Human glucose transporter glut3. Medical search. Frequent questions

Glucose is fundamental to the metabolism of mammalian cells. (thermofisher.com)
Glucose is removed from ASL in proximal airways via facilitative glucose transporters, down a concentration gradient generated by intracellular glucose metabolism. (ersjournals.com)
Glucose removal is determined by cellular glucose uptake and metabolism ( fig. 1 ) [ 4 , 12 - 14 ]. (ersjournals.com)
These phase contrast and confocal immunofluorescence images (inset) show 3-D cultures of non-malignant (S1) and malignant (T4-2) human breast cells in which glucose metabolism is inhibited by the addition of 2-deoxy-D-glucose (2DG). (rdworldonline.com)
In our 3-D culture assay, glucose uptake and metabolism determined the signaling activity and the morphology of both malignant and non-malignant mammary epithelial cells," Bissell says. (rdworldonline.com)
Our ability to revert the malignant phenotype in our 3-D cultures shed new light on the importance of glucose uptake and metabolism in inducing oncogenic signaling. (rdworldonline.com)
The researchers cautioned that it is not yet completely clear whether abnormalities in brain glucose metabolism are definitively linked to the severity of Alzheimer's disease symptoms or the speed of disease progression. (nih.gov)
Although the adult human brain represents only 2% of the total body weight, it consumes up to 20% of the total glucose metabolism under normal resting physiological conditions (e.g. (frontiersin.org)
Glucose is the major substrate for ATP synthesis through glycolysis and oxidative phosphorylation (OXPHOS), whereas intermediary metabolism through the tricarboxylic acid (TCA) cycle utilizes non-glucose-derived monocarboxylates, amino acids, and alpha ketoacids to support mitochondrial ATP and GTP synthesis. (molvis.org)
Confocal studies showed differential cellular and compartmental distribution of isozymes involved in glucose, glutamate, glutamine, lactate, and creatine metabolism. (molvis.org)
These findings indicate that similarities with erythropoietin regulation extend to the oxygen-dependent regulation of genes encoding glucose transporters and glycolytic enzymes but not to the regulation of mitochondrial transcripts, and they show that in glucose metabolism regulation by this system is isoenzyme- or isoform-specific. (ox.ac.uk)
This review will discuss the metabolic reprogramming of cancer, possible explanations for the high glucose consumption in cancer cells observed by Warburg, and suggest key experimental practices we should consider when studying the metabolism of cancer. (researchgate.net)

Both neurons and astrocytes take up glucose via the cell-specific transporters GLUT1 and GLUT3. (epilepsygenetics.net)

Several glucose transporter protein (Glut) isoforms have been identified and shown to function in response to insulin and IGF1 induced signaling. (thermofisher.com)
These functional differences allow members of the Glut family to finely regulate whole-body glucose homeostasis. (rndsystems.com)
Since the mid-1960s, it has been known that there are energy-dependent, sodium-coupled glucose transporter (SGLT) and energy-independent, facilitative glucose transporter (GLUT) pathways for glucose uptake in the lung [ 1 ], and that glucose can permeate the alveolar epithelial barrier [ 2 ]. (ersjournals.com)
Acetyl-L-carnitine (ALC), a neuroprotective agent, suppresses the effects of alcohol on glucose uptake and GLUT levels, thus reducing neurotoxicity and neuronal degeneration. (njit.edu)
Solute carrier family 2, facilitated glucose transporter member 3 (Glucose transporter type 3, brain) (GLUT-3 or GLUT3). (lbl.gov)

Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Glucose Transporter 3 (GLUT3) in tissue homogenates, cell lysates and other biological fluids. (bioemm.com)
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Glucose Transporter 3 (GLUT3) in samples from tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species. (bioemm.com)
The following product was used in this experiment: GLUT3 Polyclonal Antibody from Thermo Fisher Scientific, catalog # 20403-1-AP. (thermofisher.com)
This antibody was generated against the internal region of human GLUT3 and may cross-react with GLUT14.20403-1-AP antibody detects the bands around 48-60 kDa in SDS-PAGE. (thermofisher.com)
Western Blot: Glucose Transporter GLUT8 Antibody [NBP1-59812] - Human Fetal Heart, Antibody Dilution: 1.0 ug/ml. (novusbio.com)
Western Blot: Glucose Transporter GLUT8 Antibody [NBP1-59812] - NCI-H226 cell lysate, concentration 0.2-1 ug/ml. (novusbio.com)
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Major Histocompatibility Complex Class I G (MHCG) in serum, plasma, tissue homogenates and other biological fluids. (agctsequencing.com)
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Major Histocompatibility Complex Class I G (MHCG) in samples from serum, plasma, tissue homogenates and other biological fluids with no significant corss-reactivity with analogues from other species. (agctsequencing.com)
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Inhibin Beta B (INHbB) in samples from Serum, plasma, tissue homogenates and other biological fluids. (bestpractice-life.pl)
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Juxtaposed With Another Zinc Finger Protein 1 (JAZF1) in Tissue homogenates, cell lysates and other biological fluids. (worldcarecouncil.org)

Working with Bissell, Yasuhito Onodera, a Japanese postdoctoral fellow in her research group who is now an asst. prof. in Japan, examined the expression of glucose transporter proteins in human breast cells. (rdworldonline.com)

Gene expression profiling by qRT-PCR, confirmed that FNC-B4 cells express GnRH and several genes relevant for GnRH neuron function (KISS1R, KISS1, sex steroid and leptin receptors, FGFR1, neuropilin 2, and semaphorins), along with glucose transporters (GLUT1, GLUT3, and GLUT4). (ironmagazine.com)
130 fois plus sélectif pour GLUT1 que GLUT2, GLUT3 et GLUT4. (medchemexpress.com)
BAY-876 ist >130-mal selektiver für GLUT1 als GLUT2, GLUT3 und GLUT4. (medchemexpress.com)
BAY-876 is >130-fold more selective for GLUT1 than GLUT2 , GLUT3 , and GLUT4 . (medchemexpress.com)

RhoA activation triggers a glycolytic burst through the translocation of the glucose transporter SLC2A3/GLUT3 to fuel the cellular contractile machinery, as demonstrated across multiple endothelial cell types. (nature.com)
Fig. 5: RhoA induces glucose transporter SLC2A3 translocation which drives subcellular glycolysis in thrombin-stimulated contractile endothelial cells. (nature.com)

SLC2A8 is the insulin-regulated facilitative glucose transporter.SLC2A8 binds cytochalasin B in a glucose-inhibitable manner.SLC2A8 seems to be a dual-specific sugar transporter as it is inhibitable by fructose. (novusbio.com)

You need info about Human Glucose transporter 3 (GLUT3) ELISA Kit or any other Gentaur produtct? (gentaurshop.com)
Description: A sandwich ELISA kit for detection of Glucose Transporter 3 from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids. (bioemm.com)
The Elisa Gluten Test reagent is RUO (Research Use Only) to test human serum or cell culture lab samples. (mygentaur.com)
Description: A competitive ELISA for quantitative measurement of Human oxyntomodulin (OXM) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. (tuberculosisdrugresearch.org)
Description: A sandwich ELISA kit for detection of Inhibin Beta B from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids. (bestpractice-life.pl)
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids. (bestpractice-life.pl)
Description: Quantitativecompetitive ELISA kit for measuring Human inhibin beta-B, Inhbb in samples from serum, plasma. (bestpractice-life.pl)
Description: A sandwich ELISA kit for detection of Juxtaposed With Another Zinc Finger Protein 1 from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids. (worldcarecouncil.org)

EtOH may also have a direct effect on glucose uptake in neurons and astrocytes, which has not been previously described. (njit.edu)

Facilitative simple sugar uptake by mammalian cells is mediated by a family of glucose transporters. (rndsystems.com)

Next, they used proteomics - the large-scale measurement of cellular proteins - to tally levels of GLUT3, a glucose transporter protein, in neurons. (nih.gov)

The blastocyst/testis glucose transporter, Glut8 (Doege et al. (lbl.gov)

