AnatomyOrganismsDiseasesChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesDisciplines and OccupationsAnthropology, Education, Sociology and Social PhenomenaInformation ScienceHealth Care
Nucleic Acid HybridizationIn Situ HybridizationIn Situ Hybridization, FluorescenceDNA ProbesHybridization, GeneticOligonucleotide ProbesMolecular ProbesBase SequenceMolecular Sequence DataRNA ProbesRNA, MessengerMolecular Probe TechniquesComparative Genomic HybridizationCloning, MolecularPolymerase Chain ReactionDNA, BacterialNucleic Acid ProbesDNABlotting, SouthernAmino Acid SequenceSequence Analysis, DNAOligonucleotide Array Sequence AnalysisRNA, Ribosomal, 16SChromosome MappingDNA, RibosomalPhylogenyBlotting, NorthernChromosome AberrationsSpecies SpecificityFluorescent DyesDNA, ComplementaryDigoxigeninGene ExpressionSequence Homology, Nucleic AcidGene AmplificationKaryotypingGenesImmunohistochemistrySensitivity and SpecificityDNA, ViralTranscription, GeneticRNA, BacterialDNA PrimersRestriction MappingGenes, BacterialDNA Restriction EnzymesGene LibraryGene DosageRNAGene Expression ProfilingChromosome BandingPeptide Nucleic AcidsPlasmidsAneuploidyReverse Transcriptase Polymerase Chain ReactionTranslocation, GeneticSequence Homology, Amino AcidChromosome DeletionPhenotypeGene Expression Regulation, DevelopmentalBase CompositionEscherichia coliOligonucleotidesTissue DistributionGene Expression RegulationBacterial Typing TechniquesPolymorphism, Restriction Fragment LengthRNA, ViralRNA, RibosomalCell LineGenes, rRNARepetitive Sequences, Nucleic AcidChromosomes, Human, Pair 1MutationNucleic Acid DenaturationSequence AlignmentBiotinDNA, NeoplasmChromosomes, HumanY ChromosomeReproducibility of ResultsHybrid CellsGenetic MarkersCosmidsParaffin EmbeddingElectrophoresis, Agar GelGenetic VariationChromosomes, Human, Pair 17KineticsChromosomes, Human, Pair 7Organ SpecificityCytogenetic AnalysisGenotypeMultigene FamilyChromosome PaintingDNA, RecombinantPoly AChromosomes, Human, Pair 11AutoradiographyChromosomes, Artificial, Bacterial
SituFluorescenceAssayDetectionRRNAGenomicFluorescent probesSpecificityChromosomeDetectSequencesChromosomesOligonucleotide probesMolecularTissueImmunohistochemistryCytogeneticRadioactiveNucleic acidsGenomeSNPsStem-loop probeDigoxigeninMicroarraysDiagnosticsSensitivityLife SciencesVivoDemonstrateSilicoAssaysSequenceGeneProtocolsMoleculesMultiplexSpecificMethodsLocusBacterialConventionalAnalysisProductsPreviously
Situ43
Fluorescence1
  • Advances in fluorescence microscopy, fluorescent dyes, biotechnology, bioinformatics, and research on the human genome project in the late 90's accelerated the development of methods to synthesize and design NA probes for FISH. (springer.com)
Assay11
  • 1,2 A novel probe hybridization quantitative polymerase chain reaction (qPCR) assay targeting the cytochrome oxidase I (COI) gene of P. andersoni was developed. (vin.com)
  • Beyond the bulk fluid assay and droplet devices, use of dsPNA probes may be advantageous in a wide variety of assays that employ homogenous nucleic acid hybridization. (psu.edu)
  • When tested in a FISH assay, Staaur and a new probe, KT18, were found to have the same performance. (edu.au)
  • The continuous flow of probes and shaping liquid on these selected cells resulted in a 120-fold reduction of the hybridization time compared with the standard protocol (3 min vs. 6 h) and efficient rinsing, thereby shortening the total FISH assay time for centromeric probes. (springer.com)
  • The Molecular Inversion Probe assay (Parallele Biosciences, South San Francisco, Calif, USA) [ 6 ] uses probes that can be circularized and contain two sequences complementary to regions adjacent to SNPs in the DNA target in the first step of the genotyping reaction. (hindawi.com)
  • A tissue blot DNA hybridization assay was developed to detect Clavibacter xyli subsp. (usda.gov)
  • The assay used a 560 bp PCR product as the probe which was amplified from the intergenic transcribed spacer region of the 16S/23S ribosomal DNA of Cxx with two universal primers. (usda.gov)
