• Employing IL-3 + SCF stimulated human cord blood CD34 + 38 - cells, we isolated and characterized different components of this current. (bgu.ac.il)
  • Inhibition of the expression of each of the channels suppressed progenitor cell generation by IL-3 and SCF-stimulated CD34 + 38 - cells in 7- day suspension cultures. (bgu.ac.il)
  • We first optimized a serum-free, miniature erythroid differentiation system starting from primary human CD34 cells, the exact type of cells we would ultimately like to target in vivo ( Figure 1 ). (haematologica.org)
  • Human CD34 + hematopoietic stem and progenitor cells purified from umbilical cord or adult peripheral blood were cultured in a two-phase liquid culture system in a serum-free medium for 21 days. (haematologica.org)
  • Interferon-gamma-mediated pathways are induced in human CD34(+) haematopoietic stem cells. (bsb-bg.eu)
  • In the present study, we demonstrate that functional CD34 + progenitor cells can be efficiently produced from human embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs) by combined modulation of 2 signaling pathways. (ashpublications.org)
  • hPSC-derived CD34 + progenitor cells further developed to endothelial and smooth muscle cells with functionality. (ashpublications.org)
  • Our results suggest that combined modulation of signaling pathways may be an efficient means of differentiating hPSCs into functional CD34 + progenitor cells. (ashpublications.org)
  • The CXCL12-3'A allele is associated with a higher mobilization yield of CD34 progenitors to the peripheral blood of healthy donors for allogeneic transplantation. (cdc.gov)
  • Hematopoietic stem cells (HSC) give rise to all types of blood lineages, including red blood cells (RBC). (lu.se)
  • Hematopoietic stem/progenitor cells (HSPC) are known to be functionally diverse in terms of their self-renewal potential and lineage output. (lu.se)
  • erythroid cells after in vitro differentiation, despite having similar frequencies of phenotypic HSC initially. (lu.se)
  • A potassium inward rectifier (K(it)) current was previously shown by us to be induced in primitive hematopoietic progenitor cells, stimulated with the combination of interleukin-3 (IL-3) and stem cell factor (SCF). (bgu.ac.il)
  • The variable expression of two essential inward rectifying potassium channels early in the course of hematopoietic progenitor cell differentiation may play a potentially important role in potassium homeostasis in these cells. (bgu.ac.il)
  • Here, we model early human neural tube development using human embryonic stem cells cultured in a microfluidic device. (nature.com)
  • Single-cell transcriptomics revealed that rostro-caudal organization was already established at 24 h of differentiation, and that the first markers of a neural-specific transcription program emerged in the rostral cells at 48 h. (nature.com)
  • Stromal cell CD9 regulates differentiation of hematopoietic stem/progenitor cells. (bdbiosciences.com)
  • The differentiation of mesenchymal stem cells (MSCs) into multiple cell lineages can be exploited as an attractive strategy for cell-based therapy and regenerative medicine [ 1 ]. (hindawi.com)
  • The ECM provides structural and biochemical support to the cells and has various other functions including cell adhesion, cell to cell communication, and differentiation [ 17 , 18 ]. (hindawi.com)
  • Hematopoietic stem and progenitor cells (HPCs) can be maintained in vitro, but the vast majority of their progeny loses stemness during culture. (nih.gov)
  • While murine hematopoiesis has been considered as resolved science, recent results using barcoding, which allows the tracing of single cells in vivo, clearly show that the topology of the murine hematopoietic tree needs revisions. (europa.eu)
  • This knowledge will allow us to test whether cells with different origins have different functions, a key question in immunology in particular during infection and inflammation, and it will help design ways of therapeutically manipulating the hematopoietic system. (europa.eu)
  • FIP1L1-PDGFRA rearrangement has been found in a variety of cell lineages (neutrophils, monocytes, eosinophils, CD34+ cells, mast cells and even lymphoid) consistent with an origin in an hematopoietic stem cells or early progenitors progenitor (Gotlib and Cools, 2008). (atlasgeneticsoncology.org)
