• Derivation and differentiation of haploid human embryonic stem cells. (nature.com)
  • After development of the optic vesicle from the induced stem cell embryoid body in three-dimensional culture, conversion to two-dimensional culture, achieved by supplementation with BDNF, resulted in differentiation of RGCs at a rate of nearly 90% as indicated by a marginal subregion of an extruded clump of cells, suggesting the formation of an optic vesicle. (nih.gov)
  • Differentiation of human and murine induced pluripotent stem cells to microglia-like cells. (alzforum.org)
  • Lastly, differentiation of iPSCs into trophoblast cells revealed that errors in reprogramming CG methylation are transmitted at a high frequency, providing an iPSC reprogramming signature that is maintained after differentiation. (ca.gov)
  • To address this issue, we sub-cloned candidate human iPSC lines and deeply characterized their genetic properties using whole genome sequencing, their genomic stability upon CRISPR-Cas9-based gene editing, and their phenotypic properties including differentiation to commonly used cell types. (ca.gov)
  • In this webinar, Dr. James Wells discusses the generation of human intestinal organoids containing a functional enteric nervous system, the differentiation of gastric organoids, and the application of these systems to examine intestinal motility disorders and gastric infection with Helicobacter pylori. (stemcell.com)
  • Here, we employed a human pancreatic differentiation platform complemented with an shRNA screen in human pluripotent stem cells (PSCs) to identify potential drivers of early endoderm and pancreatic development. (mdpi.com)
  • Protocols for hepatocyte differentiation from iPS cells have been studied. (spandidos-publications.com)
  • It was hypothesized that the addition of glucose and galactose promoted hepatocyte differentiation of iPS cell. (spandidos-publications.com)
  • To address these questions, particularly in a human-relevant system, we have focused upon the use of human induced pluripotent stem cells (iPSCs) to assess their ability to give rise to RGCs, with a particular emphasis upon ipRGC differentiation. (indianactsi.org)
  • By immunostaining for cell type specific markers, I have documented the different stages of retinal differentiation and provided insight into the mechanisms of ipRGC differentiation. (indianactsi.org)
  • Using this novel differentiation strategy we have demonstrated that lab-grown dopamine nerve cells can efficiently engraft and function in mouse, rat or monkey models of PD. (sloankettering.edu)
  • Nkx2.1 progenitor cells at 12 days of differentiation from iPSC integrated into the hypothalamus following injection into the lateral ventricle of NSG mice. (columbia.edu)
  • Unguided differentiation confirms HiPSC ability to generate cell derivatives of tissues arising from the three embryonic layers. (cellapplications.com)
  • Our complete portfolio of stem cell research products and services provides optimized tools for each step of the stem cell workflow: from reprogramming somatic cells to iPSC expansion, validation, and differentiation. (thermofisher.com)
  • Gibco media, supplements, substrates, growth factors and cytokines provide you with an easy-to-use, flexible set of tools for targeted differentiation of induced pluripotent stem cells. (thermofisher.com)
  • Our differentiation portfolio allows optimal growth, expansion and storage of differentiated cells-enabling faster, more efficient systems. (thermofisher.com)
  • Abstract Background Cardiac differentiation of human-induced pluripotent stem (hiPS) cells consistently produces a mixed population of cardiomyocytes and non-cardiac cell types, even when using well-characterized protocols. (figshare.com)
  • We sought to determine whether different cell types might result from intrinsic differences in hiPS cells prior to the onset of differentiation. (figshare.com)
  • Additionally, we found that substantial cell death occurs during differentiation in a manner that suggested all cells were equally likely to survive or die, suggesting that there is no intrinsic selection bias for cells descended from particular hiPS cell progenitors. (figshare.com)
  • We thus wondered how cells grow spatially during differentiation, so we labeled cells by expression of marker genes and found that cells expressing the same marker tended to occur in patches. (figshare.com)
  • instead, factors specifying the various cell types likely act during a window that begins shortly after the seeding of hiPS cells for differentiation. (figshare.com)
  • Cardiac in vitro models have become increasingly obtainable and affordable with the optimization of human pluripotent stem cell-derived cardiomyocyte (hPSC-CM) differentiation. (mssm.edu)
  • Australia allowed the other download Human Embryonic and Induced Pluripotent Stem Cells: Lineage Specific Differentiation Protocols to impose software for methods in 1902 and Canada was also system centuries as emotional Goodreads by video until 1922. (idealnaja.pl)
  • This change in gene activity blocks the maturation (differentiation) of blood cells and leads to the production of abnormal, immature white blood cells called myeloid blasts. (medlineplus.gov)
  • Stem and progenitor cell populations are often heterogeneous, which may reflect stem cell subsets that express subtly different properties, including different propensities for lineage selection upon differentiation, yet remain able to interconvert. (lu.se)
  • A key challenge is to understand how state, but must also afford flexibility in cell-fate choice to permit the different cell-fate options confronting stem and progenitor cell-type diversification and differentiation in response to cells are selected and coordinated such that adoption of a given intrinsic cues or extrinsic signals. (lu.se)
