Human skin cell. Medical search. Frequent questions

The models tested included melanoma cells, neonatal melanocytes and keratinocytes, and immortalized HaCaT keratinocytes. (biologists.com)
Keratinocytes and fibroblasts of epidermal and dermal layers of the skin play prominent roles in this process. (indexindex.com)
Human epithelial cells induce human melanocyte growth in vitro but only skin keratinocytes regulate its proper differentiation in the absence of dermis. (unimore.it)
Human keratinocytes isolated from a skin biopsy and cultured in vitro reconstitute a stratified squamous epithelium suitable for grafting on burned patients. (unimore.it)
b) melanocyte growth is not autonomous but requires close keratinocyte contact and is regulated to maintain a physiological melanocytes/keratinocytes ratiol and (c) pure skin keratinocytes, but not oral epithelial cells, have all the information required for the proper physiological location and differentiation of melanocytes in the epidermis. (unimore.it)
Psoriatic skin is marked by increased proliferation of keratinocytes the major cell of the outermost layer of skin resulting in a thickening of the epidermis. (bio2009.org)
Massive ex vivo expansion of epidermal cells (called keratinocytes) is needed for the production of grafts. (cea.fr)
It is performed using a skin sample from the patient that contains adult keratinocytes and a minority population of keratinocyte stem cells. (cea.fr)
In the future, keratinocytes produced from pluripotent stem cells could be an alternative to adult stem cells in certain reconstructed tissue bio-engineering applications. (cea.fr)
One of the difficulties encountered in this area is that fact that the keratinocytes obtained do not have all the functions of adult stem cells. (cea.fr)
The study has shown that manipulation of KLF4 expression is also suitable for these cells, as reducing its expression in keratinocytes derived from embryonic stem cells (ESC) improves their proliferation capacity and their ability to reconstruct skin. (cea.fr)
1999. Response of normal human keratinocytes to sulfur mustard (HD): Cytokine release using a non-enzymatic detachment procedure. (cdc.gov)
Whereas the expression of syndecan-4 and glypican in intact skin was mostly in the pericellular regions of keratinocytes, the epidermal cells from the wound edge contained mostly intracellular PGs. (lu.se)
It is an autoimmune skin disorder characterized by the loss of intercellular adhesion of keratinocytes in the upper parts of the epidermis (acantholysis), resulting in the formation of superficial blisters. (medscape.com)

Protein expression Collagen peptide Human skin fibroblasts cells UVA. (ajol.info)
In this study, we reported the cloning and over expression of a gene coding for human collagen peptide (CP6) in Escherichia coli and investigated the protective effects of CP6 on UVA-irradiated human skin fibroblasts cells. (ajol.info)
Also, we performed assays with 3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyltetrazolium bromide (MTT), Annexin V-fluorescein isothiocyanate/propidine iodide (Annexin V-FITC/PI) and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) methods to investigate the cytoprotective effects of CP6 on the proliferation of UVA-damaged human skin fibroblasts cells. (ajol.info)
Radiation dosages (5 J/cm 2 ) significantly decreased the proliferation activities of human skin fibroblasts cells (HSF). (ajol.info)
UVA could damage the human skin fibroblasts cell in vitro . (ajol.info)
In cell proliferation assays, asiaticoside induced an increase in the number of normal human dermal fibroblasts. (indexindex.com)
Here, we provide evidence that exposure of human skin fibroblasts to a Radio Electric Asymmetric Conveyer (REAC), an innovative device delivering radio electric conveyed fields at a radiofrequency of 2.4 GHz, afforded remarkable commitment toward cardiac, neuronal, and skeletal muscle lineages. (nih.gov)
Comparing Effects of Short-and Long-Term Exposure of Cigarette Smoke Extract on Human Vocal Fold Fibroblasts. (invivogen.com)
My Ph.D. studies have focused on developing ways to generate human astrocytes from pluripotent stem cells and fibroblasts for modeling diseases affecting the human brain. (lu.se)

