Human neuroblastoma cells. Medical search. Frequent questions

Our data unravel a complex content in myeloid cells in patient tumors and further document a striking correspondence of the microenvironment populations between both mouse and human tumors. (curie.fr)
We further functionally demonstrate that MDSCs isolated from mouse neuroblastoma have immunosuppressive properties, impairing the proliferation of T lymphocytes.ConclusionsOur study demonstrates that neuroblastoma tumors have an immunocompromised microenvironment characterized by dysfunctional T cells and accumulation of immunosuppressive cells. (curie.fr)
Mutations which change amino acid residues 12, 13 or 61 activate the potential of N-ras to transform cultured cells and are implicated in a variety of human tumors e.g. melanoma. (wikipedia.org)
That finding prompted the team to take a closer look at these sequences using a modified version of circle sequencing (Circle-seq) in 21 of the neuroblastoma tumors, making it possible to enrich for circularized DNA. (genomeweb.com)
Neuroblastoma prognosis differs widely from those who bears tumors with higher frequency of spontaneous relapse and involve no intervention and those with metastatic and therapy resistant tumors (Shimada et al. (springeropen.com)
While GB tumors are frequently infiltrated by natural killer (NK) cells, these are actively suppressed by the GB cells and the GB tumor microenvironment. (frontiersin.org)
Neuroblastoma is one of the small, blue, round cell tumors of childhood. (medscape.com)
All of these tumors arise from primordial neural crest cells, which ultimately populate the sympathetic chain and the adrenal medulla. (medscape.com)
Her focus in the cancer field has been on the embryonal pediatric solid tumors neuroblastoma and Wilms tumor. (lu.se)
Resolving the Pathogenesis of Anaplastic Wilms Tumors through Spatial Mapping of Cancer Cell Evolution. (lu.se)
The authors used artificial neural croarrays is to study changes in gene ex- .nature organ the tumor arose from, how big it networks (ANNs) to identify gene-ex- pression that accompany changes in is, and what it looks like after it has pression signatures associated with spe- cell physiology, such as during develop- been imbedded in wax and colored cific subtypes of tumors. (lu.se)
0 blue-cell tumors (SRBCTs), which type, 41 (including 7 expressed often masquerade as each other. (lu.se)
The biosynthesis and storage of catecholamines in chromaffin cell tumors may differ from the biosynthesis and storage in the normal medulla. (medscape.com)

In the present paper, we show the induction of apoptosis by high doses of melatonin in the human neuroblastoma cell line SK-N-MC. (drcalapai.com)
The effects of maslinic acid on cellular apoptosis, ROS generation, cell migration and invasion, caspase activation and targeting MAPK/ERK signaling pathway were investigated. (springeropen.com)
In the present research, induction of all endogenous genetics was discovered using curcumin-mediated apoptosis in In2a neuroblastoma cells. (researchhunt.com)
Outcomes Curcumin induce apoptosis in In2a neuroblastoma cells in a dose-dependent way Shiny field pictures show curcumin-mediated In2a cells loss of life. (researchhunt.com)
Reactive oxygen species (ROS) is implicated in the progression of oxidative stress-related apoptosis and cell death of the midbrain dopaminergic neurons. (ciala.com.au)
We determined in six neuroblastoma cell lines the cytotoxicity of TPZ using DIMSCAN, a digital imaging fluorescence assay, apoptosis and mitochondrial membrane potential (ΔΨ m ) by flow cytometry, and protein expression by immunoblotting. (aacrjournals.org)
46% of cells in p53-functional cell lines but failed to cause apoptosis in p53 nonfunctional cell lines. (aacrjournals.org)
Thus, TPZ cytotoxicity for neuroblastoma cell lines in hypoxia occurred via a p53-dependent mitochondrial pathway that caused induction of p53 and p21, ΔΨ m decrease, GSH depletion, and apoptosis. (aacrjournals.org)
Capsid gene delivery into the striatum of mouse brain or interskeletal muscle resulted in cell death and inflammation, likely through capsid-induced apoptosis in vivo. (cdc.gov)
Semithin (1.0-µm) sections resulted in apoptosis and inflammation of muscle cells. (cdc.gov)
Effects on cell viability of one of the compounds, CPX, were indeed dependent on chelation of intracellular iron and mediated by both G 0 /G 1 cell cycle block and induction of apoptosis. (aspetjournals.org)
Gain and loss of function studies have indicated that miRNAs play a critical role in the regulation of all key biological functions such as development, cell proliferation, cell differentiation, and apoptosis [ 3 , 4 ]. (hindawi.com)
DNA fragmentation, caspase-3 activation, and apoptosis-related gene expression were studied in CATH.a cells. (cdc.gov)

