AcetylationInhibitorsP300ProteinsInhibitor of histone acetyltransferasesSubunitInhibitionProteinChromatin structureCore histonesGCN5HATsNucleosomeMRNALysinesPeptideAcetyl groupModificationEnzymeNucleosomalYeastCofactorMethylationNucleosomesTranscriptional regulationMultisubunitEpigeneticsActivityBladder cancerMutationsAssayFunctionalInactivationGenesRegulatesChromosomeRegulation of gene exPCAFDetectsResearchersComplex
Acetylation14
- Post-translational acetylation of the histone H4 N-terminal tail in chromatin has been associated with several nuclear processes including transcription. (wikipedia.org)
- Acetylation and deacetylation of histones, carried out by histone acetyltransferases (HATs) and histone deacetylases (HDACs), respectively, affects cellular division, differentiation, death and survival. (lu.se)
- We found acetylation of histones and probably other proteins to be dramatically reduced in degenerating photoreceptors in the rd1 human homologous mouse model for RP. (lu.se)
- Acetylation of histone H4 by p300 was more sensitive to inhibition by garcinol compared with that of H3. (enzolifesciences.com)
- Histone H4 acetylation was completely inhibited at 1 µM while acetylation of H3 was not abolished at 20 µM. (enzolifesciences.com)
- and they are associated with altered histone acetylation and transcription factor (nerve growth factor-induced clone A [NGFIA]) binding to the glucocorticoid receptor promoter. (deepdyve.com)
- Molecular Basis for Cohesin Acetylation by Establishment of Sister Chromatid Cohesion N-Acetyltransferase ESCO1. (genscript.com)
- these studies reveal the molecular basis for SMC3 acetylation by ESCO1 and have broader implications for understanding the structure/function of non-histone acetyltransferases. (genscript.com)
- Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. (cancerindex.org)
- In htz1Delta cells, Sir2 and Sir3 spread into flanking euchromatic regions, producing changes in histone H4 acetylation and H3 4-methylation indicative of ectopic heterochromatin formation. (sdbonline.org)
- In the beginning, CPTH2 selectively inhibits the acetylation of histone H3 by recombinant human Gcn5 in a substrate-competitive manner. (cancer-research-network.com)
- In vivo, it decreased acetylation of bulk histone H3 at the specific H3-AcK14 site. (cancer-research-network.com)
- Although originally termed histone acetyltransferases (HATs) for their lysine acetylation activity on histone N-terminal tails, CBP and its paralogue, p300, have been shown to acetylate a variety of non-histone proteins including p53, DNA polymerase β and nuclear import factors. (enzolifesciences.com)
- Acetylation is an important, reversible post-translational modification affecting histone and non-histone proteins with critical roles in gene transcription, DNA replication, DNA repair, and cell cycle progression. (oregonstate.edu)
Inhibitors5
- The application, filed in April 2016, is focused on using histone deacetylase (HDAC) inhibitors and/or histone acetyltransferases (HAT) activators to treat heart failure in humans. (unc.edu)
- Assay interference and off-target liabilities of reported histone acetyltransferase inhibitors. (reactionbiology.com)
- Together with the powerful apoptotic effect (particularly notable if considering that anacardic acid and other KAT inhibitors are not cell permeable) appoint them as valuable biological tools to understand the mechanisms of lysine acetyltransferases. (cnr.it)
- Histone deacetylase and acetyltransferase inhibitors decrease organic dust-mediated activation of proinflammatory gene expression. (cdc.gov)
- Histone and Non-Histone Targets of Dietary Deacetylase Inhibitors. (oregonstate.edu)
P3007
- It is a potent inhibitor of histone acetyltransferases (HATs) p300 (IC 50 =7µM) and PCAF (IC 50 =5µM) both in vitro and in vivo . (enzolifesciences.com)
- Citation: Ding Z, Cao J, Shen Y, Zou Y, Yang X, Zhou W, Guo Q and Huang C (2018) Resveratrol Promotes Nerve Regeneration via Activation of p300 Acetyltransferase-Mediated VEGF Signaling in a Rat Model of Sciatic Nerve Crush Injury. (vinomis.com)
- In the affected ventral spinal cord, resveratrol enhanced the expression of several vascular endothelial growth factor family proteins (VEGFs) and increased the phosphorylation of p300 through Akt signaling, indicating activation of p300 acetyltransferase. (vinomis.com)
- Inactivation of p300 acetyltransferase reversed the resveratrol-induced expression of VEGFs and motor repair in rats that had undergone sciatic nerve crush injury. (vinomis.com)
- Resveratrol activated p300 acetyltransferase-mediated VEGF signaling in the affected ventral spinal cord, which may have thus contributed to the acceleration of nerve regeneration and motor repair. (vinomis.com)
