Human lung epithelial cells. Medical search. Frequent questions

We performed epigenomic profiling in conjunction with transcriptomic profiling using in vitro differentiation of human primary alveolar epithelial cells (AEC). (huji.ac.il)
The 3dGRO™ Human Lung Organoid Culture System is a serum-free, multi-stage culture system for efficient differentiation of human induced pluripotent stem (iPS) cells to mature lung organoids that structurally resemble the in vivo branching airway and early alveolar structures. (sigmaaldrich.com)
AFE cells may be cryopreserved (SCC301) or further differentiated into branching lung bud organoids using 3dGRO™ Lung Organoid Branching Medium (SCM307) and further matured into branching and alveolar lung organoids using 3dGRO™ Lung Organoid Maturation Medium (SCM308). (sigmaaldrich.com)
SARS-CoV-2 infection induces Ace2 gene and its interacting factors in human alveolar but not bronchial epithelial cells. (biomedcentral.com)
Human bronchial epithelial cells (HBEC) and alveolar epithelial (A549) cells were infected with SARS-CoV-2 for 24 h, and control cells received medium only. (biomedcentral.com)
The mRNA expression of ACE2 ( a ), TMPRSS2 ( b ), ADAM17 ( c ), TIMP3 ( d ), AGF ( e ), TGFB1 ( f ), CTGF ( g ), VEGFA ( h ) and FN1 ( i ) in HBECs and alveolar epithelia cells. (biomedcentral.com)
Surfactant is produced by alveolar type II cells which can be differentiated in vitro from patient specific induced pluripotent stem cell (iPSC)-derived lung organoids. (nature.com)
Lung development is a complicated process that involves the specialization of multiple bronchiolar and alveolar epithelial populations. (nature.com)
Alveolar type II (ATII) epithelial cells synthesize, secrete and recycle all components of surfactant and dysfunction in surfactant metabolism can result in a variety of pediatric lung diseases including respiratory distress syndrome and interstitial lung disease 3 . (nature.com)
The lung alveolar epithelium represents a major site of tissue injury in IPF and senescence of this cell population is probably detrimental to lung repair. (ersjournals.com)
However, the potential pathomechanisms of alveolar epithelial cell senescence and the impact of senolytic drugs on senescent lung cells and fibrosis remain unknown. (ersjournals.com)
Primary fibrotic mouse alveolar epithelial type (AT)II cells secreted increased amounts of senescence-associated secretory phenotype (SASP) factors in vitro , as analysed using quantitative PCR, mass spectrometry and ELISA. (ersjournals.com)
Lung epithelial cells form a barrier that lines airway and alveolar surfaces and constitute an air-liquid interface. (springer.com)
Discussion: In conclusion, our data indicate that bronchial and alveolar epithelial cells respond differently to hypoxia and profibrotic stimuli. (lu.se)
Acute lung injury (ALI) or acute respiratory distress syndrome (ARDS) is associated with acute and diffuse alveolar damage, noncardiogenic pulmonary oedema and subsequent pulmonary fibrosis. (ersjournals.com)
CHD6, a cellular repressor of influenza virus replication, is degraded in human alveolar epithelial cells and mice lungs during infection. (nih.gov)
Oxidant stress from smoking may damage alveolar epithelial cells and contribute to the pathogenesis of idiopathic pulmonary fibrosis. (medscape.com)
Victoria Ptasinski defended her Ph.D. thesis 'Alveolar Progenitor Cells in Lung Damage and Regeneration in Pulmonary Fibrosis' on Monday, November 27, 2023. (lu.se)

Here, we show that benzo[a]pyrene diol epoxide (BPDE, the active metabolite of cigarette smoke carcinogen benzo[a]pyrene)-induced human bronchial epithelial cell (HBEC) transformation was enhanced by IL-6 in vitro. (psu.edu)
The lungs have a unique blood supply, receiving deoxygenated blood from the heart in the pulmonary circulation for the purposes of receiving oxygen and releasing carbon dioxide, and a separate supply of oxygenated blood to the tissue of the lungs, in the bronchial circulation. (wikipedia.org)
Herein we elaborated effects of TGF-β1 on TJs of primary human bronchial epithelial cells. (springer.com)
The bronchial epithelium appears more responsive to changes in oxygen levels and remodelling processes compared to the alveoli, suggesting that hypoxia may be a driver of pathogenesis in chronic lung disorders. (lu.se)
This occurs because of the inflammatory response of the mucous membranes within the lungs' bronchial passages. (medscape.com)
In human lung tissue TGF-β 1 produced by bronchial epithelial cells stimulates fibroblasts to proliferate in vitro. (bmj.com)
They exposed normal human bronchial epithelial (NHBE) cells to the chemicals for 24 hours. (newswise.com)