  • Evidence the fate of stem cells has broad ramifications for biomedical suggests that during development or differentiation, cells make science from elucidating the causes of cancer to the use of very precise transitions between apparently stable ``network stem cells in regenerative medicine. (lu.se)
  • Many existing protocols for neuronal differentiation of human pluripotent cells result in heterogeneous cell populations and unsynchronized differentiation, necessitating the development of methods for labeling specific cell populations. (lu.se)
  • We provide detailed protocols for lentiviral transduction, neural differentiation, and subsequent analysis of human embryonic stem cells. (lu.se)
  • Human induced pluripotent stem cells (hiPSCs) and human embryonic stem cells (hESCs) are promising candidate cell sources for regenerative medicine. (jci.org)
  • However, despite the common ability of hiPSCs and hESCs to differentiate into all 3 germ layers, their functional equivalence at the single cell level remains to be demonstrated. (jci.org)
  • Here, we used single cell analysis to resolve the gene expression profiles of 362 hiPSCs and hESCs for an array of 42 genes that characterize the pluripotent and differentiated states. (jci.org)
  • Comparison between single hESCs and single hiPSCs revealed markedly more heterogeneity in gene expression levels in the hiPSCs, suggesting that hiPSCs occupy an alternate, less stable pluripotent state. (jci.org)
  • Our results suggest that caution should be exercised before assuming that hiPSCs occupy a pluripotent state equivalent to that of hESCs, particularly when producing differentiated cells for regenerative medicine aims. (jci.org)
  • While human induced pluripotent stem cells (hiPSCs) provide novel prospects for disease-modeling, the high phenotypic variability seen across different lines demands usage of large hiPSC cohorts to decipher the impact of individual genetic variants. (frontiersin.org)
  • Thus, a much higher grade of parallelization, and throughput in the production of hiPSCs is needed, which can only be achieved by implementing automated solutions for cell reprogramming, and hiPSC expansion. (frontiersin.org)
  • We have developed a feeder-free, Sendai virus-mediated reprogramming protocol suitable for cell culture processing via a robotic liquid handling unit that delivers footprint-free hiPSCs within 3 weeks with state-of-the-art efficiencies. (frontiersin.org)
  • Automatically expanded hiPSCs exhibit normal growth characteristics, and show sustained expression of the pluripotency associated stem cell marker TRA-1-60 over at least 5 weeks (10 passages). (frontiersin.org)
  • The advent of the human induced pluripotent stem cells (hiPSCs) technology offers unprecedented opportunities for disease modeling, personalized medicine, and the development of new therapeutic interventions. (frontiersin.org)
  • We aim to develop a cell culture platform to automatically differentiate human induced pluripotent stem cells (hiPSCs) for lab-on-a chip applications. (dim-elicit.fr)
  • Our HiPSCs display classic pluripotent stem cell morphology, with a high nucleus to cytoplasm size ratio, as well as they are amenable to be cultivated in serum-free media, independent of feeder cells and of feeder-conditioned media as colonies or high density monolayers. (cellapplications.com)
  • Post-thawing viability of HiPSCs is typically higher than 70%, and HiPSC have demonstrated coherent pluripotent behavior over more than 60 passages. (cellapplications.com)
  • The implementation of human pluripotent stem cells (hiPSCs) in cell therapy has an extraordinary potential but faces many practical challenges, including costs associated with growth media and factors. (authorea.com)
  • Given this, human pluripotent stem cells, such as human embryonic stem cells (hESCs) and induced pluripo- tent stem cells (hiPSCs), have become a unique cell source for study- ing early human brain development. (lu.se)
  • Finding physiologically relevant, reproducible, and reliable cell models that can be used to detect these end points at preclinical and discovery stages has been a challenge, as most toxicity testing is done either by using immortalized cancer cell lines, primary explanted somatic cells, or live animals. (the-scientist.com)
  • Here we report the first whole-genome profiles of DNA methylation at single-base resolution in five human iPSC lines, along with methylomes of ES cells, somatic cells, and differentiated iPSCs and ES cells. (ca.gov)
  • Human induced pluripotent stem (iPS) cells can differentiate into somatic cells ( 1 ). (spandidos-publications.com)
  • Reprogramming of somatic cells for iPSC generation can be accomplished with a number of technologies. (thermofisher.com)
  • In reality, gene somatic cells to a pluripotent cell state by a handful of transcrip- expression is graded, making the potential gene expression tion factors (Takahashi and Yamanaka, 2006). (lu.se)
  • She previously worked as a postdoctoral scientist for two years in GlaxoSmithKline's Safety Pharmacology group, where her research focused on developing and validating an electrophysiology assay using human induced pluripotent stem cell-derived cardiomyocytes for cardiac safety screening. (the-scientist.com)
  • Her current research focuses on utilizing cellular impedance measures of human iPSC-derived cardiomyocytes to deconvolute kinase inhibitor cardiotoxicity. (the-scientist.com)
  • Ranolazine rescues the heart failure phenotype of PLN-deficient human pluripotent stem cell-derived cardiomyocytes. (stembook.org)
  • Cardiomyocytes (CMs) derived from human pluripotent stem cells (hPSCs) provide a valuable cell source for regenerative therapy, disease modeling, and drug screening. (biomedcentral.com)
