• GLP-1 has a role in glycemic control by inducing glucose-dependent insulin secretion from β-cells and inhibiting glucagon release from α-cells in the pancreas. (justia.com)
  • Glucagon-like peptide-1 (GLP-1) is an important endogenous incretin hormone, which stimulates glucose-dependent insulin secretion from the pancreatic islet cells and supports glucose homeostasis [ 7 ], as well as stimulating β -cell proliferation and inhibiting β -cell apoptosis, increasing insulin sensitivity, and inhibiting glucagon secretion and gastrointestinal motility [ 7 - 10 ]. (hindawi.com)
  • Ligand-dependent activation of the G-protein coupled receptor 119 (GPR119) lowers blood glucose via glucose-dependent insulin secretion and intestinal glucagon-like peptide-1 production. (biomedcentral.com)
  • Involved in glucose homeostasis through induction of glucagon secretion at low glucose levels. (epigentek.com)
  • Proliferation of insulin and glucagon positive cells and the average counts of inflammatory cells in the islets did not differ between groups. (unipd.it)
  • IHC studies optimize staining protocols for specific target expression/profiling in human and animal tissues that can be used for a variety of reasons, such as proliferation/apoptosis studies, immuno-oncology, ocular tissues, tumor profiling and tissue cross reactivity. (labcorp.com)
  • GPR119 expression was assessed by real-time qPCR and immunohistochemistry in human breast cancer cell lines and breast cancer tissues. (biomedcentral.com)
  • Formalin-fixed, paraffin-embedded pancreatic samples were collected from post-mortem examination of 37 diabetic and 20 control cats, selected to be matched for age, sex, breed and body weight. (unipd.it)
  • β1 integrin is essential for pancreatic beta-cell development and maintenance in rodents and humans. (oncotarget.com)
  • The present disclosure relates to glucagon-like peptide-1 receptor (GLP-1R) agonists and methods of treating or preventing allergic inflammation and/or methods of treating or preventing respiratory syncytial virus (RSV) infection. (justia.com)
  • Disclosed herein are methods of treating allergic disorders and respiratory syncytial virus (RSV) infections with a glucagon-like peptide-1 receptor (GLP-1R) agonist. (justia.com)
  • In some aspects, disclosed herein is a method of treating or preventing an allergic disease in a subject who has, or is at risk of developing an allergic disease, comprising administering to the subject a therapeutically effective amount of a glucagon-like peptide-1 receptor (GLP-1Rt) agonist. (justia.com)
  • The pharmacodynamics of ALT-801, a GLP-1/glucagon receptor dual agonist optimized for NASH and weight loss, were compared to semaglutide (GLP-1 receptor agonist) and elafibranor (peroxisome proliferator-activated receptor, PPAR-α/δ, agonist) in a biopsy-confirmed, diet-induced obese (DIO) mouse model of NASH (DIO-NASH). (researchsquare.com)
  • Beyond the GLP-1 receptor (GLP-1R) agonists, unimolecular peptide dual agonists activating both GLP-1R and the glucagon receptor (GCGR) have been developed with the aim to achieve superior therapeutic benefits versus single agonist peptides 12 , 13 . (researchsquare.com)
  • Exendin-4 (E4), a long-acting agonist of the hormone glucagon-like peptide 1 receptor (GLP-1R), is administered to treat type II diabetes in the clinical setting and also shows a neuroprotective effect. (molvis.org)
  • from two sufferers had been cotransplanted with HLA-mismatched human being islets into immunodeficient rodents. (insulin-receptor.info)
  • Experimental design Islet cell isolation and culture Freshly isolated human islets and those kept in culture for a week had been pelleted by centrifugation, set in natural buffered formalin and inserted in paraffin for light microscopy or set in glutaraldehyde for ultrastructural research. (healthanddietblog.info)
  • The 48 endocrine part (Islets of Langerhans) secretes hormones like insulin, glucagon, somatostatin, and 49 polypeptide (3), while the exocrine part releases digestive enzymes (4). (myessaywriters.co)