However, much subsequent research in this area was focused on the role of pulmonary SGLT transport as a modifier of lung liquid volume [ 3 , 4 ], and the effects of starvation and diabetes on glucose transport [ 1 , 5 ]. (ersjournals.com)

Placentas exhibit signs of adaptation to stress, including larger maternal blood spaces and increased expression of nutrient transporter genes. (elifesciences.org)
In human solid glioblastoma we have observed a coordinated overexpression of a panel of pro-inflammatory genes as compared to host normal tissue. (biomedcentral.com)

If your glucose and insulin regulation is working ok your glucose levels won't reach the high levels the Italians used in their experiment. (ironmagazine.com)
The Ang II action on insulin signalling results in alteration of insulin-mediated glucose transport. (easdec.org)
While similarities between diabetes and Alzheimer's have long been suspected, they have been difficult to evaluate, since insulin is not needed for glucose to enter the brain or to get into neurons. (nih.gov)

Glucose transporters (GLUTs) and sodium-glucose co-transporters (SGLTs) are overexpressed in multiple malignancies, and are correlated with treatment resistance, clinical factors, and poor overall survival (OS). (nih.gov)

Understanding of mechanisms underlying lung glucose homeostasis could identify new therapeutic targets for control of ASL glucose and prevention and treatment of lung infection. (ersjournals.com)
ERS pathways are triggered by the unbalance of ER environment, including hypoxia, disturbance of Ca 2+ homeostasis and glucose starvation. (springer.com)

The figures below show that high concentrations of glucose reduce the release of GnRH. (ironmagazine.com)
The figure below shows that high concentrations of glucose inhibited the production of GnRH through leptin. (ironmagazine.com)
To date, little is known about the direct effects of pathological high glucose concentrations on human GnRH neurons. (ironmagazine.com)
Consistent with previous studies, leptin treatment significantly induced GnRH mRNA expression at 5?mM glucose, but not in the presence of high glucose concentrations. (ironmagazine.com)
Here, we consider a newly identified role for pulmonary glucose transport in maintaining low airway surface liquid (ASL) glucose concentrations and propose that this contributes to lung defence against infection. (ersjournals.com)
These processes vary between species but universally maintain ASL glucose at 3-20-fold lower concentrations than plasma. (ersjournals.com)
ASL glucose concentrations are increased in respiratory disease and by hyperglycaemia. (ersjournals.com)
This is in contrast to conditions in the gut and kidney, where luminal glucose concentrations regularly exceed plasma glucose concentrations [ 6 , 7 ]. (ersjournals.com)
ASL glucose concentrations are the net result of diffusion of glucose from blood and interstitial fluid across the respiratory epithelium into the ASL, and removal of glucose from ASL by epithelial glucose transport processes. (ersjournals.com)
Current model of the mechanisms controlling glucose concentrations in the surface liquid lining the airway and distal lung epithelium. (ersjournals.com)
The focus was on the glucose transporter known as GLUT3, the concentrations of which Onodera and Bissell showed are 400 times greater in malignant than in non-malignant breast cells. (rdworldonline.com)

The study was carried out using a 3-D culture assay developed earlier by Bissell and her group for mouse mammary cells and later with her collaborator, Ole Petersen, for human breast cells. (rdworldonline.com)
Bissell says this demonstration of an active role in breast cancer development for glucose uptake could only have been revealed through a 3-D culture assay in which both malignant and non-malignant breast cells behave in a manner that is phenotypically analogous to their corresponding architecture in living tissue. (rdworldonline.com)
Description: Enzyme-linked immunosorbent assay based on the Competitive Inhibition method for detection of Human Oxyntomodulin (OXM) in samples from Serum, plasma and other biological fluids with no significant corss-reactivity with analogues from other species. (tuberculosisdrugresearch.org)

Bissell would go on to discover that the cause of increased aerobic glycolysis was a dramatic increase in glucose uptake by cancer cells, but at that time did not determine whether this increase was the cause of malignant transformation. (rdworldonline.com)
They found distinct abnormalities in glycolysis, the main process by which the brain breaks down glucose, with evidence linking the severity of the abnormalities to the severity of Alzheimer's pathology. (nih.gov)
Lower rates of glycolysis and higher brain glucose levels correlated to more severe plaques and tangles found in the brains of people with the disease. (nih.gov)
The team tracked the brain's usage of glucose by measuring ratios of the amino acids serine, glycine and alanine to glucose, allowing them to assess rates of the key steps of glycolysis. (nih.gov)