  • Melinda is highly proficient in the use of in vitro and in vivo assays for normal and malignant hematopoietic stem and progenitor cells, determination of self-renewal, expansion and lineage specific differentiation capabilities, and the use of induced pluripotent stem cells for the investigation of hematopoietic disorders and leukemic progression and the testing of targeted therapeutics. (edu.au)
  • Granulocyte-macrophage colony-stimulating factor (GM-CSF) promotes the proliferation and differentiation of hematopoietic progenitor cells and the generation of neutrophils, eosinophils, and macrophages. (stemcell.com)
  • In synergy with other cytokines such as stem cell factor, IL-3, erythropoietin, and thrombopoietin, it also stimulates erythroid and megakaryocyte progenitor cells (Barreda et al. (stemcell.com)
  • GM-CSFR is expressed on hematopoietic cells, including progenitor cells and immune cells, as well as non-hematopoietic cells. (stemcell.com)
  • Recombinant human GM-CSF (rhGM-CSF) promotes the production of myeloid cells of the granulocytic (neutrophils, eosinophils and basophils) and monocytic lineages in vivo. (stemcell.com)
  • It has been tested for mobilization of hematopoietic progenitor cells and for treating chemotherapy-induced neutropenia in patients. (stemcell.com)
  • A) The biological activity of Human Recombinant GM-CSF was tested by its ability to promote the proliferation of TF-1 cells. (stemcell.com)
  • MYB is well recognized to be a key regulator of definitive hematopoiesis that plays an important role in the maintenance and multilineage differentiation of hematopoietic stem cells (HSCs). (nih.gov)
  • To explore the role of MYB in human hematopoietic development we have inactivated the gene by bi-allelic TALEN-supported gene targeting in several lines of human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs), and subjected these cells to hematopoietic differentiation in well-defined cell culture conditions. (nih.gov)
  • The gene reporter system showed that MYB is specifically expressed during hematopoietic commitment in the earliest primitive blood cells. (nih.gov)
  • Transposon-mediated rescue of MYB expression in MYB-null cells efficiently restored both the primitive hematopoietic progenitors and immature myeloid cells. (nih.gov)
  • The modified lines including MYB+/+, MYB+/- and MYB-/- were differentiated into hematopoietic cells in our new established serum-free, cytokine-free culture system with minimal BMP4 exposure for mesoderm induction. (nih.gov)
  • Although clonal studies of lineage potential have been extensively applied to organ specific stem and progenitor cells, much less is known about the clonal origins of lineages formed from the germ layers in early embryogenesis. (ca.gov)
  • By applying a quantitative framework to estimate the degree of undersampling we revealed the existence of tripotent mesodermal progenitors derived from pluripotent stem cells, and the subsequent bifurcation of their differentiation into bipotent endothelial/hematopoietic or endothelial/mesenchymal progenitors. (ca.gov)
  • Launch The breakthrough of endothelial progenitor cells, broadly thought as proangiogenic cells (PACs), described a new path in the treatment of cardiovascular disorders (CVDs), since they were recognized as responsible for postnatal vasculogenesis in physiological and regenerative neovascularization after, for example, hind limb ischemia (HLI) (5, 6). (mingsheng88.org)
  • Although low degrees of ROS are necessary for correct function of adult differentiation and cells of progenitors, the circumstances of serious oxidative stress, as an important mechanism root the pathogenesis of CVDs, can lead to PAC harm lowering their antioxidative and proangiogenic features (24, 26, 54). (mingsheng88.org)
  • Technology This ongoing function recognizes a novel, direct function of nuclear aspect E2-related aspect 2 (Nrf2) in angiogenic properties of both bone-marrow-derived proangiogenic progenitor cells and adult endothelial cells. (mingsheng88.org)
  • It is expressed in hematopoietic precursors, endothelial cells, smooth muscle cells and leukocyte subpopulations. (rndsystems.com)
  • Hematopoietic stem cell transplantation (HSCT) involves the intravenous infusion of hematopoietic stem cells in order to reestablish blood cell production in patients whose bone marrow or immune system is damaged or defective. (medscape.com)