These findings suggest that PDGF isoforms are involved not only in the promotion of the neuroblastoma cell growth, but also in neuronal cell migration, growth, and differentiation in human brain development. (jci.org)
The effects of exposure of human neuroblastoma cells to a 50 Hz magnetic field on proliferation and differentiation in 2D and 3D cultures should be investigated. (emf-portal.org)
For dopaminergic differentiation experiments, cells were treated with retinoic acid for 3 days without MF exposure , followed by treatment wirth phorbol 12-myristate 13-acetate for further 3 days in combination with MF exposure . (emf-portal.org)
Moreover, a growth arrest and a significant increase in p21, TH, DAT and GAP43 gene expression was only found in group 2 compared to the sham exposure , indicating a synergy with the differentiating agents to stimulate neuroblastoma cell differentiation into a dopaminergic phenotype . (emf-portal.org)
The authors concluded that exposure of human neuroblastoma cells to a 50 Hz magnetic field might change proliferation and differentiation in 3D cultures but not in 2D cultures . (emf-portal.org)
The effect of QA on cell survival and differentiation has therefore been examined on SH-SY5Y human neuroblastoma cells. (nih.gov)
Retinoic acid (RA, 10 μm) induced differentiation of SH-SY5Y cells into a neuronal phenotype showing neurite growth. (nih.gov)
Originally discovered as a chief regulatory protein of glucose and lipid metabolism and cell differentiation, the peroxisome proliferator-activated receptor gamma (PPARγ) is a ligand-activated transcription factor of the nuclear hormone receptor superfamily 8 . (nature.com)
Additionally, cell growth inhibition and differentiation of prostate cancer cell lines by high concentrations (micromolar to millimolar) of melatonin have been recently reported. (drcalapai.com)
Malignant transformation and maintenance of the dedifferentiated state of neural crest cells may result from failure of those cells to respond to normal signals that are responsible for normal morphologic differentiation. (medscape.com)
Her main research questions so far have dealt with tumour differentiation and cancer cell evolution. (lu.se)
Temporal multimodal single-cell profiling of native hematopoiesis illuminates altered differentiation trajectories with age. (lu.se)

[ 8 ] In 1957, Mason published a report of a child with neuroblastoma whose urine contained pressor amines. (medscape.com)
Asymptomatic reactivation of SARS-CoV-2 in a child with neuroblastoma characterised by whole genome sequencing. (cdc.gov)

In the present studies, we examined the expression of two PDGF receptor genes in human tumor cell lines derived from neural crest. (jci.org)
These Ras genes have GTP/GDP binding and GTPase activity, and their normal function may be as G-like regulatory proteins involved in the normal control of cell growth. (wikipedia.org)
Even so, the team's follow-up analyses - including RNA sequencing experiments - indicated that rearrangements stemming from extrachromosomal circular DNA from MYCN and other genes may be a recurrent and ongoing source of new mutations through a multi-hit model in neuroblastoma. (genomeweb.com)
This was made possible by the advent of single cell mRNA sequencing, a high-resolution technology that can identify different cell types present in a tissue according to the genes expressed by individual cells. (sanger.ac.uk)
miRNAs are predicted to control the activity of more than 30% of human genes [ 5 ]. (hindawi.com)
cDNA hydridization array analysis with two differing Mn concentrations and time points, identified no noteworthy mRNA inductions of genes associated with programmed cell death. (cdc.gov)
Researchers have identified several possible tumor suppressor genes in the deleted region of chromosome 1, and more research is needed to understand what role these genes play in neuroblastoma development. (medlineplus.gov)
ARNT-dependent HIF-2 transcriptional activity is not sufficient to regulate downstream target genes in neuroblastoma. (lu.se)

We report that the WNV capsid (Cp) by itself induces rapid nuclear condensation and cell death in tissue culture. (cdc.gov)

The expression of alpha and/or beta PDGF receptors was detected in a wide variety of neural crest-derived human tumor cell lines such as neuroblastoma, primitive neuroectodermal tumor, and Ewing's sarcoma by RNA blot analysis, and confirmed by immunoblot analysis. (jci.org)
Bioreductive agents, such as Tirapazamine (TPZ), are preferentially cytotoxic in hypoxia and show activity against a variety of tumor cell lines ( 1 - 4 ). (aacrjournals.org)

BackgroundHigh-risk neuroblastoma is a pediatric cancer with still a dismal prognosis, despite multimodal and intensive therapies. (curie.fr)
NEW YORK - New research suggests circularized DNA falling outside of linear chromosomes may serve as a recurrent source of somatic rearrangements in neuroblastoma, a pediatric cancer affecting immature cells in the sympathetic nervous system. (genomeweb.com)

We employed conditional translating ribosome affinity purification (TRAP) followed by RNA sequencing (TRAP-seq) for transcriptome profiling of DA neurons in transgenic Drosophila lines expressing human PARIS wild type (WT) or mutant (C571A). (nature.com)
Expression knowledge from developmental and grownup neurogenesis present relative enrichment of Notch and γ-secretase expression in stem cells, whereas expression of APP and β-secretase is enriched in neurons. (esb-itathematic.it)
However, little is known about the effects of reducing alpha-synuclein expression in human neurons. (ox.ac.uk)
We demonstrate that deletion of ERRγ from DAergic neurons in adult mice was sufficient to cause a levodopa-responsive PD-like phenotype with reductions in mitochondrial gene expression and number, that partial deficiency of ERRγ hastens synuclein-mediated toxicity, and that ERRγ overexpression reduces inclusion load and delays synuclein-mediated cell loss. (nature.com)
Our findings demonstrate that miR-339 regulates Sirt2 in human and rat neurons. (hindawi.com)

