Human pluripotent stem cell cardiomyocytes cells into cardiomyocytes. Medical search. Frequent questions

Her work centers on using human embryonic and induced pluripotent stem cells to develop a heart patch that could be used to study the safety and efficacy of new drugs as well as its compatibility with various cell lines. (wikipedia.org)
Radisic was the first author of a highly cited paper in PNAS where she successfully generated beating heart tissues from embryonic stem cells via electrical stimulation. (wikipedia.org)
Human embryonic kidney cells 293 (HEK-293) and hPREP knockout cell line created from them by using CRISPR/Cas9-silencing were used in the experiments. (helsinki.fi)
The surgical procedure for delivery of embryonic stem cell-derived endothelial cells to the ischemic hindlimb is demonstrated, with non-invasive tracking by bioluminescence imaging. (jove.com)
To overcome this shortage, regenerative medicine using human pluripotent stem cells (hPSCs), such as human embryonic stem cells and human-induced pluripotent stem cells (hiPSCs), has been considered an alternative to HT. (bvsalud.org)
As an alternative to HT, cardiac regenerative therapy using human pluripotent stem cells (hPSCs), such as human embryonic stem cells and induced pluripotent stem cells, is expected to be realized. (bvsalud.org)
Based on their origin, there are two main types of stem cells - embryonic stem cells (ES cells) and postnatal or adult stem cells (AS cells). (bvsalud.org)
Embryonic stem cells are stem cells derived from the inner cell mass of an early, preimplantation stage embryo known as a blastocyst. (bvsalud.org)

In this study, we demonstrated an approach using human induced pluripotent stem cell‐derived atrial and ventricular cardiomyocytes (hiPSC‐aCMs and hiPSC‐vCMs, respectively) coupled with a sophisticated optical mapping system for drug screening of atrial‐selective compounds in vitro. (sfu.ca)
Human-induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) have shed great light on cardiac regenerative medicine and specifically myocardial repair in heart failure patients. (biomedcentral.com)
Uninterrupted monitoring of drug effects in human-induced pluripotent stem cell-derived cardiomyocytes with bioluminescence Ca2+ microscopy. (ox.ac.uk)
She previously worked as a postdoctoral scientist for two years in GlaxoSmithKline's Safety Pharmacology group, where her research focused on developing and validating an electrophysiology assay using human induced pluripotent stem cell-derived cardiomyocytes for cardiac safety screening. (the-scientist.com)
Induced pluripotent stem cell-derived cardiomyocytes beating in substrate. (medgadget.com)
Replating Protocol for Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes. (rndsystems.com)
Current methods for the maturation of induced pluripotent stem cell-derived cardiomyocytes. (wjgnet.com)
We analyzed standard reference RNA samples and RNA samples obtained from human induced pluripotent stem cell derived cardiomyocytes (hiPSC-CMs). (biomedcentral.com)
Cardiac Toxicity From Ethanol Exposure in Human-Induced Pluripotent Stem Cell-Derived Cardiomyocytes. (stembook.org)
Human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CM) can be used to identify and evaluate the effects of potentially cardiotoxic compounds. (moleculardevices.com)
We demonstrate that heart-derived collagen induces better contraction and relaxation of human induced pluripotent stem cell-derived EHT (hiPSC-EHT) and that hiPSC-EHT with heart-derived collagen exhibit more mature profiles than those with collagens from other organs. (bvsalud.org)
A study by Hashem et al using induced pluripotent stem cell-derived cardiomyocytes indicated that LAMP2 protein deficiency in Danon disease results in autophagic flux impairment, which in turn causes excessive oxidative stress and, subsequently, cardiomyocyte apoptosis. (medscape.com)

The ability of the human induced pluripotent stem cells (hiPSCs) to differentiate to any cell type including cardiomyocytes, while carrying patients' complex genetic backgrounds, has made them a promising and powerful tool for drug screening, assessing the cardiotoxicity of chemotherapeutic agents, and studying inherited cardiac diseases in vitro by recapitulating their cellular phenotypes. (sfu.ca)
Since the discovery of human induced pluripotent stem cells (hiPSCs), numerous strategies have been established to efficiently derive cardiomyocytes from hiPSCs (hiPSC-CMs). (healthdatagateway.org)
The FLIPR Penta system allows you to measure and analyze peaks of human-derived induced pluripotent stem cells (hiPSCs), differentiated into cardiomyocytes and neurons, up to 100 times per second and quickly cherry pick events such as Early Afterdepolarization-like events (EAD-like events), fibrillation, and irregular beating. (moleculardevices.com)