We recently investigated and compared the cellular gene expression profiles of human lung A549 cells infected by five different subtypes of human and avian influenza viruses (Josset et al. (nih.gov)
It was demonstrated that LL-37 was actively taken up into A549 epithelial cells and eventually localized to the perinuclear region. (ed.ac.uk)
By using nonlinear regression analysis, it was revealed that A549 epithelial cells have two receptors for LL-37B, with high and low affinity for LL-37, respectively. (ed.ac.uk)
Using an electrostatic field-based air-liquid exposure (ALI) chamber, the two shapes were exposed to a co-culture of human airway epithelial (A549) and differentiated human monocyte (THP-1) cells at ALI conditions. (aiche.org)
A549-ASCoV2 NLRP1 cells and their control cell line overexpress the genes encoding the SARS-CoV-2 human receptors, ACE2 and TMPRSS2 [1], and are thus highly permissive to SARS-CoV-2 infection. (invivogen.com)
A549-ASCoV2-NLRP1 cells have been additionally engineered to stably express the human NLRP1 inflammasome sensor. (invivogen.com)
GFP with the NF-κB inducer TNF-α and stimulation using SARS-CoV-2 infectious particles, A549-ASCoV2-NLRP1 cells display ASC specks, visible as fluorescent green dots and undergo pyroptosis, readily measurable by the LDH assay. (invivogen.com)
A549-ASCoV2 cells not only serve as a control cell line for A549-ASCoV2-NLRP1 cells but also offer the possibility to monitor ASC-dependent inflammasome activation of NLRP3 after its transgenic expression. (invivogen.com)
Surface expression of hACE2 by A549-ASC, A549-ASCoV2, A549-ASCoV2-NLRP1 cells. (invivogen.com)
Lysates from A549-ASCoV2 (1) and A549-ASCoV2-NLRP1 (2) cells were analyzed by western blot using an anti‑human NLRP1 and an anti-human ASC antibody, followed by HRP conjugated secondary antibody. (invivogen.com)
Estradiol reduces ACE2 and TMPRSS2 mRNA levels in A549 human lung epithelial cells. (cdc.gov)

Lung organoids are useful three-dimensional (3D) cell culture models for studying human lung development and respiratory diseases including viral infections (SARS-CoV, H1N1, MERS), cystic fibrosis, asthma/COPD, exposure to air pollution and the effects of smoking. (sigmaaldrich.com)
Idiopathic pulmonary fibrosis (IPF) is a devastating lung disease with poor prognosis and limited therapeutic options. (ersjournals.com)
Here we demonstrate that lung epithelial cells exhibit increased P16 and P21 expression as well as senescence-associated β-galactosidase activity in experimental and human lung fibrosis tissue and primary cells. (ersjournals.com)
It is believed that TGF-β1 signalling is involved in controlling epithelial cell proliferation, to drive epithelial-mesenchymal transition (EMT) and it plays a major role in lung fibrosis and emphysema. (springer.com)
In the bleomycin-induced early-stage pulmonary fibrosis model, the neutrophil elastase level was increased in the lungs. (ersjournals.com)
Sivelestat significantly inhibited the increase in lung collagen content, fibrotic changes, the numbers of total cells (including macrophages, neutrophils and lymphocytes), the levels of the active form of TGF-β1 and phospho-Smad2 in bleomycin-induced early-stage pulmonary fibrosis. (ersjournals.com)
These results suggest that sivelestat alleviated bleomycin-induced pulmonary fibrosis via inhibition of both TGF-β activation and inflammatory cell recruitment in the lung. (ersjournals.com)
Pulmonary fibrosis is caused by a variety of insults to the lung, such as acute lung injury, irradiation and drugs [ 1 ]. (ersjournals.com)
Transforming growth factor (TGF)-β, especially its isoform TGF-β1, is a profibrotic cytokine that is overexpressed in patients with fibrosis and was directly associated with fibrosis in multiple human settings and animal models [ 5 ]. (ersjournals.com)
RESULTS Patients with cystic fibrosis of a TGF-β 1 high producer genotype for codon 10 had more rapid deterioration in lung function than those with a TGF-β 1 low producer genotype. (bmj.com)
9 Previous animal and human studies have shown that high TGF-β 1 producers develop significantly more lung fibrosis in response to a number of inflammatory triggers such as radiation, 10 chemotherapy, 11 and lung transplantation. (bmj.com)
Examination of lung tissue in two patients with cystic fibrosis showed strong immunohistological staining of inflammatory tissue using an antibody against this cytokine. (bmj.com)
The authors discuss evidence suggesting that embryonic signaling pathways involved in epithelium/mesenchymal communication and epithelial cell plasticity may be aberrantly switched on in idiopathic pulmonary fibrosis. (nih.gov)
Among asymptomatic patients with idiopathic pulmonary fibrosis (diagnosed by radiographic abnormalities found on routine chest radiograph screening and lung biopsy showing usual interstitial pneumonia), symptoms developed approximately 1000 days after the recognition of the radiographic abnormality. (medscape.com)
Pulmonary hypertension is a common comorbidity in patients with idiopathic pulmonary fibrosis, and an estimated 20-40% of patients with idiopathic pulmonary fibrosis who are evaluated or listed for lung transplantation have pulmonary hypertension at rest. (medscape.com)
Her academic journey has been driven by a burning interest to understand what she considers to be the biggest mystery in this world, the human lung, particularly concerning diseases such as idiopathic pulmonary fibrosis (IPF). (lu.se)
My focus has been on a specific lung disease called idiopathic pulmonary fibrosis (IPF). (lu.se)

