Human induced pluripotent stem functional cardiomyocyte cardiomyocytes. Medical search. Frequent questions

Cardiomyocytes (CMs) derived from human pluripotent stem cells (hPSCs) provide a valuable cell source for regenerative therapy, disease modeling, and drug screening. (biomedcentral.com)
Functional CMs derived from human pluripotent stem cells (hPSCs) would provide an unprecedented cell source for disease modeling, drug discovery, and cell transplantation therapy for CVD patients [ 3 ]. (biomedcentral.com)
Functional cardiomyocytes can be efficiently derived from human pluripotent stem cells, which collectively include embryonic and induced pluripotent stem cells (iPSC). (re-place.be)

Human engineered cardiac tissues (hECTs) are derived by experimental manipulation of pluripotent stem cells, such as human embryonic stem cells (hESCs) and, more recently, human induced pluripotent stem cells (hiPSCs) to differentiate into human cardiomyocytes. (wikipedia.org)
By employing microRNA expression profiling and functional knockdown studies on human embryonic stem cell-derived cardiomyocytes, the authors identified miR-363 as an upstream negative regulator of left ventricular specification transcription factor HAND1 . (biomedcentral.com)
miRNAs could thus be the best targets for understanding cardiac specialization during differentiation of human embryonic stem cells (hESCs). (biomedcentral.com)
Human induced pluripotent stem cells (hiPSCs) present an exciting alternative for modeling early human embryogenesis due to their well-established ability to differentiate into all embryonic germ layers. (stanford.edu)

Researchers at Michigan Tech and Harvard Medical School have developed a new way to create cardiomyocytes from induced pluripotent stem cells (iPSCs), for improved cardiac cell therapy. (medgadget.com)
These lab-fabricated, fluorescently stained cardiomyocytes-heart muscle cells-exhibit the maturation and functionality of heart cells grown within a heart. (medgadget.com)
However, creating fully functional cardiac cells can be a challenge. (medgadget.com)
Unfortunately stem cell therapeutics don't have high success rates partly because the cells are not mature and fully functional. (medgadget.com)
Programming induced pluripotent stem cells into heart muscle cells requires biochemical and biomechanical cues. (medgadget.com)
This results in more robust, mature and functional cardiomyocyte-like cells. (medgadget.com)
As the cells mature, they begin to beat, and more closely resemble mature, native cardiomyocytes. (medgadget.com)
In pathological conditions, damaged cells transfer dysfunctional mitochondria toward recipient cells to ask for help and take up exogenous functional mitochondria to alleviate injury. (frontiersin.org)
They demonstrated that transferring functional mitochondria of bone marrow-derived stem cells to defective parenchymal cells increases the aerobic respiration capacity of recipient mitochondria ( 7 ). (frontiersin.org)
Nowadays, more and more studies have revealed that cells in the cardiovascular system (such as cardiomyocytes, vascular smooth muscle cells, endothelial cells, et al. (frontiersin.org)
We validate optical actuation by virally introducing optogenetic drivers in rat and human cardiomyocytes or through the modular use of dedicated light-sensitive somatic 'spark' cells. (nature.com)
Human pluripotent stem cells are differentiated into cardiomyocytes (hPSC-CMs) in culture through a milieu containing small-molecule mediators (e.g. cytokines, growth and transcription factors). (wikipedia.org)
Amend N, Thiermann H, Worek F, Wille T ( 2019 ) The arrhythmogenic potential of nerve agents and a cardiac safety profile of antidotes - A proof-of-concept study using human induced pluripotent stem cells derived cardiomyocytes (hiPSC-CM). Toxicol Lett 308:1-6. (multichannelsystems.com)
The discovery of human-induced pluripotent stem cells (iPSCs) is highly expected to provide the breakthrough to this obstruction. (elsevierpure.com)
This first-in-human clinical trial aimed to confirm the safety of cardiomyocyte patch transplantation derived from allogeneic induced pluripotent stem (iPS) cells based on the results of several preclinical studies. (bvsalud.org)
The efficient derivation of specialized and functional cardiomyocytes (CMs) from pluripotent stem cells is a primary goal for stem cell-based cardiac regenerative therapies. (biomedcentral.com)
Furthermore, we conduct a cross-species prediction of cardiomyocyte subtypes from in vitro-derived human induced pluripotent stem cells and unexpectedly uncover a predominance of left ventricular (LV) identity that we confirmed by an LV-specific TBX5 lineage tracing system. (stanford.edu)
Modulation of dynamics in a pre-existing hippocampal network by neural stem cells on a microelectrode array, J. Neural Eng. (u-tokyo.ac.jp)
We are now able to fabricate cell sheets using cardiomyocytes and endothelial cells differentiated from human induced Pluripotent Stem (iPS) cells and are aiming to use our technology to create human 3D tissue with cardiac functionality. (heraldopenaccess.us)
The integration of in vitro cardiac tissue models, human induced pluripotent stem cells (hiPSCs) and genome-editing tools allows for the enhanced interrogation of physiological phenotypes and recapitulation of disease pathologies. (nature.com)
Stem Cells Transl. (nature.com)
Recent advances in developmental and stem cell biology have paved the way for a wide spectrum of novel cardiovascular therapies, from the transplantation of stem cell-derived cells to the stimulation of endogenous regeneration processes. (tum.de)
In the research projects outlined below, we combine 2D and 3D in vitro models based on human pluripotent stem cells with animal models such as the mouse and pig. (tum.de)
Human induced pluripotent stem cells (hiPSCs) are stem cells that are derived from the somatic cells (e.g. blood or skin cells) of healthy individuals or patients. (tum.de)
We routinely produce engineered heart tissue based on hiPSC-derived cardiac cells seeded on decellularized ventricular matrix from pigs or non-human primates, which we cultivate in a biomimetic chamber system promoting tissue maturation by continuous mechanical and electrical stimulation. (tum.de)
We have also established an ex vivo embryo culture system that allows us to study the function of human cardiac cells in human-mouse or human-pig chimeras. (tum.de)
You can record from neuronal or cardiac cultures, stem cells, or brain or cardiac slices. (multichannelsystems.com)

