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  • vitro
  • Aim of study: Analysis of in vitro and in vivo growth properties, differential chromosomal aberrations and differential gene expression patterns of non-tumorigenic hybrid cells compared to tumorigenic segregants and tumorigenic parental HeLa cells by cDNA microarray analysis. (aacrjournals.org)
  • Results: Confirmation of similar expression levels of HPV18 viral oncogenes and in vitro and in vivo growth properties in non-tumorigenic and tumorigenic cells. (aacrjournals.org)
  • Derivation, characterization, and in vitro differentiation of canine embryonic stem cells. (biomedsearch.com)
  • Cell fusion has proven to be an useful tool for studying the genetic control of neoplastic transformation and its modulation in vitro (for review see, 1-4 ). (springer.com)
  • HeLa
  • Here we focus on hybrid cells after fusion of tumorigenic HPV18-positive HeLa cells and normal human fibroblasts. (aacrjournals.org)
  • Materials and Methods: Differential gene expression patterns were analysed in non-tumorigenic and tumorigenic hybrid cells, parental HeLa cells and normal human fibroblasts by microarray analysis using Hu133Plus2.0 chips by Affymetrix. (aacrjournals.org)
  • The two cell types studied in the first instance were the HeLa cell (a cell of human origin which has been grown for many years in artificial culture) and the Ehrlich ascites cell (a tumour which grows as a cell suspension in the peritoneal cavity of the mouse). (royalsocietypublishing.org)
  • dendritic
  • This is actually the first cell we know of that has this type of appearance in nature," Dr. Andrew Mellor, molecular geneticist and immunologist who co-directs MCG's Immunotherapy Discovery Institute, said of the cell that looks like a dendritic cell and a B cell but isn't really either. (bio-medicine.org)
  • When MCG scientists first reported the human equivalent of this cell in Science in 2002, they called it a subset of the dendritic cell that clusters in high exposure areas such as the gut but also roams the body, looking for invaders like a virus or cancer. (bio-medicine.org)
  • Dendritic cells show their find to T cells, telling them to ignore or attack by bringing trash-eating macrophages, natural killer cells and the like into the fight. (bio-medicine.org)
  • It looks like a dendritic cell and it is and it isn't," Johnson said. (bio-medicine.org)
  • They also may be showing up in new dendritic cell therapies designed to strengthen the immune response to cancer. (bio-medicine.org)
  • humans
  • While their studies are in mice, the cells also are in humans, showing up in some unfortunate places such as the drainage system for tumors, melanoma or even HIV where they likely help the diseases survive. (bio-medicine.org)
  • Recombinant DNA Humans and Hybrids: A Critique of the Western Moral Framework Junca et al. (wikipedia.org)
  • neuroblastoma
  • Neuroblastoma x spinal cord (NSC) hybrid cell lines resemble developing motor neurons. (nih.gov)
  • We have developed a series of mouse-mouse neural hybrid cell lines by fusing the aminopterin-sensitive neuroblastoma N18TG2 with motor neuron-enriched embryonic day 12-14 spinal cord cells. (nih.gov)
  • analyses
  • Thomas Cremer (born July 7, 1945 in Miesbach, Germany ), is a German professor of human genetics and anthropology with a main research focus on molecular cytogenetics and 3D/4D analyses of nuclear structure studied by fluorescence microscopy including super-resolution microscopy and live cell imaging. (wikipedia.org)
  • fuse
  • The University of Colorado Cancer Center along with Yale University and the Denver Crime Lab report in the journal PLoS ONE the first proof of cancer's ability to fuse with blood cells in a way that gives cancer the ability to travel, allowing previously stationary cancer cells to enter the bloodstream and seed sites of metastasis around the body. (innovations-report.com)
  • We showed that an animal virus, killed by irradiation with ultraviolet light, could be used to fuse together cells derived from mouse and man to produce artificial man-mouse hybrid cells. (royalsocietypublishing.org)