Intracellular glutathione levels and SOD1 gene expression were significantly reduced in cells exposed to the magnetic field in 3D- cultures (group 2) compared to the sham exposure , whereas no change was observed in cells grown in 2D cultures (group 1). (emf-portal.org)
NRAS is an enzyme that in humans is encoded by the NRAS gene. (wikipedia.org)
It was the third RAS gene to be discovered, and was named NRAS, for its initial identification in human neuroblastoma cells. (wikipedia.org)
Mapping the human amylase gene cluster on the proximal short arm of chromosome 1 using a highly informative (CA)n repeat" (PDF). (wikipedia.org)
He also is a member of the Center for Advanced Innate Cell Therapy at Texas Children's Cancer Center, the Center for Cell and Gene Therapy at Baylor and Texas Children's and the Dan L Duncan Comprehensive Cancer Center at Baylor. (ecancer.org)
Another important finding revealed a regulatory gene in NKT cells that may impact the efficacy of treatment. (ecancer.org)
Leveraging the multiomic platform of key collaborator Immunai, Inc., Baylor researchers found that upregulation of the BTG anti-proliferation factor 1 (BTG1) gene in infused CAR NKT cells is indicative of cell exhaustion and restricts CAR NKT cell functional activity. (ecancer.org)
It is usually extremely improper to re-express all genetics utilizing gene therapy in a range of malignancies Zarnestra and in numerous cells concurrently. (researchhunt.com)
In this study, gene expression of 19,723 cancer cells was analysed and compared to a reference of 57,972 developmental adrenal cells in the hope of identifying the cell types from which neuroblastomas arise and to find novel treatment targets. (sanger.ac.uk)
But in recent years, technological advances have sped up drug development by allowing researchers to identify differences between the biological processes, such as the expression of a particular gene, within healthy human cells and those within cancerous ones. (sanger.ac.uk)
Researchers around the world are achieving targeted gene silencing in cells that had previously been beyond the reach of conventional RNAi products due to toxicity caused by transfection reagents or undesirable viral responses. (horizondiscovery.com)
Researchers believe the deleted region could contain a gene that keeps cells from growing and dividing too quickly or in an uncontrolled way, called a tumor suppressor gene. (medlineplus.gov)

Neuroblastomas are the second most common solid tumor in children but the molecular mechanisms underlying the initiation and progression of this disease are poorly understood. (ox.ac.uk)
Our results therefore suggest that Brn-3b transcription factor contributes to proliferation of neuroblastoma cells in vivo and in vitro but may also influence progression and/or invasion during tumorigenesis. (ox.ac.uk)
Patient-derived neuroblastoma models may also provide useful information about clonal evolution, phenotypic plasticity, and cell states in relation to neuroblastoma progression. (lu.se)
The Concept of Cancer Stem Cells: Elaborating on ALDH1B1 as an Emerging Marker of Cancer Progression. (lu.se)
These ANN ment, cell-cycle progression, drug treat- with different dyes. (lu.se)

With that in mind, Henssen and colleagues from Germany, the US, and Spain profiled matched tumor and normal blood samples from 93 neuroblastoma patients using whole-genome sequencing and an algorithm that uncovers circularized DNA based on paired read orientation, uncovering preliminary evidence for complex and relatively frequent ecDNAs in neuroblastoma. (genomeweb.com)

Tumor microenvironment represents a key component of the tumor ecosystem the complexity of which has to be accurately understood to define selective targeting opportunities, including immune-based therapies.MethodsWe combined various approaches including single-cell transcriptomics to dissect the tumor microenvironment of both a transgenic mouse neuroblastoma model and a cohort of 10 biopsies from neuroblastoma patients, either at diagnosis or at relapse. (curie.fr)
Relapse of neuroblastoma commonly occurs in hypoxic tissues, and is associated with an acquired and sustained high-level drug resistance, often due to p53 loss of function. (aacrjournals.org)

Human neuroblastoma cells express alpha and beta platelet-derived growth factor receptors coupling with neurotrophic and chemotactic signaling. (jci.org)
We have also demonstrated that PDGF receptors on the human neuroblastoma cell lines were biologically functional. (jci.org)
We show that mouse intratumor T cells exhibit increased expression of inhibitory receptors at the protein level. (curie.fr)
Consistently, T cells from patients are characterized by features of exhaustion, expressing inhibitory receptors and showing low expression of effector cytokines. (curie.fr)
Altogether our data propose a novel tumor escape-mechanism based on the modulation of chemokine receptors that play pivotal roles in NK cells bone marrow homing, egress, or recruitment into peripheral tissues. (unicatt.it)
The human immunodeficiency virus type 1 (HIV-1) coat glycoprotein gp120 binds to its (co)receptors and orchestrates cell entry by the direct fusion of viral and target cell membranes. (univaq.it)
These events were triggered by a rapid increase in intracellular calcium, and in cholesterol-depleted cells engaged CXCR4 chemokine receptors. (univaq.it)
NK cells contribute to cancer immune surveillance not only by their direct natural cytotoxicity which is triggered rapidly upon stimulation through germline-encoded cell surface receptors, but also by modulating T-cell mediated antitumor immune responses through maintaining the quality of dendritic cells and enhancing the presentation of tumor antigens. (frontiersin.org)
Furthermore, similar to T cells, specific recognition and elimination of cancer cells by NK cells can be markedly enhanced through expression of chimeric antigen receptors (CARs), which provides an opportunity to generate NK-cell therapeutics of defined specificity for cancer immunotherapy. (frontiersin.org)

This innovative study by first-author Dr. Ani Grigoryan involved engineering mesenchymal stem cells and guiding them through a process mimicking how bones develop naturally in the human body. (lu.se)

