• This material should be handled at the Biosafety Level 2 (BSL 2) as recommended for any potentially infectious human serum or blood specimen in the CDC/NIH manual "Biosafety in Microbiological and Biomedical Laboratories", 2009. (leebio.com)
  • A detailed patient history and clinical information is recorded at the time of draw, and multiple samples and plasma/serum matched sets from the same donor are often available. (innov-research.com)
  • Here, we explore this aspect of the protein binding capacity of bleomycin to the two most abundant plasma proteins , human serum albumin (HSA) and α1- acid glycoprotein (AGP), which are known to bind and to be carriers of many drug molecules using spectroscopic techniques , such as circular dichroism , UV-vis absorbance, and fluorescence . (bvsalud.org)
  • The active ingredient comprises plasma proteins such as albumin , immunoglobulins, other globulins, coagulation factors, complement proteins and protease inhibitors. (rxlist.com)
  • The content and distribution of plasma proteins in Octaplas are comparable to reference ranges for healthy blood donors, except for Protein S and alpha2-antiplasmin. (rxlist.com)
  • Using multiple sequence alignments of human erythroviruses and comparison with the sequence for PARV4 ( 1 ), we designed highly selective primers to the open reading frame 1 (ORF1) of PARV4, homologous to the nonstructural proteins of other parvoviruses. (cdc.gov)
  • Using a proteomic approach based on extensive protein fractionation, we confidently identified 1,442 proteins that were distributed across seven orders of magnitude of abundance in plasma. (nih.gov)
  • Analysis of proteins chosen on the basis of increased levels in plasma from tumor-bearing mice and corroborating protein or RNA expression in tissue documented concordance in the blood from 30 newly diagnosed patients with pancreatic cancer relative to 30 control specimens. (nih.gov)
  • A panel of five proteins selected on the basis of their increased level at an early stage of tumor development in the mouse was tested in a blinded study in 26 humans from the CARET (Carotene and Retinol Efficacy Trial) cohort. (nih.gov)
  • Nanoparticles can be used to purify proteins from plasma. (scirp.org)
  • Interaction of Blenoxane and Congeners Bleomycins A2 and B2 with Human Plasma Proteins Using Circular Dichroism Spectroscopy. (bvsalud.org)
  • The results showed that bleomycin binds to plasma proteins with an order-of- magnitude higher affinity for AGP than HSA. (bvsalud.org)
  • Blood donations in the United States have been screened for antibody to human immunodeficiency virus type 1 (HIV-1) since March 1985 and type 2 (HIV-2) since June 1992. (cdc.gov)
  • In the United States, the implementation of antibody testing in 1985 of all donated blood for human immunodeficiency virus type 1 (HIV-1) resulted in a substantial decrease in the transmission of HIV through blood transfusions (1,2). (cdc.gov)
  • The risk of mother-to-child transmission of human immunodeficiency virus (HIV) during pregnancy, delivery, and breastfeeding is as high as 25-30% in the absence of treatment. (medscape.com)
  • HA371 trade name] is indicated in combination with other antiretroviral agents for the treatment of human immunodeficiency virus (HIV) infection in adults, adolescents and children. (who.int)
  • Acquired immunodeficiency syndrome (AIDS) is essentially a sexually transmitted disease but like many other sexually transmitted diseases, the human immunodeficiency virus (HIV) which causes AIDS can also be transmitted through blood or blood products and from infected mother to baby. (who.int)
  • "Quantitation of N-ethyl-3,4-methylenedioxyamphetamine and its major metabolites in human plasma by high-performance liquid chromatography and fluorescence detection" J Chromatogr B Biomed Sci Appl . (erowid.org)
  • A HPLC method has been developed for the analogue of Ecstasy MDE and its major metabolites N-ethyl-4-hydroxy-3-methoxyamphetamine HME and 3,4-methylenedioxyamphetamine MDA in human plasma. (erowid.org)
