• The mutation in leptin receptor (LEPR) gene causes splicing abnormality that resulted in truncated receptor, aberrant signal transduction, leptin resistance, and obesity. (ajol.info)
  • Polymorphism of rs1137100 (K109R) and rs1137101 (Q223R) were associated with obesity and leptin level. (ajol.info)
  • In obesity, a decreased sensitivity to leptin occurs (similar to insulin resistance in type 2 diabetes), resulting in an inability to detect satiety despite high energy stores and high levels of leptin. (wikipedia.org)
  • Further studies of leptin, its receptor, and the molecular components of this system are likely to have a major impact on our understanding of obesity and the interplay between nutrition and physiology. (nih.gov)
  • Leptin receptor deficiency is a condition that causes severe obesity beginning in the first few months of life. (medlineplus.gov)
  • Leptin receptor deficiency is a rare cause of obesity. (medlineplus.gov)
  • Andiran N, Celik N, Andiran F. Homozygosity for two missense mutations in the leptin receptor gene (P316:W646C) in a Turkmenian girl with severe early-onset obesity. (medlineplus.gov)
  • Dubern B, Clement K. Leptin and leptin receptor-related monogenic obesity. (medlineplus.gov)
  • Mutations of the ob gene resulting in leptin deficiency are the cause of obesity in the ob/ob mice suggesting that endogenous leptin can normalize body weight. (bertin-bioreagent.com)
  • This mutation results in a mature, non-functional, 81 aa residue, truncated leptin that contributes to the general obesity associated with the ob/ob mouse phenotype. (rndsystems.com)
  • Human obesity is associated with leptin resistance, and recent studies have demonstrated that hypothalamic expression of the suppressors of cytokine signaling 3 (SOCS3) regulates leptin sensitivity in rodents. (mcmaster.ca)
  • These data suggest that this impairment of leptin signaling in skeletal muscle may contribute to the aberrant regulation of fatty acid metabolism observed in obesity and that pharmacological activation of AMPK may be an effective therapy to bypass SOCS3-mediated skeletal muscle leptin resistance for the treatment of obesity-related disorders. (mcmaster.ca)
  • Leptin is one of the major adipokines in obesity that indicates the severity of fat accumulation. (plos.org)
  • Aging has been demonstrated to aggravate obesity and to induce leptin resistance and hyperleptinemia. (plos.org)
  • We aimed to review the literature on the effects of training intervention on peripheral leptin level in obesity during aging, in order to evaluate the independent efficacy of this method. (plos.org)
  • Moreover, the elevated LEP expression observed in obesity correlated well with increased FOSL2 levels in mice and humans, and adipocyte-specific genetic deletion of Fosl2 in mice reduced Lep expression. (evanrosenlab.com)
  • Different concentrations of hormones, such as ghrelin, leptin and insulin during foetal life raises the question whether or not they can be modulated, thereby avoiding obesity before birth. (aaem.pl)
  • Because cardiovascular and inflammatory stress responses are predictive of future cardiovascular disease, leptin may be a mechanism mediating the adverse effects of stress and obesity on women's cardiovascular health. (vu-ams.nl)
  • Mastering the fat hormone leptin is the single most important factor in preventing obesity, diabetes, and heart disease. (wellnessresources.com)
  • Studies have investigated the role of leptin in obesity, anorexia nervosa, insulin resistance, and hypertension. (thermofisher.com)
  • Severe hereditary obesity in rodents and humans can be caused by defects in leptin production. (thermofisher.com)
  • Some studies have linked intake of a GF diet to reduced obesity and T2D and suggested a role in reducing leptin- and insulin-resistance and increasing beta-cell volume. (mdpi.com)
  • The long-term clinical outcomes of severe obesity due to leptin signaling deficiency are unknown. (bvsalud.org)
  • The analysis included whole-exome sequencing data of 366 children with severe obesity, 1,000 individuals of the Pakistan Risk of Myocardial Infarction Study (PROMIS) study, and 200,000 participants of the UK Biobank to prioritize genes harboring rare homozygous variants with putative effect on human obesity. (bvsalud.org)
