• Overexpression of RGPR-p117 did not cause an alteration of cell proliferation and led to significant decreases in protein and DNA contents in cloned normal rat kidney proximal tubular epithelial NRK52E cells. (wikipedia.org)
  • RON is activated in response to macrophage-stimulating protein (MSP), and then induces an invasive program ( 8 ) consisting of cell proliferation, migration, and invasion, all of which are important at multiple points during tumorigenesis. (spandidos-publications.com)
  • activation of NF-κB and costimulation of T cell proliferation. (biolegend.com)
  • Cell proliferation and invasion were assayed using the kidney cell lines HEK293 with wild-type p53 and a ccRCC cell line MZ1257RC mutated for p53. (frontiersin.org)
  • HEK293 cells demonstrated a restriction of ERV-K113 env expression and invasion with no changes in proliferation in the absence of Aza. (frontiersin.org)
  • The Wnt family encodes a large group of signaling molecules that are involved in cell proliferation, differentiation, migration and apoptosis [ 13 , 14 ]. (oncotarget.com)
  • Here we identify CSNK1E , the gene encoding casein kinase 1 epsilon (CK1ε) as required specifically for the proliferation of breast cancer cells with activated β-catenin and confirm its role as a positive regulator of β-catenin-driven transcription. (plos.org)
  • The Wnt/β-catenin pathway plays a critical role in embryonic development, maintenance of multipotent progenitor cell populations and proliferation of many tissue types [1] , [2] . (plos.org)
  • The anti-proliferation effect (cytotoxicity) of Ad was assessed using the colorimetry-based MTT cell viability assay. (biomedcentral.com)
  • In turn, the addition of Ad-Endo enhanced the inhibitory effect of dl 1520 on the proliferation of GC cells. (biomedcentral.com)
  • Hepatocyte growth factor (HGF) is produced by stromal and mesenchymal cells, and it stimulates epithelial cell proliferation, motility, morphogenesis and angiogenesis in various organs via tyrosine phosphorylation of its cognate receptor, Met. (spandidos-publications.com)
  • Circle RNA circCSPP1 promotes human osteosarcoma cell proliferation and increases glucose metabolism by suppressing miR-200c maturation. (nih.gov)
  • In response to mito- pendence between the key mecha- gens, cell proliferation is triggered by nistic characteristics. (who.int)
  • H - Ras cell proliferation are also linked with haematopoietic cel s. (who.int)
  • Maturation occurs in the cell membrane resulting in a 180-kDa heterodimeric protein composed of a 40-kDa α-chain and a 150-kDa transmembrane β-chain with intrinsic tyrosine kinase activity ( 5 , 6 ). (spandidos-publications.com)
  • A urate/anion exchanger (URAT1) has been identified in the brush-border membrane of the kidneys and is inhibited by an angiotensin II receptor blocker, losartan. (medscape.com)
  • NPHS2 is almost exclusively expressed in the podocytes of fetal and mature kidney glomeruli, and encodes a new integral membrane protein, podocin, belonging to the stomatin protein family. (scienceopen.com)
  • We propose the outline of such a mechanism based on following aspects: (1) The glomerulus is a complex structure, the stability of which depends on the cooperative function of the basement membrane, mesangial cells and podocytes, counteracting the distending forces originating from the high glomerular hydrostatic pressures. (scienceopen.com)
  • The RMT2-26 monoclonal antibody specifically recognizes T cell immunoglobulin and mucin domain-2 (TIM-2) which is also known as T-cell membrane protein 2, TIMD-2, or Tim2. (bdbiosciences.com)
  • Epithelial cells express a number of characteristic proteins and glycoproteins like cytokeratins, epithelial membrane antigen and epithelium-specific glycoprotein Egp34 (HEA125). (exalpha.com)
  • All AE proteins are hypothesized to share a similar topology in the cell membrane. (tcdb.org)
  • AE1, AE2, and NBCn1), (ii) is important for trafficking to the cell membrane (e.g. (tcdb.org)
  • Ultrastructural analysis of infected Vero cells showed the virions within cell vesicles and around the cell membrane. (cdc.gov)
  • The hallmarks of EMT are characterized by loss of epithelial adhesion molecule E-cadherin and gain of mesenchymal markers such as N-cadherin, vimentin, et al . (biomedcentral.com)
  • CD324 functions as a cell adhesion molecule involved in development, bacterial pathogenesis, and tumor invasion. (biolegend.com)
  • 2003) The epithelial cell adhesion molecule (Ep - CAM) as a morphoregulatory molecule is a tool in surgical pathology. (exalpha.com)