Here we examined how epigenetic changes are integrated with transcriptional activation to determine cell phenotype during differentiation. (huji.ac.il)
Human lung organoid differentiation workflow. (sigmaaldrich.com)
Here we show the differentiation of patient specific iPSCs derived from a patient with SFTPB deficiency into lung organoids with mesenchymal and epithelial cell populations from both the proximal and distal portions of the human lung. (nature.com)
After differentiating the mutant and corrected cells into lung organoids, we show expression of SFTPB mRNA during endodermal and organoid differentiation but the protein product only after organoid differentiation. (nature.com)
Although many differentiation protocols in the literature have been successful in mimicking lung development from stem cells, there has not been an examination of how a specific mutation impacts the differentiation process including its effects on the early endoderm, as well as the proximal and distal lung epithelial cell populations in the lung organoids. (nature.com)
The authors sought to identify mechanisms driving the differentiation of mucus cells during the bronchiolisation process. (bmj.com)
Methods Pathways governing airway mucus cell differentiation include SRY (sex determining region Y)-box 2 (SOX2), Notch, forkhead box A3(FOXA3)/SAM pointed domain containing ETS transcription factor (SPDEF), epidermal growth factor (EGF) and the EGF-related neuregulins NRG1α and NRG1β. (bmj.com)
Results In IPF, the bronchiolised and enlarged distal airspaces stained for SOX2 are consistent with epithelial differentiation characteristic of conducting airway epithelium. (bmj.com)
In this work, pathways governing mucus cell differentiation were investigated in lung tissues from patients with IPF and other chronic pulmonary disorders. (bmj.com)
Neuregulin1α, which drives mucus cell differentiation in vitro, was expressed in normal airway submucosal glands and in lungs from patients with IPF. (bmj.com)
Activation of abnormal respiratory epithelial differentiation programs may contribute to the expression of MUC5B and bronchiolisation of the distal lung, a salient feature of IPF. (bmj.com)
The proliferation and differentiation of satellite cells are regulated by a number of extracellular signals ( Wang & Rudnicki, 2012 Wang YX, Rudnicki MA. (scielo.br)
Besides its essential role in vision, vitamin A is also important in cellular differentiation (eg, growth, reproduction, immune response) and in maintenance of epithelial integrity. (medscape.com)
The cycling between the major storage organs, such as the liver, and epithelial tissues that require vitamin A for cellular differentiation is extensive and efficient. (medscape.com)

Here we show that the T-box transcription factor Brachyury induces in tumor cells epithelial-mesenchymal transition (EMT), an important step in the progression of primary tumors toward metastasis. (jci.org)
Overexpression of Brachyury in human carcinoma cells induced changes characteristic of EMT, including upregulation of mesenchymal markers, downregulation of epithelial markers, and an increase in cell migration and invasion. (jci.org)
Conversely, inhibition of Brachyury resulted in downregulation of mesenchymal markers and loss of cell migration and invasion and diminished the ability of human tumor cells to form lung metastases in a xenograft model. (jci.org)
During embryonic stages of lung organogenesis, TGF-β1 is pivotal in epithelial-mesenchymal interaction, during alveolarization and for airway branching morphology. (springer.com)

Constitutive STAT3 activation drove cell proliferation and survival through anti-apoptosis gene expression. (psu.edu)
However, isolated reports showed that user-tailoring could induce deleterious effects in exposed cells, from decrease in cellular proliferation, to changes in cellular adhesion, generation of reactive oxygen species or phenotypical variations, just to name a few. (cdc.gov)
TGF-β 1 is produced by many cells throughout the body and promotes the proliferation of fibroblasts and the deposition of collagen. (bmj.com)
The altered gene expression may enhance several cancer-related events such as cell division (proliferation), cell movement, and the development of new blood vessels (angiogenesis) that nourish a growing tumor. (medlineplus.gov)