  • In this study, we demonstrated an approach using human induced pluripotent stem cellā€derived atrial and ventricular cardiomyocytes (hiPSCā€aCMs and hiPSCā€vCMs, respectively) coupled with a sophisticated optical mapping system for drug screening of atrialā€selective compounds in vitro. (sfu.ca)
  • A Mount Sinai-led team has developed a reproducible and scalable method to advance maturation of human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs)-;cells that support heart muscle contraction, generated in the lab from human stem cell lines-;which researchers say will improve approaches for disease modeling, regenerative therapies, and drug testing. (azolifesciences.com)
  • 2022) PPARdelta activation induces metabolic and contractile maturation of human pluripotent stem cell-derived cardiomyocytes. (azolifesciences.com)
  • Hepatocytes derived from iPS cells are suitable for transplantation into patients with liver insufficiency and for use in toxicity tests ( 2 ). (spandidos-publications.com)
  • Human pluripotent stem cell-derived dopamine neurons after transplantation into the brain of a Parkinsonian monkey. (sloankettering.edu)
  • Subsequent transplantation of the cells into lethally irradiated mice results in engraftment of the animals with donor haematopoietic tissue containing the bacterial gene. (nature.com)
  • Functional CMs derived from human pluripotent stem cells (hPSCs) would provide an unprecedented cell source for disease modeling, drug discovery, and cell transplantation therapy for CVD patients [ 3 ]. (biomedcentral.com)
  • The idea is that these will be analogous to the O-negative "universal-donor" blood type and could be employed for all cell-based transplantation therapies in patients with immune rejection, said Dr Cowan. (medscape.com)
  • The clinical team from Lund headed by Prof. Olle Lindval in collaboration with Prof. Anders Bjƶrklund, were the first to show in patients the proof of principle that cell transplantation therapy is feasible approach to treat the patients with Parkinson's disease. (lu.se)
  • Accumulated data indicate that hematopoietic stem cell transplantation may be effective under optimal conditions in preventing the progression of central nervous system symptoms in neuronopathic forms of lysosomal storage diseases (such as Krabbe disease), including some of the mucopolysaccharidoses, oligosaccharidoses, sphingolipidoses, and lipidoses as well as peroxisome disorders such as X-linked adrenoleukodystrophy. (medscape.com)
  • Although longitudinal natural history data are limited, published guidelines are available to assist with decisions related to the pursuit of transplantation and whether to use bone marrow or umbilical cord blood-derived cells. (medscape.com)
  • Some evidence indicates that at least in certain disorders, combination ERT and hematopoietic stem cell transplantation together might be superior to hematopoietic stem cell transplantation alone in patients who are appropriate candidates. (medscape.com)
  • The availability of both ERT and hematopoietic stem cell transplantation has prompted ongoing consideration of newborn screening efforts to diagnose lysosomal storage diseases. (medscape.com)
  • Stem cell transplantation is the removal of stem cells (undifferentiated cells) from a healthy person and their injection into someone who has a serious blood disorder. (msdmanuals.com)
  • Overview of Transplantation Transplantation is the removal of living, functioning cells, tissues, or organs from the body and then their transfer back into the same body or into a different body. (msdmanuals.com)
  • But researchers think that these stem cells have the most potential for producing different kinds of cells and for surviving after transplantation. (msdmanuals.com)
  • These stem cells are most often used for transplantation. (msdmanuals.com)
  • Neuronal cells expressing pro-opiomelanocortin (POMC), neuropeptide-Y/agouti-related protein (NPY/AgRP) were generated from human embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) obtained from patients with monogenic forms of obesity. (columbia.edu)
  • Importantly, these cells displayed hypothalamic neuronal characteristics, including production and secretion of neuropeptides and responsiveness to metabolic hormones such as insulin and leptin. (columbia.edu)
  • Video: Human iPSC-Derived Neurons establish mature, synchronized neuronal network. (cellapplications.com)
  • HiPSC differentiate into cell derivatives from the 3 embryonic layers: Neuronal marker beta III tubulin (TUJ1), Smooth Muscle Actin (SMA) and Hepatocyte Nuclear Factor 3 Beta (HNF3b). (cellapplications.com)
  • For gene therapy, a combinatorial approach with multiple target genes, as well as more complex optogenetic approaches are studied, while for cell-therapy human induced pluripotent stem cells and human induced neuronal cells are investigated. (lu.se)
  • Organoids are three-dimensional (3D) cell cultures containing a self-renewing stem cell population which differentiates into multiple, organ-specific cell types demonstrating key features of the represented organ. (stemcell.com)
  • Human liver organoids are formed from the assembly of hepatocyte-like cells differentiated from iPS cells, human umbilical vascular endothelial cells and mesenchymal stem cells ( 9 ). (spandidos-publications.com)
  • Human liver organoids are expected to be used as a 'mini-liver' instead of resected liver fragments ( 10 ). (spandidos-publications.com)
  • We found that iPSCs can be effectively differentiated into retinal cells, including the formation of 3D retinal organoids. (indianactsi.org)
  • Scientists working to bioengineer the entire human gastrointestinal system in a laboratory now report using pluripotent stem cells to grow human oesophagal organoids. (drugtargetreview.com)
  • Researchers report in the journal Cell Stem Cell the organoids enhance the study of oesophagal disorders, personalised medicine and the development of regenerative tissue therapies for people. (drugtargetreview.com)