  • IHC-P: Human lung and HEK-293T (human embryonic kidney epithelial cell) transfected with a SCGB1A1 expression vector containing a his tag tissues. (abcam.com)
  • Tissue culture commonly refers to the culture of animal cells and tissues, with the more specific term plant tissue culture being used for plants. (wikipedia.org)
  • [12] He suggested that the potentialities of individual cells via tissue culture as well as that the reciprocal influences of tissues on one another could be determined by this method. (wikipedia.org)
  • The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
  • 2. Momburg F., Moldenhauer G., Hämmerling G.J., and Möller F. (1987) Immunohistochemical study of the expression of a Mr 34,000 human epithelium-specific surface glycoprotein in normal and malignant tissues. (exalpha.com)
  • We have also been studying mechanisms by which oxalate crystals induce inflammation and thereby cause damage to the kidney and other tissues. (yale.edu)
  • One approach involves the generation of isoform- and phospho-specific polyclonal and monoclonal antibodies to identify the cellular and subcellular sites of expression of ion exchangers in the kidney and other tissues, and to study their regulation. (yale.edu)
  • This finding in turn has motivated studies on the mechanisms and regulation of oxalate transporters and their roles in oxalate homeostasis, urolithiasis, and crystal-induced inflammation in the kidney and other tissues. (yale.edu)
  • HGF is produced and secreted by adjacent stromal and mesenchymal cells, it contributes to the development of epithelial organs in a paracrine fashion, exerts regenerative effects on epithelia in the liver, kidney, lung, and other tissues, and promotes the regression of fibrosis in numerous organs ( 7 , 8 ). (spandidos-publications.com)
  • As noted above, the AE or solute carrier 4 (SLC4) family includes 10 members (SLC4A1-5, SLC4A7-11), which are expressed in multiple tissues in the human body. (tcdb.org)
  • In situ hybridization showed that mRNA encoding the reptile homologue of NHE-1 was expressed predominantly in the epithelial cells of these tissues. (arizona.edu)
  • We reviewed published microarray and RNA sequencing studies in which gene expression profiles were compared between human RB and normal retina tissues. (molvis.org)
  • Expression of DCT was absent or barely detected in retina tissues, various human ocular cells, and major organs. (molvis.org)
  • Among these features are the peculiar tropism of the virus for Vero cells (a continuous cell line established from monkey kidney epithelial cells), its capacity for growth at 37°C (while other respiratory coronaviruses grow at lower temperatures), and its ability to infect lower respiratory tract tissues ( 13 ). (cdc.gov)
  • Phosphorylated RGPR-p117 specifically binds to the TTGGC motif in the promoter region of various genes to enhance the gene expression of various proteins, and plays a crucial role as a transcription factor in the cells. (wikipedia.org)
  • Briefly, eight RNA polymerase I plasmids (for the synthesis of the eight influenza A viral RNAs) together with plasmids for the expression of the PB2, PB1, PA, and NP proteins derived from the influenza A virus strain A/WSN/33 (H1N1) were transfected into 293T cells. (cdc.gov)
  • Moreover, they infect cells with viruses, or express individual viral proteins, to follow the virus's actions and the host cell response. (the-scientist.com)
  • CD324 binds to the α E β 7 integrin to mediate cell adhesion and also interacts with a number of intracellular proteins including including erbin, ezrin, caspase-3, caspase 8, β-catenin, presenilin 1, casein kinase II , as well as other extracellular proteins including the EGF receptor. (biolegend.com)
  • We found that "knockout" mice lacking one of these transport proteins have a high incidence of calcium oxalate urinary stones, the same type that is most common in human patients with kidney stones. (yale.edu)
  • Their N-terminal hydrophilic domains may interact with cytoskeletal proteins and therefore play a cell structural role. (tcdb.org)
  • In humans, the AE family (also called the SLC4 family) is composed of 10 paralogous members, among which are the proteins that perform Na + -independent Cl-HCO 3 - exchange (e.g. (tcdb.org)
  • 2007). These proteins are important for the regulation of intracellular pH (pH i ) and play crucial roles in the epithelial absorption of HCO 3 - (e.g., in the renal proximal tubule) and secretion of HCO 3 - (e.g., in the pancreatic duct). (tcdb.org)
