Human leukemia cell sensitive activity in l1210 leukemia cells. Medical search. Frequent questions

We have observed that DAC reduced the in vitro clonogenic potential in a dose-dependent manner of both human and murine leukemia and tumor cell lines. (biomedcentral.com)

In this study, we investigated the effects of Accepted 14 September 2009 nine naturally occurring compounds isolated from Citrus jambhiri Lush and Citrus pyriformis Hassk Available online 24 September 2009 (Rutaceae) for their potential to modulate the activity of P-gp in the multidrug-resistant human leukaemiacell line CEM/ADR5000. (gotomydoctor.com)

Peripheral blood mononuclear cells of healthy donors and post-transplant lymphoma patients were stimulated with or without lunasin in the presence of IL-12 or IL-2. (weeksmd.com)
The combination of lunasin and cytokines (IL-12 plus IL-2) was capable of restoring IFNÎ³ production by NK cells from post-transplant lymphoma patients. (weeksmd.com)

NF-jB knockdown did not induce death under normal growth conditions, but did reduce TNFa-dependent cell survival. (lsd1receptor.com)
Around the same time, research began to show that the regulation of iron is a central function of the immune system, and that this seems to have evolved because iron is a basic requirement for the survival and growth of cells of all types, including bacteria, parasites, and cancer. (raypeatforum.com)

We measured its inhibitory activity on 17ß-HSD3 using microsomal fraction of rat testes as well as transfected LNCaP[17ß-HSD3] cells. (bvsalud.org)
The addition of a picolyl moiety at C-16 of RM-532-105 steroid core improves the 17ß-HSD3 inhibitory activity in the microsomal fraction of rat testes, but not in whole LNCaP[17ß-HSD3] cells. (bvsalud.org)
Because the inhibitory activity data against 17ß-HSD3 suggested that both steroid derivatives are non-competitive inhibitors, we performed docking and molecular dynamics simulations using a homology model of this membrane-associated enzyme. (bvsalud.org)

This enzyme has a central role in establishing and maintaining both cytosolic and mitochondrial folylpolyglutamate concentrations and, therefore, is essential for folate homeostasis and the survival of proliferating cells. (wikipedia.org)
Most cancer cells undergo metabolic reprogramming that support their survival and proliferation. (degruyter.com)
This indicates the intricate control of metabolism and redox balance for survival of cancer cells that can be targeted by therapeutic agents. (degruyter.com)
The in vivo antineoplastic activity of DAC administered as an i.v. infusion was evaluated in mice with murine L1210 leukemia by measurement of survival time, and in mice bearing murine EMT6 mammary tumor by excision of tumor after chemotherapy for an in vitro clonogenic assay. (biomedcentral.com)

an enzyme linked immunosorbent assay (elisa) system to study specific anti-herpes simplex virus (hsv) antibody production in vitro by human peripheral blood mononuclear cells (pbmc) has been developed. (liverpool.ac.uk)

Clonogenic assays were performed on leukemic and tumor cell lines to evaluate the in vitro antineoplastic activity of DAC. (biomedcentral.com)
Increasing the DAC concentration and duration of exposure produced a greater loss of clonogenicity for both human leukemic and tumor cell lines. (biomedcentral.com)
The reactivation of the TSGs ( p57KIP2 in HL-60 leukemic cells and p16CDKN2A in Calu-6 lung carcinoma cells) and the inhibition of global DNA methylation in HL-60 leukemic cells increased with DAC concentration. (biomedcentral.com)

Functional loss of the reduced folate carrier enhances the antitumor activities of novel antifolates with selective uptake by the proton-coupled folate transporter. (wayne.edu)
The role of autophagy in the death of L1210 leukemia cells initiated by the new antitumor agents, XK469 and SH80, Mol Cancer Ther 2007 Jan;6(1):370-9. (wayne.edu)

Folylpolyglutamate synthase, mitochondrial is an enzyme that in humans is encoded by the FPGS gene. (wikipedia.org)

