• Notwithstanding these lesions, RF-EME exposure did not impair the fertilization competence of spermatozoa nor their ability to support early embryonic development. (nature.com)
  • DNA damages present in spermatozoa in the period after meiosis but before fertilization may be repaired in the fertilized egg, but if not repaired, can have serious deleterious effects on fertility and the developing embryo. (wikipedia.org)
  • On the one hand, reactive oxygen species (ROS) are mandatory mediators for essential cellular functions including the function of germ cells (oocytes and spermatozoa) and thereby the fertilization process. (hindawi.com)
  • This has been shown for intrauterine insemination (IUI), in vitro fertilization (IVF), and ICSI, and increased incidences of chromosomal abnormalities, minor and major birth defects, or early childhood cancers, particularly in the male offspring, have been linked to these procedures [ 8 - 16 ]. (hindawi.com)
  • This research has been conducted at the laboratory of in vitro fertilization of the University ofWisconsin, USA. (lipi.go.id)
  • 1995. Effects of ovary tipe, oocytes grade, hormone, sperm concentration and fertilization medium on in vitro maturation, fertilization and development of bovine follicular oocytes. (lipi.go.id)
  • Maledependent variability of fertilization and embryo development in two bovine in vitro fertilization systems and the effects of casein phosphopeptides (CPPs). (lipi.go.id)
  • Current status of in vitro maturation (IVM) and in vitro fertilization (FIV) and embryo culture in human and farm animal. (lipi.go.id)
  • Lentivirally-transduced mouse spermatozoa were used in in vitro fertilization studies and when followed by embryo transfer, at least 42 percent of founders were transgenic for GFP. (science20.com)
  • A University of Chicago 2018 study of biologists from over 1,000 institutions shows 95% of 5,500 biologists know that human life begins at fertilization . (americanrtl.org)
  • So here we summarize references that address this matter for both sexual (fertilization) and asexual (twinning, cloning, etc.) human reproduction. (americanrtl.org)
  • Fertilization is the procession of events that begins when a spermatozoon makes contact with an oocyte or its investments and ends with the intermingling of maternal and paternal chromosomes at metaphase of the first mitotic division of the zygote (Brackett et al. (americanrtl.org)
  • Abstract] The acrosome reaction is a highly regulated exocytotic event that primes spermatozoa for successful fertilization. (bio-thing.com)
  • Having shed their acrosome, spermatozoa are then capable of penetrating the outer vestments of the oocyte and initiating fertilization. (bio-thing.com)
  • We investigated whether the insemination method ( in vitro fertilization [IVF] or intracytoplasmic sperm injection [ICSI]) affected morphokinetic events and abnormal cleavage events in embryonic development. (ecerm.org)
  • Since the first in vitro fertilization-embryo transfer (IVF-ET) baby was born in 1978, IVF-ET procedures have made remarkable progress, and various assisted reproductive technologies have been implemented. (ecerm.org)
  • We hope this line of research will eventually result in strategies that will enable us to use the healthiest spermatozoa for fertilization to improve the chances of pregnancy. (mensfe.net)
  • The fertilizing potential of sperm depends on the shape of the spermatozoa, the ability of the spermatozoa to perform the functions necessary for fertilizing an egg, and finally, the transfer of intact genetic material (DNA) to the egg at the time of fertilization. (mensfe.net)
  • Women trying to conceive or undergoing In Vitro Fertilization (IVF) treatment may benefit from using vitamin and anti-oxidant therapy. (fertilevitamin.com)
  • Pregnancy rates may be lower in women with low vitamin D levels undergoing In Vitro Fertilization (IVF) treatment. (fertilevitamin.com)
  • The authors concluded that these findings provide new insights to improve sperm cryopreservation and in vitro fertilization in the domestic and wild felids and human. (alphanano.tech)
  • in vitro fertilization. (who.int)
  • In vitro maturation strategies are currently being optimized in animal models. (elifesciences.org)
  • 2001. Comparisons of oocytes maturation times and three methods of sperm preparation for their effects on the production of goat embryos in vitro. (lipi.go.id)
