Epithelial and mesenchymal cells differ in phenotype as well as function, though both share inherent plasticity. (wikipedia.org)
Epithelial cells are closely connected to each other by tight junctions, gap junctions and adherens junctions, have an apico-basal polarity, polarization of the actin cytoskeleton and are bound by a basal lamina at their basal surface. (wikipedia.org)
Epithelial cells express high levels of E-cadherin, whereas mesenchymal cells express those of N-cadherin, fibronectin and vimentin. (wikipedia.org)
We show that single epithelial stem cells in vitro can give rise to differentiated organoids containing ciliated and secretory cells. (nature.com)
Here we demonstrate the existence of adult stem cells in the human fallopian tube epithelium, which gives rise to a monolayer of differentiated epithelial cells in a complex 3D organoid in vitro . (nature.com)
Ki-67-positive cells were distributed along the epithelial folds, without restriction to a particular region with occasional clustering ( Fig. 1a , asterisk). (nature.com)
Figure 1: Single fallopian epithelial cells give rise to organoids that can be maintained long term. (nature.com)
These toxins bind to specific receptors of the intestinal epithelial cells and cause secretion of water and electrolytes into the intestinal lumen. (cdc.gov)
Triple negative breast cancer cells also gain survival advantages by producing a protein called E-cadherin, typically found in epithelial cells that line the ducts and coverings of organs and frequently renew themselves. (sciencedaily.com)
When triple negative breast cancer cells gain such survival and migratory qualities, scientists classify their cellular state as so-called hybrid epithelial mesenchymal (EMT) cells. (sciencedaily.com)
To look more closely at molecules involved in hybrid EMT states, the scientists sought the help of Elana Fertig, Ph.D., division director and associate director of quantitative sciences and co-director of the Convergence Institute at the Johns Hopkins Kimmel Cancer Center, to track the molecular patterns of individual cells in cell assays that model invasion out of the primary tumor and formation of a colony in a metastatic site. (sciencedaily.com)
The scientists found that most of the metastatic cells morph into the more mobile, better surviving, hybrid EMT state. (sciencedaily.com)
This indicates that TDI effect on ion transport in epithelial cells depends on C fibresand tachykinins released from their endings. (medscimonit.com)
We are particularly interested in studying the outer retina, consisting of photoreceptor neurons, retinal pigment epithelial cells, and choriocapillaris blood vessels. (nih.gov)
This multi-layered complex is not only critical for the phenomenon of vision, but also, is a useful system for modeling the in vivo interactions of neurons, epithelial cells, and vasculature within the central nervous system, in health, aging, and disease. (nih.gov)
The EU is investing the equivalent of just over SEK 32 million in recruiting ten doctoral students to various European research groups studying how epithelial cells build up contact surfaces in different organs that handle bacteria. (akademiliv.se)
The network aims to understand how epithelial cells, for example those in the gut, organize themselves to absorb vital nutrients while also keeping out harmful microorganisms such as bacteria. (akademiliv.se)
These diseases are triggered by bacteria coming into direct contact with the epithelial cells of the gut, but the underlying causes and susceptibilities are completely unknown. (akademiliv.se)
If we can make good progress toward creating a good artificial system with human cells and artificial mucus that resembles the native mucus in our bodies, this would provide better conditions for studying the interactions between epithelial cells and bacteria," adds Thaher. (akademiliv.se)
ADAM28 was immunolocalized primarily in epithelial cells of several tissues, including epididymis, bronchi, and stomach, while lymphocytes from lymph nodes and spleen were immunostained negligibly. (enigmadiagnostics.com)
C1q treatment of normal BEAS-2B and NHBE bronchial epithelial cells, both of which showed low expression of ADAM28, caused apoptosis by activation of p38 and caspase-3, and cell death with autophagy by accumulation of LC3-II and autophagosomes, respectively. (enigmadiagnostics.com)
These data demonstrate that ADAM28 is expressed by epithelial cells of several normal organs and suggest that ADAM28 plays a role in cell survival by suppressing C1q-induced cytotoxicity in bronchial epithelial cells. (enigmadiagnostics.com)