  • [ 10 ] Mutations of genes encoding either leptin or the leptin receptor underlie isolated cases of autosomally transmitted idiopathic hypogonadotropic hypogonadism associated with early-onset obesity. (medscape.com)
  • In humans, low circulating plasma leptin has been associated with cognitive changes associated with anorexia, depression, and Alzheimer's Disease. (wikipedia.org)
  • Weight gain results in an increased plasma leptin level, which elicits a biologic response characterized in part by a state of negative energy balance. (nih.gov)
  • Plasma leptin, insulin, and neuropeptide Y concentrations in infants. (aaem.pl)
  • In women only, baseline plasma leptin was significantly associated with stress-induced changes in heart rate (β = 0.53, P = 0.006), heart rate variability (HRV) (β = −0.44, P = 0.015), and cardiac preejection period (PEP) (β = −0.51, P = 0.004), independent of age, adiposity, and smoking. (vu-ams.nl)
  • Women's plasma leptin levels also correlated with stress-induced elevations in the proinflammatory cytokine interleukin-6 (IL-6) (β = 0.35, P = 0.042). (vu-ams.nl)
  • For that purpose, daily variations of plasma leptin, cortisol, insulin and glucose were analyzed in pinealectomized hamsters, as compared to sham-operated controls kept under very long (16 h light/08 h dark) or short photoperiods (08 h light/16 h dark). (frontiersin.org)
  • Together, these findings in Syrian hamsters show that circulating melatonin at night drives the daily rhythmicity of plasma leptin, participates in the phase control of cortisol rhythm and modulates glucose homeostasis according to photoperiod-dependent metabolic state. (frontiersin.org)
  • Plasma leptin is secreted by adipocytes in proportion with adiposity ( Ahima and Flier, 2000 ). (frontiersin.org)
  • Leptin receptors are expressed by a variety of brain and peripheral cell types. (wikipedia.org)
  • Although regulation of fat stores is deemed to be the primary function of leptin, it also plays a role in other physiological processes, as evidenced by its many sites of synthesis other than fat cells, and the many cell types beyond hypothalamic cells that have leptin receptors. (wikipedia.org)
  • Mode of action Leptin acts directly on leptin receptors in the cell membrane of different types of cells in the human body in particular, and in vertebrates in general. (wikipedia.org)
  • Thus some leptin receptors in the brain are classified as central (hypothalamic) and some as peripheral (non-hypothalamic). (wikipedia.org)
  • Leptin is involved in regulating food intake, energy expenditure, and adiposity through hypothalamic leptin receptors. (thermofisher.com)
  • As scientifically known so far, the general effects of leptin in the central nervous system are: Deficiency of leptin has been shown to alter brain proteins and neuronal functions of obese mice which can be restored by leptin injection. (wikipedia.org)
  • Objective: The objective of the study was to investigate the effects of leptin on fatty acid oxidation and AMPK signaling in primary myotubes derived from lean and obese skeletal muscle and evaluate the contribution of SOCS3 to leptin resistance and AMPK signaling in obese humans. (mcmaster.ca)
  • Gain- and loss-of-function experiments in vivo confirm the role of IRF3 in mediating the acute satiety-evoking effects of leptin in AgRP neurons, while live-cell imaging in vitro indicate that leptin can activate neuronal IRF3 in a cell autonomous manner. (biorxiv.org)
  • Thus, our findings identify AgRP neuron-expressed IRF3 as a key transcriptional effector of the hunger-suppressing effects of leptin. (biorxiv.org)
  • and primary vs secondary is an arbitrary description of a particular function of leptin. (wikipedia.org)
  • The hyperphagia, low sympathetic nervous system tone, and decreased circulating concentrations of bioactive thyroid hormones that are common to states of congenital leptin deficiency and hypoleptinemia following and during weight loss suggest that the major physiological function of leptin is to signal states of negative energy balance and decreased energy stores. (endocrinology-journals.org)
  • The major physiological function of leptin is to signal states of negative energy balance and decreased energy stores. (endocrinology-journals.org)