  • Assays on 346 duplicate sets of sugarcane tissue blots indicated that the tissue blot DNA hybridization assay detected Cxx as effectively as a tissue blot immunobinding assay. (usda.gov)
  • The tissue blot DNA hybridization assay can be immediately applicable in many sugarcane diagnostic laboratories to compliment current diagnostic methods, thereby enhancing the sensitivity and accuracy of Cxx detection. (usda.gov)
  • RNAscope ISH Probe High Risk HPV is used in an RNAscope ISH assay for the qualitative detection of HPV E6/E7 mRNA in FFPE tissue specimens. (biospace.com)
  • Loss of detectable HBV DNA by a solution phase hybridization assay is an earlier indicator of response to antiviral therapy than loss of HBeAg. (medscape.com)
Detection13
  • Detection of sequences with moderate or high similarity depends on how stringent the hybridization conditions were applied-high stringency, such as high hybridization temperature and low salt in hybridization buffers, permits only hybridization between nucleic acid sequences that are highly similar, whereas low stringency, such as lower temperature and high salt, allows hybridization when the sequences are less similar. (wikipedia.org)
  • In forensic science, hybridization probes are used, for example, for detection of short tandem repeats (microsatellite) regions and in restriction fragment length polymorphism (RFLP) methods, all of which are widely used as part of DNA profiling analysis. (wikipedia.org)
  • We report a reagentless and reusable electrochemical DNA sensor that exploits competitive binding and target hybridization-induced change in the signalling probe conformation for robust detection of a target DNA sequence. (rsc.org)
  • Nucleic acid hybridization is at the core of many amplification-free molecular diagnostics and detection probe configuration is key to diagnostic performance. (psu.edu)
  • Modified nucleic acids such as peptide nucleic acid (PNA) offer advantages compared to conventional DNA probes allowing for faster hybridization, better stability and minimal sample preparation for direct detection of pathogens. (psu.edu)
  • Here, we compared the sensitivity and specificity of double stranded PNA probes (dsPNA) and PNA molecular beacons targeting E. coli and P. aeruginosa for direct detection of bacterial pathogens. (psu.edu)
  • We further designed and tested an expanded panel of dsPNA probes for detection of a wide variety of pathogenic bacteria including probes for universal detection of eubacteria, Enterobacteriaceae family, and P. mirablis. (psu.edu)
  • It relies on the sequence-specific hybridization of probes to their complementary targets in individual cells, followed by direct or indirect detection of the labelled probe. (springer.com)
  • After hybridization with perfectly complementary labeled targets, the stems of probes are opened for the subsequent detection of these targets. (hindawi.com)
  • We are excited to launch the new CE-IVD RNAscope ISH Probe High Risk HPV, which provides pathologists a highly accurate tool for the direct detection of HPV mRNA to inform treatment selection in oropharyngeal squamous cell carcinoma patients. (biospace.com)
  • RNAscope ISH Probe High Risk HPV is for use in clinical laboratories with the CE-IVD marked BOND RNAscope Brown Detection kit on the automated Leica Biosystems BOND-III stainer. (biospace.com)
  • A variety of detection formats are available, including singleplex detection with SYBR Green or sequence-specific probes or multiplex detection with sequence-specific probes (with or without ROX passive reference dye). (qiagen.com)
  • The FastLane Cell Probe Kit delivers sensitive probe-based detection in real-time one-step RT-PCR without the need for RNA purification. (qiagen.com)
RRNA3
  • rRNA probes have enabled scientists to visualize microorganisms, yet to be cultured in laboratory settings, by retrieval of rRNA sequences directly from the environment. (wikipedia.org)