We found that a 40 min overall (four times a week, 10 min each) pre-exposure to PEMF did not affect major physiological parameters and led to a significant increase of Mn-dependent superoxide dismutase activity in the human neuroblastoma SH-SY5Y cell line. (nih.gov)
1. In this study we have investigated δ and μ opioid receptor-mediated elevation of intracellular Ca 2+ concentration ([Ca 2+ ](i)) in the human neuroblastoma cell line, SH-SY5Y. (edu.au)
In this study, we showed that the presence of Abeta in a neuroblastoma cell line led to an increase in both nuclear and mitochondrial DNA damage. (cnr.it)
The intention of this research was to find out the chemical profile, cytotoxic and genotoxic results of ethanolic extracts of S. planicaulis collected in winter (leaf extract) and summer season (leaf extract and leaf + flower extract) utilizing an in vitro mannequin of human neuroblastoma cell line SH-SY5Y. (esb-itathematic.it)
In this study, we analyzed the effects of manipulating Brn-3b levels in the human neuroblastoma cell line, IMR-32 and showed that constitutive overexpression of Brn-3b consistently increased cellular growth and proliferation in monolayer as well as in an anchorage-independent manner compared with controls whereas stably decreasing Brn-3b can reduce the rate of growth of these cells. (ox.ac.uk)
Induction of p53 and p21 expression by TPZ was observed in a p53-functional cell line (SMS-SAN) but not in a p53-nonfunctional cell line (CHLA-90). (aacrjournals.org)
The PC12 human neuroblastoma cell line was used as a model for assay development. (moleculardevices.com)
This superior transfection performance allows you to reduce the reagent dose, thus helping lower toxicity risk when transfecting your cell line. (thermofisher.com)
We were very happy and surprised to see Lipofectamine® 3000 reagent provide a more than 10-fold difference in transfection efficiency in our difficult-to-transfect cell line. (thermofisher.com)
grey line histogram) or Purified Mouse Anti-Human CD171 (Cat. (bdbiosciences.com)
The 5G3 antibody was developed against a human neuroblastoma cell line to use as a probe for the elucidating the biological characteristics of neuroblastoma. (bdbiosciences.com)
In the present study, Mn toxicity was characterized in a human neuroblastoma (SK-N-SH) cell line and in a mouse catecholaminergic (CATH.a) cell line. (cdc.gov)
LINE-1 retrotransposons drive human neuronal transcriptome complexity and functional diversification. (lu.se)

For proliferation experiments, cells were exposed for 72 hours. (emf-portal.org)
Expression of the neuronal proliferation marker doublecortin revealed that, unlike RA, QA did not decrease the number of mitotic cells. (nih.gov)
Additionally, researchers found a correlation between higher antitumor activity and in vivo proliferation of infused CAR-NKT cells. (ecancer.org)
Low concentrations (nanomolar) of melatonin had been previously shown to inhibit cell proliferation in several cancer cell lines as well as in experimental animal models. (drcalapai.com)
It is possible that decreasing Brn-3b levels may reverse some effects on growth and proliferation of these cells. (ox.ac.uk)
Cancer is an unregulated proliferation of cells. (msdmanuals.com)
Hypoxia inducible factor-2α importance for migration, proliferation, and self-renewal of trunk neural crest cells. (lu.se)

A study in Nature suggests epitope editing in donor stem cells prior to bone marrow transplants can stave off toxicity when targeting acute myeloid leukemia with immunotherapy. (genomeweb.com)
Derived from a female patient with neuroblastoma from the metastasis of the bone marrow. (sigmaaldrich.com)
Red blood cell (RBC) production (erythropoiesis) takes place in the bone marrow under the control of the hormone erythropoietin (EPO). (msdmanuals.com)
Myeloproliferative neoplasms are clonal proliferations of bone marrow stem cells, which can manifest as an increased number of platelets, red blood cells (RBCs), or white blood cells (WBCs), alone or in combination, in the circulation and sometimes with fibrosis in the bone marrow and extramedullary hematopoiesis (cell production outside the marrow). (msdmanuals.com)

PDGF isoforms as well as nerve growth factor induced morphological changes showing neuronal cell maturation. (jci.org)
Although studies in normal neuronal cells would be useful to further confirm our hypothesis, we suggest that specific PEMF treatments may have potential biological repercussions in diseases where oxidative stress is implicated. (nih.gov)
Both RA and QA increased expression of the neuronal marker β3-tubulin in whole-cell homogenates and in the neuritic fraction assessed using a neurite outgrowth assay. (nih.gov)
More recently we determined that REMFS decreases toxic protein beta amyloid levels, which is the cause of Alzheimer's disease (AD), in human neuronal cultures. (intechopen.com)

Cytotoxicity of the extracts on SH-SY5Y cells was tested using MTT assay. (ciala.com.au)
Cytotoxicity results showed that crude EBN extract did not cause SH-SY5Y cell death at concentrations up to 75 μg/ml while the maximum non-toxic dose (MNTD) of water extract was double of that of crude extract. (ciala.com.au)
Nevertheless, ex vivo activation with cytokines can restore cytolytic activity of NK cells against GB, indicating that NK cells have potential for adoptive immunotherapy of GB if potent cytotoxicity can be maintained in vivo . (frontiersin.org)
Manganese -induced cytotoxicity in dopamine-producing cells. (cdc.gov)
In conclusion, DA content was not responsible for the enhanced sensitivity of CATH.a cells to Mn toxicity, but oxidative stress was implicated as a probable mechanism of cytotoxicity. (cdc.gov)