  • As a result of spermatogenesis and epididymal maturation, male germ cells differentiate to become fully functional spermatozoa. (biomedcentral.com)
  • Conceive Plus® is compatible with human sperm, oocytes and embryos and safe for use by trying to conceive couples. (conceiveplus.com)
  • The oocytes were fertilized in vitro with motile sperm selected and capacitated by using the percoll gradient with 2 ml vs 0.5 ml per layer as treatment A and B respectively. (lipi.go.id)
  • Effects of cumulus cells on male pronuclear formation and subsequent early development of bovine oocytes in vitro. (lipi.go.id)
  • 1972). In the case of human oocytes fertilized in vitro, pronuclei were formed within 11 hours of insemination (Edwards, 1972). (americanrtl.org)
  • 2004). In vivo and in vitro differentiation of male germ cells in the mouse. (edu.au)
  • 5,6], hyaluronic acid effectively supports dium supplemented with hyaluronic acid) mouse and human embryo development and and a control Group B (whose embryos also their growth [7,8]. (who.int)
  • Relating in vitro to in vivo exposures with physiologically based tissue dosimetry and tissue response models. (cdc.gov)
  • Histological, RT-qPCR, western blot analyses and steroid hormone measurements were performed on in vitro cultured mouse prepubertal testicular tissues and age-matched in vivo controls. (elifesciences.org)
  • Several fertility restoration approaches, whose aim is to mature cryopreserved tissues in vivo or in vitro in order to produce spermatozoa, are being developed. (elifesciences.org)
  • Experimental study of SAMMA's in vitro and in vivo properties. (nih.gov)
  • assessment of safety in vitro and in vivo. (nih.gov)
  • Based on result of this study, it is concluded that the best method for IVP (in vitro production) of cattle embryos is using percoll gradient with 500 ul per layer. (lipi.go.id)
  • 1993. Development of bovine embryos in vitro as affected by energy substrates. (lipi.go.id)
  • human embryos [2]. (who.int)
  • 11 ] developed the first time-lapse system for studying morphokinetics in human embryos. (ecerm.org)
  • 3 After washing, the sperm can be combined with ova from the female partner using in vitro fertilisation (IVF) techniques or direct injection of the sperm into a selected oocyte-intracytoplasmic sperm injection (ICSI). (bmj.com)
  • Although treatments, such as percutaneous epididymal sperm aspiration (PESA) and testicular sperm extraction (TESE) followed by intracytoplasmic spermatozoa injection (ICSI), are available for azoospermic patients, there is still need to improve the therapeutic approaches. (ijfs.ir)
  • Do female couples have to be married before having in vitro fertilisation treatment with the ROPA technique? (institutobernabeu.com)
  • If a couple wishes to undergo any alternative type of assisted reproduction technique ( in vitro fertilisation treatment with donor sperm, embryo adoption , IVF with dual donation , insemination and so on), they can adopt the child as their own and, by doing so, share motherhood. (institutobernabeu.com)
  • In order to be able to perform vitro fertilisation treatment using the ROPA method or, in other words, to achieve motherhood shared by two women (one provides the ova and the other receives the embryo that is generated and gestates), Spanish law 14/2006 on assisted human reproduction techniques indicates one essential requirement: the couple must be married. (institutobernabeu.com)
  • Assisted reproduction clinics in Spain have to request a marriage certificate from female couples choosing to have in vitro fertilisation with the ROPA method. (institutobernabeu.com)
  • In addition, viable and fertile mouse offspring have been obtained from in vitro produced spermatozoa by oocyte microinjection ( 3 , 4 ). (elifesciences.org)
  • Human development is a continuous process that begins when an oocyte (ovum) from a female is fertilized by a sperm (or spermatozoon) from a male. (americanrtl.org)
  • However, since the 1950s, various studies have shown translational activity within spermatozoa, particularly during their in vitro capacitation. (bvsalud.org)
  • A complete in vitro spermatogenesis has been obtained from mouse prepubertal testicular tissue, although with low efficiency. (elifesciences.org)
  • In vitro spermatogenesis could indeed be proposed to patients with testicular localization of residual tumor cells, for whom testicular tissue autografting is not indicated (about 30% of patients with acute leukemia). (elifesciences.org)