A Mount Sinai-led team has developed a reproducible and scalable method to advance maturation of human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs)-;cells that support heart muscle contraction, generated in the lab from human stem cell lines-;which researchers say will improve approaches for disease modeling, regenerative therapies, and drug testing. (azolifesciences.com)
The activation of signaling regulated by PPARd can further enhance heart muscle cell size and organization, and improve contractility, all hallmarks of heart maturation. (azolifesciences.com)
2022) PPARdelta activation induces metabolic and contractile maturation of human pluripotent stem cell-derived cardiomyocytes. (azolifesciences.com)
However, reshaping of the epigenome of these terminally differentiated cells during fetal development, postnatal maturation, and in disease remains unknown. (nature.com)
This study uncovers distinct layers of epigenetic regulation not only during prenatal development and postnatal maturation but also in diseased human cardiac myocytes. (nature.com)
Purification of CM nuclei by fluorescence-assisted sorting has led to the identification of cell-type-specific mCpG and histone modification signatures in CMs during mouse heart development and maturation 11 . (nature.com)
Based on this method 13 , 14 , we used a nuclear staining strategy to isolate CM nuclei from intact prenatal and postnatal human heart tissue and subjected these nuclei to comprehensive analysis of the epigenome during prenatal development, postnatal maturation, and in heart failure. (nature.com)
Here we describe the human CM epigenome during prenatal development and postnatal maturation of the heart from infant to adult age and in terminal failure. (nature.com)
The system utilized electrical stimulation to increase the maturation of hPSC derived cardiomyocytes. (wikipedia.org)
These lab-fabricated, fluorescently stained cardiomyocytes-heart muscle cells-exhibit the maturation and functionality of heart cells grown within a heart. (medgadget.com)
Co-differentiation and Co-maturation of Human Cardio-pulmonary Progenitors and Micro-Tissues from Human Induced Pluripotent Stem Cells. (rndsystems.com)
Monitoring cardiac differentiation and maturation from human pluripotent stem cells (hPSCs) and detecting residual undifferentiated hPSCs are indispensable for the development of cardiac regenerative therapy. (bvsalud.org)

Cardiac in vitro models have become increasingly obtainable and affordable with the optimization of human pluripotent stem cell-derived cardiomyocyte (hPSC-CM) differentiation. (mssm.edu)
A well-established method of cardiomyocyte differentiation from PSCs provides an ideal source of cardiomyocytes and makes regenerative therapy for myocardial muscle damage a promising strategy. (biomedcentral.com)
Induces differentiation of cardiomyocytes from human ESCs and iPSCs. (rndsystems.com)
Robust cardiomyocyte differentiation from human pluripotent stem cells via temporal modulation of canonical Wnt signaling. (rndsystems.com)
Differentiation of human epidermal neural crest stem cells (hEPI-NCSC) into virtually homogenous populations of DArgic neurons. (rndsystems.com)
Recapitulating human cardio-pulmonary co-development using simultaneous multilineage differentiation of pluripotent stem cells. (rndsystems.com)
Differentiation of hPSCs into cardiomyocytes (CMs) is facilitated by mimicking normal heart development. (bvsalud.org)

Although iPSC-derived cardiomyocytes have not yet replaced well-established FDA-approved toxicological methods, the FDA has fast-tracked efforts. (the-scientist.com)
Her current research focuses on utilizing cellular impedance measures of human iPSC-derived cardiomyocytes to deconvolute kinase inhibitor cardiotoxicity. (the-scientist.com)
Specialized cardiomyocytes like human induced pluripotent stem cell (iPSC)-derived cardiomyocytes are especially attractive cell models because they express GPCRs and ion channels that provide gene expression profiles as well as phenotypic characteristics while demonstrating spontaneous mechanical and electrical activity similar to native cardiac cells. (moleculardevices.com)
iPSC-derived cardiomyocytes form a synchronously beating monolayer that can be used to reliably reproduce drug-associated, cardio-physiologic phenotypes using a fast, kinetic fluorescence assay that monitors changes in intracellular calcium oscillations. (moleculardevices.com)
The assays use iPSC-derived cardiomyocytes loaded with a calcium-sensitive dye and allow you to monitor the compound impact on oscillating calcium within the cells as they beat. (moleculardevices.com)