- The K-histone acetyltransferase KAT3B (p300) could play a role. (cancer-research-network.com)
- In addition, CPTH2 decreases the invasiveness of a clear cell renal carcinoma (ccRCC) cell line through the inhibition of acetyltransferase p300 (KAT3B). (cancer-research-network.com)
Proteins9
- These enzymes modify histones, which are structural proteins that attach (bind) to DNA and give chromosomes their shape. (medlineplus.gov)
- To determine the reason for this species specificity, chimeras were constructed linking three structurally independent regions of the TFIIS proteins from yeast and human cells. (embl.de)
- The histone H2A.F/Z (H2AZ) variant is a functionally distinct, highly conserved histone subgroup that likely represents a separate evolutionary lineage of histone H2A proteins. (sdbonline.org)
- Both genes are highly conserved, and their proteins are thought to have 2 functions: (1) formation of a bridge or scaffold between the DNA-binding transcription factors and the RNA polymerase II complex and (2) serving as histone acetyltransferases that open the chromatin structure, a process essential for gene expression. (medscape.com)
- With ~1750 lysine-acetylated proteins in the human proteome, Reaction Biology's histone acetyltransferase assays provide an important advantage in terms of assay flexibility as well as gold standard quality. (reactionbiology.com)
- Histone acetyltransferases acetylate lysines on histones and other proteins using tritium-labeled acetyl-Coenzyme A as the acetyl donor. (reactionbiology.com)
- RATIONALE: Post-translational modification of histone proteins are major mechanisms that modify chromatin structure and regulate gene expression in eukaryotes. (cdc.gov)
- Out of this body of research, attention increasingly has shifted towards non-histone targets of HDACs and HATs, such as transcriptions factors, hormone receptors, DNA repair proteins, and cytoskeletal components. (oregonstate.edu)
- By virtue of their effects on both histone and non-histone proteins, dietary chemopreventive agents modulate the cellular acetylome in ways that are only now becoming apparent. (oregonstate.edu)
Inhibitor of histone acetyltransferases1
- Variations of the alkyl chain length can change the activity profile from inhibition of both KAT3A/KAT2B (as derivative 2a) to the peculiar profile of pentadecylidenemalonate 1b, the first activator/inhibitor of histone acetyltransferases. (cnr.it)
Subunit5
- In yeast, the complex has 13 subunits, including the catalytic subunit Esa1 (homologous to human Tip60). (wikipedia.org)
- Purification and characterization of a native multi-subunit complex (NuA4) from yeast that acetylates nucleosomal histone H4 have been reported. (wikipedia.org)
- Additionally, another subunit of the complex has been identified as the product of TRA1, an ATM-related essential gene homologous to human TRRAP, an essential cofactor for c-Myc- and E2F-mediated oncogenic transformation. (wikipedia.org)
- Orthologous to human NAA60 (N-alpha-acetyltransferase 60, NatF catalytic subunit). (nih.gov)
- Schizosaccharomyces pombe YEATS family histone acetyltransferase subunit Yaf9 (yaf9), mRNA. (genscript.com)
Inhibition1
- Activation of histone acetyltransferase (HAT) or inhibition of histone deacetylases (HDAC) is generally believed to allow chromatin to assume a more open state, permitting transcriptional activity. (cdc.gov)
Protein12
- The NuA4 histone acetyltransferase complex is a protein complex that has histone acetylase activity on chromatin, as well as ATPase, DNA helicase and structural DNA binding activities. (wikipedia.org)
- Protein interacting with PcG histone methylation sites may provide sites for therapeutic intervention. (chicagobiomedicalconsortium.org)
- Orthologous to human CREBBP (CREB binding protein). (zfin.org)
- The Histone acetyltransferases (HATs) of the MYST family are highly conserved from yeast to humans and function exclusively in multisubunit protein complexes [ 6 ]. (biomedcentral.com)
- Immunohistochemical images of cancer and normal tissue were downloaded from Human Protein Atlas ( http://www.proteinatlas.org/ ). (biomedcentral.com)
- The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family. (cancerindex.org)
- It has histone acetyl transferase activity with core histones and nucleosome core particles, indicating that this protein plays a direct role in transcriptional regulation. (novusbio.com)
- This gold standard approach can be used regardless of whether the substrate is a peptide, histone, nucleosome, or non-histone protein. (reactionbiology.com)