  • A decline in intratesticular testosterone levels has been highlighted in vitro in 35- to 49-day cultures of 5 d pp mouse testes, i.e. after the end of the first wave of spermatogenesis ( 9 ). (elifesciences.org)
  • Human testisderived cells (hTCs) are suitable candidates for the initiation of in-vitro spermatogenesis for these types of patients. (ijfs.ir)
  • As of 2004, several studies have shown that embryonic stem cells may restore the spermatogenesis and functional sperms in mouse and human, known as in vitro gametogenesis (IVG) (2-4). (ijfs.ir)
  • This scientifically designed non-spermicidal lubricant does not harm viability or motility of human sperm as compared to treatment with the optimum culture medium alone. (conceiveplus.com)
  • In vitro tests shows that the unique Ca2+ and Mg2+ containing lubricant is non-spermicidal and does not harm viability or motility of human sperm. (conceiveplus.com)
  • Falzone N, Huyser C, Fourie F, Toivo T, Leszczynski D, Franken D. In vitro effect of pulsed 900 MHz GSM radiation on mitochondrial membrane potential and motility of human spermatozoa. (naturalmedicinejournal.com)
  • In January 1990, a health department in the United States received a report of human immunodeficiency virus type 1 (HIV-1) infection in a woman who had been artificially inseminated with semen from her HIV-1-infected, hemophilic husband. (cdc.gov)
  • People infected with human immunodeficiency virus (HIV) are living longer and experiencing improved health. (bmj.com)
  • The risk of mother-to-child transmission of human immunodeficiency virus (HIV) during pregnancy, delivery, and breastfeeding is as high as 25-30% in the absence of treatment. (medscape.com)
  • ABSTRACT Objective: The primary objective of this study was to evaluate the performance of the novel calcium and magnesium containing non-spermicidal vaginal lubricant (Sasmar Conceive Plus®) using established in vitro assays. (conceiveplus.com)
  • METHODS The in vitro assays used in this study were according to literature protocols and followed the published WHO (World Health Organisation) criteria. (conceiveplus.com)
  • Following our previous animal studies performed on mice (9, 10), the aim of this study was to evaluate the viability and proliferation of the cells derived from human TESE samples, which were cultivated on a novel threedimensional (3D) nano-scaffold containing human serum albumin (HSA)/tri calcium phosphate nanoparticles (TCP NPs), as examined by MTT and H&E histological staining assays. (ijfs.ir)
  • From the early beginnings of in vitro fertili- motility and improves retention of sperm zation (IVF) it has been recognized that the motility in long-term incubation of both culture media supplemented with proteins fresh and cryopreserved, thawed human have a direct role in osmoregulation. (who.int)
  • Cryopreserved prepubertal testicular biopsies could theoretically be later matured in vitro to produce spermatozoa for assisted reproductive technology. (elifesciences.org)
  • This study reports useful information on the limits of the organotypic culture of neonatal mouse testes, which has been regarded as an experimental strategy that can be extended to humans in the clinical setting for the conservation and subsequent re-use of testicular tissue. (elifesciences.org)
  • The organotypic culture procedure, which preserves testicular tissue architecture, microenvironment and cell interactions, has been used successfully to obtain spermatozoa from fresh or frozen/thawed mouse prepubertal testicular tissues ( 3 - 6 ). (elifesciences.org)
  • Cells harvested from the testicular sperm extraction (TESE) samples of the azoospermic patients were cultured on the 3D porous scaffolds containing human serum albumin (HSA)/tri calcium phosphate nanoparticles (TCP NPs) for two weeks. (ijfs.ir)
  • Therefore, 3D HSA/TCP NPs scaffolds could be used for the reconstitu- tion of the artificial human somatic testicular niche for future applications in regenerative medicine for male infertility. (ijfs.ir)
  • TESE samples were taken after obtaining written informed consent from two non-obstructive azoospermic patients (with the ages of 27 and 36 years) who had rare immotile spermatozoa in testicular biopsies with complete spermatogenic arrest, unremarkable spermatogonia, normal Leydig cells, and normal serum hormones. (ijfs.ir)
  • see at cargnegiescience.edu from the Carnegie Stages of Early Human Embryonic Development , Stage 1. (americanrtl.org)
