• Geminin controls replication by binding to the licensing factor Cdt1, and is involved in neural differentiation. (prospecbio.com)
  • During the "M phase" of the cell cycle (mitosis) Geminin stabilizes the replication factor Cdt1 promoting DNA replication during the next cell cycle. (prospecbio.com)
  • Moreover, inhibition of Geminin during mitosis (by RNAi) causes destabilization of Cdt1 protein and impairment of DNA replication during the next cell cycle. (prospecbio.com)
  • At the start of the S-phase until late mitosis, geminin inhibits the replication factor Cdt1, preventing the assembly of the pre-replication complex. (wikipedia.org)
  • 2013. Geminin deploys multiple mechanisms to regulate Cdt1 before cell division thus ensuring the proper execution of DNA replication. (virosin.org)
  • Recent breakthroughs have uncovered more and more DNA replication licensing machinery proteins (ORC, Cdc6, Cdt1, geminin, etc.) functioning in other cell cycle events, including centrosome replication, mitotic events, transcription and so on. (intechopen.com)
  • We have investigated regulation of the origin licensing factors Cdc6, Cdt1, Mcm2 and Geminin in human somatic and germ cells. (dundee.ac.uk)
  • In striking contrast, we show that proliferation control during male gametogenesis is not linked to Cdc6 or Mcm2, but appears to be coordinated by the negative regulator Geminin with Cdt1 becoming rate-limiting in late prophase. (dundee.ac.uk)
  • MCM loss' zygotes were generated by preventing the factor geminin from being degraded as the presence of geminin stops Cdt1 directed loading of MCM during G1 phase (6). (biologists.com)
  • A dimerized coiled-coil domain and an adjoining part of geminin interact with two sites on Cdt1 for replication inhibition. (nii.res.in)
  • Geminin types a Cdt1 geminin complicated that regulates Cdt1, which initiates DNA replication licensing. (gabasignaling.com)
  • Geminin inhibits and stabilizes Cdt1. (gabasignaling.com)
  • The stoichiometry in the Cdt1 geminin complicated con trols regulation of DNA replication licensing. (gabasignaling.com)
  • Geminin is a 25 kDa nuclear protein, which inhibits DNA replication and is degraded during the mitotic phase of the cell cycle. (prospecbio.com)
  • In addition, Geminin directly interacts with Six3 and Hox homeodomain proteins during embryogenesis and inhibits their functions. (prospecbio.com)
  • Geminin also inhibits the chromatin remodeling things Brahma and Brg1 to retain an undifferentiated state and acts being a transcription repressor or corepressor. (gabasignaling.com)
  • Inhibition of Geminin during the "S phase" (by RNAi) results in an additional round of replication of portions of the genome. (prospecbio.com)
  • In many cancer cell lines, inhibition of geminin by RNA interference results in re-replication of portions of the genome, which leads to aneuploidy. (wikipedia.org)
  • In neural precursor cells, high levels of geminin prevent terminal differentiation. (wikipedia.org)
  • The dual roles of geminin during trophoblast proliferation and differentiation. (virosin.org)
  • Geminin is a repressor of origin licensing that blocks Mcm2-7 loading onto origins. (dundee.ac.uk)
  • Human genome codes for many other single strand DNA binding proteins and we are testing if novel mechanisms of checkpoint activation exist that do not require Replication Protein A. If such mechanisms exist we would like to understand which factors provide the role of DNA binding, how checkpoint proteins like Chk1 and p53 are activated and how signal transduction pathway stalls the cell cycle and DNA replication. (nii.res.in)
  • A large-scale binding and functional map of human RNA -binding proteins. (github.io)
  • A nuclear protein present in most eukaryotes and highly conserved across species, numerous functions have been elucidated for geminin including roles in metazoan cell cycle, cellular proliferation, cell lineage commitment, and neural differentiation. (wikipedia.org)
  • In addition, the potential for these genes to perform various cognitive roles during human brain evolutionary processes is discussed. (biomedcentral.com)
  • Coincidentally, geminin was also shown to expand the neural plate in the developing Xenopus embryo. (wikipedia.org)
  • Geminin promotes early neural fate commitment by hyperacetylating chromatin. (wikipedia.org)
  • Ultimately, geminin allows cells uncommitted to any particular lineage to acquire neural characteristics. (wikipedia.org)
  • When the interaction between geminin and SWI/SNF is eliminated, geminin's inhibition to this process is eliminated and neural precursors are allowed to differentiate. (wikipedia.org)
  • Geminin has also been shown to interact with the SWI/SNF chromatin remodeling complex. (wikipedia.org)
  • Geminin levels drop at the metaphase-anaphase transition of mitosis when it is degraded by the anaphase-promoting complex. (wikipedia.org)
  • Geminin has been found to be overexpressed in several malignancies and cancer cell lines, while there is data demonstrating that geminin acts as a tumor suppressor by safeguarding genome stability. (wikipedia.org)
  • The Nuclear Receptor family is a large group of ligand-activated transcription factors with 48 members presently identified in the human genome. (jnu.ac.in)
  • Our data demonstrate a striking sexual dimorphism in the mechanisms repressing origin licensing and preventing untimely DNA synthesis during meiosis I, implicating a pivotal role for Geminin in maintaining integrity of the male germline genome. (dundee.ac.uk)