NRG1α may contribute to bronchiolisation of the distal lung seen in IPF. (bmj.com)
The Omicron S-bearing virus robustly escapes vaccine-induced humoral immunity, mainly due to mutations in the receptor-binding motif (RBM), yet unlike naturally occurring Omicron, efficiently replicates in cell lines and primary-like distal lung cells. (biorxiv.org)

We further show that quercetin, a dietary compound having preventive properties for lung cancer, decreased BPDE-stimulated IL-6 secretion from human lung fibroblasts through inhibition of the NF-κB and ERK pathways. (psu.edu)

Unlike traditional immortalized pulmonary cell lines and primary cells, lung organoids contain various differentiated cell types with complex tissue architecture that more closely resemble in vivo tissues and functionality. (sigmaaldrich.com)
Real-time PCR was performed for Brachyury on lung tumor tissue cDNA from 80 lung cancer patients of the indicated stages of disease. (jci.org)
The interaction of LL-37 with epithelial cells was characterized in tissue culture by using biotinylated LL-37 and confocal microscopy. (ed.ac.uk)
After fulfilling all diagnostic requirements, fresh non- tumour residual lung tissue was used for this study. (cdc.gov)
Supernatant was removed and the cell pellet was resuspended in a 1:1 mixture of DMEM/F12 medium and small airway growth medium (SAGM) (Lonza) supplemented with 5% FBS and 350U/ml DNase I. The cell suspension was seeded to tissue culture flasks (Corning) and incubated in a humidified incubator at 37oC and 5% CO2 for 90 min. (cdc.gov)
The tissue of the lungs can be affected by a number of respiratory diseases, including pneumonia and lung cancer. (wikipedia.org)
Introduction: Chronic lung disorders involve pathological alterations in the lung tissue with hypoxia as a consequence. (lu.se)
Immunostaining for components of those pathways and mucins were performed on lung tissue obtained from patients with IPF (n=20), chronic obstructive pulmonary disease (n=13), idiopathic pulmonary artery hypertension (n=5) and from organ donors (n=6). (bmj.com)
Dissociating cells from specific tissue types requires specific parameters for tissue agitation to obtain a high volume of viable, culturable cells. (jove.com)
In this publication the use of this apparatus on lung tissue is explained. (jove.com)
In IPF, the lung loses that ability as it becomes filled with other kinds of tissue that make it very stiff. (lu.se)
If a lung or thyroid cancer invades the airway, surgery is sometimes still feasible if assessment indicates sufficient tissue is available for airway reconstruction. (msdmanuals.com)

In the new study, researchers used novel lab techniques that allowed them to examine the impact of both diacetyl and 2,3-pentanedione on epithelial cells in a system that closely mimicked the human airway epithelium in vivo. (newswise.com)

Bober E, Franz T, Arnold HH, Gruss P, Tremblay P. Pax-3 is required for the development of limb muscles:a possible role for the migration of dermomyotomal muscle progenitor cells. (scielo.br)

In the current study, we collected high-throughput gene expression data related to human lung epithelial cells infected with SARS-CoV-2 or other respiratory viruses (SARS, H1N1, rhinovirus, avian influenza, and Dhori) and compared the effect of these viruses on the human transcriptome. (biorxiv.org)
these included CSF2 (colony-stimulating factor 2) and S100A8 and S100A9 (calcium-binding proteins), all of which are involved in lung/respiratory disorders. (biorxiv.org)
The lungs are the primary organs of the respiratory system in humans and most other animals, including some snails and a small number of fish. (wikipedia.org)
The lungs are part of the lower respiratory tract that begins at the trachea and branches into the bronchi and bronchioles, and which receive air breathed in via the conducting zone. (wikipedia.org)
It is critical to obtain a complete history, including medication history, drug use, social history, occupational, recreational, and environmental respiratory exposure history, risk factors for human immunodeficiency virus infection, and review of systems, to ensure other causes of interstitial lung disease are excluded. (medscape.com)