Radisic and her team developed a microfabricated system for generating 3D, aligned beating cardiac tissue (Bio-wire) from human pluripotent stem cells (hPSC) derived cardiomyocytes. (wikipedia.org)
Cell therapy using hPSC-derived cardiomyocytes is expected to serve as an integral component of realistic medicine in the near future and is being potentially viewed as a treatment that would revolutionize the management of patients with severe heart failure. (bvsalud.org)

The protocol maintains CM functionality, allows cryopreservation, and the cells can be used in downstream assays such as disease modeling, drug and toxicity screening, and cell therapy. (healthdatagateway.org)
The Scientist brings together a panel of experts to educate you about the successes and caveats of using iPSC-derived cells in toxicological assays. (the-scientist.com)
Therefore, there is a growing need for highly predictive in vitro cardiotoxicity assays that use biologically relevant 3D cell-based models and are suitable for high-throughput screening. (moleculardevices.com)
Optogenetic assays provide a flexible, scalable, and information rich approach to probe compound effects for ion channel drug targets in both heterologous expression systems and associated disease relevant cell types. (frontiersin.org)
To evaluate the Swarm screening system, we optimized a series of heterologous optogenetic spiking HEK293 cell assays for several voltage-gated sodium channel subtypes including Nav1.2, Nav1.5, and Nav1.7. (frontiersin.org)
In summary, the Swarm is a novel high-throughput all-optical system capable of collecting information-dense data from optogenetic assays in both heterologous and iPS cell-derived models, which can be leveraged to drive diverse therapeutic discovery programs for nervous system disorders and other disease areas involving excitable cells. (frontiersin.org)

However it remains unknown which endocytic pathways are active in brain endothelial cells. (helsinki.fi)
In present study we characterized human brain endothelial cells, hCMEC/D3, which are widely utilized as BBB in vitro model. (helsinki.fi)
As brain endothelial cells are polarized in vivo, the aim of the study was to demonstrate the cell polarization of hCMEC/D3 cells and to study the activity and functionality of different endocytic pathways as a function of cell polarization. (helsinki.fi)
Nowadays, more and more studies have revealed that cells in the cardiovascular system (such as cardiomyocytes, vascular smooth muscle cells, endothelial cells, et al. (frontiersin.org)
The endothelial glycocalyx, a glycosaminoglycan layer located on the apical surface of vascular endothelial cells, has been shown to be important for several endothelial functions. (bioinf.org)

I showed that ibrutinib has a dramatic impact on the cardiac electrophysiology of hPSC-derived atrial cardiomyocytes, without affecting hPSC-derived ventricular cardiomyocytes. (sfu.ca)

Berend van Meer has won the Hugo van Poelgeest prize 2020 for his research on human pluripotent stem cell derived cardiomyocytes for disease modelling and drug discovery. (tradecast.eu)

Researchers at Michigan Tech and Harvard Medical School have developed a new way to create cardiomyocytes from induced pluripotent stem cells (iPSCs), for improved cardiac cell therapy. (medgadget.com)
iPSCs show significant potential for cardiac regenerative medicine, as researchers can use biochemical cues to "program" them into a cardiomyocyte-like cell, which shows regenerative efficacy in damaged cardiac muscle. (medgadget.com)
iPSCs have the great advantage that they can be generated from adults suffering from a known disease and then converted ("differentiated") into any cell type in the body. (ca.gov)
Our lab has been tasked with converting control and patient blood or skin cells, collected by scientists throughout the state of California, into high quality iPSCs. (ca.gov)

Our main focus is neural lineage (e.g. neurons and astrocytes), but other cell types can also be recorded (e.g. cardiomyocytes, β cells). (lu.se)

Ranolazine rescues the heart failure phenotype of PLN-deficient human pluripotent stem cell-derived cardiomyocytes. (stembook.org)
Here we study the cellular phenotype of hPSC-CMs by comparing their single-cell gene expression and functional profiles in three engineered cardiac tissue configurations: human ventricular (hv) cardiac anisotropic sheet, cardiac tissue strip, and cardiac organoid chamber (hvCOC), with spontaneously aggregated 3D cardiac spheroids (CS) as control. (mssm.edu)
By combining GmNL(Ca2+) with this adaptation, we could image spontaneous Ca2+ transients in cultured cardiomyocytes and phenotype their response to well-known drugs without accessing the sample directly. (ox.ac.uk)