  • The primary barriers for invading respiratory pathogens are the respiratory tract epithelial cells and antimicrobial proteins generated by these cells. (cdc.gov)
  • Baby hamster kidney (BHK) cells and fibroblasts from chick embryos are also a good bet, says Richard Condit , an emeritus professor at the University of Florida in Gainesville. (the-scientist.com)
  • We investigated the swelling-induced chloride current in fibroblasts, which we demonstrated is closely related or identical to a cloned epithelial chloride channel, I Cln . (biomedcentral.com)
  • Electrophysiological, fluorescence optical, and volume measurements were made to determine the effect of nucleoside analogs on the swelling-dependent chloride current (I Cl ) in NIH 3T3 fibroblasts and in human T cell lymphoma (H9) cells and the cAMP-dependent chloride current in CaCo cells. (biomedcentral.com)
  • AZT and acyclovir block the swelling-dependent chloride current and the chloride flux in fibroblasts, and the regulatory volume decrease (RVD) and I Cl in H9 cells. (biomedcentral.com)
  • The whole-cell voltage clamp method ( 6 ) was chosen to measure swelling-induced chloride currents in isolated NIH 3T3 fibroblasts, colon carcinoma (CaCo) cells, and H9 cells, a human T cell lymphoma cell line (ATCC HTB 176). (biomedcentral.com)
  • Fibroblasts and CaCo cells were grown on glass cover slips in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal calf serum, 4 mM glutamine, 100 IU/ml penicillin, 100 µ g/ml streptomycin at 37°C, 5% CO 2 , 95% air, and measured 24-48 hr after splitting. (biomedcentral.com)
  • Human CMV grows only in human cells and replicates best in human fibroblasts. (medscape.com)
  • The liver is a major site of metabolism of many endogenous compounds and xenobiotics since hepatocytes (which comprise 80% of the liver cells) contain large amounts of smooth endoplasmic reticulum, where many metabolizing enzymes reside. (aspetjournals.org)
  • TIM-2 is reportedly expressed on B cells with higher levels on activated B cells and germinal center B cells, type-2 T helper (Th2) cells, oligodendrocytes, and epithelial cells in the kidney and liver. (bdbiosciences.com)
  • 80% identical to mammalian NHE-1 homologues at the nucleotide level and recognized a transcript (~5.8-6.0 kb) in RNA isolated from turtle colon, small intestine, stomach, kidney, urinary bladder, heart, and liver. (arizona.edu)
  • Prostate-specific antigen (PSA) is a protein produced by normal prostate cells. (medscape.com)
  • Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Multi Cytokeratin Mouse Monoclonal Antibody (C11). (neobiotechnologies.com)
  • Immunofluorescence Analysis of HeLa cells labeling Multi Cytokeratin with Multi Cytokeratin Mouse Monoclonal Antibody (C11) conjugated with CF640R(Green). (neobiotechnologies.com)
  • This is a broad-spectrum antibody, which has been reported to differentiate epithelial tumors from non-epithelial tumors. (neobiotechnologies.com)
  • In this investigation a human RCC tissue microarray (TMA) (n=374) predominantly consisting of the most common histological tumor subtypes was hybridized with an ERV-K env antibody and correlated with patient clinical data. (frontiersin.org)
  • Burioni, Roberto title: A Non-VH1-69 Heterosubtypic Neutralizing Human Monoclonal Antibody Protects Mice against H1N1 and H5N1 Viruses date: 2012-04-04 journal: PLoS One DOI: 10.1371/journal.pone.0034415 sha: 6b51562f63de5739f2b7ebf5f9c34365ac6ee545 doc_id: 807 cord_uid: fcffl6m4 Influenza viruses are among the most important human pathogens and are responsible for annual epidemics and sporadic, potentially devastating pandemics. (distantreader.org)
  • In this study, we describe a human monoclonal antibody (PN-SIA49) that recognizes a highly conserved epitope located on the stem region of the HA and able to neutralize a broad spectrum of influenza viruses belonging to different subtypes (H1, H2 and H5). (distantreader.org)
  • The 67A4 antibody recognizes human CD324 also known as E-cadherin, cadherin-1, and UVO. (biolegend.com)
  • The monoclonal antibody HEA125 which has been derived from immunisation of mice with a colon carcinoma cell line shows a broad reactivity pattern. (exalpha.com)
  • 4. Kemmner W., Moldenhauer G., Schlag P., and Brossmer R. (1992) Separation of tumor cells from a suspension of dissociated human colorectal carcionoma tissue by means of monoclonal antibody-coated magnetic beads. (exalpha.com)