  • Several models are proposed to describe the development and differentiation of dendritic cells (DCs). (origene.com)
  • Conventional dendritic cells (cDCs), also known as myeloid dendritic cells or classical dendritic cells, are a type of antigen-presenting cell (APC), cDCs originate from hematopoietic stem cells, specifically from the common myeloid progenitor in the bone marrow. (origene.com)
  • In humans and mice, conventional dendritic cells are divided into two main subtypes: cDC1 and cDC2, each with distinct functions and markers [2]. (origene.com)
  • This study was designed to determine the in vivo performance of three different materials as scaffolds for dental pulp stem cells (DPSC) undergoing induced odontogenic differentiation. (scielo.br)
  • Although different stem cells, morphogens and scaffolds can be combined to simulate natural odontogenic regeneration, it is crucial to determine which options most closely replicate the characteristics of human dental tissues. (scielo.br)
  • They have a higher odontogenic differentiation capacity compared to other cell groups due to their content of specific progenitor cells. (scielo.br)
  • The odontogenic differentiation of human dental pulp stem cells on nanofibrous poly(L-lactic acid) scaffolds in vitro and in vivo. (scielo.br)
  • editors Culture of human stem cells. (scielo.br)
  • We research human embryonic stem (ES) cells, germline stem cells and somatic stem cells. (ncl.ac.uk)
  • We are studying the role that normal chromatin structure plays in gene regulation in hematopoietic cells and how its disruption leads to altered development and cancer. (stanford.edu)
  • This mechanism also applies to hematopoietic cells transformed by other HOX genes, including CDX2, which is highly expressed in a majority of acute myeloid leukemias, thus providing a molecular approach based on GSK-3 inhibitory strategies to target HOX-associated transcription in a broad spectrum of leukemias. (stanford.edu)
  • 2021). The antiandrogen enzalutamide downregulates TMPRSS2 and reduces cellular entry of SARS-CoV-2 in human lung cells . (essex.ac.uk)
  • The inventors have developed a novel system for co-differentiation of hepatocytes and hematopoietic progenitors from human embryonic stem cells (ES cells) that mimics a late fetal liver undergoing hematopoiesis. (warf.org)
  • Small hepatic progenitor cells (SHPCs) derived from these hepatocytes have shown mature metabolic functions as well as engraftment in immunodeficient mice. (warf.org)
  • 1) Hepatocytes generated in this system display late fetal markers (both albumin and alpha-fetoprotein, AFP) and give rise to mature and engraftable small hepatic progenitor cells (SHPCs, which express albumin but not AFP). (warf.org)
  • In this study, we investigated how higher-order chromatin structure modulates differential expression of the human INK4b-ARF-INK4a locus during progenitor cell differentiation, cellular ageing and senescence of cancer cells. (biomedcentral.com)
  • We found that INK4b and INK4a , but not ARF , are upregulated following the differentiation of haematopoietic progenitor cells, in ageing fibroblasts and in senescing malignant rhabdoid tumour cells. (biomedcentral.com)
  • Several HOX transcription factors, including HOXB4, are highly expressed in hematopoietic stem cells (HSCs) but are downregulated upon differentiation towards more mature progeny cells. (dissertations.se)
  • Enforced expression of HOXB4 dramatically increases the regeneration of murine HSC following transplantation, and enhances the repopulating ability of human SCID repopulating cells. (dissertations.se)
  • Adenoviral mediated overexpression of HOXB4 in human primitive hematopoietic cells in vitro generates very high levels of HOXB4 within 24 hours of transduction, leading to an increased differentiation towards myeloid lineages, and to a reduction of primitive hematopoietic progenitors, suggesting that the level of HOXB4 expression may determine the fate options following gene transfer. (dissertations.se)
  • To varying degrees, these fates also extend to the Such state stability is required in stem and progenitor cells to immediate progeny of stem cells, known as progenitor or support self-renewal and maintenance of the uncommitted transit-amplifying cells. (lu.se)