  • In vitro tracer uptake in endocrine cells (INS-1 and human islets), but not PANC1 and exocrine cells, was mediated specifically by intracellular conversion into serotonin. (snmjournals.org)
  • Uptake of 11 C-5-HTP in the normal human pancreas is generally high, but this has not previously been systematically investigated despite the fact that islets and some neuronal tissue constitute the only amine precursor uptake and decarboxylase tissue within the pancreas. (snmjournals.org)
  • Specifically, islet cells were identified by staining with insulin, glucagon and chromogranin antibodies, soft tissue components by staining with actin (blood vessel walls, myoepithelial cells), vimentin (connective tissue) and factor VIII (endothelial cells) antibodies. (healthanddietblog.info)
  • Accordingly, immunohistochemistry with specific antibodies shows that HMGCS2 is expressed mainly in differentiated cells of human colonic epithelium. (aacrjournals.org)
  • There are many 50 similarities in the exocrine and endocrine parts between the rat and human pancreas (5,6,7,8,9) 51 and this make the rats more preferred in our experimental studies. (myessaywriters.co)
  • The endocrine cell population accounts for only a small part of the pancreas but is of paramount importance for the human metabolism. (snmjournals.org)
  • Results: The liver cells were found to possess preproinsulin mRNA, translate (pro)insulin in vivo and release it when exposed to 100 nmol/l glucagon in vitro. (deepdyve.com)
  • Uptake of 11 C-5-HTP, and its specificity for key enzymes in the serotonergic metabolic pathway, was assessed in vitro (INS-1 and PANC1 cells and human islet and exocrine preparations) and in vivo (nonhuman primates and healthy and diabetic rats). (snmjournals.org)
  • Finally, evidence of an increased proliferation rate of acinar cells, as reported in humans, could be associated with chronic pancreatitis as well as with transdifferentiation into islet cells. (unipd.it)
  • Human recombinant protein fragment corresponding to amino acids 29-212 of human IL-6 produced in E.coli. (thermofisher.com)
  • Glucagon-like peptide-1 (GLP-1) is a peptide hormone synthesized and released by enteroendocrine L-cells in the ileum and large intestine following oral food intake. (justia.com)
  • A synthetic peptide from internal cytoplasmic region of human Zinc transporter 5 (SLC30A5) conjugated to blue carrier protein was used as the antigen. (osenses.com)
  • The constructed model closely approximated the characteristics of human NASH and evaluation of its immune response signature, showed that the innate immune response was more pronounced than adaptive immunity. (biomedcentral.com)
  • Animal models that are as similar as possible to human NASH disease characteristics are needed for pathogenesis and drug development. (biomedcentral.com)
  • Human insulin and rat insulin standards for producing liver cells must not be destroyed by the the in-house radioimmunoassay (RIA) were obtained immune system or else this could lead to liver damage. (deepdyve.com)
  • studied the mechanisms of 11 C-5-HTP uptake in cell lines derived from rodent insulinoma (INS-1) and human ductal cells (PANC1) and reported DDC- and MAO-A-specific uptake in INS-1 but not in PANC1 cells ( 10 ). (snmjournals.org)
  • We performed FACS to obtain the purified - and -cells from Ins1-mRFP (34) and glucagon-Cre/Rosa26R-YFP (35) mice, respectively. (biotech2012.org)
  • IHC image of AIMP1 Polyclonal Antibody diluted at 1:200 and staining in paraffin-embedded human glioma cancer performed on a Leica Bond™ system. (epigentek.com)
  • Accordingly, the expression of human HMGCS2 is down-regulated in 90% of Myc-dependent colon and rectum tumors. (aacrjournals.org)
  • While the use of glucagon-like peptide1 (GLP-1) analogs in the control of diabetes has been associated with appetite suppression and reduced food intake, substantial numbers of patients exhibit gastrointestinal side effects (nausea, emesis) and average weight loss has ranged from 1-7% in studies leading to approval 6 . (researchsquare.com)
  • High-fat diet (HFD) consumption can have serious adverse effects on human metabolic and immune health. (biomedcentral.com)
