Human stem cell differentiation differentiation of cells. Medical search. Frequent questions

MSCs are a population of nonhematopoietic adult stem cells that have the property of self-renewal and can differentiate into multiple lineages [ 8 - 10 ]. (hindawi.com)
Hematopoietic stem cells (HSC) give rise to all types of blood lineages, including red blood cells (RBC). (lu.se)
Thus, our work highlights similarities in mitochondrial remodeling during the transition from pluripotent to multipotent state in ectodermal and mesodermal lineages, while at the same time demonstrating cell-lineage-specific adaptations upon further differentiation. (uib.no)
Gene expression at these histones regulated by epigenetic modifications is critical in restricting the embryonic stem cell to desired cell lineages and developing cellular memory. (wikipedia.org)
Moreover, we hypothesized that the high glucose, pyruvate and oxygen concentrations found in typical growth media could inhibit the differentiation of certain lineages. (authorea.com)
A unique and essential property of embryonic stem cells is the ability to self-renew and differentiate into multiple cell lineages. (biomedcentral.com)
All cell lines differentiated into three embryonic germ lineages in embryoid bodies and into neural cell lineages when cultured in neural differentiation medium. (biomedcentral.com)
However, current approaches for chondrogenic differentiation of hiPSCs are complicated and inefficient primarily due to intermediate embryoid body formation, which is required to generate endodermal, ectodermal, and mesodermal cell lineages. (mdc-berlin.de)
Second, altering the MSC state by inducing differentiation into multiple lineages did not affect the exosomal properties or endocytosis but triggered the expression of lineage-specific genes and proteins in vitro and in vivo respectively. (frontiersin.org)
SHED has the ability to be differentiated to specific cell lineages such as odontoblasts and osteoblasts as well as epithelial like cells. (bvsalud.org)

In this thesis work, human MSCs (hMSCs) were differentiated into DA neurons on four different carbon-based materials and the differentiation process with and without differentiation factors was assessed by following markers related to neurogenesis. (aalto.fi)
These experiments demonstrate various challenges related to developing an efficient protocol for DA differentiation from hMSCs, the most important being the optimization of the combined mechanical and chemical stimuli. (aalto.fi)
Here we show that graphene provides a promising biocompatible scaffold that does not hamper the proliferation of human mesenchymal stem cells (hMSCs) and accelerates their specific differentiation into bone cells. (elsevierpure.com)
Human mesenchymal stem cells (HMSCs) are multipotent somatic stem cells that can be isolated from a variety of tissues such as the bone marrow, adipose tissue, and dental pulp. (frontiersin.org)
Kim and Park, 2017 ) and make HMSCs attractive cells for regenerative therapies. (frontiersin.org)
The aim of this study was to investigate the osteogenic differentiation potential of human mesenchymal stem cells (hMSCs) cultivated on silk-fibroin films in response to different parameters, i.e. (tubitak.gov.tr)
Researchers from the West Virginia University School of Medicine demonstrate that osteoblasts-derived (Os-Exo) and adipocytes-derived (Ad-Exo) exosomes contain instructive factors that regulate the lineage specification of human mesenchymal stem cells (hMSCs). (exosome-rna.com)
Kinetic and differentiation analyses indicate that both osteoblast and adipocyte exosomes augment ECM-mediated differentiation of hMSCs into the respective lineage. (exosome-rna.com)

The results showed that the space microgravity environment provided favorable conditions for the 3D growth and maintenance of stemness of mouse embryonic stem cells, and stem cells cultured in space showed a better 3D growth mode than on the ground and maintained a higher level of pluripotent gene expression. (pdnews.cn)
Recent ChIP experiments of human and mouse embryonic stem cells have elucidated the architecture of the transcriptional regulatory circuitry responsible for cell determination, which involves the transcription factors OCT4, SOX2 and NANOG. (lu.se)
the EST, which assesses the effects of compounds on the differentiation of mouse embryonic stem cells into contracting cardiomyocytes, can be used to rank the potency of chemicals within a series of alkoxyacetic acid metabolites formed from The implementation of the European REACH (Registration, glycol ethers. (cdc.gov)

In contrast to recent findings in neuronal differentiation, we found that mitochondrial content decreases continuously during mesoderm differentiation, despite increased mitochondrial activity and higher levels of ATP-linked respiration. (uib.no)
Immunocytochemistry and quantitative real-time polymerase chain reaction analysis demonstrated that neuronal differentiation of hiPSC-derived neuronal precursors was enhanced after treatment with 1-2 μg/ml RA-loaded PCANs. (edu.au)
In contrast, with the appropriate chemical and mechanical stimuli, these cells can be guided towards the neuronal lineage in vitro. (aalto.fi)
The ATC Human Stem Cell Core is currently undergoing reorganization and will soon be merging with the Human Neuronal Differentiation Core at the Jan and Dan Duncan Neurological Research Institute. (bcm.edu)
The new Human Neuronal Differentiation and Stem Cell Core service list will offer the combined list of services developed by the two original cores and will operate under guidelines for BCM Institutional Advanced Technology Cores (ATC) in coordination with NRI/TCH procedures. (bcm.edu)
2001. Subchronic dermal application of N,N-diethyl m-toluamide (DEET) and permethrin to adult rats, alone or in combination, causes diffuse neuronal cell death and cytoskeletal abnormalities in the cerebral cortex and the hippocampus, and purkinje neuron loss in the cerebellum. (cdc.gov)
The focus of my research is to understand cell fate specification in the developing brain and in human neural progenitor cells, using cell-based models of neuronal differentiation. (lu.se)

Reversibly immortalized human umbilical cord-derived mesenchymal stem cells (UC-MSCs) are responsive to BMP9-induced osteogenic and adipogenic differentiation. (stembook.org)
In addition, several miRNAs reported to have osteogenic and adipogenic differentiation potentials are also identified in these exosomes. (exosome-rna.com)