Kraushaar U, Guenther E ( 2019 ) Assay Procedures for Compound Testing of hiPSC-Derived Cardiomyocytes Using Multiwell Microelectrode Arrays. (multichannelsystems.com)
In order to develop the prototype HeartCHIP II system, the consortium partners will first study the constituent parts of the planned method: 1) an organ-on-chip (OOC) culture method for human induced pluripotent stem cell (hiPSC) derived multi-cell (cardiomyocyte, endothelial and fibroblast) 3D cultures, 2) dedicated software for functional video microscopy analysis of read-out parameters and 3) disease modelling using hiPSC-derived cardiomyocytes with disease-specific mutations. (erasmusmc-rdo.nl)
Confocal immunofluorescence image of hiPSC-derived cardiomyocytes stained for the sarcomere proteins cardiac troponin T (red) and a -actinin (green). (tum.de)

Current methods for the maturation of induced pluripotent stem cell-derived cardiomyocytes. (wjgnet.com)
Introduction: Understanding the critical factors for the maturation of human induced pluripotent stem cell (hiPSC)-derived cardiac tissue is important for further development of culture techniques. (bvsalud.org)

This 3D scaffold, along with collagen - a major component of the cardiac extracellular matrix - provides the appropriate conditions to promote cardiomyocyte organization, growth and differentiation. (wikipedia.org)
MicroRNAs regulate target gene expression post-transcriptionally in a myriad of cell types and play critical roles in diverse physiological and pathological processes, including cardiomyocyte development, differentiation, and regeneration. (biomedcentral.com)
This commentary discusses the findings from Wagh and colleagues published in this issue of Stem Cell Research and Therapy demonstrating a critical role for miR-363 in post-transcriptional regulation of CM differentiation via the hand and neural crest derivative expressed HAND1 transcription factor [ 1 ]. (biomedcentral.com)
Identifying molecular regulators and critical mediators of cardiac cell type development, proliferation, and differentiation is of great clinical importance, and unraveling such molecular horizons could lead to the development of therapeutic strategies for successful regeneration of the human adult heart. (biomedcentral.com)
Combined lineage tracing and scRNA-seq reveals unexpected first heart field predominance of human iPSC differentiation. (stanford.edu)