  • The scientists based their new method for using human PSCs to general oesophagal organoids on precisely timed, step-by-step manipulations of genetic and biochemical signals that pattern and form embryonic endoderm and foregut tissues. (drugtargetreview.com)
  • After successfully generating fully formed human oesophagal organoids-which grew to a length of about 300-800 micrometres in about two months-the bioengineered tissues were compared biochemically to oesophagal tissues from patient biopsies. (drugtargetreview.com)
  • We developed a model of the aberrant epithelial reprogramming observed in IPF using alveolar organoids derived from human-induced pluripotent stem cells stimulated with a cocktail of pro-fibrotic and inflammatory cytokines. (lu.se)
  • Deconvolution of RNA-seq data of alveolar organoids indicated that the fibrosis cocktail rapidly increased the proportion of transitional cell types including the KRT5-/KRT17+ aberrant basaloid phenotype recently identified in the lungs of. (lu.se)
  • Sozzi E, Nilsson F, Kajtez J, Parmar M, Fiorenzano A. Generation of Human Ventral Midbrain Organoids Derived from Pluripotent Stem Cells . (lu.se)
  • To exploit the full potential of human pluripotent stem cells for regenerative medicine, developmental biology and drug discovery, defined culture conditions are needed. (nih.gov)
  • Cells from the blood samples are reprogrammed into clinical-grade human induced pluripotent stem cells, which can be distributed to recipients for regenerative medicine. (wjgnet.com)
  • Induced pluripotent stem cells (iPSCs) offer immense potential for regenerative medicine and studies of disease and development. (ca.gov)
  • They may also serve as an unlimited source of therapeutically active cells for regenerative medi- cine. (lu.se)
  • You can put islet cells into someone with type 1 diabetes and they can leave the hospital 3 days later with no diabetesā€¦.But you're not going to get very far with human donors," press briefing moderator Gordon C Weir, MD, professor of medicine at Harvard Medical School, and cohead of the Joslin Diabetes Center's section on islet cell and regenerative biology, told Medscape Medical News . (medscape.com)
  • If successful, this proposed work could have an enormous impact on regenerative medicine, leading the way to rigorously tested universal-donor stem cells that could be grown and differentiated into very large numbers of cells, made widely available to all medical institutions and used on demand to treat patients with type 1 diabetes and a variety of degenerative diseases, he concluded. (medscape.com)
  • Using our recently established haploid human pluripotent stem cells (hPSCs), we generated a genome-wide loss-of-function library targeting 18,166 protein-coding genes to define the essential genes in hPSCs. (nature.com)
  • Overall, we have constructed an atlas of essential and growth-restricting genes in hPSCs, revealing key aspects of cellular essentiality and providing a reference for future studies on human pluripotency. (nature.com)
  • Development of reliable methods to culture complex retinal tissues from human pluripotent stem cells (hPSCs) could offer a means to study human retinal development, provide a platform to investigate the mechanisms of retinal degeneration and screen for neuroprotective compounds, and provide the basis for cell-based therapeutic strategies. (utah.edu)
  • My thesis research focused on establishing an in vitro model for understanding the molecular neurophysiology of obesity using, as 'proof-of-principle', neurons derived from human pluripotent stem cells (hPSCs) derived from individuals with monogenic forms of obesity. (columbia.edu)
  • This project was designed to establish an in vitro model for studying hypothalamic cell-molecular physiology in neurons derived from hPSCs. (columbia.edu)
  • reported a protocol to selectively differentiate hPSCs into CMs with nodal-type characteristics, which relies on co-culture with visceral endoderm-like cells and subsequent culture in a serum-enriched medium [ 12 ]. (biomedcentral.com)
  • Here, we developed an in vitro model of human ectodermal patterning, in which human pluripotent stem cells (hPSCs) self -organize to form a radially regionalized neural and non- central nervous system (CNS) ectoderm . (bvsalud.org)
  • Human pluripotent stem cells, with their ability to proliferate indefinitely and to differentiate into virtually all cell types of the human body, provide a novel resource to study human development and to implement relevant disease models. (mdpi.com)
  • Under these conditions, iPS cells begin to differentiate into a hepatocyte lineage. (spandidos-publications.com)
  • Its mandate was to advise CIHR as to whether human embryonic stem cell and human embryonic germ cell research should be considered eligible for CIHR funding. (gc.ca)
  • The Act applies to the derivation of human pluripotent stem cells from human embryos, but does not apply to research using human embryonic stem cell lines that have already been derived. (gc.ca)
  • Here, we have generated a CHMP2B-mutated human embryonic stem cell line using genome editing with the purpose to create a human in vitro FTD disease model. (lu.se)
  • We discuss these properties with examples both from the hematopoietic and embryonic stem cell (ESC) systems. (lu.se)
  • Retinal ganglion cells can be purified and matured in vitro to generate models for optic neuropathies. (indianactsi.org)
  • however, cell sorting and their segregation in specific germ layer combinations have not been investigated yet in a human -specific in vitro system. (bvsalud.org)
  • Human induced pluripotent stem cell-derived mesenchymal stem cells (iPS-MSCs) are emerging as attractive options for use in cell replacement therapy, but their effect in kidney diseases remains unknown. (oncotarget.com)
