TransplantationStellate cellsHepaticHSCsProliferationVivoProgenitorAllogeneicDonorDamaged tissueBiologyHaematopoietic stem cellsAssaysSubsetsImmune cellsMesenchymalPluripotent stemVitroGVHDSquamous Cell CarcAdultReceptorsReconstitutionLineagesGene expressionInflammationImmunodeficiency virusHematopoiesisRegenerative MedicineChimeric antigen receptorEngraftment of human cellsRegulatesMiceClinicalMetabolismComplicationsMurineRegenerationDifferentiateNichesDifferentiation of humanGenesStromal cellsDemonstrateEmbryonicFlow CytometryEnrichmentTissuesGenerateEndothelial cellsOxidative stressTherapeuticHSPCsFindingsLiverFunctionalAcuteBone marrow derived cellsTranscriptomic SignatureDistinctEpithelial
Transplantation33
- Impact Our proposal will optimize the generation and viability of an unlimited, reproducible source of human engineered islets for transplantation. (ca.gov)
- Research Objective Development of immune tolerant human islet-like organoids for transplantation into diabetic patients. (ca.gov)
- Cell therapy (also called cellular therapy, cell transplantation, or cytotherapy) is a therapy in which viable cells are injected, grafted or implanted into a patient in order to effectuate a medicinal effect, for example, by transplanting T-cells capable of fighting cancer cells via cell-mediated immunity in the course of immunotherapy, or grafting stem cells to regenerate diseased tissues. (wikipedia.org)
- Although such attempts produced no positive benefit, further research found in the mid twentieth century that human cells could be used to help prevent the human body rejecting transplanted organs, leading in time to successful bone marrow transplantation as has become common practice in treatment for patients that have compromised bone marrow after disease, infection, radiation or chemotherapy. (wikipedia.org)
- In recent decades, however, stem cell and cell transplantation has gained significant interest by researchers as a potential new therapeutic strategy for a wide range of diseases, in particular for degenerative and immunogenic pathologies. (wikipedia.org)
- in 1968, in Minnesota, the first successful human bone marrow transplantation took place. (wikipedia.org)
- Bone marrow transplants are the most common and well established cell transplantation therapies. (wikipedia.org)
- For a long time, bone marrow transplantation was the only clinically applicable method of cell transplantation, however, since the 1990s, cell therapy has been investigated for a wide scale of pathologies and disorders. (wikipedia.org)
- For example, because hematopoietic stem cells can reconstitute the entire blood system, bone marrow transplantation has long been used in the clinic to treat various diseases. (articlecity.com)
- Similarly, the transplantation of other tissue-specific stem cells, such as stem cells isolated from epithelial and neural tissues, can treat mouse disease models and human patients in which epithelial and neural cells are damaged. (articlecity.com)
- The goal of our grant is to optimize the strategy of in utero transplantation of hematopoietic stem cells, with the ultimate goal of treating fetuses with congenital stem cell disorders. (ca.gov)
- Our project includes transplantation of HSC into both mice and non-human primates. (ca.gov)
- This year, we have continued our work with in utero transplantation of human HSCs into the fetuses of an immunodeficient mouse strain. (ca.gov)
- Hematopoietic stem cell (HSC) replacement transplantation can ameliorate diseased hematologic system, thus inhibiting immune disorders including autoimmune diseases and immunodeficient disorders. (stanford.edu)
- The resultant chimeric immune systems were demonstrated to be immunologically tolerant to heart tissue from the HSC donor, providing a safe platform for HSC transplantation as a means to solid organ transplantation. (stanford.edu)
- In experimental settings, the ability of human HSCs to reconstitute hematopoiesis is measured by transplantation into genetically immunocompromised mice (e.g. (stemcell.com)
- Other uses of the CFU assay include studying the effects of stimulatory and inhibitory growth factors, screening novel compounds to predict potential toxicity to the hematopoietic system, and testing the effects of various in vitro manipulations (e.g. cell processing, cryopreservation, gene transduction, and transmission) on cellular products used in hematopoietic cell transplantation. (stemcell.com)
- Recent advances in the understanding of immunological mechanisms of GVHD have shown that activation of donor immune cells is tightly regulated by the microenvironment tissue or digestive microbiota changes after transplantation. (pasteur.fr)
- The in vivo hematopoietic reconstitution potential of c-kit + cells derived from the mouse PSCs was evaluated via m-NSG transplantation assay. (biomedcentral.com)
- Currently, allogeneic HSC transplantation has been widely used in a clinical setting, yet allogeneic transplantation often leads to graft versus host disease (GVHD) [ 1 ]. (biomedcentral.com)
- The ability to grow blood stem cells (HSCs) in culture would greatly improve the patient outcome in transplantation, and make cord blood to be useful for adult applications. (gabriellesangels.org)
- We will further investigate why our cultured cells greatly enhance allogeneic transplantation, and identify the responsible cell populations facilitating the allograft. (gabriellesangels.org)
- HSC transplantation is the reference treatment of severe, non-malignant and malignant blood diseases. (univ-mrs.fr)
- GvHD and immune deficiency inherent to the kinetics of reconstitution are two major immunological complications of HSC transplantation, and represent the main cause of mortality and post-transplant morbidity. (univ-mrs.fr)
- Currently there is no way to efficiently predict the occurence of GvHD after HSC transplantation. (univ-mrs.fr)
- However, sustained engraftment of the expanded cells was not observed after transplantation, suggesting ineffective expansion of hematopoietic stem cells with long-term repopulating activity (LTR-HSCs). (confex.com)
- At optimal coating densities of Delta1, the frequency of LTR-HSCs measured by limiting dilution analysis 16 weeks after transplantation into NSG mice was 4.9- and 4.2-fold higher in hypoxic cultures (1 in 1,586 CD34+ cells) compared with uncultured cells (1 in 7,706) and the normoxia group (1 in 5,090), respectively. (confex.com)
- Conversely, we observed no difference in expression of the homing CXCR4 receptor between cells cultured under normoxic and hypoxic conditions, indicating that hypoxia increased the absolute numbers of LTR-HSCs but not their homing potential after transplantation. (confex.com)