  • Profile of plasma and urine metabolites after the intake of almond [prunus dulcis (mill. (nuthealth.org)
  • The aim of the present study was to determine the profiles of both phase II and microbial-derived phenolic metabolites in plasma and urine samples before and after the intake of almond skin polyphenols by healthy human subjects (n = 2). (nuthealth.org)
  • Comparison of plasma concentrations of BDE-47 with its known hydroxylated metabolites suggested OH-PBDE levels were likely determined by biotransformation and at least two subpopulations identified differing in their apparent rates of OH-PBDE formation. (cdc.gov)
  • The main metabolites of simvastatin in human plasma are beta-hydroxy acid and four additional active metabolites. (janusinfo.se)
  • A mouse to human search for plasma proteome changes associated with pancreatic tumor development. (nih.gov)
  • The complexity and heterogeneity of the human plasma proteome have presented significant challenges in the identification of protein changes associated with tumor development. (nih.gov)
  • The increased sensitivity of contemporary HIV-antibody EIAs, improved donor interviewing about behaviors associated with risk for HIV infection, and deferral of donors who test positive for HIV, hepatitis, human T-cell leukemia virus type 1 (HTLV-I), or syphilis have considerably improved the safety of the U.S. blood supply. (cdc.gov)
  • Human blood plasma is collected by centrifuging to remove just the plasma and returning the rest of the blood to the donor. (machineconcepts.co.uk)
  • Single Donor Human Prostate Cancer Plasma from Innovative Research is whole blood derived. (innov-research.com)
  • Plasma exchange involves replacing a part of a patient's blood plasma with donor plasma or a plasma substitute. (lifespan.io)
  • The manufacturing plasma pool is limited to 390 kg comprising 630-1,520 individual donors. (rxlist.com)
  • Antibody against human Hepatitis C, plasma collected from human donors. (calbiotech.com)
  • Homogeneous, single-chain human factor V was used to develop a double antibody competition radioimmunoassay to measure factor V concentrations in plasma and platelets. (nih.gov)
  • Routine testing for p24 antigen in settings other than blood and plasma centers as a method for diagnosing HIV infection is discouraged because the estimated average time from detection of p24 antigen to detection of HIV antibody is 6 days, and not all recently infected persons have detectable levels of p24 antigen. (cdc.gov)
  • IgG is the main antibody type found in plasma and extracellular fluid and is expressed on B cell membrane. (sigmaaldrich.com)
  • Normal factor V concentrations range from 4 to 14 micrograms/ml of plasma with an average value of 7.0 +/- 2.0 micrograms/ml (n = 64). (nih.gov)
  • The maximum plasma concentrations were 235-465 ng/ml MDE, 67-673 ng/ml HME and 7-33 ng/ml MDA, respectively. (erowid.org)
  • There was no correlation between plasma adiponectin concentrations and 24-hour RQ, (r = 0.09, p = 0.36) before or after adjustment for PFAT (r = 0.001, p = 0.99, respectively, partial correlation), and no correlation was found between plasma adiponectin concentrations and 24-hour EE (r = -0.12, p = 0.27). (monash.edu)
  • Discussion: Our cross-sectional data do not suggest physiological concentrations of fasting plasma adiponectin play a role in the regulation of whole-body fat oxidation or energy expenditure in resting conditions. (monash.edu)
  • In AMBAR, various groups received various concentrations (either 5% or 20%) of medicinal albumin in their plasma transfers. (lifespan.io)
  • Human myeloma IgG1, λ was used as non-specific control in quantitative ELISA and solid-phase ELISA. (sigmaaldrich.com)
  • Calbiotech specializes in immunoassay development and manufacturing, with a comprehensive range of ELISA and CLIA assays for human and animal research, including specific assays for hormone, autoimmune disorders, cancer, infectious diseases and much more. (calbiotech.com)