NK activation was evaluated, and its tumoricidal activity was assessed using in vitro and in vivo tumor models. (weeksmd.com)
In addition, NK cells stimulated with lunasin plus cytokines displayed higher tumoricidal activity than those stimulated with cytokines alone using in vitro and in vivo tumor models. (weeksmd.com)
in vitro and in vivo antiviral activity of 2'-fluorinated arabinosides of 5-(2-haloalkyl)uracil. (liverpool.ac.uk)
5-(2-fluoroethyl)-2'-deoxyuridine (fedu), its 2'-fluoroarabinofuranosyl analog (fefau) and the 2'-fluoroarabinofuranosyl analog (cefau) of the potent anti-herpesvirus compound 5-(2-chloroethyl)-2'-deoxyuridine (cedu) were evaluated for activity against herpes simplex virus type 1 (hsv-1) and hsv-2 in vitro and in vivo. (liverpool.ac.uk)
limiting dilution analysis of specific in vitro anti-herpes simplex virus antibody production by human lymphocytes. (liverpool.ac.uk)
trifluorothymidine (tft) is known to be concentrated in herpes simplex virus (hsv) infected cells in vitro in the form of phosphorylated derivatives. (liverpool.ac.uk)
In order to clarify the dose-schedule of DAC as a guide to clinical trials, we have evaluated the antineoplastic activity of DAC both in vitro and in animal models. (biomedcentral.com)
The in vitro antineoplastic action of DAC correlated well with its inhibition of DNA methylation and reactivation of TSG in human neoplastic cells. (biomedcentral.com)
We also observed a correlation between in vitro and in vivo concentrations needed to produce optimal responses in mice with L1210 leukemia or with EMT6 tumor. (biomedcentral.com)
an ATP-binding ), coumarins () and terpenoids cassette (ABC) transporter (150-170 kDa), which pumps out lipo- have been reported as philic agents from cells that have entered them by free diffusion agents for overcoming multidrug resistance, and could be used aloneat very low concentrations or in combination to reverse multidrugresistance in vitro. (gotomydoctor.com)

consequently, genes encoding some of these proteins are being used to design constructs for the inhibition of multiplying cancer cells. (weeksmd.com)
Furthermore, in tumour necrosis factor alpha (TNFa)-stimulated Ewing's sarcoma (ES) cells, NF-jB is reported to prevent activation of an apoptotic cascade through inhibition of c-Jun N-terminal kinase (JNK) [23] and upregulation of anti-oxidant enzymes [24]. (lsd1receptor.com)

upon elevation of Ca2+ and IP4 concentrations, the PH domain binds to IP4 thereby stimulating catalytic activity, while at the same time the C2a domain takes over the role of membrane tether. (sdbonline.org)
Selected flavonoids were tested for their ability to inhibit the catalytic activity of DNA topoisomerase (topo) I and II. (ucy.ac.cy)

The Gap1 catalytic domain alone is insufficient for in vivo activity of Gap1, indicating a requirement for the additional domains. (sdbonline.org)
An inositol-1,3,4,5-tetrakisphosphate (IP4)-sensitive extended PH domain is essential for Gap1 activity, while Ca2+-sensitive C2 domains and a glutamine-rich region contribute equally to full activity in vivo. (sdbonline.org)
Resolution of these problems has been approached in Drosophila by carrying out an in vivo functional analysis of Drosophila Gap1 in the context of cone cell determination in the developing Drosophila eye. (sdbonline.org)

Magnetic resonance imaging measurements of the response of murine and human tumors to the vascular-targeting agent ZD6126. (wayne.edu)
In mice with L1210 leukemia and in mice bearing EMT6 tumors, the antineoplastic action of DAC also increased with the dose. (biomedcentral.com)

Consequently, ROS-modulation has emerged as an anticancer strategy with synthesis of various ROS-inducing or responsive agents that target cancer cells. (degruyter.com)