  • This study aims to determine the association of LEPR gene polymorphisms, rs1137100 and rs1137101, on phenotype and leptin level between obese and control groups in Yogyakarta population. (ajol.info)
  • Leptin receptor deficiency is caused by mutations in the LEPR gene. (medlineplus.gov)
  • LEPR gene mutations that cause leptin receptor deficiency prevent the receptor from responding to leptin, leading to the excessive hunger and weight gain associated with this disorder. (medlineplus.gov)
  • Because hypogonadotropic hypogonadism occurs in leptin receptor deficiency, researchers suggest that leptin receptor signaling is also involved in regulating the body's response to hormones that control sexual development, and that this response is affected by LEPR gene mutations. (medlineplus.gov)
  • We have previously reported that a single nucleotide polymorphism (SNP) in leptin receptor (LEPR), present in up to 50% of people, influenced CDI-induced neutrophil CXCR2 expression and tissue neutrophilia. (frontiersin.org)
  • We carry out a retrospective cross-sectional investigation of a large cohort of children with leptin (LEP), LEP receptor (LEPR), or melanocortin 4 receptor (MC4R) deficiency (n = 145) to evaluate the progression of the disease. (bvsalud.org)
  • Context: Leptin is thought to regulate whole-body adiposity and insulin sensitivity, at least in part, by stimulating fatty acid metabolism via activation of AMP-kinase (AMPK) in skeletal muscle. (mcmaster.ca)
  • These findings suggest that specific MFN2 mutations cause tissue-selective mitochondrial dysfunction with increased adipocyte proliferation and survival, confirm a novel form of excess adiposity with paradoxical suppression of leptin expression, and suggest potential targeted therapies. (univpm.it)
  • Soluble Leptin receptor levels are indirectly proportional to adiposity and are increased in females versus males. (biovendor.com)
  • This suggests that excessive adiposity overrides the absence of leptin to promote a low-grade inflammatory environment in these tissues. (peerj.com)
  • Leptin is a hormone that regulates homeostasis energy through the central- peripheral mechanism as well as regulates hunger and satiety. (ajol.info)
  • Leptin (from Greek λεπτός leptos, "thin" or "light" or "small") is a protein hormone predominantly made by adipose cells and its primary role is likely to regulate long-term energy balance. (wikipedia.org)
  • Predominantly, the "energy expenditure hormone" leptin is made by adipose cells, and is thus labeled fat cell-specific. (wikipedia.org)
  • Leptin is an adipocyte hormone that functions as the afferent signal in a negative feedback loop regulating body weight. (nih.gov)
  • The leptin receptor is turned on (activated) by a hormone called leptin that attaches (binds) to the receptor, fitting into it like a key into a lock. (medlineplus.gov)
  • Leptin, the "satiety hormone", is a hormone made by adipose cells that helps to regulate energy balance by inhibiting hunger. (transcriptionfactor.org)
  • The adipocyte-derived hormone leptin is a critical regulator of many physiological functions, ranging from satiety to immunity. (evanrosenlab.com)
  • The adipocyte hormone leptin, which stimulates energy expenditure in animals by activating the sympathetic nervous system (SNS), is believed to play a role in this association. (vu-ams.nl)
  • The hormone leptin is intricately involved in the regulation of appetite, metabolism and calorie burning. (howstuffworks.com)
  • The Leptin Diet contains five simple lifestyle guidelines to get the hormone leptin into balance for permanent weight loss, increased energy, and optimum health. (wellnessresources.com)
  • Fat cells produce the powerful hormone leptin, a primary force instructing metabolism, weight management, and hormone balance. (wellnessresources.com)
  • In 2002 he authored the breakthrough book, Mastering Leptin , the first book to explain the hormone leptin to the public. (wellnessresources.com)
  • Leptin is a 167 amino acid long protein hormone with important effects in regulating body weight, metabolism and reproductive function. (thermofisher.com)
  • Leptin is a peptide hormone produced by adipocytes that has anorexigenic effects upon appetite while regulating immunity. (peerj.com)
  • People with leptin receptor deficiency also have hypogonadotropic hypogonadism, which is a condition caused by reduced production of hormones that direct sexual development. (medlineplus.gov)