  • Title : Detection of Brevetoxin in Human Plasma by ELISA Personal Author(s) : Cunningham, Brady R.;Coleman, Rebecca M.;Schaefer, Adam M.;Hamelin, Elizabeth I.;Johnson, Rudolph C. (cdc.gov)
  • Samples of manufacturing plasma pools submitted to the National Institute for Biological Standards and Control for testing for hepatitis C virus RNA were stored at -70°C until analysis, in compliance with European regulatory requirements. (cdc.gov)
  • Arotinoid acid and the internal standard (IS), acitretin, were extracted from plasma samples using solid phase extraction with Oasis HLB cartridges. (unboundmedicine.com)
  • This sensitive, rapid and reliable analytical method has been used successfully in the quantitation of the substances in plasma samples obtained from 14 volunteers in two clinical studies after p.o. administration of 100 to 140 mg MDE*HCI. (erowid.org)
  • Home / Biologicals / Plasma / Samples / Human (blood-derived) / Plasma - Pediatric Samples - 5. (leebio.com)
  • Plasma - Pediatric Samples - 5. (leebio.com)
  • Glucuronide, O-methyl glucuronide, sulfate, and O-methyl sulfate derivatives of (epi) catechin, as well as the glucuronide conjugates of naringenin and glucuronide and sulfate conjugates of isorhamnetin, were detected in plasma and urine samples after consumption of almond skin polyphenols. (nuthealth.org)
  • We suggest that SWATH-MS analysis combined with N-glycoproteome enrichment in plasma samples is a promising integrative proteomic approach for biomarker discovery and verification. (nih.gov)
  • Application of this method to plasma samples revealed that at least 50 samples could be extracted in a routine day. (duke.edu)
  • We measured 32 different OH-PBDEs and MeO-PBDEs in plasma samples provided by 113 volunteers living in the greater Puget Sound region of Washington State, USA. (cdc.gov)
  • To investigate the pharmacokinetics of arotinoid acid, a sensitive and selective liquid chromatographic-tandem mass spectrometric method (LC-MS/MS) for the determination of arotinoid acid in human plasma was developed and validated. (unboundmedicine.com)
  • Therefore, an extraction protocol and LC/MS-MS method for the determination of daptomycin in human milk and plasma were developed, validated and applied to a case of a breastfeeding mother affected by a purulent acute soft skin infection treated with daptomycin. (unimi.it)
  • The determination of milk/plasma (M/P) ratio, which ranged from 0.002 to 0.006, allowed to assess that daptomycin, effective for the mother, was contemporarily safe for the breastfed newborn. (unimi.it)
  • Determination of daptomycin in human plasma and breast milk by UPLC/MS-MS / M. Dei Cas, E. Casagni, V. Gambaro, E. Cesari, G. Roda. (unimi.it)
  • MicroLC-MRMHR Workflow for Cyclic Peptide Quantitation in Human Plasma In biotherapeutic development, decreased drug doses and limited sample volumes require higher quantification sensitivity, while the complexity of biological matrices calls for the need of assay selectivity improvement. (technologynetworks.com)
  • Plasma deficient for Factor II assay. (cryopep.com)
  • Frozen, immuno-depleted plasmas are certified to have less than 1% for the element considered, both for the antigenic and functional assay in haemostasis. (cryopep.com)
  • Quantitative measurements of N-linked glycoproteins in human plasma by SWATH-MS. (nih.gov)
  • Scholars@Duke publication: A comprehensive method for extraction and quantitative analysis of sterols and secosteroids from human plasma. (duke.edu)
  • Importantly, most participants, in addition to albumin, also received immunoglobulin (antibodies) in their plasma transfers to correct for a possible immunological deficit, which is a known side effect of PE. (lifespan.io)
  • Lee Biosolutions is the leading collector and distributor of human biological fluids and tissues for protein isolation, diagnostic manufacturing and medical research. (leebio.com)
  • This network is an important forum for the confidential exchange of quality and technical information on human biological medicinal products and related methods and is a key link in the regulatory chain. (edqm.eu)