Exposure of the SH-SY5Y Human Neuroblastoma Cells to 50-Hz Magnetic Field: Comparison Between Two-Dimensional (2D) and Three-Dimensional (3D) In Vitro Cultures med. (emf-portal.org)
Finally, the analysis of NK cells from patients with stage 4 NB suggests that NB conditioning could exert in vivo an immune modulatory effect resembling that emerged from in vitro experiments. (unicatt.it)
The Brn-3b transcription factor regulates the growth, behavior, and invasiveness of human neuroblastoma cells in vitro and in vivo. (ox.ac.uk)
In vitro PD model was generated by exposing SH-SY5Y cells to neurotoxin 6-hydroxydopamine (6-OHDA). (ciala.com.au)
RD cells transfected with pcWNV-Cp-DJY or pcDNA3.1 sis in vitro through the mitochodrial/caspase-9 pathway. (cdc.gov)
Here we review recent developments in patient-derived neuroblastoma xenograft models and in vitro cultures. (lu.se)
In vitro maintenance of multipotent neural crest stem cells as crestospheres. (lu.se)

offers also been reported mainly because a pro-apoptotic proteins mediated by g75NTR13 and decreases growth development in SOD2 mouse xenograft versions of human being breasts malignancy14. (researchhunt.com)

The accumulation of cytosolic lipid globules and oxidative stress finally correlates with the apoptotic death of cells . (bvsalud.org)
Additional, enzyme-modified comet assay confirmed each oxidized purines and pyrimidines, and consequently oxidative stress was associated to genomic instability and cell demise. (esb-itathematic.it)

endothelial cells within the lining of blood veins throughout disbursement, can be advantageous. (esb-itathematic.it)
CD155 is primarily found on endothelial cells, monocytes, epithelia and central nervous system. (biolegend.com)
Microparticles Induce Cell Cycle Arrest Through Redox-Sensitive Processes in Endothelial Cells: Implications in Vascular Senescence. (ohri.ca)
Epithelioid angiosarcoma is a rare malignant neoplasm found in vascular endothelial cells. (bvsalud.org)
The histologic hallmark of AE is represented by the presence of sheets and groups of atypical endothelial cells with prominent epithelioid morphologies. (bvsalud.org)

In particular, NB cells upregulated the expression of CXCR4 and CXCR3 in all NK cells and downregulated CX(3)CR1 in the CD56(dim) subset. (unicatt.it)
Conversely, engineered reduction of BTG1 expression in CAR NKT cells enhanced their therapeutic activity against neuroblastoma in a mouse model. (ecancer.org)
Therefore, a clearer understanding capsid expression was analyzed by immunofluorescent assay of WNV is necessary in order to identify new strategies to treat in HeLa, 293-T, RD, or SH-SY5Y cells by using anti-His tag or prevent this viral infection (3). (cdc.gov)
Each reagent was used to transfect HEK 293, HeLa, LNCaP, HepG2, and A549 cell lines in a 96-well format, and GFP expression was analyzed 48 hours post-transfection. (thermofisher.com)
Flow cytometric analysis of CD171 expression on human melanoma cells. (bdbiosciences.com)
Overexpression of miR-339 downregulated Sirt2 expression, while knockdown of miR-339 upregulated Sirt2 expression in human SH-SY5Y cells and rat PC12 cells. (hindawi.com)
Enhanced expression of MycN/CIP2A drives neural crest toward a neural stem cell-like fate: Implications for priming of neuroblastoma. (lu.se)

Iron is an essential cellular nutrient, being a critical cofactor of several proteins involved in cell growth and replication. (aspetjournals.org)
Autophagy has a variety of complex physiological and pathophysiological roles, such as adaptation to nutrient starvation, clearance of damaged intracellular proteins and organelles, cell development, antiaging, elimination of microorganisms, cell death, tumor suppression, and antigen presentation. (moleculardevices.com)
Analysis of SARS-CoV-2 infection associated cell entry proteins ACE2, CD147, PPIA, and PPIB in datasets from non SARS-CoV-2 infected neuroblastoma patients, as potential prognostic and infection biomarkers in neuroblastoma. (cdc.gov)

Neuroblastoma is a harmful malignancy associated to nervous system in children (Shimada et al. (springeropen.com)
Consequently, manipulating growth cells genome by nutraceutical/h or pharmaceutic/h to re-express genetics can become of great importance in managing malignancy cells development and loss of life. (researchhunt.com)
Neuroblastoma is the most common intra-abdominal malignancy of infancy, the most common cancer in infancy, and the most common extracranial solid tumor of childhood, with an incidence of over 700 cases in the United States every year. (medscape.com)
[ 1 , 2 ] Neuroblastoma is the third most common malignancy in children up through 14 years of age, behind acute lymphocytic leukemia and cancers of the brain and central nervous system. (medscape.com)

the chromaffin cells settle near the sympathetic ganglia, the vagus nerve, paraganglia, and carotid arteries. (medscape.com)

Our work provides a new and precious data resource to better understand the neuroblastoma ecosystem and suggest novel therapeutic strategies, targeting both tumor cells and components of the microenvironment. (curie.fr)
There is thus a need for clinically relevant and authentic models of neuroblastoma that closely resemble the human disease to further interrogate underlying mechanisms and to develop novel therapeutic strategies. (lu.se)

Researchers at Baylor College of Medicine/Texas Children's Cancer Center and collaborating institutions report interim results from a first-in-human phase 1 clinical trial evaluating the safety, antitumor activity and immunological characteristics of a genetically engineered natural killer T (NKT) cell immunotherapy for neuroblastoma, a childhood tumour that most commonly arises in the adrenal gland. (ecancer.org)

