• Here we show that methanolic extracts of Sapota fruit (MESF) induces cytotoxicity in a dose-dependent manner in cancer cell lines. (nature.com)
  • In a preliminary study it has been shown that phenolic antioxidants such as methyl 4- O -galloylchlorogenate and 4- O -galloylchlorogenic acid derived from Sapota fruits can induce cytotoxicity in colon cancer cells 23 . (nature.com)
  • Expanded natural killer cells killed both allogeneic and autologous primary myeloma cells avidly via a perforin-mediated mechanism in which the activating receptor NKG2D, natural cytotoxicity receptors, and DNAX-accessory molecule-1 played a central role. (haematologica.org)
  • Chaetominine has cytotoxicity against human leukemia K562 and colon cancer SW1116 cell lines. (abmole.com)
  • Further, cytotoxicity towards different tumor cell lines was assessed via LDH assays and flow cytometry-based degranulation assays. (infinite-science.de)
  • NK cells showed cytotoxicity towards the tumor cell lines K562 (leukemia), PaCa5061 (pancreatic cancer), and SKOV3 (ovarian cancer). (infinite-science.de)
  • In cisplatin-resistant cell lines, BT potentiated cisplatin-induced cytotoxicity at most drug ratios via enhanced ROS generation and modulation of key regulators of apoptosis. (biomedcentral.com)
  • The evaluated functional avidity of these TCRs positively correlated with cell proliferation, cytokine production, and WT1-specific cytotoxicity of the TCR-transduced CD8 + T cells in response to WT1 antigen. (oncotarget.com)
  • In this study, anticancer properties of heterocyclic salicylaldimines and their Cu(II) complexes on K562 human chronic myelogenous leukemia cell line were investigated. (ijpsonline.com)
  • Interestingly, AVM appeared to be more sensitive to imatinib‑resistant (T315I, Y253H, and E279K) than wild‑type BCR/ABL cells, indicating its potential to overcome imatinib‑resistant severe issues in CML. (spandidos-publications.com)
  • Many factors and components play a role in the cell cycle of K562 cells in terms of growth, cell differentiation, and apoptosis. (wikipedia.org)
  • The growth of these leukemic cells are controlled by either initiating cell differentiation or apoptosis to occur. (wikipedia.org)
  • Cell differentiation is induced by the deacetylase activity in these "undifferentiated progenitor cells," which alters the phenotype and morphology of the K562 cells. (wikipedia.org)
  • This gene targets the cyclin-dependent kinase inhibitor, p21, and causes cell differentiation, cell cycle arrest in G1, and ultimately apoptosis. (wikipedia.org)
  • Other methods being focused on in the regulation of K562 cells include therapeutic methods like polyphyllin D, which caused differentiation from the progenitor state to occur, and for apoptosis to begin. (wikipedia.org)
  • In this proposal, we seek to evaluate the integration of K562/GM-CSF vaccination with systemic therapies for MDS that alter host immunity and/or hematopoietic cellular differentiation. (acgtfoundation.org)
  • Inevitably, there are fundamental and applied questions, such as those relating to transcriptional control of stem cell differentiation, intrinsic noise in gene expression, and the origins of disease, that may only be addressed at the single cell level. (justia.com)
  • For erythroid differentiation single-cell suspensions were immunostained with anti-TER119-PE and anti-CD71-FITC (BD Italia Milan Italy) and analyzed by flow cytometry. (bioerc-iend.org)
  • To evaluate the leukemogenic role of RUNX1-MT in ASXL1 -mutated cells, we co-expressed RUNX1 -MT (R135T) and ASXL1 -MT (R693X) in different cell lines and performed immunoblot, co-immunoprecipitation, gene expression microarray, quantitative RT-PCR, cell proliferation, differentiation, and clonogenic assays for in vitro functional analyses. (biomedcentral.com)
  • The expression of RUNX1 mutant in ASXL1 -mutated myeloid cells augmented proliferation, blocked differentiation, and increased self-renewal activity. (biomedcentral.com)
  • Cancer is the uncontrolled growth of cells in the body, which is caused by the stepwise accumulation of mutations that affect cell growth control, differentiation and survival. (nutraculture.com)
  • In this study, we identified miR-199b-5p as a key regulator of human erythropoiesis, and its expression was up-regulated during the erythroid differentiation of K562 cells. (molcells.org)
  • The up-regulation of miR-199b-5p during erythroid differentiation was dependent on the binding of GATA-1 and NF-E2 to its gene locus, which activated its transcription and maintained its high expression level in mature erythroid cells. (molcells.org)
  • Leukemia is a blood cancer that develops in the hematopoietic system and presents in the bone marrow with or without peripheral blood cell involvement[ 1 , 2 ]. (ijpsonline.com)
  • The myelodysplastic syndromes (MDS) are a group of clonal neoplastic hematologic disorders characterized by varying degrees of bone marrow failure, abnormal hematopoiesis, and proliferation of myeloid blast cells. (acgtfoundation.org)
  • Chronic myeloid leukemia (CML) is a malignancy of the blood and bone marrow that affects children and adults. (spandidos-publications.com)
  • 17 Heme content was measured as described elsewhere.18 Erythropoiesis To obtain burst-forming units-erythroid (BFU-E) and colony-forming units-erythroid (CFU-E) 3 cells Linoleylethanolamide from fetal liver or adult bone marrow were cultured in MethoCult M3334 (Stemcell Technologies Vancouver Canada). (bioerc-iend.org)