  • Immunofluorescent localization of the reptilian Na + /H + exchanger protein using an antibody raised against a human NHE-1 fusion protein confirmed that protein expression paralleled abundant mRNA expression in epithelial cells of turtle stomach and colon, as well as in some nephron segments, and showed that the reptile NHE-1 homologue was localized exclusively to the basolateral membranes of these cells. (arizona.edu)
  • Tissue extracts of Mouse Testis (Lane 6) and Mouse Kidney (Lane 7) were electrophoresed using NuPAGE™ 3-8% Tris-Acetate Protein Gel (Product # EA0378BOX). (thermofisher.com)
  • ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, and unlike ZO-1, which is also expressed at cell junctions of cardiac myocytes, ZO-2 is not expressed in nonepithelial tissue. (thermofisher.com)
  • Cell culture or tissue culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. (wikipedia.org)
  • After the cells of interest have been isolated from living tissue , they can subsequently be maintained under carefully controlled conditions. (wikipedia.org)
  • In practice, the term "cell culture" now refers to the culturing of cells derived from multicellular eukaryotes , especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture , fungal culture, and microbiological culture (of microbes ). (wikipedia.org)
  • The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture . (wikipedia.org)
  • The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century. (wikipedia.org)
  • Since Haberlandt's original assertions, methods for tissue and cell culture have been realized, leading to significant discoveries in biology and medicine. (wikipedia.org)
  • The obvious place to start is with cells that match the animal and tissue that naturally host the virus. (the-scientist.com)
  • Conditional Met KO mice were generated using Cre‑loxP methodology and characterization of these mice indicated that the HGF‑Met signaling pathway is essential in regeneration, protection, and homeostasis in various tissue types and cells. (spandidos-publications.com)
  • With the electron microscopy (EM) team, Richard Fetter, Yalin Wang, and Mei Sun, our main discovery has been a new protocol for EM that will allow us to use CLEM for any fluorophore and fluorescent protein, by decreasing the auto-fluorescence of fixatives, the harsh conditions of OsO 4 , and increasing the ultra-structure preservation of cell tissue ( http://www.nature.com/nmeth/journal/v12/n3/full/nmeth.3225.html). (janelia.org)
  • The side effects consist mainly of bone marrow suppression and impaired central and peripheral neuronal tissue, gastrointestinal tissue, and kidney function. (biomedcentral.com)
  • 2010). Airborne vapor diffuses to the air:tissue interface and, if may contribute to the human-rat differences in diacetyl-induced soluble, enters the tissue phase. (cdc.gov)
  • If a virus turns out to be picky, several cell lines, such as HeLa, welcome a variety of viruses, Bloom says. (the-scientist.com)
  • Immortal cell lines such as HeLa are convenient, easy to grow, and highly reproducible, due to their clonal nature. (the-scientist.com)
  • ΔTolC showed a substantial decrease in the porcine aortic vascular endothelial cell (PAVEC) adherence, invasion, and pro-inflammatory response, in contrast to that of the wild type, with a reduced survival ratio in both the bacterial load and whole blood in mice. (bvsalud.org)
  • EMT, a highly conserved cellular program in many important phases of embryonic development, is a biological process through which epithelial cells lose their epithelial cobblestone phenotype and acquire fibroblastic and mesenchymal-like phenotypes. (biomedcentral.com)
  • 3. Simon B., Podolsky, D.K., Moldenhauer G., Isselbacher K.J., Gattoni-Celli, S., and Brand S.J. (1990) Epithelial glykoprotein is a member of a family of epithelial cell surface antigens homologous to nidogen, a matrix adhesion protein. (exalpha.com)
  • Human alveolar adenocarcinoma epithelial A549 cells were maintained in Ham's F12-K containing 10% FCS. (cdc.gov)
  • Extraintestinal pathogenic Escherichia coli (ExPEC) is a well-known critical pathogenic zoonosis that causes extraintestinal infections in humans and animals by affecting their immune organs. (bvsalud.org)
  • The HGF‑Met signaling pathway contributes in a paracrine manner to the development of epithelial organs, exerts regenerative effects on the epithelium, and promotes the regression of fibrosis in numerous organs. (spandidos-publications.com)