However, little is known about the molecular changes that these viral agents may induce in the prostate epithelial cells that might lead to the initiation or progression of prostate cancer. (researchsquare.com)
The abnormalities observed on a cytologic smear or tissue biopsy of the cervix represent alterations in the degree of differentiation of cervical epithelial cells. (justia.com)
In some embodiments, compositions and methods of the invention are useful for treating mucosal conditions (e.g., diseases and/or infections of mucosal tissue, for example of mucosal epithelial cells). (justia.com)
Thus, APN mediates the attachment of F4(+) E. coli to intestinal epithelial cells. (k-state.edu)
The bifunctional molecules were tested on human erythrocytes, GECs (gingival epithelial cells) and GFCs (gingival fibroblast cells) for cytotoxicity. (marquette.edu)
Keratins are a large family of proteins which form the intermediate filament (IF) cytoskeleton of epithelial cells and their appendages hairs and nails (reviewed in references 8 and 15). (exposed-skin-care.net)
At the same time there is little data on the properties of circulating cells showing simultaneous expression of the leukocyte and epithelial markers and their possible role in tumor progression and chemotherapy resistance. (rsmu.press)
The study was aimed to assess subpopulations of cells with hybrid epithelial/leukocyte phenotype and estimate the features of stemness, epithelial-mesenchymal transition (EMT), and integrin interface, which determine the cells' possible metastatic properties in breast cancer (BC). (rsmu.press)
Multicolor flow cytometry was used to assess the population structure and metastatic potential of the cells circulating in blood and primary tumor cells with hybrid phenotype. (rsmu.press)
The high degree of heterogeneity was noted in the population of cells with hybrid phenotype, including the combination of the stemness and EMT features, and diverse integrin interface. (rsmu.press)
Cells with hybrid phenotype are involved in the mechanisms underlying lymph node and distant metastasis. (rsmu.press)
In distant metastasis, metastatic potential of hybrid cells is associated with the stemness features ( р = 0.015) and is not associated with the EMT features and integrin expression. (rsmu.press)
Loss of the Metastasis Suppressor NME1, But Not of Its Highly Related Isoform NME2, Induces a Hybrid Epithelial-Mesenchymal State in Cancer Cells. (masader.om)
Following the formation of a primary carcinoma, neoplastic cells metastasize by undergoing the epithelial mesenchymal transition (EMT), which is triggered by cues from inflammatory and stromal cells in the microenvironment. (biomedcentral.com)
EMT allows epithelial cells to lose their highly adhesive nature and instead adopt the spindle-like appearance, as well as the invasive and migratory behavior, of mesenchymal cells. (biomedcentral.com)
Characteristically, epithelial cells form polarized sheets. (biomedcentral.com)
This E-cadherin-catenin complex is what gives the epithelial cells in both normal tissue and carcinomas their strong adhesive bonds and inhibits the movement of individual cells [ 2 , 4 ]. (biomedcentral.com)
Their proof-of-principle for generating human tissue is demonstrated by 3D bioprinting human airways composed of regionally specified primary human airway epithelial progenitor and smooth muscle cells. (lu.se)
Airway lumens remained patent with viable cells for one month in vitro with evidence of differentiation into mature epithelial cell types found in native human airways. (lu.se)
Acquisition of cancer stem cell-like properties in human small airway epithelial cells after a long-term exposure to carbon nanomaterial s. (cdc.gov)
Cells which lack ER expression at diagnosis arise from an ER compart- were harvested at 60 - 80% confluency and used as a reference in all hybrid- ment within the mammary epithelium or represent evolution from an izations. (lu.se)
Transition5
Epithelial-mesenchymal transition was first recognized as a feature of embryogenesis by Betty Hay in the 1980s. (wikipedia.org)
EMT, and its reverse process, MET (mesenchymal-epithelial transition) are critical for development of many tissues and organs in the developing embryo, and numerous embryonic events such as gastrulation, neural crest formation, heart valve formation, secondary palate development, and myogenesis. (wikipedia.org)
Current interest in this process, which is described as an epithelial-mesenchymal transition (EMT), stems from its developmental importance and its involvement in several adult pathologies. (rupress.org)