- These strains show not only a high rate of human cell engraftment, but also generate well-differentiated multilineage human hematopoietic cells after human hematopoietic stem cell (HSC) transplantation. (nih.gov)
- Enforced expression of HOXB4 dramatically increases the regeneration of murine HSC following transplantation, and enhances the repopulating ability of human SCID repopulating cells. (dissertations.se)
- Collectively, these findings indicate that correct expression levels of HOXB4 improve proliferative recruitment of HSC in settings demanding high proliferation, for example after bone marrow transplantation. (dissertations.se)
- Sometimes stem cell transplantation is curative. (msdmanuals.com)
- Hematopoietic stem cell transplantation (HSCT) affects serious risks for the patient, including death. (bvsalud.org)
Stellate cells6
- Kupffer cells, hepatic stellate cells and sinusoidal endothelium) and infiltrating leukocytes (e.g., monocytes, monocyte-derived macrophages, neutrophils and lymphocytes). (mdpi.com)
- These researchers are working with human hepatic stellate cells (HSCs). (umn.edu)
- In the present study, we aimed to investigate the molecular mechanisms and prophylactic effects of grape seed proanthocyanidin (GSP) on lipopolysaccharide (LPS)-stimulated human hepatic stellate cells (HSCs). (spandidos-publications.com)
- However, there have been no studies on the anti-inflammatory effects of GSP in human hepatic stellate cells (HSCs). (spandidos-publications.com)
- CB2: Cannabis receptor 2, CBD: Cannabidiol, DNA: Deoxyribonucleic acid, HBV: Hepatitis B virus, HCV: Hepatitis C virus, HIV/AIDS: Human immunodeficiency virus/acquired immune deficiency syndrome, HSC: Hepatic stellate cells, MTS: 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2Htetrazolium, PCR: Polymerase chain reaction. (nih.gov)
- Rho-kinase (ROCK) activation in hepatic stellate cells (HSC) is a key mechanism promoting liver fibrosis and portal hypertension (PTH). (ibecbarcelona.eu)
Hepatic1
- Thus, LX-2 cells are considered a novel tool for analyzing hepatic fibrosis ( 12 ). (spandidos-publications.com)
HSCs31
- Bone marrow hematopoietic stem cells (HSCs) are vital for the maintenance of hematopoiesis. (irbbarcelona.org)
- Our recent work shows the existence and relevance of dormancy in human HSCs and identifies the hyaluronic acid-GPRC5C signalling axis as an essential component controlling the HSC state (Zhang et al. (irbbarcelona.org)
- Due to the scarcity of HSCs, technical challenges have limited their insights into the interplay between HSC metabolism and their transcriptional and epigenetic regulation. (irbbarcelona.org)
- HSCs make up a very small population of the hematopoietic system, however, these cells are invaluable as they have the potential to give rise to all mature blood and immune cell types and sustain life-long blood production. (stemcell.com)
- CD34 is the most commonly used cell surface marker to identify human HSPCs as it is expressed on HSCs, in addition to both multipotent and more differentiated progenitor cells of individual blood cell lineages. (stemcell.com)
- The group are currently focused on the pivotal role that HOXA cluster genes play in the demarcation and possible emergence of haematopoietic stem cells (HSCs) and their role in cancer. (nottingham.ac.uk)
- However, eradication of the leukaemia repopulating cell or stem cell whilst retaining normal HSCs remains a challenge with current experimental therapies. (nottingham.ac.uk)
- For example, HSC activation and the subsequent matrix secretion by activated HSCs induce liver fibrosis, leading to cirrhosis in chronic liver injury ( 10 ). (spandidos-publications.com)
- The reciprocal relationship between HSCs and precancerous hepatocytes or hepatoma cells promotes tumorigenesis, migration and invasion of cancer cells, and formation of metastasis ( 11 ). (spandidos-publications.com)
- The human HSC cell line, LX-2, exhibits the typical characteristics of HSCs under primary culture. (spandidos-publications.com)
- Hematopoietic stem cells (HSCs) emerge from hemogenic endothelial (HE) cells, through endothelial-to-hematopoietic transition (EHT). (lu.se)
- However, metabolic processes underlying the emergence of HSCs from HE cells remain unclear. (lu.se)
- Decades of efforts have attempted to differentiate the pluripotent stem cells (PSCs) into truly functional hematopoietic stem cells (HSCs), yet the problems of low differentiation efficiency in vitro and poor hematopoiesis reconstitution in vivo still exist, mainly attributing to the lack of solid, reproduced, or pursued differentiation system. (biomedcentral.com)
- We have identified several new growth factors, and established a very potent cell culture system for growing human HSCs. (gabriellesangels.org)
- These severely immunodeficient animals are required to support human cell engraftment to generate multiple differentiated lineages from human hematopoietic stem cells (HSCs). (genengnews.com)
- A broadly used technique for humanizing mice, termed Hu-HSC, involves the transfer of HSCs isolated from a variety of sources. (genengnews.com)
- Long-term hematopoietic stem cells (LT-HSCs) maintain hematopoietic output throughout an animal's lifespan. (biorxiv.org)
- Here, we show that young and aged LT-HSCs respond differently to inflammatory stress, such that aged LT-HSCs produce a cell-intrinsic, myeloid-biased expression program. (biorxiv.org)
- Using single-cell RNA-seq, we identify a myeloid-biased subset within the LT-HSC population (mLT-HSCs) that is much more common amongst aged LT-HSCs and is uniquely primed to respond to acute inflammatory challenge. (biorxiv.org)
- We predict several transcription factors to regulate differentially expressed genes between mLT-HSCs and other LT-HSC subsets. (biorxiv.org)
- Aged LT-HSCs demonstrate a cell-intrinsic myeloid bias during inflammatory challenge. (biorxiv.org)
- Altogether, the evidence of HSCs in ectopic bones growing at the expense of soft tissue in spinal cord/brain-injured patients indicates that inflammation and muscle contribute to HSC regulation by the brain-bone-blood triad. (jci.org)
- To corroborate these findings molecularly, we performed transcriptomic analyses and found significant upregulation of a distinct HSC gene expression signature in cells cultured with Delta1 in hypoxia (Fig. A). Collectively, these data show that hypoxia supports a superior ex vivo expansion of human HSCs with LTR activity compared with normoxia at optimized densities of Delta1. (confex.com)