  • PMA + ionomycin - stimulated (6 hours) human peripheral blood lymphocytes (in the presence of monensin) were stained with CD3 FITC, fixed, permeabilized and intracellularly stained with CD3 FITC and IL-17A (clone BL168) APC (top) or mouse IgG1, κ APC isotype control (bottom). (biolegend.com)
  • Human colon carcinoma cell line HT-29 was stained with CD324 (clone 67A4) APC/Fire™ 750 (filled histogram) or mouse IgG1, κ APC/Fire™ 750 isotype control (open histogram). (biolegend.com)
  • We investigated the expression of receptor tyrosine kinase-like orphan receptor (ROR) 2 and Wnt5a and their prognostic significance in non-small cell lung cancer (NSCLC). (oncotarget.com)
  • 80% of lung cancer patients have non-small cell lung cancer (NSCLC) [ 1 - 3 ]. (oncotarget.com)
  • Using these methods, it could be established that the proliferating cells in Bowman's space, which are characteristically found in RPGN, are derived almost exclusively from the glomerular epithelium - primarily PECs. (scienceopen.com)
  • It is labelling most human epithelial cells and epithelium derived tumours. (exalpha.com)
  • 1. Moldenhauer G., Momburg F., Möller P., Schwartz, and Hämmerling G.J. (1987) Epithelium-specific surface glycoprotein of Mr 34,000 is a widely distributed human carcinoma marker. (exalpha.com)
  • We show here a novel molecular mechanism by which antiviral drugs of the nucleoside analog family could lead to impairments of the kidney, bone marrow, gastrointestinal, and neuronal functions, and how these side effects could possibly be restricted by the presence of TDP or uridine. (biomedcentral.com)
  • For example, work with mice lacking anion exchanger Slc26a6, which can function as an oxalate transporter, revealed a phenotype of calcium oxalate kidney stones. (yale.edu)
  • Often the desired cell types are available from commercial vendors: for example, 21 families of human primary cells are sold by Lonza , including hepatocytes ($500 for an ampule containing 3-6 million cells). (the-scientist.com)
  • HGF was cloned as a growth factor for hepatocytes ( 1 , 2 ), is identical to scatter factor (SF) and was originally discovered as a fibroblast-derived cell motility factor for epithelial cells ( 3 ). (spandidos-publications.com)
  • Viable CVA10 viruses were harvested by transfecting the viral mRNA into human rhabdomyosarcoma (RD) cells. (virosin.org)
  • This phase I trial assessed the safety, efficacy, and immunologic responses to minor histocompatibility antigens following nonmyeloablative allogeneic hematopoietic cell transplantation as treatment for metastatic renal cell carcinoma. (aacrjournals.org)
  • Treatment of metastatic renal cell carcinoma with allogeneic hematopoietic cell transplantation after nonmyeloablative conditioning with fludarabine/total body irradiation is feasible and may induce tumor regression or stabilization in some patients. (aacrjournals.org)
  • In addition to possess cross drug resistance characteristic, emerging evidences have shown that multiple-drug resistance (MDR) cancer cells exhibit aberrant metastatic capacity when compared to parental cells. (biomedcentral.com)
  • In this regard, elucidating the molecular mechanisms underlying aberrant metastatic capacity of MDR cells is quite important, as it may provide new targets for improving the efficiency of chemotherapy. (biomedcentral.com)
  • This process shares many similarities with epithelial-mesenchymal transition (EMT), which has been proposed as one of critical mechanisms for the acquisition of metastatic capacity by epithelial cancer cells [ 7 ]. (biomedcentral.com)
  • Patients were monitored for donor engraftment of myeloid and lymphoid cells, for clinical response by serial imaging, and for immunologic response by in vitro isolation of donor-derived CD8 + CTLs recognizing recipient minor histocompatibility (H) antigens. (aacrjournals.org)
  • CD8 + CTL clones recognizing minor H antigens were isolated from five patients studied. (aacrjournals.org)
  • Clones from three patients with a partial response or stable disease recognized antigens expressed on renal cell carcinoma tumor cells. (aacrjournals.org)
  • CD8 + CTL-recognizing minor H antigens on tumor cells can be isolated posttransplant and could contribute to the graft- versus -tumor effect. (aacrjournals.org)
  • In the kidney, urate is freely filtered at the glomerulus, reabsorbed, secreted, and then again reabsorbed in the proximal tubule. (medscape.com)
  • This gene expression is stimulated through various signaling factors in cells. (wikipedia.org)