Current drug development efforts for the treatment of atrial fibrillation are hampered by the fact that many preclinical models have been unsuccessful in reproducing human cardiac physiology and its response to medications. (sfu.ca)
In Chapter 3, I used human pluripotent stem cell (hPSC)-derived ventricular and atrial cells to study the toxicity of ibrutinib, a novel Bruton's tyrosine kinase (BTK) inhibitor, which has demonstrated benefit in B cell cancers, but is associated with atrial fibrillation. (sfu.ca)

OBJECTIVE: Cardiomyocytes derived from human-induced pluripotent stem cells are a powerful platform for high-throughput drug screening in vitro. (ox.ac.uk)
Neural crest stem cells from human epidermis of aged donors maintain their multipotency in vitro and in vivo. (rndsystems.com)
These features are important to better understand in vitro cardiomyocyte function and the impact of toxic compounds that induce cardiac abnormalities such as hERG blocking potassium channels. (moleculardevices.com)
Located on B10 , inside the cell culture laboratory, this set-up is ideal for , but not restricted to, investigation of in vitro generated and expanded cell lines and cultures. (lu.se)
Patch clamp recording in vitro (cell cultures) and, to some extent, ex vivo (tissue preparations) to study intrinsic electrical properties of single cells, such as resting membrane potential, membrane resistance, action potential parameters, presence and function of various channels and receptors as well as synaptic connectivity, and to identify, characterize and quantify synaptic transmission. (lu.se)

Cells from the blood samples are reprogrammed into clinical-grade human induced pluripotent stem cells, which can be distributed to recipients for regenerative medicine. (wjgnet.com)

Functional CMs derived from human pluripotent stem cells (hPSCs) would provide an unprecedented cell source for disease modeling, drug discovery, and cell transplantation therapy for CVD patients [ 3 ]. (biomedcentral.com)
Cell labeling, tracking, and molecular biology techniques indicated that the grafted cardiomyocytes survived in the rat heart 1 month after iPSC-CM transplantation. (biomedcentral.com)
Human iPSC-CM grafts survived in infarcted rat hearts and restored myocardial function 4 weeks after transplantation. (biomedcentral.com)
Heart regenerative therapy, such as transplantation of myocytes [ 4 ] and cardiomyocytes to replace lost cells, has become a new strategy for the treatment of heart failure caused by MI [ 5 ]. (biomedcentral.com)
We also discussed the advantage and challenges of mitochondrial transfer strategies, including cell-based mitochondrial transplantation, extracellular vesicle-based mitochondrial transplantation, and naked mitochondrial transplantation, for the treatment of cardiovascular disorders. (frontiersin.org)
Several issues, including the methods of large-scale culture and production of hPSCs and cardiomyocytes, the prevention of tumorigenesis secondary to contamination of undifferentiated stem cells and non-cardiomyocytes, and the establishment of an effective transplantation strategy in large-animal models, need to be addressed to fulfill this unmet need. (bvsalud.org)

Production of a novel cellularised & bioresorbable osteochrondral plug activating mesenchymal stem cells for joint repair. (lboro.ac.uk)
The expansion and recovery of human mesenchymal stem cells in disposable bioreactor platforms. (lboro.ac.uk)
These dental stem cells are considered mesenchymal stem cells (MSCs) and possess different levels of capacities to become specific tissue forming cells. (bvsalud.org)

Transcriptome analysis has enhanced our understanding of the molecular constituents of cells and tissues. (biomedcentral.com)
All tissues originate from stem cells. (bvsalud.org)
A stem cell is commonly defined as a cell that has the ability to continuously divide and produce progeny cells that differentiate (develop) into various other types of cells or tissues. (bvsalud.org)
Postnatal stem cells have been sourced from umbilical cord blood, umbilical cord, bone marrow, peripheral blood, body fat, and almost all body tissues, including the pulp tissue of teeth 8 . (bvsalud.org)

Non-thermal disruption of β-adrenergic receptor-activated Ca2+ signalling and apoptosis in human ES-derived cardiomyocytes by microwave electric fields at 2.4 GHz. (swansea.ac.uk)

Ion-channel block can lead to acute and delayed arrhythmias, while biochemical toxicities can result in cell death, abnormal cardiac function, and even heart failure and death. (the-scientist.com)
Specific decreasing of Na+ channel expression on the lateral membrane of cardiomyocytes causes fatal arrhythmias in Brugada syndrome. (ritsumei.ac.jp)