  • The global standard library of rRNA sequences is constantly becoming larger and continuously being updated, and thus the possibility of a random hybridization event between a specifically-designed probe (based on complete and current data from a range of test organisms) and an undesired/unknown target organism cannot be easily dismissed. (wikipedia.org)
  • ISH was first demonstrated by Gall and Pardue ( 1969 ) using radioactive rRNA probes to visualize extrachromosomal rDNA. (springer.com)
Genomic2
  • Previously, a probe-selection method based on hybridisation of genomic DNA (gDNA) was developed, which enables GeneChips to be used for species that they were not designed for. (cambridge.org)
  • Array comparative genomic hybridization (aCGH) is a type of microarray now routinely used to identify deleted or duplicated regions of DNA sequence in specific chromosomes on a genome-wide basis. (msdmanuals.com)
Fluorescent probes1
  • FISH is a molecular technique that uses fluorescent probes that bind to specific parts of the chromosome which is used to detect and localize the presence or absence of specific DNA sequences. (dssimage.com)
Specificity2
  • Specificity of the product was confirmed with hybridization to a labeled probe. (cdc.gov)
  • The specificity of the product was confirmed by hybridization to a digoxigenin-labeled probe according to the method described in Boehringer Mannhein's Genius System User's Guide for Filter Hybridization. (cdc.gov)
Chromosome6
  • Locus specific, Alphoid/centromeric repeat and whole chromosome probes are some of the types of FISH probes that are being commonly used. (researchandmarkets.com)
  • myTags Custom probes from Daicel Arbor Biosciences have been used successfully in (F)ISH experiments with a wide range of organisms such as animals, plants, and microbes - and for a variety of research applications such as 3D DNA-FISH, RNA-FISH, chromosome painting and barcoding, and much more. (arborbiosci.com)
  • Flexible design -We can design your probes from any genome reference sequence(s), and can accommodate any cytogenetics use case, such as chromosome painting or barcoding, RNA-FISH, and much more. (arborbiosci.com)
  • Both panels include myTags human chromosome 17 centromere probes [ATTO 647N] and chromosomes [stained with Hoescht 33342] pseudo-colored gray. (arborbiosci.com)
  • myTags (F)ISH probes have been used successfully across a diverse range of cytogenetic applications including 3D DNA-FISH, Cryo-FISH, RNA-FISH, Oligopaints, and chromosome painting for a variety of cell types. (arborbiosci.com)
  • Three chromosome-specific repetitive probes labeled with either amino acetyl fluorene (AAF), mercury, or biotin were hybridized simultaneously to metaphase chromosomes prepared from human blood lymphocytes or to interphase tumor nuclei. (nih.gov)
Detect4
  • HP can be used to detect the presence of nucleotide sequences in analyzed RNA or DNA that are complementary to the sequence in the probe. (wikipedia.org)
  • To detect hybridization of the probe to its target sequence, the probe is tagged (or "labeled") with a molecular marker of either radioactive or (more recently) fluorescent molecules. (wikipedia.org)
  • FISH uses fluorescently labeled DNA probes to detect or confirm the presence of genes or chromosomal abnormalities. (marketsandmarkets.com)
  • Gene probes can detect a number of disorders before and after birth. (msdmanuals.com)
Sequences5
  • DNA sequences or RNA transcripts that have moderate to high sequence similarity to the probe are then detected by visualizing the hybridized probe via autoradiography or other imaging techniques. (wikipedia.org)
  • Molecular DNA- or RNA-based probes are routinely used in screening gene libraries, detecting nucleotide sequences with blotting methods, and in other gene technologies, such as nucleic acid and tissue microarrays. (wikipedia.org)
  • The discriminative power of the probe set was further tested in silico on 15 additional complete enterobacterial genome sequences. (biomedcentral.com)
  • Does DSS ImageTech offer a wide range of FISH probes for different target sequences? (dssimage.com)