The EMT International Association was formed in 2002 to provide an international body for those interested in EMT and the reverse process, mesenchymal-epithelial transition, and, most importantly, to bring together those working on EMT in development, cancer, fibrosis, and pathology. (rupress.org)
Notch signaling has been implicated in many microenvironmental processes associated with cancer progression such as vascular remodeling, immunosuppression, epithelial to mesenchymal transition (EMT), as well as maintenance of the CSC pool 21 . (nature.com)
Carcinoma1
The model predicts that following activation of the Wnt pathway, an epithelial cell in the primary carcinoma must attain a threshold level of membrane-bound Dvl to convert to the mesenchymal-like phenotype and maintain that phenotype once it has migrated away from the primary tumor. (biomedcentral.com)
Differentiation1
The close histopathologic and immunohistochemical resemblance among adamantinomatous craniopharyngioma, adamantinoma of the jaw, and calcifying odontogenic cyst suggests an odontogenic epithelial differentiation for these tumors. (medscape.com)
Metastasis1
The team found that cancer metastasis has three states: "on," "off," and a partial on-off state known as a "hybrid. (medicaldaily.com)
Cancers2
The p63 factor is involved in inhibiting EMT and reduction of certain p63 isoforms may be important in the development of epithelial cancers. (wikipedia.org)
Its P-MUC1C-ALLO1 is an allogeneic CAR-T product candidate with the potential to treat a range of solid tumor indications, including breast and other epithelial-derived cancers. (investing.com)
Tumors2
Craniopharyngiomas are low-grade (World Health Organization [WHO] grade I) circumscribed epithelial tumors that most commonly arise in the suprasellar region. (medscape.com)
Epithelial tumors, or carcinomas, are the most common type of neoplasia found in humans, accounting for roughly 80% of all cancer related deaths in the Western world [ 1 ]. (biomedcentral.com)
Cell8
The conversion of an epithelial cell to a mesenchymal cell is critical to metazoan embryogenesis and a defining structural feature of organ development. (rupress.org)
The Company's approach to cell and gene therapies is based on its genetic editing platforms, including its non-viral piggyBac DNA Delivery System, Cas-CLOVER Site-Specific Gene Editing System and nanoparticle and hybrid gene delivery technologies. (investing.com)
Patients at greatest risk of persistent epithelial defect after Boston KPro are those with preoperative corneal limbal stem cell deficiency. (masseyeandear.org)
From a structural point of view we have recently reported that different keratin polypeptides even those belonging to the same expression pair display distinct dynamics in cell hybrids (19). (exposed-skin-care.net)
Epithelial cell cluster size affects force distribution in response to EGF-induced collective contractility. (mpg.de)
Tuning epithelial cell-cell adhesion and collective dynamics with functional DNA-E-Cadherin hybrid linkers. (mpg.de)
We hypothesize that a bistable switch between the epithelial and mesenchymal phenotypes governs EMT, allowing the cell to maintain its mesenchymal phenotype even after it leaves the primary tumor microenvironment and EMT-inducing extracellular signal. (biomedcentral.com)
This highly adhesive nature is attributable to molecules of E-cadherin, a transmembrane protein, on the surface of one epithelial cell binding with the E-cadherin moelcules of another epithelial cell across the extracellular space. (biomedcentral.com)
Cervical1
Early clini- fication scheme for tumours of the atypical transformation zone, usually cal invasive disease (stage IB1) may uterine cervix recognizes three general by colposcopy in developed countries be considered for radical cervical categories of epithelial tumours: squa- in the presence of a report showing removal as described for stage IA2. (who.int)
a hybrid odontogenic tumor with a combination of the calcifying epithelial odontogenic tumor (CEOT) is present, adenomatoid odontogenic tumor (AOT) and calcifying odontogenic cyst (COC), located in the anterior ma. (uitm.edu.my)
An aggressive presentation of adenomatoid odontogenic tumor associated with calcifying epithelial odontogenic tumor: A hybrid variant by: Shubhangi Mhaske, et al. (uitm.edu.my)
Hybrid Odontogenic Tumor of Calcifying Odontogenic Cyst and Ameloblastic Fibroma: a Case Report and Review of Literature by: Nazanin Mahdavi, et al. (uitm.edu.my)
Tissues1