Compared to studying individual cells in a petri dish, organoids allow researchers to study cells in more lifelike environs, as the clumps of tissue will form different types of structures akin to real organs in the body. (alleninstitute.org)
Through the course of that project, which used mouse stem cells rather than human cells, he and his colleagues managed to generate skin tissue as a by-product. (alleninstitute.org)
Ironically, the Allen Cell Collection is built from stem cells first generated at the Gladstone Institutes from an adult donor's skin cells - although that doesn't mean the stem cells are more similar to skin than they are to other types of tissue. (alleninstitute.org)
Such treatments include allogeneic and xenogeneic transplants and skin substitutes such as tissue-engineered skin, cultured cells, and stem cells. (biomedcentral.com)
The aim of this paper is to discuss the general overview of the quality assurance and quality control implemented in the manufacturing of cell and tissue product, with emphasis on our experience in the manufacturing of MyDerm ® , an autologous bilayered human skin substitute. (biomedcentral.com)
Manufacturing MyDerm ® requires multiple high-risk open manipulation steps, such as tissue processing, cell culture expansion, and skin construct formation. (biomedcentral.com)
In recent years, the development in cell therapy and stem cell research has led scientist to engage in the production of cells and tissue product that is of clinical grade. (biomedcentral.com)
This partial amino acid sequence corresponded to the sequence of human heart chymase, a proteinase isolated from heart tissue with immunologic and hydrolytic properties similar to skin chymase. (duke.edu)
Circulating memory T cells can be divided into tissue-specific subsets, which traffic through distinct tissue compartments during physiologic immune surveillance, based on their expression of adhesion molecules and chemokine receptors. (biomedcentral.com)
Consequently, circulating T cells can be divided into tissue specific subsets, each of which have the ability to traffic through certain tissue compartments, but which are excluded from others. (biomedcentral.com)
The in vitro permeation behavior of RocBr from its lotion formulation was quantified with Strat-M ® synthetic biomimetic membrane and EpiDerm™ 3D human skin tissue. (arizona.edu)
When a tissue experiences inflammation, its cells remember. (scitechdaily.com)
Scientists have managed to create synthetic human embryo models without using egg, sperm or womb, in a feat that could impact research on fertility, tissue growth and drug testing, as well as improve science's understanding of the first weeks of embryonic development. (israel21c.org)
A CEA-Jacob team has just published a paper in which it demonstrates the central role of the transcription factor KLF4 in regulating the proliferation of epidermal stem cells and their ability to regenerate this tissue. (cea.fr)
Human skin completely renews itself every month thanks to the presence of stem cells in the deepest layer, which generate all the upper layers of this tissue. (cea.fr)
The discoveries of a French research team from the CEA, INSERM and the University of Paris, produced in collaboration with I-Stem, the AFM-Téléthon laboratory, and the University of Évry, opens perspectives for regenerative cutaneous medicine, in particular for the bio-engineering of skin grafts for tissue reconstruction. (cea.fr)
a germicide that is used on skin or living tissue for the purpose of inhibiting or destroying microorganisms. (cdc.gov)
It is very difficult to develop therapeutics for central nervous system disorders owing to several challenges in studying the human brain due to its exceptionally high complexity that is not present in other animal species, and inaccessibility to tissue samples from patients and healthy individuals. (lu.se)
In May 2010, the Sixty-third World Health Assembly adopted resolution WHA63.22,1 in which it endorsed the updated WHO Guiding Principles on Human Cell, Tissue and Organ Transplantation and provided strategic directions to support progress in human organ, tissue and cell donation with the aim of maximizing the benefits of transplantation, meeting the needs of recipients, protecting donors and ensuring the dignity of all involved. (who.int)
The benefits of human tissue transplantation can be seen in both children and adults, including in survival rates following severe burn trauma, recovery of movement, closure of chronic wounds, rehabilitation of heart function and restoration of sight. (who.int)

The research team had set up a system to grow human skin in the lab, starting with induced pluripotent stem cells, naïve adult human cells with the potential to give rise to many other kinds of tissues. (alleninstitute.org)
Investigators took skin cells biopsied from volunteers with an inherited form of glaucoma and from volunteers without the disease and genetically reprogrammed them to become pluripotent stem cells. (siasat.com)
The researchers started out with human pluripotent stem cells, which can differentiate into various cell types. (israel21c.org)
The researchers reprogrammed the pluripotent stem cells to an earlier (naïve) stage corresponding to day 7 of a natural human embryo, around the time it implants itself in the womb. (israel21c.org)
By using a new approach, we developed a rapid and efficient method to generate functional and mature astrocytes from human pluripotent stem cells through overexpression of certain transcription factors that control the development of astrocytes. (lu.se)

The researchers showed they could convert human embryonic stem cells to neurons by infecting them with a virus that expressed the same proteins used in the study. (stanforddaily.com)
This treatment, nicknamed "BAM" after an acronym of the three proteins, converted the embryonic stem cells into functional neurons within six days. (stanforddaily.com)
In a feat of bioengineering, scientists take adult skin cells and then essentially reprogram them to turn them into embryonic stem cells that can be grown into all manner of cells. (naturalnews.com)
A paper appearing in the online version of Cell this week describes the successful reprogramming of human skin cells into embryonic stem cells. (bigthink.com)
Scientists at Oregon Health & Science University and the Oregon National Primate Research Center (ONPRC) have successfully reprogrammed human skin cells to become embryonic stem cells capable of transforming into any other cell type in the body. (ohsu.edu)
The research breakthrough, led by Shoukhrat Mitalipov, Ph.D. , a senior scientist at ONPRC, follows previous success in transforming monkey skin cells into embryonic stem cells in 2007. (ohsu.edu)
A thorough examination of the stem cells derived through this technique demonstrated their ability to convert just like normal embryonic stem cells, into several different cell types, including nerve cells, liver cells and heart cells. (ohsu.edu)
These embryo-like structures went on to develop for eight days outside the womb, reaching a stage equivalent to day 14 in natural human embryonic development. (israel21c.org)