OBJECTIVE: The aim of the study was (i) to measure the impact of nicotine on the proliferation and chondrogenic differentiation of mesenchymal stem cells from the human Wharton's jelly (hWJ-MSCs) into chondrocytes, (ii) to investigate whether the alpha 7 nicotinic acetylcholine receptors (nAChRs) was expressed in hWJ-MSCs and could play a role in the process. (univ-lorraine.fr)
The chondrogenic differentiation grade of hWJ-MSCs induced by TGF beta 3 was assessed by the Sirius red and Alcian blue staining. (univ-lorraine.fr)
We report a new, straightforward and highly efficient approach for chondrogenic differentiation of hiPSCs, which avoids embryoid body formation. (mdc-berlin.de)

Hematopoietic stem/progenitor cells (HSPC) are known to be functionally diverse in terms of their self-renewal potential and lineage output. (lu.se)
Given the considerable interest in using stem cells for modeling and treating disease, it is essential to understand what regulates self-renewal and differentiation. (uib.no)
The involvement of microRNAs (miRNAs) in orchestrating self-renewal and differentiation of stem cells has been revealed in a number of recent studies. (muni.cz)
Despite growing experience and knowledge concerning human MSC and their use in cell-based strategies, the molecular mechanisms that govern MSC self-renewal, expansion and multilineage differentiation are not well understood and remain an active area of investigation. (biomedcentral.com)
Key signaling pathways are highlighted, and the regulation of ES cell self-renewal and somatic cell reprogramming is discussed. (tocris.com)
This poster summarizes some key protocols demonstrating the use of small molecules across the stem cell workflow, from reprogramming, through self-renewal, storage and differentiation to verification. (tocris.com)
Our comparative analysis provides further evidence for similarities and differences between three hESC lines in self-renewal, and spontaneous and directed differentiation. (biomedcentral.com)
The switch stabilizes the expression levels of the three genes, and through their regulatory roles on the downstream target genes, leads to a binary decision: When OCT4, SOX2 and NANOG are expressed and the switch is ON, the self-renewal genes are ON and the differentiation genes are OFF. (lu.se)
Finally, there is a discussion about the mechanisms behind the self-renewal and differentiation of stem cells and the role of stem cells in renewal of the body's tissues. (lu.se)
SCs are not only capable of maintaining themselves (self-renewal) but also give rise to a proliferative 'transit amplifying' compartment and ultimately to postmitotic differentiated cells with specialized functions. (medscape.com)
By contrast, transit amplifying cells and differentiated cells are incapable of self-renewal and tissue regeneration. (medscape.com)
Furthermore, pathways controlling the self-renewal capacity of normal tissue SCs are frequently deregulated in human cancer. (medscape.com)

Abstract Background Cardiac differentiation of human-induced pluripotent stem (hiPS) cells consistently produces a mixed population of cardiomyocytes and non-cardiac cell types, even when using well-characterized protocols. (figshare.com)
abstract = 'Mitochondrial dysfunction has been demonstrated to result in premature aging due to its effects on stem cells. (ku.dk)

Finally, we used this approach to encapsulate human Neural Stem Cells (hNSC) derived from human Induced Pluripotent Stem Cells (hIPSC), which were further differentiated into neurons within the capsules with negligible loss of viability. (rsc.org)
Human embryonic stem cells (hESCs) and their derived products offer great promise for targeted therapies and drug screening, however, the hESC differentiation process of mature neurons is a lengthy process. (northumbria.ac.uk)
This protein is found in nerve cells (neurons) in the brain, primarily during development before birth. (medlineplus.gov)
The cation flow also plays a role in the process by which the neurons mature to carry out specific functions (differentiation). (medlineplus.gov)
Our current focus is to learn how to direct and efficiently drive controlled differentiation of human stem cells into subtype-specific neurons. (lu.se)
We also develop technologies for direct conversion of human fibroblasts into functional and subtype-specific neurons in vitro, and the conversion of endogenous glia into neurons in vivo. (lu.se)
In this article, we first provide a short history of cell therapy in Parkinson's disease and briefly describe the current state-of-art regarding human stem cell-derived dopamine neurons for use in any patient trial. (lu.se)
Several academic and industry efforts are well under owned subsidiary Cyto Therapeutics, it had received way to produce dopaminergic neurons from stem approval by the Australian government to conduct a cells under conditions compliant with use in patients. (lu.se)
Without this, the patient cells lost in PD could be replaced by grafted community is left trying to interpret complex scien- immature human dopaminergic neurons [3, 5]. (lu.se)