  • Refined genetically engineered mouse (GEM) models of human cancer have been shown to faithfully recapitulate the molecular, biological, and clinical features of human disease. (nih.gov)
  • Our findings indicate that GEM models of cancer, in combination with in-depth proteomic analysis, provide a useful strategy to identify candidate markers applicable to human cancer with potential utility for early detection. (nih.gov)
  • Despite this, limited data exist regarding the plasma proteomic changes that occur in response to regular exercise. (lu.se)
  • Here, we perform large-scale plasma proteomic profiling in 654 healthy human study participants before and after a supervised, 20-week endurance exercise training intervention. (lu.se)
  • Using dilution series of isotopically labeled heavy peptides representing biomarker candidates, the LOQ of SWATH-MS was determined to reach 0.0456 fmol at peptide level by targeted data analysis, which corresponds to a concentration of 5-10 ng protein/mL in plasma, while SRM reached a peptide LOQ of 0.0152 fmol. (nih.gov)
  • Following an oral dose of radioactive simvastatin in humans, 13% of the radioactivity is excreted in the urine and 60% in the faeces within 96 hours. (janusinfo.se)
  • Lee Biosolutions is the leading supplier of human plasma High Triglyceride Fraction for research and diagnostic manufacturing. (leebio.com)
  • Standard curves were constructed that allow for the detection of as little as 20 ng factor V/ml of plasma. (nih.gov)
  • Sterols and oxysterols are measured using triple quadrupole mass spectrometers, and lathosterol is measured using GC-MS. Detection for each compound measured by HPLC-MS was ∪ 1 ng/ml of plasma. (duke.edu)
  • With nitrendipine as the internal standard, sample pretreatment involved one-step extraction with diethyl ether of 0.5 ml plasma. (unboundmedicine.com)
  • We describe the development of a method for the extraction and analysis of 62 sterols, oxysterols, and secosteroids from human plasma using a combination of HPLC-MS and GC-MS. Deuterated standards are added to 200 μl of human plasma. (duke.edu)
  • The dosage of Octaplas for plasma exchange in patients with TTP is 1 to 1.5 plasma volumes (40 to60 milliliters per kg). (rxlist.com)
  • Nanometer-scale layers of demineralized and mineralized unstained human femoral lamellar bone were sequentially sectioned over volumes of 6-16,000 μm3. (mcmaster.ca)
  • In the present study, we demonstrate that following exposure to plasma from diabetic patients, the human promonocytic cell line U937 exhibits a significant elevation in core 2 GlcNAc-T activity and increased adherence to cultured retinal capillary endothelial cells. (diabetesjournals.org)
  • Total nucleic acid was extracted from plasma pools as described previously ( 4 ) before analysis for PARV4 DNA. (cdc.gov)
  • Prepared from plasma shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA-required tests. (athensresearch.com)
  • Chen R, Jiang X, Sun D, Han G, Wang F, Ye M, Wang L, Zou H: Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry. (hmdb.ca)
  • This project required some innovative solutions to the difficult requirement of cleaning a complex machine after exposure to human blood plasma, one of the best growing mediums for bacteria. (machineconcepts.co.uk)
  • The marked area from the intracellular plasma membrane-connected compartment (IPMC) is shown at higher magnification in ( b ). c View of the area marked in ( b ) to show the immature phenotype of the budding-arrested virus particles. (biomedcentral.com)
  • Without the plasma membrane, there would be no cell. (pressbooks.pub)
  • Although it is very thin and flexible, the plasma membrane protects and supports the cell by controlling everything that enters and leaves it. (pressbooks.pub)
  • To understand how the plasma membrane controls what passes into or out of the cell, you need to know its basic structure. (pressbooks.pub)
  • The plasma membrane is composed mainly of phospholipids, which consist of fatty acids and alcohol. (pressbooks.pub)