Scholars@Duke publication: Determination of the primary structures of human skin chymase and cathepsin G from cutaneous mast cells of urticaria pigmentosa lesions. (duke.edu)
The deduced amino acid sequence between the primers was identical to that reported for neutrophil cathepsin G, indicating that the protein of cutaneous mast cells previously shown to be immunologically cross-reactive with neutrophil cathepsin G has a comparable amino acid sequence. (duke.edu)
For instance, studies in mice have demonstrated that CD4+ T cells activated in cutaneous lymph nodes upregulate trafficking determinants specific for the skin, such as P-selectin ligand, while T cells responding to antigen in intestinal lymph nodes express high levels the gut-associated adhesion molecule integrin α4β7 and acquire responsiveness to the intestinal CC-chemokine ligand CCL25 [ 2 ]. (biomedcentral.com)
Cutaneous squamous cell carcinoma (cSCC) is the second-most common type of non-melanoma skin cancer and is linked to long-term exposure to ultraviolet (UV) radiation from the sun. (arizona.edu)
This work has been extended to other types of cells of interest for cutaneous cell therapy. (cea.fr)
These defects include impaired cutaneous responses to recall antigens, decreased ratio of circulating T-helper cells to suppressor cells, impaired lymphocyte proliferative responses to mitogen, impaired production of interferon in lymphocytes, and reduced natural killer cell activity. (medscape.com)
The purpose of this article is to integrate renal and cutaneous aspects of disease as well as highlight some important, although frequently underappreciated, clinical or laboratory findings that ally renal and skin diseases. (medscape.com)
Natural History of Incident and Persistent Cutaneous Human Papillomavirus and Human Polyomavirus Infections. (who.int)

When in vitro fertilization was first developed, a lot of people were disturbed by the idea of creating a baby outside of the human baby - and many still are. (naturalnews.com)
Scientists are expected to have the ability to create babies from human skin cells within the next two decades in a process known as in vitro gametogenesis, or IVG. (naturalnews.com)
Using in vitro systems, we observed the effects of asiaticoside on normal human skin cell behaviors related to healing. (indexindex.com)
To investigate the mechanisms of anaphylactoid reactions to radiocontrast media, in vitro mediator release induced by three iodinated contrast agents was examined using peripheral blood basophils and mast cells purified from human lung parenchyma, heart, and skin tissues. (johnshopkins.edu)
Peptide fractions extracted from Flathead by-products were evaluated for in vitro free radical scavenging and cancer cell cytotoxic activities. (springer.com)
In vitro cleavage of diisocyanate -glutathione conjugates by human gamma-glutamyl transpeptidase-1. (cdc.gov)

This study establishes the primary structure of human skin chymase and provides further evidence for the presence of a cathepsin G-like proteinase within human mast cells. (duke.edu)
UP is a disease characterized by skin lesions containing high numbers of mast cells. (duke.edu)
These studies provide further evidence that human skin mast cells contain two different chymotrypsin-like proteinases. (duke.edu)
Three iodinated contrast agents, sodium and meglumine salts of ioxaglic acid, sodium and meglumine salts of ioxithalamic acid, and ioversol, were incubated with basophils purified from peripheral blood and human mast cells isolated and purified from different anatomical sites. (johnshopkins.edu)
All three induced the release of preformed mediators (histamine and tryptase) from human lung, but not from skin mast cells. (johnshopkins.edu)
They also induced histamine and tryptase release from human heart mast cells. (johnshopkins.edu)
Cross-linking of IgE by anti-IgE induced the release of leukotriene C 4 or prostaglandin D 2 from human basophils or mast cells. (johnshopkins.edu)
Mannitol, an osmotic stimulus, induced the release of histamine from human basophils, but to a lesser extent from mast cells. (johnshopkins.edu)
These results show that different contrast media can differ in their ability to release mediators from enriched preparations of human basophils and mast cells. (johnshopkins.edu)
The three contrast agents examined act on basophils and mast cells as incomplete secretagogues, causing the release of preformed mediators, but not the de novo synthesis of chemical mediators. (johnshopkins.edu)

It had layers of dermis and epidermis, just like real skin, and tiny mouse hair follicles. (alleninstitute.org)
The outer layer of skin, the epidermis, is chock full of desmosomes. (alleninstitute.org)
These glowing structures allowed the researchers to better track the formation of different skin layers in the organoids - they could see the epidermis developing in real-time as the fluorescent signal got brighter under the microscope. (alleninstitute.org)
Inflamed mouse stem cells located in the basal layer (red) of the epidermis and FOS (green), a near-universal stress response factor essential to inflammatory memory. (scitechdaily.com)
Here, we evaluated dermal toxicity of ionic liquids using human keratinocyte and fibroblast cell line, 3D reconstructed human epidermis, and full-thickness model to investigate underlying mechanisms. (elsevierpure.com)
In 3D reconstructed human epidermis and full-thickness model, a single application of [TFSI] induced toxicity although it was minimal and largely limited to epidermal layer. (elsevierpure.com)
The Nikolsky sign is the finding that physical trauma can shear the pathologic epidermis of the skin of patients with pemphigus foliaceus, resulting in clinical lesions. (medscape.com)