- To clarify how hypoxia improved Notch-mediated expansion of LTR-HSCs, we performed scRNA-seq of CD34+ cells treated with Delta1 under normoxic or hypoxic conditions. (confex.com)
- Hypoxia lessened the cellular stress response in both progenitors and HSCs, but the mitigation was more apparent in the HSC population (Fig. C, grey plots), and decreased apoptosis was observed only within the HSC-enriched cluster 5 (Fig. D). These findings are consistent with several reports indicating that HSCs are more vulnerable to strong ER stress than downstream progenitors due to their lower protein folding capacity. (confex.com)
- However, human hematopoietic cells developed from HSCs are not always phenotypically and functionally identical to those in humans. (nih.gov)
- Several HOX transcription factors, including HOXB4, are highly expressed in hematopoietic stem cells (HSCs) but are downregulated upon differentiation towards more mature progeny cells. (dissertations.se)
- Culture conditions in which hematopoietic stem cells (HSCs) can be expanded for clinical benefit are highly sought after. (lu.se)
- To elucidate regulatory mechanisms governing the maintenance and propagation of human HSCs ex vivo, we screened libraries of annotated small molecules in human cord blood (CB) cells using an optimized assay for detection of functional HSCs during culture. (lu.se)
- Purified HSCs treated with CPX showed a reduced cell division rate and an enrichment of HSC- specific gene expression patterns. (lu.se)
- Our findings unveil a significant impact of iron homeostasis in regulation of human HSCs, with important implications for both basic HSC biology and clinical hematology. (lu.se)
Proliferation5
- The colony-forming unit (CFU) assay is a clonal, in vitro culture assay that measures the growth and frequency of functionally viable HSPCs by assessing the proliferation and differentiation of individual progenitor cells, resulting in the formation of discrete colonies in a semi-solid methylcellulose medium (such as MethoCult™ ) when supplemented with appropriate cytokines. (stemcell.com)
- We show that marrow from human NHOs contains hematopoietic stem cell (HSC) niches, in which mesenchymal stromal cells (MSCs) and endothelial cells provide an environment supporting HSC maintenance, proliferation, and differentiation. (jci.org)
- MTT assay was performed to determine the proliferation of cancer cells. (techscience.com)
- In addition, overexpression of ZYX in hepatoma cell lines (PLC/PRF/5, HCCLM3) enhanced their proliferation, migration and invasion, whereas ZYX knockdown had the opposite effects (SK HEP-1, Huh-7). (techscience.com)
- It is involved in the proliferation, invasion, apoptosis and epithelial mesenchymal transformation of malignant tumor cells. (techscience.com)
Vivo16
- This property forms the basis of in vivo assays of HSC function. (stemcell.com)
- In both in vitro and in vivo settings, steering pyruvate use toward glycolysis or OXPHOS differentially skews the hematopoietic output of HE cells toward either an erythroid fate with primitive phenotype, or a definitive lymphoid fate, respectively. (lu.se)
- In this study, we established an in vitro differentiation system yielding in vivo hematopoietic reconstitution hematopoietic cells from mouse PSCs through a 3D induction system followed by coculture with OP9 stromal cells. (biomedcentral.com)
- The c-kit + cells from 3D self-assembling peptide induction system followed by the OP9 coculture system possessed apparently superiority in terms of in vivo repopulating activity than that of 3D induction system followed by the 0.1% gelatin culture. (biomedcentral.com)
- Altogether, our findings lay a foundation for improving the efficiency of hematopoietic differentiation and generating in vivo functional HSC-like cells from mouse PSCs for clinical application. (biomedcentral.com)
- In fact, only using the OP9 co-culture system combined with hematopoietic related cytokines, we hardly obtained the in vivo functional reconstitution hematopoietic cells from PSCs [ 9 ]. (biomedcentral.com)
- In vivo models of human immunity in human tumor-bearing mice are frequently used to study mechanisms of targeted immunotherapies, safety, and efficacy. (genengnews.com)
- The Jackson Laboratory In Vivo Services offers oncology efficacy testing services using CD34+ HSC and PBMC humanized NSG TM and NSG TM variant strains, in addition to providing study-ready humanized mice to propel your research and discovery. (jax.org)
- Notch activation in human CD34+ hematopoietic stem/progenitor cells (HSPCs) by treatment with Delta1 ligand has enabled clinically relevant ex vivo expansion of short-term HSPCs. (confex.com)
- Mediates ex vivo expansion of cord blood CD34+ hematopoietic stem and progenitor cells (Huang et al. (stemcell.com)
- Activation of OCT4 enhances ex vivo expansion of human cord blood hematopoietic stem and progenitor cells by regulating HOXB4 expression. (stemcell.com)
- Although hematopoietic stem cells (HSC) are the best characterized and the most clinically used adult stem cells, efforts are still needed to understand how to best ex vivo expand these cells. (stemcell.com)
- These humanized mice facilitate the analysis of human hematology and immunology in vivo. (nih.gov)
- We found that the antifungal agent ciclopirox ethanolamine (CPX) selectively supported immature CD34+CD90+ cells during culture and enhanced their long-term in vivo repopulation capacity. (lu.se)
- This project will delve into innate immune cell function, establishing in vitro and in vivo systems test innate immune cell function in regeneration. (lu.se)
- Inspired from developmental processes, human mesenchymal cell lines can be programmed to form cartilage, bone and bone marrow tissues in vitro and in vivo. (lu.se)
Progenitor11
- However, there are two main principles by which cells facilitate therapeutic action: Stem, progenitor, or mature cell engraftment, differentiation, and long-term replacement of damaged tissue. (wikipedia.org)
- Sites of loosely packed extracellular matrix (ECM) also offer stem and progenitor cell optimal space to proliferate and differentiate. (europa.eu)
- Also making up a part of the hematopoietic system are short-term repopulating progenitor cells, which give rise to lineage-specific cell types. (stemcell.com)
- Collectively, these are referred to as hematopoietic stem and progenitor cells (HSPCs). (stemcell.com)
- Colonies derived from different types of progenitor cells are classified and counted based on morphological and phenotypic criteria. (stemcell.com)