  • A key challenge is to understand how state, but must also afford flexibility in cell-fate choice to permit the different cell-fate options confronting stem and progenitor cell-type diversification and differentiation in response to cells are selected and coordinated such that adoption of a given intrinsic cues or extrinsic signals. (lu.se)
  • Evidence the fate of stem cells has broad ramifications for biomedical suggests that during development or differentiation, cells make science from elucidating the causes of cancer to the use of very precise transitions between apparently stable ``network stem cells in regenerative medicine. (lu.se)
  • derivation of this specialized endothelium Ezetimibe enzyme inhibitor from human being embryonic stem cells (hESCs) provides an priceless platform to study and dissect blood specification and the emergence of hematopoietic stem and progenitor cells. (bioskinrevive.com)
  • To date, a great deal of data describing the emergence of blood cells from human being HE have been acquired using stromal co-culture protocols Ezetimibe enzyme inhibitor (Choi et?al. (bioskinrevive.com)
  • 2012). At later EB stage, most CD43+ cells upregulated the manifestation of CD41a and CD235a, and were enriched for megakaryocyte and erythroid progenitors, respectively (Klimchenko et?al. (bioskinrevive.com)
  • Ineffective hematopoiesis in cases of myelodysplastic syndromes - the role of CD34+ stem and progenitor cells. (bsb-bg.eu)
  • Human myelopoiesis is an exciting biological model for cellular differentiation since it represents a plastic process where multipotent stem cells gradually limit their differentiation potential, generating different precursor cells which finally evolve into distinct terminally differentiated cells. (biomedcentral.com)
  • Differentiation of human pluripotent stem cells (hPSCs) into functional cell types is a crucial step in cell therapy. (ashpublications.org)
  • Human embryonic stem cells (hESCs) derived from an early embryo can self-renew indefinitely and differentiate into a variety of cell types. (ashpublications.org)
  • The key difference between hematopoietic stem cells and progenitor cells is that hematopoietic stem cells have the ability to differentiate into different types of cells while progenitor cells are more specific and they differentiate into target cells. (differencebetween.com)
  • Stem cells have the ability to change into many different types of cells and grow definitely in the human body. (differencebetween.com)
  • Hematopoietic stem cells are immature cells that develop into all types of blood cells. (differencebetween.com)
  • Progenitor cells are descendants of stem cells that further differentiate into specialized cell types. (differencebetween.com)
  • Hematopoietic stem cells (HSCs) are immature cells found in the peripheral blood and bone marrow. (differencebetween.com)
  • Progenitor cells are the cells that originate from stem cells and further differentiate to create specialized cell types. (differencebetween.com)
  • Progenitor cells are capable of differentiating into cells that belong to the same tissue or organ of each progenitor cell. (differencebetween.com)
  • Progenitor cells are an intermediary step in the development of mature cells in tissues, organs, blood, and the central nervous system. (differencebetween.com)
  • In the central nervous system of humans, there are three types of fully differentiated cells known as neurons, astrocytes, and oligodendrocytes. (differencebetween.com)
  • These cells evolve from the differentiation of neural progenitor cells (NPCs). (differencebetween.com)
  • Hematopoietic progenitor cells (HPCs) are also intermediates in the development of blood cells. (differencebetween.com)
  • Hematopoietic stem cells differentiate into multipotent progenitor cells. (differencebetween.com)
  • These multipotent progenitor cells differentiate into either common myeloid progenitor (CMP) or common lymphoid progenitor cells (CLP). (differencebetween.com)
  • Both CMPs and CLPs are types of oligopotent progenitor cells. (differencebetween.com)
  • The primary function of progenitor cells is to replace damaged cells. (differencebetween.com)
  • Therefore, progenitor cells are necessary for repairs and to maintain tissues after an injury. (differencebetween.com)
  • On the other hand, progenitor cells are more specific than hematopoietic stem cells and can differentiate into target cells. (differencebetween.com)