Scientists from the Oregon Health & Science University and the Oregon National Primate Research Center figured out that the fragility of human egg cells - into which a nucleus from another human cell, in this case skin, is transferred and begins to produce stem cells - required a different approach. (bigthink.com)
Furthermore, the comparative fragility of human cells as noted during this study, is a significant factor that would likely prevent the development of clones. (ohsu.edu)

GENERAL INFORMATION Human A375 Skin Melanoma Cells were isolated from a female patient with malignant skin melanoma. (ubpone.com)
Here we investigated the effect Cisplatin (CDDP), one of the most potent chemotherapeutic agent used to treat tumors, in association with fasting in wild type and mutated BRAF(V600E) melanoma cell lines. (unisalento.it)
In addition, we show that autophagy is not involved in the enhanced sensitivity of melanoma cells to combined CDDP/EBSS-induced apoptosis. (unisalento.it)
Another type of skin cancer, melanoma , is more dangerous but less common. (medlineplus.gov)

In the present study, the venoms from five sea anemones (Heteractis crispa, Heteractis magnifica, Heteractis malu, Cryptodendrum adhaesivum and Entacmaea quadricolor) were obtained by the milking technique, and the potential of these venoms to kill cancer cells was tested on three cell lines (A549 lung cancer, T47D breast cancer and A431 skin cancer). (scielo.br)
The results indicate that the sea anemone venom is cytotoxic to human cancer cells. (scielo.br)
sea anemone venoms, cytolysin, cancer cell line. (scielo.br)
Genetic studies in mice have shown that mutation of the CK2 phospho-acceptor site in p53 increases UV-induced skin cancer formation,(11) highlighting an unexpected role for p53 phosphorylation in mediating p53-dependent tumor suppression. (dundee.ac.uk)
3 kDa inhibited the growth of HT-29 colon cancer cells up to 91.04 %, although the activity was found to be non-selective. (springer.com)
In: Mukhtar H (ed) Skin cancer: mechanisms and human relevance. (springer.com)
The DeGroote facility is shared with the Centre for Function Genomics, Centre for Gene Therapeutics, Institute for Cancer and Stem Cell Biology Research, Robert E. Fitzhenry Vector Laboratory, Centre for Asthma and Allergy Research (Allergen) and North American Headquarters for West Nile studies, as well as the Bachelor of Health Sciences undergraduate program. (wikipedia.org)
Skin cancer is the most common form of cancer in the United States. (medlineplus.gov)
The two most common types are basal cell cancer and squamous cell cancer. (medlineplus.gov)
If not treated, some types of skin cancer cells can spread to other tissues and organs. (medlineplus.gov)
PDT uses a drug and a type of laser light to kill cancer cells. (medlineplus.gov)
What Can I Do to Reduce My Risk of Skin Cancer? (medlineplus.gov)
What's New in Basal and Squamous Cell Skin Cancer Research? (medlineplus.gov)

While, the exposure to 2-DG further enhanced the apoptotic rate observed in SK Mel 28 cells upon treatment with both CDDP and EBSS. (unisalento.it)
Research has also shown that following a significant exposure of the skin to the ultraviolet light, the amounts of these antioxidants present in the skin are significantly depleted. (mattek.com)
To determine the safety of the fermented extracts, their cytotoxicity was analyzed by measuring the cell viability of CCD-966SK and human epidermal melanocytes (HEMn) after exposure. (hindawi.com)
Moreover, extrinsic factors such as exposure to UV radiation lead to the activation of collagenase, elastase, and tyrosinase, thus resulting in skin aging, wrinkle formation and melanin production [ 7 , 8 ]. (hindawi.com)
However, little is known about toxicity of ionic liquids on the skin, a major exposure portal to toxic substances. (elsevierpure.com)
The presence of bioactive peptides during H 2 O 2 exposure increased viability of T4056 normal cells. (springer.com)
However, potency deci- exposure to the chemical as well as an under- sions regarding skin sensitizers are based on pre- standing of its relative allergenic potency. (cdc.gov)