  • Spermatozoa are rendered dysfunctional by lipid peroxidation and altered membrane function, together with impaired metabolism, morphology, and motility [ 2 ]. (ijbs.com)
  • In October and December, fresh ejaculate was fractionated by centrifugation through a discontinuous density gradient of polyvinylpyrrolidone-coated silica particles (Percoll((R))**) to separate motile spermatozoa from other cells and seminal plasma. (cdc.gov)
  • The fraction containing motile spermatozoa was washed twice in buffer and introduced into the woman's uterus through a catheter. (cdc.gov)
  • Comparision of single and two layer Percoll sparation for selection of motile spermatozoa. (lipi.go.id)
  • By contrast, 5 weeks of RF-EME exposure adversely impacted the vitality and motility profiles of mature epididymal spermatozoa. (nature.com)
  • These spermatozoa also experienced increased mitochondrial generation of reactive oxygen species after 1 week of exposure, with elevated DNA oxidation and fragmentation across all exposure periods. (nature.com)
  • The potential for this mechanism of biophysical interaction provides the impetus for well-designed studies to ascertain the effect of RF-EME following whole-body irradiation regimens that more accurately mimic human exposure. (nature.com)
  • In both human and animal studies, exposure to radiofrequency radiation emitted from wireless devices is absorbed by the body and can harm the reproductive system. (ewg.org)
  • Exposure to radiofrequency radiation is associated in human studies with lower sperm concentration, viability, and motility. (ewg.org)
  • In vitro exposure of human lymphocytes to 900 MHz CW and GSM modulated radiofrequency: studies of proliferation, apoptosis and mitochondrial membrane potential med. (emf-portal.org)
  • Public health officials and others concerned with appropriate actions to take at hazardous waste sites may want information on levels of exposure associated with more subtle effects in humans or animals (LOAEL) or exposure levels below which no adverse effects (NOAELs) have been observed. (cdc.gov)
  • Estimates of exposure levels posing minimal risk to humans (Minimal Risk Levels or MRLs) have been made for di-n-butyl phthalate. (cdc.gov)
  • An MRL is defined as an estimate of daily human exposure to a substance that is likely to be without an appreciable risk of adverse effects (noncarcinogenic) over a specified duration of exposure. (cdc.gov)
  • As these kinds of health effects data become available and methods to assess levels of significant human exposure improve, these MRLs will be revised. (cdc.gov)
  • Molecular Human Reproduction , 8 (4), 326-332. (dundee.ac.uk)
  • The purpose of this review is to compare CDs to ERC, to describe ERC's relevance to human reproduction, and to clarify its assessment and clinical importance. (biomedcentral.com)
  • Sexual reproduction is common in eukaryotic organisms from yeasts to humans. (webmedcentral.com)
  • Human Reproduction Update. (edu.au)
  • Reactive oxygen species (ROS) play an important role in the human reproduction. (ijbs.com)
  • Last of all, we must take into account that sexual and reproduction rights are part of our human rights. (institutobernabeu.com)
  • Folic acid and human reproduction. (fertilevitamin.com)
  • Oxidative stress can damage the DNA of the spermatozoa and prevent them from fertilizing an egg. (mensfe.net)
  • Significant damage to human sperm from cell phone radiation, confirming studies that have been carried out in Australia, the US and Greece. (safertechsolutions.org)
  • To counteract oxidative damage, spermatozoa and seminal plasma have several mechanisms to neutralize free radicals. (scialert.net)
  • BACKGROUND Importance of Ca2+ and Mg2+ Ions Calcium and Magnesium ions are naturally present in human semen and cervical fluids. (conceiveplus.com)
  • Each mucus sample was divided into four portions collected into a capillary tube and kept either at room temperature or at 4°C, -20° C or in liquid nitrogen for 1 h, afterwards thawed at 37°C and used in the in vitro penetration device for 1 h. 56 semen samples of good quality were used, the same sample for all four types of the same cervical mucus. (tau.ac.il)
  • Abnormal spermatozoa are not fully mature, and when in the presence of white blood cells, they can produce harmful substances. (mensfe.net)
  • Excessive generation of ROS in semen, mainly by neutrophils but also by abnormal spermatozoa, could be linked with infertility. (ijbs.com)