  • Mice transduced with both ASXL1 and RUNX1 mutations enhanced inhibitor of DNA binding 1 (ID1) expression in the spleen, liver, and bone marrow cells. (biomedcentral.com)
  • Human and mouse T cells treated with ITFG1 in vitro secreted the cytokines IFN-g, TNF-a and IL-10, while in vivo ITFG1 was protective in a mouse acute graft-versus-host disease (GVHD) model. (rndsystems.com)
  • Multidrug resistance parameters, tissue infiltration parameters, receptors for colony-stimulating factors (CSFr) and cell cycle parameters were analyzed using flow cytometry in 145, 109 initial and 36 relapsed or refractory, acute nonlymphoblastic leukemia (ANLL) patients to find out clinically more reliable functional parameters. (karger.com)
  • Although the disease can evolve toward acute leukemia, morbidity and mortality most frequently result from a marrow failure syndrome. (acgtfoundation.org)
  • In early phase clinical trials for both acute and chronic myeloid leukemias, we have observed the induction of anti-tumor immunity and associated clinical responses following K562/GM-CSF vaccination (see preliminary data). (acgtfoundation.org)
  • Leukemias were originally termed acute or chronic based on life expectancy but now are classified according to cellular maturity. (curehunter.com)
  • However, Zweidler-McKay and co-senior investigator Elizabeth Shpall, M.D., professor in M. D. Anderson's Department of Stem Cell Transplantation and Cellular Therapy, have found a novel process to increase NK cells in cord blood more than 30-fold, generating more than 150 million NK cells from one cord blood unit while maintaining their activation to find and kill acute leukemia cells. (biologynews.net)
  • When given to mice with aggressive human leukemias, these NK cells reduced the circulating human acute lymphocytic leukemia (ALL) and acute myelogenous leukemia (AML) cells by 60 to 85 percent. (biologynews.net)
  • These results support the evaluation of cord blood-derived NK cells as a potential immuno-therapeutic approach in acute leukemias. (biologynews.net)
  • Many ORs are expressed by tumor cells and tissues, suggesting that they may be associated with cancer progression or may be cancer biomarkers. (bmbreports.org)
  • They are able to fight circulating tumor cells thereby preventing metastases formation which account for approximately 90% of all cancer-deaths. (infinite-science.de)
  • To investigate the possibility of killing tumor cells by the expression of an exogenously introduced toxic gene, we have constructed a novel retroviral vector (LTRNL) which has the polyA signal deleted herpes simplex virus type 1 thymidine kinase (HSV1-tk) gene. (elsevierpure.com)
  • The cells are non-adherent and rounded, are positive for the bcr:abl fusion gene, and bear some proteomic resemblance to both undifferentiated granulocytes and erythrocytes. (wikipedia.org)
  • There are many different cellular components involved in the cycle of apoptosis such as BCR/ABL, Bcl-2, Bax protein, and cytochrome C. The tumor suppressor gene p53 is also important in the cell cycle regulation of K562 cells. (wikipedia.org)
  • We tested whether natural killer cells expanded by co-culture with K562 cells transfected with 41BBL and membrane-bound interleukin-15 could kill myeloma cells with a high-risk gene expression profile in vitro and in a unique model which recapitulates human myeloma. (haematologica.org)
  • For example, single cell analysis allows for the direct measurement of gene expression kinetics, or for the unambiguous identification of co-regulated genes, even in the presence of de-synchronization and heterogeneity that could obscure population-averaged measurements. (justia.com)
  • Thus, many of the basic functionalities for microfluidic single cell gene expression analysis have been demonstrated in isolation, including cell manipulation and trapping (9, 10), RNA purification and cDNA synthesis (11-13), and microfluidic qPCR (14) following off-chip cell isolation cDNA synthesis and preamplification. (justia.com)
  • Our experiments indicate that multiple CAD-associated genetic variants located in cell type-specific enhancers are involved in gene regulation in different cells and tissues. (biomedcentral.com)
  • No single causative variant alone, but rather multiple variants combined in a risk haplotype likely contribute to an altered expression of the PKP2 gene, and possibly nearby genes, in immune and epithelial cells, and predispose GSDs to CAD. (biomedcentral.com)
  • Cell culture and gene silencing The human lymphoblast K562 cell line (ATTC number CCL-243? (bioerc-iend.org)
  • Moreover, the RUNX1 mutant protein was more stable than WT and increased HIF1-α and its target ID1 gene expression in ASXL1 mutant cells. (biomedcentral.com)
  • In the 2D3, the precise TCR functional avidity of transduced TCRs is easily evaluable by the expression of green fluorescent protein (GFP) reporter gene driven by nuclear factor of activated T cells (NFAT) activation via TCR signaling. (oncotarget.com)
  • Four different TCRs of HLA-A * 24:02-restricted Wilms' tumor gene 1 (WT1)-specific CD8 + cytotoxic T lymphocytes (CTLs) were transduced into 2D3 cells and the functional avidities of these four TCRs were evaluated. (oncotarget.com)
  • These preclinical and clinical evidences encourage us to develop T-cell adoptive immunotherapy using genetically engineered T cells that are transduced with a T-cell receptor (TCR) gene specific for TAA. (oncotarget.com)