  • Geminin antibody was purified from mouse ascitic fluids by protein-G affinity chromatography. (prospecbio.com)
  • Geminin antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. (prospecbio.com)
  • Geminin is a nuclear protein made up of about 200 amino acids, with a molecular weight of approximately 25 kDa. (wikipedia.org)
  • A human cytomegalovirus-encoded microRNA regulates expression of multiple viral genes involved in replication. (virosin.org)
  • The gene encoding GEMC1 maps to human chromosome 3, which is made up of about 214 million bases encoding over 1,100 genes. (mc1-r.com)
  • The used of a comprehensive dataset of gene profiles from all the major human leukocyte subsets enabled a novel and powerful means for identification of genes associated with single leukocyte subsets, or different immune paradigms. (gsea-msigdb.org)
  • We applied an assay that measures the stability of maintenance of an episomal plasmid in human tissue culture cells to screen for new DNA replication factors. (aacrjournals.org)
  • DNA synthesis occurred on either circular or linear plasmids coupled to the beads, but only DNA synthesis on the circular plasmid was inhibited by geminin and a. (shengsci.com)
  • In this assay hematopoietic cells are cultured in MyeloCultâ„¢ medium with supportive human or mouse stromal cell layers and tested for their ability to generate myeloid clonogenic progenitors for several weeks as assayed by replating the cells in a colony-forming unit (CFU) assay using MethoCultâ„¢ medium. (stemcell.com)
  • We previously demon strated that Scmh1 mediates a molecular interaction of PcG com plex one with geminin and that PcG complex one acts as an selleck chemicals E3 ubiquitin ligase for geminin to sustain the hematopoietic stem cell exercise. (gabasignaling.com)
  • In the present study we used Affymetrix oligonucleotide microarrays to produce gene transcription profiles for the major leukocyte types in humans. (gsea-msigdb.org)
  • In addition, increased genomic instability and tumorigenesis have been observed in geminin knockout mice in both the colon and lung. (wikipedia.org)
  • Conclusions there is growing evidence that l1 expression and moncton even complete retrotransposition muskegon occur in selected human cancers, suggesting that l1 may drive tumorigenesis. (marmisur.com)
  • However, the same is not true for primary and immortalized human cell lines, where other mechanisms exists to prevent DNA re-replication. (wikipedia.org)
  • Geminin is absent during G1 phase and accumulates through S, G2 phase and M phases of the cell cycle. (wikipedia.org)
  • In these cell lines, geminin knockdown leads to markedly slowed growth and apoptosis within several days. (wikipedia.org)
  • Since geminin knockdown leads to cell death in many cancer cell lines but not primary cell lines, it has been proposed as a potential therapeutic target for cancer treatment. (wikipedia.org)
  • Geminin, therefore, is an important player in ensuring that exactly one round of replication occurs during each cell cycle. (wikipedia.org)
  • Geminin pre vents rereplication from S phase to early M phase to make sure one particular round of DNA replication in a single cell cycle. (gabasignaling.com)
  • Das D, Tapryal N, Goswami S.K., Fox P.L, and Mukhopadhyay C.K. Regulation of Ceruloplasmin in human hepatic cells by redox active copper: Identification of a novel AP-1 site in ceruloplasmin gene. (jnu.ac.in)
  • Anti-human Geminin mAb is derived from hybridization of mouse SP2/0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human Geminin amino acids 1-209 purified from E. coli. (prospecbio.com)
  • 2003. Human cytomegalovirus infection leads to accumulation of geminin and inhibition of the licensing of cellular DNA replication. (virosin.org)
  • Human cytomegalovirus latent infection alters the expression of cellular and viral microRNA. (virosin.org)
  • The particular lipophilicity from the materials was assessed Mekinist by simply RP-HPLC in addition to their anti-proliferative activity ended up being assayed against the human SK-N-MC neuroepithelioma and HCT116 human being intestinal tract carcinoma cellular traces. (sodiumchannel-signal.com)
  • Differentially expressed epigenome modifiers, including aurora kinases A and B, in immune cells in rheumatoid arthritis in humans and mouse models. (rush.edu)
  • The C terminal putative nuclear localization signal domain acts also as an interaction do primary for geminin. (gabasignaling.com)
  • We developed a new single-molecule method to probe non-specific DNA interactions for two HMGB homologs: the human HMGB2 box A domain and yeast Nhp6Ap, along with chimeric mutants replacin. (shengsci.com)
  • Model organisms have been used in the study of geminin function. (wikipedia.org)
  • MicroRNAs (miRNAs), which function as post-transcriptional regulators, are utilized by human cytomegalovirus (HCMV) in its infection and pathogenesis. (virosin.org)
  • The cut-off to define low rad51 score was finalised by examining rad51 and geminin before unblinding to clinicopathological and response data. (marmisur.com)
  • Understanding how DNA replication is regulated in human cells can provide insight into cancer development and may reveal vulnerabilities that can be exploited therapeutically. (aacrjournals.org)
  • Notably, there is a chemokine receptor gene cluster and a variety of human cancer related loci on chromosome 3. (mc1-r.com)