  • Cre ;Ai9 line that uniformly marks interneuron progenitors, We therefore collected a set of 15,522 single cells across a developmental timecourse (E13.5, E18, P10), obtaining 3,432 additional P56 cells from a public resource hosted by the Allen Brain Atlas. (authorea.com)
  • Disruption of murine leads to embryonic lethality seen as a a major lack of bloodstream cells and reductions in hematopoietic precursors (22, 23). (researchhunt.com)
  • Langerhans cell histiocytosis (LCH) is a group of idiopathic disorders characterized by the presence of cells with characteristics similar to bone marrow-derived Langerhans cells juxtaposed against a backdrop of hematopoietic cells, including T-cells, macrophages, and eosinophils. (medscape.com)
  • Many existing protocols for neuronal differentiation of human pluripotent cells result in heterogeneous cell populations and unsynchronized differentiation, necessitating the development of methods for labeling specific cell populations. (lu.se)
  • This strategy provides a useful tool for visualization and identification of neural progeny derived from human pluripotent stem cells. (lu.se)
  • We provide detailed protocols for lentiviral transduction, neural differentiation, and subsequent analysis of human embryonic stem cells. (lu.se)
  • Given this, human pluripotent stem cells, such as human embryonic stem cells (hESCs) and induced pluripo- tent stem cells (hiPSCs), have become a unique cell source for study- ing early human brain development. (lu.se)
  • However, current protocols for deriving neurons from human pluripotent stem cells give rise to heterogeneous cell populations both in regard to the temporal aspects and the cellular composition. (lu.se)
  • Although possible, these strategies are often complicated to transfer to human cells due to technical issues (9 ), and only a few successful cases have been described ( 10, 11 ). (lu.se)
  • Here we describe how this system can be used for human embry- onic stem cells. (lu.se)
  • Association of stromal cell-derived factor-1-3'A polymorphism to higher mobilization of hematopoietic stem cells CD34+ in Tunisian population. (cdc.gov)
  • At these sites, which are a compound of stromal cells, extracellular matrix and soluble factors, complex molecular interactions that maintain the essential properties of stem cells occur, such as self-renewal and differentiation into multiple lineages, according to the organism's needs. (bvsalud.org)
  • Likewise, these cells give rise to progenitor cells committed to a particular cell lineage, and play a crucial role in tissue repair and homeostasis. (bvsalud.org)
  • ABSTRACT This study examined haematopoietic stem cells of 19 high-risk cases of myelodysplastic syndrome (MDS) for apoptotic and anti-apoptotic signals and cellular proliferation and correlated these with clinical and cytogenetic subtypes, particularly trisomy 8. (who.int)
  • The essence of MDS is damage of In the current work we examined All our patients were of the high-risk colony-forming units [4], but the defect haematopoietic stem cells of high-risk group and none of them was eligible of the haematopoietic stem cells is not MDS cases for apoptotic and anti-apop- for stem cell transplantation. (who.int)
  • Our research focuses on how hematopoietic stem cells (HSCs) and their immediate offspring are regulated in situations such as infections, stress, aging and leukemia. (lu.se)
  • We applied lentiviral tagging followed by vector integration site analysis (VISA) with high-throughput sequencing to investigate the ontogeny of the hematopoietic, endothelial and mesenchymal lineages as they emerge from human embryonic mesoderm. (ca.gov)
  • We discuss these properties with examples both from the hematopoietic and embryonic stem cell (ESC) systems. (lu.se)
  • The study of brain development in humans is limited by the lack of tissue samples and suitable in vitro models. (nature.com)
  • We have developed and applied FACS-based high-resolution cell fractionation procedures to map the differentiation processes from HSCs. (lu.se)
  • A broader interest of our laboratory is to understand the molecular regulation of HSCs both from the perspectives of self-renewal and differentiation, and how these features become altered upon disease-causing or disease-associated perturbations. (lu.se)
  • According to the classical model, it proposes a common myeloid progenitor (CMP) as the precursor of both monocytes/macrophages and DCs. (origene.com)