  • There were two different mismatches in the probe target sites of the HA gene sequences of all isolates ( n = 23) with additional mismatches only at position 7 (template binding site) identified for all eight negative real-time RT-PCR isolates. (who.int)
Chromosomes6
  • FISH Probe, a sophisticated cytogenetic technique, plays a crucial role in identifying severe genetic diseases, monitoring therapeutic drugs, and tracking the abnormalities in chromosomes. (researchandmarkets.com)
  • The probe containing a 120-bp repetitive DNA sequence from rye, hybridized to the entire length of all rye chromosomes, but only to a few sites in 14 wheat chromosomes. (illinois.edu)
  • The overall distribution of this DNA probe in the rye chromosomes has not been detected previously with the use of radioactively labeled probes. (illinois.edu)
  • As a result of the formation of a brown precipitate over sites of hybridization in this technique, the rye chromosomes were entirely brown in color, whereas the wheat chromosomes appeared blue. (illinois.edu)
  • Currently, a range of probes can be synthesized to locate and quantify specific short RNAs, genes, entire chromosomes, and even cells (Evanko 2007 ). (springer.com)
  • DesPlaines, Ill), which includes probes for the centromeres for chromosomes 3, 7, and 17 and has a locus-specific probe for 9p21, was developed to screen for recurrent urothelial carcinoma and was approved by the US Food and Drug Administration (FDA) in 2005. (medscape.com)
Oligonucleotide probes1
Molecular3
  • In molecular biology, a hybridization probe (HP) is a fragment of DNA or RNA of usually 15-10000 nucleotide long which can be radioactively or fluorescently labeled. (wikipedia.org)
  • The primer and probe sets in the HA gene were checked for mismatches, and phylogenetic analyses were performed to determine the molecular epidemiology of these viruses. (who.int)
  • However, mutations performed using both standard molecular methods (con- in the viral genome that generate novel variants cause the ventional RT-PCR and real-time RT-PCR), employing sensitivity of these molecular tests to decrease and may specific primer and probe sets targeted to the matrix, hae- lead to false-negative results. (who.int)
Tissue2
  • Tissue sections were hybridized with 35S-labeled cRNA probes to the rat mu (744-1,064 b), delta (304-1,287 b), and kappa (1,351-2,124 b) receptors. (nih.gov)
  • Its double Z probe design enables an exceptional signal-to-noise ratio when staining formalin-fixed paraffin-embedded (FFPE) tissue specimens. (biospace.com)
Immunohistochemistry1
Cytogenetic1
  • Sensitivity and accuracy are inherent features of these probes , making them a preferred choice for scrutinizing microscopic genetic changes traditionally overlooked by standard cytogenetic tests. (researchandmarkets.com)
Radioactive3
  • Commonly used markers are 32P (a radioactive isotope of phosphorus incorporated into the phosphodiester bond in the probe DNA), digoxigenin, a non-radioactive, antibody-based marker, biotin or fluorescein. (wikipedia.org)
  • Moreover, the quality of these results with a radioactive probe makes it possible to consider using ISH for such rhizospheric applications as localizing and quantifying a microbial invasion of the endorhizosphere or the rhizoplan by using bacterial- or fungal-specific RNA probes, and counting the number of radioactive dots per cell. (agronomy-journal.org)
  • However, major artifacts observed with a digoxegin RNA cold probe makes it necessary to recommend the use of a radioactive RNA probe for these prospects. (agronomy-journal.org)
Nucleic acids1
  • The single session on Thursday was focused on light-driven and fluorescent functionalities and started with Nathan Luedtke talking about biorthogonal fluorescent reporters for nucleic acids in cells and Oliver Seitz focusing on enhanced hybridization probes for RNA imaging. (beilstein-institut.de)
Genome1
  • For this purpose, we applied a global genome-wide probe selection strategy on 32 available complete enterobacterial genomes combined with a regression model for pathogen classification. (biomedcentral.com)
SNPs2