  • We report thermo-responsive retinoic acid (RA)-loaded poly(N-isopropylacrylamide)-co-acrylamide (PNIPAM-co-Am) nanoparticles for directing human induced pluripotent stem cell (hiPSC) fate. (edu.au)
  • Somatic cell reprogramming involves epigenomic reconfiguration, conferring iPSCs with characteristics similar to embryonic stem (ES) cells. (ca.gov)
  • Our products have been an integral part of induced pluripotent stem cell research from the initial discovery of iPSCs to current breakthroughs. (thermofisher.com)
  • Here, we report the generation and characterization of induced pluripotent stem cells (iPSCs) derived from peripheral blood mononuclear cells of three individuals above 70 years of age without AMD. (ru.nl)
  • We are particularly focused on improving the iPSC-based tools for modeling late-onset disorders such as Parkinson's disease using state-of-the-art genetic tools for gene repair, cell purification, and manipulation of cell maturation and age. (mskcc.org)
  • On the strength of these findings, we have made KOLF2.1J and its gene-edited derivative clones readily accessible to promote the standardization required for large-scale collaborative science in the stem cell field. (ca.gov)
  • Cells were initially edited by the CRISPR/Cas9 system to express the Thy1 gene at the Brn3b locus and the tdTomato gene at the melanopsin locus. (indianactsi.org)
  • Choose from Invitrogen gene editing and transfection technologies to build cell models that can better answer biological questions. (thermofisher.com)
  • An infectious retrovirus vector has been used to transfer a bacterial gene encoding resistance to the neomycin analogue G418 into pluripotent haematopoietic stem cells present in explanted murine bone marrow tissue. (nature.com)
  • The scientists report that during critical stages of embryonic development, the Sox2 gene blocks the programming and action of genetic pathways that direct cells to become respiratory instead of oesophagal. (drugtargetreview.com)
  • Here we study the cellular phenotype of hPSC-CMs by comparing their single-cell gene expression and functional profiles in three engineered cardiac tissue configurations: human ventricular (hv) cardiac anisotropic sheet, cardiac tissue strip, and cardiac organoid chamber (hvCOC), with spontaneously aggregated 3D cardiac spheroids (CS) as control. (mssm.edu)
  • While the signaling effects of the protein peroxisome proliferator activated receptor alpha (PPARa) are the most active in heart muscle cells, the researchers said PPARd signaling has a separate and important role in efficiently activating the gene regulatory networks, increasing the quantity and organization of the organelles involved in energy production, and augmenting the fatty acid oxidation process. (azolifesciences.com)
  • Similarly, GATA-1 has been shown to induce lineage switching expression values even if, for simplicity, we assume only ``on'' of committed cells in hematopoiesis, first in cell lines (Kulessa and ``off'' states for each gene. (lu.se)
  • Immunogenicity is reduced by removing the major histocompatibility complex molecules from the stem cells with gene editing, and tolerance induced via "immune editing. (medscape.com)
  • Main emphasis of Epilepsy Center is to explore mechanisms underlying epileptogenesis, and develop gene- and cell-therapy based treatment strategies for epilepsy. (lu.se)
  • 2. Nuclear transfer is a technique used to duplicate genetic material by creating an embryo through the transfer and fusion of a diploid cell in an enucleated female oocyte.2 Cloning has a broader meaning than nuclear transfer as it also involves gene replication and natural or induced embryo splitting (see Annex 1). (who.int)
  • 2008). Historically, this concept is highlighted by the experi- factors are key intrinsic regulators of these fate decisions and mental phenomenon of lineage reprogramming, for example, that fate choice involves modulating networks of transcription by the conversion of fibroblasts to muscles cells following trans- factors. (lu.se)
  • The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
  • In this investigation we sought to answer the question concerning the presence of mast cells in human and rat dental pulp tissues, under normal and inflammatory conditions. (bvsalud.org)
  • Researchers hope to use stem cells to repair or replace cells or tissues damaged or destroyed by such disorders as Parkinson disease, diabetes, and spinal injuries. (msdmanuals.com)
  • doi: 10.1016/j.stem.2016.10.015. (wisc.edu)
  • However, it remains unknown how complete the reestablishment of ES-cell-like DNA methylation patterns is throughout the genome. (ca.gov)
  • Since the virus does not go through a DNA phase, its genetic material and transgenes do not integrate into the host cell genome. (cellapplications.com)
  • The human genome at one time was estimated to have 70,000-100,000 genes. (medscape.com)
  • New data from the Human Genome Project suggest this number may be closer to 30,000. (medscape.com)
  • Data from the Human Genome Project surely will be useful in identifying mutations in the thousands of genes that must underlie inherited diseases of the central and peripheral nervous system. (medscape.com)
  • explosion further, consider that a fictitious small genome with 2002) More recently and more dramatically, the potential for 260 genes would host the same number of combinations as cell state conversions is exemplified by the reprogramming of the number of atoms in the visible universe! (lu.se)
  • hPSC-enriched essential genes mainly encode transcription factors and proteins related to cell-cycle and DNA-repair, revealing that a quarter of the nuclear factors are essential for normal growth. (nature.com)