  • By studying murine transgenic leukemia models developed in our laboratory, we are currently exploring the relationships between hematopoietic progenitor cell identities and the development and progression of acute leukemia. (lu.se)
  • In parallel, human hematopoiesis has been overlooked and is widely considered as following the same tree as in mice. (europa.eu)
  • To leverage our understanding of normal human hematopoiesis, tools for barcoding in humans are urgently needed. (europa.eu)
  • With this method, we will provide the first in vivo description of the human hematopoietic tree and identify new progenitors and key molecular factors involved in human hematopoiesis. (europa.eu)
  • More generally it will impact our understanding of stem cell differentiation for which hematopoiesis is used as an exemplar. (europa.eu)
  • Melinda Tursky has specialised in the field of normal and malignant human hematopoiesis and has a strong translational interest in the pathogenesis of hematological cancers. (edu.au)
  • Our data indicate that in contrast to its previously attributed exclusive role in definitive hematopoiesis, MYB is indispensable for primitive human hematopoiesis. (nih.gov)
  • The differentiated hematopoietic precursors express mature markers that show promise for engraftment (study in progress). (warf.org)
  • We found that MYB was not required for initial hematopoietic commitment of nascent mesoderm and emergence of primitive, yolk sac-type human hematopoietic progenitors. (nih.gov)
  • Genetic Tagging During Human Mesoderm Differentiation Reveals Tripotent Lateral Plate Mesodermal Progenitors. (ca.gov)
  • Our data set up that hemogenic endothelium populations endowed with primitive and definitive hematopoietic potential are specified simultaneously from your mesoderm in differentiating hESCs. (bioskinrevive.com)
  • To time, it really is still as yet not known whether HE subsets with different hematopoietic potentials emerge in successive waves during hESC differentiation, whether HE populations are preserved inside the differentiating lifestyle as time passes, or whether one exclusive population of He's produced early from mesoderm and steadily differentiates inside the lifestyle. (bioskinrevive.com)
  • Stem and progenitor cell populations are often heterogeneous, which may reflect stem cell subsets that express subtly different properties, including different propensities for lineage selection upon differentiation, yet remain able to interconvert. (lu.se)
  • Following hemogenic potential of endothelium cell populations frequently during the period of hESC differentiation would address a few of these problems but to time this has hardly ever been reported. (bioskinrevive.com)
  • 2006). In those ethnicities, different hematopoietic populations emerged from CD144+CD31+CD73? (bioskinrevive.com)
  • Differentiation between African populations is evidenced by the diversity of alleles and haplotypes of HLA class I loci. (cdc.gov)
  • IL-5 has been shown to promote the growth of immature hematopoietic BFU-E progenitors, stimulate the activation and differentiation of eosinophils, and promote the generation of cytotoxic lymphocytes. (biolegend.com)
  • Peripheral blood contamination in human bone marrow aspirates was calculated from the ratios between the erythrocyte and nucleated cell counts in both the bone marrow aspirate and a simultaneously obtained peripheral blood sample. (karger.com)
  • Following the action of cytokines and growth factors, such as vascular endothelial growth factor (VEGF) and stromal cell-derived factor-1 (SDF-1), progenitors of bone marrow (BM) origin circulate in peripheral blood (PB) and might contribute to the formation Hycamtin novel inhibtior of blood vessels in damaged/ischemic tissue (7, 10, 46). (mingsheng88.org)
  • MSCs can easily be obtained from various human tissue sources such as the bone marrow, cord blood, placenta, and adipose [ 2 - 5 ]. (hindawi.com)
  • An odontogenic medium modified by the addition of recombinant human bone morphogenetic protein 2 was used in the experimental groups to induce differentiation. (scielo.br)
  • Bone morphogenetic proteins (BMP), known as active components of odontogenic differentiation and tooth development, have been used as a growth factor in an appropriate medium. (scielo.br)