This work will create exciting opportunities to further assess human heart biology through multi-disciplinary approaches incorporating developmental biology, transcriptomics, contractile measurements and drug testing. (azolifesciences.com)
NIEHS research uses state-of-the-art science and technology to investigate the interplay between environmental exposures, human biology, genetics, and common diseases to help prevent disease and improve human health. (nih.gov)

Mitochondria not only serve as power plants in cells but also act as crucial regulators in many biological processes, including reactive oxygen species (ROS) signaling, redox balance, calcium homeostasis, protein quality control, and programmed cell death ( 3 , 4 ). (frontiersin.org)
The system can also monitor changes in intracellular Ca2+ flux (calcium oscillations) associated with cardiomyocyte contractions using the EarlyTox Cardiotoxicity Kit . (moleculardevices.com)
ScreenWorks® Peak Pro Software offers the ability to analyze multi-peak calcium oscillation responses of cells-in particular, cardiomyocyte beat rate as well as temporal characteristics of peaks including rise, decrease, and amplitude. (moleculardevices.com)
Spontaneous calcium oscillation acquired with FLIPR Calcium Assay Kit and the FLIPR system from iPSC cardiomyocytes or 3D cardiac spheroids can be paired with cytotoxicity and mitochondrial integrity data from the ImageXpress confocal system for comprehensive phenotypic analysis and bioactivity profiling of both pharmaceutical and environmental compounds. (moleculardevices.com)
To address this challenge, we developed the Swarm TM , a custom designed optical instrument for highly parallelized, multicolor measurements in excitable cells, simultaneously recording changes in voltage and calcium activities at high temporal resolution under optical stimulation. (frontiersin.org)
As a demonstration of the versatility of the Swarm, we also developed an assay measuring cardiac action potential and calcium waveform properties simultaneously under paced conditions using human induced pluripotent stem (iPS) cell-derived cardiomyocytes as an additional counter screen for cardiac toxicity. (frontiersin.org)
The B10 set-up consists of a HEKA EPC10 double-patch clamp amplifier, an Olympus BX51WI microscope with multi-channel fluorescence capability and differential interference contrast for live imaging and optical manipulation of cells (e.g. optogenetics and calcium imaging), and a Hamamatsu C11440-10C camera. (lu.se)

In addition, our study provides a functional map of the non-coding genome of human CMs throughout life. (nature.com)
Animals injected with cell-free solution (control group) demonstrated significant left ventricular (LV) functional deterioration, whereas grafting of iPSC-CMs attenuated this remodeling process. (biomedcentral.com)
Myocardial infarction (MI), the most common heart disease, is characterized by a significant reduction in the number of functional cardiomyocytes and leads to the development of progressive heart failure. (biomedcentral.com)
However, creating fully functional cardiac cells can be a challenge. (medgadget.com)
Unfortunately stem cell therapeutics don't have high success rates partly because the cells are not mature and fully functional. (medgadget.com)
This results in more robust, mature and functional cardiomyocyte-like cells. (medgadget.com)
In pathological conditions, damaged cells transfer dysfunctional mitochondria toward recipient cells to ask for help and take up exogenous functional mitochondria to alleviate injury. (frontiersin.org)
They demonstrated that transferring functional mitochondria of bone marrow-derived stem cells to defective parenchymal cells increases the aerobic respiration capacity of recipient mitochondria ( 7 ). (frontiersin.org)

The team developed a culture mold that mimics the physiological conditions under which cardiomyocytes grow. (medgadget.com)
Studies have shown that cell-to-cell mitochondrial transfer plays an essential role in regulating cardiovascular system development and maintaining normal tissue homeostasis under physiological conditions. (frontiersin.org)

Finding physiologically relevant, reproducible, and reliable cell models that can be used to detect these end points at preclinical and discovery stages has been a challenge, as most toxicity testing is done either by using immortalized cancer cell lines, primary explanted somatic cells, or live animals. (the-scientist.com)

Cardiomyocytes (CMs) derived from human pluripotent stem cells (hPSCs) provide a valuable cell source for regenerative therapy, disease modeling, and drug screening. (biomedcentral.com)
New insights into genetic disease using 3D engineered heart tissue made from human pluripotent stem cell cardiomyocytes. (lboro.ac.uk)
Companion diagnostic tool for Alzheimer's disease (cell) therapy developments. (lboro.ac.uk)
The researchers plan to test the cells in an animal model of cardiac disease, and also investigate the potential of enhancing electrical conductivity between the cells, to facilitate the cell-cell communication that occurs in the heart. (medgadget.com)
First year progress on grant ID1-06557, " Generation and Characterization of High-Quality, Footprint-Free Human Induced Pluripotent Stem Cell (iPSC) Lines From 3000 Donors to Investigate Multigenic Disease" has met all agreed-upon milestones. (ca.gov)
For example, iPSC lines from patients with heart disease can be converted into heart cells, iPSC lines from patients with Alzheimer's disease can be converted to brain cell, and iPSC lines from patients with pulmonary fibrosis can be converted into cells of the lung. (ca.gov)