- Although hematopoietic stem and progenitor cells (HSPCs) enable autologous treatment of blood disorders, de novo generation of hematopoietic cells from pluripotent stem cells still compromises the unlimited high yield and rich hematopoiesis engraftment potential [ 2 ]. (biomedcentral.com)
- We observed enhanced progenitor cell activity within the CD34+ cell population treated with Delta1 in hypoxia, but the benefits provided by low-oxygen cultures were most notable in the primitive HSC compartment. (confex.com)
- Most clusters could be computationally assigned to a defined hematopoietic subpopulation, including progenitor cells (clusters 0 to 4) and a single transcriptionally defined HSC population (cluster 5). (confex.com)
- This process relies on the formation of a blastema, a pool of progenitor cells that is formed in response to amputation. (lu.se)
- We know that innate immune cells are required for blastema progenitor cell formation, but are lacking an understanding of the immune cell processes and functions that are required for inducing and maintaining these progenitors cells. (lu.se)
- Likewise, these cells give rise to progenitor cells committed to a particular cell lineage, and play a crucial role in tissue repair and homeostasis. (bvsalud.org)
Allogeneic5
- Research Objective The expected outcome is a therapeutic candidate, allogeneic HSC-engineered HLA-I/II-negative human iNKT cells, that can potentially be used as an off-the-shelf cellular therapy for treating cancer. (ca.gov)
- Research Objective Development of hESC-derived pancreatic beta cells that are protected from allogeneic and autoimmune attack into a cell therapy for type 1 diabetes (T1D) Impact Cell therapy of T1D is challenged by immune rejection. (ca.gov)
- In 2021 Atara biotherapeutics became the first ever allogeneic T cell therapy company to be reviewed by any regulatory agency in the world (EMA) Cell therapy is targeted at many clinical indications in multiple organs and by several modes of cell delivery. (wikipedia.org)
- Allogeneic hematopoietic stem cell (HSC) is a curative treatment used for many hematologic malignancies and congenital or acquired anomalies of hematopoiesis. (pasteur.fr)
- Indoles derivatives are ligands of the receptor Aryl Hydrocarbon (AhR), which regulates the activation and differentiation of immune cells and could therefore contribute to limiting the risk of immune reaction to allogeneic origin of GVHD. (pasteur.fr)
Donor5
- Research Objective We will produce a universal donor cell (UDC) line by gene editing an embryonic stem cell line. (ca.gov)
- Currently we are collecting and growing epithelial cells from the ocular surface of donor eye tissues. (stanford.edu)
- Graft against the host (GVHD) is a major complication that may occur after a allograft, and is characterized by tissue damage induced by donor immune cells which react against those of the recipient. (pasteur.fr)
- these mice carry the SIRP-alpha gene similar to humans that allows the engraftment of donor cells. (genengnews.com)
- 2018). The response to pembrolizumab was variable by HSC donor, highlighting the importance of using multiple HSC donors across all arms in such studies (Wang et al. (jax.org)
Damaged tissue1
- These cells then integrate into the site of injury, replacing damaged tissue, and thus facilitate improved function of the organ or tissue. (wikipedia.org)
Biology9
- Nature Cell Biology 2022). (irbbarcelona.org)
- It thus contributes to the exploration of NK cells, and more generally of innate immune cells, in connection with specialised biology examinations as well as clinical research protocols. (univ-mrs.fr)
- Taking advantage of NSG TM and newly available variant strains engrafted with human immune cells will enable you to design highly targeted and human biology specific experiments. (jax.org)
- Caused by adenoviruses, they are non-enveloped DNA viruses that we use to study basic cell biology processes. (unm.edu)
- Adenoviruses are famous, in the sense that they taught us a lot about human biology - splicing, cancer studies - a lot of information came through the study of adenoviruses. (unm.edu)
- Rajaiya and her fellow scientists grow cells from the corneal tissue and then infect them with the virus and study the cell biology. (unm.edu)
- Cold Spring Harbor, NY -- Cold Spring Harbor Laboratory Press (CSHLP) announced the release of The Digital Cell: Cell Biology as a Data Science, available on its website in hardcover format. (cshlpress.com)
- Likewise, the knowledge of stem cell biology is crucial to the development of stem cell therapies, based on tissue engineering applied to dentistry, seeking the regeneration of dental tissues damaged or lost by caries, trauma or genetic diseases. (bvsalud.org)
- However, further studies are required to gain complete understanding of stem cell biology, which is fundamental for the development of successful cell-based therapies 1-3 . (bvsalud.org)
Haematopoietic stem cells1
- In the bone marrow MSC share the niche with haematopoietic stem cells (HSC). (europa.eu)
Assays4
- Colony-forming unit assays yielded denser cell colonies in the HA treated samples compared to the controls, yet no difference in the number of colonies was detected, indicating that the number of stem cells remained unchanged. (europa.eu)
- Cell counting and MTT assays were used to assess cell viability in the absence or presence of GSP. (spandidos-publications.com)
- Recently, enumeration and functionality assays of circulating and tissue-resident Innate Lymphoid cells were also developed. (univ-mrs.fr)
- In vitro collagen contraction assays tested biological activity on LX2 cells. (ibecbarcelona.eu)
Subsets6
- Our aim is to establish phenotypically and transcriptionally characterized different subsets of primary human ocular cells. (stanford.edu)
- Additional markers can be used to distinguish HSPC subsets within the CD34+ population and isolate HSPCs with different engraftment abilities and capacities to expand or generate mature blood cells in culture. (stemcell.com)
- The laboratory performs phenotypic and functional characterization of most leukocyte subsets with a focus on lymphoid cells. (univ-mrs.fr)
- Levels of total CD3 + T cells and CD4 + and CD8 + subsets in addition to other cell populations, such as CD19 + and CD16/56 + , were evaluated as well as cells from spleen, liver, bone marrow, lung, and lysed whole blood. (genengnews.com)
- Single-cell RNA-seq unmasked the existence of two subsets within the LT-HSC population that was apparent upon stimulation but not steady-state. (biorxiv.org)
- One of the LT-HSC subsets is more prevalent in young and the other in aged mice. (biorxiv.org)
