CDK4InhibitorCDK2CyclinInhibitionVitroE2F1Strongly suggestProteinTumor suppPathwaysMutationsPolymeraseTranscriptionalAmountsMiceGenesFunctionallyPathogenesisEGFRCellularEfficacyRecombinantAntibodyApoptosisRolesMolecularTherapeuticProgenitorDiseasesCancersPhenotypesFindings suggestExperimentalCancerGeneticExpressionInduceResultsRegulationCell linesCellsStudiesFamilyDual
CDK411
- The p16 family (p15, p16, p18 and p19) binds to and inhibits the activities of CDK4 and CDK6. (prospecbio.com)
- p15 INK4b and p16 INK4a are closely related proteins and both act on the Rb-pathway through the inhibition of the proliferation-promoting cyclin-dependent kinases CDK4 and CDK6. (biomedcentral.com)
- Based on a database search, we identified abemaciclib, an FDA-approved Cdk4/Cdk6 inhibitor used for the treatment of metastatic breast cancer, as potent inhibitor of HIPK2, HIPK3, and DYRK1A. (nature.com)
- Remarkably, DYRK1A is inhibited by abemaciclib to the same extent as Cdk4/Cdk6 in vitro, raising the question of whether targeting of DYRK1A contributes to the transcriptional inhibition and therapeutic activity of abemaciclib. (nature.com)
- CDK4 inhibition decreases homologous recombination and increases non-homologous end joining, suggesting that CDK4 may also promote error free DNA repair. (rndsystems.com)
- It is a specific inhibitor of cdk4/cdk6, and a tumor suppressor involved in the pathogenesis of a variety of malignancies. (neobiotechnologies.com)
- Acts as a negative regulator of the proliferation of normal cells by interacting strongly with CDK4 and CDK6. (neobiotechnologies.com)
- Analysis of G(1) regulatory proteins demonstrated that protein levels of CDK2, CDK4, cyclin D1, and cyclin A were decreased in a time-dependent manner, but not those of CDK6 and cyclin E, by EB1089. (drlauda.at)
- miR-34a inhibits CDK2 CDK4 and CDK6 as well as E2F a transcription factor that after release from the retinoblastoma tumor suppressor Rb initiates S-phase entry. (careersfromscience.org)
- Puyol supplied another convincing case of how mouse versions may be used to specifically validate a focus on [76] by displaying that Cdk4 however, not Cdk2 or Cdk6 inhibition is certainly artificial lethal with turned on mutant KRAS and will remove mutant KRAS lung tumours. (biopaqc.com)
- p15INK4B is a member of your INK4 family members of CDK inhibitors, which binds to CDK4 and CDK6 subunits, inactivates their catalytic ac tivity and prevents cyclin D CDK4 six complex formation. (mirnaarray.com)
Inhibitor1
- Here, we evaluated the in vitro cytotoxic activity of the cdk2 inhibitor CVT-313 against several human DLBCL cells. (omecamtivmecarbilactivator.com)
CDK23
- Depletion of endogenous cdk2 by short interfering (si)RNA also resulted in apoptosis in human LY3, LY8 and LY18 DLBCL cells. (omecamtivmecarbilactivator.com)
- Importantly, inhibition of cdk2 with CVT-313 or knockdown of endogenous cdk2 with siRNA resulted in downregulation of the anti-apoptotic factor Myeloid cell leukemia-1 (Mcl-1), suggesting that decreased levels of cellular Mcl-1 contribute to apoptosis. (omecamtivmecarbilactivator.com)
- Taken together, these data suggest that cdk2 activity is necessary for the survival of human DLBCL. (omecamtivmecarbilactivator.com)
Cyclin1
- This finding is of utmost importance since p53 is known to be key for p21 expression.2 The results reported here suggest that depletion of Dicer1 restores p21 expression in p53-mutated cells and that miRNA critically contributes to cell cycle regulation at least in part through intervening with the cyclin/CDK system and its inhibitors. (careersfromscience.org)
Inhibition2
- Knockdown of miR-128a and/or miR-130b significantly impaired proliferation of human MLL-AF4 + cell lines, and inhibition of miR-130a significantly impaired disease initiation by the pediatric MLL-AF4 + SEM ALL cell line in immunodeficient NOD- scid IL2Rgamma null (NSG) mice. (ashpublications.org)
- Crataeva Nurvala - (Lupeol is the active compound in Crateva) - Collectively, these data suggest the multitarget efficacy of Lupeol on beta-catenin-signaling network thus resulting in the inhibition prostate cancer cell proliferation. (compassionateacupuncture.com)
Vitro1
- These results suggest prominent in vitro anti-proliferative and pro-apoptotic effects of ST in MDA-MB-231 cells. (biomedcentral.com)
E2F11
- Finally, we manually assembled a proposed regulation network model of subcutaneous preadipocyte differentiation base on the expression data, and suggested that E2F1 may serve as an important link between the processes of duck subcutaneous preadipocyte proliferation and differentiation. (biomedcentral.com)
Strongly suggest3