  • ICC/IF: HEK-293T cells transfected with a human Uteroglobin expression vector containing a myc-his tag. (abcam.com)
  • Furthermore, we observed that JNK signaling may control the expression of both snail and twist1 in KB/VCR cells, indicating that both snail and twist1 are involved in controlling the mesenchymal characteristics of KB/VCR cells by JNK signaling. (biomedcentral.com)
  • Cell cultures have played a critical role in modern infectious disease research, particularly in the area of viruses and the expression of viral gene products," Bloom says. (the-scientist.com)
  • We also find that expression of CK1ε is able to promote oncogenic transformation of human cells in a β-catenin-dependent manner. (plos.org)
  • Expression cloning of an epithelial amiloride-sensitive Na+ channel. (wikidata.org)
  • The cell line RK13, which is derived from rabbit kidney epithelial cells and has little or no endogenous PrP expression, has proved to be an especially versatile tool. (cureffi.org)
  • dl 1520 replicated selectively in GC cells harboring an abnormal p53 pathway, including p53 mutation and the loss of p14 ARF expression, but did not in normal epithelial cells. (biomedcentral.com)
  • In cultured GC cells, dl 1520 rescued Ad-Endo replication, and dramatically promoted endostatin expression by Ad-Endo in a dose- and time-dependent manner. (biomedcentral.com)
  • The transgenic expression of Ad5 E1A and E1B19K simulated the rescue effect of dl 1520 supporting Ad-Endo replication in GC cells. (biomedcentral.com)
  • Description: Determined in a displacement NanoBRET assay using BODIPY-cyclopamine and nanoluciferase (Nluc)-tagged FZD 6 , in cells with CRISPR/Cas9 disabled SMO expression. (guidetopharmacology.org)
  • The relatively high level of NHE-1 expression in epithelial cells, particularly those of the colon and stomach, suggests that NHE-1 function is important for the maintenance or regulation of ion transport processes that occur in these cell types. (arizona.edu)
  • A comprehensive understanding of gene expression in human RB is essential for the development of safe and effective new therapies. (molvis.org)
  • The regulatory elements required for this cell-specific gene expression are likely located within its proximal promoter. (molvis.org)
  • The name of my M.S. project was "Cloning, expression, purification, and biochemical analysis of Var-1: a novel protein of Xenorhabdus nematophila" (paper in preparation). (janelia.org)
  • Significant downregulation of beta-defensin1 gene (DEFB1) expression was observed when human bronchial epithelial cells (HBEpCs) were exposed to IAV. (cdc.gov)
  • The encoded protein plays a role in cell-cycle progression and spindle organization, regulates cytokinesis, interacts with Nephrocystin 8 and is required for cilia formation. (nih.gov)
  • Gene therapy using a recombinant adenovirus (Ad) encoding secretory human endostatin (Ad-Endo) has been demonstrated to be a promising antiangiogenesis and antitumor strategy of in animal models and clinical trials. (biomedcentral.com)
  • Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin. (thermofisher.com)
  • Furthermore, we demonstrated the biological relevance of this mechanism of transcriptional initiation within the context of TGFB1 signaling and epithelial-mesenchymal transition (EMT). (nature.com)
  • In this study, we explored the contribution of c-Jun N-terminal kinases (JNK) signaling to the mesenchymal phenotypes and the aberrant motile capacity of MDR cells utilizing a well characterized MDR cell line KB/VCR, which is established from KB human epidermoid carcinoma cells by vincristine (VCR), and its parental cell line KB. (biomedcentral.com)
  • Taking advantage of experimental strategies including pharmacological tool and gene knockdown, we showed here that interference with JNK signaling pathway by targeting JNK1/2 or c-Jun reversed the mesenchymal properties of KB/VCR cells to epithelial phenotypes and suppressed the motile capacity of KB/VCR cells, such as migration and invasion. (biomedcentral.com)
  • These observations support a critical role of JNK signaling in maintaining the mesenchymal properties of KB/VCR cells. (biomedcentral.com)
  • and JNK signaling may maintain the mesenchymal characteristics of KB/VCR cells potentially through snail and twist1. (biomedcentral.com)
  • Prediction of the coding sequences of unidentified human genes. (wikipedia.org)