Immune cells6
- pathomechanisms that regulate extravasation of damaging immune cells into surrounding tissues are poorly understood. (nature.com)
- Tissue infiltration by activated innate immune cells, including monocytes, macrophages, and neutrophils, if untreated, leads to rapidly progressive organ injury, that can involve many organ systems. (nature.com)
- CRL's CD34 + humanized NCG model, HuCD34-NCG, injected with umbilical cord blood-derived CD34 + cells shows robust engraftment of major human immune cells and demonstrates infiltration of T cells into tissues and xenograft tumors. (genengnews.com)
- For example, co-engrafting human tumors with human immune cells into the NSG TM (NOD.Cg- Prkdc scid Il2rg tm1Wjl /SzJ, JAX Stock# 005557 ) and its variant strains creates a powerful system to interrogate the immunomodulatory effects of human-specific experimental treatments in a preclinical setting. (jax.org)
- O nce we know how immune cells come into the eye in response to the virus, we can then use inhibitors to block these immune cells trafficking into the cornea, and possibly prevent blurred vision. (unm.edu)
- The three-dimensional culture is a very versatile system," Rajaiya says, "so once we know how immune cells come into the eye in response to the virus, we can then use inhibitors to block these immune cells trafficking into the cornea, and possibly prevent blurred vision. (unm.edu)
Mesenchymal2
- A particular stem cell type in the adult human-being is the 'Mesenchymal stem cell' (MSC). (europa.eu)
- Integrin alpha V and cortactin, but not epitheial-mesenchymal transition (EMT) transcriptional regulators, were up-regulated in the SWCNT-exposed cells, suggesting their role in the aggressive phenotype. (cdc.gov)
Pluripotent stem4
- Research Objective Functional human islet like organoids differentiated from human pluripotent stem cells. (ca.gov)
- Research Objective Universal Pluripotent Liver Failure Therapy (UPLiFT) is composed of 2 lines- UPLiFT0 ( from LiPSC-GR1.1) and UPLiFT1 which will be derived from gene edited universal human pluripotent stem cells. (ca.gov)
- Induced pluripotent stem cells, which harness the power of genetic reprogramming - basically, the altering of a cell's DNA - to change the course of cellular development. (articlecity.com)
- Oct-4 (POU5F1) is a transcription factor that is critically involved in the self-renewal of pluripotent stem cells, and its expression is commonly used as a marker for pluripotency. (stemcell.com)
Vitro8
- The recent development of stem cell research and the possibility of generating cells that can be stably and permanently modified in their genome open a broad horizon in the world of in vitro modeling. (wjgnet.com)
- In addition to the new in vitro systems, in recent years there were also significant advances in the development of new animal models that allows studying the entire cell cycle of human malaria. (wjgnet.com)
- Thanks to recent progresses, stem cells have been extensively employed to study Plasmodium liver and blood cycle in vitro . (wjgnet.com)
- Flow cytometry analysis, RNA-seq, and cell cycle analysis were used to detect the in vitro hematopoietic ability of endothelial protein C receptor (EPCR, CD201) cells generated in our induction system. (biomedcentral.com)
- Further, we innovatively demonstrated that notch signaling pathway is responsible for in vitro CD201 + hematopoietic cell induction from mouse PSCs. (biomedcentral.com)
- B ) Activated macrophages derived in vitro from NHO CD34 + cells stimulate NHO muscle-derived stromal cells (NHO-MDSCs). (jci.org)
- Adenoviral mediated overexpression of HOXB4 in human primitive hematopoietic cells in vitro generates very high levels of HOXB4 within 24 hours of transduction, leading to an increased differentiation towards myeloid lineages, and to a reduction of primitive hematopoietic progenitors, suggesting that the level of HOXB4 expression may determine the fate options following gene transfer. (dissertations.se)
- Understanding how stem cells behave in the niche is extremely important in order to extract these cells from their natural habitat, expand them in vitro and transplant the stem cells back to the patient, to repair and/or regenerate tissues and organs, with no risks to the individual's integrity. (bvsalud.org)
GVHD4
- The results of these studies highlight major changes in the human metabolome after allograft, associated with the patient's metabolism alterations and its microbiota, and could represent new potential therapeutic targets for the prevention or treatment of GVHD. (pasteur.fr)
- These results published in the journal Nature Communications highlight the major changes in the human metabolome after allograft, associated with alterations of the patient's metabolism or its microbiota, which may represent potential therapeutic targets new for the prevention or treating the graft against the host (GVHD). (pasteur.fr)
- JAX now offers a simplified approach to avoiding acute GvHD when using human PBMC's in these types of studies. (jax.org)
- A new NSG TM variant strain harboring null mutations in mouse MHC I and MHC II (NOD.Cg- Prkdc scid H2-Ab1 em1Mvw H2-K1 tm1Bpe H2-D1 tm1Bpe Il2rg tm1Wjl /SzJ, JAX Stock# 025216 ) has significantly delayed and diminished GvHD following human PBMC engraftment (Brehm et al. (jax.org)
Squamous Cell Carc1
- The risk is increased 1000-fold for squamous cell carcinoma, basal cell carcinoma, malignant melanoma, and fibrosarcoma and is increased 10-fold to 20-fold for other tumors. (medscape.com)
Adult5
- Stem cells exist both in embryos and adult cells. (articlecity.com)
- Adult stem cells, which are present in small amounts in adult tissue but less adaptable than embryonic stem cells, making their use in medical treatments more challenging. (articlecity.com)
- In the adult, HSC quiescence, maintenance, and differentiation are closely linked to changes in metabolism. (lu.se)
- Human adult mobilized CD34+ cells were cultured for 21 days under normoxia (21% O 2 ) or hypoxia (2% O 2 ) in vessels coated with optimized concentrations of Delta1. (confex.com)
- Some adult stem cell niches have already been described, but the majority of them remain unclear, including the dental pulp stem cell niches. (bvsalud.org)
Receptors3
- CD70 CAR-T cells were modified to express receptors for IL-8 to enhance intratumoral T cell migration as expression of this chemokine is increased in tumor tissue and can be stimulated by radiation. (jax.org)
- Human NK cells developed in hIL-2 NOG mice express various NK receptors and produce both granzyme A and perforin upon stimulation. (taconic.com)