- CDKIs are capable of suppressing growth, and several lines of evidence strongly suggest that at least some CDKIs may be tumor suppressor proteins. (prospecbio.com)
- Our data strongly suggest that CDK6, DNMT3A, DNMT3B are targets of miR-29a-3p, while CCNE2 and E2F3 are targets of miR-34b-3p. (oncotarget.com)
- Conclusively our results strongly suggest that withaferin A is a potent anticancer agent as ascertained by its potent Hsp90-client modulating capability. (biomedcentral.com)
Protein5
- The human protein shares 94% and 95% aa sequence identity with the mouse and rat orthologs, respectively. (rndsystems.com)
- To map the mechanisms of aggressiveness we aimed to investigate changes in protein phosphorylation across WHO grades of human meningiomas. (nature.com)
- Results from the molecular dynamics simulations in water show that the trajectories of the protein complexed with ligand WA are stable over a considerably long time period of 4 ns, with the energies of the complex being lowered in comparison to the un-docked association complex, suggesting the thermodynamic stability of WA complexed Hsp90/Cdc37. (biomedcentral.com)
- Studies of human genome demonstrate that protein-coding genes only occupy less than 2% of the entire genome [ 1 ]. (ijbs.com)
- 3 Detection of the MLL-AF4 fusion protein in CD34 + /CD19 − fetal hematopoietic stem or progenitor cells or in fetal cells before hematologic specification suggests that there is a window of opportunity during development for the MLL-AF4 fusion protein to immortalize hematopoietic stem and progenitor cells (HSPCs). (ashpublications.org)
Tumor supp1
- Recently, reduced expression of CDK10 has been observed in several cancerous human tissue, suggesting that CDK10 is a tumor suppressor gene. (jcancer.org)
Pathways3
- As the majority of the genes are conserved among mammals, the use of inbred animal model systems may serve as a tool for identification of underlying genes and pathways involved in tumorigenesis in humans. (biomedcentral.com)
- Eventually, Microcystin-LR price the overrepresentation of EGF receptor signal ing pathways from EGFR and ERBB2 three suggests signaling from M7 to M6. (hdac-inhibitors.com)
- Experiments in which components of the TGFβ and insulin/IGF pathways were expressed from tissue-specific promoters suggest that these pathways function predominantly or entirely in the nervous system to control dauer formation [ 8 - 10 ]. (biomedcentral.com)
Mutations1
- Recent analyses of the p16INK4a gene revealed homozygous deletions, nonsense, missense, or frameshift mutations in several human cancers. (neobiotechnologies.com)
Polymerase1
- Recombinant HIPKs and DYRK1A are auto-activated and phosphorylate the negative elongation factor SPT5, the transcription factor c-Myc, and the C-terminal domain of RNA polymerase II, suggesting a direct function in transcriptional regulation. (nature.com)
Transcriptional1
- 1 MLL-AF4 binding upstream of the miR-128a and miR-130b transcriptional start sites suggested direct regulation. (ashpublications.org)
Amounts1
- Finally, a organized investigation from the plasma proteome of GEMMs of NSCLC and SCLC [80] demonstrated significant amounts of similarity towards the human being disease, suggesting which they may be used to find early recognition markers and therapy response monitoring. (biopaqc.com)
Mice3
- Similar to MLL-AF4 + human ALL, pMIRH-128a - and pMIRH-130b -transplanted mice showed significant leptomeningeal tumor cell infiltration. (ashpublications.org)
- Comparative transcriptomic analysis of tumor cells from diseased mice revealed the expression of several well-known MLL-rearranged leukemia-associated target genes, including Meis1 , Runx1 ( pMIRH-128a mice), HoxA9 , Flt3 ( pMIRH-130b mice), Cdk6 , and Bcl2 . (ashpublications.org)
- Propagation of human being tumours in mice is usually demanding with regards to work and costs. (biopaqc.com)
Genes6
- Based on all these data, we propose that miR-29a-3p, miR-34b-3p, miR-181c-5p and miR-517a-3p are disallowed tumor suppressor genes in neuroblastoma and suggest them as new therapeutic targets in neuroblastoma. (oncotarget.com)
- Many insulin/IGF pathway and other known dauer regulatory genes have changes in expression that suggest strong positive feedback by the TGFβ pathway. (biomedcentral.com)
- Up and down regulation of insulin-like ligands and novel genes with similarity to the extracellular domain of insulin/IGF receptors suggests opposing roles for several members of these large gene families. (biomedcentral.com)
- Unlike in adults, most genes with putative DAF-16 binding sites are upregulated during dauer entry, suggesting that DAF-16 has different activity in dauer versus adult metabolism and aging. (biomedcentral.com)