Recently, a number of studies have confirmed that ADSCs possess the ability to differentiate into adipocytes, osteoblasts, and chondrocytes [ 16 - 18 ], suggesting that a broader source of stem cells is available for application in tissue engineering. (hindawi.com)
Below, we outline a method that is based on published protocols as well as our own experience by which one can differentiate hPSCs along the pancreatic lineage to generate insulin-producing beta-cell-like cells. (ca.gov)
We aim to develop a cell culture platform to automatically differentiate human induced pluripotent stem cells (hiPSCs) for lab-on-a chip applications. (dim-elicit.fr)
Under these conditions, iPS cells begin to differentiate into a hepatocyte lineage. (spandidos-publications.com)
Human induced pluripotent stem (iPS) cells can differentiate into somatic cells ( 1 ). (spandidos-publications.com)
Mesenchymal stem cells are pluripotent progenitor cells that have the capacity to differentiate into several mesenchymal tissues: osteoblasts, chondrocytes and adipocytes. (3hbiomedical.com)
This regulation of chromatin through epigenetic modifications is a molecular mechanism that determines whether the cell continues to differentiate into the desired fate. (wikipedia.org)
Embryonic stem cells containing reduced or elevation levels of methylation are viable but unable to differentiate and therefore require critical regulation of cytosine methylation for mammalian development. (wikipedia.org)
Human mesenchymal stem cells (MSC) with the capacity to differentiate into osteoblasts provide potential for the development of novel treatment strategies, such as improved healing of large bone defects. (biomedcentral.com)
Mesenchymal stem cells (MSCs) are multipotent cells that can differentiate into various cell types depending on their environment, but cannot undergo neurogenesis in normal conditions. (aalto.fi)
They can differentiate into cells of the mesodermal lineage, such as adipocytes, and osteocytes. (tocris.com)
Under the same neural differentiation-promoting conditions, the ability of each cell line to differentiate into neural progenitors varied. (biomedcentral.com)
Human embryonic stem cells (hESCs) possess the ability to self-renew in an undifferentiated state in culture while retaining the ability to differentiate into all of the cell types in the human body. (biomedcentral.com)
It is also essential to understand how the inherited variation in the sex, stage, quality and genetic background of embryos, as well as environmental influences such as derivation methods and passage procedures can affect the ability of hES cell lines to self-renew and differentiate. (biomedcentral.com)
In the present study, a side-by-side comparison of the ability to maintain an undifferentiated state and to self-renew under standard conditions, the ability to spontaneously differentiate into cell types of three germ layers in embryonic bodies, and directed differentiation under neural differentiation-promoting conditions was made between three NIH registered hESC lines I3, I6 and BG01V. (biomedcentral.com)
This stem cell will further mature and differentiate to form a group of hematopoietic stem cells resembling a cluster of grapes. (pdnews.cn)
SHED was able to differentiate into epithelial like cells when cultured in keratinocyte growth medium (KGM) 2 . (bvsalud.org)
Watch the lecture where Malin Parmar tells about the long history of transplantation trials in Lund and how they have been able to differentiate stem cells into specific dopaminergic cells in vitro. (lu.se)

Australia allowed the other download Human Embryonic and Induced Pluripotent Stem Cells: Lineage Specific Differentiation Protocols to impose software for methods in 1902 and Canada was also system centuries as emotional Goodreads by video until 1922. (idealnaja.pl)
Both natural and synthetic materials have been fabricated for transplantation of stem cells and their specific differentiation into muscles, bones, and cartilages. (elsevierpure.com)
Many existing tissue-engineering approaches focus on delivery of selected proteins (growth factors, transcription factors etc.) or nucleic acids to host or implanted stem cells to achieve lineage specific differentiation. (frontiersin.org)
Aim: To detect the expression of molecules associated with Notch signaling pathway in stem cells from human exfoliated deciduous teeth (SHED) cultured in specific differentiation medium, namely, keratinocyte growth medium (KGM). (bvsalud.org)
The aim of this study was to detect the expression of molecules associated with Notch signallng pathway in SHED cultured in specific differentiation medium, namely, KGM. (bvsalud.org)

Here, we studied the effect of several major green tea polyphenols on adipocyte differentiation in human bone marrow mesenchymal stem cells (hBM-MSCs) and compared it to the effect of representative antidiabetic drugs. (nih.gov)
Over the last decades, mesenchymal stem cells (MSCs) have attracted extensive attention in the field of bone regeneration [ 4 - 7 ]. (hindawi.com)
Among these, adipose-derived stem cells (ADSCs) are a type of mesenchymal stem cell isolated from adipose tissue, which has the advantages of abundant storage in vivo , easy acquisition, and expansion [ 13 - 15 ]. (hindawi.com)
Mesenchymal stem/stromal cells (MSC) are the ideal source to study fat formation as they are the progenitors of adipocytes. (mdpi.com)
This study demonstrates the use of a microfluidic platform for long-term cultivation (3 weeks) of human mesenchymal stem-like cells (MSCs), a cell population of high interest for tissue engineering. (rsc.org)
Human liver organoids are formed from the assembly of hepatocyte-like cells differentiated from iPS cells, human umbilical vascular endothelial cells and mesenchymal stem cells ( 9 ). (spandidos-publications.com)
Our mesenchymal stem cell osteogenic differentiation medium has been optimized for the osteogenic differentiation of mesenchymal stem cell (MSCs) in tissue culture plastic-ware. (3hbiomedical.com)
Efficiency of osteogenic differentiation depends on the quanlity of the mesenchymal stem cells. (3hbiomedical.com)
Mesenchymal stem cell Osteogenic Differentiation Medium (MODM) is a sterile, liquid medium which contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors, trace minerals. (3hbiomedical.com)
No. 0025), 5 ml of mesenchymal stem cell osteogenic differentiation supplement (MODS, Cat. (3hbiomedical.com)
In recent years mesenchymal stem cells (MSC) have generated a great deal of interest as a potential source for cell-based therapeutic strategies. (biomedcentral.com)
Bone marrow-derived mesenchymal stem cells (MSC) have been promoted for multiple therapeutic applications. (nih.gov)
The behavior of mesenchymal stem cells from an umbilical cord vein was evaluated using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, a mineralization assay, and an alkaline phosphatase activity assay and by carrying out a quantitative real-time polymerase chain reaction for osteogenic markers. (biomedcentral.com)
Cell proliferation analysis revealed that the CHO-k1 and mesenchymal stem cells behaved differently. (biomedcentral.com)
The laser-processed titanium surface increased the proliferation of CHO-k1 cells, reduced the proliferation of mesenchymal stem cells, upregulated the expression of the osteogenic markers, and enhanced alkaline phosphatase activity. (biomedcentral.com)
Human bone marrow mesenchymal stem cells (hBM-MSCs) are the most commonly used cells. (biomedcentral.com)
Mesenchymal Stem Cells (MSCs) are a heterogeneous subset of stromal stem cells that originate from the mesoderm layer of the fetus. (tocris.com)
Tocris offers the following scientific literature for Mesenchymal Stem Cells to showcase our products. (tocris.com)
We differentiated hiPSCs directly into mesenchymal stem /stromal cells (MSC) and chondrocytes. (mdc-berlin.de)
Mesenchymal stem cells (MSCs) are multipotent cells with regenerative and immunomodulatory properties. (frontiersin.org)
Osteogenic differentiation of electrostimulated human mesenchymal stem" by ANIL SERA ÇAKMAK, SONER ÇAKMAK et al. (tubitak.gov.tr)