  • We analyzed differential gene expression in the peripheral white blood cells (WBC) from 7 female patients diagnosed with occupational benzene poisoning and 7 matched controls. (cdc.gov)
  • K562 cells were the first human immortalised myelogenous leukemia cell line to be established. (wikipedia.org)
  • K562 cells are of the erythroleukemia type, and the cell line is derived from a 53-year-old female chronic myelogenous leukemia patient in blast crisis. (wikipedia.org)
  • Western blot analysis of Rab27 in human chronic myelogenous leukemia. (bdbiosciences.com)
  • Three studies have shown that monoclonal antibodies to the DENV fusion loop epitope reliably enhanced Zika virus infection (i.e., antibody-dependent enhancement [ADE]) in Fc receptor-bearing K-562 human myelogenous leukemia cells or the U-937 human monocytic cell line ( 1 - 3 ). (cdc.gov)
  • Moreover, a Topo I-altered cell line (KBSTP2), resistant to SN38, remains sensitive to BN80927, suggesting that a part of the antiproliferative effects of BN80927 are mediated by a Topo I-independent pathway. (aacrjournals.org)
  • This hypothesis is also supported by in vitro data showing an antiproliferative activity of BN80927 on a model of resistance related to the noncycling state of cells (G 0 -G 1 synchronized). (aacrjournals.org)
  • In cell growth assays, BN80927 is a very potent antiproliferative agent as shown by IC 50 values consistently lower than those of SN38 in tumor cell lines as well as in their related drug-resistant lines. (aacrjournals.org)
  • Additionally, we observed increased apoptotic morphological changes in berberine treated group.Mangrove fraction exhibit potent antiproliferative effect on AGS cell line and upon further will going to check invivo experimental animal studies. (pdfplayer.org)
  • Antiproliferative activity of Saracatinib varies between cell lines (IC50 0.2-10 μM). (xcessbio.com)
  • Saracatinib potently inhibits the proliferation of Src3T3 mouse fibroblasts and demonstrates variable antiproliferative activity in a range of human cancer cell lines containing endogenous Src. (xcessbio.com)
  • K562 cells are part of the NCI-60 cancer cell line panel used by the National Cancer Institute. (wikipedia.org)
  • The problem with K562 cells, and many other cancer cell types, is an overabundance of Aurora kinases. (wikipedia.org)
  • Inhibiting these is an important regulation mechanism of cancer, because it prevents cells from progressing into mitosis. (wikipedia.org)
  • When the levels of these components are thrown off, they can either no longer inhibit apoptosis of the cancer cells, a role fulfilled by BCR/ABL, or they cause apoptosis to be induced, in the same vein as Bax and cytochrome C. These components are key in the mitochondria, and due to this, it has been supported that apoptosis uses the mitochondrial apoptosis pathway. (wikipedia.org)
  • The ability to induce these changes in K562 cell cycle and cell cycle regulation provides targets for cancer drugs. (wikipedia.org)
  • Annexin V-propidium iodide double-staining demonstrated that Sapota fruit extracts potentiate apoptosis rather than necrosis in cancer cells. (nature.com)
  • In cancer biology, controlling leukemia remains a big challenge. (ijpsonline.com)
  • Similarly, single cell methods are vital in stem cell research and cancer biology, where isolated populations of primary cells are heterogeneous due to limitations in purification protocols, and it is often a minority cell population that is the most relevant. (justia.com)
  • Researchers from The University of Texas M. D. Anderson Cancer Center have found a therapy that effectively kills human leukemia cells in mice using natural killer (NK) cells from umbilical cord blood. (biologynews.net)
  • Patrick Zweidler-McKay, M.D., Ph.D., assistant professor of pediatrics from the Children's Cancer Hospital at M. D. Anderson, has shown an effective method for expanding the number of NK cells from a single cord blood unit while retaining the cells' anti-leukemia effects, as presented at the American Society of Pediatric Hematology/Oncology annual conference on May 16. (biologynews.net)
  • PI-273 can inhibit breast cancer cell proliferation, block the cell cycle and induce cell apoptosis. (abmole.com)
  • Growth of cancer cells is regulated by the balance between cell proliferation and apoptosis, which is a normal physiological process serving to eliminate unwanted cells and maintain homeostasis in healthy tissue. (nutraculture.com)
  • Nutraculture™ Phycocyanin™ induces apoptosis in the existing and proliferating cancer cells and being a natural antioxidant, it also helps getting rid of any cancer promoting oxidative stress. (nutraculture.com)
  • Phycocyanin lowers the amount of cyclooxygenase-2 which is up regulated in cancer cells. (nutraculture.com)
  • It is established that Phycocyanin induces apoptosis in cancer cells by changing the Bcl-2/Bax ratio (Bcl-2 is an anti apoptotic protein, Bax is a pro-apoptotic protein, the ratio of Bcl-2/Bax represents the degree of apoptosis) and the release of Cyt-c in the cytosol (Lu et. (nutraculture.com)
  • Cell cycle involvement in cancer treatment with carvacrol and thymol. (dieselok.md)
  • doi:10.7287/peerj.9626v0.1/reviews/1, Soković, M., Glamoclija, J., and Ciric, A. Thymol also caused a decrease in cell viability of prostate cancer (PC-3 cell line), and provoked cytotoxic effects (Pathania et al. (dieselok.md)
  • 2002). The Mechanism of Carvacrol-Evoked [Ca2+]i Rises and Non-ca2+-triggered Cell Death in OC2 Human Oral Cancer Cells. (dieselok.md)