- overexpression of these genes decreased proliferation or induced apoptosis of human MLL-AF4 + ALL cell lines, respectively. (ashpublications.org)
- In addition, the potential for these genes to perform various cognitive roles during human brain evolutionary processes is discussed. (biomedcentral.com)
Functionally1
- Because of this cell fate determining position within the cell's signalosome p53 is lost mutated or functionally inactivated in most human tumor cells. (careersfromscience.org)
Pathogenesis1
- EC development in BDII rats has similarities in pathogenesis, histopathological and molecular properties to human EC, and thus the inbred BDII strain represents a unique model for analysis of EC tumorigenesis [ 14 ]. (biomedcentral.com)
EGFR1
- Curcumin - Based on our findings, we hypothesize that accurate definition of the EGFR status could improve prognostic stratification and we suggest a possible role for EGFR-directed therapies in PC patients. (compassionateacupuncture.com)
Cellular3
- In this study, we investigated how higher-order chromatin structure modulates differential expression of the human INK4b-ARF-INK4a locus during progenitor cell differentiation, cellular ageing and senescence of cancer cells. (biomedcentral.com)
- Autophagy and cellular senescence share a number of stimuli including telomere shortening, DNA damage, oncogenic stress and oxidative stress, suggesting their intimate relationship. (molcells.org)
- Thus, a better understanding of autophagy process will lead us to not only elucidate the conundrum how autophagy plays dual roles in the regulation of cellular senescence but also helps the development of new therapeutic strategies for many human diseases associated with cellular senescence. (molcells.org)
Efficacy2
- Here, we evaluated the efficacy of ST on three different human cancer cell lines including skin epidermoid carcinoma A431 cells, lung epithelial carcinoma A549 cells and breast adenocarcinoma MDA-MB-231. (biomedcentral.com)
- In OA, while it has been shown to reduce the severity of the disease in the murine model [ 8 , 16 ], the senolytic UBX0101 failed to demonstrate clinical efficacy in humans. (aging-us.com)
Recombinant1
- CDKN2A Human Recombinant produced in E.Coli, it's a single non-glycosylated polypeptide chain containing 156 amino acids, approximately 16.5 kDa. (prospecbio.com)
Antibody1
- Formalin-fixed, paraffin-embedded human cervix stained with P16INK4aRecombinant Rabbit Monoclonal Antibody (CDKN2A/4844R). (neobiotechnologies.com)
Apoptosis1
- In types of human being leukemia, dBET1 induced an instant reduced amount of BRD4 and c-MYC, and triggered apoptosis. (p38-mapk-inhibitors.com)
Roles1
- They regulate 60% of all known human mRNAs and play key roles in cell physiology differentiation and carcinogenesis. (careersfromscience.org)
Molecular1
- Comparative molecular analysis of the identified genetic changes in this tumor model with those reported in the human ECs may provide new insights into underlying genetic changes involved in EC development and tumorigenesis. (biomedcentral.com)
Therapeutic1
- Our data suggested that EIF5B is a promising prognostic biomarker and therapeutic target in HCC. (techscience.com)
Progenitor1
- Developmentally regulated EZH2 levels are one of the factors that can determine the higher order chromatin structure and expression pattern of the INK4b-ARF-INK4a locus, coupling human progenitor cell differentiation to proliferation control. (biomedcentral.com)
Diseases3
- In general, genetic studies of complex diseases in human is proven to be difficult due to heterogeneity of the human population with respect to genetic background and diversity of the influencing environmental factors [ 7 - 9 ]. (biomedcentral.com)
- CircRNAs exhibit altered expression under pathological conditions and are strongly associated with the development of various human diseases. (ijbs.com)
- These forms scavenge reactive nitrogen species, inhibit cyclooxygenase- and 5-lipoxygenase-catalyzed eicosanoids and suppress proinflammatory signalling, such as NF-κB and STAT. The animal and human studies show tocotrienols may be useful against inflammation-associated diseases. (biomedcentral.com)
Cancers1
- Altered expression of this gene has been observed in multiple human cancers. (cancerindex.org)
Phenotypes1
- Results and Discussion To study the impact and extent to which miRNA contributes to cell cycle progression in transformed phenotypes we here employed the p53-mutated human keratinocyte cell line (HACAT) and RNA interference (siRNA) techniques. (careersfromscience.org)