She has also conducted research on developing strategies to engineer vascularized myocardium based on the tri-culture of key heart cell types using the engineered cardiac tissue as a model system for cardiac cell therapy or drug testing. (wikipedia.org)

The study of inherited human cardiovascular diseases has been hampered by limited access to cardiac tissue from patients harboring specific mutations, which are thought to be causal. (sfu.ca)
Owing to technical restrictions, these studies were performed in heart tissue and therefore the affected cell type(s) could not be identified. (nature.com)
Radisic is known for creating beating heart tissue in a dish using human-induced pluripotent stem cells. (wikipedia.org)
Her research has led the replication of diseased human heart tissue that can be used for drug screening to help create treatment therapies for patients with heart injury. (wikipedia.org)
Bio-wire is the first system combining electrical stimulation with geometry control of 3D tissue assembly to improve the electrical and ultra-structural properties of human cardiac tissue. (wikipedia.org)
The key elements of tissue engineering are stem cells, morphogen, and a scaffold of extracellular matrix. (bvsalud.org)
Optogenetic manipulation of cell function to study, e.g. synaptic profile of isolated populations of the cells in cell cultures and tissue preparations. (lu.se)
Optical imaging of live cells in cultures and tissue preparations to monitor intracellular Ca 2+ fluctuations and to identify population of interest. (lu.se)
Multielectrode Array (MEA) recordings of extracellular field potentials from cell cultures and tissue preparations. (lu.se)

To characterize hCMEC/D3 cells for the presence of specific endocytic pathways, proteins involved into each pathway were selected. (helsinki.fi)

Unlike the isogenic control cell line, irregular voltage- and Ca2+ transients and arrhythmic activities were observed in the presence higher rates of stimulation or β-adrenergic agonists in monolayer of R37C+/- TNNI1 hiPSC-CMs. (sfu.ca)
250-fold) of high-purity hiPSC-CMs relying on two aspects: removal of cell-cell contacts and small-molecule inhibition with CHIR99021. (healthdatagateway.org)

AN - check the tag INFANT HN - 2008 FX - Child Nutrition FX - Infant Nutrition Physiology FX - Milk FX - Milk, Human DH - Adolescent Nutrition DI - 052508 MN - SP6.021.067 MS - Nutrition of persons 10 through 19 years of age. (bvsalud.org)
check the tag ADOLESCENCE HN - 2008 BX - Nutrition in Adolescence FX - Adolescent Nutrition Physiology MH - Peritoneal Stomata UI - D054048 MN - A01.047.025.600.700 MN - A10.810 MS - Natural openings in the subdiaphragmatic lymphatic plexus in the PERITONEUM, delimited by adjacent mesothelial cells. (bvsalud.org)

Growing the cells in a three-dimensional substrate that mimics the natural heart environment produces better cells. (medgadget.com)
Using photolithography to shape a polydimethylsiloxane substrate and micropatterning to alter the substrate surface, the researchers developed 3D molds that mimic the natural forces and pressures that cardiomyocytes experience in the heart. (medgadget.com)

reported a protocol to selectively differentiate hPSCs into CMs with nodal-type characteristics, which relies on co-culture with visceral endoderm-like cells and subsequent culture in a serum-enriched medium [ 12 ]. (biomedcentral.com)

Investigating the potential of human umbilical cord derived-stem cells for chronic wound healing. (lboro.ac.uk)

The goal of CIRM Grant ID1-06557 is to generate high quality induced pluripotent stem cells (iPSC) from blood and skin samples from 3000 donors, many of whom suffer from untreatable medical conditions, and place them in a Repository accessible to scientists around the world. (ca.gov)

Rather than focusing on biochemical cues, these researchers looked at modifying the 3D environment of the cells, with a view to creating an environment more like that found in the heart. (medgadget.com)
Programming induced pluripotent stem cells into heart muscle cells requires biochemical and biomechanical cues. (medgadget.com)