  • In the present study, we investigated the anticancer effects of adenine and the underlying mechanism in colon cancer cells. (hindawi.com)
  • Adenine significantly inhibited the viability of colon cancer cells, HT29 and Caco-2 cells, in a dose-dependent manner. (hindawi.com)
  • The data showed that adenine activated AMP-activated protein kinase (AMPK) signaling contributing to autophagic cell death through mTOR in both colon cancer cell lines. (hindawi.com)
  • Our findings suggest that adenine inhibits the growth of colon cancer cells. (hindawi.com)
  • Anticancer activity of adenine in colon cancer cells is attributable to the activation of apoptotic signaling and in turn the AMPK/mTOR pathway. (hindawi.com)
  • Recently, adenine has been explored for its anticancer property in several types of cancer cell lines. (hindawi.com)
  • Adenine has been suggested to induce cell cycle arrest in cancer cells, leading to cell death [ 9 , 10 ]. (hindawi.com)
  • However, the mechanism by which adenine inhibits the proliferation of cancer cells is sketchy. (hindawi.com)
  • It is of interest to explore the inhibitory effect of adenine on the growth of colorectal cancer cells and to determine the underlying mechanism. (hindawi.com)
  • In this study, we investigated the effects of adenine against the proliferation of colon cancer cells. (hindawi.com)
  • We explored the possible mechanisms underlying the anticancer activity of adenine in colorectal cancer cells. (hindawi.com)
  • Thus, NK cells became interesting candidates for cancer immunotherapy and ex vivo manipulation and expansion of highly potent NK cells for adoptive transfer in patients is an aim of paramount importance. (infinite-science.de)
  • Using a novel approach for NK cell expansion, we aimed at providing an extensive characterization of the generated cells to evaluate their potential for cancer immunotherapy. (infinite-science.de)
  • We describe a novel approach for ex vivo NK cell expansion generating a set of highly potent NK cells which represent promising candidates for cancer immunotherapy. (infinite-science.de)
  • Cancer is a common term for a large group of disease which are characterized by uncontrolled cell growth and formation of tumors. (pdfplayer.org)
  • Cancer cells can spread to any part of the body and affecting individuals from different sexes, ages and races(American Cancer Society, 2020). (pdfplayer.org)
  • Low doses of BT and cisplatin enhanced efficiency of cisplatin treatment in all the ovarian cancer cell lines tested. (biomedcentral.com)
  • γδ T cells recruited into the tumor microenvironment can act as effector cells to mediate cancer immune surveillance. (explorationpub.com)
  • This review addressed the current progress in the research on cervical cancer, HPV infection, immune cell interaction, and physiological stress and insomnia with the cervicovaginal microbiome to decipher the disease occurrence and therapeutic management. (bvsalud.org)
  • The anticancer effect of capsaicinoids is mainly mediated through mechanisms involving the interaction of Ca2+ -dependent activation of the MAPK pathway, suppression of NOX-dependent reactive oxygen species generation, and p53-mediated activation of mitochondrial apoptosis in cancer cells. (bvsalud.org)
  • Sub micromolar growth inhibition of five of the human cancer cell lines tested with Saracatinib (tumor types: colon, prostate, lung, and leukemia) is observed with IC50 values of 0.2-0.7 μM. (xcessbio.com)
  • In the microdroplet migration assay, Saracatinib reduces the migration of human lung cancer A549 cells in a concentration-dependent manner (IC50 0.14 μM). (xcessbio.com)
  • Adoptive T-cell therapy with T cells expressing chimeric antigen receptors (CARs) is an active area of cancer research. (ashpublications.org)
  • The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
  • Adoptive T-cell therapy with T cell receptor (TCR) -engineered T cells is an attractive strategy for cancer treatment and the success in this therapy is dependent on the functional avidity of the transduced TCRs against targeted tumor antigens. (oncotarget.com)
  • Adoptive immunotherapy using tumor-associated antigen (TAA)-specific CD8 + cytotoxic T lymphocytes (CTLs) and/or CD4 + helper T (Th) cells can induce the regression of large established tumor in not only mouse models but also cancer patients [ 1 - 3 ]. (oncotarget.com)
  • Western Blot: PARP Antibody (194C1439) - Cleaved [NB100-56599] - Analysis of cleaved PARP in staurosporine-treated Jurkat cells at various time points, antibody at 2 ug/mL. (novusbio.com)
  • Apoptosis induction potential of each compound determined by mitochondrial membrane potential analysis (Rho123), cleaved caspase-3 expression analysis by flow cytometry and immunofluorescent staining and cell morphology analysis by giemza, hematoxylin and eosin and Papanicolaou protocols. (ijpsonline.com)
  • In the cell morphology analyzes, chromatin condensation and marginalization, changes in the cell membrane, ghost cells and apoptotic bodies were observed as evidence of apoptosis formation. (ijpsonline.com)
  • These NK cells demonstrate significant cytotoxic activity against human AML and ALL cell lines and patient leukemia blasts. (biologynews.net)
  • Activated γδ T cells exhibit strong cytotoxic activity and cytokine secretion functions and are effective antitumor lymphocytes with simple and direct recognition modes and rapid responses. (explorationpub.com)