Additionally, lung organoids can be derived from small amounts of patient tissues or pluripotent stem cells to create living biobanks that facilitate personalized biomedical research. (sigmaaldrich.com)
Furthermore, we found Brachyury to be overexpressed in various human tumor tissues and tumor cell lines compared with normal tissues. (jci.org)
We also determined that the percentage of human lung tumor tissues positive for Brachyury expression increased with the stage of the tumor, indicating a potential association between Brachyury and tumor progression. (jci.org)
Brachyury expression in human lung tumor tissues. (jci.org)
Human lung tissues were removed from patients undergoing surgical resection as part of a routine clinical care in Hong Kong. (cdc.gov)
Human lung tissues were minced and washed with AdDF+++ basic medium, which consisted of Advanced DMEM/F-12 (Gibco) supplemented with 1x GlutaMAXTM (Gibco), 10 mM HEPES (Gibco), and 100 U/ml Penicillin-Streptomycin (Gibco). (cdc.gov)
Human lung tissues were minced and washed 3 times using HBSS (Gibco) supplemented with 0.7 mM sodium bicarbonate (Gibco) at pH 7.4. (cdc.gov)
The scope of the special issue is to summarize and enlarge the knowledge in signalling processes and networks in diverse cells and tissues. (mdpi.com)
To determine their possible roles in tissues and satellite cells in vitro, their expression pattern was examined in tissues from 40-day-old chickens and in satellite cells from the breast muscles of 1-week-old and 2-week-old chickens using RT-PCR and immunocytochemistry analyses. (scielo.br)

Heparan sulfate proteoglycans (HSPGs) are present on the cell surface and in the extracellular matrix in all metazoans. (scielo.br)

In embryonic development, the lungs begin to develop as an outpouching of the foregut, a tube which goes on to form the upper part of the digestive system. (wikipedia.org)
Gros J, Manceau M, Thomé V, Marcelle C. A common somitic origin for embryonic muscle progenitors and satellite cells. (scielo.br)

Thus, human primary macr. (researchgate.net)
Mucociliary clearance is an important primary innate defense mechanism that protects the lungs from the harmful effects of inhaled pollutants, allergens, and pathogens. (medscape.com)
Upon increasing culture times in chicken´s primary skeletal muscle satellite cells, SULF1 and SULF2 expression in 1-week-old chickens was significantly higher than in 2-week-old chickens, suggesting that sulfatases play a key role in satellite cell development. (scielo.br)

Dr. Pryhuber trained as a post-doctoral Proctor Research Scholar and participant in the University of Cincinnati Program of Excellence in Molecular Biology of the Heart and Lung. (rochester.edu)
She serves on NIH study sections and as reviewer for journals in her area of expertise, manages an active laboratory, supervises students in pulmonary biology and epithelial cell research and has received awards recognizing both teaching and original research accomplishments. (rochester.edu)
Nature Reviews Molecular Cell Biology 2012;13(2):127-33. (scielo.br)
My laboratory generates preclinical models of lung cancer and employs diverse disciplines such as molecular biology, clinical oncology, immunology and metabolomics to prioritise clinically relevant questions. (edu.au)
More recently, his group invented tools for cleavage-free large gene insertion via mining microbial recombination protein, and developed single-cell tracking approach for studying cancer biology and cancer immunology. (stanford.edu)

This model recapitulates an in vivo process in which AEC transition from one differentiated cell type to another during regeneration following lung injury. (huji.ac.il)
Although ex vivo lung perfusion (EVLP) is able to recover marginal quality donor lungs, extension of normothermic support beyond 6 h has been challenging. (nature.com)
Normothermic ex vivo lung perfusion in clinical lung transplantation. (nature.com)

STARD12 co-expressed genes participated in cell cycle and DNA replication, and STARD14 were enriched in ECM-receptor interaction. (medsci.org)

Together, the lungs contain approximately 2,400 kilometres (1,500 miles) of airways and 300 to 500 million alveoli. (wikipedia.org)
Mucus cells lining abnormal airways from patients with IPF expressed MUC5B and SOX2 but lacked SAM pointed domain containing ETS transcription factor (SPDEF) and forkhead box A3(FOXA3). (bmj.com)
In the lab, the researchers showed that the virus was able to infect cultures of human epithelial cells that line the airways of the lungs. (medicalnewstoday.com)
Cilia are antennae-like protuberances that are present on 50-75% of the cells that line human airways. (newswise.com)
Other tumors (eg, thyroid, lung) can locally invade the airways. (msdmanuals.com)

LL-37 is a human cationic host defense peptide that is an essential component of innate immunity. (ed.ac.uk)
In addition to its modest antimicrobial activity, LL-37 affects the gene expression and behavior of effector cells involved in the innate immune response, although its mode of interaction with eukaryotic cells remains unclear. (ed.ac.uk)
These results indicate the mode of interaction of LL-37 with epithelial cells and further our understanding of its role in modulating the innate immune response. (ed.ac.uk)
Aroylated phenylenediamines (APDs) are novel modulators of innate immunity with respect to enhancing the expression of antimicrobial peptides and maintaining epithelial barrier integrity. (researchgate.net)

These findings suggest that a lethal lung disease can be targeted and corrected in a human lung organoid model in vitro . (nature.com)