  • Decreased WBC count, leukopenia, is seen when supply is depleted by infection or treatment such as chemotherapy or radiation therapy, or when a hematopoietic stem cell abnormality does not allow normal growth/maturation within the bone marrow, such as myelodysplastic syndrome or leukemia. (medscape.com)
  • While our initial manuscript focused on our unexpected finding that committed progenitor subsets could not be identified in mitotic progenitors, all reviewers requested further analysis of when distinct interneuron subsets first emerge during development, and how heterogeneity in the eminences could be connected to later timepoints. (authorea.com)
  • Four sequence-related potassium channel genes - shaker, shaw, shab, and shal - have been identified in Drosophila, and each has been shown to have human homolog(s). (ucsc.edu)
  • In addition, their osteogenic differentiation potential was increased, and genes involved in cell adhesion, FGF-2 signalling, cell cycle, stemness, cell differentiation, and cell proliferation were upregulated, compared to that of the MSCs cultured on uncoated plates. (hindawi.com)
  • The inventors have previously shown that such SHPCs express high levels of mature hepatic genes, perform xenobiotic metabolism at rates equal to primary human hepatocytes, and support engraftment in immunodeficient mice (P180325). (warf.org)
  • Gene expression data from 24 experiments for 8 different cell types of the human myelopoietic lineage were used to generate an integrated myelopoiesis dataset of 9,425 genes, each reliably associated to a unique genomic position and chromosomal coordinate. (biomedcentral.com)
  • The analysis of genomic expression during human myelopoiesis using an integrative computational approach allowed discovering important relationships between genomic position, biological function and expression patterns and highlighting chromatin domains, including genes with coordinated expression and lineage-specific functions. (biomedcentral.com)
  • The analysis of transcriptome data in the perspective of the genomic organization of genes could certainly shed light not only on the aberrations leading to pathological states but also on the physiological mechanisms of all cellular processes, including cellular differentiation. (biomedcentral.com)
  • In parallel, we used a version of the SMART-Seq2 protocol to obtain deep sequencing data (4-7,000 genes/cell) from 400 mitotic progenitors. (authorea.com)
  • Finally, we added data produced using the 10X genomics system for 7,000 postmitotic progenitors with significantly increased sequencing depth (1,500-11,000 UMI/cell, 1,000-4,000 genes/cell), and observed identical branching patterns as in our original Drop-seq dataset. (authorea.com)
  • At a very basic level, we are learning who likes to work with whom to regulate around 20,000 human genes," said Michael Snyder , PhD, professor and chair of genetics at Stanford. (pharmaceuticalintelligence.com)
  • NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. (cusabio.com)
  • Additionally, Abedin and King showed that FGF-2 suppresses the cellular senescence of human MSCs [ 17 ]. (hindawi.com)
  • In contrast to studies that have used VISA to track differentiation of self-renewing stem cell clones that amplify significantly over time, we focused on a population of progenitor clones with limited self-renewal capability. (ca.gov)
  • It also functions as an L-Selectin ligand and may be involved in the inhibition of stem cell differentiation and leukocyte-endothelial adhesion (1, 2). (rndsystems.com)
  • Donor CTLA-4 genotype influences clinical outcome after T cell-depleted allogeneic hematopoietic stem cell transplantation from HLA-identical sibling donors. (cdc.gov)
  • 2013). The performance of hematopoietic differentiation differs between your two methodologies because of parameters such as for example serum, stromal maintenance, or EB size, amongst others elements (Kardel and Eaves, 2012, Vodyanik et?al. (bioskinrevive.com)
  • Venus gene reporter was inserted into the knock-in allele to monitor MYB expression during the course of the hESC/iPSC differentiation. (nih.gov)
  • Our research focuses on developmental pathways that regulate hematopoietic cell growth and differentiation and are disrupted in the course of neoplastic transformation, particularly in leukemias and lymphomas. (stanford.edu)