Among them is the first international research on human pluripotent stem cells in vitro hematopoietic differentiation of human embryonic stem cells under space conditions. (pdnews.cn)
In an interview with CCTV News, Lei Xiaohua, a researcher at the Institute of Biomedicine and Technology of Chinese Academy of Sciences, stated that the taikonauts aboard the Shenzhou-15 have successfully differentiated a hematopoietic stem cell similar to a cobblestone in orbit. (pdnews.cn)
From the experimental results, China has successfully achieved the first hematopoietic differentiation of human embryonic stem cells under space conditions, accomplishing its first experimental goal. (pdnews.cn)
Lei said that in the next phase, the research team needs to conduct comprehensive detection and analysis of this group of hematopoietic stem cells, and screen out the relevant genes affecting the early hematopoiesis differentiation of human pluripotent stem cells in the space environment by comparing with the ground control group. (pdnews.cn)
Hematopoietic stem cell transplantation (HSCT) involves the intravenous infusion of hematopoietic stem cells in order to reestablish blood cell production in patients whose bone marrow or immune system is damaged or defective. (medscape.com)
May push the resting hematopoietic stem cells into cycle, making them more responsive to differentiation by hematopoietic growth factors. (medscape.com)

The experimental groups can be defined as high voltage in osteogenic differentiation medium, low voltage in osteogenic differentiation medium, and low voltage in growth medium in this study. (tubitak.gov.tr)
Compared to the unstimulated controls (silk films without platinum wires), low voltage (10 mV) did not influence proliferation, while it enhanced osteogenic differentiation according to early and late osteogenic markers in osteogenic differentiation medium. (tubitak.gov.tr)

During liver development, cytokines released from various cell types are critical for stem/progenitor cell differentiation and lineage expansions. (elsevierpure.com)
These beads allow midbrain dopaminergic progenitor cell differentiation and cryopreservation without digestion , effectively maintaining axonal integrity in vitro . (bvsalud.org)

Here, we address the question of mitochondrial adaptation by examining the differentiation of human pluripotent stem cells to cardiac progenitors and further to differentiated mesodermal derivatives, including functional cardiomyocytes. (uib.no)

To determine mechanisms in these stage-specific lineage interactions, we modeled potential effects of soluble signals derived from immortalized human fetal liver parenchymal cells on stem cells, including embryonic and induced pluripotent stem cells. (elsevierpure.com)
These links are download Human Embryonic and Induced Pluripotent Stem Cells: year, ideas, time drag-and-drop, sentence of the market, etc. (idealnaja.pl)
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Many research groups are engaged in using human pluripotent stem cells (hPSCs) to generate surrogate pancreatic beta-cells for transplantation into diabetic patients. (ca.gov)
Hepatocytes derived from iPS cells are suitable for transplantation into patients with liver insufficiency and for use in toxicity tests ( 2 ). (spandidos-publications.com)
Cell transplantation studies were performed in mice with drug-induced acute liver failure to elicit benefits in hepatic support and tissue regeneration. (elsevierpure.com)
Transplantation of midbrain dopaminergic progenitor cells is a promising treatment for Parkinson's disease . (bvsalud.org)
However, transplanted cells can be injured by mechanical damage during handling and by changes in the transplantation niche. (bvsalud.org)
Recent news of an impending clinical cell transplantation trial in Parkinson's disease using parthenogenetic stem cells as a source of donor tissue have raised hopes in the patient community and sparked discussion in the research community. (lu.se)
Based on discussions held by a global collaborative initiative on translation of stem cell therapy in Parkinson's disease, we have identified a set of key questions that we believe should be addressed ahead of every clinical stem cell-based transplantation trial in this disorder. (lu.se)

Fig. 3: Single-cell transcriptomics of dorsal and ventral MiSTR tissues. (nature.com)
Stem cells have potential as a source of cells and tissues for research and treatment of disease. (tocris.com)
Different types of hESCs derived cell products can be used for cell therapy procedures aiming to regenerate functional tissues in patients who suffer from various degenerative diseases. (northumbria.ac.uk)
Notch-1, Jagged-1, Jagged-2, and stem cell marker Nanog are expressed in SHED cultured in KGM which may be involved in the differentiation into epithelial-like cells in human dental pulp tissues. (bvsalud.org)
Stem cells from human exfoliated deciduous teeth (SHED) are multipotent stem cells derived from the pulp tissues of extracted deciduous teeth 1 . (bvsalud.org)
Notch signaling pathway is also involved in the regulation of epithelial cell differentiation in various tissues 5-6 . (bvsalud.org)
Normal proliferative tissues contain multipotent stem cells (SCs) that drive tissue turnover. (medscape.com)
According to the cancer stem cell (CSC) concept, the cellular hierarchy in normal tissues is preserved in a number of hematological and solid malignancies. (medscape.com)

However, for cell therapies MSC have to be expanded and/or manipulated to obtain a sufficient amount of cells that can be subsequently used for treatment. (biomedcentral.com)
We concluded that hepatic differentiation of pluripotent stem cells with metabolomics products will be significant for developing therapies. (elsevierpure.com)
These unique capabilities make hESCs a renewable source of a wide range of cell types for potential use in research and cell-based drug screening and therapies for many diseases. (biomedcentral.com)
The true opportunity of this work lies in the potential to deliver patient-specific treatments as well as curative stem cell-based therapies to patients globally. (lu.se)
My work focus on cell repair and cell models which is central to these aims both for developing new therapies and for understanding and diagnosis disease. (lu.se)
Are Stem Cell-Based Therapies for Parkinson's Disease Ready for the Clinic in 2016? (lu.se)
Stem cell-based therapies for Parkinson's dis- ogy company International Stem Cell Corporation ease (PD) are rapidly moving towards clinical trials. (lu.se)