Upon increased demand neurons ability to take up glucose is limited and lactate provided by astrocytes becomes the primary oxidative fuel. (epilepsygenetics.net)
They observed high glucose consumption and large amounts of lactate excretion from cancer cells compared with normal cells, which oxidised glucose using mitochondria. (researchgate.net)
glucose is converted into lactate. (researchgate.net)

Even if obtained in vitro, our findings support the idea of a deleterious direct contribution of hyperglycemia on human GnRH neurons, thus providing new insights into the pathogenic mechanisms linking hypogonadotropic hypogonadism to metabolic disorders", the researchers write. (ironmagazine.com)
In conclusion, our findings demonstrate a deleterious direct contribution of high glucose on human GnRH neurons, thus providing new insights into pathogenic mechanisms linking metabolic disorders to reproductive dysfunctions. (unifi.it)

High abundance of glucose in the cytoplasm of a cancer cell also increases flux into other metabolic pathways such as hexosamine biosynthetic pathway (HBP) [ 7 ]. (degruyter.com)
Glucose diffuses into ASL via paracellular pathways at a rate determined by paracellular permeability and the transepithelial glucose gradient. (ersjournals.com)
Furthermore, through a series of painstaking analysis, we have discovered two new pathways through which increased uptake of glucose could itself activate other oncogenic pathways. (rdworldonline.com)
We found that overexpression of GLUT3 in the non-malignant human breast cells activated known oncogenic signaling pathways and led to the loss of tissue polarity and the onset of cancerous growth," Bissell says. (rdworldonline.com)
Conversely, the reduction of GLUT3 in the malignant cells led to a phenotypic reversion, in which the oncogenic signaling pathways were suppressed and the cells behaved as if they were non-malignant even though they still contained the malignant genome. (rdworldonline.com)

HIF-1α is required for disturbed flow-induced metabolic reprogramming in human and porcine vascular endothelium. (nature.com)
Cancer cells also enhance glucose-uptake to fuel these enhanced metabolic activities [ 2 ]. (degruyter.com)
Among metabolic factors hyperglycemia has been implicated in the control of the reproductive axis at central level, both in humans and in animal models. (ironmagazine.com)
Cerebral function requires the cooperative interaction between different cell types, namely neurons, astrocytes, microglia and oligodendrocytes, and depends on high metabolic activity supported by continuous supply of oxygen and glucose from the blood ( Siesjö, 1978 ). (frontiersin.org)
Blood flow is indeed directly related to the cerebral metabolic rate of glucose consumption (CMR glc ) ( Sokoloff, 1978 ). (frontiersin.org)

The erythrocyte/brain hexose facilitator, glucose transporter-1, Gtr1 or Glut1. (lbl.gov)

GLUT3 is detectable in a few normal cell type spermatids in testis with active spermatogenesis, placental trophoblast membranes, and neurons in brain. (thermofisher.com)
GLUT3 staining is also detectable in human cancers including those of the ovary, lung, and testis. (thermofisher.com)

For over 50 years, glucose has been recognised to cross the lung epithelial barrier and be transported by lung epithelial cells. (ersjournals.com)
In primary cultures of human bronchial epithelial cells (HBECs) with 16.6±0.4 mM glucose in the basolateral medium, ASL glucose was 2.2±0.5 mM [ 11 ]. (ersjournals.com)
KLK5 is secreted by human bronchial epithelial cells and elevated in asthma bronchial alveolar lavage. (bvsalud.org)
T2 cytokines IL-4 and IL-13 downregulate KLK5 in human bronchial epithelial cells. (bvsalud.org)
3-D laminin-rich extracellular matrix cultures of non-malignant human breast epithelial cells from a reduction mammoplasty were compared to malignant cells derived from the study's non-malignant cells. (rdworldonline.com)