We have shown that intensification of the DAC dose markedly increased its antineoplastic activity in mouse models of cancer. (biomedcentral.com)

it was reported that glycoprotein c of hsv-1 (gc-1) mediates the binding of c3b-coated erythrocytes to infected cells and has regulatory (decay-accelerating) activity for the alternative pathway c3 convertase of human complement. (liverpool.ac.uk)

To introduce altered Drosophila Gap1 transgenes into flies, the sevenless ( sev ) enhancer/promoter cassette, an eye-specific promoter, was used to drive expression in cone cell precursors at appropriate levels. (sdbonline.org)
A neoplasm characterized by abnormalities of the lymphoid cell precursors leading to excessive lymphoblasts in the marrow and other organs. (lookformedical.com)

One such peptide, Lunasin , has been shown to selectively act on newly transformed cells while having no cytotoxic effect on non-tumorigenic or established cancer cell lines. (weeksmd.com)
We then assessed its metabolic stability as well as its cytotoxic effect against a panel of cancer cell lines. (bvsalud.org)
Interestingly, this structural modification enhances 3-fold the metabolic stability in conjunction with a significant cytotoxic effect against pancreatic, ovarian, breast, lung, and prostate cancer cells. (bvsalud.org)

Of note, an increased carbohydrate uptake and/or induction of death receptors of cancer cells was exploited to develop glycoconjugates that potentially induce cellular stress, ROS and apoptosis. (degruyter.com)
Cancer cells also enhance glucose-uptake to fuel these enhanced metabolic activities [ 2 ]. (degruyter.com)

The exact mechanism of fen-retinide-induced cell death is not fully understood, although it is reported to induce apoptosis indepen-dently of retinoic acid receptor expression [6-9], lacking the carboxyl group required for binding to these receptors [10]. (lsd1receptor.com)
More recently, fenretinide-induced apoptosis has been reported to be dependent on phosphorylation of nuclear factor-kappa B (NF-jB) in SH-SY5Y neuroblastoma cells [13]. (lsd1receptor.com)
The NF-jB family of transcription factors are involved in the regulation of several physiological processes such as inflammation, cell cycle regulation and apoptosis. (lsd1receptor.com)
Consistent with this hypothesis, NF-jB is constitutively acti-vated in a wide variety of tumours permitting cells to evade apoptosis [16-19,21,22]. (lsd1receptor.com)

2007). Encapsulation in liposomal nanoparticles enhances the immunostimulatory, adjuvant and anti-tumor activity of subcutaneously administered CpG ODN. (ubc.ca)

This mini review highlights the development of compounds that target cancer cells by taking advantage of redox or metabolic alteration in cancer cells. (degruyter.com)
High abundance of glucose in the cytoplasm of a cancer cell also increases flux into other metabolic pathways such as hexosamine biosynthetic pathway (HBP) [ 7 ]. (degruyter.com)

Numerous vectors have been engineered for the sole purpose of killing cancer cells, and some have successfully suppressed malignant tumours. (weeksmd.com)

Eukaryotic expression vectors containing genes encoding plant proteins for killing of cancer cells. (weeksmd.com)
2005). Investivgation of factors responsible for cell line cytoplasmic expression differences. (ubc.ca)

Therefore, the quantities of these compounds in human diet can be quite significant. (encyclopedia.pub)
Treatment of drug-resistant Caco-2 cells with the most active C. jambhiri and C. pyriformis compounds increased their sensitivity to doxorubicin and completely reversed doxorubicin resistance, which agrees with a decreased P- gp activity. (gotomydoctor.com)

5-ethyl-2'-deoxyuridine (edu) is a potent and selective inhibitor of the replication of herpes simplex virus type 1 (hsv-1) and 2 (hsv-2), which is currently being pursued for the topical treatment of hsv-1 and hsv-2 infections in humans. (liverpool.ac.uk)

Fenretinide (N-(4-hydroxyphenyl)retinamide) is a synthetic vitamin A analogue with chemopreven-tive and therapeutic activity against a wide range of cancers [1]. (lsd1receptor.com)
The pioneer researcher in the role of iron in immunity believed that an excess of dietary iron contributed to the development of leukemia and lymphatic cancers. (raypeatforum.com)