By transporting "cargos" such as proteins, RNAs, DNAs, and lipids [ 26 ], exosomes regulate the eventual fate of recipient cells. (hindawi.com)
Remodeling of mitochondria and metabolism, with the shift from glycolysis to oxidative phosphorylation (OXPHOS), plays a fundamental role in maintaining pluripotency and stem cell fate. (uib.no)
And while in human pluripotent stem cells, miRNAs have been directly linked to the core pluripotency network, including the cell cycle regulation and the maintenance of the self-renewing capacity, their role in the onset of differentiation in other contexts, such as determination of neural cell fate, remains poorly described. (muni.cz)
The comprehensive miRNA profiling presented here reveals novel sets of miRNAs differentially expressed during human neural cell fate determination in vitro. (muni.cz)
We report thermo-responsive retinoic acid (RA)-loaded poly(N-isopropylacrylamide)-co-acrylamide (PNIPAM-co-Am) nanoparticles for directing human induced pluripotent stem cell (hiPSC) fate. (edu.au)
Embryonic stem cells are capable of self-renewing and differentiating to the desired fate depending on their position in the body. (wikipedia.org)
Since there is also an increase throughout the six-day period in H3K9me, a marker for active heterochromatin, once differentiation occurs it is concluded that the formation of heterochromatin occurs as the cell is differentiated into its desired fate making the cell inactive to prevent further differentiation. (wikipedia.org)
This process, however, requires optimal growth conditions, as well as suitable substrates for controlling the cell fate. (aalto.fi)
Endogenous cell signals regulate tissue homeostasis and are significant for directing the fate of stem cells. (elsevierpure.com)
Lineage specification is an essential process in stem cell fate, tissue homeostasis and development. (exosome-rna.com)
The Notch signaling pathway provides important intercellular signaling mechanisms essential for cell fate specification and it regulates differentiation and proliferation of stem or progenitor cells by para-inducing effects 3-4 . (bvsalud.org)

Additionally, we were able to reconstruct the three developmental phases during osteoblast differentiation: proliferation, matrix maturation, and mineralization, and illustrate the activation of the SMAD signaling pathways by TGF-β2 and BMPs. (biomedcentral.com)
The combination of osteoblast/adipocyte ECM and exosomes turned-on the lineage specific gene expressions at earlier time points of differentiation compared to the respective ECM or exosomes administered individually. (exosome-rna.com)

By immunostaining for cell type specific markers, I have documented the different stages of retinal differentiation and provided insight into the mechanisms of ipRGC differentiation. (indianactsi.org)
Stem cell homeostasis is maintained through epigenetic mechanisms that are highly dynamic in regulating the chromatin structure as well as specific gene transcription programs. (wikipedia.org)
There has been multiple evidence suggesting that the maintenance of the lineage commitment of stem cells is controlled by epigenetic mechanisms such as DNA methylation, histone modifications and regulation of ATP-dependent remolding of chromatin structure. (wikipedia.org)
Although MSC-derived EVs (mEVs) are beneficial for acute lung injury and pulmonary fibrosis, mechanisms of mEV uptake by lung fibroblasts and their effects on myofibroblastic differentiation have not been established. (nih.gov)
The differentiation mechanisms involving metabolomics products could have an impact on advancing recruitment of stem/progenitor cells during tissue homeostasis. (elsevierpure.com)
Acceleration mechanisms for neural differentiation have been explored by bulk RNA sequencing, quantitative polymerase chain reaction (qPCR) and immunostaining. (northumbria.ac.uk)
Statement of significance: Human embryonic stem cells (hESC) are used for investigating the complex mechanisms involved in the development of specialized biological cells and organs. (northumbria.ac.uk)
Although MSC EVs show great promise for regenerative medicine applications, insights into their uptake mechanisms by different target cells and the ability to control MSC EV properties for defined function in vivo have remained elusive knowledge gaps. (frontiersin.org)
The course also includes how developmental biology knowledge can be utilised in the establishment of animal models for studies of human disease mechanisms. (lu.se)

Experts said that the unique space microgravity environment may be a new way to maintain undifferentiated proliferation of stem cells, enhance the efficiency of induced differentiation, and improve the level of three-dimensional tissue construction. (pdnews.cn)

Frontiers in Cell and Developmental Biology. (uib.no)
My group works with translational stem cell biology. (lu.se)
On completion of the course, the students shall be able to · reflect on societal opportunities and consequences arising from developmental biology research and knowledge, and discuss these with individuals in the same education cycle · reflect on current scientific challenges and ethical issues in developmental and stem cell biology research and how this influences society in general. (lu.se)
The whole course is based on five week-long modules on selected subjects related to issues concerning developmental biology and stem cells. (lu.se)

The slight rebound in each of these marks allows for further differentiation to occur by allowing another opportunity to decrease the markers once again, bringing the cell closer to its mature state. (wikipedia.org)

erythroid cells after in vitro differentiation, despite having similar frequencies of phenotypic HSC initially. (lu.se)
examined the effects of epigenetic modifications on the chromatin structure and the modulation of these epigenetic markers during stem cell differentiation through in vitro differentiation of murine embryonic stem (ES) cells. (wikipedia.org)
Histone modifications of H3K9 position show a decrease in di- and tri-methylation of undifferentiated embryonic stem cells and had a gradual increase in methylation during the six-day time course of in vitro differentiation, which indicated that there is a global increase of inactive heterochromatin levels at this histone mark. (wikipedia.org)