  • Based on the weight loss induced by leptin administration in states of leptin deficiency (obese) and observed similarity of phenotypes in states of congenital and dietary-induced states of hypoleptinemia (reduced obese), it has been suggested that exogenous leptin could potentially be useful in initiating, promoting, and sustaining weight reduction. (endocrinology-journals.org)
  • Our aim was to study the localization of LPL and its endothelial anchoring protein glycosylphosphatidylinositol-anchored high density lipoprotein-binding protein 1 (GPIHBP1) in mouse pancreas, and effects of diet and leptin deficiency on their expression patterns. (columbia.edu)
  • Conversely, leptin deficiency decreased pro-inflammatory cytokine gene expression in heart (IL-1β, TNF-α). (peerj.com)
  • Taken together, these findings suggest that leptin deficiency affects cytokine gene expression differently in the brain compared to peripheral tissues with minimal interaction from acute SF. (peerj.com)
  • Lyophilized Pegylated Leptin N82K although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Pegylated Leptin N82K should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). (prospecbio.com)
  • Recombinant Leptin is a monomer protein consisting of 147 amino acid residue subunits, and migrates as an approximately 16 kDa protein under reducing conditions in SDS-PAGE. (reprokine.com)
  • This gene provides instructions for making a protein called the leptin receptor, which is involved in the regulation of body weight. (medlineplus.gov)
  • A cDNA sequence encoding the sequence of Leptin tA was constructred and used to recombinantly synthesize the protein. (enquirebio.com)
  • Named leptin (from leptos , Greek for thin) because of its ability to reduce fat stores, the protein shows high interspecies conservation, with mature human leptin being 87% and 84% identical to mouse and rat leptin proteins, respectively. (rndsystems.com)
  • Leptin is frequently also referred to as OB protein, the product of the ob (obese) gene. (rndsystems.com)
  • Rat leptin shares approximately 96% and 82% sequence identity with the mouse and human protein, respectively. (reliatech.de)
  • Leptin receptor (OB R) was identified as a leptin binding protein (Leptin, the product of the ob gene, is a single chain 16 kDa protein consisting of 146 amino acid residues. (biovendor.com)
  • This second base substitution changed a glutamine to an arginine at position 223 of the leptin receptor protein. (johnshopkins.edu)
  • Leptin might be what pnoeric was thinking of, but it's not "protein from a rodent. (food52.com)
  • Association of the fibronectin type III domain-containing protein 5 rs1746661 single nucleotide polymorphism with reduced brain glucose metabolism in elderly humans. (cdc.gov)
  • In humans, low protein intake by women in late but intrauterine exposure was (20). (cdc.gov)
  • Observational studies comparing circulating leptin levels between COPD patients and healthy controls were included. (karger.com)
  • Circulating leptin levels were correlated with the body mass index (BMI) as well as percent fat mass in stable COPD patients. (karger.com)
  • Most studies indicated that circulating leptin levels in stable COPD patients were not significantly different from those in healthy controls when adjusted for gender and BMI, whilst leptin levels tended to elevate in exacerbation groups. (karger.com)
  • A mutation in the ob gene has been found in mice, but not in humans. (rndsystems.com)
  • Here, we report studies in which we pursued a strategy integrating BAC transgenic reporter mice, reporter assays, and chromatin state mapping to locate an adipocyte-specific cis-element upstream of the leptin (LEP) gene in human fat cells. (evanrosenlab.com)
  • For this, immunofluorescence microscopy was used on pancreatic tissue from C57BL/6 mouse embryos (E18), adult mice on normal or high-fat diet, and adult ob/ob-mice treated or not with leptin. (columbia.edu)
  • Leptin treatment of ob/ob mice for 12 days reversed this pattern, so that most of the islets expressed LPL in beta cells. (columbia.edu)
  • Produced in BALB/c mice using recombinant human Leptin as the immunizing antigen. (reliatech.de)