  • We previously developed a highly specific method for detecting SNPs with a microarray-based system using stem-loop probes. (hindawi.com)
  • Hybridization probes targeted these descriptive SNPs and Luminex MagPlex hybridization protocols were developed and optimized. (usda.gov)
Stem-loop probe1
  • We show here that the multiplex genotyping of multiple mutation sites is possible, based on multiplex PCR DNA amplification and a stem-loop probe method. (hindawi.com)
Digoxigenin1
  • The HER-2/neu gene was localized using the Oncor (Gaithersburg, MD) digoxigenin-labeled unique sequence probe. (nih.gov)
Microarrays3
  • Hybridization probes used in DNA microarrays refer to DNA covalently attached to an inert surface, such as coated glass slides or gene chips, to which a mobile cDNA target is hybridized. (wikipedia.org)
  • Besides demonstrating that the probe selection method can be used to study the ovine transcriptome, the benefits of this approach are that it can greatly increase the number of annotated and novel genes that can be studied beyond those currently possible using ovine- or bovine-specific microarrays. (cambridge.org)
  • DNA microarrays based on the selected probes were used to type 92 clinical enterobacterial isolates. (biomedcentral.com)
Diagnostics3
  • Extensive utilization of FISH probes in diagnosing and managing cancerous conditions and genetic disorders, coupled with growth in targeted therapies and In Vitro Diagnostics (IVD) testing, underline the market's expansion potential. (researchandmarkets.com)
  • Our results demonstrate that the global probe selection approach based on longest common factor statistics as well as the design of a DNA microarray with a restricted set of discriminative probes enables robust discrimination of different enterobacterial variants and represents a proof of concept that can be adopted for diagnostics of a wide range of microbial pathogens. (biomedcentral.com)
  • Moreover, a broad range of pathogens have been covered by an efficient probe set size enabling the design of high-throughput diagnostics. (biomedcentral.com)
Sensitivity1
  • Additionally, various technological advancements, such as the development of FISH probes with higher sensitivity and accuracy, are creating a positive outlook for the market. (researchandmarkets.com)
Life Sciences1
  • MINNEAPOLIS , July 6, 2022 /PRNewswire/ -- Bio-Techne (NASDAQ: TECH), a global life sciences company providing innovative tools and bioactive reagents for the research and clinical diagnostic communities today announced the European launch of the CE-IVD marked RNAscope ISH Probe High Risk HPV, intended for use in patients diagnosed with oropharyngeal squamous cell carcinoma (OPSCC) to aid in the identification of high-risk human papillomavirus (HPV). (biospace.com)
Vivo1
  • In order to increase the in vivo stability of the probe RNA is not used. (wikipedia.org)
Demonstrate1
  • In this study, we demonstrate that gDNA-based probe selection on the Affymetrix Human U133+2 GeneChip array can be used to study gene expression profiles in sheep tissues. (cambridge.org)
Silico3
  • New online computational tools for in silico design and testing make it possible to assess candidate FISH probes for S. aureus. (edu.au)
  • Results: A number of new probes for S. aureus were predicted to perform as well or better in silico as those previously reported. (edu.au)
  • In silico evaluation of probes has the potential to reduce the time spent evaluating probes in the laboratory. (edu.au)
Assays1
  • Probes with tethered fluorophore and quencher allow for solution-based assays and eliminate the need for washing steps thereby facilitating integration into microfluidic devices. (psu.edu)
Sequence2
  • These probes are molecules that absorb light of a specific wavelength and emit light upon binding with a particular DNA/RNA sequence. (researchandmarkets.com)
  • Different types of probes may investigate a broad range of sizes of DNA sequence. (msdmanuals.com)
Gene2
  • Gene coexpression analysis refers to the en masse discovery of coexpressed genes from a large variety of transcriptomic experiments. (mdpi.com)