The aim of our study was to analyze the hypoxic and pro-inflammatory microenvironment in GBMs and to demonstrate that in a stem/progenitor cell line derived from human glioblastoma (GBM-SCs), hypoxia activates a coordinated inflammatory response, evidencing an invasive and migratory phenotype. (biomedcentral.com)

low-affinity glucose transporter HXT4. (lbl.gov)
Low-affinity glucose transporter LGT1. (lbl.gov)
High affinity monosaccharide (KM ≈ 20 µM):H+ symporter, Stp6 (takes up glucose, 3-O-methylglucose, mannose, fructose, galactose and to a lesser extent, xylose and ribulose. (lbl.gov)

Vitamin C functions as a cofactor in many enzymatic reactions in animals (including humans) that mediate a variety of essential biological functions, including wound healing and collagen synthesis. (wikipedia.org)
The Glucose Transporter Inhibitor II controls the biological activity of Glucose Transporter. (emdmillipore.com)

BAY-876 ist ein potenter, selektiver und oral wirksamer glucose transporter 1 (GLUT1) -Inhibitor mit einem IC 50 von 2 nM. (medchemexpress.com)
BAY-876 is an orally active and selective glucose transporter 1 (GLUT1) inhibitor with an IC 50 of 2 nM. (medchemexpress.com)

We previously reported that alcohol inhibits glucose transport across the blood-brain barrier (BBB), leading to BBB dysfunction and neurodegeneration. (njit.edu)
One study on transgenic mice with human APP717(associated with familial AD) displayed subcellular neurodegeneration similar to those observed in AD, including dystrophic neurites, disruption of synaptic junction, and intracellular amyloid and reactive gliosis. (hstreasures.com)

Only partial data are available for the human tumor glioblastoma multiforme (GBM). (biomedcentral.com)
From each of 10 human solid glioblastomas, clinically and histopathologically characterized, we obtained three surgical samples taken from the center and the periphery of the tumor, and from adjacent host normal tissue. (biomedcentral.com)

Our understanding of the role of glucose transport in the lung and the mechanisms that regulate glucose movement across the human lung epithelium lags far behind that of the gut and kidney. (ersjournals.com)

cells, which oxidised glucose using mitochondria. (researchgate.net)

Suppressing glucose uptake leads to a phenotypic reversion of malignant cells (they look normal) while not affecting the non-malignant cells. (rdworldonline.com)
We suggest that, as has been previously described for prostatic and mammary carcinoma, in human glioblastoma acquisition of a proinflammatory phenotype may be relevant for malignant progression. (biomedcentral.com)

It is only recently that we and others have begun to investigate glucose transport as an important mechanism for maintaining a nutrient-depleted environment in the lung lumen to limit the growth of pathogenic organisms. (ersjournals.com)

The glucose/fructose:H+ symporter, STP13 (sugar transport protein 13). (lbl.gov)

For the first time, scientists have found a connection between abnormalities in how the brain breaks down glucose and the severity of the signature amyloid plaques and tangles in the brain, as well as the onset of eventual outward symptoms, of Alzheimer's disease. (nih.gov)

A high concentration of glucose also reduces the manufacture of leptin [LEPR] receptors, as is shown above. (ironmagazine.com)

Negative effects of high glucose exposure in human gonadotropin-releasing hormone neurons. (ironmagazine.com)
Our results indicate that ethanol exposure decreases the uptake of D-(2- 3 H)-glucose by human astrocytes and neurons. (njit.edu)
Our results indicate that ethanol exposure decreases the uptake of D-(2-3H)-glucose by human astrocytes and neurons. (njit.edu)

For some time, researchers have thought about the possible links between how the brain processes glucose and Alzheimer's," said NIA Director Richard J. Hodes, M.D. "Research such as this involves new thinking about how to investigate these connections in the intensifying search for better and more effective ways to treat or prevent Alzheimer's disease. (nih.gov)
Scientists found potential connections between problems with how the brain processes glucose and Alzheimer's disease pathology and symptoms. (globalhealthnewswire.com)