  • Mice with mutations in the obese gene that block the synthesis of leptin are obese and diabetic, and have reduced activity, metabolism, and body temperature. (reliatech.de)
  • Additionally, administration of a clinically available GHR antagonist (pegvisomant) attenuates the fall of whole-body energy expenditure of food-deprived mice, similarly as seen by leptin treatment. (nih.gov)
  • The mutation responsible for the defect in the leptin receptor in db/db mice was not detected in any obese human, nor was the fa/fa rat mutation. (johnshopkins.edu)
  • Male C57BL/6j (lean) and ob/ob mice (leptin-deficient mice exhibiting obese phenotype) were subjected to SF or control conditions for 24 h using an automated SF chamber. (peerj.com)
  • The objective of the study was to investigate the potential association of human milk leptin concentrations with child body mass index (BMI) and BMI trajectory patterns up to two years of age among children in the Ulm SPATZ Health Study. (uni-ulm.de)
  • Leptin, the product of the ob (obese) gene, is produced mainly in the adipose tissue, and is considered to play an important role in appetite control, fat metabolism and body weight regulation. (bertin-bioreagent.com)
  • This enables Leptin to then yoke metabolism and sleep directly to the light and dark cycles of our day. (jackkruse.com)
  • The decrease in leptin brought on by sleep deprivation can result in a constant feeling of hunger and a general slow-down of your metabolism. (howstuffworks.com)
  • Leptin problems are the primary reason for food cravings, overeating, faulty metabolism, the obsession with food, and heart disease. (wellnessresources.com)
  • Knockdown of FOSL2 in human adipocytes decreased LEP expression, and overexpression of Fosl2 increased Lep expression in mouse adipocytes. (evanrosenlab.com)
  • Taken together, these data identify FOSL2 as a critical regulator of leptin expression in adipocytes. (evanrosenlab.com)
  • Leptin is expressed predominantly by adipocytes, which fits with the idea that body weight is sensed as the total mass of fat in the body. (thermofisher.com)
  • The normal regulatory mechanism of leptin is maintained in stable COPD patients despite weight loss. (karger.com)
  • High leptin levels are interpreted by the brain that energy reserves are high, whereas low leptin levels indicate that energy reserves are low, in the process adapting the organism to starvation through a variety of metabolic, endocrine, neurobiochemical, and behavioral changes. (wikipedia.org)
  • In this state of weight stability but reduced energy stores, leptin at least partially reverses many of the metabolic, autonomic, neuroendocrine, and behavioral adaptations that favor weight regain. (endocrinology-journals.org)
  • Weight loss triggers important metabolic responses to conserve energy, especially via the fall in leptin levels. (nih.gov)
  • Both leptin and adiponectin can modulate metabolic processes. (cdc.gov)
  • Gln223Arg) Polymorphism and Circulating Leptin in Patients with Metabolic Syndrome in the Indian Population. (cdc.gov)
  • Training intervention leads to a decrease in leptin level of middle-aged or older, overweight or obese male and female groups, even without major weight loss, indicated by unchanged serum adiponectin levels. (plos.org)
  • Resistance training appears to be more efficient in reducing blood leptin level than aerobic training alone. (plos.org)
  • These results provide evidence that the leptin resistance observed in obese humans is not due to a defect in the leptin receptor. (johnshopkins.edu)
  • 2003). Limited human trials suggested that dietary fructose may or may not induce insulin resistance (Elliott et al. (ift.org)
  • Leptin is an important regulator of food intake and energy expenditure. (karger.com)
  • As one of the major signals of energy status, leptin levels influence appetite, satiety, and motivated behaviors oriented towards the maintenance of energy reserves (e.g., feeding, foraging behaviors). (wikipedia.org)
  • Instead, fructose consumption studies, with either human subjects or animal models, had fructose: glucose ratios nearly four times that typically observed in the U.S. food supply. (ift.org)
  • Leptin takes an important part in the regulation of energy balance and body weight control.After entering the circulation, Leptin binds LEPRwhich results in the activation of several major signalling pathways. (prospecbio.com)
