• We examined the osmotic regulation of ER-β mRNA expression in MNCs using quantitative in situ hybridization histochemistry. (jneurosci.org)
  • Chromogenic in situ hybridization (CISH) is a cytogenetic technique that combines the chromogenic signal detection method of immunohistochemistry (IHC) techniques with in situ hybridization. (wikipedia.org)
  • It was developed around the year 2000 as an alternative to fluorescence in situ hybridization (FISH) for detection of HER-2/neu oncogene amplification. (wikipedia.org)
  • CISH is similar to FISH in that they are both in situ hybridization techniques used to detect the presence or absence of specific regions of DNA. (wikipedia.org)
  • In situ hybridization histochemistry and the study of gene expression in the human brain. (ox.ac.uk)
  • in situ hybridization histochemistry was carried out to localize the gene transcripts. (nih.gov)
  • Three hours after LPS treatment, IL-1 beta and TNF genes were found by in situ hybridization histochemistry to be expressed by "histiocyte-like" cells in the stroma of the ICB. (nih.gov)
  • A short introduction how cytogenetics and molecular cytogenetics were established is followed by technical aspects of fluorescence in situ hybridization (FISH). (frontiersin.org)
  • This review is about "molecular cytogenetics" including 1) the historical perspective of its development from cytogenetics, 2) technical aspects, 3) available probe sets, and 4) variants and applications of the basic fluorescence in situ hybridization (FISH) approach. (frontiersin.org)
  • Hybridizer is a hands-free denaturation and hybridization instrument designed for slide-based fluorescence and chromogenic in situ hybridization (FISH and CISH, respectively) on histology and cytology samples. (dssimage.com)
  • Abbott Molecular ThermoBrite® System offers an easy, safe, system for in-situ hybridization procedures. (dssimage.com)
  • What is Fluorescence In Situ Hybridization (FISH), and how does it work? (dssimage.com)
  • Simultaneous Visualization of Vasopressin and Oxytocin mRNA- Containing Neurons in the Hypothalamus Using Non-Radioactive in situ Hybridization Histochemistry. (neurotree.org)
  • In situ hybridization histochemistry showing the distribution of TRH precursor mRNA in the brain and pituitary of Xenopus laevis. (xenbase.org)
  • Materials and Methods: To investigate both the presence of numerical abnormalities of chromosome 9 and p16 gene alterations in ovarian cancer, we studied 28 cases by the fluorescence in situ hybridization (FISH) technique using a DNA p16 probe and an a-satellite probe specific for chromosome 9. (iiarjournals.org)
  • The aim of the present study was to determine the significance of this tumor suppressor gene for ovarian tumorigenesis, investigating both numerical aberrations of chromosome 9 and p16 gene alterations in 28 cases of ovarian tumors, by the fluorescence in situ hybridization (FISH) technique. (iiarjournals.org)
  • Other advanced techniques include in situ hybridization to identify specific DNA or RNA molecules, and confocal microscopy. (fact-archive.com)
  • The effect of L-3,4-dihydroxyphenylalanine (L-DOPA) on dopamine receptor gene expression in the brain of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-lesioned monkeys was investigated using in situ hybridization histochemistry with measures of changes in relative absorbance. (aspetjournals.org)
  • In situ hybridization histochemistry reveals the alpha 6 mRNA to be located within the granule cell layer of the human cerebellar cortex. (drugbank.com)
  • Streptavidin is broadly used in various applications such as immunoassays, histochemistry, FISH (Fluorescence In Situ Hybridization), flow cytometry, microarrays and blot analysis. (mobitec.com)
  • Using immunocytochemistry and in situ hybridization histochemistry we have identified distinct neuronal, partly chemically coded MTN subpopulations - large glutamatergic and smaller GABAergic neurons. (lazarov.pro)
  • subunit mRNAs in various regions of bovine brain has been investigated using in situ hybridization. (worktribe.com)
  • In addition, we performed in situ hybridization for Apis NOS mRNA and NADPH-diaphorase histochemistry in the honeybee brain. (elsevierpure.com)
  • Localization of collagenase mRNA in rheumatoid arthritis synovium by in situ hybridization histochemistry. (mesotheliomadr.com)
  • Distribution of striatonigral and striatopallidal peptidergic neurons in both patch and matrix compartments: an in situ hybridization histochemistry and fluorescent retrograde tracing study. (wikidata.org)
  • 63 different brain regions in about 10 × 20 × 20 mm size ready for cryostat sectioning and in situ hybridization histochemistry. (msdiscovery.org)
  • Synaptophysin gene expression in human brain: a quantitative in situ hybridization and immunocytochemical study. (ox.ac.uk)
  • We have studied synaptophysin messenger RNA in the neocortex, hippocampus and cerebellum using in situ hybridization histochemistry to see if the encoding transcript can be detected in post mortem human brain and to investigate factors which might influence its abundance. (ox.ac.uk)
  • In situ hybridization histochemistry indicated that these increases in gadd45 mRNA occurred in pyramidal neurons located on the edge of the infarcted cortex. (ox.ac.uk)
  • We detected tau mRNA using in situ hybridization histochemistry in the hippocampus, visual cortex, and cerebellum, and compared its level in Alzheimer's disease with controls. (ox.ac.uk)
  • The CNS nicotinic cholinergic system is studied using techniques that include quantitative receptor autoradiography, quantitative in situ hybridization histochemistry, ion flux assays and behavioral analyses. (uky.edu)
  • Darkfield photomicrographs illustrating the localization of TRH precursor mRNA hybridization signal in selected regions of the brain of white-adapted Xenopus laevis. (xenbase.org)