Dichloromethane (DCM) is possibly carcinogenic to human s (Group 2B) based on sufficient evidence in experimental animals [lung and liver tumors in mice exposed by inhalation, and mammary tumors in rats (both sexes) exposed by inhalation]. (who.int)
Pleomorphic adenomas and granular cell and benign cartilaginous tumors also occur. (msdmanuals.com)

They are 3-dimensional, complex, multicellular structures that can be derived from patient specific human induced pluripotent stem cells (hiPSCs). (nature.com)

Inducible depletion of satellite cells in adult, sedentary mice impairs muscle regenerative capacity without affecting sarcopenia. (scielo.br)

By identifying changes in active hallmarks involved in cell-cell connections and maintaining epithelial layer integrity, we also determine the role that short-term exposure to SWCNTs plays in the overall cellular fate and cellular transformation. (cdc.gov)
This triggered an increase in paracellular permeability via cellular redistribution of claudin 3 (CLDN3) from TJs into cell nuclei followed by disruption of epithelial integrity and formation of epithelial lesions. (springer.com)
We conclude that the observed effects contribute to loss of epithelial integrity during atopic asthma. (springer.com)
No nutritional deficiency is more synergistic with infection than vitamin A. The 2 main mechanisms involved in the prevention of disease are the effect of vitamin A on the immune system and on epithelial integrity. (medscape.com)

Elucidation of the epigenetic basis for cell-type specific gene regulation is key to gaining a full understanding of how the distinct phenotypes of differentiated cells are achieved and maintained. (huji.ac.il)
Gene therapy has been utilized successfully to repair or inactivate mutations in animal models of monogenic human diseases 19 as well as human in vitro cells 12 . (nature.com)
For the same mass dose of 2 mg/cm 2 , both shapes were ingested by cells, and induced a pro-inflammatory response (IL-8 and TNFÎ±) and enhanced CYP1A1 gene expression compared to air controls. (aiche.org)
RNAi-Mediated Targeting of Noncoding and Coding Sequences in DNA Repair Gene Messages Efficiently Radiosensitizes Human Tumor Cells. (nih.gov)
Our new study suggests that these chemicals may be harming cilia--the first line of defense in the lungs--by altering gene expression related to cilia production and function," said Quan Lu, associate professor of environmental genetics and pathophysiology. (newswise.com)
The SEPTIN9 gene seems to be turned on (active) in cells throughout the body. (medlineplus.gov)

SOX2 is the determining oncogenic switch in promoting lung squamous cell carcinoma from different cells of origin. (edu.au)
Hemoptysis, while uncommon, more often occurs with a squamous cell carcinoma and can potentially lead to earlier diagnosis, whereas wheezing or stridor occurs more often with the adenoid cystic variant. (msdmanuals.com)

In their paper, the scientists warn that existing flu vaccines are unlikely to protect human populations from G4. (medicalnewstoday.com)
We also have the ability to direct cancer-associated mutations to specific lung epithelial cell populations, which enables us to interrogate potential cells-of-origin. (edu.au)

A down-regulation of mRNA expression was observed for the main regulators of p53 protein stability during infection by the complete set of viruses tested, and a significant decrease in p53 mRNA expression was also observed in H5N1 infected cells. (nih.gov)
Total mRNA was extracted from ~1x10 6 cells for each cell line. (invivogen.com)

After 14 days in culture at 37oC and 5% CO2, human airway organoids were ready for infection. (cdc.gov)
Although the treatment is associated with long term B-cells depletion, patient doesn´t suffer from any clinically relevant infection. (nel.edu)
Infection of iPSCs with lentiviral inserts is a highly efficient process since stem cells grow quickly, remain undifferentiated in specific cell culture conditions and can establish fully infected clones within 2-3 passages. (nature.com)
Upon SARS-CoV-2 infection, viral RNA is released in target cells and immediately translated to produce viral polyprotein including the 3CL protease (NSP5). (invivogen.com)

Its activation can be monitored in real-time by following the formation of fluorescent ASC specks and measuring pyroptotic cell death with the LDH assay. (invivogen.com)
subintegrum , Decreases Inflammatory Response by Inhibiting PKCÎ´ Activation in Human Lung Epithelial Cells. (bvsalud.org)
Therapeutic application of estrogen for COVID-19: Attenuation of SARS-CoV-2 spike protein and IL-6 stimulated, ACE2-dependent NOX2 activation, ROS production and MCP-1 upregulation in endothelial cells. (cdc.gov)

2019). Distinct initiating events underpin the immune and metabolic heterogeneity of KRAS-mutant lung adenocarcinoma. (edu.au)