- Specific delivery of ROCK-inhibitor Y-27632 (Y27) to HSC targeting mannose-6-phosphate-receptors reduces portal pressure and fibrogenesis. (ibecbarcelona.eu)
Reconstitution1
- These models display a robust and broad-spectrum human hematopoiesis, are suitable for large cohort generation, and are thoroughly standardized for reconstitution kinetics. (genengnews.com)
Lineages4
- We have observed engraftment of the cells and differentiation into multiple blood lineages, including T cells, B cells, and regulatory T cells. (ca.gov)
- We hypothesize that iPSC reprogrammed from ocular epithelial cells will be more efficient in differentiating into ocular lineages and exhibit higher functionality than non-ocular sources such as fibroblasts or blood. (stanford.edu)
- Here, we show that the emergence of blood is regulated by multiple metabolic pathways that induce or modulate the differentiation toward specific hematopoietic lineages during human EHT. (lu.se)
- At these sites, which are a compound of stromal cells, extracellular matrix and soluble factors, complex molecular interactions that maintain the essential properties of stem cells occur, such as self-renewal and differentiation into multiple lineages, according to the organism's needs. (bvsalud.org)
Gene expression1
- This study evaluates the effect of A. montana leaves hexane extract on several signaling cascades and gene expression in metastatic breast cancer cells upon insulin-like growth factor-1 (IGF-1) stimulation. (techscience.com)
Inflammation4
- However, in many diseases and disorders, cell are compromised by e.g. senescence, limited blood supply (ischemia), inflammation, or simply a reduction in the number of cells. (wikipedia.org)
- A role of Src family kinase members in causing sterile inflammation and human disease is only recently recognized. (nature.com)
- Klf5 , Ikzf1 and Stat3 regulate age‐ and inflammation-related LT-HSC myeloid-bias. (biorxiv.org)
- The pathways are important because how a virus enters the cells also dictates the downstream events, which then leads to cellular responses like the inflammation process. (unm.edu)
Immunodeficiency virus1
- Pneumocystis jirovecii Pneumonia Pneumocystis jirovecii is a common cause of pneumonia in immunosuppressed patients, especially in those infected with human immunodeficiency virus (HIV) and in those receiving systemic. (msdmanuals.com)
Hematopoiesis2
- We are also testing different routes of administration of these cells, including into the placenta, which is a site of hematopoiesis. (ca.gov)
- However, HOXB4 is not required for generation of HSC or maintenance of steady state hematopoiesis. (dissertations.se)
Regenerative Medicine2
- In an effort to advance the treatment strategies for the ocular surface diseases, I am focused on a regenerative medicine and single cell RNA sequencing based approaches to better comprehend the cellular biomarkers and molecular mechanisms involved in corneal tissue development and diseases. (stanford.edu)
- Applications in research involving cancer, infectious disease, immunology, regenerative medicine and human immune system engraftment. (taconic.com)
Chimeric antigen receptor3
- Human iPSC-derived chimeric antigen receptor expressing macrophages for improved cancer treatment. (ca.gov)
- Humanized NSG TM have also proven to be a valuable platform for assessment of anti-tumor T cell activity using both chimeric antigen receptor T cells (CAR-T) and bi-specific T cell engagers (BiTE). (jax.org)
- The study is an interdisciplinary effort to generate safe and effective chimeric antigen receptor (CAR)-T cell therapy for cancer that will be done by doing a deep-dive into understanding the relationship between the metabolism and anti-tumor functions of CAR-T cells. (uclahealth.org)
Engraftment of human cells2
- They robustly support the engraftment of human cells, and develop functional human immune, systems making them ideal for studying immune-related therapies in a vast array of applications. (genengnews.com)
- mouse: an excellent recipient mouse model for engraftment of human cells. (taconic.com)
Regulates2
- Mechanistically, they uncover a non-classical retinoic acid signaling axis that regulates HSC identity. (irbbarcelona.org)
- Abstract Fos-related antigen 1 (Fra-1) is a nuclear transcription factor that regulates cell growth, differentiation, and apoptosis. (techscience.com)
Mice14
- We are working with other HSC types, such as those derived from iPS cells, to determine whether they can engraft in these mice as well. (ca.gov)
- NOD-SCID-Gamma (NSG) mice) and measuring the presence of human blood cells in the blood or bone marrow (BM) after extended periods of engraftment, e.g., 20 weeks or longer. (stemcell.com)
- Humanized NCG mice have severely impaired murine T and B cells or innate immunity provided by macrophages and NK cells. (genengnews.com)
- They were able to suppress tumor growth in lung, sarcoma, bladder, breast cancer PDX, and a breast cancer cell line when co-engrafted in CD34+ hematopoietic stem cell (HSC) humanized NSG TM mice (Wang et al. (jax.org)
- 2018). In another experiment, treatment with an antibody directed against the costimulatory molecule GITR in CD34+ HSC-engrafted NSG TM mice co-engrafted with the human melanoma cell line SK-MEL-5 suppressed tumor growth and reduced intratumoral T regulatory cell number concurrent with increased activation of CD8+ T cells, mirroring what had been observed in syngeneic model systems (Mahne et al. (jax.org)
- In another study, efficacy of a BiTE was assessed in NSG TM mice engrafted with hematological cancers known to express the intracellular tumor marker WT1, where an epitope of the tumor marker is expressed on the cell surface in the context of the MHC I molecule HLA-A2 (Dao et al. (jax.org)
- Physiologic aging in both humans and mice leads to permanent changes in LT-HSC function, such as myeloid-biased hematopoietic output ( Akunuru and Geiger, 2016 ). (biorxiv.org)
- hIL-2 NOG mice produce human IL-2 in the range of 0.5-2.0 ng/mL as measured by MFI serology testing on DBS and serum in February 2023. (taconic.com)
- The hIL-2 NOG mouse was developed by microinjecting a transgene consisting of a DNA fragment containing human IL-2 cDNA under the control of the CMV promoter into zygotes of NOD/ShiJic-Il2rg mice. (taconic.com)
- Fukuchi Y, Miyakawa Y, Kobayashi K, Kuramochi T, Shimamura K, Tamaoki N, Nomura T, Ueyama Y, Ito M. (1998) Cytokine dependent growth of human TF-1 leukemic cell line in human GM-CSF and IL-3 producing transgenic SCID mice. (taconic.com)