  • It was thus a very small step to attempt to see whether a virus could be used to fuse together cells of different kinds, and whether the resulting hybrid cells, if they were formed, would survive. (royalsocietypublishing.org)
  • but the dead virus retained its ability to fuse cells together. (royalsocietypublishing.org)
  • Cells from the same organism may fuse together as well. (wikipedia.org)
  • The term cell fusion mechanism refers to the receptors required for cells to fuse, whereas the term cell fusion refers to the action of formation of the new cell. (wikipedia.org)
  • In order for HIV to fuse with a cell, it must be able to bind to the receptors CD4, CCR5, and CXCR4. (wikipedia.org)
  • fuel cell vehicles
  • This leasing, as well as TMC's participation in other cooperative efforts with the government, the energy industry and other parties concerned is aimed at helping to bring about the widespread use of fuel cell vehicles. (automobilesreview.com)
  • On June 4, 2014, Toyota announced plans to begin production of Toyota Mirai fuel cell vehicles by mid-December 2014. (wikipedia.org)
  • The FCHV-3 was presented at the "International Symposium on Fuel-Cell Vehicles" in March 2001 in Tokyo, and the FCHV-4 was the first production version, although Toyota did not plan a full-scale commercial hydrogen fuel cell vehicle launch until around 2015. (wikipedia.org)
  • donor
  • The work used DNA fingerprinting of a bone marrow transplant patient with cancer, along with DNA fingerprinting of the patient's bone marrow donor, to show that subsequent metastatic cancer cells in the patient's body carried parts of both genomes, fused together into a hybrid cancer cell. (innovations-report.com)
  • He had received a pathology specimen of a melanoma tumor that had metastasized to the brain of a patient who had previously received a bone marrow transplant from his brother, and he asked me whether, as a collaboration, we could distinguish between the donor and patient genomes in a cancer cell? (innovations-report.com)
  • After much dedicated work to this single tumor, they found that the entire tumor was populated by cancer cell hybrids with genomes containing both donor and patient DNA. (innovations-report.com)
  • Hybrid photovoltaics have organic materials that consist of conjugated polymers that absorb light as the donor and transport holes. (wikipedia.org)
  • protein
  • Oct4-green fluorescent protein (GFP) transgenes that do not contain the proximal enhancer (PE) region are widely used to visualize reprogramming of the somatic to the pluripotent cell state. (biomedsearch.com)
  • Researchers at the Max Planck Institute of Biochemistry (MPIB) have demonstrated that actin, the most abundant protein in higher developed cells, does not have the inbuilt potential to fold and instead requires special assistance to fold into its active state. (innovations-report.com)
  • D-Ala2,D-Leu enkephalin , which decreased cyclic AMP levels and reversed the 2-chloroadenosine-stimulated phosphorylation of tyrosine hydroxylase in differentiated PC12 cells, also reversed the stimulation of phosphorylation of the 90-kDa protein in NCB-20 cells. (curehunter.com)
  • Immunological quantification of proteoglycan core protein on treated cells suggests that the cells have an unchanging number of this proteoglycan at their cell surface. (rupress.org)
  • Analysis of the glycosaminoglycan composition of the proteoglycan shows an increased amount of chondroitin sulfate, suggesting that the increased labeling per cell may be attributed to an augmented synthesis of chondroitin sulfate glycosaminoglycan on the core protein that also bears heparan sulfate, thus altering the proportions of these two glycosaminoglycans on this hybrid proteoglycan. (rupress.org)
  • Molecular
  • Molecular, polymer, and hybrid organic photovoltaics are the main kinds of organic photovoltaic devices currently studied. (wikipedia.org)
  • Cloning in biotechnology refers to processes used to create copies of DNA fragments (molecular cloning), cells (cell cloning), or organisms (organism cloning). (wikipedia.org)
  • polymer
  • The combination of inorganic nanoparticles semiconductor, conjugated polymer, and dye-sensitized in a layer of solar cell is now recognized as potential application in developing flexible, large area, and low cost photovoltaic devices. (hindawi.com)