Researchers in China have identified an influenza virus called G4 that can infect both pigs and humans. (medicalnewstoday.com)
Research led by Honglei Sun at China Agricultural University (CAU) in Beijing has identified such a strain in pigs that has already begun to infect humans. (medicalnewstoday.com)
The virus also had the ability to infect ferrets, which researchers often use to model human influenza, and to transmit from animal to animal via tiny airborne droplets called aerosols. (medicalnewstoday.com)
Cyclospora and Cryptosporidium are examples of coccidian parasites that infect the epithelial (lining) cells of the intestinal tract. (cdc.gov)

Patients awaiting lung transplantation face high wait-list mortality, as injury precludes the use of most donor lungs. (nature.com)
Here we demonstrate that acutely injured human lungs declined for transplantation, including a lung that failed to recover on EVLP, can be recovered by cross-circulation of whole blood between explanted human lungs and a Yorkshire swine. (nature.com)
Our findings suggest that cross-circulation can serve as a complementary approach to clinical EVLP to recover injured donor lungs that could not otherwise be utilized for transplantation, as well as a translational research platform for immunomodulation and advanced organ bioengineering. (nature.com)

Metastatic disease is responsible for the majority of human cancer deaths. (jci.org)
The selective expression of Brachyury in tumor cells and its role in EMT and cancer progression suggest that Brachyury may be an attractive target for antitumor therapies. (jci.org)
As controls, 16 samples of normal lung cDNA were also analyzed, each obtained from a pathologically normal section of lung from a cancer patient. (jci.org)
As an emerging cancer immunotherapy, oncolytic virotherapies (OVTs) can not only selectively lyse cancer cells, but also induce a systemic antitumor immune response. (frontiersin.org)
Gastric cancer (GC) is a common malignant tumor originating from gastric mucosa epithelial cells, and its onset is relatively insidious. (frontiersin.org)
Unfortunately, patients are already in the terminal stage at this time, and the cancer cells have already invaded the surrounding organs or metastasized far away, which leads to the loss of the curative chance for most patients ( 1 ). (frontiersin.org)
Lung cancer is the first leading diagnosed malignant tumor globally [ 1 ]. (medsci.org)
Non-small cell lung cancer (NSCLC) accounts for 85-90% of cases, while lung adenocarcinoma (LUAD) is the most common subtype [ 2 ]. (medsci.org)
One major interest is the development of novel preclinical models that closely resemble human lung cancer. (edu.au)
Genetic alterations found in human lung cancer patients can be replicated in our models using Cre-LoxP recombination and CRISPR/Cas9 technology. (edu.au)
Through flow cytometric studies we have developed methods to interrogate the immune microenvironment of lung cancer. (edu.au)
We are interested in understanding mechanisms of immunosurveillance with the aim of exploiting this knowledge to harness the immune system to treat lung cancer. (edu.au)
We are also interested in tumour heterogeneity and how this heterogeneity influences metastatic dissemination in lung cancer. (edu.au)
Cancer Cell. (edu.au)
2018). Synergy between the KEAP1/NRF2 and PI3K pathway drives non-small cell lung cancer with an altered immune microenvironment. (edu.au)
Cancer Cell 30(4):519-532. (edu.au)
1999), renal cell cancer (Dosemeci et al. (who.int)
High SEPT9_v1 expression in human breast cancer cells is associated with oncogenic phenotypes. (medlineplus.gov)
Стадіювання Lung carcinoma is the leading cause of cancer-related death worldwide. (msdmanuals.com)

p class=\'abstract\'>There is a large global unmet need for the development of countermeasures to combat hundreds of viruses known to cause human disease and for the establishment of a therapeutic portfolio for future pandemic preparedness. (stanford.edu)

A number of occupational lung diseases can be caused by substances such as coal dust, asbestos fibres, and crystalline silica dust. (wikipedia.org)
Impaired cilia function has been linked with lung diseases such as chronic obstructive pulmonary disease (COPD) and asthma. (newswise.com)
It is of interest to us all to understand how they work and how we can develop new treatments for diseases that target the lungs. (lu.se)
I have studied reasons why the lung develops diseases that are currently not curable and why there is damage occurring in the lung that does not seem to stop but progresses into the development of a disease that we die from. (lu.se)

In addition, it requested submission of full discriptions of the lung lesions seen in the new long-term rat study and of historical control data on all lung lesions in the strain of rats utilized in the study in the laboratory in which it was conducted. (inchem.org)

Dr. Pryhuber's particular clinical interests are care of infants with pulmonary hypertension, lung injury, growth and developmental failure, often complicated by chronic and inflammatory disease. (rochester.edu)

2. The aim of this work was to investigate how hypoxia affects human lung epithelial cells in combination with profibrotic stimuli and its correlation to pathogenesis. (lu.se)
Metabolic activity was significantly increased by hypoxia in both epithelial cell types. (lu.se)

Potent inhibition of human tyrosinase inhibitor by verproside from the whole plant of Pseudolysimachion rotundum var. (bvsalud.org)