We cannot always predict the extent of NKT cell expansion in vivo after infusion," said Dr. Leonid Metelitsa, co-corresponding author and professor of paediatrics - haematology and oncology at Baylor. (ecancer.org)
In this study, we found the CD62L biomarker expressed on the infused NKT cells, which we identified in our preclinical work, is predictive of both higher NKT cell in vivo expansion and antitumor activity in patients. (ecancer.org)
Here, we discuss effects of the GB tumor microenvironment on NK-cell functionality, summarize early treatment attempts with ex vivo activated NK cells, and describe relevant CAR target antigens validated with CAR-T cells. (frontiersin.org)

Because invasion and metastasis influence morbidity and mortality in neuroblastoma and so significantly affect the course and outcome of neuroblastomas, this finding is very important. (ox.ac.uk)

While past research has pointed to a role for circularized, extrachromosomal MYCN oncogene sequences in neuroblastoma, the team explained, the full suite and the frequency of somatic mutations involving small or large stretches of circularized extrachromosomal DNA amplifications had not been fully explored. (genomeweb.com)

Previous studies from the team have shown that NKT cells can localise to tumour sites in pre-clinical models. (ecancer.org)
What is most striking about our findings is that despite the great diversity of clinical behaviour of neuroblastoma, there is a patient overarching neuroblastoma cell type. (sanger.ac.uk)
These data further define the mechanism of action of TPZ and suggest that as a single agent, TPZ would only have clinical activity against p53-functional neuroblastomas. (aacrjournals.org)
We then outline preclinical approaches that employ CAR-NK cells for GB immunotherapy, and give an overview on the ongoing clinical development of ErbB2 (HER2)-specific CAR-NK cells currently applied in a phase I clinical trial in glioblastoma patients. (frontiersin.org)
The laser is now well-established as a critically enabling tool in biomedical applications that range from research and clinical work at the molecular level to direct human applications for medical conditions such as strokes. (photonics.com)

RNA interference-mediated knockdown of alpha-synuclein protects human dopaminergic neuroblastoma cells from MPP(+) toxicity and reduces dopamine transport. (ox.ac.uk)
To investigate this, we have developed a system in which levels of alpha-synuclein can be acutely suppressed by using RNA interference (RNAi) in a physiologically relevant human dopaminergic cellular model. (ox.ac.uk)

Outcomes from MTT assay display, curcumin inhibited cell expansion considerably in a dose-dependent way, g? (researchhunt.com)
Then, microscopic morphological and nuclear examination, cell viability test and ROS assay were performed to assess the protective effect of EBN extracts against 6-OHDA-induced cellular injury. (ciala.com.au)
DVTP nick-end labeling (TUNEL) assay with the In Situ Cell determined according to the manufacturer's protocol. (cdc.gov)
In human SH-SY5Y cells, the luciferase assay implied that Sirt2 was likely a target of miRNA-339. (hindawi.com)

The inducing issue was demise alerts to proliferating regular human cells (major cell strains), which respon-ded by a particular kind of tetraploidization, chromosomes with 4-chromatids (diplochromosomes, earlier described in most cancers cells). (esb-itathematic.it)
These 2n cells additionally confirmed the nuclear dependent 90° flip, which in each circumstances was related to cells gaining cell form modifications, herein illustrated from regular fibroblastic cells altering to roundness cells, indistinguishable from todays' diagnostic most cancers cell morphology. (esb-itathematic.it)
Neuroblastoma is certainly the most common youth cancers created from uncommitted sensory crest cells along the sympathetic anxious program and sometimes in central anxious program, including human brain1,2. (researchhunt.com)
It also has orphan designations for treating various cancers, including neuroblastoma . (medscape.com)

This study provides promising initial evidence of antitumor activity of GD2 CAR NKT cells against neuroblastoma. (ecancer.org)
Given the documented sensitivity of NB cells to HSP90 inhibition, we propose CPX and other iron chelators as investigational antitumor agents in NB therapy. (aspetjournals.org)

Researchers in Human Genetics and Genomics Advances report that how researchers describe genomic studies may alienate potential participants. (genomeweb.com)
Bansal (Human brain portrayed X-linked) genetics belong Zarnestra to a little family members of genetics including and in mouse while rather of in human beings. (researchhunt.com)
Furthermore, the part of any genetics is usually not really analyzed in any neuroblastoma cells. (researchhunt.com)
Consequently, we hypothesized that curcumin-mediated neuroblastoma cell loss of life might induce genetics. (researchhunt.com)
Cell signaling inhibitors had been used to investigate the feasible molecular systems behind curcumin-mediated induction of genetics and to correlate the manifestation of genetics with apoptotic neuroblastoma cells loss of life. (researchhunt.com)
Jointly, our research for the 1st period recommend that all the genetics can become caused particularly by curcumin to funnel their growth suppressor features by suppressing cell expansion and triggering apoptotic elements in In2a neuroblastoma cells. (researchhunt.com)

Here, we modulated membrane fluidity of human neuroblastoma CHP100 cells by modulating their cholesterol content, and investigated the ability of gp120 to induce cell death in comparison with the untreated cells. (univaq.it)

The intracellular calcium chelator EGTA-AM protected CHP100 cells almost completely against the toxic effects of gp120. (univaq.it)