  • Leptin, and pharmacotherapies affecting leptin signaling pathways, is likely to be most useful in sustaining weight loss. (endocrinology-journals.org)
  • Leptin also has thermogenic actions and regulates enzymes of fatty acid oxidation. (thermofisher.com)
  • Soluble Leptin receptor is also found upregulated in patients with chronic heart failure, end-stage renal disease and anorexia. (biovendor.com)
  • Adipokines including leptin, soluble leptin receptor (sOB-R), free leptin index (FLI, the ratio of leptin to sOB-R), total and high molecular weight (HMW) adiponectin were assessed in 2002-2003. (cdc.gov)
  • Human Leptin is generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 16 kDa. (reprokine.com)
  • Leptin is approximately 16 kDa in mass and encoded by the obese (ob) gene. (thermofisher.com)
  • Non-hypothalamic targets of leptin are referred to as peripheral targets. (wikipedia.org)
  • Leptin-receptor gene expression in hypothalamic tissue from lean and obese humans was examined. (johnshopkins.edu)
  • The presence of ghrelin in the stomach of human foetuses and the distinctive production in the pancreas of neonates suggests the role of ghrelin in pre- and post-natal development. (aaem.pl)
  • The ghrelin cell: a novel developmentally regulated islet cell in human pancreas. (aaem.pl)
  • Ghrelin endocrine cells in the human stomach during prenatal and early postnatal development. (aaem.pl)
  • The purpose of ghrelin is basically the exact opposite of leptin: It tells your brain when you need to eat, when it should stop burning calories and when it should store energy as fat. (howstuffworks.com)
  • With shorter nights comes less sleep, less leptin and more ghrelin, making the body eat as much as possible and save those calories for the long winter ahead. (howstuffworks.com)
  • With winter comes more sleep, meaning more leptin and less ghrelin, both of which tell the body it's time to burn those calories it stored during the summer. (howstuffworks.com)
  • 2002) and modulate circulating insulin, leptin, and ghrelin (Teff et al. (ift.org)
  • Among adipokines, leptin and adiponectin are the most abundantly secreted peptides [ 11 ]. (plos.org)
  • Weight loss among both lean and obese subjects results in decreased plasma levels of leptin, which lead to a state of positive energy balance and a number of other physiologic responses. (nih.gov)
  • Results: We demonstrate that leptin stimulates AMPK activity and increases AMPK Thr172 and acetyl-CoA carboxylase-β Ser222 phosphorylation and fatty acid oxidation in lean myotubes but that in obese subjects leptin-dependent AMPK signaling and fatty acid oxidation are suppressed. (mcmaster.ca)
  • Overexpression of SOCS3 via adenovirus-mediated infection in lean myotubes to a similar degree as observed in obese myotubes prevented leptin but not AICAR (5-amino-imidazole-4-carboxamide-1-β-d-ribofuranoside) activation of AMPK signaling. (mcmaster.ca)
  • We describe similar massive adipose overgrowth with suppressed leptin expression in four further patients with biallelic MFN2 mutations and at least one p.Arg707Trp allele. (univpm.it)
  • We also identified for the first time in the human, two homozygous stop-gain mutations in ASNSD1 and IFI16 genes. (bvsalud.org)
  • Recent research has revealed that leptin can induce expression of a neuropeptide called vasoactive intestinal peptide (VIP) through the VIP cytokine response element. (jackkruse.com)
  • Human peripheral blood was treated with BD Pharm Lyse™ Lysing Buffer (Cat. (bdbiosciences.com)
  • There is a different relative importance of central and peripheral leptin interactions under different physiologic states, and variations between species. (wikipedia.org)
  • Human adiponectin (aa 16-244) is fused at the N-terminus to a linker peptide (14 aa) and a FLAG ® -tag. (enzolifesciences.com)
  • In humans, both the intrinsic sensitivity to leptin and its rate of production vary and both appear to contribute to differences in weight. (nih.gov)
  • Leptin (from the Greek word Leptos -- meaning thin)/ Orchestrates the use of fats to help the body: Accelerate fat breakdown, Control appetite and sweet cravings, Promote fat loss, Regulate pituitary and thyroid functions, Influence fat cell size. (food52.com)
  • Leptin is secreted in circadian fashion with nocturnal rise in both lean and obese patients. (bertin-bioreagent.com)