Our study provides a comprehensive analysis of the synergistic cyto- and genotoxic effects resulted from short-term exposure of human lung epithelial cells to pristine (as manufactured) and user-tailored SWCNTs, as a function of their physicochemical properties. (cdc.gov)

[ 5 ] ASL depletion resulted in reduced mucus clearance and histologic signs of chronic airway disease, including mucous obstruction, goblet cell hyperplasia, and chronic inflammatory cell infiltration. (medscape.com)
It has been previously linked with bronchiolitis obliterans, a debilitating lung disease that was dubbed "popcorn lung" because it first appeared in workers who inhaled artificial butter flavor in microwave popcorn processing facilities. (newswise.com)
We have developed sophisticated preclinical models that mimic the metastatic behaviour of the human disease. (edu.au)
Fever, tachycardia, and elevated white blood cell count have been reported in the absence of an identifiable infectious disease. (cdc.gov)

Prolonged EVLP using OCS lung: cellular and acellular perfusates. (nature.com)

IPF mucus cells expressed MUC5B but low levels of MUC5AC and MUC2, a profile typical of submucosal glands. (bmj.com)

Our data reveal that influenza viruses cause an overall down-regulation of the host p53 pathway and highlight this pathway and p53 protein itself as important viral targets in the altering of apoptotic processes and in cell-cycle regulation. (nih.gov)
The scientists write that this level of infectivity "greatly enhances the opportunity for virus adaptation in humans and raises concerns for the possible generation of pandemic viruses. (medicalnewstoday.com)
The work comes as a salutary reminder that we are constantly at risk of new emergence of zoonotic pathogens and that farmed animals, with which humans have greater contact than with wildlife, may act as the source for important pandemic viruses. (medicalnewstoday.com)

Additionally, lung organoids also express angiotensin-converting enzyme 2 (ACE2), the receptor for the novel SARS-CoV-2 virus that causes COVID-19, along with TMPRSS2, the serine protease that enhances SARS-CoV-2 viral entry. (sigmaaldrich.com)
SARS-CoV-2 cell entry depends on ACE2 and TMPRSS2 and is blocked by a clinically proven protease inhibitor. (invivogen.com)
Each cell line was incubated with Anti-ACE2-PE antibody for 1 hour at 4°C. Cell surface staining was analyzed by flow cytometry. (invivogen.com)

The interaction between epithelial and stromal cells through soluble factors such as cytokines plays an important role in carcinogenesis. (psu.edu)

Human NLRP1 is a sensor of pathogenic coronavirus 3CL proteases in lung epithelial cells. (invivogen.com)

Satellite cells are essential for skeletal muscle regeneration:the cell on the edge returns centre stage. (scielo.br)

STARD14 was negatively associated with the infiltration of CD8+T cells, while positively with CCL28 and immune checkpoints, including CTLA4 as well as PD-L2. (medsci.org)
IgM is the first class of immunoglobulin that is made by immune cells when stimulated by antigen. (cdc.gov)

Public health officials and others concerned with appropriate actions to take at hazardous waste sites may want information on levels of exposure associated with more subtle effects in humans or animals (LOAELs) or exposure levels below which no adverse effects (NOAELs) have been observed. (cdc.gov)
Studies of human exposure to petroleum (crude oil and fuel) often involve monitoring volatile monoaromatic compounds because of their toxicity and prevalence. (cdc.gov)

OCT4 as a Target of miR-34a Stimulates p63 but Inhibits p53 to Promote Human Cell Transformation. (nih.gov)

Anatomy37

Organisms14

Diseases21

Chemicals and Drugs101

Analytical, Diagnostic and Therapeutic Techniques and Equipment28

Psychiatry and Psychology1

Phenomena and Processes51

Disciplines and Occupations1

Technology, Industry, Agriculture2

Information Science3

Alveolar18

Bronchial7

A54911

Fibrosis17

Differentiation14

Mesenchymal4

Proliferation4

Distal lung2

Fibroblasts1

Tissue12

Epithelium1

Progenitor Cells1

Respiratory5

Tissues9

Extracellular matrix1

Embryonic2

Primary3

Biology5

Vivo3

Genes1

Airways5

Innate4

Organoid1

Tumors2

Induced plur1

Mice1

Integrity4

Gene6

Squamous cell carc2

Populations2

MRNA2

Infection4

Activation3

20191

Infect4

Transplantation3

Cancer19

Abstract1

Diseases4

Lesions1

Pulmonary hypertension1

Hypoxia2

Inhibition1

Synergistic1

Disease4

Cellular1

MUC5AC1

Viruses3

ACE23

Interaction1

Pathogenic1

Regeneration1

Immune2

Exposure2

Stimulates1