  • Cell tradition and reagents We acquired three metastatic melanoma cell lines, A375, SB2, and WM1727A, from your American Type Tradition Collection (Manassas, VA). Normal human pDCs were from MatTek Corporation (Ashland, MA) and cultured in the manufacturers Corticotropin-releasing factor (CRF) DC-MM growth medium according to their instructions. (techieindex.net)
  • The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA and further post fixed overnight before being processed for paraffin embedding. (osenses.com)
  • Stage-specific embryonic antigen 4 (SSEA-4), an early embryonic glycolipid antigen, is expressed in cells derived from adult human pancreas exocrine tissue. (biomedcentral.com)
  • Human adult partial pancreas tissues were obtained from different donors and cultured in vitro. (biomedcentral.com)
  • SSEA-4 + and CA19-9 + cells were isolated from adult human pancreas exocrine cells using magnetic-activated cell sorting, and gene expression was validated by quantitative polymerase chain reaction. (biomedcentral.com)
  • SSEA-4-expressing cells were detected in isolated pancreas exocrine cells from adult humans. (biomedcentral.com)
  • SSEA-4-expressing cells in the adult human pancreas may have the potential for regeneration of the pancreas and may be used as a source of stem/progenitor cells for pancreatic cell lineage-specific differentiation. (biomedcentral.com)
  • In the adult human pancreas, Afrikanova et al. (biomedcentral.com)
  • GIPR expression has been reported in human bone, fetal adrenal, and pancreas. (ihcworld.com)
  • Western Blot: PDHX Antibody [NB100-1496] - Western blot analysis of Human Pancreas lysate (35ug protein in RIPA buffer) using NB100-1496 (1ug/ml). (novusbio.com)
  • Immunohistochemistry: PDHX Antibody [NB100-1496] - staining of paraffin embedded Human Pancreas. (novusbio.com)
  • 50 kDa band observed in human pancreas lysate (calculated MW of 54.2 kDa band according to NP_003468.1). (novusbio.com)
  • Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 18034-1-AP (GIP Antibody) at dilution of 1:200 (under 10x lens). (ptglab.com)
  • Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 18034-1-AP (GIP Antibody) at dilution of 1:200 (under 40x lens). (ptglab.com)
  • Endocrine pancreas , the portions of the pancreas (the islets) that make and secrete insulin, glucagon, somatostatin and pancreatic polypeptide into the blood. (pancreapedia.org)
  • The gross anatomy of the human pancreas can vary. (pancreapedia.org)
  • That is why it is named as amphicrine gland The major part of pancreas corresponds to the exocrine part (98-99 % in humans). (uvigo.es)
  • 13. Design and Development of a Fully Synthetic Multiplex Ligation-Dependent Probe Amplification-Based Probe Mix for Detection of Copy Number Alterations in prostate cancer Formalin-Fixed, Paraffin-Embedded Tissue Samples. (nih.gov)
  • One hemisphere was formalin fixed and embedded in paraffin for immunohistochemical analysis. (nih.gov)
  • Program H&E staining were performed and immunohistochemical studies were carried out within the formalin- fixed, paraffin- embedded cells sections using a panel of the following antibodies: CD10 clone 56C6, CD34 clone QBEnd 10, polyclonal rabbit anti- human being CD 117(c-kit), polyclonal rabbit S-100, SMA 1A4 clone, HMB-45 clone HMB-45 and Vimentin clone V9, all antibodies provided by Dako. (biotechnologyconsultinggroup.com)
  • the other half were embedded sagittally in OCT and snap frozen for cryosectioning and immunohistochemistry. (nih.gov)
  • Immunohistochemistry was performed on paraffin-embedded tissue sections using monoclonal antibodies against human Ki67 (MIB-1, DAKO/Agilent, Santa Clara, CA, Agilent, Cat#: F726801) and phosphorylated-SMAD3 (phosphoS423? (arcticcirclescotland.com)
  • Glucagon is a 29 amino acid linear polypeptide produced by pancreatic islet A cells and by upper G.I. tract cells. (pharmacology2000.com)
  • Notably, a fraction of the T cells expressed the CD103 antigen, mirroring the recently reported presence of tissue resident memory T cells in the insulitis in humans with recent onset T1D. (springer.com)
