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  • antibodies
  • They plan on accomplishing this by building upon techniques developed by Dr. Nussenzweig for the cloning of anti-HIV human antibodies from single cells. (cavd.org)
  • With respect to affinity isolations, the triple-FLAG (3×FLAG)-tag exhibits superiority to the single FLAG-tag through increased avidity of interaction with M2 anti-FLAG antibodies ( 25 - 27 ). (pubmedcentralcanada.ca)
  • Polyreactive human pneumococcal polysaccharide IgG antibodies have not been extensively studied. (scirp.org)
  • We analyzed human polyreactive antibodies that bind multiple pneumococcal polysaccharides, including PPS14 and PPS23F. (scirp.org)
  • These antibodies were isolated from single pneumococcal polysaccharide specific B cells allowing for the analysis of human immunoglobulins with natively paired variable regions. (scirp.org)
  • Carmela Dantas-Barbosa, Fabrícia P. Faria, Marcelo M. Brigido, and Andrea Q. Maranhão, "Isolation of Osteosarcoma-Associated Human Antibodies from a Combinatorial Fab Phage Display Library," Journal of Biomedicine and Biotechnology , vol. 2009, Article ID 157531, 8 pages, 2009. (hindawi.com)
  • Isolation of human antibodies to tumor-associated endothelial cell markers by in vitro human endothelial cell selection with phage display libraries," Journal of Immunological Methods , vol. 287, no. 1-2, pp. 31-47, 2004. (hindawi.com)
  • The aim of this study was to identify individuals with serum reactivity against the central and COOH-terminal domains of p53 and to isolate and characterize these recombinant human antibodies. (aacrjournals.org)
  • Human antibodies have been isolated against a number of tumor antigens, including p53 (18) , and these human anti-p53 antibodies were shown to bind with high affinity to an epitope between residues 27 and 44 of the NH 2 terminus of p53. (aacrjournals.org)
  • The binding of each of the human monoclonals was inhibited by GAD6 F(ab')2 and the binding of GAD6 antibody was inhibited by the human monoclonal F(ab')2s suggesting that the epitopes for these antibodies were overlapping. (epfl.ch)
  • affinity
  • An efficient and reliable procedure for the capture of affinity-tagged proteins and associated complexes from human cell lines is reported. (pubmedcentralcanada.ca)
  • Through multiple optimizations, high yield and low background affinity-purifications are achieved from modest quantities of human cells expressing endogenous-level tagged proteins. (pubmedcentralcanada.ca)
  • Affinity isolation of proteins and associated complexes has facilitated the rapid growth of proteomic analyses ( 1 ). (pubmedcentralcanada.ca)
  • Simultaneously, the technologies and methods for cell breakage and affinity isolation have themselves evolved. (pubmedcentralcanada.ca)
  • Our laboratory has shown that near complete cell breakage can be achieved by mechanical grinding at liquid N 2 temperatures, resulting in a fine, granular material (cell grindate) with excellent properties for subsequent affinity isolation of tagged proteins and complexes ( 22 - 24 ) (e.g., http://www.ncdir.org/wp-content/uploads/grinding.mp4 ). (pubmedcentralcanada.ca)
  • Production of human monoclonal autoantibodies to glutamic acid decarboxylase Mr 65,000 (GAD65), characterization of their isotype, binding affinity, V region sequences and competition with autoantibodies in patients' sera is described. (epfl.ch)
  • peripheral
  • In the wake of collaborations with the Stanford School of Medicine and various industry partners, WCCT Global has recognized the need to routinely isolate Peripheral Blood Mononuclear Cell (PBMCs) to characterize the human immune response to disease and various immune-targeted therapeutic agents. (wcct.com)
  • Facility
  • WCCT primarily uses the CPT Method for isolating PBMCs during human challenge studies at their Viral Challenge Facility . (wcct.com)
  • diabetes
  • Lymphocytes from a patient with Addison's disease who had GAD65 autoantibodies without diabetes were immortalised and fused to a mouse/human hybridoma. (epfl.ch)
  • serum
  • The human monoclonals, GAD6 and 3/5 mouse monoclonals inhibited serum autoantibody binding to 125I-labelled GAD65. (epfl.ch)
  • separation
  • This objective is unique in the field of tissue separation, and reflects the challenge of islet isolation to achieve these contrasting goals [ 21 ]. (soc-bdr.org)
  • sequences
  • Segments of the human mdr DNA sequences, designated mdr1 and mdr2, have been cloned. (pnas.org)
  • In the 5' flanking region, there are several blocks of sequences that are similar to the sequences of the 5' flanking region of the human c-Ki-ras2 gene. (pnas.org)
  • techniques
  • Furthermore, techniques for the isolation of lung DC from human parenchymal lung tissue and bronchoalveolar lavage fluid (BAL) are described. (springer.com)
  • With the development of techniques for HSC isolation, cultivation and characterization, dramatic achievement has been made in exploring the physiological and pathological functions of HSCs. (springer.com)
  • analysis
  • Genomic Southern blot analysis suggests that the human haploid genome contains a single Gs alpha gene. (pnas.org)
  • S1 nuclease mapping analysis of human Gs alpha mRNA identified multiple transcriptional initiation sites. (pnas.org)
  • adult
  • Several isolates of a human type-C retrovirus belonging to one group, known as human T-cell leukemia virus (HTLV), have previously been obtained from patients with adult T-cell leukemia or lymphoma. (sciencemag.org)
  • gene
  • The structure of the human Gs alpha gene suggests that four types of Gs alpha mRNAs may be generated from a single Gs alpha gene by alternate use of exon 3 and/or of two 3' splice sites of intron 3, where an unusual splice junction sequence (TG) instead of the consensus (AG) is used. (pnas.org)