The present study investigateda possible effect of TDI on ion transport using electrophysiological methods aimed at assessment of ioncurrents occurring in epithelial tissues. (medscimonit.com)
Tumor1
However, on the other hand, p53, a well-known tumor suppressor, represses EMT by activating the expression of various microRNAs - miR-200 and miR-34 that inhibit the production of protein ZEB and SNAIL, and thus maintain the epithelial phenotype. (wikipedia.org)
Ovarian2
The epithelial lining of the fallopian tube is of critical importance for human reproduction and has been implicated as a site of origin of high-grade serous ovarian cancer. (nature.com)
Prevalence of germline pathogenic BRCA1/2 variants in sequential epithelial ovarian cancer cases. (cdc.gov)
Lung1
C1q was identified as a candidate ADAM28-binding protein from a human lung cDNA library by a yeast two-hybrid system, and specific binding was demonstrated by binding assays, immunoprecipitation and surface plasmon resonance. (enigmadiagnostics.com)
Protein1
Two-hybrid techniques refer to analysis for protein-protein interactions, one-hybrid for DNA-protein interactions, three-hybrid interactions for RNA-protein interactions or ligand-based interactions. (uchicago.edu)
Organs1
MATERIAL/METHODS: The experiments were carried out on 119 fragmentsof isolated frog skin, sampled from 59 specimens of hybrid frog Rana esculenta L. The procedures employedinvolved transepithelial electrical potential (PD in mV) measurement with an Ussing apparatus, modifiedto enable mechanical stimulation of organs and defined pharmacological actions. (medscimonit.com)
Interactions1
Reverse n-hybrid techniques refer to analysis for mutations or other small molecules that dissociate known interactions. (uchicago.edu)
Assays2
Here we show in both yeast two-hybrid and pulldown assays that APN binds directly to FaeG, the major subunit of F4 fimbriae, from three serotypes of F4(+) ETEC. (k-state.edu)
The most dominant techniques are PCR viral detection and signal amplification DNA-based assays (Hybrid Capture II is the only FDA-approved technique at present). (medscape.com)
Extracellular1
Here, a tissue-specific hybrid bioink is described, composed of a natural polymer, alginate, reinforced with extracellular matrix derived from decellularized tissue (rECM). (lu.se)
Cellular1
and CIN III refers to cellular dysplasia encompassing greater than two-thirds of the epithelial thickness. (justia.com)
Molecules1
This was linked through the side chain of the N-terminal residue to the C-terminus of a defensin-like 12-residue peptide to generate two bifunctional hybrid molecules, one with an ester linkage and the other with an anhydride bond between the carrier and the antimicrobial components. (marquette.edu)
Coli1
These data thus reveal that the emergence of edema disease in wild boars was caused by atypical hybrid of STEC and enterotoxigenic E. coli O139:H1, which so far has been restricted to the wildlife environment. (cdc.gov)
Keratin1
Although the primary function of keratin PD1-PDL1 inhibitor 2 PD1-PDL1 inhibitor 2 IF has long been thought of as structural this hypothesis was not confirmed until the discovery that keratin mutations result in diseases characterized by epithelial fragility (reviewed in references 3 5 6 and 15. (exposed-skin-care.net)
Suggests1
Furthermore, sensitivity analysis of the model suggests that in both the epithelial and the mesenchymal states, the steady state behavior of E-cadherin and the transcription factor Slug are sensitive to changes in the degradation rate of Slug, while E-cadherin is also sensitive to the IC 50 (half-maximal) concentration of Slug necessary to inhibit E-cadherin production. (biomedcentral.com)
Human2
In human whole saliva, a sustained antimicrobial release over a period of more than 40-60 h, and candidacidal activity consistent with the extent of hybrid dissociation were observed. (marquette.edu)
The hybrid peptides did not show any toxic influence on human erythrocytes, GECs and GFCs. (marquette.edu)
Somatic1
Funk and co-investigators localized COX-1 to 9q32-q33.3 via somatic hybrid deoxyribonucleic acid (DNA) analysis. (medscape.com)
Expression1
Snail and Slug are known to regulate the expression of p63 isoforms, another transcription factor that is required for proper development of epithelial structures. (wikipedia.org)
Fiber1
Nitrogen-doped CNT on CNT hybrid fiber as a current collector for high-performance Li-ion capacitors. (cdc.gov)
Surface1
The hybrid-adsorbed HAP surface was examined against Candida albicans and Aggregatibacter actinomycetemcomitans using the fluorescence technique. (marquette.edu)