If you have too much glucose in your blood, your body produces less testosterone. (ironmagazine.com)
Blood-borne glucose is an essential energy source for the adult human brain. (epilepsygenetics.net)
All analyses were adjusted for age and gender, with subsequent analyses for baseline values of LV function and traditional risk factors of heart failure (i.e. cholesterol, HbA1c, cigarette smoking, systolic blood pressure, use of glucose-, blood pressure-, and lipid lowering medication and BMI). (easdec.org)
Finally, the team checked blood glucose levels in study participants years before they died, finding that greater increases in blood glucose levels correlated with greater brain glucose levels at death. (nih.gov)

This Review evaluates current techniques used to investigate human brain function, discusses the successes and limitations of these techniques to test hypotheses about causal mechanisms, and looks to future directions and implementation of these techniques in real-world problems. (natureasia.com)
These findings suggest that deprivation of glucose in brain cells contributes to neurotoxicity in alcohol abusers, and highlights ALC as a potential therapeutic agent to prevent the deleterious health conditions caused by alcohol abuse. (njit.edu)
Led by Madhav Thambisetty, M.D., Ph.D., investigator and chief of the Unit of Clinical and Translational Neuroscience in the NIA's Laboratory of Behavioral Neuroscience, researchers looked at brain tissue samples at autopsy from participants in the Baltimore Longitudinal Study of Aging (BLSA), one of the world's longest-running scientific studies of human aging. (nih.gov)
Researchers measured glucose levels in different brain regions, some vulnerable to Alzheimer's disease pathology, such as the frontal and temporal cortex, and some that are resistant, like the cerebellum. (nih.gov)

In a perfused, fluid-filled, adult rat lung model, ASL glucose was estimated to be 0.5 mM when perfusate glucose was 10 mM [ 4 ]. (ersjournals.com)

1 H-[ 13 C] MRS, i.e., indirect detection of signals from 13 C-coupled 1 H, together with infusion of 13 C-enriched glucose has provided insights into the coupling between neurotransmission and glucose oxidation. (frontiersin.org)

Inhibition of glucose uptake correlates with a reduction in glucose transporter protein expression (GLUT1 in astrocytes and GLUT3 in neurons). (njit.edu)

In this study, we hypothesized that ethanol (EtOH)-mediated disruption in glucose uptake would deprive energy for human astrocytes and neurons inducing neurotoxicity and neuronal degeneration. (njit.edu)

They experimented with human cells that produce the gonadotropin-releasing hormone [GnRH]. (ironmagazine.com)
In this study, we investigated the high glucose effects in the human GnRH-secreting FNC-B4 cells. (ironmagazine.com)
In immortalised human airway cells (line H441) with 10 mM glucose in the basolateral medium, apical ASL glucose was 0.24±0.07 mM [ 10 ]. (ersjournals.com)

If you want to boost your testosterone level in a natural way, make sure you don't develop Type-2 diabetes and avoid extremely high glucose peaks. (ironmagazine.com)
high confidence medium confidence low confidence transporter - transporters and PTS systems are shaded because predicting their specificity is particularly challenging. (lbl.gov)

Anatomy13

Organisms3

Diseases2

Chemicals and Drugs50

Analytical, Diagnostic and Therapeutic Techniques and Equipment6

Phenomena and Processes15

Disciplines and Occupations1

Information Science3

Metabolism12

GLUT1 and GLUT31

Glut5

Antibody10

Examined the expression of glucose1

GLUT44

SLC2A32

Facilitative glucose1

Elisa8

Neurons and astrocytes1

Mammalian cells1

Proteins1

GLUT81

SGLT1

Genes2

Insulin3

Gluts1

Homeostasis2

Concentrations11

Assay3

Glycolysis4

Lactate3

Deleterious direct contribution2

Pathways5

Metabolic5

Facilitator1

Testis2

Epithelial cells5

Hypoxia1

Affinity3

Biological2

Inhibitor2

Neurodegeneration2

Tumor2

Regulate1

Mitochondria1

Malignant2

Pathogenic1

Fructose1

Abnormalities1

Concentration1

Exposure3

Processes2

Blood4

Brain4

Adult1

Detection1

Reduction1

Neuronal1

Cells3

High2