  • This review focuses on developing solid-state dye-synthesized, polymer, and hybrid solar cells. (hindawi.com)
  • By leasing its fuel cell hybrid vehicles, TMC can obtain real-world feedback for further development, including efforts to improve the durability and reduce the cost of its proprietary high-performance polymer electrolyte fuel cell, the Toyota FC Stack. (automobilesreview.com)
  • induce
  • In addition, NSC-34 cells induce acetylcholine receptor clusters on cocultured myotubes, and undergo a vimentin-neurofilament switch with maturation in culture, similar to that occurring in neuronal development. (nih.gov)
  • The implications of this concept are, first, only MHC class I and II expressing cells can induce cytolytic T-cell responses ( 5 -7 ), second, cognate antigens must be presented for both T-cell and MHC types ( 4 ,8 ) and, third, there must be T cells with the corresponding specificities in the T-cell receptor repertoire of the response-competent individual. (springer.com)
  • Chemical
  • A number of alternative techniques are available, such as chemical sensitivation of cells, electroporation, optical injection and biolistics. (wikipedia.org)
  • expression
  • Increased expression of unknown genes in tumorigenic compared to non-tumorigenic hybrid cells: eg. (aacrjournals.org)
  • We have examined the ability of mouse mammary epithelial (NMuMG) cells to respond to TGF-beta and assessed the effect of the growth factor on the expression of their cell surface heparan sulfate/chondroitin sulfate hybrid proteoglycan. (rupress.org)
  • K. K. Jha, J. Cacciapuoti, and H. L. Ozer, Expression of transformation in cell hybrids. (springer.com)
  • efficiency
  • Mitchison, N. A. (1990) An exact comparison between the efficiency of two-and three-cell-type clusters mediating helper activity. (springer.com)
  • The BC Transit fleet is the first that incorporates Ballard's heavy-duty fuel cell module into a hybrid fuel cell / battery architecture with an electric drive, which enables higher vehicle efficiency and improved fuel cell durability. (altenergymag.com)
  • Ballard's heavy duty fuel cell module, the FCvelocity®-HD6, delivers enhanced fuel cell durability and improved efficiency at a reduced cost, while offering an industry leading 12,000 hours/five year warranty. (altenergymag.com)
  • Controlling the interface of inorganic-organic hybrid solar cells can increase the efficiency of the cells. (wikipedia.org)
  • Mesoporous films have been used for a relatively high-efficiency hybrid solar cell. (wikipedia.org)
  • As the aforementioned procedures are of particularly low efficiency, there is a need to identify the cells that have been successfully transfected with the vector construct containing the desired insertion sequence in the required orientation. (wikipedia.org)
  • precursor
  • It is based on the observation that epitope linkage is a prerequisite for productive T-T cell collaboration, i.e., cytolytic precursor and helper T cells have to be activated by the same antigen presenting cell that displays epitopes for both T-cell types on the corresponding MHC class I and class II molecules ( 3 ,4 ). (springer.com)
  • In fact, they found the IDO-presenting cells came from the same precursor cell as B cells. (bio-medicine.org)
  • Hydrogen
  • Concept cars have been made (the Honda FX Clarity, the Chevrolet Equinox Fuel Cell, BMW Hydrogen 7 and Cadillac Provoq) but it will be years before we see fuel cell cars on the road. (greenanswers.com)
  • BC Transit's fleet of 20 hydrogen fuel cell buses is the largest single deployment of zero-emission fuel cell buses worldwide. (altenergymag.com)
  • The two crucial pieces of the system are the Fuel Cell Stack and the Hydrogen Recirculation Device. (altenergymag.com)
  • The durability and performance of the Fuel Cell Stack depends on the durability and performance of the Hydrogen Recirculation Device. (altenergymag.com)
  • Moving hydrogen with a rotary pump has its advantages in smaller size, lighter weight and a lower parasitic electrical load for the Fuel Cell. (altenergymag.com)
  • The powertrain consists of a 90 kW fuel cell supplied from onboard compressed hydrogen tanks, and a nickel-metal hydride battery in parallel. (wikipedia.org)