Ma S, Ma J, Tu Q, Zheng C, Chen Q, Lv W ( 2020 ) Isoproterenol Increases Left Atrial Fibrosis and Susceptibility to Atrial Fibrillation by Inducing Atrial Ischemic Infarction in Rats. (multichannelsystems.com)

however, the distribution and organization of many of these structures is characteristic of neonatal heart tissue rather than adult human heart muscle. (wikipedia.org)
Tahrir FG, Gordon J, Feldman AM, Cheung J, Khalili K, Mohseni Ahooyi T ( 2019 ) Evidence for the impact of BAG3 on electrophysiological activity of primary culture of neonatal cardiomyocytes. (multichannelsystems.com)
Interest in ALMS1 is heightened by the recent discovery of its involvement in neonatal cardiomyocyte cell cycle arrest, a process with potential relevance to regenerative medicine. (springer.com)

In this study, we demonstrated an approach using human induced pluripotent stem cell‐derived atrial and ventricular cardiomyocytes (hiPSC‐aCMs and hiPSC‐vCMs, respectively) coupled with a sophisticated optical mapping system for drug screening of atrial‐selective compounds in vitro. (sfu.ca)
Oleaga C, Jalilvand G, Legters G, Martin C, Ekman G, McAleer CW, Long CJ, Hickman JJ ( 2019 ) A human in vitro platform for the evaluation of pharmacology strategies in cardiac ischemia. (multichannelsystems.com)
They have the capacity to self-renew indefinitely and to give rise to any cell type of the body in vitro , making them an exceptionally powerful tool for studying human disease in patient-specific backgrounds. (tum.de)

Fluorescent stem cell reporters provide a convenient and efficient method to purify expected CM subtypes. (biomedcentral.com)

Sun J, Wugeti N, Mahemuti A ( 2019 ) Reversal effect of Zhigancao decoction on myocardial fibrosis in a rapid pacing-induced atrial fibrillation model in New Zealand rabbits. (multichannelsystems.com)

Functional characterization using optical mapping showed that hiPSC‐aCMs have shorter action potential durations and faster Ca2+ handling dynamics compared with hiPSC‐vCMs. (sfu.ca)

However, due to the inaccessibility of human heart tissue, we couldn't obtain the sufficient amount of patient's heart tissues. (elsevierpure.com)
While these populations have been extensively studied in non-human model systems, their identification and study in vivo human tissues have been limited due to the ethical and technical limitations of accessing gastrulation stage human embryos. (stanford.edu)
Here, we describe a novel strategy that overcomes the limitation of passive diffusion by introducing functional capillaries into artificial tissues. (heraldopenaccess.us)
The development of a novel method of introducing functional vascular networks into artificial tissues will facilitate the creation of thick 3D tissues/organs that are cell-dense and vascular-rich (e.g., cardiac, hepaticorrenal tissue). (heraldopenaccess.us)
Feric, N. T. & Radisic, M. Maturing human pluripotent stem cell-derived cardiomyocytes in human engineered cardiac tissues. (nature.com)

iPSCs show significant potential for cardiac regenerative medicine, as researchers can use biochemical cues to "program" them into a cardiomyocyte-like cell, which shows regenerative efficacy in damaged cardiac muscle. (medgadget.com)

Here, we describe the development of a TBX5/MYL2 lineage tracing reporter system that allows for the identification of FHF- progenitors and their descendants including left ventricular cardiomyocytes. (stanford.edu)
We compared this data with existing murine and 3D cardiac organoid scRNA-seq data and confirmed the dominance of left ventricular cardiomyocytes (>90%) in our hiPSC-derived progeny. (stanford.edu)

Multi-omics technology can elucidate disease mechanisms in ICI-induced myocarditis. (stanford.edu)
Mechanisms regulating cardiomyocyte cell cycle arrest are of great interest partly because reversing this process could provide a way to stimulate cardiac regeneration after injury [ 17 ]. (springer.com)

