)
  • Our results highlight the remarkablepotential use of BC-Ppy scaffolds as a cardiac patch in tissue regenerativetherapies. (ibecbarcelona.eu)
  • Because these vesicles are derived from vascular tissue, which is uniquely tuned to sense hypoxic stress, the researchers hypothesized that the cargo they carry could provide direct protection to cardiac muscle. (regenerativemedicine.net)
  • Our findings indicate that EEVs could protect cardiac tissue from reoxygenation injury in part by supplementing the injured cells with proteins and signaling molecules that support different metabolic processes, paving the way for new therapeutic approaches," said Andr G. Kl ber, a Visiting Professor of Pathology at Harvard Medical School and co-author of the study. (regenerativemedicine.net)
Fibers1
  • Incorporating hydrogels into knit and woven technologies as fibers would open up a large design space of advanced textiles. (ncsu.edu)
Nanoparticles1
Conventional1
  • Interstitial microvoids enhanced differentiation of multiple stem/progenitor cells (vascular, mesenchymal, neural), otherwise not possible with conventional bulk hydrogels. (regenhu.com)
Vitro1
  • The inhibitory effects of CS-Ag hydrogel on disease in grape caused by P. expansum, A. niger and B. cinerea were investigated both in vivo and in vitro. (preprints.org)
Fungal2
  • In practice, the term "cell culture" now refers to the culturing of cells derived from multicellular eukaryotes , especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture , fungal culture, and microbiological culture (of microbes ). (wikipedia.org)
  • This revealed CS-Ag hydrogels plays a critical role in controlling fungal disease in grape. (preprints.org)
Suitable1
  • The purpose of this study was to determine if non-mulberry varieties of silk are suitable for the culture of corneal endothelium (CE). (arvojournals.org)
Oxygen3
  • Company Description KlearOptix develops materials for high oxygen permeable silicone hydrogel, rigid gas permeable (RGP), and hybrids for contact lens applications. (ncbiotech.org)
  • So-called ischemia-reperfusion injury (IRI) or reoxygenation injury, happens when blood supply returns to tissue after a period of lack of oxygen. (regenerativemedicine.net)
  • The researchers induced injury by three hours of oxygen restrictions followed by 90 minutes of reoxygenation and then measured the fraction of dead cells and the contractile force of the tissue. (regenerativemedicine.net)
Cells10
  • Human and rabbit artificial corneal endothelial grafts were developed through the culture of human and rabbit corneal endothelial cells (CECs) on SF films. (arvojournals.org)
  • Cell culture or tissue culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. (wikipedia.org)
  • After the cells of interest have been isolated from living tissue , they can subsequently be maintained under carefully controlled conditions. (wikipedia.org)
  • Tissue culture commonly refers to the culture of animal cells and tissues, with the more specific term plant tissue culture being used for plants. (wikipedia.org)
  • The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century. (wikipedia.org)
  • [12] He suggested that the potentialities of individual cells via tissue culture as well as that the reciprocal influences of tissues on one another could be determined by this method. (wikipedia.org)
  • The 4DMT customized and evolved vectors were invented for low dose delivery via clinically routine, well-tolerated, and minimally invasive routes of administration, and efficient transduction of cells in target tissues, with reduced immunogenicity and resistance to pre-existing antibodies. (meetingonthemesa.com)
  • The heart tissue treated with EEVs had half as many dead cells and had a contractile force four times higher than the untreated tissue after injury. (regenerativemedicine.net)
  • One strategy to increase cure rates is the identification of patient-specific drug responses in tissue models that mimic the interaction between patient cancer cells and tumor environment. (regenhu.com)
  • Bioprinted channels are coated with endothelial cells post printing to form a dense vessel - tissue barrier. (regenhu.com)
Contact lens1
  • When rigid contact lenses are no longer tolerated, some patients can maintain contact lens wear and usable visions with hydrogel contact lenses, piggyback contact lenses, or scleral contact lenses. (medscape.com)
Mimic1
  • Organ-on-chip platforms mimic the structure and function of native tissue and allow researchers to observe, in real time, the effects of injuries and treatments in human tissue. (regenerativemedicine.net)
Human4
  • It is an FDA-registered human tissue establishment. (ncbiotech.org)
  • The researchers demonstrated this functionality in human tissue using a heart-on-a-chip with embedded sensors that continuously tracked the contractions of the tissue. (regenerativemedicine.net)
  • The team tested the effect of EEVs on human heart tissue using the heart-on-a-chip model developed by the Disease Biophysics Group at SEAS. (regenerativemedicine.net)
  • The tissue model thereby mimics structure and function of human soft tissue with endothelial cell-coated larger vessels for perfusion and micro-vessel networks within the hydrogel-matrix. (regenhu.com)
Natural1
  • Despite this, many natural hydrogels do not possess the necessary mechanical properties to allow a simple and immediate application in the 3D printing process. (preprints.org)
Culture4
  • In order to reduce morbidity associated with STSG harvesting, researchers have developed autologous dermo-epidermal substitutes (DESs) using cell culture, tissue engineering, and, more recently, bioprinting approaches. (frontiersin.org)
  • The term "tissue culture" was coined by American pathologist Montrose Thomas Burrows . (wikipedia.org)
  • [6] In 1885 Wilhelm Roux removed a section of the medullary plate of an embryonic chicken and maintained it in a warm saline solution for several days, establishing the basic principle of tissue culture. (wikipedia.org)
  • Gottlieb Haberlandt first pointed out the possibilities of the culture of isolated tissues, plant tissue culture . (wikipedia.org)
Injuries1
  • In the case of severe burn injuries, it remains the only surgical solution to ensure patient survival, and surgeons need to manage with a donor-site shortage. (frontiersin.org)
Skin3
  • In this review paper, we main-ly focus on the appli-ca-tion of tilapia col-la-gen in the skin, bone/​dentin, neur-al and corneal tis-sue engineering. (jurnalhalal.org)
  • However, this technique has two major drawbacks: the use of general anesthesia for skin harvesting and scar sequelae on the donor site. (frontiersin.org)
  • A KGF-2/FGF-21 hydrogel accelerated the healing of scalded skin in Goto-Kakizaki rats better than that of a KGF-2 or FGF-21 hydrogel alone. (bmj.com)
Models1
  • The hydrogel samples were made by using the freeze‑thawing method, and they were examined using the Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), porosity amusement, swelling ratio and keratin release kinetic models that analyzed using (zero-order, first-order Higuchi and Korsmeyer-Peppas models). (preprints.org)
Improve1
  • [ 46 ] to improve the corneal integrity prior to surgery on keratoconic eyes. (medscape.com)
Healthy2
  • Anterior segment optical coherence tomography revealed the SF graft as a fully integrated component in the corneal tissue, displaying a similar corneal thickness and endothelial cell count when compared with its healthy contralateral cornea. (arvojournals.org)
  • However, the huge gap in the demand and supply of healthy donor tissues is a well acknowledged bottleneck for timely transplantations in many countries. (arvojournals.org)
Effect1
Reduce1
  • Archerfish develops small molecule drugs to reduce tissue fibrosis and corneal scarring. (ncbiotech.org)
Volume1
  • Hydrogels have unique responsive volume change and ionic conductivity capabilities that have led to their use for biomolecular separations, ionic circuitry, and stimuli-responsive actuators. (ncsu.edu)
Delivery1
  • It also binds and activates neuropilin-1, leading to a temporary opening of the tumor and an enhanced delivery of anti-cancer agents into the tumor tissue. (wikipedia.org)