  • Rab27A is expressed in many human tissues and it regulates the secretion of several types of cellular vesicles, such as melanosomes, insulin granules of pancreatic beta cells, and lytic granules of cytotoxic T lymphocytes. (bdbiosciences.com)
  • However, the applicability of such traditional chemotherapeutic approaches is primarily limited due to acquired Multi-Drug Resistance (MDR) in cells, which is acquired through the production of anti-apoptotic cascades and the overexpression of cell membrane-based drug efflux pumps, such as P-glycoprotein, for self-defense. (ijpsonline.com)
  • The nuclei of apoptotic and necrosis cells were observed using fluorescence microscopy [13]. (egfrsignals.com)
  • Adenine induced significant apoptosis in HT29 cells, whereas Caco-2 cells exhibited less apoptotic responses. (hindawi.com)
  • Enhanced apoptosis was correlated with loss of pro-survival factors (XIAP, bcl-2, bcl-xL), expression of pro-apoptotic markers (caspases 3/7, PARP cleavage) and enhanced cell cycle regulators p21 and p27. (biomedcentral.com)
  • We have developed a genetically modified tumor cell vaccine for the treatment of myeloid malignancies. (acgtfoundation.org)
  • K562 cells express many of the antigens shown to be overexpressed in myeloid leukemias and MDS. (acgtfoundation.org)
  • In previous research, the authors demonstrated that the methanol extract of Artemisia vulgaris (AVM) has the ability to inhibit chronic myeloid leukemia (CML) cell proliferation. (spandidos-publications.com)
  • Additional sex combs-like 1 ( ASXL1 ) mutations have been described in all forms of myeloid neoplasms including chronic myelomonocytic leukemia (CMML) and associated with inferior outcomes, yet the molecular pathogenesis of ASXL1 mutations ( ASXL1 -MT) remains poorly understood. (biomedcentral.com)
  • Previously, we observed that transcription factor RUNX1 mutations ( RUNX1 -MT) coexisted with ASXL1 -MT in CMML and at myeloid blast phase of chronic myeloid leukemia. (biomedcentral.com)
  • The contribution of RUNX1 mutations in the pathogenesis of myeloid transformation in ASXL1 -mutated leukemia, however, remains unclear. (biomedcentral.com)
  • Co-expression of two mutant genes increased myeloid stem cells in animal model, suggesting that cooperation of RUNX1 and ASXL1 mutations played a critical role in leukemia transformation. (biomedcentral.com)
  • The present study demonstrated the biological and functional evidence for the critical role of RUNX1 -MT in ASXL1 -mutated leukemia in the pathogenesis of myeloid malignancies. (biomedcentral.com)
  • In the present study, we tested whether CX-5461 could potentiate the effect of imatinib in the human chronic myeloid leukemia cell line K562. (mendeley.com)
  • Src and Abl are protein tyrosine kinases that are overexpressed in chronic myeloid leukemia cells. (xcessbio.com)
  • Preclinical data reported here show that BN80927 retains Topo I poisoning activity in cell-free assay (DNA relaxation) as well as in living cells, in which in vivo complexes of topoisomerase experiments and quantification of DNA-protein-complexes stabilization, have confirmed the higher potency of BN80927 as compared with the Topo I inhibitor SN38. (aacrjournals.org)
  • BN80927 shows high efficiency in vivo in tumor xenograft studies using human androgen-independent prostate tumors PC3 and DU145. (aacrjournals.org)
  • The transferred, expanded natural killer cells proliferated in vivo in an interleukin-2 dose-dependent fashion, persisted up to 4 weeks, were readily detectable in the human bone, inhibited myeloma growth and protected bone from myeloma-induced osteolysis. (haematologica.org)
  • Additionally, IV.3 f(ab')2 fragments have been used to block CD32 in vivo in transgenic mice expressing human CD32. (bioxcell.com)
  • Induction of CYP4F3 by benzene metabolites in human white blood cells in vivo, in human promyelocytic leukemic cell lines, and ex vivo in human blood neutrophils. (cdc.gov)
  • Induction of CYP4F3 by phenol was also observed in differentiated HL-60 cells, in pro-erythroid cell line K562, and ex vivo in human neutrophiles. (cdc.gov)
  • The findings demonstrated, for the first time, that benzene and metabolites induce CYP4F3 in human blood cells both in vivo and in vitro. (cdc.gov)
  • Because dengue and Zika viruses co-circulate in the urban Aedes aegypti mosquito-human cycle, a logical question, as suggested by in vitro data, is whether dengue virus infections result in antibody-dependent enhancement of Zika virus infections. (cdc.gov)
  • in vitro cytotoxic effects of GCV to these cells demonstrated that concentrations of about 2.5 μM for K562/LTRNL and 1.25μM for MEG-01/LTRNL cells resulted in 50% inhibition of cell growth after 72 hr. (elsevierpure.com)
  • 15 We have recently conducted experiments that demonstrated enhanced in vitro survival of human T cells that were transduced with an ErbB2-specific CAR when the first and third ITAMs of the CD3-ζ domain of the CAR were inactivated. (ashpublications.org)
  • Chaetominine reduces MRP1-mediated agent resistance via inhibiting PI3K/Akt/Nrf2 signaling pathway in K562/Adr human leukemia cells. (abmole.com)
  • CD32 enables interaction between Fc γ RII-expressing cells and opsonized antigen or IgG-containing immune complexes. (bioxcell.com)
  • Adoptive T-cell therapy with anti-CD19 chimeric antigen receptor (CAR)-expressing T cells is a new approach for treating advanced B-cell malignancies. (ashpublications.org)
  • We used T cells that were retrovirally transduced with this CAR to treat mice bearing a syngeneic lymphoma that naturally expressed the self-antigen murine CD19. (ashpublications.org)
