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  • assay
  • Immediately after exposure, cells were seeded for the colony forming assay. (frontiersin.org)
  • Analysis of radiation-induced damage shortly after exposure showed that C-ions are more effective than X-rays with respect to cell inactivation or the induction of cytogenetic damage (micronucleus assay) as observed in other cell systems. (frontiersin.org)
  • vitro
  • These data establish baseline conditions for using THBMECs to develop in vitro BBB models for studying leukocyte-endothelial interactions during neuroinflammation. (hindawi.com)
  • CCL3 and CCL5 are produced in the CNS of individuals with several neuroinflammatory diseases including MS. Using confluent THBMEC culture as an in vitro BBB model, anti-CCR1 and anti-CCR5 antibodies completely abrogated CCL5-driven mononuclear cell migration across a cytokine-activated BBB [ 5 ]. (hindawi.com)
  • 1995
  • Tissue engineering and autologous transplant formation: practical approaches with resorbable biomaterials and new cell culture techniques (1995) Sittinger M et al. (naver.com)
  • shear
  • Surfaces co-pre-adsorbed with bFN and RGD-SA showed the highest density of attached cells after 8 min of perfusion and the highest percent retention when subjected to shear flow at 60 dynes/cm2 for 2 min. (duke.edu)
  • ligand
  • ECs express B7h, which is the ligand of the ICOS T cell costimulatory molecule, and is also expressed by many cancer cell lines. (unito.it)
  • Surfaces with no ligand treatment showed the lowest cell attachment and retention under flow in all cases. (duke.edu)
  • substrates
  • The research scope and aim is to understand the behavior of endothelial cells on polymer substrates with different microfabricated patterns, using proton Beam Writing (PBW) technique on Polymethyl methacrylate (PMMA) substrates. (nus.edu.sg)
  • hyperglycemia
  • Glucose (25 mmol/l) induced the translocation of PKC-β2-GFP from the cytosol to the membrane in cells grown to hyperglycemia but not in those grown in normal glucose medium. (diabetesjournals.org)
  • We observed significantly increased radical oxygen species production in cells grown in hyperglycemia medium, and this effect was abolished by metformin. (diabetesjournals.org)
  • dependent
  • While, the cells can be aligned along the ridges compared to those on plain surface, and the average speed in the direction perpendicular to the grooves or ridges on different ridges is dependent on ridge geometry. (nus.edu.sg)
  • formation
  • Tissue-like self-assembly in cocultures of endothelial cells and osteoblasts and the formation of microcapillary-like structures on three-dimensional porous biomaterials. (naver.com)
  • protein
  • Cell attachment was evaluated as a function of cell trypsinization (integrin digestion), surface protein formulation, and cell perfusion rate. (duke.edu)
  • perfusion
  • Oscillating perfusion of cell suspensions through three‐dimensional scaffolds enhances cell seeding efficiency and uniformity D. Wendt et al. (naver.com)
  • growth
  • The improvement of vascularization by implementation of endothelial cells or angiogenic growth factors may represent a key solution for engineering bone constructs of large size. (naver.com)