  • Gene probes can be used to locate specific segments of normal or mutated DNA. (msdmanuals.com)
Protocols1
  • Easy to use -With ready-to-use labeled options and multiple optimized protocols available, myTags probes are ideal for new or expert cytogenetics ISH users. (arborbiosci.com)
Molecules2
  • Natural and artificial functionalities as well as probes, markers or other biologically active molecules can be introduced synthetically into DNA, and in the meantime also into RNA, by preparing the corresponding artificial DNA building blocks. (beilstein-institut.de)
  • This is especially important for functionalities, probes or biologically relevant molecules that are not compatible with the routinely applied phosphoramidite chemistry. (beilstein-institut.de)
Multiplex1
  • This system now combines the principle of highly specific genotyping based on stem-loop structure probes with the advantages of multiplex analysis. (hindawi.com)
Specific5
  • On the contrary, it is plausible that there exist microorganisms, yet to be identified, which are phylogenetically members of a probe target group, but have partial or near-perfect target sites, usually applies when designing group-specific probes. (wikipedia.org)
  • myTags Custom probes have been demonstrated to achieve highly specific targeted F(ISH) across a wide range of applications and species. (arborbiosci.com)
  • We previously developed a highly specific genotyping method based on hairpin probes. (hindawi.com)
  • Our probes, also called hairloops, consist of a GC-rich stem and a target-specific loop [ 15 ]. (hindawi.com)
  • Amplified products were hybridized to different specific probes fixed as a line on membrane strips and visualized using a straptavidine /alkaline phosphatase conjugated system. (bvsalud.org)
Methods2
  • Depending on the method, the probe may be synthesized using the phosphoramidite method, or it can be generated and labeled by PCR amplification or cloning (both are older methods). (wikipedia.org)
  • Materials and Methods: Six online tools, NCBI-Nucleotide, Ribosomal Database Project, NCBI-Blast, Reverse-Complement, Probecheck, and mathFISH, were employed in a workflow to evaluate FISH probes for S. aureus. (edu.au)
Locus1
  • Comparing myTags synthetic probes (a) versus BAC-derived probes (b) for the human TP53 locus [ATTO 550] demonstrates that the myTags probes only hybridize to the intended locus, while the BAC-derived probes also display multiple off-target hybridizations, denoted by asterisks in (b). (arborbiosci.com)
Bacterial1
  • The probe did not hybridize to the PCR products from Xanthomonas albilineans, the leaf scald bacterial pathogen, other Xanthomonads, and saprophytic bacteria commonly found in sugarcane. (usda.gov)
Conventional3
  • By confining FISH probes at the tip of this microfabricated scanning probe, we locally exposed approximately 1000 selected MCF-7 cells of a monolayer to perform incubation of probes - the rate-limiting step in conventional FISH. (springer.com)
  • This method is compatible with the standard workflow of conventional FISH, allows re-budgeting of the sample for various tests, and results in a ~ 15-fold reduction in probe consumption. (springer.com)
  • a Using a pipette in conventional FISH, CEP7 probes are deposited on the entire cell monolayer. (springer.com)
Analysis2
  • We further demonstrated spatially multiplexed μFISH, enabling the use of spectrally equivalent probes for detailed and real-time analysis of a cell monolayer, which paves the way towards rapid and automated multiplexed FISH on standard cytological supports. (springer.com)
  • The results of MSRF were confirmed by southern hybridization analysis using the aberrantly methylated DNA fragments as the probes. (cdc.gov)
Products1
  • One of the major limitations of our original method was the use of individual PCR products, amplified separately, for the hybridization and genotyping reaction. (hindawi.com)
Previously1
  • A previously reported probe, Staaur-16S69, was compared to a new probe, KT18-16S68, predicted by mathFISH to have the same performance. (edu.au)