  • 90 kW PEFC Fuel cell stack: twice Motor: AC synchronous 80 kW twice Hydrogen tank: Compressed hydrogen gas 35 MPa / 150 liter, five (version 2002) or seven (version 2005) Passenger capacity: 63 (included 22 seats) FCHV-BUS demonstrated at Toei Bus (August 2003 - December 2004) and Expo 2005. (wikipedia.org)
  • antigenic
  • Nonetheless, metabolic labeling with radiosulfate shows a approximately 2.5-fold increase in 35SO4-glycosaminoglycans in this proteoglycan fraction, defined either by its lipophilic, antigenic, or cell surface properties. (rupress.org)
  • clusters
  • Mitchison, N. A. and O'Malley, C. (1987) Three-cell-type clusters of T cells with antigen-presenting cells best explain the epitope linkage and noncognate requirements of the in-vivo cytolytic response. (springer.com)
  • human
  • When MCG scientists first reported the human equivalent of this cell i. (bio-medicine.org)
  • P. A. Jones, J. S. Rhim, and H. Isaacs, The relationship between tumorigenicity, growth in agar and fibrinolytic activity in a line of human osteosarcoma cells. (springer.com)
  • L. Larizza, G. Simoni, M. Stefanini, and L. De Carli, Spontaneous and Griseofulvin induced segregation for 8-Azaguanine resistance in hybrids from a human heteroploid line. (springer.com)
  • The hybrid cells continued secretion of mouse immunoglobulin and initiate synthesis and secretion of human immunoglobulin. (wikipedia.org)
  • photovoltaic
  • Several conjugated low bandgap polymers, dyes, and underlayer materials based on the previous studies are quoted in this paper, which can provide guidelines in designing low cost photovoltaic solar cells. (hindawi.com)
  • The hybrid photovoltaic devices have a potential for not only low-cost by roll-to-roll processing but also for scalable solar power conversion. (wikipedia.org)
  • Solar cells are devices that convert sunlight into electricity by the photovoltaic effect. (wikipedia.org)
  • organisms
  • However, in the case of cell cultures from multi-cellular organisms, cell cloning is an arduous task as these cells will not readily grow in standard media. (wikipedia.org)
  • solar
  • Hybrid solar cells combine advantages of both organic and inorganic semiconductors. (wikipedia.org)
  • Electrons in a solar cell absorb photon energy in sunlight which excites them to the conduction band from the valence band. (wikipedia.org)
  • Organic solar cells use organic materials in their active layers. (wikipedia.org)
  • In hybrid solar cells, an organic material is mixed with a high electron transport material to form the photoactive layer. (wikipedia.org)
  • The structure of mesoporous thin film solar cells usually includes a porous inorganic that is saturated with organic surfactant. (wikipedia.org)
  • Researchers have been able to grow nanostructure-based solar cells that use ordered nanostructures like nanowires or nanotubes of inorganic surrounding by electron-donating organics utilizing self-organization processes. (wikipedia.org)
  • immune
  • This immune therapeutical approach aims at recruitment of T-cell help for the induction of tumor-specific cytolytic immunity. (springer.com)
  • AUGUSTA, Ga. - A cell small in number but powerful in its ability to switch the immune system on or off is a unique hybrid of two well-known immune cell types, Medical College of Georgia researchers report. (bio-medicine.org)
  • Their confusing face could also cause hybrids to be lost in B cell-depleting therapies designed to lessen the immune system's attack on joints in rheumatoid arthritis. (bio-medicine.org)
  • HIV infects by fusing with the membranes of immune system cells. (wikipedia.org)
  • tumour
  • Stuhler, G. and Walden, P. (1994) Recruitment of helper T-cells for induction of tumour rejection by cytolytic T lymphocytes. (springer.com)
  • Sendai virus was chosen because it had been shown by Okada (1958, 1962) that animal tumour cells in suspension could be rapidly fused together by high concentrations of this virus. (royalsocietypublishing.org)
  • gene
  • Studies in rat suggested that this gene may be an immediate-early gene in mitogen-stimulated cells. (wikipedia.org)
  • Melanoma
  • The treatment, which inhibits the division of cancer cells, is aimed at patients in later stages of kidney cancer or melanoma. (wikipedia.org)