  • A significant and gene-specific increase in tau mRNA hybridization was found in hippocampal fields CA4 and CA3, with a similar trend in the dentate gyrus. (ox.ac.uk)
  • They are complementary to the targeted sequence and bind to it after a denaturation and hybridization step. (wikipedia.org)
  • DSS offers a complete solution for FISH from reagents, probes, hybridization chambers to FL microscopes with accurate filters and software. (dssimage.com)
  • HyperChrome is the perfect Instrument for denaturation & hybridization procedures during FISH experiments. (dssimage.com)
  • These factors include the presence of solvent (formamide), hybridization temperature, length of hybridization, volume of hybridization solution, degree and method of agitation, use of blocking reagents, concentration and specific activity of the probe, use of molecular agents to increase the rate of nucleic acid reassociation, and the degree of stringency used during the washing of the membrane. (aidstar-one.com)
  • The precision temperature control and sealed chamber offer the ideal environment for controlled IHC and InSitu Hybridization reactions. (southeastpathology.com)
  • The Boekel Scientific programmable slide hybridization system offers researchers a compact 12 position Slide Hybridization system capable of performing complex IHC and InSitu Hybridization reactions. (southeastpathology.com)
  • In order to decrease any non-specific hybridization of the probe to a substrate, blocking agents must be used. (aidstar-one.com)
  • B: In the anterior preoptic area (Poa), a strong hybridization signal was visualized in cells lining the ventricle . (xenbase.org)
  • The Boekel Scientific Programmable Slide Hybridization system offers greater functionality with a compact footprint that compliments our complete line of Hybridization and Slide Hybridization systems. (southeastpathology.com)
  • The tissue samples must then be washed and heated several times to remove any paraffin before the hybridization step. (wikipedia.org)
  • Main markers in immuno-histochemistry (data-sheet reading, optimal antibody new dilution, etc. (unige.it)
  • The sections are especially suitable for studies of tissue and organ development, expression of DNA and RNA by in situ hybridization, and the localization of gene products by immuno-histochemistry. (fdneurotech.com)
  • What is an immuno-histochemistry test? (ballyabio.com)
  • We used a variety of techniques, including anatomical (hybridization histochemistry and receptor autoradiography), molecular biological, transgenic animals, optogenetics, and electrophysiology to explore behavior in the mouse. (nih.gov)
  • In situ hybridization histochemistry and autoradiography were used to examine the cellular distribution of mGluR1 receptor subunit mRNA in the Crus II region and quantitative analysis of mGluR1 expression was obtained by measuring silver grain density in 120 Purkinje cell somata for each case from n=9 control and autism cases (Crus II) and n=8 control and autism cases (vermis). (confex.com)
  • In situ nucleic acid hybridization (chromogenic or multiplex fluorescent ISH, e.g. (northwestern.edu)
  • Suitable for an array of laboratory applications including resuspension, protein binding, nucleic acid purification, histochemistry, hybridization etc, the mixers include interchangeable tube holders for accommodating all common tube sizes from 0.5ml to 50ml. (chemglass.com)
  • Messenger RNA encoding the D2 dopaminergic receptor detected by in situ hybridization histochemistry in rat brain. (ox.ac.uk)
  • The effect of L-3,4-dihydroxyphenylalanine (L-DOPA) on dopamine receptor gene expression in the brain of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-lesioned monkeys was investigated using in situ hybridization histochemistry with measures of changes in relative absorbance. (aspetjournals.org)
  • Because the complex of antigen and antibody is colorless, it is necessary to show the binding site of antigen and antibody by means of histochemistry to achieve qualitative, localized or quantitative research on unknown antigens in tissues or cells. (ballyabio.com)
  • If instead your method is histochemistry then choose a biotinylated secondary antibody. (uk.com)
  • We conclude that autoclaving renders formalin-fixed, paraffin wax-embedded sections of human brain suitable for quantitative in situ hybridization histochemistry. (ox.ac.uk)
  • It cleverly combines the specificity of the immune response and the visibility of histochemistry. (ballyabio.com)
  • . In situ hybridization (ISH) is a quite common technique among developmental research labs, yet its uses are quite diverse and include medical and prenatal diagnostics [ 9 - 16 ]. (hindawi.com)
  • To overcome permeability issues and allow QD conjugate penetration, embryos were treated with proteinase K. The use of QDs dramatically increased sensitivity of whole-mount in situ hybridization (WISH) in comparison with organic fluorophores and enabled fluorescent detection of specific transcripts within cells without the use of enzymatic amplification. (hindawi.com)
  • Most in situ hybridization histochemistry studies of messenger RNA in human brain have been carried out on frozen tissue. (ox.ac.uk)
  • They are complementary to the targeted sequence and bind to it after a denaturation and hybridization step. (wikipedia.org)
  • The tissue samples must then be washed and heated several times to remove any paraffin before the hybridization step. (wikipedia.org)
  • Kohzo is looking for the new members of rel family and will try to in situ hybridization and histochemistry as a first step. (nih.gov)
  • The hybridization signal correlated strongly with that seen in the frozen sections. (ox.ac.uk)