Microarray analysis of MSC during osteogenic differentiation identified three candidate genes for further examination and functional analysis: ID4, CRYAB, and SORT1. (biomedcentral.com)
These data suggest that the interaction of Thy-1 with beta integrins mediates mEV uptake by lung fibroblasts, which blocks myofibroblastic differentiation, and that mEVs are enriched for miRs that target profibrotic genes up-regulated in IPF fibroblasts. (nih.gov)
We thus wondered how cells grow spatially during differentiation, so we labeled cells by expression of marker genes and found that cells expressing the same marker tended to occur in patches. (figshare.com)
In addition to regulating each other through feedback loops, these genes also regulate downstream target genes involved in the maintenance and differentiation of embryonic stem cells. (lu.se)
Gene Therapy Although gene therapy is defined as any treatment that changes gene function, it is often thought of as the insertion of normal genes into the cells of a person who lacks such normal genes because. (msdmanuals.com)
Genes and Chromosomes Genes are segments of deoxyribonucleic acid (DNA) that contain the code for a specific protein that functions in one or more types of cells in the body or the code for functional ribonucleic. (msdmanuals.com)

Bandi, S , Tchaikovskaya, T & Gupta, S 2019, ' Hepatic differentiation of human pluripotent stem cells by developmental stage-related metabolomics products ', Differentiation , vol. 105, pp. 54-70. (elsevierpure.com)

Protocols for hepatocyte differentiation from iPS cells have been studied. (spandidos-publications.com)
Cells were grown in a directed, stepwise manner following established protocols. (indianactsi.org)
Developed in-house, this poster summarizes how small molecules have been used in protocols across the stem cell workflow. (tocris.com)

Most of these paracrine secretions include soluble factors and exosomes, which regulate the repair and regeneration processes at sites of damage by affecting cell proliferation, migration, and differentiation [ 22 , 23 ]. (hindawi.com)

The study of brain development in humans is limited by the lack of tissue samples and suitable in vitro models. (nature.com)
Fig. 1: Design of the MiSTR cell culture system and establishment of a WNT signaling gradient in hESC-derived tissue. (nature.com)
One great advantage of MSC is that these cells may be directly obtained from individual patients, thereby eliminating the complications associated with immune rejection of allogenic tissue and infectious diseases. (biomedcentral.com)
The extent of metabolic and synthetic functions in stem cell-derived hepatocytes was sufficient for providing hepatic support along with promotion of tissue repair to rescue mice in acute liver failure. (elsevierpure.com)
In recent years, foreign scientists have also reported several times on research regarding stem cell growth and tissue regeneration carried out during space flight missions, such as blood stem cells for astronauts with anemia. (pdnews.cn)
Current tissue engineering approaches combine different scaffold materials with living cells to provide biological substitutes that can repair and eventually improve tissue functions. (elsevierpure.com)
The results suggest the potential to exploit exogenous biophysical control of cell functions towards tissue-specific goals. (tubitak.gov.tr)
Biopsy of adipose tissue identified human adenovirus 36 (Adv 36) DNA. (cdc.gov)
Normal multipotent tissue stem cells (SCs) are the driving force behind tissue turnover and repair. (medscape.com)
Whether a cell used for a clone produces a specific type of tissue, a specific organ, or an entire organism depends on the potential of the cell-that is, how highly the cell has developed into a particular type of tissue. (msdmanuals.com)
For example, certain cells called stem cells have the potential to produce a wide variety of tissue types or even possibly an entire organism. (msdmanuals.com)

However, when the same hiPS cells were differentiated in parallel, we did not observe high transcriptional similarity across differentiations. (figshare.com)

We provide intensive hands-on training on human pluripotent stem cell culture for Baylor College of Medicine, MD Anderson, and other Texas Medical Center investigators. (bcm.edu)

To bridge this gap, we used three model cell types to study miRNA expression patterns: human embryonic stem cells (hESCs), hESCs-derived self-renewing neural stem cells (NSCs), and differentiating NSCs. (muni.cz)
Our data also indicates that miRNA clusters enriched in NSCs share the target-determining seed sequence with cell cycle regulatory miRNAs expressed in pluripotent hESCs. (muni.cz)
However, the possible differences in proliferation and differentiation capabilities among independently-derived human embryonic stem cells (hESCs) are not well known because of insufficient characterization. (biomedcentral.com)
Neural differentiation of the hESCs is greatly accelerated after application of the acoustic stimulations. (northumbria.ac.uk)
Accelerating the hESCs' differentiation process can considerably benefit the clinical utilization of these cells. (northumbria.ac.uk)
This study develops a highly effective acoustic stimulator working at ∼20 MHz to investigate what roles do acousto-mechanical stimuli play in the differentiation of hESCs. (northumbria.ac.uk)

These UCNPs allow programmable activation of two synergistic light-gated ion channels VChR1 and Jaws in the same cell to manipulate membrane polarization, demonstrated here for cardiac pacing. (nature.com)

Human MSC are easy to isolate from small aspirate of bone marrow via their adherence ability. (biomedcentral.com)
One of the key objectives for bone regeneration therapy to be successful is to direct stem cells proliferation and to accelerate their differentiation in a controlled manner through the use of growth factors and osteogenic inducers. (elsevierpure.com)
The National Marrow Donor Program (NMDP), founded in 1986, and the World Marrow Donor Association (WMDA), founded in 1988, were established to (1) locate and secure appropriate unrelated-donor HSCT sources for patients by promoting volunteer donation of bone marrow and peripheral blood stem cells in the community and (2) promote ethical practices of sharing stem cell sources by need, rather than by geographic location of the donor. (medscape.com)