Stem cell therapies have long been seen as a holy grail treatment for many common diseases including multiple sclerosis and Parkinson's disease, yet many have questioned the ethics of using developing human embryos for this purpose. (bigthink.com)
associated with skin-homing) as well as the expression of integrin α4β7 and CCR9 (associated with gut-homing) was analyzed on CD4+ memory T cells in CSF from individuals with non-inflammatory neurological diseases using flow cytometry. (biomedcentral.com)
Diseases or conditions that might be treated through stem cell therapy include Parkinson's disease, multiple sclerosis, cardiac disease and spinal cord injuries. (ohsu.edu)
This is a remarkable accomplishment by the Mitalipov lab that will fuel the development of stem cell therapies to combat several diseases and conditions for which there are currently no treatments or cures," said Dr. Dan Dorsa, Ph.D. , OHSU Vice President for Research. (ohsu.edu)
This achievement has significant implications for understanding pregnancy development, studying pregnancy-related diseases, and advancing cell therapies. (austfhu.org.au)
2. University of Évry, University of Paris-Saclay, INSERM U861, Institute for Stem Cell Therapy and Exploration of Monogenic Diseases (I-Stem), 91100 Corbeil Essonne, France. (cea.fr)
3. Centre for the Study of Stem Cells (CECS), Institute for Stem Cell Therapy and Exploration of Monogenic Diseases (I Stem), 91100 Corbeil Essonne, France. (cea.fr)
Instead, by developing models in the dish with high biological relevance and predictive value for the human brain, we can accelerate our knowledge about what occurs during diseases affecting humans. (lu.se)
We focused on one of the main cell types found in our brains, the astrocyte, which over the last decade has emerged as an important contributor to a wide range of neurological diseases. (lu.se)
The transplantation of human tissues, organs or cells is an established form of treatment that has been acknowledged as the best and very often only life-saving therapy for several serious and life-threatening congenital, inherited and acquired diseases and injuries. (who.int)
Haematopoietic stem cell transplants have been performed in more than 1 500 000 patients (both autologous and allogeneic) to date.1 Although haematological cancers remain the main indication, haematopoietic stem cell transplants are increasingly considered in the treatment of non-malignant disorders and genetic diseases such as haemoglobinopathies (sickle cell anaemia, thalassaemia) that can benefit greatly from this type of transplant. (who.int)

The A549 cell line was the most sensitive of the cell lines tested with a significant reduction in viability observed at 40 µg/mL. (scielo.br)
Viability of cultured primary human skin cells treated with HDI monomer and HDI isocyanurate. (cdc.gov)

The differentiation of naïve T cells into an activated memory phenotype is characterized by an extensive change in the expression of trafficking determinants, resulting in the acquisition of homing receptors that enable the cells to migrate from the circulation into peripheral tissues. (biomedcentral.com)
These cells take up residence in tissues during defined developmental windows, when they may demonstrate distinct phenotypes and functions. (bvsalud.org)
While the development of xenografts (products of animal origin) and bioengineered alternatives is advancing, to date they cannot fully mimic or replace all human-sourced tissues on an equivalent basis. (who.int)
Thus, the availability of and access to human tissues for transplantation remains essential. (who.int)
In June 2018, the Secretariat established the WHO Task Force on Donation and Transplantation of Human Organs and Tissues as an advisory group composed of experts from all WHO regions. (who.int)

DENV-infected LCs efficiently transmitted DENV to target cells such as dendritic cells (DCs). (nih.gov)
This is happening in natural killer cells, T cells, dendritic cells from human skin, and epidermal stem cells in mice," says Samantha B. Larsen, a former graduate student in the laboratory of Elaine Fuchs at The Rockefeller University. (scitechdaily.com)

The hair-sprouting skin organoids also developed melanocytes (pink), which are cells that produce the skin and hair's pigment, melanin. (alleninstitute.org)

With the addition of four proteins, adult human skin cells can be transformed into neurons over a month-long period. (stanforddaily.com)
it triggered the skin cells' transformation into functional neurons within about four to five weeks. (stanforddaily.com)
The cells expressed electrical activity characteristic of neurons and even integrated and interacted with mouse neurons on a laboratory dish. (stanforddaily.com)
While they found that approximately 20 percent of mouse skin cells transform directly into functional neurons, under current culture conditions only about two to four percent of human skin cells do the same. (stanforddaily.com)
New York: Using stem cells derived from human skin cells, US researchers have revealed that these can be turned into retinal ganglion cells (RGCs) - the neurons that conduct visual information from the eye to the brain - to prevent or cure glaucoma. (siasat.com)
Although, there is still an incomplete understanding of how the teamwork between astrocytes, neurons, and other cells in the brain is carried out and what consequences occur when astrocytes stop working properly. (lu.se)

1998. Protection of human upper respiratory tract cell lines against sulphur mustard toxicity by hexamethylenetetramine (HMT). (cdc.gov)
The toxicity information for some of the health effects listed above may be categorical in nature (presence/absence of genotoxicity or skin irritation, for example) while other outcomes are expressed through quantitative information and/or potency data. (cdc.gov)
At that time, data were insufficient to assess the efficacy of ZDV as a prophylactic agent in humans or the toxicity of this drug in persons not infected with HIV. (cdc.gov)

We determine a comprehensive map of lineage-specific lncRNAs in human dermal lymphatic and blood vascular endothelial cells (LECs and BECs), combining RNA-Seq and CAGE-Seq. (nature.com)
Thereafter, a distinct subpopulation of endothelial cells lining the cardinal vein starts differentiating by expressing the TF PROX1, the master regulator of lymphatic endothelial cell (LEC) identity, via the TFs SOX18 and COUPTFII. (nature.com)