  • 12 , 13 Clinical trials in which patients with advanced B-cell malignancies receive T cells expressing anti-CD19 CARs are in early stages, and it is not known whether adoptive transfer of T cells targeting this self-antigen will be an effective therapy for B-cell malignancies. (ashpublications.org)
  • Design and Methods OPM2 and high-risk primary myeloma tumors were grown in human fetal bone implanted into non-obese diabetic severe combined immunodeficiency mice with a deficient interleukin-2 receptor gamma chain. (haematologica.org)
  • These mice are devoid of endogenous natural killer and T-cell activity and were used to determine whether adoptively transferred expanded natural killer cells could inhibit myeloma growth and myeloma-associated bone destruction. (haematologica.org)
  • This antibody was developed by immunizing mice with a synthetic peptide containing amino acids near 214/215-cleavage site of human PARP. (novusbio.com)
  • Several histological changes, such as spermatogenic cell degeneration, germinal epithelium detachment, and structural abnormalities in seminiferous tubules, were observed in AlCl3-treated mice. (bvsalud.org)
  • Subcutaneous tumors of MEG-01/LTRNL in KSN nude mice, but not those of uninfected MEG-01 cells, showed durable regressions after exposure of the mice to 40 mg/kg of GCV given subcutaneously once a day for 15 days. (elsevierpure.com)
  • One infusion of anti-CD19-CAR-transduced T cells completely eliminated normal B cells from mice for at least 143 days. (ashpublications.org)
  • The antilymphoma efficacy of anti-CD19-CAR-transduced T cells was critically dependent on irradiation of mice before anti-CD19-CAR-transduced T-cell infusion. (ashpublications.org)
  • This allows CD32 to function in the activation or inhibition of immune responses including degranulation, phagocytosis, ADCC, cytokine release, and B cell proliferation. (bioxcell.com)
  • As the cord blood is expanded to multiply in number, the NK cells are given a cytokine, interleukin-2, and a target cell, K562, which keep the NK cells active throughout the three week expansion. (biologynews.net)
  • First, γδ T cells exposed to complicated cytokine networks are potentially affected by multiple inhibitory mechanisms. (explorationpub.com)
  • Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. (bdbiosciences.com)
  • Cell lysates were prepared by incubating 2 × 106 cells ml−1 in extraction buffer for 15 min on ice. (egfrsignals.com)
  • This product is for research use only and is not approved for use in humans or in clinical diagnosis. (novusbio.com)
  • 2. Conduct a clinical trial in MDS testing K562/GM-CSF vaccination integrated with the systemic agent(s) identified in aim 1 as being most active in combination with GM-CSF tumor vaccines (years 2 and 3). (acgtfoundation.org)
  • After having been first isolated in Africa in 1947, human Zika virus disease remained sparse and mild, with no reports of diverse clinical syndromes associated with infection ( 7 ). (cdc.gov)
  • High-throughput single cell measurement technologies are therefore of interest and have broad applications in clinical and research settings. (justia.com)
  • The GMP manufacturing process allows the use of these cells in clinical trials, i.e. adoptive NK cell transfer in patients. (infinite-science.de)
  • However, the clinical application of tumor-infiltrating γδ T cells has certain limitations. (explorationpub.com)
  • Finally, it retrospectively analyze the activation strategies and clinical relevance of existing γδ T cell adoptive immunotherapies. (explorationpub.com)
  • Most CARs that are being evaluated in current clinical and preclinical studies recognize self-antigens that are expressed by normal tissues as well as malignant cells. (ashpublications.org)
  • Adoptive transfer of expanded natural killer cells inhibited the growth of established OPM2 and high-risk primary myeloma tumors grown in the murine model. (haematologica.org)
  • Moreover, infiltration of solid tumors with NK cells has been shown to correlate with a better prognosis for the patient. (infinite-science.de)
  • Their advantage lies in the ability to perceive tumors with a low mutation load, thus establishing the first line of defense against pathogens. (explorationpub.com)
  • These functions are necessary in cells in order to divide and regenerate tissues, and play a maintenance role in homeostatic functions. (wikipedia.org)
  • The sudden appearance of overt human Zika virus infections that cross the placenta to damage fetal tissues, target sexual organs, and are followed in some instances by Guillain-Barré syndrome raises questions regarding whether these outcomes are caused by genetic mutations or if prior infection by other flaviviruses affects disease outcome. (cdc.gov)
  • The immune system is constituted by a very complex network of cells, tissues, and organs that through soluble factors and direct cell-to-cell contacts interact among themselves and with cells belonging to other (organ) systems. (frontiersin.org)
  • In addition, ORs were recently shown to be expressed in many nonolfactory tissues and cells, indicating that these receptors have physiological and pathophysiological roles beyond olfaction. (bmbreports.org)
  • These results showed that 2D3 cell line was a novel and stable tool useful for the efficient and precise evaluation of the functional avidity of isolated and transduced TCRs in developing TCR-based immunotherapy. (oncotarget.com)