Findings suggest2
- Our findings suggest that PTTG3P, a valuable marker of HCC prognosis, promotes tumor growth and metastasis via up-regulating PTTG1 and activating PI3K/AKT signaling in HCC and might represent a potential target for gene-based therapy. (biomedcentral.com)
- These preliminary findings suggest that the initiation of oocyte growth, which seems to lead follicle growth, is associated with diminished p16 expression in the mouse ovary. (biomedcentral.com)
Experimental3
- The non-random nature of these events, as documented by their high frequencies of incidence, is suggesting for dynamic selection of these changes during experimental EC tumorigenesis and therefore for their potential contribution into development of this malignancy. (biomedcentral.com)
- Hence, effort has been made to compile the different functions and properties of tocotrienols in experimental model systems and humans. (biomedcentral.com)
- Considering the wide range of benefits that tocotrienols possesses against some common human ailments and having a promising potential, the experimental analysis accounts for about a small fraction of all vitamin E research. (biomedcentral.com)
Cancer8
- A large number of studies have suggested a role for the INK4b-ARF-INK4a locus in cancer suppression and promotion of ageing. (biomedcentral.com)
- Some modeling analyses suggest 5 separate events are required in colon cancer. (medscape.com)
- These data suggest that EIF5B functions as an oncogene that promotes cancer cell growth, survival, and progression. (techscience.com)
- We suggest that Lupeol could be developed as an agent for chemoprevention as well as chemotherapy of human prostate cancer. (compassionateacupuncture.com)
- Our results suggest that curcumin is an interesting chemopreventive agent for early stage prostate cancer. (compassionateacupuncture.com)
- Our data suggest that reduced CDK10 expression independently predicts a poor prognosis in patients with gastric cancer. (jcancer.org)
- Recently, a series of studies revealed the diagnostic and prognostic value of CDKs (mainly CDK1-7) in different types of human cancer, including oral cancer, medulloblastoma, pancreatic cancer ( 11 - 14 ). (jcancer.org)
- Some have single gene fusion events, possibly targetable, while others have the highest mutation burden in human cancer, but may be excellent candidates for immune therapies. (plos.org)
Genetic1
- The small number of potentially cooperating genetic lesions suggests that MLL-AF4 is sufficient to induce infant ALL. (ashpublications.org)
Expression2
- Expression of p16INK4a (p16 positive) is highly correlated with human papilloma virus (HPV) infection in head and neck squamous cell carcinomas (HNSCC). (neobiotechnologies.com)
- On the other hand, etoposide treatment reliably induces DNA damage-related senescence in human articular chondrocytes evidenced by loss of proliferative capacity, DNA damage accumulation, and expression of some SASP components. (aging-us.com)
Induce1
- This study used etoposide, to induce DNA damage-related senescence or chronic exposure to IL-1β to entail inflammation-related senescence in human OA chondrocytes. (aging-us.com)
Results1
- Future trials should display whether these 668270-12-0 supplier results could be translated to human being lung malignancy. (biopaqc.com)
Regulation1
- However, our observation that the position of putative DAF-16 binding sites is correlated with the direction of regulation suggests a novel method of achieving gene-specific regulation from a single pathway. (biomedcentral.com)
Cell lines2
- In the present study, we analyzed the effect of EB1089 on human myeloma cell lines. (drlauda.at)
- Right here, establishing an adequate collection of human being examples and cell lines usually takes years, while a mutant ALK GEMM could be exploited like a much faster option to gain understanding into the root signalling systems and check treatment regimens. (biopaqc.com)
Cells1
- This suggests that the very high affinity of HIV-1 RT for dNTPs prevents HIV-1 restriction by SAMHD1 in cycling cells. (biomedcentral.com)
Studies1
- Cytogenetic studies of human ECs have not produced very conclusive data, since many of these studies are based on karyotyping of limited number of cases and no really specific karyotypic changes have yet been identified. (biomedcentral.com)
Family1
- The DYRK family has been characterized in organisms from yeast to humans and can be divided into five subfamilies, class I DYRKs, class II DYRKs, YAKs, HIPKs, and PRP4s 1 . (nature.com)
Dual1
- Here, we examine growth suppression efficiency of human glioblastomas by dual-peptide targeting. (aacrjournals.org)