Immunohistochemistry-Paraffin: HLA DRB1 Antibody (LN-3 + HLA-DRB/1067) [NBP2-45316] - Human Tonsil stained with HLA-DRB Monoclonal Antibody (LN-3 + HLA-DRB/1067). (novusbio.com)
This monoclonal antibody reacts with the beta-chain of HLA-DRB1 antigen, a member of MHC class II molecules. (novusbio.com)
The following product was used in this experiment: HLA-DR Monoclonal Antibody (LN3), Super Bright™ 436, eBioscience™ from Thermo Fisher Scientific, catalog # 62-9956-42, RRID AB_2744822. (thermofisher.com)
Haplotypes3
The genetic susceptibility to celiac disease is conferred by well-identified haplotypes in the human leukocyte antigen (HLA) class II region (ie, DR3 or DR5/DR7 or HLA DR4). (medscape.com)
Gluten is the single major environmental factor that triggers celiac disease, which has a narrow and highly specific association with class II haplotypes of HLA DQ2 (haplotypes DR-17 or DR5/7) and, to a lesser extent, DQ8 (haplotype DR-4). (medscape.com)
HLA- DQ2 and HLA-DQ8 testing is valuable because CD is unlikely if both haplotypes are negative. (vdocuments.net)
Alleles2
PsA has been associated with human leucocyte antigen (HLA) class 1 alleles as opposed to the class 2 alleles associated with RA. (bmj.com)
In the case of systemic lupus erythematosus (SLE) and related illnesses, many of the HLA class II alleles are associated with the presence of specific autoantibodies or clinical phenotypes. (musculoskeletalkey.com)
Antibody5
This antibody does not cross-react with HLA-DR2 and HLA-DR7. (bdbiosciences.com)
Western Blot: HLA DRB1 Antibody (LN-3 + HLA-DRB/1067) [NBP2-45316] - Western Blot Analysis of Ramos cell lysate using HLA DRB1 Antibody (LN-3 + HLA-DRB/1067). (novusbio.com)
Flow Cytometry: HLA DRB1 Antibody (LN-3 + HLA-DRB/1067) [NBP2-45316] - Flow Cytometric Analysis of Human Raji cells using HLA DRB1 Antibody (LN-3 + HLA-DRB/1067). (novusbio.com)
Applications Tested: This LN3 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. (thermofisher.com)
Interestingly, the recent data in RA indicate that the major HLA-DR associations are with anti-CCP antibody positive disease, suggesting that control of autoantibody responses may be a primary mechanism underlying these associations in RA as well. (musculoskeletalkey.com)
Molecules9
In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells. (novusbio.com)
HLA-DR, like other MHC class II molecules, is a transmembrane glycoprotein composed of a 36 kDa alpha chain (DRA) and 27 kDa beta chain (DRB). (thermofisher.com)
The human MHC class II molecules bind intracellularly processed peptides, present them to T-helper cells, and have a critical role in the initiation of the immune response. (thermofisher.com)
The HLA molecules and their counterparts in rodents were subsequently shown to be directly responsible for immune response differences between individuals and for determining the likelihood of graft rejection. (musculoskeletalkey.com)
In each individual, T cells are generally restricted to recognize antigens presented by the person's own HLA molecules. (musculoskeletalkey.com)
The allelic variations among different HLA molecules are a major factor accounting for differences in the types of antigenic peptides to which an individual responds or in the types of T cells that are used in an immune response. (musculoskeletalkey.com)
HLA class I molecules consist of a 45-kD α chain encoded within the MHC that is noncovalently associated with the 12-kD β 2 -microglobulin chain (encoded on chromosome 15). (musculoskeletalkey.com)
HLA class II molecules consist of noncovalently associated α (32 kD) and β (28 kD) chains, both of which are encoded within the MHC. (musculoskeletalkey.com)
HLA class I and class II molecules are cell surface glycoproteins, anchored to the membrane by hydrophobic transmembrane segments. (musculoskeletalkey.com)
Genotype1
The analysis of genetic factors other than CFTR may help our understanding of genotype-phenotype relationships in CF. As human leukocyte antigen (HLA) class II polymorphism has been associated with a number of diseases including autoimmune and inflammatory diseases, asthma, and allergy, we investigated the possibility that HLA polymorphism contributes to CF-associated pulmonary inflammation. (nih.gov)
Cross-react1
It does not cross react with HLA-DP and HLA-DQ. (novusbio.com)
Complement1
This interaction results in complement activation with glomerular infiltration of polymorphonuclear leukocytes (PMNs) and monocytes. (medscape.com)
Antibodies1
Following this model, the human immune system is divided into two major classes: Cellular Immunity, located in the mucous membranes of the gastrointestinal and respiratory tracts and their respective lymph nodes (outer defenses), and Humoral Immunity, with production of antigen-specific antibodies by plasma cells in the bone marrow (inner defenses). (medicalveritas.org)
HLA-DR is a heterodimeric transmembrane protein composed of alpha and beta subunits and plays an important role in the presentation of peptides to CD4+ T lymphocytes. (thermofisher.com)
HLA-DQ antigens exits as heterodimers comprised of polymorphic transmembrane HLA-DQ alpha and beta glycoproteins. (bdbiosciences.com)
Peptide2
Modified peptides showed enhanced binding to HLA-A2 compared with the native sequences and immunization of HLA-A2 transgenic mice generated high avidity modification specific CD8 responses that killed peptide expressing target cells. (bmj.com)