  • Conserved transcription factor-binding sites within promoter regions of human RON genes were then searched. (spandidos-publications.com)
  • Linkage and comparative mapping studies indicate that the transferred chromosome segment contains a number of candidate genes for hypertension, including genes encoding a brain dopamine receptor and a renal epithelial potassium channel. (jci.org)
  • Despite significant advances in understanding nephron segment patterning, many questions remain about the underlying genes and signaling pathways that orchestrate renal progenitor cell fate choices and regulate differentiation. (mdpi.com)
  • However, during kidney failure, the intestinal contribution of urate excretion increases to compensate for the decreased elimination by the kidneys. (medscape.com)
  • Intestinal excretion of urate is handled by uric acid transporters in intestinal epithelial cells, which transport uric acid from the blood to the intestinal lumen. (medscape.com)
  • Protein transport protein Sec16B also known as regucalcin gene promoter region-related protein p117 (RGPR-p117) and leucine zipper transcription regulator 2 (LZTR2) is a protein that in humans is encoded by the SEC16B gene. (wikipedia.org)
  • The gene consists of 26 exons spanning approximately 4.1 kbp and is localized on human chromosome 1q25.2. (wikipedia.org)
  • Human IL-17A shows 63%, 63%, and 72% amino acid sequence identity to rat IL-17A, mouse IL-17A, and a protein encoded by the ORF13 gene of herpesvirus Saimiri (HVS), respectively. (biolegend.com)
  • A causative gene for this disease, NPHS2, was mapped to 1q25-31 and we report here its identification by positional cloning. (scienceopen.com)
  • TIM-2 is an ~55 kDa type I transmembrane glycoprotein that is encoded by Timd2 (T cell immunoglobulin and mucin domain containing 2) which belongs to the immunoglobulin gene superfamily. (bdbiosciences.com)
  • We also demonstrated that the −657 to +411 DCT promoter fragment efficiently directs RB cell-specific transcription of the luciferase reporter gene in cell lines. (molvis.org)
  • The enzyme flavohemoglobin, coded by the hmp gene, is involved in protecting bacterial cells from the toxic effects of NO and represents a potentially interesting target for development of novel treatment concepts against resistant uropathogenic bacteria. (biomedcentral.com)
  • pendent of those associated with cretory ducts, columnar epithelia the KRAS gene is more frequent- other key mechanistic characteris- lining the gastrointestinal tract and ly mutated in human cancer, which tics of IARC Group 1 carcinogens. (who.int)
  • Molecular Biology of the Cell. (wikipedia.org)
  • CD324, a member of the cadherin superfamily, is a calcium-dependent, transmembrane cell-cell adhesion glycoprotein composed of 4 extracellular cadherin repeats and a highly conserved cytoplasmic tail region with a predicted molecular weight of approximately 100 kD. (biolegend.com)
  • Consequently, whether the SARS-CoV has "jumped" from a nonhuman host reservoir to humans and the molecular basis of such a jump remain unanswered questions ( 12 ). (cdc.gov)
  • Camel cells would be a first choice for Middle East Respiratory Syndrome coronavirus (MERS-CoV), for example. (the-scientist.com)
  • The participation of activated PECs in both diseases identifies these cells as prime pharmacological targets to develop more specific therapies for both diseases. (scienceopen.com)
  • To gain insight into SARS-CoV biopathology, we analyzed the relevant features of SARS-CoV HSR1 growth in vitro, including the ultrastructural analysis of the consequences of virus replication in Vero cells. (cdc.gov)
  • Human beings have higher levels of uric acid, in part, because of a deficiency of the hepatic enzyme uricase, and a lower fractional excretion of uric acid. (medscape.com)
  • Normally, two thirds of uric acid excretion occurs through the kidneys and one third through the intestines. (medscape.com)
  • Our general goal is to characterize the mechanisms regulating sodium, acid-base, and anion excretion by the kidney. (yale.edu)
  • Growing viruses in cell cultures allowed preparation of purified viruses for the manufacture of vaccines . (wikipedia.org)
  • This vaccine was made possible by the cell culture research of John Franklin Enders , Thomas Huckle Weller , and Frederick Chapman Robbins , who were awarded a Nobel Prize for their discovery of a method of growing the virus in monkey kidney cell cultures. (wikipedia.org)
  • That's why virologists need cell cultures, but to wield those cultures well they must understand both viruses and host cells. (the-scientist.com)