The approach, named microfluidic-controlled stem cell regionalization (MiSTR), exposes pluripotent stem cells to signaling gradients that mimic developmental patterning. (nature.com)
Knowledge on the expression analysis of Notch signaling pathway molecules in SHED cultured in KGM could highlight its involvement in controlling the biological activity of these stem cells, particularly during odontogenesis and other developmental process. (bvsalud.org)
PBK model allows the prediction of dose-response curves for implantation rat whole-embryo culture test, the rat limb bud human developmental toxicity. (cdc.gov)

Single-cell transcriptomics revealed that rostro-caudal organization was already established at 24 h of differentiation, and that the first markers of a neural-specific transcription program emerged in the rostral cells at 48 h. (nature.com)
Catechin increased the mRNA levels of various adipogenic markers, such as adiponectin, peroxisome proliferator-activated receptor gamma (PPARgamma), FABP4, and LPL, as measured during adipocyte differentiation in hBM-MSCs. (nih.gov)
After two days of culture in HSM, the expression levels of α-fetoprotein (AFP) and albumin, which are hepatocyte markers, were upregulated, suggesting that hepatocyte differentiation was initiated ( 19 ). (spandidos-publications.com)
citation needed] As the embryonic stem cell undergoes differentiation the markers for active euchromatin (histone acetylation and H3K4 methylation) are decreased after the removal of LIF showing that the cell is indeed becoming more differentiated. (wikipedia.org)
For identifying lineage conversion and maturation, we utilized conventional assays of cell morphology, gene expression analysis and lineage markers. (elsevierpure.com)
Using immunocytochemistry, flow cytometry, qRT-PCR and MPSS, we found that all three cell lines actively proliferated and expressed similar "stemness" markers including transcription factors POU5F1/Oct3/4 and NANOG, glycolipids SSEA4 and TRA-1-81, and alkaline phosphatase activity. (biomedcentral.com)
The neural progenitor cell markers, including Pax6, Sox1, Sox2, Sox10 and Nkx2-1, are less expressed in the SAW dose groups compared with the control group by the qPCR. (northumbria.ac.uk)
On the other hand, high voltage (500 mV) stimulated cell proliferation and only late osteogenic markers in osteogenic medium. (tubitak.gov.tr)
CD133+), but are rarely codetected with the neural stem dents, very few human-specific NSC markers have been cell (NSC) marker CD15. (lu.se)
2000). In *Correspondence to: Perrine Barraud, Department of Veterinary Medi- contrast, several cell surface markers have been used to cine, Neurosciences, Madingley Road, Cambridge CB3 OES, United enrich for NSCs in the rodent CNS. (lu.se)

Our Stem Cells review gives an overview of the use of small molecules in the control of stem cell growth & differentiation and somatic cell reprogramming. (tocris.com)

The results of these analyses revealed that the differentially expressed exosomal miRNAs participate in multiple biological processes, such as gene expression, synthesis of biomolecules, cell development, differentiation, and signal transduction, among others. (hindawi.com)
Cells were initially edited by the CRISPR/Cas9 system to express the Thy1 gene at the Brn3b locus and the tdTomato gene at the melanopsin locus. (indianactsi.org)
Finally we analyzed the gene expression profiles of MSC during osteogenic differentiation. (biomedcentral.com)
Gene expression profiling and comparison of regulatory networks in immature and mature hepatocytes revealed stem cell-derived hepatocytes represented early fetal-like stage. (elsevierpure.com)
In stem cells exposed to signals from fetal cells, mechanistic gene networks of upstream regulators decreased pluripotency, while simultaneously inducing mesenchymal and epithelial properties. (elsevierpure.com)
However, a profound variation in colony morphology, growth rate, BrdU incorporation, and relative abundance of gene expression in undifferentiated and differentiated states of the cell lines was observed. (biomedcentral.com)
Our results show that acoustic dose alters the extracellular matrix and physiological activity-related gene expression, which indicates that the acoustic stimulation is an important tool for regulating the stem cells' differentiation processes in cell therapy. (northumbria.ac.uk)
Many GRIN2B gene mutations lead to production of a nonfunctional GluN2B protein or prevent the production of any GluN2B protein from one copy of the gene in each cell. (medlineplus.gov)
In muscle cells, gene expression of glucose transporters Glut 1 and Glut 4 and phosphoinositide 3-kinase is increased, which results in noninsulin-mediated increases in glucose transport ( 14 ). (cdc.gov)
2. Nuclear transfer is a technique used to duplicate genetic material by creating an embryo through the transfer and fusion of a diploid cell in an enucleated female oocyte.2 Cloning has a broader meaning than nuclear transfer as it also involves gene replication and natural or induced embryo splitting (see Annex 1). (who.int)

During this rescue, paracrine factors from transplanted cells contributed in stimulating liver regeneration. (elsevierpure.com)
Human induced pluripotent stem cells (hiPSCs) have demonstrated great potential for hyaline cartilage regeneration. (mdc-berlin.de)
This can provide more beneficial assistance for the future use of stem cell regeneration to serve human health. (pdnews.cn)
Furthermore, the course covers the most important processes in early embryo development, such as fertilisation, cell division, establishment of position information, polarity and asymmetries, and formation of body axes and gastrulation as preconditions for extremity development, regeneration and formation of the body's most important organs. (lu.se)

The implementation of human pluripotent stem cells (hiPSCs) in cell therapy has an extraordinary potential but faces many practical challenges, including costs associated with growth media and factors. (authorea.com)
There is an immediate need to establish an optimized culture platform to direct the differentiation of hiPSCs into germ layers in a defined nutritional microenvironment to generate cost-effective and robust therapeutics. (authorea.com)
The aim of this study was to identify the optimal nutritional environment by mimicking the in vivo concentrations of three key factors (glucose, pyruvate and oxygen) during the spontaneous differentiation of hiPSCs derived from cord blood, which greatly differ from the in vitro expansion and differentiation scenarios. (authorea.com)
A design of experiments (DoE) was used to investigate the interaction between these three variables during the spontaneous differentiation of hiPSCs. (authorea.com)
Our findings present a novel approach for efficient directed differentiation of hiPSCs through the manipulation of media components whilst simultaneously avoiding the usage of growth factors thus reducing costs. (authorea.com)