In this review, we summarized the mechanism of mitochondrial transfer in the cardiovascular system and outlined the fate and functional role of donor mitochondria. (frontiersin.org)
Cardiovascular diseases (CVD) claim millions of lives per year and pose a severe threat to economics and human health. (biomedcentral.com)
hECTs provide a valuable resource to reproduce the normal development of human heart tissue, understand the development of human cardiovascular disease (CVD), and may lead to engineered tissue-based therapies for CVD patients. (wikipedia.org)

The team developed a culture mold that mimics the physiological conditions under which cardiomyocytes grow. (medgadget.com)

Strande, J. L. Haploinsufficiency MYBPC3 mutations: another stress induced cardiomyopathy? (nature.com)

The improvement of preclinical cardiotoxicity testing, discovery of new ion-channel-targeted drugs, and phenotyping and use of stem cell-derived cardiomyocytes and other biologics all necessitate high-throughput (HT), cellular-level electrophysiological interrogation tools. (nature.com)

Therefore, an important goal of our recent research has been to increase the thickness and functionality of bioengineered myocardial tissue through the addition of a functional vascular network. (heraldopenaccess.us)
The system will uniquely use functional video microscopy of heart tissue cultures as a read-out parameter. (erasmusmc-rdo.nl)

NIEHS research uses state-of-the-art science and technology to investigate the interplay between environmental exposures, human biology, genetics, and common diseases to help prevent disease and improve human health. (nih.gov)
Herein we introduce the outline of creation for cardiac disease modeling using patient-specific iPSCs.Within several topics, we present the actual representative methodologies throughout the process from the derivation of cardiomyocytes to those of functional analysis. (elsevierpure.com)

Current drug development efforts for the treatment of atrial fibrillation are hampered by the fact that many preclinical models have been unsuccessful in reproducing human cardiac physiology and its response to medications. (sfu.ca)

and a 1-year observation period that included a 3-month immunosuppressive drug administration period after transplantation of iPS cell-derived cardiomyocyte patches to evaluate adverse events, cardiac function, myocardial blood flow, heart failure symptoms, and immune response. (bvsalud.org)

Live immunofluorescence imaging of a 3D cardiac spheroid derived from a double reporter stem cell line expressing eGFP and mCherry in the endogenous loci of the cardiac transcription factors NKX2.5 and TBX5 , respectively. (tum.de)

Currently required preclinical cardiotoxicity testing (part of the drug development process, Fig. 1 ) specifically focuses on a drug's blocking action on the hERG K + channel, which provides one of the main repolarizing currents in cardiomyocytes. (nature.com)

The recent publication in Stem Cell Research and Therapy by Wagh and colleagues reports a novel regulatory role for miR-363 in cardiomyocyte specification. (biomedcentral.com)

In our research group, this cardiomyocyte platform presents opportunities 1. (re-place.be)

Kenta Shimba, Kiyoshi Kotani, Yasuhiko Jimbo, Microfabricated Device to Record Axonal Conduction under Pharmacological Treatment for Functional Evaluation of Axon Ion Channel, IEEE Transactions on Biomedical Engineering, Vol. 68, No. 12, 3574-3581, 2021. (u-tokyo.ac.jp)

The National Institute of Environmental Health Sciences (NIEHS) is expanding and accelerating its contributions to scientific knowledge of human health and the environment, and to the health and well-being of people everywhere. (nih.gov)

Some studies have suggested a role for this protein in maintaining centriole-nucleated sensory organelles termed primary cilia, and AS is now considered to belong to the growing class of human genetic disorders linked to ciliary dysfunction (ciliopathies). (springer.com)

Insufficient or inadequate tools for predicting failure before more expensive phases of testing both in animal and human, drive up the drug costs and decrease the desire for pharmaceutical companies to pursue more 'high-risk' drugs that would result in little payout. (nature.com)
In response, international regulatory agreements were developed that mandate testing of all new drugs, both cardiac and non-cardiac, for cardiac liability, including drug-induced long QT interval (LQT) and risk for development of life-threatening arrhythmias, such as Torsade de Pointes (TdP) 5 . (nature.com)
Human iPSC-based cardiac microphysiological system for drug screening applications. (nature.com)

Through the patient-specific iPSCs-derived cardiomyocytes, we could analyze the patient-specific heart diseases directly and repetitively. (elsevierpure.com)

Anatomy51

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