  • The instruments that I was using were 4 confocal systems (one with 2-photon incorporated), and 3 wide-field systems, for either live-cell imaging or fixed specimens, using different cell cultures. (janelia.org)
  • newborn and weaning mice, primary chick embryo cell cultures and C6/36 clone of Aedes albopictus cells. (cdc.gov)
  • Genetic mutagenesis analysis revealed that nhaA deletion resulted in filamentous bacterial morphology and rendered the bacteria more susceptible to sodium dodecyl sulfate, suggesting the role of nhaA in maintaining cell envelope integrity. (bvsalud.org)
  • To uncover genetic dependencies in breast cancer cells that harbor active β-catenin signaling, we performed RNAi-based loss-of-function screens in breast cancer cell lines in which we had characterized β-catenin activity. (plos.org)
  • and they play roles in human genetic diseases, with functional disorders causing diseases. (tcdb.org)
  • In summary, reverse genetic tools for prototype strain of CVA10, including both the infectious clone and the SRIPs system, were successfully established. (virosin.org)
  • 293T cells and chicken fibroblast DF-1 cells were maintained in Dulbecco's modified Eagle's medium (DMEM) containing 10% fetal calf serum (FCS). (cdc.gov)
  • H9 cells were grown in suspension in RPMI-1640 medium supplemented with 10% fetal calf serum, 2 mM glutamine, 100 IU/ml penicillin, 100 µ g/ml streptomycin at 37°C, 5% CO 2 , 95% air. (biomedcentral.com)
  • Renal cell carcinoma (RCC) is one of the ten most common cancers for men and women with an approximate 75% overall 5-year survival. (frontiersin.org)
  • Sixteen histological tumor subtypes exist and the most common are papillary, chromophobe and clear cell renal cell carcinoma (ccRCC) representing 85% of all RCC. (frontiersin.org)
  • In bacterial pathogenesis, the ectodomain of CD324 mediates bacterial adhesion to mammalian cells, while the cytoplasmic domain is required for internalization. (biolegend.com)
  • For metastasis from a primary tumor site, cancer cells must lose cell-cell adhesion and acquire motility to invade adjacent cell layers [ 6 ]. (biomedcentral.com)
  • Specifically, ERV-K members were originally identified due to their similarity to the Mouse Mammary Tumor Virus (MMTV) and are subdivided into ten so-called human MMTV-like clades (HML1-10) ( 2 , 3 ). (frontiersin.org)
  • Eventually part of my work was published in a paper called "Mitochondrial targeting for photochemotherapy: Can selective tumor cell killing be predicted based on n-octanol/water distribution coefficients? (janelia.org)
  • The luciferase activities in the A549 cells incubated at 33 or 37°C and the DF-1 cells incubated at 39°C were measured by using the Dual-Glo Luciferase Assay System (Promega) at 24 h post-transfection. (cdc.gov)
  • We examined the activities of cloned promoter DNA fragments with luciferase assay. (molvis.org)
  • Part of cellular calcium is sequestered in intracellular organelles, including endoplasmic reticulum, sarcoplasmic reticulum (SR) in muscle cells and in mitochondria. (abdominalkey.com)
  • Plasmalemmal Cl - -HCO3 - exchangers regulate intracellular pH and [Cl - ] and cell volume. (tcdb.org)
  • Specific point mutations in human anion exchanger 1 (AE1) convert this electroneutral anion exchanger into a monovalent cation conductance. (tcdb.org)
  • NADPH oxidase 4 (NOX4), a source of cellular superoxide anion, has multiple biological functions that may be of importance in inflammation, and in the pathogenesis of human metabolic diseases, including diabetes. (docksci.com)
  • Human SLC4A1/AE1 mutations cause either the erythroid disorders spherocytic haemolytic anaemia or ovalocytosis, or distal renal tubular acidosis. (tcdb.org)
  • Activating mutations upstream may also underlie some epigenetic or within the ERK1/2 cascade are events that change cell signalling. (who.int)
  • Structural requirements and sequence motifs for polarized sorting and endocytosis of LDL and Fc receptors in MDCK cells. (wikidata.org)
  • Furthermore, we demonstrate that breast cancer cells that harbor activated β-catenin activity exhibit enhanced sensitivity to pharmacological blockade of Wnt/β-catenin signaling. (plos.org)
  • Basolateral membranes of turtle (Pseudemys scripta) colon epithelial cells exhibit robust Na + /H + exchange activity that can be activated by cell shrinkage and is blocked by amiloride [M. A. Post and D. C. Dawson. (arizona.edu)