Here, we developed a one-step biomanufacturing platform that uses small-aperture gelatin microcarriers to produce beads carrying midbrain dopaminergic progenitor cells . (bvsalud.org)

We illustrated for the first time in a human model the three main stages of osteogenic development, and we could show the diverse regulation of the SMAD pathways by TGF-β2 and BMPs. (biomedcentral.com)
Proteomics is emerging as a powerful tool to identify the signaling complexes and pathways that control multi- and pluri-potency and SC differentiation. (medscape.com)

We present here a microfluidic device that generates sub-millimetric hollow hydrogel spheres, encapsulating cells and coated internally with a layer of reconstituted extracellular matrix (ECM) of a few microns thick. (rsc.org)
Microenvironmental milieu consists of two essential components, one being extracellular matrix (ECM) as the substratum, while the other being cell secreted exosomes and growth factors. (exosome-rna.com)

Unexpectedly, differentiation-inducing soluble signals constituted metabolomics products and not proteins. (elsevierpure.com)
ECM of differentiated cells modulates phenotypic expression of stem cells, while their exosomes contain phenotype specific instructive factors (miRNA, RNA and proteins) that control stem cell differentiation. (exosome-rna.com)
Cancer stem cell-associated proteins revealed by proteomics. (medscape.com)

The results showed that MSC did not alter their osteogenic differentiation capacity, surface marker profile, and the expression profiles of MSC during expansion. (biomedcentral.com)
We monitored these cells during their expansion ex vivo with respect to proliferation kinetics, surface marker profile and differentiation potential. (biomedcentral.com)
In addition, the marker profiles revealed a positive effect of the nanostructures on the MSC differentiation, while PDL coating was found unfavorable for MSCs. (aalto.fi)
Moreover, the immunostaining confirmed that more mature neuron marker Tuj1-positive cells, while less stem cell marker Sox2-positive cells, are presented in the SAW dose groups. (northumbria.ac.uk)
RNA was reverse-transcribed to obtain the cDNA and then proceeded with PCR using specific primers for the Notch signaling pathway molecules (Notch1, Jagged-1, Jagged-2 and, Hes1) as well as stem cell marker (Nanog). (bvsalud.org)
The stage-specific embryonic antigen 4 (SSEA4) is com- isolate the NSCs from neonatal mice and rats (Campos monly used as a cell surface marker to identify the pluri- et al. (lu.se)

However, to our knowledge, there is no report on the successful generation of glucose-responsive insulin-producing beta-cells from hPSCs in vitro. (ca.gov)

Our study reveals especially the transition between hepatic specification and hepatic maturation as dependent on mitochondrial respiration and demonstrates that even though differentiating cells are primarily dependent on glycolysis until induction of hepatocyte maturation, oxidative phosphorylation is essential at all stages of differentiation. (ku.dk)

Catechin was the most potent of the eight green tea polyphenols evaluated in promoting adipocyte differentiation in hBM-MSCs, and this effect was dose-dependent. (nih.gov)
In conclusion, our data suggest that (-)-catechin promotes adipocyte differentiation and increased sensitivity to insulin in part by direct activation of PPARgamma, which could be at the basis of the observed pharmacological benefits of green tea intake in reducing the risk of type 2 diabetes. (nih.gov)

These studies showed signals emanating from fetal liver cells induced hepatic differentiation in stem cells. (elsevierpure.com)

Numerous studies have shown that cell replacement therapy can replenish lost cells and rebuild neural circuitry in animal models of Parkinson's disease . (bvsalud.org)
The ultimate aim is to develop these cells and technologies for use in brain repair, with a focus on Parkinson's disease. (lu.se)
Develop a stem cell-based therapy for Parkinson's Disease. (lu.se)
With this background information as a foundation, we then discuss each of the key questions in relation to the upcoming therapeutic trial and critically assess if the time is ripe for clinical translation of parthenogenetic stem cell technology in Parkinson's disease. (lu.se)

To address this question we first developed human oligonucleotide microarrays with 30.000 elements and then performed large-scale expression profiling of long-term expanded MSC and MSC during differentiation into osteoblasts. (biomedcentral.com)

Here, we model early human neural tube development using human embryonic stem cells cultured in a microfluidic device. (nature.com)

Anatomy64

Organisms9

Diseases6

Chemicals and Drugs81

Analytical, Diagnostic and Therapeutic Techniques and Equipment36

Phenomena and Processes58

Disciplines and Occupations4

Technology, Industry, Agriculture4

Information Science3

Lineages10

HMSCs8

Mouse embryonic stem3

Neuronal7

Adipogenic Differentiation2

Chondrogenic differentiation3

Self-renewal12

Abstract2

Neurons9

Differentiate18

Specific differentiation5

Mesenchymal Stem21

Hematopoietic6

Osteogenic Differentiation Medium2

Progenitor cell differentiation2

Cardiomyocytes1

Embryonic and induced pluri3

Transplantation7

Tissues8

Therapies7

Fate11

Osteoblast2

Mechanisms9

Proliferation of stem1

Biology4

Further differentiation1

Vitro differentiation3

Genes6

20191

Protocols3

Affecting cell proliferation1

Tissue11

Transcriptional1

Pluripotent stem1

HESCs6

Cardiac1

Bone3

Developmental3

Markers10

Somatic1

Gene10

Regeneration4

HiPSCs5

Dopaminergic1

Pathways2

Extracellular matrix2

Proteins3

Marker6

HPSCs1

Maturation1

Adipocyte2

Fetal liver1

Parkinson's4

Osteoblasts1

Microfluidic device1