- Highly immunodeficient mice, which do not reject xenografts and support cell and tissue differentiation and growth, are indispensable for generating additional appropriate models. (nih.gov)
- These mice were generated by genetically introducing human cytokine genes into NOD/SCID/γc(null) and Rag2(null)γc(null) mice. (nih.gov)
- In this review, we describe the current knowledge of human hematopoietic cells developed in these mice. (nih.gov)
- Various human disease mouse models using these humanized mice are summarized. (nih.gov)
Clinical4
- Impact Providing the immediate cell therapeutic candidate for clinical trial of diabetic patients. (ca.gov)
- Core resources include state-of-the-art facilities and services for rodent behavioral testing, animal PET/MRI, optical and electrophysiology, cell and molecular, image analysis, neuropsychological testing and human subjects' clinical assessment. (unm.edu)
- Scientists working in basic, translational, and clinical cancer metabolism research are invited to join the Academy in New York on April 17th to discuss the intersection between cell signaling and metabolism. (nyas.org)
- Development of pre-clinical models using stem cell technologies. (nottingham.ac.uk)
Metabolism2
- Alejo Rodriguez-Fraticelli, Ph.D - Group Leader , Quantitative Stem Cell Dynamics, Aging and Metabolism Programme. (irbbarcelona.org)
- Mechanistically, we found that the HSC stimulating effect of CPX was directly mediated by chelation of the intracellular iron pool, which in turn affected iron-dependent proteins and enzymes mediating cellular metabolism and respiration. (lu.se)
Complications4
- Interestingly, the authors developed and used an Epstein Barr Virus-specific human T cell population to prevent complications with Graft Versus Host Disease (Dao et al. (jax.org)
- 2019). This platform offers a significant advantage in immuno-oncology studies requiring mature human T cells by enabling much longer study duration and fewer study complications. (jax.org)
- Phagocytic cell defects Immunodeficiency disorders are associated with or predispose patients to various complications, including infections, autoimmune disorders, and lymphomas and other cancers. (msdmanuals.com)
- HSCT make it an aggressive process, causing consists of painless intravenous infusion of healthy toxicity and can generate significant complications hematopoietic stem cells (HSC) extracted from bone and several side effects adding feelings of worry, marrow (BM), peripheral blood (PB) and placental anguish, anxiety, among others (KUBA et al. (bvsalud.org)
Murine1
- One sentence summary Murine hematopoietic stem cells display transcriptional heterogeneity that is quantitatively altered with age and leads to the age-dependent myeloid bias evident after inflammatory challenge. (biorxiv.org)
Regeneration3
- Stem cells are essential for regeneration and repair of worn-out or injured tissue. (europa.eu)
- With this raw material, biomedical firms can create stem cell lines that, among other things, aid recuperation via the regeneration of tissue that has been lost or damaged. (articlecity.com)
- Therapies based on the application of stem cells have great potential in the prevention and treatment of several diseases, such as cancer, diabetes, cardiovascular disease, spinal cord injuries, neurological diseases such as Parkinson's and Alzheimer's, and in the regeneration of various tissues and organs. (bvsalud.org)
Differentiate4
- In this paradigm multipotent or unipotent cells differentiate into a specific cell type in the lab or after reaching the site of injury (via local or systemic administration). (wikipedia.org)
- They are able to differentiate into any cell of an organism and have the ability of self-renewal. (articlecity.com)
- A ) CD34 + cells from human neurogenic heterotopic ossification (NHO) were isolated using immunomagnetic cell separation and induced to differentiate into macrophages for 2 weeks in specific medium. (jci.org)
- Dental pulp stem cells have been isolated from deciduous and permanent teeth and have the potential to self-renew and differentiate. (bvsalud.org)
Niches1
- Niches are special microenvironments in tissue where stem cells are located. (bvsalud.org)
Differentiation of human2
- Activated macrophages contribute to the osteogenic differentiation of human NHO-MDSCs. (jci.org)
- Predominant differentiation of human NK cells following human HSC engraftment, with>10-fold higher CD56+ NK cell numbers compared to the base NOG mouse. (taconic.com)
Genes2
- Found within Wharton's jelly - which is easily harvested from what would otherwise be post-natal medical waste - are several distinct stem cell genes. (articlecity.com)
- Pathobiology: Cells and Genes;Cellular and Molecular Haematology. (nottingham.ac.uk)
Stromal cells1
- We demonstrate that oncostatin M (OSM) produced by activated macrophages promotes osteoblastic differentiation and mineralization of human muscle-derived stromal cells surrounding NHOs. (jci.org)
Demonstrate1
- Human specimen control (HSC): A human cell culture preparation used as an extraction procedural control to demonstrate successful recovery of nucleic acid, as well as extraction reagent integrity. (cdc.gov)
Embryonic3
- Embryonic stem cells, which must be extracted from embryos three to five days old (known as blastocysts, which contain only about 150 cells at this point of development). (articlecity.com)
- They have many of the positive characteristics of embryonic stem cells while sourcing material is far less challenging, since postpartum tissue can be used. (articlecity.com)
- We explore the method on three circuits for haematopoiesis and embryonic stem cell development for commitment and reprogramming scenarios and illustrate how the method can be used to determine sequential steps for onsets of external factors, essential for efficient reprogramming. (lu.se)
Flow Cytometry6
- Detection of Integrin alpha 2b/CD41 in Human Blood Platelets by Flow Cytometry. (rndsystems.com)
- A QC screening flow cytometry panel and a more extensive T cell and myeloid panel identify the presence of human cell populations and evaluate the model's functionality. (genengnews.com)
- Flow cytometry analysis of peripheral blood at 12 and 16 weeks post injection verified engraftment of human cell subpopulations. (genengnews.com)
- Cells were then analyzed by flow cytometry for expression of myeloid marker CD11b, monocyte/macrophage marker CD14, and M2 macrophage activation marker CD163 and CD206. (jci.org)
- C ) Mononuclear cells from human NHO marrows were analyzed by flow cytometry for mac markers, as in A . ( D ) Medium conditioned by activated macrophages from human NHO stimulate NHO-MDSC mineralization. (jci.org)