However, gp120-induced necrosis and related biochemical changes were negligible in cholesterol-enriched, and significantly enhanced in cholesterol-depleted, CHP100 cells exposed to the viral glycoprotein under the same experimental conditions. (univaq.it)
Respiratory or enteric viral isolates obtained from PIM specimens using poliovirus-permissive cell lines are also considered PIM. (cdc.gov)

Features of related cells were validated by multicolor flow cytometry and functional assays.ResultsWe show that the immune microenvironment of MYCN-driven mouse neuroblastoma is characterized by a low content of T cells, several phenotypes of macrophages and a population of cells expressing signatures of myeloid-derived suppressor cells (MDSCs) that are molecularly distinct from the various macrophage subsets. (curie.fr)
MOXD1 is a gate-keeper of organ homeostasis and functions as a tumor-suppressor in neuroblastoma. (lu.se)

Significant ΔΨ m loss and glutathione (GSH) depletion in response to TPZ was observed in p53-functional cell lines (SMS-SAN, SMS-SAN EV, and CHLA-15) but not in p53-nonfunctional cell lines (SMS-SAN E6 and CHLA-90). (aacrjournals.org)

The study, published today (5 February 2021) in Science Advances , sought to understand why neuroblastomas range in severity, with some easy to treat and others having relatively low five-year survival rates. (sanger.ac.uk)

Cells with high Brn-3b also fail to respond to growth inhibitory retinoic acid, as they continue to proliferate. (ox.ac.uk)

[ 1 ] It is a poorly differentiated neoplasm derived from neural crest cells that typically affects infants and young children. (medscape.com)
[ 5 ] More substantial evidence of the neural origins of neuroblastoma became apparent in 1914, when Herxheimer showed that fibrils of the tumor stained positively with special neural silver stains. (medscape.com)
[ 9 ] This discovery further contributed to the understanding of neuroblastoma and its possible sympathetic neural origin. (medscape.com)
Neuroblastoma is a malignant neural tumor that typically affects very young children. (medscape.com)
Neuroblastoma is a sympathetic nervous system tumor derived from the neural crest, whereas Wilms tumour mirrors arrested kidney development. (lu.se)
Maintaining multipotent trunk neural crest stem cells as self-renewing crestospheres. (lu.se)
In the fifth week of fetal development, neuroblastic cells migrate from the thoracic neural crest to form the sympathetic chains and preaortic ganglia. (medscape.com)

In 1927, Cushing and Wolbach further characterized neuroblastoma by describing the transformation of malignant neuroblastoma into its benign counterpart, ganglioneuroma . (medscape.com)
Leukemia is a malignant condition involving the excess production of immature or abnormal leukocytes, which eventually suppresses the production of normal blood cells and results in symptoms related to cytopenias. (msdmanuals.com)

NKT cells were modified to express a GD2-specific chimeric antigen receptor (GD2 CAR), which enables the immune cells to target a molecule found on the surface of neuroblastoma cells, and interleukin-15 (IL-15), a natural protein that supports NKT cell survival. (ecancer.org)
In type 1 diabetes, the immune system destroys insulin-producing pancreatic beta cells. (medscape.com)
as well as testing in the humans showing that there didn't appear to be significant immune system modulation. (medscape.com)

Spontaneous regression of microscopic clusters of neuroblastoma cells, called neuroblastoma in situ, was noted to occur quite commonly. (medscape.com)

This treatment did not significantly affect the response of the cells to carbachol. (edu.au)
We previously showed that the Brn-3b transcription factor is highly expressed in actively proliferating neuroblastoma cells but is significantly decreased when these cells are induced to differentiate. (ox.ac.uk)
Interestingly, elevated Brn-3b levels also enhances the invasive capacity of these neuroblastoma cells with significantly larger numbers of migrating cells observed in overexpressing clones compared with controls. (ox.ac.uk)
CATH.a cells were significantly protected by the addition of 5 mM GSH (Mn EC50=200 M) and 10 mM N-acetyl cysteine (NAC) (Mn EC50=300 M), therefore, indirectly identifying intracellular ROS formation as a mechanism for Mn neurotoxicity. (cdc.gov)
Following a mixed-meal tolerance test at 6 months, the C-peptide area under the curve ― a measure of beta-cell function ― was significantly higher with the three highest DFMO doses compared to placebo ( P = .02, .03, and .02 for 125 mg/m 2 , 750 mg/m 2 , and 1000 mg/m 2 , respectively). (medscape.com)

Mefloquine binding to human acyl-CoA binding protein leads to redox stress-mediated apoptotic death of human neuroblastoma cells. (bvsalud.org)
These pictures display membrane layer blebbing (yellowish arrow) and nuclear moisture build-up or condensation (reddish arrow) just in curcumin treated cells, which are generally noticed in apoptotic cell loss of life (Fig. 1a). (researchhunt.com)
Morphological observation and nuclear staining suggested that EBN treatment reduced the level of 6-OHDA-induced apoptotic changes in SH-SY5Y cells. (ciala.com.au)
Finally, apoptotic markers of Mn-induced cell death were investigated. (cdc.gov)

Susceptibility of neuroblastoma and glioblastoma cell lines to SARS-CoV-2 infection. (cdc.gov)

In this study, we show that mefloquine binds to and inactivates the human acyl-CoA binding protein ( hACBP ), potentially inducing redox stress in human neuroblastoma cells (IMR-32). (bvsalud.org)
Each reagent was used to transfect HeLa cells in a 96-well format at the indicated doses with an emerald green fluorescent protein (GFP)-expressing vector. (thermofisher.com)

