AnatomyOrganismsDiseasesChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesDisciplines and OccupationsInformation ScienceHealth Care
In Situ HybridizationIn Situ Hybridization, FluorescenceNucleic Acid HybridizationRNA, MessengerHybridization, GeneticMolecular Sequence DataDNA ProbesBase SequenceRNA ProbesPolymerase Chain ReactionComparative Genomic HybridizationCloning, MolecularImmunohistochemistryOligonucleotide ProbesDigoxigeninChromosome AberrationsBlotting, NorthernChromosome MappingAmino Acid SequenceGene ExpressionDNA, BacterialGene AmplificationSequence Analysis, DNADNA, ComplementaryKaryotypingPhylogenyBlotting, SouthernRNA, Ribosomal, 16SDNADNA, RibosomalGene Expression Regulation, DevelopmentalChromosome BandingOligonucleotide Array Sequence AnalysisTranslocation, GeneticAneuploidyReverse Transcriptase Polymerase Chain ReactionGene DosageSpecies SpecificityTissue DistributionDNA, ViralTranscription, GeneticDNA PrimersSequence Homology, Amino AcidGene LibraryCarcinoma in SituGene Expression ProfilingRNASequence Homology, Nucleic AcidNucleic Acid ProbesChromosomes, Human, Pair 1GenesGene Expression RegulationChromosomes, Human, Pair 17Chromosome DeletionChromosomes, Human, Pair 7Paraffin EmbeddingPeptide Nucleic AcidsOrgan SpecificityPrimed In Situ LabelingCytogenetic AnalysisSensitivity and SpecificityRNA, BacterialChromosomes, Human, Pair 11Restriction MappingMolecular Probe TechniquesPhenotypeChromosomes, HumanY ChromosomeChromosomes, Human, Pair 8RNA, ViralChromosomes, Human, Pair 12Genes, erbB-2CytogeneticsAutoradiographyChromosomes, Artificial, BacterialBrainSequence AlignmentInterphaseChromosome PaintingGene RearrangementChromosomes, Human, Pair 14Chromosome DisordersDNA, NeoplasmEmbryonic and Fetal DevelopmentChromosomes, Human, Pair 18DNA Restriction EnzymesChromosomesRats, Sprague-DawleyGenes, BacterialChromosomes, Human, Pair 3Chromosomes, Human, Pair 13Transcription FactorsImmunoenzyme TechniquesGenetic MarkersPlasmidsTissue FixationGenes, rRNACell LineMetaphaseCells, Cultured
Fluorescence in situ hybriComparative genomic hybriProbesChromosomeFISH
Fluorescence in situ hybri2
Comparative genomic hybri2
  • The characterisation of the lymphoma cell line U937, using comparative genomic hybridisation and multi-plex FISH. (wikidata.org)
  • More recently, the technique of comparative genomic hybridisation (CGH) using metaphase chromosomes has been applied to this clinical problem. (bmj.com)
Probes4
  • The continuous flow of probes and shaping liquid on these selected cells resulted in a 120-fold reduction of the hybridization time compared with the standard protocol (3 min vs. 6 h) and efficient rinsing, thereby shortening the total FISH assay time for centromeric probes. (springer.com)
  • It relies on the sequence-specific hybridization of probes to their complementary targets in individual cells, followed by direct or indirect detection of the labelled probe. (springer.com)
  • In patients with normal karyotypes, the clinical features may still suggest appropriate regions to be screened for microscopic imbalance by fluorescent in situ hybridisation (FISH) analysis using locus specific probes. (bmj.com)
  • FISH probes designed to detect subtelomeric rearrangements have given a significant diagnostic yield of around 6% in patients with unexplained learning disability and dysmorphic features, 1- 3 but interstitial chromosomal deletions and duplications are not detectable using this method. (bmj.com)
Chromosome1
  • Routine karyotype analysis is not sensitive enough to detect subtle chromosome rearrangements (less than 5 Mb). (bmj.com)
FISH2
  • Diagnosis is made by confirming a deletion in the WHS critical region (WHSCR) using conventional chromosome analysis, fluorescence in situ hybridization (FISH), or chromosomal microarray. (medscape.com)
  • These analyses employ standard cytogenetic and fluorescence in situ hybridisation (FISH), array-based profiling, and flow cytometric isolation and massively parallel sequencing of WDLPS NCs, complemented by transcriptome sequencing. (sarcomahelp.org)