- Propidium iodide staining and flow cytometry analysis of Annexin V binding was utilized to measure the progression of the cell cycle and the induction of apoptosis. (techscience.com)
Enrichment1
- To assess the relative impact of normoxia and hypoxia on the HSC compartment, we performed gene set enrichment analysis (GSEA) of cells within HSC cluster 5 from each culture condition. (confex.com)
Tissues3
- This special type of stem cell is present in many tissues and restores bone, connective tissues and fat. (europa.eu)
- The knowledge gained from separating and identifying different cells types of the eye will provide greater understanding into the physiology and overall function of the various eye tissues. (stanford.edu)
- Here, we reported that mammalian target of rapamycin complex 2 (mTORC2) kinase is overexpressed in pancreatic cancer tissues and promotes pancreatic cancer cell growth and invasion. (techscience.com)
Generate2
- Research Objective We propose to generate NK cells with enhanced immunity from gene-edited human PSCs and use the resultant NK cells to kill SARS-CoV-2-infected cells to combat against COVID-19. (ca.gov)
- that is, they have the ability to generate other stem cells and perpetuate themselves. (bvsalud.org)
Endothelial cells2
- Functional studies revealed increased expression of ICAM-1 on induced patient-derived endothelial cells (iECs) and of β2-integrins on patient neutrophils that increase neutrophil adhesion and vascular transendothelial migration (TEM). (nature.com)
- We characterize the role of increased Lyn kinase activity in neutrophils, endothelial cells and lesional liver biopsies and utilized an iPSC-derived endothelial cell platform for disease modeling of neutrophilic vasculitis and to screen and evaluate drug efficacy. (nature.com)
Oxidative stress1
- The perturbances in cellular redox homeostasis leads to high free radical burden thereby causing oxidative stress in the cells. (stanford.edu)
Therapeutic4
- In more recent work, cell encapsulation is pursued as a means to shield therapeutic cells from the host immune response. (wikipedia.org)
- Cell therapy provided a novel approach to effectuate therapeutic efficacy. (wikipedia.org)
- Dendritic cells-based therapeutic approach may be a potential strategy for SARS-CoV-2 infection. (medsci.org)
- Our results provide strong evidence that macrophages contribute to NHO formation through the osteogenic action of OSM on muscle cells within an inflammatory context and suggest that OSM/OSMR could be a suitable therapeutic target. (jci.org)
HSPCs1
- CD34 is a transmembrane phosphoglycoprotein that was first identified on HSPCs, but its expression is also present on other cell types, such as vascular tissue. (stemcell.com)
Findings1
- The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. (cdc.gov)
Liver5
- Fibrotic livers are characterized by an inflammatory microenvironment that is composed of various immunologically active cells, including liver-resident populations (e.g. (mdpi.com)
- After liver injury, recruited inflammatory cells accumulate in the damaged site. (spandidos-publications.com)
- The technology involves capturing vesicles released from liver cancer cells from patient plasma, and measuring molecules inside the vesicles that can inform us about the stage of the cancer. (uclahealth.org)
- Since in cirrhosis, platelet-derived growth factor receptor beta (PDGFRβ) is upregulated in the liver as well as the kidney, this study coupled Y27 to human serum albumin (HSA) substituted with PDGFRβ-recognizing peptides (pPB), and investigated its effect on PTH in cirrhotic rats. (ibecbarcelona.eu)
- Y27pPBHSA was found in the liver of rats up to 6 hours after its injection, in the HSC demonstrated by double-immunostainings. (ibecbarcelona.eu)
Functional2
- Katano I, Takahashi T, Ito R, Kamisako T, Mizusawa T, Ka Y, Ogura T, Suemizu H, Kawakami Y, Ito M. (2015) Predominant development of mature and functional human NK cells in a novel human IL-2-producing transgenic NOG mouse. (taconic.com)
- In X-linked hyper-IgM syndrome, T cells lack functional CD40 ligand and cannot signal B cells to switch. (msdmanuals.com)
Acute4
- In general, for patients presenting damaged or destroyed bone marrow, for example after chemotherapy and/or radiation for acute myeloid leukemia (AML), bone marrow derived cells can be infused into the patients blood stream. (wikipedia.org)
- Inhibition of HOX/PBX dimer formation leads to necroptosis in acute myeloid leukemia cells ONCOTARGET. (nottingham.ac.uk)
- In the past dozen years, the outbreak of the novel coronavirus is the third on record spillover of an animal coronavirus to humans after occurring of severe acute respiratory syndrome (SARS) started in 2002 and Middle East respiratory syndrome coronavirus (MERS-CoV) in 2014, which is resulting in a pandemic in global. (medsci.org)
- Young children have acute lymphoblastic leukemia of T-cell origin. (medscape.com)
Bone marrow derived cells1
- Following wounding, endogenously secreted TGFßs drive resident and bone marrow-derived cells to convert into α-smooth actin (SMA)-rich, contractile myofibroblasts. (bvsalud.org)
Transcriptomic Signature1
- The transcriptomic signature of MSCs from NHOs shows a neuronal imprinting associated with a molecular network required for HSC support. (jci.org)
Distinct2
- Today, it has become a distinct source of stem cells and, therefore, a critical element in advancing medical treatments for everything from hair loss, the treatment of wounds, and surgical procedures. (articlecity.com)
- We identified 6 distinct clusters (clusters 0 to 5) in dimension-reduction (UMAP) analysis, with a comparable distribution of cells per cluster between normoxic and hypoxic cultures. (confex.com)
Epithelial4
- We intend to harness the inherent epigenetic memory of the ocular epithelial cells for superior and quicker protocol to regenerate cornea. (stanford.edu)
- Cornea is constantly regenerated from a small population of limbal epithelial stem cells via transiently amplifying cell (TAC) states. (stanford.edu)
- Employing this specific strategy, we wish to explore the transdifferentiated or partially reprogrammed state in ocular epithelial cell types by giving pulsed reprogramming factors. (stanford.edu)
- In pilot studies, CRL evaluated the antitumor effects of immune checkpoint inhibitors (CPIs) on colon epithelial carcinoma (RKO) and basal lung cell adenocarcinoma (A549) cell lines in the HuCD34-NCG model and showed significant immunomodulatory antitumor response. (genengnews.com)