  • This Notch ligand was also proven to regulate the hematopoietic destiny of individual hemato-endothelial progenitors (Ayllon et?al. (bioskinrevive.com)
  • However, inactivation of MYB severely abrogated proliferation of the primitive erythroid and mixed erythroid-macrophage-megakaryocyte progenitors. (nih.gov)
  • The clinical application of MSCs to tissue engineering has been introduced due to their numerous advantages including high expansion potential and extensive differentiation potential [ 6 , 7 ]. (hindawi.com)
  • The aryl hydrocarbon receptor directs hematopoietic progenitor cell expansion and differentiation. (umassmed.edu)
  • When used without hematopoietic growth factor in children, ATG and cyclosporine-based immunosuppressive therapy has been shown to lead to an excellent response and survival rate with low incidence of clonal evolution. (medscape.com)
  • Consequently, investigation of molecular heterogeneity in the differentiation potential of HSPC is vital to identify novel regulators that affect generation of specific cell types, especially RBC. (lu.se)
  • The findings are of interest in understanding molecular processes involved in regulating erythropoiesis, as well as the potential to develop host-directed therapies for malarial parasites that target human reticulocytes. (elifesciences.org)
  • Therefore, we will provide insights into both cell lineage and the molecular mechanisms involved in differentiation. (europa.eu)
  • Monomeric human IL-5 is a 126 amino acid protein with a reported molecular weight of 26 kD for the homodimeric protein. (biolegend.com)
  • The Regenerative Medicine (RM) field requires a new type of researcher, well-trained in fundamental RM concepts and methods, able to integrate human biology and engineered systems with awareness of challenges involved in advancing basic research, regulatory science, and clinical translation. (wakehealth.edu)
  • In the last decade, there has been an increased desire for the characterization of this precursor from differentiating hESCs using several methods, generally through three-dimensional embryoid body (EB) differentiation (Ditadi et?al. (bioskinrevive.com)
  • This research program seeks to meet this need by taking advantage of recent developments in next generation sequencing to provide an improved barcoding method for in vivo use in human. (europa.eu)
  • It suggests that DCs arise from a common DC progenitor (CDP), which gives rise to pre-DCs that subsequently differentiate into conventional DCs (cDCs) and plasmacytoid DCs (pDCs). (origene.com)
  • 2006). Using EB differentiation protocols, the onset of hematopoietic commitment was also defined from the manifestation of CD43, growing from a CD34+ endothelial precursor human population (Kennedy et?al. (bioskinrevive.com)
  • We conduct studies of early human brain development. (ncl.ac.uk)
  • They play a role in cell development and differentiation. (differencebetween.com)
  • The development of the human blood-CSF-brain barrier. (cdc.gov)
  • Moreover, the level of MYB expression was highest at the commitment stage of differentiation and significantly decreased at the maturations stage. (nih.gov)
  • In humans, pDCs can be identified by the expression of several specific cell surface proteins, including CD123 (the IL-3 receptor alpha chain), BDCA-2, and BDCA-4. (origene.com)
  • This identified IOX1, a pan-histone demethylase inhibitor, which selectively downregulates α-globin expression without perturbing erythroid differentiation or general gene expression, more specifically β-like globin expression. (haematologica.org)
  • Developmentally regulated EZH2 levels are one of the factors that can determine the higher order chromatin structure and expression pattern of the INK4b-ARF-INK4a locus, coupling human progenitor cell differentiation to proliferation control. (biomedcentral.com)
  • Expression of the INK4b-ARF-INKa locus during cellular ageing and differentiation . (biomedcentral.com)
  • This study aimed at investigating the genomic expression during myeloid differentiation through a computational approach that integrates gene expression profiles with functional information and genome organization. (biomedcentral.com)
  • We will apply these approaches to fully integrate seven human neuronal scRNA-seq datasets, combining data from shallow, deep, cytoplasmic, and nuclear scRNA-seq technologies. (authorea.com)