  • The excessive generation of oxygen free radicals in the form of ROS by abnormal spermatozoa and by contaminating leukocytes has been identified as one of the few defined etiologies for male infertility. (scialert.net)
  • To counteract the negative effects of excess ROS, spermatozoa and seminal plasma have several mechanisms to neutralize free radicals. (scialert.net)
  • control (containing TALP + spermatozoa) and the experimental [treated / untre. (webmedcentral.com)
  • Quantitative comparison to experimental data on human sperm exhibiting typical penetrative, activated and hyperactivated beats shows a good fit to the time-average absolute curvature profile along the flagellum, providing evidence that the model is capable of providing a framework for quantitative interpretation of imaging data. (bvsalud.org)
  • The CDs of mature non-human mammalian sperm are found at the distal end of the midpiece. (biomedcentral.com)
  • Mature human spermatozoa are similar to that of other mammals in possessing a CD at the midpiece. (biomedcentral.com)
  • Here, we investigate the presence and activity of the two types of ribosomes in mature human spermatozoa. (bvsalud.org)
  • Beside mitotic and meiotic divisions of SSCs for the production of mature spermatozoa, there are different significant factors that play roles in this process. (ijfs.ir)
  • Semen samples from 13 subjects were incubated in human tubal fluid media (HTF) controls and 10% (vol/vol) of Pre-Seed, FemGlide, Astroglide, and Replens lubricants. (nih.gov)
  • In mammals, the sex of the offspring is determined by the sperm cell: a spermatozoon bearing an X chromosome will lead to a female (XX) offspring, while one bearing a Y chromosome will lead to a male (XY) offspring. (wikipedia.org)
  • We speculate that keeping the cell phone in a trouser pocket in talk mode may negatively affect spermatozoa and impair male fertility. (naturalmedicinejournal.com)
  • Lastly, the phone and the male reproductive organs are separated by multiple tissue layers, so to extrapolate the effects seen in vitro to "real-life" conditions requires further studies, which are currently under way. (naturalmedicinejournal.com)
  • Recent studies have shown cytoplasmic droplets to be normal morphological occurrences in human male spermatozoa. (biomedcentral.com)
  • Azoospermia is defined as the lack of spermatozoa in semen, and it is one of the challenging disorders in male infertility. (ijfs.ir)
  • The WHO/International Agency for Research on Cancer (IARC) has classified radiofrequency electromagnetic fields as possibly carcinogenic to humans (Group 2B), based on an increased risk for glioma, a malignant type of brain cancer, associated with wireless phone use. (slideshare.net)
  • Effects of radiofrequency electromagnetic waves (RF-EMW) from cellular phones on human ejaculated semen: an in vitro pilot study. (naturalmedicinejournal.com)
  • The use of the stain exclusion assay and the hypoosmotic swelling test in determining the viability of human sperm was compared in sperm samples obtained from a group of 11 healthy males. (cdc.gov)
  • Hypogonadism is defined as testosterone deficiency with associated symptoms or signs, deficiency of spermatozoa production, or both. (msdmanuals.com)
  • Some syndromes of hypogonadism (eg, cryptorchidism, some systemic disorders) affect spermatozoon production more than testosterone levels. (msdmanuals.com)
  • It can be found in human serum, follicular fluid, and Among the essential components that affects the plasma [11-13]. (who.int)
  • 17 mm in diameter, 10 000 units of human support was continued until 10 weeks gesta- chorionic gonadotropin (Organon, Holland) tion. (who.int)
  • The title of the Opinion is 'Preliminary Opinion on Possible Effects of Electromagnetic Fields (EMF) on Human Health" (emphasis added). (bioinitiative.org)
  • Indeed, it has been shown that spermatozoa provide a sensitive model to study the specific physical and chemical responses to RF-EME 15 . (nature.com)
  • Human sperm genetics has been associated with human evolution, per a 2020 study. (wikipedia.org)
  • 2004). Therefore we concluded from this study that the proposition based on the findings in studies in non-human subjects that short-term use of agent to treat malaria has a significant anti-spermatogenic and anti-fertility or contraception. (researchgate.net)