  • Multiple myeloma (MM) is a malignant plasma cell disorder with debilitating symptoms related to anemia, immunosuppression, bone destruction, and renal failure. (haematologica.org)
  • NK cells are innate immune cells crucial for killing of infected and malignant cells. (infinite-science.de)
  • CD19 expression is restricted to normal mature B cells, malignant B cells, and B-cell precursors. (ashpublications.org)
  • These immature stem cells were easier to match to patients, especially those from non-Caucasian ethnicities, and could be stored for use as needed. (biologynews.net)
  • Mitochondrial membrane potential was found at low levels in cells treated with Schiff base compounds and doxorubicin. (ijpsonline.com)
  • The inhibition of cell proliferation and apoptosis on MDA-MB231 and MCF-7 cell lines occurred via the mitochondrial pathway and induction of oxidative damage to DNA through Bax/Bcl-2 modulation, decreased levels of procaspase-8, -9, -3, increased levels of cleaved caspase-3 and ROS, and also cell cycle arrest at S-phase (Jamali et al. (dieselok.md)
  • Further, C.decandratreatment altered the mitochondrial membrane potential in AGS cells. (pdfplayer.org)
  • Another important group of regulators of the K562 line are Sirtuins, referred to as SIRTS. (wikipedia.org)
  • In the present review, we describe and discuss the effects of extremely low frequency (ELF)-EMF and pulsed EMF on cell signals and factors relevant to the activation of danger signals and innate immunity cells. (frontiersin.org)
  • These changes can also drive the leukemic cells to a state of stress, which allows for increased sensitivity of the cells to drugs that initiate apoptosis. (wikipedia.org)
  • Platelets store and secrete the chemokine stromal cell-derived factor (SDF)-1alpha upon platelet activation, but the ability of platelet-derived SDF-1alpha to signal in an autocrine/paracrine manner mediating functional platelet responses relevant to thrombosis and haemostasis is unknown. (bioxcell.com)
  • To evaluate anti-CD19-CAR-transduced T cells in a murine model of adoptive T-cell therapy, we developed a CAR that specifically recognized murine CD19. (ashpublications.org)
  • To establish a murine model in which a completely syngeneic lymphoma could be treated by adoptive transfer of syngeneic CAR-transduced T cells, we developed a CAR that could specifically recognize murine CD19. (ashpublications.org)
  • Thought to be disorders of hematopoietic stem cells, clonal cytogenetic abnormalities are frequently identified. (acgtfoundation.org)
  • Evidence exists that immune activation against hematopoietic elements frequently occurs in MDS patients, based on the identification of lymphocytic infiltrates in the marrow, oligoclonal expansion of T cells, and excessive production of tumor necrosis factor alpha. (acgtfoundation.org)
  • By discussing the EMF modulating effects on cell functions, we envisage the use of EMF as a therapeutic agent to regulate immune responses associated with wound healing. (frontiersin.org)
  • Noteworthy, in recent years the possibility to use EMF exposure to modulate immune cell responses has been proposed and debated ( 6 - 9 ). (frontiersin.org)
  • The IV.3 monoclonal antibody reacts with human CD32 also known as FcγRII and FCRII, a 40 kDa polymorphic transmembrane glycoprotein and an Ig superfamily member. (bioxcell.com)
  • Anti-CD19-CAR-transduced T cells had superior antilymphoma efficacy compared with the anti-CD19 monoclonal antibody from which the anti-CD19 CAR was derived. (ashpublications.org)
  • Results C. butyricum stimulates elevated levels of IL-10 in HT-29 cells To investigate whether C. butyricum regulates IL-10 expression in HT-29 cells, a stimulation assay was performed, as described in the methods. (egfrsignals.com)
  • The same cells from the culture media were collected, and subjected to real-time PCR assay. (egfrsignals.com)
  • Cell viability was measured using the MTT assay. (hindawi.com)
  • Integrin-alpha FG-GAP repeat-containing protein 1 (ITFG1), also known as T cell immunomodulatory protein (TIP), was initially identified using bioinformatics and high-throughput cell-based screening assays to isolate novel factors involved in T cell biology. (rndsystems.com)
  • Eight of these SNPs were analyzed for transcriptional regulation using reporter assays where all tested regions exerted regulatory effects on transcription in epithelial and/or immune cell lines, and seven SNPs showed allelic differences. (biomedcentral.com)
  • In 3-day MTS cell proliferation assays, Saracatinib inhibits proliferation of the Bcr-Abl-driven human leukemia cell line K562 with an IC50 of 0.22 μM. (xcessbio.com)
  • K562 cells were cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum and antibiotics. (mendeley.com)
  • Recombinant full length protein corresponding to Human Retinoid X Receptor beta/RXRB. (abcam.com)
  • 2015). "SDF-1alpha is a novel autocrine activator of platelets operating through its receptor CXCR4" Cell Signal 27(1): 37-46. (bioxcell.com)
  • Feline leukemia virus subgroup C receptor 1 (isoforms during erythropoiesis. (bioerc-iend.org)
  • γδ T cells express unique T cell receptor (TCR) γ and TCR δ chains, with structural and functional heterogeneity. (explorationpub.com)
  • Taking advantage of the diverse γδ TCR repertoire or other ligand-receptor interactions, γδ T cells can recognize a broad spectrum of tumor-associated antigens (TAAs) in a major histocompatibility complex (MHC)-independent manner, thereby activating downstream pleiotropic effects. (explorationpub.com)