Melanin is the black pigment in hair and skin and is essential for protecting human skin against radiation. (hindawi.com)
Vitamin E has been proven to have antioxidant and moisturizing properties in the skin and can protect against the damage of UVB radiation, with emphasis on the reduction of acute erythema and photoaging. (intechopen.com)
Clinical studies of the skin of patients with xeroderma pigmentosum indicate prominent depletion of Langerhans cells induced by UV radiation. (medscape.com)

By not using actual embryos, scientists at two Oregon-based institutions sidestepped one of the largest issues surrounding stem cell technology. (bigthink.com)
In the laboratory of Prof. Jacob Hanna at the Weizmann Institute of Science , researchers created complete models of human embryos from stem cells cultured in a lab grew them up to day 14. (israel21c.org)

The findings, reported yesterday in Nature, suggest a process that doesn't require the reprogrammed adult somatic cells called induced pluripotent stem (iPS) cells. (stanforddaily.com)
The technique used by Drs. Mitalipov, Paula Amato, M.D. , and their colleagues in OHSU's Division of Reproductive Endocrinology and Infertility, Department of Obstetrics & Gynecology, is a variation of a commonly used method called somatic cell nuclear transfer, or SCNT. (ohsu.edu)
Several years of monkey studies that utilize somatic cell nuclear transfer have never successfully produced monkey clones. (ohsu.edu)
Somatic cells can be directly reprogrammed to alternative differentiated fates without first becoming stem/progenitor cells. (nih.gov)
Our results show for the first time the feasibility of using a physical stimulus to afford the expression of pluripotentiality in human adult somatic cells up to the attainment of three major target lineages for regenerative medicine. (nih.gov)

Pinning proteins to its genetic material at the height of inflammation, the cells bookmark where they left off in their last tussle. (scitechdaily.com)

We have used a human epidermal ex vivo infection model as well as isolated LCs to investigate infection by DENV. (nih.gov)

and even, one day, as the basis for better skin grafts for burn victims or other surgical uses. (alleninstitute.org)
KLF4 is therefore a new molecular target for preserving the functionality of stem cells and making progress in the bio-engineering of skin grafts. (cea.fr)

For much of its history psoriasis was believed to be solely a disorder of the skin characterised by aberrant keratinocyte activity. (bio2009.org)

These are able to differentiate into any cell type in the body. (siasat.com)
During development, the blood vascular system arises from endothelial cell progenitors that differentiate from mesodermal cells, mostly through the expression of the transcription factor (TF) ETV2. (nature.com)
Activation of the VEGFA/VEGFR2 signaling and expression of blood vascular endothelial cell (BEC) markers, such as NRP1 and EphrinB2, further differentiate these precursor cells into BECs, which then form the hierarchical network of blood vessels 4 . (nature.com)

He expressed hope that one day the cells could be used for human therapies. (stanforddaily.com)
It is believed that stem cell therapies hold the promise of replacing cells damaged through injury or illness. (ohsu.edu)
The presence of a large number of immune cells in psoriatic skin suggested that they play a part in the disease and the discovery that therapies targeting the immune system were effective for the treatment of psoriasis further highlighted its importance. (bio2009.org)

Recent genetic studies have identified a range of potential molecules relating to NK cell biology that are known to be important in psoriasis. (bio2009.org)
Development: For advances in developmental biology and stem cells. (lu.se)

Here, we utilized mass and flow cytometry to elucidate early features of human skin immunity . (bvsalud.org)
Now, a new study in Cell Stem Cell describes the mechanism behind inflammatory memory, also commonly referred to as trained immunity, and suggests that the phenomenon may be universal across diverse cell types. (scitechdaily.com)
When thinking about our immune system, we default to specific immunity-that cadre of T cells and B cells trained, by experience or vaccination, to remember the specific contours of the last pathogen that broke into our bodies. (scitechdaily.com)
But there's a less specific strategy available to many cells, known as trained immunity. (scitechdaily.com)
Trained immunity allows cells to respond to entirely new threats by drawing on general memories of inflammation. (scitechdaily.com)
Various other defects in cell-mediated immunity have been reported in xeroderma pigmentosum. (medscape.com)

Their latest study, published in the prestigious journal Nature Communications, reveals a remarkable breakthrough in converting skin samples into functional human placenta cells . (austfhu.org.au)
1. A single functional stem cell is able to regenerate the skin throughout a person's life. (cea.fr)

Global burden of HPV-attributable squamous cell carcinoma of the anus in 2020, according to sex and HIV status: A worldwide analysis. (who.int)

Although preventing blood exposures is the primary means of preventing occupationally acquired human immunodeficiency virus (HIV) infection, appropriate postexposure management is an important element of workplace safety. (cdc.gov)
Infectious causes of glomerulonephritis included streptococcal infections, human immunodeficiency virus (HIV) infection, and hepatitis viral infections, both hepatitis C (HCV) and hepatitis B (HBV). (medscape.com)

Matrix metalloproteinase 1 overexpression has been shown to occur in both aging of skin and carcinogenesis. (medscape.com)