- RESULTS: We report here the surprising observation that treatment of a human bronchial epithelial cell line, BEAS-2B cells, with the inhibitor histone deacetylase, trichostatin A, or the histone acetyltransferase inhibitor, anacardic acid, strongly inhibited induction of both IL-6 and IL-8 protein upon stimulation with organic dust. (cdc.gov)
- E7 from human papil omavirus type 16 cooperate to cancer: the European prospective investigation into doi:10.1093/carcin/bgp321 PMID:20047954 target the PDZ protein Na/H exchange regulatory cancer and nutrition study. (who.int)
- Tat protein is a major factor of HIV (human immunodeficiency virus) transcription regulation and has other activities. (crie.ru)
- Shukla V, Cuenin C, Dubey N, Herceg Z. (2011) Loss of histone acetyltransferase cofactor transformation/transcription domain-associated protein impairs liver regeneration after toxic injury. (who.int)
Chromatin structure1
- These results describe a novel pathway for regulating transcription using variant histones to modulate chromatin structure (Santisteban, 2000). (sdbonline.org)
Core histones2
- Mutations in the major core histones relieve transcriptional repression and bypass the requirement for SNF/SWI and SAGA. (sdbonline.org)
- To counteract constraints imposed by nucleosome structure, cells deploy two major classes of multiprotein enzymes, which covalently modify the nucleosome core histones or catalyze nucleosome mobility in an adenosine triphosphate (ATP)-dependent fashion. (sdbonline.org)
GCN52
- Be the first to review Human KAT2A/GCN5 (NP_066564.2) VersaClone cDNA and earn rewards! (rndsystems.com)
- Have you used Human KAT2A/GCN5 (NP_066564.2) VersaClone cDNA? (rndsystems.com)
HATs4
- Histone acetyltransferases (HATs) of the MYST family are associated with a variety of human cancers. (biomedcentral.com)
- Histone acetyltransferase (HATS) is an enzyme that acetylates the conserved lysine amino acids in histones by transferring acetyl from acetyl coenzyme A toε-N-acetyllysine. (cancer-research-network.com)
- Specifically, HATS add lysine at the N-terminal of acetyl histones H3 and H4 to specific groups. (cancer-research-network.com)
- Key regulatory enzymes include histone deacetylase (HDACs) and histone acetyltransferases (HATs). (oregonstate.edu)
Nucleosome1
- This study has found that Swr1, a Swi2/Snf2-related adenosine triphosphatase, is the catalytic core of a multisubunit, histone-variant exchanger that efficiently replaces conventional histone H2A with histone H2AZ in nucleosome arrays. (sdbonline.org)
MRNA2
- EDN3 mRNA expression was assessed by Northern blotting in normal human tissues (n = 9) as well as in matched pairs of normal and tumourous tissues from breast specimens (n = 50). (biomedcentral.com)
- E) Embryos were injected at the two-cell stage with mE2a-GFP- and RFP-tagged histone H2b mRNA and monitored for fluorescence at stage 10.5. (xenbase.org)
Lysines1
- All four conserved lysines of histone H4 can be acetylated by NuA4. (wikipedia.org)
Peptide1
- 250 pmol/min/µg assayed as production of CoA-SH from AcCoA in the presence of a peptide comprising human p53 residues 368-386 (Prod. (enzolifesciences.com)
Acetyl group2
- By adding a small molecule called an acetyl group to particular locations on histones, histone acetyltransferases control the activity of certain genes. (medlineplus.gov)
- The tritium-acetyl group is transferred onto the histone substrate that is measured directly to reflect the enzyme activity. (reactionbiology.com)
Modification3
- These changes likely alter histone modification, which could prevent normal regulation of gene activity. (medlineplus.gov)
- Nucleosomes impose a block to transcription that can be overcome in vivo by remodeling complexes such as SNF/SWI and histone modification complexes such as SAGA. (sdbonline.org)
- Chromatin immunoprecipitation assay was performed to evaluate the histone modification of gene loci that are regulated by lunasin and cytokine. (weeksmd.com)
Enzyme2
- The KAT6B gene provides instructions for making a type of enzyme called a histone acetyltransferase. (medlineplus.gov)
- These mutations lead to the production of a shortened histone acetyltransferase enzyme. (medlineplus.gov)
Nucleosomal1
- Much of the current understanding of these processes is derived from analyses of nucleosomal histones that represent the major histone species within cells. (sdbonline.org)
Yeast3
- Domain I is conserved in evolution from yeast to human species and is homologous to the transcription factors elongin A and CRSP70. (embl.de)
- Histone variant H2A.Z (Htz1 in yeast) replaces conventional H2A in many nucleosomes. (sdbonline.org)
- Swr1 is required for the deposition of histone H2AZ at specific chromosome locations in vivo, and Swr1 and H2AZ commonly regulate a subset of yeast genes. (sdbonline.org)
Cofactor1