  • Fig. 2: Analysis of cell-essential genes. (nature.com)
  • The scientists used mice, frogs and human tissue cultures to identify other genes and molecular pathways regulated by Sox2 during oesophagus formation. (drugtargetreview.com)
  • The RUNX1 protein turns on (activates) genes that help control the development of blood cells (hematopoiesis). (medlineplus.gov)
  • While t(8;21) is important for leukemia development, a mutation in one or more additional genes is typically needed for the myeloid blasts to develop into cancerous leukemia cells. (medlineplus.gov)
  • By triggering certain genes, researchers may be able to cause the stem cells to specialize and become the cells that need to be replaced. (msdmanuals.com)
  • One way to induce these cells is to inject them with material that affects their genes, a process called reprogramming. (msdmanuals.com)
  • Cincinnati Children's scientists and their multi-institutional collaborators already have used PSCs to bioengineer human intestine, stomach, colon and liver. (drugtargetreview.com)
  • Here, we describe the StemCellFactory, an automated, modular platform covering the entire process of hiPSC production, ranging from adult human fibroblast expansion, Sendai virus-based reprogramming to automated isolation, and parallel expansion of hiPSC clones. (frontiersin.org)
  • HiPSC-derived Neural Stem Cells (L) and Neurons (R) . i-HNSC stained w/ Nestin (neural stem cell marker, green), SOX 2 (stem cell marker, red) & DAPI (nuclear stain, blue). (cellapplications.com)
  • These hypothalamic-like neurons accounted for over 90% of differentiated cells and exhibited transcriptional profiles characteristic of specific hypothalamic neurons (and explicitly lacking pituitary markers). (columbia.edu)
  • However, when the same hiPS cells were differentiated in parallel, we did not observe high transcriptional similarity across differentiations. (figshare.com)
  • In terminally differentiated cell fate is coupled to appropriate regulation of the alternative cells, transcriptional networks must be stable and irreversible, pathways. (lu.se)
  • The goal of the Initiative is to generate and characterize a large number of human iPSC lines as a resource for the global research community. (genengnews.com)
  • We are interested in applying our ability to generate disease-relevant cell types and in establishing clinical-grade protocols for the development of cell therapy approaches in other CNS and PNS disorders. (sloankettering.edu)
  • This requires a more precise understanding of the genetic and biochemical mechanisms behind their cause-a need filled by the ability to generate and study robust, functional, genetically matched models of human oesophagal tissue that can be grown from a person's own cells. (drugtargetreview.com)
  • The Keller group described a transgene-independent method to generate sinoatrial node (SAN)-like pacemaker cells, which were identified as NKX2-5-negative CMs expressing SAN markers and displaying expected electrophysiological properties [ 13 ]. (biomedcentral.com)
  • They found that PPARd induces the metabolic switch from glycolysis to fatty acid oxidation in the lab setting, thus influencing whether heart muscle cells generate energy from glucose or fatty acids. (azolifesciences.com)
  • NSCs are derived from the embryonic stem (ES) cells or fetal and adult nervous system, and can generate different type of neural cells. (lu.se)
  • The Scientist brings together a panel of experts to educate you about the successes and caveats of using iPSC-derived cells in toxicological assays. (the-scientist.com)
  • Reprogramming triggers a cascade of evident changes in the host cells that are recognizable morphologically and through a combination of markers and pluripotency assays. (cellapplications.com)
  • The ability to induce age-like features in PD-iPSC-derived dopamine nerve cells has yielded late-stage disease phenotypes not commonly observed in standard PD-iPSC models. (mskcc.org)
  • In this study we describe the generation and basic functional characteristics of mDA neurons derived from a human pluripotent stem cell (hPSC) line expressing eGFP under the control of the PITX3 promoter. (monash.edu)
  • Ion-channel block can lead to acute and delayed arrhythmias, while biochemical toxicities can result in cell death, abnormal cardiac function, and even heart failure and death. (the-scientist.com)
  • Current drug development efforts for the treatment of atrial fibrillation are hampered by the fact that many preclinical models have been unsuccessful in reproducing human cardiac physiology and its response to medications. (sfu.ca)
  • At Axion BioSystems, our goal is to help you capture the complex biology of your cells. (axionbiosystems.com)
  • This work will create exciting opportunities to further assess human heart biology through multi-disciplinary approaches incorporating developmental biology, transcriptomics, contractile measurements and drug testing. (azolifesciences.com)
  • Understanding cell-fate decisions in stem cell populations is a major goal of modern biology. (lu.se)
  • Lund center for Stem Cell Biology and Cell Therapy is one of six Swedish strategic centers of excellence in life sciences, supported by the Swedish Foundation for Strategic Research. (lu.se)
  • In January 2002, after a year of discussion and consultation, the group produced a report to CIHR's Governing Council, which was unanimously accepted and formed the basis of human pluripotent stem cell research guidelines that were publicly announced in March 2002. (gc.ca)
  • The former CIHR Guidelines for Human Pluripotent Stem Cell Research (2002, 2005, 2006, 2007, and 2010), the Report of the ad hoc working group (2002) and Discussion paper (2001) are available in electronic format on request. (gc.ca)
