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  • endothelial
  • Encouragingly, these findings aided the in vivo identification of a mouse cell population expressing endothelial and early hematopoietic markers in the mouse placenta , suggesting that the reprogramming of mouse fibroblasts to hemogenic precursor cells recapitulates developmental hematopoiesis. (stemcellsportal.com)
  • The team now returns with a Cell Reports study in which they shift their focus from the specification of mouse to human hematopoietic stem cells and describe the generation of hemogenic precursors from human fibroblasts via an endothelial intermediate and highlight the general importance of transcription factor cooperation during human hematopoietic stem cell specification . (stemcellsportal.com)
  • The authors discovered that human fibroblasts, in a similar manner to mouse fibroblasts, underwent reprogramming through an intermediate endothelial stage (as marked by the expression of the PPARG, VWF, and FOXC2 angiogenesis-associated genes) to a hemogenic precursor cell fate following the forced expression of the GATA2, GFI1B, and FOS transcription factors. (stemcellsportal.com)
  • During mechanistic studies, the authors confirmed that collaborative interactions between the three transcription factors initiated the silencing of fibroblastic gene expression and then activated endothelial and hematopoietic gene expression. (stemcellsportal.com)
  • HSPCs
  • In order to elucidate the molecular mechanisms for TE up-regulation after senescence of HSPCs, we employed whole-genome bisulfite sequencing in paired senescent and active human HSPCs in vivo from healthy subjects. (springer.com)
  • This is the first report characterizing the methylome of senescent human HSPCs. (springer.com)
  • We wanted to examine whether the TE induction observed in human senescent HSPCs in vivo was due to a similar loss of DNA methylation. (springer.com)
  • For this, we performed whole-genome bisulfite sequencing (WGBS) on senescent and active human HSPCs in vivo and characterized their methylome. (springer.com)
  • transcriptional
  • Together, our findings indicate that, through interaction with ATF2 factors, TCF1/LEF1 promote the growth of hematopoietic malignancies in the absence of β-catenin stabilization, thus establishing a new mechanism for TCF1/LEF1 transcriptional activity distinct from that associated with canonical Wnt signaling. (prolekare.cz)
  • immune
  • These studies present a potential therapeutic approach and an important tool to understand better and to optimize the human immune response to melanoma and, potentially, to other types of cancer. (pnas.org)
  • 3 A dose of ionizing radiation above 1 Gy in humans poses a risk of injury to the bone marrow and hematopoietic system, which leads to long-term compromised immune function and increased susceptibility to infection and internal and external hemorrhage. (haematologica.org)
  • vivo
  • Here, we present the generation of functional tumor-specific human T cells in vivo from genetically modified human hematopoietic stem cells (hHSC) using a human/mouse chimera model. (pnas.org)
  • Following tumor challenge, these transgenic CD8 + T cells, in the absence of additional manipulation, limited and cleared human melanoma tumors in vivo. (pnas.org)
  • It has been well characterized in cell lines, but there is currently very limited data available on human senescence in vivo. (springer.com)
  • However, comprehensive molecular features of human senescent cells in vivo have not been well investigated. (springer.com)
  • Most importantly, in the future, RDTR/SCF-LVs might completely obviate ex vivo handling and simplify gene therapy for many hematopoietic defects because of their applicability to direct in vivo inoculation. (epfl.ch)
  • expression
  • Further, expression of TCF1 and LEF1, but not TCF4, stimulated TCF/LEF reporter activity in certain human cell lines independently of β-catenin. (prolekare.cz)
  • Our results suggest that inhibitory cytokines produced by the bone marrow microenvironment are likely to be involved in the regulation of MIP-1 receptor expression on hematopoietic cells. (bloodjournal.org)
  • mouse
  • Conclusions Our data indicate that δ-tocotrienol protects mouse bone marrow and human CD34 + cells from radiation-induced damage through extracellular signal-related kinase activation-associated mammalian target of rapamycin survival pathways. (haematologica.org)
  • sources
  • Delivery of ZFNs and donor templates results in high levels of gene correction in human CD34+ cells from multiple sources, including SCD BM. (bloodjournal.org)
  • early
  • Recurrent mutations in cohesin complex proteins have been identified in pre-leukemic hematopoietic stem cells and during the early development of acute myeloid leukemia and other myeloid malignancies. (nih.gov)