These UCNPs allow programmable activation of two synergistic light-gated ion channels VChR1 and Jaws in the same cell to manipulate membrane polarization, demonstrated here for cardiac pacing. (nature.com)

RESULTS: GmNL(Ca2+) gives a 140% signal change with Ca2+, and can image drug-induced changes of Ca2+ dynamics in cultured cells. (ox.ac.uk)
Extracellular Matrix From Hypertrophic Myocardium Provokes Impaired Twitch Dynamics in Healthy Cardiomyocytes. (rndsystems.com)
In particular, Cellular Dynamics International (CDI) has taken lease to approximately 5000 square feet of lab space at the Buck Institute for Research on Aging in Novato, CA. The majority of this space is located within the new CIRM-funded Stem Cell Research Building at the Buck Institute and was extensively reconfigured to meet the specific needs of this grant. (ca.gov)

A study reporting this new protocol was published in the April 7 print edition of the journal Cell Stem Cell . (azolifesciences.com)

The work of Berend aimed to understand how well these stem cell based cardiac models can predict the effect of (well-known) drug therapies in patients. (tradecast.eu)
Literature review: This review summarizes current knowledge, barriers, and challenges in the clinical use of adult stem cells, scaffolds, and growth factors for the development and evaluation of regenerative endodontic therapies. (bvsalud.org)
This explains why researchers are now focusing attention on developing stem cell therapies using postnatal stem cells donated by the patients themselves or their close relatives. (bvsalud.org)

While the signaling effects of the protein peroxisome proliferator activated receptor alpha (PPARa) are the most active in heart muscle cells, the researchers said PPARd signaling has a separate and important role in efficiently activating the gene regulatory networks, increasing the quantity and organization of the organelles involved in energy production, and augmenting the fatty acid oxidation process. (azolifesciences.com)
Given the complexity of the biological function of mitochondria, researchers have begun to consider rescuing the injured cells through mitochondrial transfer, that is, replacing damaged mitochondria with healthy mitochondria from donor cells. (frontiersin.org)

Our aim is to determine if the same pathway is followed in human cardiomyocytes derived from human inducible pluripotent stem cells. (syr.edu)
Autophagy is a pathway for cells to degrade intracellular components that are no longer needed or are detrimental for the cells. (helsinki.fi)

Here, we established a hPSC line integrated with a mCherry fluorescent protein driven by the alpha myosin heavy chain (aMHC) promoter, which could be used to purify CMs based on the aMHC promoter activity in these cells. (biomedcentral.com)
The research team also identified the role of the protein known as peroxisome proliferator activated receptor delta (PPARd) in inducing what is referred to as the metabolic switch in the lab-generated heart muscle cells. (azolifesciences.com)
We found that the human cardiomyocytes developed patterns of protein organization identical to the three-step series seen in the model organisms cited above. (syr.edu)
The survival of grafted cardiomyocytes was confirmed by observing the fluorescent cell tracer Vybrant™ CM-DiI or expression of the enhanced green fluorescent protein (eGFP) in transplanted cells, or survival was demonstrated by polymerase chain reaction (PCR)-based detection of human mitochondrial DNA. (biomedcentral.com)

The characterization of BBB cells would help light on the exact mechanism of nanoparticle delivery into the brain, which would enable the design of targeted nanoparticles to deliver drugs to the CNS. (helsinki.fi)

Sarcomere function activates a p53-dependent DNA damage response that promotes polyploidization and limits in vivo cell engraftment. (rndsystems.com)
completed, technical barriers to mass cell production will have been eliminated making transfusion with ex-vivo generated red cells a reality. (bioinf.org)

In summary, our results support a common three-step model for the formation of myofibrils ranging from avian to human cardiomyocytes. (syr.edu)
The objective of this study was to evaluate the ability of human iPSC-CMs to improve myocardial function in a rat postinfarction heart failure model. (biomedcentral.com)

2017). Assembly and maintenance of myofibrils in living muscle cells. (syr.edu)

Fluorescent stem cell reporters provide a convenient and efficient method to purify expected CM subtypes. (biomedcentral.com)

ES cells are pluripotent cells, which mean that they can give rise to all differentiated cell types derived from all three germ layers. (bvsalud.org)

Epigenetic mechanisms are highly cell-type-specific requiring cell separation techniques to determine epigenomic features in a specific cell type, especially when keeping in mind that the cellular composition of the human heart is highly dynamic. (nature.com)