  • The symposium was funded by the Royal Physiographic Society and its partners at Lund University Cancer Center, Lund Stem Therapy, Lund Stem Cell Center and the Geological Department. (lu.se)
  • Welcome to stemcellcenter.lu.se, which is managed by the Lund Stem Cell Center at Lund University. (lu.se)
  • The activation of signaling regulated by PPARd can further enhance heart muscle cell size and organization, and improve contractility, all hallmarks of heart maturation. (azolifesciences.com)
  • Human induced pluripotent stem cell (iPSC) lines are a powerful tool for studying development and disease, but the considerable phenotypic variation between lines makes it challenging to replicate key findings and integrate data across research groups. (ca.gov)
  • Stem cell research has the potential to provide treatments for a host of debilitating diseases including Alzheimer's, Parkinson's, diabetes, multiple sclerosis, heart disease, and spinal cord injury. (gc.ca)
  • In recognition of this and because of the complex ethical issues that it raises, the President of CIHR convened the Ad Hoc Working Group on Stem Cell Research in the fall of 2000. (gc.ca)
  • Until then, Canada had no laws to govern human pluripotent stem cell research, nor were there any guidelines for researchers, research ethics boards, or funding agencies on how human pluripotent stem cells may be derived and used. (gc.ca)
  • The guidelines provided for the review of human stem cell research applications by a Stem Cell Oversight Committee (SCOC). (gc.ca)
  • In March 2004, an Act Respecting Assisted Human Reproduction and Related Research , became law. (gc.ca)
  • In 2014, the guidelines were integrated into the 2nd Edition of the Tri-Council Policy Statement: Ethical Conduct for Research Involving Humans (TCPS 2) . (gc.ca)
  • We offer choices in integration-free reprogramming technologies and services that fit your research and translational needs including our Cell Therapy Systems (CTS) Products, which are designed for clinical and translational research applications. (thermofisher.com)
  • From feeder-based and feeder-free media to optimized passaging reagents and substrates to media for stem cell therapy research, Gibco products enable you to culture with confidence. (thermofisher.com)
  • Analyze stem cells and their derivatives using a wide range of cellular and molecular characterization methods, as validation is critical in iPSC research. (thermofisher.com)
  • The European Bank for induced pluripotent Stem Cells performs collection, banking, quality control and distribution of iPSC lines for research purposes. (wikipedia.org)
  • We offer a new course containing substantial hand-on practice in the cell lab as well as seminars covering topics such as history, growth, quality controls and how they can be used in research and the clinic. (lu.se)
  • The aim of this 5-day course is to get a better theoretical and practical understanding of the pluripotent stem cells, their culture requirements and extensive potential for use in modern research and medicine. (lu.se)
  • The research team also identified the role of the protein known as peroxisome proliferator activated receptor delta (PPARd) in inducing what is referred to as the metabolic switch in the lab-generated heart muscle cells. (azolifesciences.com)
  • The research team also investigated the effects of lactate exposure, where heart muscle cells are able to survive on lactate in the absence of glucose. (azolifesciences.com)
  • The team's current research focus is to create "universal-donor" pluripotent stem-cell lines that are induced to grow into insulin-producing beta cells. (medscape.com)
  • Research groups are gathered within five main research clusters: MultiPark, Stem Cell Center and Epilepsy Center. (lu.se)
  • The possibility to isolate and propagate NSCs and their potential applications in cell therapy have attracted a lot of research interest in recent years. (lu.se)
  • 5. In 2001, France and Germany requested the United Nations General Assembly to develop international conventions on human reproductive cloning, therapeutic cloning and research on stem cells. (who.int)
  • Sagi, I. & Benvenisty, N. Haploidy in humans: an evolutionary and developmental perspective. (nature.com)
  • Advancing knowledge of human development, developmental disabilities, and neurodegenerative diseases. (wisc.edu)
  • The human developmental processes during the early post-implantation stage instruct the specification and organization of the lineage progenitors into a body plan. (bvsalud.org)
  • The resulting cells maintained a normal karyotype and had high levels of pluripotency markers. (nih.gov)
  • In particular, it plays an important role in development of hematopoietic stem cells, early blood cells that have the potential to develop into all types of mature blood cells such as white blood cells, red blood cells, and platelets. (medlineplus.gov)
  • Collaborative studies are ongoing to explore the potential of hPSC derivatives such as oligodendrocytes, Schwann cell precursors, and peripheral neurons. (sloankettering.edu)
  • Here, we established a hPSC line integrated with a mCherry fluorescent protein driven by the alpha myosin heavy chain (aMHC) promoter, which could be used to purify CMs based on the aMHC promoter activity in these cells. (biomedcentral.com)
  • This pre-patterned ectoderm can be used to investigate the cell sorting behavior of hPSC-derived meso- endoderm cells , with an endoderm that segregates from the neural ectoderm . (bvsalud.org)
  • In a single experiment, cells that shared a progenitor were more transcriptionally similar to each other than to other cells in the differentiated population. (figshare.com)
  • To varying degrees, these fates also extend to the Such state stability is required in stem and progenitor cells to immediate progeny of stem cells, known as progenitor or support self-renewal and maintenance of the uncommitted transit-amplifying cells. (lu.se)