The largest single cell study to date of the childhood cancer, neuroblastoma, has answered important questions about the genesis of the disease. (sanger.ac.uk)
Neuroblastoma is an unusual cancer in that some tumours resolve without intervention, yet the disease still has one of the lowest five-year survival rates of any childhood cancer. (sanger.ac.uk)
This raises the exciting prospect that a single cell atlas of all types of paediatric tumours may transform our understanding of childhood cancer. (sanger.ac.uk)
Neuroblastoma is a childhood cancer derived from the sympathetic nervous system. (lu.se)
High-risk neuroblastoma patients have a poor overall survival and account for ~15% of childhood cancer deaths. (lu.se)

We demonstrate that the majority of genomic rearrangements in neuroblastoma involve circular DNA, challenging our current understanding about cancer genome remodeling," the authors wrote. (genomeweb.com)

Accell siRNA permits RNAi screening in difficult-to-transfect cells with no transfection reagent, virus, or electroporation. (horizondiscovery.com)
Accell siRNA achieves what no other RNAi product can claim: delivery into difficult-to-transfect cells without transfection reagents, virus, or electroporation. (horizondiscovery.com)

These deletions are somatic mutations, which means they occur during a person's lifetime and are present only in the cells that become cancerous. (medlineplus.gov)

Based on miRBase version 21 released in June 2014 ( http://www.mirbase.org/ ), there are 1,881 miRNA precursors and 2,588 mature miRNAs in humans. (hindawi.com)
These cells are believed to be the precursors of neuroblastomas and ganglioneuromas. (medscape.com)

The Laboratory of Bone Organ Modeling and Regeneration received the 2023 Article of the Year Award for their research generating human mini-bones in a laboratory setting which mimics the properties of real human bone. (lu.se)

This leads to the competitive inhibition of acyl-CoA(s) binding to hACBP and to the accumulation of lipid droplets inside the IMR-32 cells . (bvsalud.org)

Podocyte-specific overexpression of human angiotensin-converting enzyme 2 attenuates diabetic nephropathy in mice. (ohri.ca)

The researchers from the Wellcome Sanger Institute, Great Ormond Street Hospital (GOSH) and their collaborators, discovered that all neuroblastomas arise from a single type of embryonic cell called sympathoblasts. (sanger.ac.uk)
This varied outlook prompted the researchers to ask whether the range of severity could be caused by neuroblastomas arising from different cell types at different stages of the child's development in the womb. (sanger.ac.uk)
With the Human Accell Phosphatases siRNA library, researchers receive siRNAs targeting enzymes with known or predicted phosphatase activity. (horizondiscovery.com)
In today's world of science, single-cell sequencing technologies allow researchers to examine the genetic details of individual cells, creating a treasure trove of data. (lu.se)

Autophagy: renovation of cells and tissues. (moleculardevices.com)
But for the most part, normal tissues and 9 cell lines). (lu.se)

According to Beckwith and Perrin in 1963, regression occurs nearly 40 times more often than clinically apparent neuroblastoma. (medscape.com)

Notably, a similar receptor modulation was observed in rTGF-beta 2-conditioned NK cells. (unicatt.it)

We also generated genome-wide maps of PARIS occupancy using ChIP-seq in human SH-SY5Y cells. (nature.com)
The response included cell cycle stress, which extended S-period with results of mitotic slippage course of, forming the particular 4n cells by re-replication of diploid cells, which confirmed cell division functionality to sudden, genome diminished diploid cells which remarkably, confirmed health acquire. (esb-itathematic.it)
The nucleus turned, self-inflicted, 90° perpendicular to the cell's cytoskeleton axis, importantly, earlier than the particular 4n-division system produced genome scale back diploid cells, we name "first cells", due to health acquire. (esb-itathematic.it)

Compared with normal cells, neoplastic cells have been shown to require a greater amount of iron, thus laying the basis for the promising anticancer activity of iron chelators. (aspetjournals.org)

Anatomy13

Organisms4

Diseases9

Chemicals and Drugs82

Analytical, Diagnostic and Therapeutic Techniques and Equipment14

Psychiatry and Psychology1

Phenomena and Processes35

Disciplines and Occupations1

Information Science3

Tumors13

Apoptosis13

Differentiation12

Child with neuroblastoma2

Genes8

Induces1

Tumor cell lines2

Pediatric2

Neurons5

Gene12

Progression5

Whole-genome1

Relapse2

Receptors9

Mesenchymal stem1

Line13

Proliferation7

Bone marrow4

Neuronal4

Cytotoxicity5

Vitro7

Xenograft1

Oxidative2

Endothelial cells5

Expression7

Proteins3

Malignancy4

Sympathetic ganglia1

Therapeutic2

Adrenal1

Vivo3

Metastasis1

Oncogene1

Clinical5

Dopaminergic2

Assay4

Cancers4

Antitumor2

Genetics6

Induce1

Intracellular calcium1

Viral2

Suppressor2

Functional1

20211

Retinoic1

Neural7

Malignant2

Immune3

Commonly1

Significantly5

Apoptotic4

Glioblastoma1

Protein2

Childhood5

Genomic1

Difficult-to-transfect cells2

Somatic1

Precursors2

20231

Inhibition1

Overexpression1

Researchers4

Tissues2

Clinically1

Modulation1

Genome3

Neoplastic1