The major areas of research that might have application in the development of regenerative endodontic techniques are (a) postnatal stem cells, (b) scaffold materials, (c) morphogen/growth factors, (d) implantation. (bvsalud.org)
The most valuable cells for regenerative endodontics are postnatal or adult stem cells. (bvsalud.org)

MicroRNA (miRNA) is secreted from cells into the extracellular space, and its role as a biomarker is attracting attention. (bvsalud.org)

Cardiovascular diseases (CVD) claim millions of lives per year and pose a severe threat to economics and human health. (biomedcentral.com)

They found that PPARd induces the metabolic switch from glycolysis to fatty acid oxidation in the lab setting, thus influencing whether heart muscle cells generate energy from glucose or fatty acids. (azolifesciences.com)
The research team also investigated the effects of lactate exposure, where heart muscle cells are able to survive on lactate in the absence of glucose. (azolifesciences.com)
Importantly, the outcomes of the experiments were compared to very similar measurements in rabbit heart muscle cells. (tradecast.eu)
The mechanical properties of substrates play an important role in the cell behavior because the mechanical cues that cells sense in the actual (heart) environment is unique," said Abadi. (medgadget.com)
Chris' primary research focus is on using experimental and clinical data to predict how heart cells, the engine of every heartbeat, respond to genetic mutations and to drugs. (swansea.ac.uk)

The Keller group described a transgene-independent method to generate sinoatrial node (SAN)-like pacemaker cells, which were identified as NKX2-5-negative CMs expressing SAN markers and displaying expected electrophysiological properties [ 13 ]. (biomedcentral.com)

The micropatterning alters the cell cytoskeleton and nuclear shape of cells cultured in the molds, causing changes in gene expression. (medgadget.com)
The Ion AmpliSeq™ Transcriptome Human Gene Expression Kit (AmpliSeq) was recently introduced by Life Technologies as a whole-transcriptome, targeted gene quantification kit to overcome these limitations of RNA-seq. (biomedcentral.com)
Senescence-induced immunophenotype, gene expression and electrophysiology changes in human amniocytes. (nih.gov)

The research contributes to understanding the relevance of stem cell based models and strengthens the confidence regulators and pharmaceutical companies have in such models as animal alternatives in the drug development pipeline. (tradecast.eu)
There are limited numbers of publications about ES cells in pulp regeneration, due to the restricted policies regarding ES cell research over the past few years. (bvsalud.org)

These cardiovascular adverse events may result from prevention of mature myofibril formation in the cardiomyocytes. (syr.edu)

The development of human induced pluripotent stem cell (iPSC)-derived cell lines as models for drug-toxicity testing offers a promising alternative that is more physiologically relevant, more predictive, and more time and cost efficient. (the-scientist.com)
Conn Hastings received a PhD from the Royal College of Surgeons in Ireland for his work in drug delivery, investigating the potential of injectable hydrogels to deliver cells, drugs and nanoparticles in the treatment of cancer and cardiovascular diseases. (medgadget.com)
Human pluripotent stem cell-derived cardiomyocytes (hPSC-CM) are being investigated as a new source of cardiac cells for drug safety assessment. (bioinf.org)

Anatomy41

Organisms12

Diseases4

Chemicals and Drugs32

Analytical, Diagnostic and Therapeutic Techniques and Equipment35

Phenomena and Processes32

Disciplines and Occupations5

Technology, Industry, Agriculture2

Embryonic8

Induced pluripotent12

HiPSCs3

Maturation12

Differentiation7

IPSC-derived cardiomyocytes5

HPSC derived cardiomyocytes2

Assays6

Endothelial cells5

Ventricular cardiomyocytes1

20201

IPSCs4

Neurons1

Phenotype3

Atrial fibrillation2

Vitro5

Regenerative medicine1

Transplantation6

Mesenchymal stem3

Tissues4

Apoptosis1

Arrhythmias2

Biology2

Calcium7

Functional8

Physiological conditions2

Somatic cells1

Disease6

Cardiac cell therapy1

Tissue9

Pathways1

HiPSC2

Physiology2

Substrate2

HPSCs1

Umbilical cord1

Donors1

Biochemical2

Membrane1

Dynamics3

Protocol1

Therapies3

Researchers2

Pathway2

Protein4

Characterization1

Vivo2

Model2

20171

Fluorescent1

Germ layers1

Cellular1

Postnatal2

Extracellular1

Severe1

Heart5

Generate1

Gene expression3

Research2

Prevention1

Drug3