  • 1 Significant advances have been made by combining novel agents with autologous peripheral blood stem cell transplantation which allows for long-term disease-free survival in the majority of transplant-eligible patients. (haematologica.org)
  • However, in 1988, researchers found cord blood to be another source for stem cell transplantation. (biologynews.net)
  • Apoptosis is an important mechanism in regulating K562 cells and can be induced by the changes in the metabolic state of the cells. (wikipedia.org)
  • Adenine improves the survival of rat Purkinje cells and enhances the storage of erythrocyte in whole blood [ 6 - 8 ]. (hindawi.com)
  • Of these, single cell RT-qPCR provides combined advantages of sensitivity, specificity, and dynamic range, but is limited by low throughput, high reagent cost, and difficulties in accurately measuring low abundance transcripts (6). (justia.com)
  • The offset of these cellular components from their balance point causes morphological changes, which result in the K562 cells being arrested in the G2/M phase of the cell cycle. (wikipedia.org)
  • These play a role in cellular stress, metabolism, and autophagy, by interacting with deacetylases activity in the cell. (wikipedia.org)
  • Inhibition of cell viability was assessed with focus on apoptosis transcriptionally and translationally. (hindawi.com)
  • According to the current view, damage-associated molecules released by infected or injured cells, or secreted by innate immune cells generate danger signals activating an immune response. (frontiersin.org)
  • This cell line has been recommended as a control cell line for use with the Telomere PNA Kit/FITC for flow cytometry from DakoCytomation. (sigmaaldrich.com)
  • Methods for cell capture and preparation are also provided that include flowing cells through a chamber, funneling the cells towards a cell trap, capturing a predefined number of the cells within the trap, interrupting the flow of cells, flowing a wash solution through the chamber to remove contaminants from the chamber, and sealing the predefined number of cells in the chamber. (justia.com)
  • Quantification of Linoleylethanolamide dsRed+ cells from zebrafish embryos Transgenic embryos expressing green fluorescent protein (GFP) under the control of the promoter and red fluorescent protein (dsRed) under the promoter 16 were dissociated into single cells and analyzed by flow cytometry.14 O-dianisidine staining and heme content For O-dianisidine staining ten embryos/condition were collected and stained according to standard procedure. (bioerc-iend.org)
  • Figure 1A shows that IL-10 concentrations in the media of HT-29 cells cultured with C. butyricum were increased significantly. (egfrsignals.com)
  • The efficacy of the BT-cisplatin combination depends upon the cell type and concentrations of cisplatin and BT. (biomedcentral.com)
  • In cisplatin-sensitive cell lines, BT and cisplatin were mostly antagonistic except when used at low concentrations, where synergy was observed. (biomedcentral.com)
  • In contrast, in cisplatin-resistant cells, BT-cisplatin combination treatment displayed synergistic effects at most of the drug ratios/concentrations. (biomedcentral.com)
  • However, a discussion that started around the 1970s showed the great risk to human health from the generation of a class of substances considered potentially carcinogenic, the nitrosamines, when high nitrite concentrations are exposed to high temperature conditions, as usually occurs in the manufacture of cured meat products, and since then, its use has been considered increasingly controversial [ 2 ][ 12 ] . (encyclopedia.pub)
  • Perturbing agents, such as toxic compounds ( 1 ), ionizing radiation ( 2 ), and some pathogens ( 3 ) can compromise the integrity of the immune system as they damage immune cells and/or irreversibly alter some immune functions. (frontiersin.org)
  • This review further emphasizes the diversified cross-talk between γδ T cells and other immune cells. (explorationpub.com)
  • AVM induced the release of cleaved PARP and cleaved caspase‑3 caused apoptosis and inhibited the viability of these cells. (spandidos-publications.com)
  • In previous research, the authors demonstrated that the methanol extract of A. vulgaris (AVM) has the ability to inhibit the viability of CML cells ( 10 ). (spandidos-publications.com)
  • Concerns on possible detrimental effects of extremely low frequency (ELF)-EMF exposure on human health were raised but, due to contradictory conclusions, no consensus was reached ( 5 ). (frontiersin.org)
  • Conclusions These studies provide the rationale for testing expanded natural killer cells in humans. (haematologica.org)
  • therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. (bdbiosciences.com)
  • Western Blot: SOS1 Antibody (SOS-01) [NB110-68800] - Analysis (reducing conditions) of human Sos using anti-human Sos (SOS-1). (novusbio.com)
  • This review emphasizes the critical role for epidemiologic studies (retrospective and prospective) in combination with the studies to identify specific sites of Zika virus infection in humans that are needed to establish antibody-dependent enhancement as a possibility or a reality. (cdc.gov)
  • Immunocytochemistry/ Immunofluorescence: PARP Antibody (194C1439) - Cleaved [NB100-56599] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. (novusbio.com)
  • Human, CD3+ T cell-depleted PBMCs were expanded in a bioreactor with IL-2 and a glycoprotein antibody cocktail following GMP guidelines. (infinite-science.de)