The polymorphisms associated with the "shared epitope" are located on the α-helical rim (DRB1 chain) of the peptide-binding cleft, where they may interact with either the bound peptide antigen or the T cell receptor. (musculoskeletalkey.com)
Peptides3
Recent observations raise the hypothesis that not only the drug/chemical, but also parts of the haptenated protein or peptides may constitute the important structural determinants for antigen recognition by the TCR. (frontiersin.org)
Methods Homocitrullinated peptides were identified and assessed in vitro for HLA-A2 binding and in vivo in human leukocyte antigen (HLA) transgenic mouse models for immunogenicity. (bmj.com)
Human tumor samples were analyzed by targeted mass spectrometry for presence of homocitrullinated peptides. (bmj.com)
Recognize2
Activated B cells and memory T cells can recognize specific antigens on pathogens. (amboss.com)
Background Post-translational modification of proteins has the potential to alter the ability of T cells to recognize major histocompatibility complex (MHC) class -I and class-II restricted antigens, thereby resulting in altered immune responses. (bmj.com)
Monocytes1
Description: The LN3 mAb reacts with the human major histocompatibility complex (MHC) class II, HLA-DR. HLA-DR is expressed on the surface of human antigen presenting cells (APC) including B cells, monocytes, macrophages, DCs, and activated T cells. (thermofisher.com)
Heterodimeric1
HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36kDa alpha (heavy) chain and a 28kDa beta (light) chain. (novusbio.com)
The MHC, one of the most gene-rich regions in the human genome, has identified more than 200 genes. (musculoskeletalkey.com)
Cells5
During a streptococcal infection, mature antigen-presenting cells such as B cells present the bacterial antigen to CD4+T cells which differentiate into helper T2 cells. (wikipedia.org)
HLA-DR antigens also occur on a variety of epithelial cells and their corresponding neoplastic counterparts. (novusbio.com)
During thymic selection, T cells that have not yet encountered their cognate antigen are considered naive T cells. (frontiersin.org)
Due to the artificial nature of drug/chemical-T-cell epitopes, it is not clear whether thymic selection of drug/chemical-specific T cells is a common phenomenon or remains limited to few donors or simply does not exist, suggesting T-cell receptor (TCR) cross-reactivity with other antigens. (frontiersin.org)
Nevertheless, there are only an estimated 10 12 T cells in the human body ( 26 ). (frontiersin.org)
Tumor2
Loss of HLA-DR expression is related to tumor microenvironment and predicts adverse outcome in diffuse large B-cell lymphoma. (novusbio.com)
In addition, the homocitrullinated aldolase epitope was also detected in human tumor samples. (bmj.com)
Peripheral1
In AS patients, the absence of HLA-B27 seems to be related to an increase in the probability of starting the disease with cervical pain and peripheral manifestations. (bvsalud.org)
Major1
Three loci, DR, DQ and DP, encode the major expressed products of the human class II region. (thermofisher.com)
Positive1
In PsA, the onset of arthritis and psoriasis was more prevalent in HLA-B27-negative patients, while initiation with axial manifestations was more predominant in HLA-B27-positive patients. (bvsalud.org)
Specific1
The innate immune system develops in utero and, unlike the adaptive (acquired) immune system , does not require imprinting or adaptation to specific antigens nor does it provide permanent pathogen -specific immunity . (amboss.com)
Diseases1
Many common human diseases such as asthma, cancer, and cardiovascular disease are complex in nature, as they are influenced variably by environmental, physiologic, and genetic factors. (cdc.gov)
System1
Although the human immune system is of almost inconceivable complexity in its detailed functions, the basic principles are quite simple, which might be compared with a medieval castle with an outer mote, an outer wall with parapets, and an inner defense wall, all of which serve to protect the king (brain and nervous system) and queen (genetic system). (medicalveritas.org)
Mature2
Any of the immature or mature forms of a granular leukocyte that in its mature form has an irregularly shaped, pale-staining nucleus that is partially constricted into two lobes, and with cytoplasm that contains coarse, bluish-black granules of variable size. (immport.org)
Any of the immature or mature forms of a granular leukocyte with a nucleus that usually has two lobes connected by one or more slender threads of chromatin, and cytoplasm containing coarse, round granules that are uniform in size and which can be stained by the dye eosin. (immport.org)
Patients1
OBJECTIVE: To describe and analyse the initial symptoms attributable to patients with spondyloarthritis (SpA) and their association with HLA-B27 status. (bvsalud.org)
Presence1
CONCLUSION: In this SpA population, MMs were the most prevalent initial symptoms, with differences across diagnoses and depending on the presence of the HLA-B27 antigen. (bvsalud.org)
Disease1
During this time, the perception of CD has changed from a rather uncommon enteropathy to a common multi- organ disease with a strong genetic predisposition that is associated mainly with human leukocyte antigen (HLA)-DQ2 and HLA-DQ8. (vdocuments.net)
Status1
Differences in the first sign(s) or symptom(s) were compared across diagnoses and between HLA-B27 status. (bvsalud.org)