  • To study the nonthermal effects of radiofrequency fields on human immune cells ( peripheral blood mononuclear cells ) exposed to a Global System for Mobile Communication ( GSM ) signal generated by a commercial cellular phone and by a sinusoidal non- modulated signal ( continuous wave ). (emf-portal.org)
  • This study was limited by the fact that it is a pilot study-a small number of participants and nonrandomized to a group of RF EMW‒unexposed men (the in vitro samples of semen were controlled. (naturalmedicinejournal.com)
  • Transgenic technology is a most important tool for researching all kinds of disease in humans and animals, and for understanding crucial problems in biology," said Anil Chandrashekran, Ph.D., study author from the Department of Veterinary Clinical Sciences at The Royal Veterinary College in North Mimms, United Kingdom. (science20.com)
  • Prospective, comparative, in vitro study. (nih.gov)
  • In human, as well as in veterinary medicine, several dietary protocols have been developed to relieve poor sperm concentration and function. (mdpi.com)
  • By targeting ribosomal RNAs and proteins, we show that both types of ribosomes are localized in the midpiece as well as in the neck and the base of the head of the spermatozoa. (bvsalud.org)
  • However, in vitro sperm production still remains a rare event. (elifesciences.org)
  • Oxygen free radicals generated by spermatozoa may be involved in the production of spermicidal cytotoxic end products [ 4 ]. (ijbs.com)
  • Therefore, this review will attempt to discuss the relevance of free radicals and antioxidants in spermatozoa production and function. (scialert.net)
  • In this review, we summarised human and animal brucellosis, prevalence of infections in Nigeria, and economic impacts on production. (bvsalud.org)
  • This article reviews the morphologic, physiologic, functional, and pathologic aspects of the human oviduct. (medscape.com)
  • Effects of quality and development stage on the survival of FIV-derived bovine blastocyst cultured in vitro after freezing and thawing. (lipi.go.id)
  • We have investigated the effects of tyrosine kinase inhibition on [Ca2+]i responses in large numbers of individual human spermatozoa. (dundee.ac.uk)
  • LOAELs or NOAELs should also help in determining whether or not the effects vary with dose and/or duration, and place into perspective the possible significance of these effects to human health. (cdc.gov)
  • Mandelic acid condensation polymer is not cytotoxic toward lactobacilli, microbial host cells, and spermatozoa. (nih.gov)
  • Semen has an alkaline nature and the spermatozoa do not reach full motility (hypermotility) until they reach the vagina, where the alkaline pH is neutralized by acidic vaginal fluids. (wikipedia.org)
  • To evaluate the effect of vaginal lubricants Pre-Seed, FemGlide, Astroglide, and Replens on human sperm motility and chromatin integrity. (nih.gov)
  • and human spermatogenic stem cell culture to treat azoospermia, and to preserve fertility in pre-pubertal boys undergoing cancer treatment. (infertile.com)
  • When pseudotyped lentiviral vectors encoding green fluorescent protein (GFP) were incubated with mouse spermatozoa, these sperm were highly successful in producing transgenics. (science20.com)
  • Separation of human X and Y bearing sperm using Percoll density gradient centrifugation. (lipi.go.id)
  • Because of its cause we have not made use of this medium characteristic high abundance in the female before in our department, we aimed to com- reproductive tract, albumin has traditionally pare the IVF implantation and pregnancy served as the main macromolecule in most rate by using hyaluronic acid and albumin culture media used for in vitro growth of as transfer medium. (who.int)
  • After 4 hours culture, spermatozoa were analyzed for percent DNA fragmentation index with use of the acridine orange-based sperm chromatin structure assay. (nih.gov)
  • Moreover, in parallel, several groups have demonstrated the pluripotency of germ-line stem cells (GSCs) following SSCs culture in rodents, though, there is a debate about pluripotency of GSCs in primates and humans (5). (ijfs.ir)
  • Pro- spermatozoa [13] teins also serve as a source of energy and Because there have been relatively few as reservoirs for the release of hormones, human studies with hyaluronic acid and be- vitamins and minerals [1]. (who.int)
  • It is a familiar element of mammalian spermatozoa, though many studies in the past have focused primarily on CDs in non-human species. (biomedcentral.com)