  • molecules, on the other hand, cannot pass through the plasma membrane - at least not without help - because they are water-loving like the exterior of the membrane. (pressbooks.pub)
  • Molecules of the steroid lipid cholesterol help the plasma membrane keep its shape. (pressbooks.pub)
  • Figure 4.4.4 The plasma membrane contains many molecules embedded in the lipid bilayer. (pressbooks.pub)
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. (sigmaaldrich.com)
  • Not for human consumption. (rpeptide.com)
  • Quickly frozen at -80° C, the plasma maintains perfectly intact the matrix. (cryopep.com)
  • Pharmacokinetic parameters have been investigated using the plasma concentration curves. (erowid.org)
  • abstract = "Objective: To test the hypothesis that low adiponectin is associated with low fat oxidation in humans. (monash.edu)
  • These effects of diabetic plasma on enzyme activity and cell adhesion, mediated by PKCβ2-dependent phosphorylation of the core 2 GlcNAc-T protein, were found to be triggered by increased plasma levels of tumor necrosis factor (TNF)-α. (diabetesjournals.org)
  • Here, we sought to exploit the merits of a well-characterized GEM model of pancreatic cancer to determine whether proteomics technologies allow identification of protein changes associated with tumor development and whether such changes are relevant to human pancreatic cancer. (nih.gov)
  • Plasma was sampled from mice at early and advanced stages of tumor development and from matched controls. (nih.gov)
  • To assess the performance and suitability of SWATH-MS-based protein quantification for clinical use, we compared SWATH-MS and SRM-MS-based quantification of N-linked glycoproteins in human plasma, a commonly used sample for biomarker discovery. (nih.gov)
  • AMBAR (Alzheimer's Management by Albumin Replacement) was a clinical trial conducted by the Spanish pharmaceutical company Grifols to study the effects of plasma exchange (PE) on AD. (lifespan.io)
  • This plasma is stored at -40 C and is processed in large quantities to extract the various factors, albumin etc. (machineconcepts.co.uk)
  • Statistically significant slowing in AD progression was observed in the range of 42%-70%, depending on the test, the amount of albumin in the fresh plasma, and the severity of the disease. (lifespan.io)
  • Plasma frozen, immunodepleted, poor in platelets and certified to have less than 1% in FII. (cryopep.com)
  • Levels of enzyme activity in plasma-treated U937 cells were closely dependent on the severity of diabetic retinopathy, with the highest values observed upon treatment with plasma of patients affected by proliferative retinopathy. (diabetesjournals.org)
  • To evaluate the proliferative capacity of mesenchymal cells derived from human periodontal ligament on polished and plasma-treated titanium surfaces. (bvsalud.org)
  • Overall, SWATH-MS measurements showed a slightly lower sensitivity and a comparable reproducibility to state-of-the-art SRM measurements for targeted quantification of the N-glycosites in human blood. (nih.gov)
  • The use of a protein precipitation protocol coupled to a C8-reverse phase LC-MS/MS allows for a reliable quantification of daptomycin in both plasma (in the range of 19-199 μg/mL) and breast milk (in the range of 0.12-0.32 μg/mL). (unimi.it)
  • Coadministration of efgartigimod with medications that bind to the human neonatal Fc receptor may lower systemic exposures and effectiveness of such medications. (medscape.com)
  • Closely monitor for reduced effectiveness of medications that bind to the human neonatal Fc receptor. (medscape.com)
  • This is a plasma proteomics data set derived from a laboratory controlled study simulating night shift-work. (figshare.com)
  • To the authors' knowledge this study constitutes the most complete report of the absorption of almond skin polyphenols in humans. (nuthealth.org)
  • In this study, we assess the capability of a recent plasma FIB-SEM technology which provides a potential increase in measurement speed over gallium FIB-SEM, thus paving the way to larger volume analysis. (mcmaster.ca)