These data identify a physiological model with which to identify signaling pathways that mediate cross-talk between p63alphaDeltaN and activating p53 kinase pathways after DNA damage in basal cell populations. (dundee.ac.uk)

however these treatments have limited efficacy and induce significant toxic effects on healthy cells. (unisalento.it)
However, they did not induce the de novo synthesis of leukotriene C 4 or prostaglandin D 2 from human basophils or any type of mast cell examined. (johnshopkins.edu)

The cytotoxicity on different cell lines was assayed using the 3-(4, 5-dimethylthiazol-2yl)-2, 5-diphenyltetrazolium bromide (MTT) assay which measures survival based on the detection of mitochondrial activity and by the crystal violet assay, which measures survival based on the ability of cells to remain adherent to microplates. (scielo.br)

The researchers found that BAM treatment to skin cells from fetuses and newborns didn't have the same effect as it did on the stem cells. (stanforddaily.com)
Researchers have succeeded in growing the most lifelike human skin in the lab to date. (alleninstitute.org)
They turned to cell lines from the Allen Cell Collection , a suite of human stem cells gene-edited by researchers at the Allen Institute for Cell Science, a division of the Allen Institute, to tag certain parts of the cell with fluorescent labels. (alleninstitute.org)
The researchers say that cells from women could be used to produce sperm, but that sperm would only be able to produce female babies because they lack a Y chromosome. (naturalnews.com)
Researchers then directed the stem cells to become RGCs, at which point the cells began adopting features specific to RGCs - features that were different in the cells of individuals with glaucoma than in the cells that came from healthy individuals. (siasat.com)
Through moving findings between monkey cells and human cells, the researchers were able to develop a successful method. (ohsu.edu)
Prof. Jacob Hanna (center) and his team of researchers working on the development of the stem-cell embryo models. (israel21c.org)

Identifi cation of human polyomavirus 9 among humans. (cdc.gov)
Merkel cell polyomavirus (MCPyV), and human the identity of the sources was removed from the samples, polyomavirus 9 (HPyV9), which was identifi ed in 2010 in and they were analyzed anonymously. (cdc.gov)
However, accurate hazard identifi- tify intrinsic hazard (skin sensitization) asso- cation of skin sensitizers represents only the first ciated with the substance can also, to varying step in a much more substantial process of safety degrees, provide information on the relative and risk assessment. (cdc.gov)

The Fuchs lab drove this point home in a 2017 study published in Nature by demonstrating that mouse skin that had recovered from irritation healed 2.5 times faster than normal skin when exposed to irritation at a later date. (scitechdaily.com)

In cytotoxicity assays, the A. niger- fermented extracts were nontoxic to CCD-966SK cells, even at 500 μ g/mL. (hindawi.com)
In the present investigations, we have sought to consolidate further our understanding of the association between EC3 values and human skin-sensitization potency by undertaking a thorough and extensive analysis of existing human predictive assays, particularly where dose- response information is available, from historical human repeated insult patch tests (HRIPTs). (cdc.gov)

While previous studies of cellular aggregates derived from human stem cells could not be considered accurate human embryo models because they lacked many of the defining characteristics of a post-implementation embryo, the Weizmann synthetic embryo models had all the structures characteristic of this stage, such as the placenta and yolk sac. (israel21c.org)
A stem cell-derived human embryo model equivalent to a day-14 embryo has all the compartments that define this stage: the yolk sac (yellow) and the part that will become the embryo itself, topped by the amnion (blue) - all enveloped by cells that will become the placenta (pink). (israel21c.org)
The 14-day human embryo model under the microscope reveals the hormone used in pregnancy tests (green) and the outer layer slated to become the placenta (pink), which contains characteristic cavities, called lacunae. (israel21c.org)

Some of these were derived from reprogrammed adult skin cells and others were the progeny of lab-cultured human stem-cell lines. (israel21c.org)

It involves transplanting the nucleus of one cell, containing an individual's DNA, into an egg cell that has had its genetic material removed. (ohsu.edu)
Metaphase is a stage in the cell's natural division process (meiosis) when genetic material aligns in the middle of the cell before the cell divides. (ohsu.edu)
This paper will discuss the evidence 7-Methyluric Acid both cellular and genetic for NK cell involvement in psoriasis. (bio2009.org)
There is marked angiogenesis and infiltration of immune cells into the skin [1 2 The cause of psoriasis is still unknown although it is clear that there is a strong genetic component to the disease. (bio2009.org)
1991. Genetic activity of the human carcinogen sulphur mustard towards salmonella and the mouse bone marrow. (cdc.gov)

The breakthrough follows similar success with monkey skin cells in 2007. (bigthink.com)

The amino acid sequence of human skin chymase was established by protein methods and by analysis of PCR amplification products obtained with cDNA-derived from urticaria pigmentosa (UP) lesions. (duke.edu)
skin lesions. (bio2009.org)
Human papillomavirus genotyping using next generation sequencing (NGS) in cervical lesions: Genotypes by histologic grade and their relative proportion in multiple infections. (who.int)