  • whole well image of culture well (96 well plate format) containing human induced pluripotent stem cells grown on mouse feeders, stained for pluripotent markers NANOG (red) and OCT4 (green). (lu.se)
  • Our lab has worked for more than a decade on developing a cell therapy for Parkinson's disease (PD). (sloankettering.edu)
  • The main objective of the Stem Cell CenterĀ“s Neuroscience Program is to study the properties of neural stem cells (NSCs) and explore the possibilities to use these cells for treatment of neurodegenerative diseases such as Parkinson's disease and stroke. (lu.se)
  • Cells were grown in a directed, stepwise manner following established protocols. (indianactsi.org)
  • However, current protocols for deriving neurons from human pluripotent stem cells give rise to heterogeneous cell populations both in regard to the temporal aspects and the cellular composition. (lu.se)
  • We provide detailed protocols for the generation of lentiviral vectors, the transduction of hES-cells, and their differentia- tion into neurons. (lu.se)
  • Finally, we provide protocols for analyzing these cells through flow cytometry and immunocytochemistry. (lu.se)
  • We have a new publication out ( direct link ), Photoreceptor Outer Segment-like Structures in Long-Term 3D Retinas from Human Pluripotent Stem Cells authored by Karl Wahlin , Julien Maruotti , Srinivasa Sripathi , John Ball, Juan Angueyra, Catherin Kim, Rhonda Grebe, Wei Li , myself and Don Zack . (utah.edu)
  • 5. 293T cells - must never be grown to confluency. (lu.se)
  • Insights from those studies should go toward further improving cell therapy for PD in the future. (sloankettering.edu)
  • The work with stem cell-based therapy in stroke is in initial phase and recently Dr. Zaal Kokaia started a consortium within EU to develop the preclinical protocol for cell therapy in stroke. (lu.se)
  • Prof. Lindvall and Dr. Kokaia were the first in the world to show that the brain produces new neurons in response to stroke which opened new possibilities for cell therapy by developing the way to modulate and direct brains this self-repair mechanisms. (lu.se)
  • The development of human induced pluripotent stem cell (iPSC)-derived cell lines as models for drug-toxicity testing offers a promising alternative that is more physiologically relevant, more predictive, and more time and cost efficient. (the-scientist.com)
  • Based on those results, we are currently pursuing the development of a clinical-grade dopamine neuron cell product and are in the process of generating the data necessary for an IND (investigational new drug) application from the FDA, which is required for the first human clinical use of human ESC-derived dopamine neurons. (sloankettering.edu)
  • Thorold Theunissen, Ph.D., of the Washington University School of Medicine in St. Louis shares his work using naive stem cells to model trophoblast development. (ucsd.tv)
  • We are moving a step closer to understanding how to leverage our knowledge of human development to improved access to mature human cell types. (azolifesciences.com)
  • Development and use of induced stem cells is still considered experimental. (msdmanuals.com)
  • Although the source of induced pluripotent stem cells does not raise unique ethical concerns, there are other ethical issues around related to the experimental use of human pluripotent stem cells whether they are derived from embryos or adults. (gc.ca)
  • Stem cells can be obtained from the embryos that are not used. (msdmanuals.com)
  • Because the embryos then lose the ability to grow into a complete human being, the use of stem cells from embryos is controversial. (msdmanuals.com)
  • Cancer cells continue to produce lactate via glycolysis when oxygen is abundant (the Warburg effect) ( 14 ). (spandidos-publications.com)
  • Similarly, destruction of neonatally abundant pluripotent stem cells would likely have a more pervasive outcome than destruction of The value of incorporating immunologic appeared more severe and/or persistent when single lineages or differentiated cells that pre- data for the toxicologic assessment of drugs, the exposure occurred perinatally when com- dominate in adults. (cdc.gov)
  • Moreover, single cell heterogeneity amongst stem cell populations may underlie important cell fate decisions. (jci.org)
  • Here we describe how microRNA-regulated lentiviral vectors can be used to visualize specific cell populations by exploiting endogenous microRNA expression patterns. (lu.se)
  • To track differentiating cell populations, reporter cell lines generated by homologous recombination (knock-in) or via BAC transgenes have been widely used in mouse cells (5-8 ). (lu.se)
  • In addition to pursuing the first clinical application of human ESC-derived dopamine neurons, we continue to work on detailing the mechanisms of graft function by implementing novel tools such as optogenetics, a technology that enables "switching off" or "switching on" grafted cells in freely moving animals . (sloankettering.edu)
  • The biological properties and clinical potential of stem cells elicit that are generated must not be unduly sensitive to small fluctu- continued scientific, commercial, and public interest. (lu.se)
  • 261-266 ), and Dr Cooper is actively pursuing sponsorship to begin clinical trials in humans. (medscape.com)
  • Other iPSC-based models affecting neural crest lineages include Schwann cell and melanocyte-related disorders, including our effort to model human melanoma in pluripotent stem cells. (mskcc.org)
  • Disorders of the oesophagus and trachea are prevalent enough in people that organoid models of human oesophagus could be greatly beneficial," said Jim Wells, PhD , the Chief Scientific Officer at CuSTOM and study lead investigator. (drugtargetreview.com)