In most cell types, NF-jB is sequestered in the cytoplasm by association with IjBa [20]. (lsd1receptor.com)

The underlying mechanism responsible for the effects of lunasin on NK cells is likely due to epigenetic modulation on target gene loci. (weeksmd.com)
Gene therapy has attracted attention for its potential to specifically and efficiently target cancer cells with minimal toxicity to normal cells. (weeksmd.com)

Cancer cells often maintain high basal level of ROS and are vulnerable to any further increase in ROS levels beyond a certain protective threshold. (degruyter.com)
This is because cancer cells maintain high basal level of ROS, thus they are vulnerable to any further increase in ROS (Fig. 1 ). (degruyter.com)
Basal levels of phosphorylated NF-jB were low in all ESFT cells. (lsd1receptor.com)

BAY 11-7082-induced cell death through an NF-jB-independent mechanism and enhanced cell death when combined with fenretinide. (lsd1receptor.com)
of cell death [7]. (lsd1receptor.com)
In this study we have investigated the role of NF-jB in fenretinide-induced cell death and the importance of NF-jB in ESFT. (lsd1receptor.com)

BAY 11-7082 decreased cell viability, which was accompanied by caspase-3 cleavage. (lsd1receptor.com)

Bilirubin is a chemical that is produced when old red blood cells break down. (webmd.com)

Redirection of glutamine to cellular energetics destabilizes glutathione homeostasis in glioblastoma cells and other cancer cells [ 9 ]. (degruyter.com)

Adding lunasin to IL-12- or IL-2-stimulated NK cells demonstrated synergistic effects in the induction of IFNG and GZMB involved in cytotoxicity. (weeksmd.com)

Others may act through activation of T-CELLS or by inhibiting the activation of HELPER CELLS. (lookformedical.com)

ROS-modulation in normal vs. cancer cell. (degruyter.com)

using [4-14c]edu as the radiolabeled analogue of edu, it was ascertained that, at antivirally active doses, edu is phosphorylated to a much greater extent by hsv-infected vero cells than by mock-infected cells. (liverpool.ac.uk)

The drug is used in the therapy of acute leukemia. (lookformedical.com)

It interferes with nucleic acid synthesis by inhibiting purine metabolism and is used, usually in combination with other drugs, in the treatment of or in remission maintenance programs for leukemia. (lookformedical.com)
It has been used similarly to MERCAPTOPURINE in the treatment of leukemia. (lookformedical.com)

They frequently play an anti-apopto-tic role [14-16], but may also be pro-apoptotic in some cell types and conditions [16-19]. (lsd1receptor.com)

Tumor cell resistance to anthracyclines and epipodophyllotoxins can be due to reduced drug accumulation and/or alterations in the activity of topoisomerase II (TOPO II). (ucy.ac.cy)
2008) The effect of circulation lifetime and drug-to-lipid ratio of intravenously administered lipid nanoparticles on the biodistribution and immunostimulatory activity of encapsulated CpG-ODN. (ubc.ca)

Anatomy25

Organisms12

Diseases42

Chemicals and Drugs110

Analytical, Diagnostic and Therapeutic Techniques and Equipment20

Phenomena and Processes49

Information Science3

Murine leukemia1

Multidrug resistant1

Lymphoma2

Growth2

Inhibitory Activity3

Survival4

Mononuclear cells1

Leukemic3

Antitumor2

Enzyme1

Vitro10

Inhibition2

Catalytic activity2

Vivo3

Tumors2

Anticancer1

Antineoplastic1

Mediates1

Precursors2

Cytotoxic effect3

Uptake2

Apoptosis4

Enhances1

Metabolic2

Tumours1

Expression2

Compounds2

Potent1

Cancers2

Cytoplasm1

Gene2

Basal3

Death3

Cleavage1

Chemical1

Homeostasis1

Induction1

Activation1

Normal1

Analogue1

Therapy1

Treatment2

Role1

Drug2