In our results, significant cytotoxicity was induced by ionic liquids with [TFSI] in both cell lines. (elsevierpure.com)

It is highly recommended to perform cell manufacturing for therapeutic use in a well-designed GMP-compliant cleanroom facility. (biomedcentral.com)
and as a bioactive component of an artificial skin, may have therapeutic value. (indexindex.com)
One important distinction is that while the method might be considered a technique for cloning stem cells, commonly called therapeutic cloning, the same method would not likely be successful in producing human clones otherwise known as reproductive cloning. (ohsu.edu)

Cell Rep Med;1(8): 100132, 2020 11 17. (bvsalud.org)

Allen Institute for Cell Science cell lines formed the basis for the human skin organoids. (alleninstitute.org)
The skin grows in tiny, oddly shaped clusters or cysts known as organoids. (alleninstitute.org)
The ultimate, long-term goal is to use these organoids for skin reconstructive surgery," said Koehler, who is an assistant professor at Boston Children's Hospital and Harvard Medical School . (alleninstitute.org)
We're hoping we can use skin organoids kind of like seeds to generate new skin in a wound. (alleninstitute.org)
Unlike some other types of organoids, which grow in spheres, the skin organoids sprout fleshy tentacles, Koehler said, like tiny octopi. (alleninstitute.org)
The team used one of the first stem cell lines released from the collection as the basis for their human skin organoids. (alleninstitute.org)
Applications of Human Pluripotent Stem Cell-Derived Skin Organoids in Dermatology. (invivogen.com)

HiFive cells maintained in Grace medium between MCPyV and chimpanzee polyomaviruses, and (Invitrogen) were infected with the different recombinant between HPyV9 and simian lymphotropic polyomavirus baculoviruses for the production of the different VLPs. (cdc.gov)

During the past three decades, research has shown the importance of these ingredients among the various antioxidant components of the skin. (mattek.com)
However, the topical application of antioxidants as delivered from cosmetic, skin-care, and over-the-counter (OTC) drug products have been shown to help boost the skin's antioxidant capacity. (mattek.com)
The physiological characteristics of the fermented extracts, namely, tyrosinase inhibitory activity, antioxidant activity, antibacterial activity, and anti-skin-aging activity, were evaluated and compared with those of unfermented extracts. (hindawi.com)
The highly concentrated treatment targets everything from skin tags to warts and acne with anti-inflammatory and antioxidant ingredients. (outlookindia.com)
Vitamins are part of the antioxidant system of human skin, and are detectable in different layers, so the topical application can be an alternative to maintain the functionality of the system. (intechopen.com)

DENV as a mosquito-borne virus infects human hosts via the skin, however the initial target cells in the skin remain unclear. (nih.gov)
This change in T cell homing potential is affected by the microenvironment where initial antigen recognition occurred, as memory T cells preferentially return to regions of the body similar to those where the initial antigen was encountered [ 1 ]. (biomedcentral.com)
By observing the numbers of cells attached and the area occupied by the cells, we concluded that asiaticoside also enhanced the initial skin cell adhesion. (indexindex.com)
Nevertheless, the initial need for viral-mediated gene delivery renders this strategy unsafe in humans. (nih.gov)
A follow-up investigation of rat breeders who supplied the initial patient's rats revealed six additional human cases of Seoul virus infections occurring at two Illinois rat-breeding facilities. (cdc.gov)
Initial efforts to centration of the chemical required to produce a demonstrate a relationship between predictive stimulation of proliferation of 3 compared with testing in the LLNA and human potency were the vehicle-treated control, the EC3 value, was undertaken in a highly qualitative manner (11). (cdc.gov)

Dual-immunofluorescence also revealed that Ser392 and Ser15 phosphorylation of p53 also occur in the same basal cells, although often within distinct regions of the nucleus. (dundee.ac.uk)

Anatomy14

Organisms4

Diseases14

Chemicals and Drugs17

Analytical, Diagnostic and Therapeutic Techniques and Equipment14

Phenomena and Processes23

Disciplines and Occupations1

Information Science1

Health Care3

Keratinocytes14

Fibroblasts9

Tissue20

Pluripotent stem5

Embryonic8

Cutaneous8

Vitro6

Mast Cells10

Epidermis7

Fragility of human2

Melanoma4

Cancer13

Exposure7

Diseases11

Viability2

Tissues5

Dendritic cells2

Melanocytes1

Neurons6

Toxicity3

Endothelial cells2

Radiation3

Embryos2

Somatic5

Proteins1

Vivo1

Grafts2

Keratinocyte1

Differentiate3

Therapies3

Biology2

Immunity6

Functional2

Squamous cell1

Immunodeficiency virus2

Carcinogenesis1

Basal cell1

Induce2

Adherent1

Researchers7

Identifi3

Irritation1

Assays2

Placenta3

Adult skin cells1

Genetic5

Monkey skin cells1

Lesions3

Cytotoxicity1

Therapeutic3

20201

Organoids7

Recombinant1

Antioxidant5

Initial6

Nucleus1