- Histone acetyltransferase assays performed at Reaction Biology are radiometric activity assays using tritiated acetyl-Coenzyme A as a cofactor. (reactionbiology.com)
Methylation2
- PCR2 targets genes that are developmentally regulated and catalyzes di- and histone tri-methylation, resulting in chromatin compaction and gene repression. (chicagobiomedicalconsortium.org)
- Epigenetic information can be transmitted by several different molecular mechanisms, which include but are not limited to DNA methylation and histone post-translational modifications (PTMs). (plos.org)
Nucleosomes2
- How histone variants such as H2AZ are incorporated into nucleosomes has been obscure. (sdbonline.org)
- These advances establish a third mechanism of chromatin reconfiguration, raising fundamental questions about the stability of nucleosomes in nonreplicative phases of the cell cycle and the cellular machinery responsible for incorporating histone variants into nucleosomes (Mizuguchi, 2004). (sdbonline.org)
Transcriptional regulation2
- Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. (cancerindex.org)
- Histone lysine acylation is a major class of histone post-translational modifications involved in essential biological activities, such as transcriptional regulation, DNA-damage, a repairnd cell-cycle progression. (news-medical.net)
Multisubunit1
- Human transcription factor IIIC (hTFIIIC) is a multisubunit complex that mediates transcription of class III genes through direct recognition of promoters (for tRNA and virus-associated RNA genes) or promoter-TFIIIA complexes (for the 5S RNA gene) and subsequent recruitment of TFIIIB and RNA polymerase III. (embl.de)
Epigenetics2
- The program focused on the role of epigenetics in biology and human diseases. (chicagobiomedicalconsortium.org)
- Several voices have questioned this use of the word epigenetics, because many histone PTMs do not carry heritable information [1] - [8] . (plos.org)
Activity2
- Antibodies against Esa1p specifically immunoprecipitate NuA4 activity whereas the complex purified from a temperature-sensitive esa1 mutant loses its acetyltransferase activity at the restrictive temperature. (wikipedia.org)
- It has histone deacetylase activity and represses transcription when tethered to a promoter. (cancerindex.org)
Bladder cancer1
- Increased expression of ESCO1 is correlated with poor patient survival and its role in human bladder cancer. (genscript.com)
Mutations2
- Studies suggest that these mutations likely prevent the production of functional histone acetyltransferase from one copy of the KAT6B gene in each cell. (medlineplus.gov)
- Disruption of the human CBP gene, either by gross chromosomal rearrangements or by point mutations, leads to Rubinstein-Taybi syndrome. (medscape.com)
Assay1
- All our histone acetyltransferase assay services are performed at our screening facility in Pennsylvania, US. (reactionbiology.com)
Functional1
- Cloning and characterization of two evolutionarily conserved subunits (TFIIIC102 and TFIIIC63) of human TFIIIC and their involvement in functional interactions with TFIIIB and RNA polymerase III. (embl.de)
Inactivation2
- EDN3 is a frequent target of epigenetic inactivation in human breast cancer, potentially contributing to imbalanced EDN signalling commonly found in this disease. (biomedcentral.com)
- 2012) Inactivation of the putative suppressor gene DOK1 by promoter hypermethylation in primary human cancers. (who.int)
Genes1
- Data show that acetyltransferase Esco1 colocalizes with cohesin throughout the cell cycle and down-regulates expression of neighboring genes. (genscript.com)
Regulates1
- The variant histone H2A.Z regulates gene transcription, and deletion of the gene encoding H2A.Z strongly increases the requirement for SNF/SWI and SAGA. (sdbonline.org)
Chromosome1
- Recent studies have revealed that variants of histone H2A and histone H3 play important roles not only in gene expression but also in the repair of DNA breaks and the assembly of chromosome centromeres. (sdbonline.org)
Regulation of gene ex1
- The conserved histone variant H2AZ has an important role in the regulation of gene expression and the establishment of a buffer to the spread of silent heterochromatin. (sdbonline.org)
PCAF1
- Western Blot: PCAF Antibody (5E11) [H00008850-M04] - Analysis of PCAF expression in human uterus myoma. (novusbio.com)
Detects1
- Salistick detects pregnancy by identifying a hormone called human chorionic gonadotropin (hCG), which is present in the body of a pregnant person. (medicaldaily.com)
Researchers1
- Researchers develop CRISPR-human organoids-single-cell RNA sequencing system to identify developmental brain defects in autism. (news-medical.net)
Complex1
- Predicted to be part of histone acetyltransferase complex and transcription regulator complex. (zfin.org)