  • At the Joslin Diabetes Center, paraffin sections were microwaved for antigen retrieval and immunostained with guinea pig anti-bovine insulin (1:200, Linco), rabbit anti-bovine glucagon (1:2000 M. Appel, University of Massachusetts), mouse anti-human Ki67 (1:200, Becton, Dickinson and Company), and rabbit anti-human CD3 (1:50, Dako). (medscape.com)
  • Adenosine receptors participate in the category of G protein-coupled receptors, which you can find four subtypes (A1, A2A, A2B and A3), which enjoy different jobs in regulating regular mobile physiology in a multitude of tissue including the human brain, heart and lungs [7]. (siamtech.net)
  • Another mechanism by which glucagon stimulates glycogenolysis involves glucagon interacting via different glucagon receptors on the same liver cell thus activating phospholipase C with subsequent increases in cytoplasmic Ca 2+ stimulating glycogenolysis. (pharmacology2000.com)
  • Diet-induced metabolic syndrome was found to be the closest model that at least shares the similar ethologic, and hence more representative of human pathophysiology of metabolic syndrome. (biomedcentral.com)
  • Serial sections of paraffin-embedded pancreatic autopsy samples from 72 recent-onset type 1 diabetes patients and up to 161 controls were immunostained for insulin, glucagon, vp1, double-stranded RNA activated protein kinase R (PKR) and MHC class I. (nih.gov)
  • The results presented unravel a previously unknown interplay between innate and acquired immunity in the formation of immunopathological events indistinguishable from those described in humans with recent onset T1D. (springer.com)
  • Reduced protein level of a requisite scaffolding protein of the initiation complex, eIF4G1, downstream of nutrients and insulin signaling is associated with diabetes in humans and mice. (diabetesjournals.org)
  • 15. Silver Nanoparticles Synthesized Using Carica papaya Leaf Extract (AgNPs-PLE) Causes Cell Cycle Arrest and Apoptosis in Human prostate (DU145) cancer Cells. (nih.gov)
  • iRNA expression profile during osteogenic differentiation of human adipose? (saiguotech.com)
  • 17. Modulating the dose-rate differently affects the responsiveness of human epithelial prostate - and mesenchymal rhabdomyosarcoma- cancer cell line to radiation. (nih.gov)
  • This product is for research use only and is not approved for use in humans or in clinical diagnosis. (novusbio.com)
  • One particular probable purpose is the fact that Ndfip1 deficiency causes GI pathology that manifests itself differently amongst mice and humans. (glucagon-receptor.com)
  • Nevertheless, it's clear that Ndfip1 prevents GI illness in mice and may well also avoid the improvement of GI illnesses in humans. (glucagon-receptor.com)
  • The influence of the environment was studied by transplanting human NPCs within the lateral ventricles (LV), compared with the prefrontal cortex (PFC) of immunocompetent mice. (bvsalud.org)
  • Establishment of a continuous untransfected human corneal endothelial cell line and its biocompatibility to denuded amniotic membrane. (saiguotech.com)
  • Figures 1A and 1B are two normal human pancreases from autopsies of adults. (pancreapedia.org)
  • The pancreases were procured by the National Disease Research Interchange and shipped to the nPOD Pathology Core (University of Florida, Gainesville, FL) where they were breadloafed into up to 20 blocks, fixed in 10% buffered formalin, and processed routinely for paraffin embedding. (medscape.com)
  • Human brain development starts at the embryonic stages, in utero, with unique properties at its neotenic stages. (bvsalud.org)
  • In humans, epidemiological evidence has associated BPA exposure in adults with higher risk of type 2 diabetes and heart disease. (nih.gov)
  • 7. The inhibitory effect of melatonin on human prostate cancer . (nih.gov)
  • 16. [Down-regulated PTTG1 expression promotes the senescence of human prostate cancer LNCaP-AI]. (nih.gov)
  • Hence, it can be seen that a combination of high carbohydrate and high fat diet produce a more human-like model. (biomedcentral.com)
  • The Joslin Committee on Human Subjects approved the study. (medscape.com)