  • Today, I will give you a very cursory review of why circadian biology, leptin, and environment are critical to using the Quilt to obtain your Optimal life. (jackkruse.com)
  • Why is circadian biology critical to humans? (jackkruse.com)
  • Most people know that the suprachiasmatic nucleus (SCN) in the brain is where the circadian pacemaker lies in humans. (jackkruse.com)
  • This means that leptin uncouples the SCN to be unable use light as the main stimulus to yoke circadian cycles. (jackkruse.com)
  • Once temperature becomes the dominant method of entrainment used to yoke the circadian rhythms to leptin, some very unique things happen to our biochemistry that normally do not occur in other environments. (jackkruse.com)
  • In addition, leptin is rhythmically secreted, with peak phases depending on the species. (frontiersin.org)
  • It includes more than 200 species found in diverse ecosystems, including the human body and fermented dairy products 1 . (nature.com)
  • Leptin concentration was measured in skimmed human milk by ELISA (R&D System). (uni-ulm.de)
  • Sandwich ELISA: To detect Rat Leptin by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. (thermofisher.com)
  • Western Blot: To detect Rat Leptin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. (thermofisher.com)
  • When temperature becomes the dominant environmental trigger and not light cycles, leptin induces endothelial nitric oxide synthetase (eNOS) that shuts down the photic effects of VIP on the SCN. (jackkruse.com)
  • Anti-Rat Leptin-specific antibody was purified by affinity chromatography employing an immobilized Rat Leptin matrix. (thermofisher.com)
  • Cross selling for this product : Leptin (human) ELISA kit There are 10 products. (bertin-bioreagent.com)
  • BioVision's Leptin ELISA kit is a sandwich ELISA assay for the quantitative measurement of Leptin in human serum, plasma and cell culture supernatants in 90 minutes. (transcriptionfactor.org)
  • This ELISA kit is used for quantitative measurement of total Leptin in human serum, plasma, urine, cell culture samples, biological fluid. (transcriptionfactor.org)
  • Leptin receptor may act as a negative regulator of Leptin activity and it may maintain a pool of available bioactive Leptin by binding and delaying its clearance from circulation. (biovendor.com)
  • Leptin administration to humans at usual weight has little, if any, effect on body weight while leptin administration during weight loss mitigates hunger, especially if given in supraphysiological doses during severe caloric restriction. (endocrinology-journals.org)
  • During sleep, leptin levels increase, telling your brain you have plenty of energy for the time being and there's no need to trigger the feeling of hunger or the burning of calories. (howstuffworks.com)
  • Here we generated AgRP neuron-specific transcriptomic and chromatin accessibility profiles during opposing states of fasting-induced hunger and leptin-induced hunger suppression. (biorxiv.org)
  • Cis-regulatory analysis of these integrated datasets enabled the identification of 28 putative hunger-promoting and 29 putative hunger-suppressing transcriptional regulators in AgRP neurons, 16 of which were predicted to be transcriptional effectors of leptin. (biorxiv.org)
  • Within our dataset, Interferon regulatory factor 3 (IRF3) emerged as a leading candidate mediator of leptin-induced hunger-suppression. (biorxiv.org)
  • In humans, leptin levels correlate with body mass index (BMI) and percentage body fat, and are elevated even in obese individuals. (bertin-bioreagent.com)
  • A positive correlation between leptin and tumor necrosis factor (TNF)-α levels was found in COPD exacerbations, while it disappeared in patients with stable disease. (karger.com)
  • Leptin receptor levels are highest in infants, decrease into adolescence, and remain relatively stable throughout adulthood. (biovendor.com)
  • Conclusion: Some PFAS may alter circulating levels of leptin. (cdc.gov)
  • Leptin, insulin, and cortisol levels were measured by ELISA. (bvsalud.org)
  • There was no difference in the amount of leptin-receptor mRNA in seven lean (BMI 23.3 ± 0.9 kg/m 2 ) and eight obese (BMI 36.9 ± 1.5) subjects as determined by reverse transcription-polymerase chain reaction. (johnshopkins.edu)